Éditeur(s) : HAL CCSD Elsevier
Résumé : International audience
Molds and allergens are common indoor biocontaminants. The aims of this study were to assess the concentrations of common molds in indoor air and floor dust and the concentrations of house dust mite, cat and dog allergens in mattress dust in French dwellings, and to assess predictors of these concentrations. A sample of 150 houses in Brittany (western France) was investigated. Airborne Cladosporium and Penicillium were detected in more than 90% of the dwellings, Aspergillus in 46% and Alternaria in only 6% of the housings. Regarding floor dust samples, Cladosporium and Penicillium were detected in 92 and 80% of the housings respectively, Aspergillus in 49% and Alternaria in 14%. House dust mite allergens Der p1 and Der f1 were detected in 90% and 77% of the mattress dust samples respectively and Can f1 and Fel d1 in 37% and 89% of the homes. Airborne and dustborne mold concentrations, although not statistically correlated (except for Aspergillus) shared most of their predictors. Multivariate linear models for mold levels, explaining up to 62% of the variability, showed an influence of the season, of the age of the dwelling, of aeration habits, presence of pets, smoking, signals of dampness, temperature and relative humidity. Allergens in the dust of the mattress were strongly related to the presence of pets and cleaning practices of bedsheets, these factors accounting for 60% of the variability. This study highlights ubiquitous contamination by molds and underlines complex interaction between outdoor and indoor sources and factors
ISSN: 0048-9697

hal-01165440
https://hal-univ-rennes1.archives-ouvertes.fr/hal-01165440
https://hal-univ-rennes1.archives-ouvertes.fr/hal-01165440/file/Molds%20and%20allergens%20in%20French%20dwellings%20rev2_clean%20version.pdf
DOI : 10.1016/j.scitotenv.2015.06.039
PUBMED : 26094801

Éditeur(s) : HAL CCSD Elsevier
Résumé : International audience
Our goal was to determine if the US Environmental Relative Moldiness Index (ERMI) scale might have application in France. Twenty homes in Brittany, north western region of France were classified by inspection as "Moldy" or "Non-Moldy". Dust and air samples were collected (MiTest sampler or Coriolis sampler, respectively) from each home and analyzed by quantitative polymerase chain reaction (QPCR) for the 36 fungi that make-up the ERMI. Inspection and ERMI values provided a consistent assessment for 90% of the homes. Two homes originally classified as "Non-Moldy" were found to fit better into the "Moldy" category based on the QPCR analysis and the ERMI. Dust and air samples analyzed by QPCR provided similar fungal contamination assessments. In conclusion, a metric like the ERMI describes mold burdens in homes on a continuum, as opposed to the frequently used dichotomous approach (moldy vs. non-moldy). Although a larger, random national sampling of French homes is needed, these results suggest that these same 36 fungi may be useful in creating an ERMI for France.
ISSN: 1438-4639

inserm-00817898
http://www.hal.inserm.fr/inserm-00817898
http://www.hal.inserm.fr/inserm-00817898/document
http://www.hal.inserm.fr/inserm-00817898/file/Gangneux-Possible-Application-accepted.pdf
DOI : 10.1016/j.ijheh.2012.06.004

Éditeur(s) : HAL CCSD Wiley-Blackwell
Résumé : International audience
Respiratory illnesses have been linked to children's exposures to water-damaged homes. Therefore, understanding the microbiome in water-damaged homes is critical to preventing these illnesses. Few studies have quantified bacterial contamination, especially specific species, in water-damaged homes. We collected air and dust samples in twenty-one low-mold homes and twenty-one high-mold homes. The concentrations of three bacteria/genera, Stenotrophomonas maltophilia, Streptomyces sp., and Mycobacterium sp., were measured in air and dust samples using quantitative PCR (QPCR). The concentrations of the bacteria measured in the air samples were not associated with any specific home characteristic based on multiple regression models. However, higher concentrations of S. maltophilia in the dust samples were associated with water damage, that is, with higher floor surface moisture and higher concentrations of moisture-related mold species. The concentrations of Streptomyces and Mycobacterium sp. had similar patterns and may be partially determined by human and animal occupants and outdoor sources of these bacteria.
Indoor Air

hal-00877905
https://hal.archives-ouvertes.fr/hal-00877905
DOI : 10.1111/ina.12035
PUBMED : 23397905

Résumé : The slides were taken on collecting trips sponsored by the William L. Bryant Foundation, where books, music and art indigenous to the regions were gathered. The are organized by geographical location.
This building’s architecture features Bahama window shutters (which are attached by a top hinge designed for sun and weather protection), quoins (a Caribbean Georgian architectural element on the corner of buildings), and plaster moldings around windows. Slide labeled Bryant Nassau Mar .'46.
Bahamas -- North America -- New Providence Island

Droits : All rights to images are held by the respective holding institution. This image is posted publicly for non-profit educational uses, excluding printed publication. For permission to reproduce images and/or for copyright information contact Special Collections & University Archives, University of Central Florida Libraries, Orlando, FL 32816 phone (407) 823-2576, email: speccoll@mail.ucf.edu
CFM1972_01a
Sheet 16:11
http://ufdc.ufl.edu/AA00029452/00001

Éditeur(s) : HAL CCSD
Résumé : Molds are common contaminants in indoor environments. Health effects associated with these microscopic fungi are multiple, including irritations, allergic reactions, infections and toxicity. Depending on the exposure level and the vulnerability of the populations, fungi may represent different public health risks. For example, damp and moldy dwellings are associated with chronic, respiratory diseases such as asthma and allergic rhinitis. However, the lack of valid tools for accurately measuring the environmental fungal exposure is one of the main challenges to better understanding the role of fungal products and particles in human health. In this thesis, diverse sampling techniques and analytical methods were evaluated for measuring the fungal contamination in the air, on surfaces and in settled dust. A quantitative PCR-based index was notably tested and compared with other approaches to assess fungal exposure in dwellings. By collecting and analyzing settled floor dust, this index successfully distinguished between contaminated and non-contaminated homes in Brittany, France. Quantitative PCR and this index are promising techniques for fungal exposure assessment in indoor environments. Further validation of these methods may lead to disease prevention approaches, particularly for vulnerable populations.
Les moisissures sont des micro-organismes présents dans tous les environnements intérieurs. Les effets sur la santé associés à ces champignons microscopiques sont multiples : irritations, réactions immunoallergiques, infections et effets toxiques. Selon le niveau d'exposition et la vulnérabilité des populations, les moisissures peuvent représenter des risques plus ou moins importants pour la santé publique. Les logements humides et moisis ont par exemple été associés à des maladies chroniques respiratoires telles que l'asthme et les rhinites allergiques. Cependant, le manque d'outils valides permettant d'évaluer quantitativement l'exposition fongique environnementale constitue une des principales difficultés pour mieux appréhender le rôle des particules et des composés fongiques sur la santé humaine. Lors de ce travail de thèse, diverses techniques d'échantillonnage et méthodes d'analyses ont été évaluées afin de mesurer la contamination fongique de l'air, des surfaces et des poussières. Un indice moléculaire basé sur la technique de PCR quantitative a notamment été testé et comparé à d'autres approches pour mesurer l'exposition fongique dans des logements. Après analyse des poussières domestiques collectées au sol, cet indice a permis de discriminer les habitats avec ou sans moisissures visibles. La quantification de groupes fongiques par PCR quantitative s'avère une technique prometteuse pour la mesure de l'exposition aux moisissures dans les environnements intérieurs. Outre le développement et la validation d'outils de mesure, une démarche globale de prévention du risque fongique semble nécessaire, particulièrement chez les populations vulnérables.
https://tel.archives-ouvertes.fr/tel-00768444

Droits : info:eu-repo/semantics/OpenAccess
tel-00768444
https://tel.archives-ouvertes.fr/tel-00768444
https://tel.archives-ouvertes.fr/tel-00768444/document
https://tel.archives-ouvertes.fr/tel-00768444/file/MA_heust_Delphine.pdf

Éditeur(s) : HAL CCSD Taylor & Francis
Résumé : International audience
Portable microbial samplers are useful for detecting microorganisms in the air. However, limited data are available on their performance when sampling airborne biological agents in a routine practice. We compared bacterial and fungal concentrations obtained in field conditions using three impactor samplers with different designs (AES Chemunex Sampl'Air, bioMérieux Air Ideal, and Sartorius AirPort MD8/BACTair). The linearity of mold collection was tested in the range of 100 L to 1000 L, and all the devices had a correlation coefficient higher than 0.95. For optimal comparison of the samplers, we performed experiments in different hospital rooms with varying levels of air biocontamination. Each sampling procedure was repeated to assess reproducibility. No significant difference between the samplers was observed for the mold concentrations on Sabouraud agar, whereas Sampl'Air collected significantly more bacteria on tryptic soy agar than Air Ideal or BACTair at one of the sites. Impactor location in the room was nevertheless associated with the variability observed with the three samplers at the highest microbial concentration levels. On the basis of their performance, autonomy and simplicity of use, these three impactors are suitable for routine indoor evaluation of microbial air contamination.
ISSN: 1545-9624

hal-00874614
https://hal.archives-ouvertes.fr/hal-00874614
DOI : 10.1080/15459624.2013.800955
PUBMED : 23799861

Éditeur(s) : HAL CCSD Taylor & Francis
Résumé : International audience
Abstract Indoor fungal contamination has been associated with a wide range of adverse health effects, including infectious diseases, toxic effects and allergies. The diversity of fungi contributes to the complex role that they play in indoor environments and human diseases. Molds have a major impact on public health, and can cause different consequences in hospitals, homes and workplaces. This review presents the methods used to assess fungal contamination in these various environments, and discusses advantages and disadvantages for each method in consideration with different health risks. Air, dust and surface sampling strategies are compared, as well as the limits of various methods are used to detect and quantify fungal particles and fungal compounds. In addition to conventional microscopic and culture approaches, more recent chemical, immunoassay and polymerase chain reaction (PCR)-based methods are described. This article also identifies common needs for future multidisciplinary research and development projects in this field, with specific interests on viable fungi and fungal fragment detections. The determination of fungal load and the detection of species in environmental samples greatly depend on the strategy of sampling and analysis. Quantitative PCR was found useful to identify associations between specific fungi and common diseases. The next-generation sequencing methods may afford new perspectives in this area.
ISSN: 1040-841X

hal-00874670
https://hal.archives-ouvertes.fr/hal-00874670
DOI : 10.3109/1040841X.2013.777687
PUBMED : 23586944

Éditeur(s) : HAL CCSD
Résumé : National audience
Introduction Une exposition domiciliaire fongique élevée quantifiée par l’index environnemental moléculaire Environmental Relative Moldiness Index (ERMI) est associée au développement de l’asthme dans l’enfance. Dans ce travail, nous avons utilisé cette technique basée sur la quantification moléculaire de 36 champignons dans les poussières pour comparer sa valeur dans l’air à la détection par séquençage haut-débit NGS. Matériels et méthodes Des prélèvements de poussière et d’air ont été collectés dans 20 logements étiquetés « moisis » et 20 logements « non moisis » par les méthodes suivantes : 2 m2 de poussières prélevées à l’aide d’un embout Mitest™ positionné sur un aspirateur ; 3 m3 d’air prélevés par le collecteur cyclonique Coriolis® (Bertin Technologies, France). Tout d’abord, nous avons validé l’ERMI dans l’air versus les poussières, puis comparé dans les prélèvements d’air l’ERMI le séquençage NGS (10 échantillons « moisis » versus 10 échantillons « non moisis »). La cible était le gène 18S séquencé par pyroséquençage à l’aide du système Roche 454. Résultats et discussion Une corrélation entre l’ERMI « poussières » et l’ERMI « air » a été observée avec une valeur significative du test Kendall's tau. Les moisissures intérieures les plus identifiées étaient Aspergillus restrictus, A. fumigatus, A. versicolor, Aureobasidium pullulans Penicillium crustosum, Wallemia sebi, auxquelles s’associaient des moisissures classiquement d’extérieur, Cladosporium cladosporoides, Cladosporium herbarum. L’analyse des séquences obtenues par pyroséquençage était superposable à l’observation par ERMI. Les 3 genres les plus représentés dans l’air intérieur étaient Aspergillus, Penicillium et Cladosporium. Toutefois, quelques genres non inclus dans le panel ont pu être détectés en quantité non négligeable, à l’image des levures notamment du genre Cryptococcus. L’ERMI et le NGS apparaissent donc utiles et corrélés pour la quantification de la charge fongique environnementale dans l’air. L’ERMI a l’avantage d’être une technique rapide, mais sa limite est de cibler a priori 36 genres fongiques (définis initialement aux États-Unis). L’analyse NGS permet d’identifier d’autres genres potentiellement pertinents et donc d’adapter les cibles pour un ERMI « français »
Congrès de la SFMM
Talence-Bordeaux, France

hal-01198651
https://hal-univ-rennes1.archives-ouvertes.fr/hal-01198651
DOI : 10.1016/j.mycmed.2015.06.013

Éditeur(s) : HAL CCSD BioMed Central
Résumé : International audience
ABSTRACT: Environmental exposures during pregnancy and early life may have adverse health effects. Single birth cohort studies often lack statistical power to tease out such effects reliably. To improve the use of existing data and to facilitate collaboration among these studies, an inventory of the environmental exposure and health data in these studies was made as part of the ENRIECO (Environmental Health Risks in European Birth Cohorts) project. The focus with regard to exposure was on outdoor air pollution, water contamination, allergens and biological organisms, metals, pesticides, smoking and second hand tobacco smoke (SHS), persistent organic pollutants (POPs), noise, radiation, and occupational exposures. The review lists methods and data on environmental exposures in 37 European birth cohort studies. Most data is currently available for smoking and SHS (N=37 cohorts), occupational exposures (N=33), outdoor air pollution, and allergens and microbial agents (N=27). Exposure modeling is increasingly used for long-term air pollution exposure assessment; biomonitoring is used for assessment of exposure to metals, POPs and other chemicals; and environmental monitoring for house dust mite exposure assessment. Collaborative analyses with data from several birth cohorts have already been performed successfully for outdoor air pollution, water contamination, allergens, biological contaminants, molds, POPs and SHS. Key success factors for collaborative analyses are common definitions of main exposure and health variables. Our review emphasizes that such common definitions need ideally be arrived at in the study design phase. However, careful comparison of methods used in existing studies also offers excellent opportunities for collaborative analyses. Investigators can use this review to evaluate the potential for future collaborative analyses with respect to data availability and methods used in the different cohorts and to identify potential partners for a specific research question.
ISSN: 1476-069X

inserm-00785248
http://www.hal.inserm.fr/inserm-00785248
http://www.hal.inserm.fr/inserm-00785248/document
http://www.hal.inserm.fr/inserm-00785248/file/1476-069X-12-8.pdf
DOI : 10.1186/1476-069X-12-8

Éditeur(s) : HAL CCSD Elsevier
Résumé : International audience
The Environmental Relative Moldiness Index (ERMI) is a DNA-based metric developed to describe the fungal contamination in US dwellings. Our goal was to determine if the ERMI values in dwellings in north western France were correlated with other measures of fungal contamination. Dust and air samples were obtained from 40 dwellings and analyzed by quantitative PCR and/or by culturing. These dwellings were also inspected and the amount of visible fungal growth estimated in m(2). The ERMI values in these dwellings ranged from -2.7 to 28.8 and the fungal contamination estimates ranged from 0 to 20 m(2). The 40 dwellings were divided into those with a low (<6) or high (>8) ERMI values (n=20 in each). The average ERMI value was 15.70 for the high ERMI compared to 2.68 for the low ERMI dwellings. ERMI values were correlated (significant Kendall's tau values) with concentrations of fungi in air samples analyzed by QPCR or culturing. ERMI values were also correlated (significant Kendall's tau values) with the visible estimates of fungal contamination. Older dwellings were more likely to have higher ERMI values. These results suggest that the ERMI dust sample, which is quick and easy to collect, may be useful in making decisions about reducing fungal exposures in homes.
ISSN: 0048-9697

hal-00874733
https://hal.archives-ouvertes.fr/hal-00874733
DOI : 10.1016/j.scitotenv.2012.08.085
PUBMED : 23022719