Éditeur(s) : HAL CCSD
Résumé : International audience
AGU Fall Meeting 2014
San Francisco, United States

insu-01119759
https://hal-insu.archives-ouvertes.fr/insu-01119759

Éditeur(s) : HAL CCSD Landes Bioscience
Résumé : International audience
In 2008 we published the first set of guidelines for standardizing research in autophagy. Since then, research on this topic has continued to accelerate, and many new scientists have entered the field. Our knowledge base and relevant new technologies have also been expanding. Accordingly, it is important to update these guidelines for monitoring autophagy in different organisms. Various reviews have described the range of assays that have been used for this purpose. Nevertheless, there continues to be confusion regarding acceptable methods to measure autophagy, especially in multicellular eukaryotes. A key point that needs to be emphasized is that there is a difference between measurements that monitor the numbers or volume of autophagic elements (e.g., autophagosomes or autolysosomes) at any stage of the autophagic process vs. those that measure flux through the autophagy pathway (i.e., the complete process); thus, a block in macroautophagy that results in autophagosome accumulation needs to be differentiated from stimuli that result in increased autophagic activity, defined as increased autophagy induction coupled with increased delivery to, and degradation within, lysosomes (in most higher eukaryotes and some protists such as Dictyostelium) or the vacuole (in plants and fungi). In other words, it is especially important that investigators new to the field understand that the appearance of more autophagosomes does not necessarily equate with more autophagy. In fact, in many cases, autophagosomes accumulate because of a block in trafficking to lysosomes without a concomitant change in autophagosome biogenesis, whereas an increase in autolysosomes may reflect a reduction in degradative activity. Here, we present a set of guidelines for the selection and interpretation of methods for use by investigators who aim to examine macroautophagy and related processes, as well as for reviewers who need to provide realistic and reasonable critiques of papers that are focused on these processes. These guidelines are not meant to be a formulaic set of rules, because the appropriate assays depend in part on the question being asked and the system being used. In addition, we emphasize that no individual assay is guaranteed to be the most appropriate one in every situation, and we strongly recommend the use of multiple assays to monitor autophagy. In these guidelines, we consider these various methods of assessing autophagy and what information can, or cannot, be obtained from them. Finally, by discussing the merits and limits of particular autophagy assays, we hope to encourage technical innovation in the field.
Autophagy

hal-00735751
https://hal.archives-ouvertes.fr/hal-00735751
PUBMED : 22966490

Éditeur(s) : HAL CCSD Nature Publishing Group
Résumé : International audience
Assessing seismic hazards remains one of the most challenging scientific issues in Earthsciences. Deep tectonic processes are classically considered as the only persistentmechanism driving the stress loading of active faults over a seismic cycle. Here we show via amechanical model that erosion also significantly influences the stress loading of thrust faultsat the timescale of a seismic cycle. Indeed, erosion rates of about B0.1–20mmyr1, asdocumented in Taiwan and in other active compressional orogens, can raise the Coulombstress by B0.1–10 bar on the nearby thrust faults over the inter-seismic phase. Masstransfers induced by surface processes in general, during continuous or short-lived andintense events, represent a prominent mechanism for inter-seismic stress loading offaults near the surface. Such stresses are probably sufficient to trigger shallow seismicity orpromote the rupture of deep continental earthquakes up to the surface.
ISSN: 2041-1723

insu-01087103
https://hal-insu.archives-ouvertes.fr/insu-01087103
https://hal-insu.archives-ouvertes.fr/insu-01087103/document
https://hal-insu.archives-ouvertes.fr/insu-01087103/file/Steer2014_NatureComm_accepted.pdf
DOI : 10.1038/ncomms6564

Éditeur(s) : HAL CCSD Elsevier
Résumé : International audience
We analyze in this study a new set of marine data including 3D local tomography, 1992-2008 relocated earthquakes and two recent multichannel seismic lines to characterize the deformation style in the collision area offshore east Taiwan. We have mapped in detail the Mohos of the converging plates as well as the subduction interface with a resolution never reached before. We show that the sharp continental subduction of the Eurasia plate, beneath the middle part of the Central Range, indents the Philippine Sea plate (PSP) as attested by intra-oceanic slicing and incipient subduction of the PSP beneath the east coast of Taiwan. The westernmost part of the PSP slab is probably experiencing a beginning of break-off as attested by NW-trending en-échelon shear zones beneath the southern slope of the southern Ryukyu arc (SRA). These en-échelon shear zones have a sinistral component favored by the "collision-free" subduction of the PSP north of 24°30′N. The down-faulting of the subduction interface forms ramps along which earthquakes clusterize. Three M7 subduction earthquakes occurred offshore Suao city along these ramps with a recurrence interval of about 40 years: 1920 Mw7.7, 1963 Mw7.2 and 2002 Mw7.1 events. The 1966 Mw6.0-7.5 earthquakes sequence likely outlines a WNW-ESE left-lateral intra-slab shear zone. The SRA upper plate accommodates the complex geometry and deformation of the subducting PSP through seismic deformation. Shallow high velocities fringing the Luzon volcanic arc (LVA) beneath the Longitudinal Valley and north of the southernmost Ryukyu forearc basins are interpreted as relics of the LVA forearc basement squeezed in the collision zone. Based on the accommodation of a large part of the convergence through shortening within the PSP and the subsequent segmentation of the shallow subduction interface, we consider that the nucleation of a Mw ≥ 8 earthquake along the southernmost Ryukyu megathrust is unlikely.
ISSN: 0040-1951

hal-00853365
https://hal.archives-ouvertes.fr/hal-00853365
https://hal.archives-ouvertes.fr/hal-00853365/document
https://hal.archives-ouvertes.fr/hal-00853365/file/publallemandifremer.pdf
DOI : 10.1016/j.tecto.2013.03.020

Éditeur(s) : HAL CCSD Oxford University Press (OUP)
Résumé : International audience
The newly-desribed Centropyge cocosensis has long been considered a variant of the lemonpeel pygmy angelfish C. flavissima because of their overall similarity in color patterns. DiBattista and co-authors [J. Hered. 107 (2016) 647–653] recently contested the validity of C. cocosensis, based on arguments that are challenged here.
ISSN: 0022-1503

ird-01525431
http://hal.ird.fr/ird-01525431
http://hal.ird.fr/ird-01525431/document
http://hal.ird.fr/ird-01525431/file/Letter%20to%20Editor_JHered%20p%20haL.pdf
DOI : 10.1093/jhered/esx008

Éditeur(s) : HAL CCSD Elsevier
Résumé : International audience
An active seismic experiment has been conducted across the southern Ryukyu margin east of Taiwan over the whole trench-arc-backarc system in May 2009. Twenty-four ocean bottom seismometers (OBS) were deployed from the Ryukyu trench to the southern Okinawa trough over the Ryukyu arc and forearc. Wide angle seismic data were recorded by the OBS array while coincident reflection seismic data were acquired using a 6 km long streamer and a 6600 cubic inch seismic airgun array. Results from tomographic inversion of 21091 travel time picks along this line allowed us to image crustal structures of the Ryukyu margin down to a depth of 25 km. The transect has been designed to provide a better seismic velocity structure of the subduction zone in a highly deformed area that has produced an M8 earthquake in 1920. The line crosses a seismic cluster of earthquakes which source mechanisms are still poorly understood. The subducting oceanic crust of the Huatung Basin is about 5-6 km thick. The underlying mantle exhibits low seismic velocities around 7.8 km/s suggesting some hydrothermal alterations or alteration of the upper mantle through faults generated by the flexure of the subducting plate as it enters the subduction. Low velocities, up to 4.5 km/s, associated with the accretionary wedge are well imaged from the trench back to the Nanao forearc. A major result concerns the abrupt termination of the buttress at the rear of the accretionary wedge. Despite the low resolution of the tomographic inversion near the subduction interface, several lines of evidence supporting the presence of a low velocity zone beneath the toe of the forearc buttress could be established. The Moho beneath the Ryukyu non-volcanic arc is located at a depth around 25 km depth.
ISSN: 0040-1951

hal-00795573
https://hal.archives-ouvertes.fr/hal-00795573
DOI : 10.1016/j.tecto.2011.10.010

Éditeur(s) : HAL CCSD Public Library of Science
Résumé : International audience
The cyp19a1 gene that encodes aromatase, the only enzyme permitting conversion of C19 aromatizable androgens into estrogens, is present as a single copy in the genome of most vertebrate species, except in teleosts in which it has been duplicated. This study aimed at investigating the brain expression of a cyp19a1 gene expressed in both gonad and brain of Japanese eel, a basal teleost. By means of immunohistochemistry and in situ hybridization, we show that cyp19a1 is expressed only in radial glial cells of the brain and in pituitary cells. Treatments with salmon pituitary homogenates (female) or human chorionic gonadotrophin (male), known to turn on steroid production in immature eels, strongly stimulated cyp19a1 messenger and protein expression in radial glial cells and pituitary cells. Using double staining studies, we also showed that aromatase-expressing radial glial cells exhibit proliferative activity in both the brain and the pituitary. Altogether, these data indicate that brain and pituitary expression of Japanese eel cyp19a1 exhibits characteristics similar to those reported for the brain specific cyp19a1b gene in teleosts having duplicated cyp19a1 genes. This supports the hypothesis that, despite the fact that eels also underwent the teleost specific genome duplication, they have a single cyp19a1 expressed in both brain and gonad. Such data also suggest that the intriguing features of brain aromatase expression in teleost fishes were not gained after the whole genome duplication and may reflect properties of the cyp19a1 gene of ancestral Actinopterygians.
ISSN: 1932-6203

hal-00877375
https://hal.archives-ouvertes.fr/hal-00877375
https://hal.archives-ouvertes.fr/hal-00877375/document
https://hal.archives-ouvertes.fr/hal-00877375/file/Expressionsur Aromatase-accepted.pdf
DOI : 10.1371/journal.pone.0044750

Éditeur(s) : HAL CCSD Elsevier
Résumé : International audience
The southernmost part of the Ryukyu subduction, where the Philippine Sea Plate is subducting under the Eurasian Plate, is known to be a very seismically active region of transition from a north-dipping subduction along the Ryukyu subduction to an ~ SE-NW collision along the Taiwanese orogenic wedge. In this paper, we will focus on the Ryukyu forearc area close to Taiwan where the deformation is paroxysmal. In order to decipher the nature of the seismic deformation in this region, a three month passive experiment, combining 22 Ocean Bottom Seismometers and 51 onland stations, has been led. Starting from an a-priori heterogeneous model, we have obtained 801 well-located earthquake hypocenters, a precise P-wave tomography model and 14 focal mechanisms. The seismicity along the Ryukyu forearc is mainly located not only in the vicinity of the Interplate Seismogenic Zone (ISZ) but also within both the subducting PSP and the overriding plate. Seismicity within the upper-plate is essentially localized east of Nanao basin where NW-SE extension occurs, and northwest of the Hoping basin where strike-slip dominates. As revealed by both the P-wave velocity structure and the newly derived seismicity, we argue that a sub-vertical step offsetting the subducting PSP around 10 km may support the presence of a trench-parallel tear. The PSP also undergoes extension in its upper part that is probably caused by buckling and slab pull. The P-wave velocity structure reveals three other major features: (1) a continuity between the Central Range and the Ryukyu Arc with a shallower Moho (~ 30 km depth) between ~ 122.3°N and ~ 122.5°N along the Ryukyu Arc, (2) high P-wave velocities along the eastern side of the Central Range and, (3) two bodies with similar high crustal velocities (6.5-7.0 km/s) at 12-18 km depths, embedded within the Ryukyu arc basement, just north of Hoping Basin and north of the Nanao Basin.
ISSN: 0040-1951

hal-00795521
https://hal.archives-ouvertes.fr/hal-00795521
DOI : 10.1016/j.tecto.2012.04.011

Éditeur(s) : HAL CCSD
Résumé : International audience
Geodynamics and Environment in East Asia Symposium (GEEA Meeting)
Hualien, Taiwan

insu-01119754
https://hal-insu.archives-ouvertes.fr/insu-01119754

Éditeur(s) : HAL CCSD
Résumé : International audience
Geodynamics and Environment in East Asia Symposium (GEEA Meeting)
Hualien, Taiwan

insu-01119753
https://hal-insu.archives-ouvertes.fr/insu-01119753

Éditeur(s) : HAL CCSD Cambridge University Press
Résumé : International audience
Genetic variation was examined in two complex cases of Indo-Pacific pygmy angelfishes (genus Centropyge; Pomacanthidae). The lemonpeel pygmy angelfish C. flavissima (Cuvier and Valenciennes) has a geographically disjunct Indian vs. Pacific distribution and the individuals from these two regions differ by their colour patterns. Previous research on C. flavissima has shown mitochondrial introgression from two related species, C. eibli in the eastern Indian Ocean and C. vrolikii in the Pacific Ocean. Using the 16S rDNA and the CO1 gene as phylogeographic markers, we found no mitochondrial haplotypes in common between Indian Ocean C. flavissima and C. eibli, confirming partial genetic isolation, albeit recent. Also, we found substantial genetic differences between Indian and Pacific C. flavissima populations at the nuclear ETS-2 intron locus. The Indian Ocean form of C. flavissima, thus geographically isolated by >2000 km distance from its Pacific Ocean counterpart, is described as a new species, Centropyge cocosensis sp. nov. Centropyge cocosensis sp. nov. differs in appearance from C. flavissima in having a conspicuous blue iris and a fainter, bluish eye ring. We also found that the yellow pygmy angelfish C. heraldi Woods and Schultz consists of two genetically distinct entities, one distributed widely in the northern tropical Indo-West Pacific, the other distributed in the southern Pacific Ocean. The name originally given to the blackfin pygmy angelfish, C. woodheadi Kuiter, is here resurrected to designate the latter.
EISSN: 1755-2672

hal-01340521
https://hal-univ-perp.archives-ouvertes.fr/hal-01340521
DOI : 10.1007/s12526-016-0509-y

Éditeur(s) : HAL CCSD Elsevier
Résumé : International audience
The Iranian plateau is a flat ~ 1.5–2 km high plateau thought to result from the collision between the Arabian and Eurasian plates since ~ 30 ± 5 Ma, and may represent a young analogue to the so far better studied Tibetan plateau. In order to constrain the exhumation history of the internal Zagros and of the Iranian plateau, we herein present apatite (U-Th)/He (AHe) and apatite (AFT) and zircon fission-track (ZFT) data on plutonic rocks from the Sanandaj–Sirjan Zone (SSZ), Urumieh–Dokhtar magmatic arc (UDMA), Central Iran and Kopet Dagh. Thermochronologic data show that the SSZ was exhumed early in the collision process (essentially before 25–20 Ma), with a likely acceleration of cooling during the late Eocene, from 0.04 to 0.3 mm/year. Results suggest that cooling of the internal Zagros migrated from the SSZ to the UDMA during a more mature stage of the continental collision, after ~ 17 Ma (i.e., coeval with the outward propagation of deformation and topography fronts in the external Zagros). Constant exhumation rates in the UDMA (~ 0.3 mm/year) suggest that no significant variation of erosion rates occurred since the onset of continental collision. In Central Iran, the overlap of ZFT, AFT and AHe ages from gneissic samples points to rapid cooling during the late Eocene (~ 42 °C/Myr), which is consistent with previous reports on the formation of Eocene metamorphic core-complexes.
ISSN: 0024-4937

hal-01115691
https://hal.archives-ouvertes.fr/hal-01115691
DOI : 10.1016/j.lithos.2014.07.021

Éditeur(s) : HAL CCSD Elsevier
Résumé : International audience
Orogenic wedges locally present chaotic tectonostratigraphic units that contain exotic blocks of various size, origin, age and lithology, embedded in a sedimentary matrix. The occurrence of ophiolitic blocks, sometimes huge, in such “mélanges” raises questions on (i) the mechanisms responsible for the incorporation of oceanic basement rocks into an accretionary wedge and (ii) the mechanisms allowing exhumation and redeposition of these exotic elements in “mélanges” during wedge growth.To address these questions, we present the results of a series of analogue experiments performed to characterize the processes and parameters responsible for accretion, exhumation and tectonosedimentary reworking of oceanic basement lithospheric fragments in an accretionary wedge.The experimental setup is designed to simulate the interaction between tectonics, erosion and sedimentation. Different configurations are applied to study the impact of various parameters, such as irregular oceanic floor due to structural inheritance, or the presence of layers with contrasted rheology that can affect deformation partitioning in the wedge (frontal accretion vs basal accretion) influencing its growth. Image correlation technique allows extracting instantaneous velocity field, and tracking of passive particles. By retrieving the particle paths determined from models, the pressure-temperature path of mélange units or elementary blocks can be discussed. The experimental results are then compared with observations from ophiolite-bearing mélanges in Taiwan (Lichi and Kenting mélanges) and Raman spectroscopy of carbonaceous material (RSCM) Thermometry data on rocks from the northern Apennines (Casanova mélange). A geological scenario is proposed following basic observations. The tectonic evolution of the retroside of doubly vergent accretionary wedges is mainly controlled by backthrusting and backfolding. The retro wedge is characterized by steep slopes that are prone to gravitational instabilities. It triggers submarine landslides inducing huge mass transfers. This erosion combined with backthrusting could favour exhumation of the ophiolitic fragments formerly accreted at the base of the wedge along the rough seafloor-sediments interface. Such an exhumed material can be reworked and deposited as debris- flows in proximal basins located at the foot of the retrowedge slope forming a tectono-sedimentary mélange. These syntectonic basins are continuously deformed and involved in prograding backthrusting-induced deformation.
ISSN: 0264-3707

hal-01395127
https://hal.archives-ouvertes.fr/hal-01395127
DOI : 10.1016/j.jog.2016.05.008

Éditeur(s) : HAL CCSD Georg Thieme Verlag
Résumé : International audience
Oil from the nuts of Calophyllum inophyllum, locally called "Tamanu oil" in French Polynesia, was traditionally used for wound healing and to cure various skin problems and ailments. The skin-active effect of "Tamanu oil emulsion" was investigated on human skin cells (keratinocytes and dermal fibroblasts) and showed cell proliferation, glycosaminoglycan and collagen production, and wound healing activity. Transcriptomic analysis of the treated cells revealed gene expression modulation including genes involved in the metabolic process implied in O-glycan biosynthesis, cell adhesion, and cell proliferation. The presence of neoflavonoids as bioactive constituents in Tamanu oil emulsion may contribute to these biological activities. Altogether, consistent data related to targeted histological and cellular functions brought new highlights on the mechanisms involved in these biological processes induced by Tamanu oil effects in skin cells.
ISSN: 0032-0943

hal-01592246
https://hal.archives-ouvertes.fr/hal-01592246
DOI : 10.1055/s-0042-108205

Éditeur(s) : HAL CCSD Elsevier
Résumé : International audience
The Larsemann Hills area is part of a reworked early Neoproterozoic metamorphic terrain in southwestern Prydz Bay, East Antarctica. Ultramafic and mafic granulites, whose origins remain controversial, occur as lenses, boudins or layered bodies within the para- and ortho-gneiss in the region. The ultramafic and mafic granulites show spinel-olivine-bearing and two-pyroxene-bearing mineral assemblages recrystallized at 860–900 °C. Their bulk rock analyses indicate an origin as igneous cumulates, with high Mg# (molar MgO/(MgO + FeO)) from 0.73 to 0.84 for ultramafic granulite and from 0.46 to 0.78 for mostly mafic granulite as well as high Cr and Ni contents ([Cr] and [Ni] up to 1826 ppm and 1400 ppm respectively for ultramafic granulite and [Cr] of 1460 ppm for mafic granulite). Trace element patterns show pronounced negative Nb anomalies, suggesting a subduction-related tectonic setting for their precursors, consistent with derivation from arc basalts, also suggested by low TiO2 and K-enrichment in mafic granulites. The ultramafic to mafic granulites may have been formed in a subduction-related continental back-arc basin environment simultaneously with peak metamorphism associated with arc-continent collision during the early Neoproterozoic (990–900 Ma) Rayner orogeny.
ISSN: 0743-9547

hal-01622589
https://hal.archives-ouvertes.fr/hal-01622589
DOI : 10.1016/j.jseaes.2017.06.012

Éditeur(s) : HAL CCSD Wiley-Blackwell
Résumé : International audience
We explore the tectono-magmatic processes in the western West Philippine Basin, Philippine Sea Plate, using bathymetric data acquired in 2003 and 2004. The northwestern part of the basin formed through a series of northwestward propagating rifts. We identify at least five sequences of propagating rifts, probably triggered by mantle flow away from the mantle thermal anomaly that is responsible for the origin of the Benham and Urdenata plateaus. Gravitational forces caused by along-axis topographic gradient and a ∼30° ridge reorientation appear to also be driving the rift propagations. The along-axis mantle flow appears to be reduced and deflected along the Luzon-Okinawa fracture zone, because the spreading system remained stable west of this major fault zone. North-east of the Benham plateau, a left-lateral fracture zone has turned into a NE–SW-trending spreading axis. As a result, a microplate developed at the triple junction.
ISSN: 0954-4879

insu-00304360
https://hal-insu.archives-ouvertes.fr/insu-00304360
DOI : 10.1111/j.1365-3121.2008.00824.x