Éditeur(s) : HAL CCSD
Résumé : International audience
STUDY QUESTION: Do freezing and in vitro culture procedures enhance the expression of proteins involved in apoptotic or autophagic pathways in murine pre-pubertal testicular tissue? SUMMARY ANSWER: IVM strongly modified apoptosis- and autophagy-related relative protein levels in mice testicular tissue whereas the impact of cryopreservation procedures was minimal at the end of the culture. WHAT IS KNOWN ALREADY: In vitro spermatogenesis remains a challenging technical issue as it imposes to find a very close balance between survival and death of germ cell natural precursors (i.e. gonocytes and spermatogonia), which will eventually undergo a complete spermatogenesis close to in vivo conditions. The establishment of efficient culture conditions coupled with suitable cryopreservation procedures (e.g. controlled slow freezing [CSF] and solid surface vitrification [SSV]) of pre-pubertal testicular tissue is a crucial step in the fields of fertility preservation and restoration to improve the spermatic yield obtained in vitro. STUDY DESIGN, SIZE, DURATION: Here, we study cryopreservation procedures (i.e. CSF or SSV) and the impact of culture media compositions. A first set of 66 mouse pre-pubertal testes were directly cultured during 30, 36, 38 and 60 days (D) from 2.5 to 6.5-day-old CD-1 mice to evaluate the impact of time-aspect of culture and to endorse the reverse phase protein microarrays (RPPM) technique as an adapted experimental tool for the field of in vitro spermatogenesis. Ninety others fresh, slow-frozen and vitrified pre-pubertal testes were cultured during 30 days for the principal study to evaluate the impact of cryopreservation procedures before and after culture. Thirty-four testes dissected from 2.5, 6.5, 36.5, 40.5, 42.5 and 62.5 days postpartum (dpp) mice, corresponding to the time frames of spermatogenesis orchestrated in vitro, were used as in vivo controls. PARTICIPANTS/MATERIALS, SETTING, METHODS: After in vitro culture, testicular tissue samples originated from 2.5 or 6.5-day-old CD-1 male mice were analyzed using RPPM. This targeted proteomic technique allowed us to assess the expression level of 29 apoptosis- and autophagy-related factors by normalizing blank-corrected signal values. In addition, morphological analyses (e.g. HES, PAS, TRA98 and CREM) and DNA fragmentation in intra-tubular cells (i.e. terminal deoxynucleotidyl transferase dUTP nick end labeling; TUNEL) were assessed for the distinct experimental conditions tested as well as for in vivo control mouse testes. MAIN RESULTS AND THE ROLE OF CHANCE: A validation of the RPPM procedure in the field of in vitro spermatogenesis was completed with assay and array robustness before a principal study concerning the evaluation of the impact of in vitro culture and cryopreservation procedures. The proportion of elongated spermatids and the total cell number per seminiferous tubule tended to be very different between the in vivo and in vitro conditions (P < 0.05), suggesting the presence of a beneficial regulation on the first spermatogenesis wave by intrinsic apoptosis (Caspase₉) and autophagy (Atg5) factors (P < 0.0003 and r2 = 0.74). Concerning the impact of culture media compositions, a basic medium (BM) composed of αMEM plus 10% KnockOut™ serum replacement and gentamicin supplemented with retinol (Rol) and vitamin E (Vit. E) was selected as the best culture medium for fresh 6.5 dpp tissue cultured during 30D with 27.7 ± 8.10% of seminiferous tubules containing elongated spermatids. Concerning the impact of cryopreservation procedures, SSV did not have any impact on the morphological parameters evaluated after culture in comparison to fresh tissue (FT) controls. The proportion of tubules with elongated spermatids on testicular explants cultured with BMRol+Vit. E was not different between SSV (6.6 ± 1.6%) and CSF (5.3 ± 1.9%); however, round spermatids were observed more frequently for SSV (19 ± 6.2%) than CSF (3.3 ± 1.9%, P = 0.0317). Even if the proportion of TUNEL-positive cells for BMRol+Vit. E was higher at D30 after SSV (4.12 ± 0.26%) than CSF (1.86 ± 0.12%, P = 0.0022) and FT (2.69 ± 0.33%, P = 0.0108), the DNA damages observed at the end of the culture (i.e. D30) were similar to respective 6.5 dpp controls. In addition, the relative protein level expression ratio of an apoptotic factor, the phosphorylated FADD on Fas, was reduced by 64-fold in vitrified testes cultured with BMRol+Vit. E. Furthermore, we found in this study that the StemPro®-34 SFM culture medium supplemented with growth factors (e.g. EGF, bFGF, GDNF and LIF) prevented the differentiation of spermatogonial stem cells in favor of a significant proliferation with a better architectural pattern than in vivo 6.5 dpp controls with an increase of seminiferous tubules area for FT (P = 0.0357) and CSF (P = 0.0317). LIMITATIONS REASONS FOR CAUTION: Despite our promising results, the evaluation of apoptotic- and autophagic-related proteins was studied for a limited amount of proteins and on global testicular tissue. WIDER IMPLICATIONS OF THE FINDINGS: The data presented herein will help to improve apoptotic and autophagic understanding during the first spermatogenic wave. Moreover, our findings illustrate for the first time that, using finely-tuned experimental conditions, a testicular in vitro culture combined with proteomic technologies may significantly facilitate the study of cryopreservation procedures and in vitro culture evaluations. This study may also contribute to improve work on testicular tissues from pre-pubertal and adolescent cancer survivors. STUDY FUNDING/COMPETING INTEREST(S): This study was supported by a Ph.D. grant from the Rouen Normandie Université and a financial support from 'la Ligue nationale contre le cancer' (both awarded to L.D.), funding from Institute for Research and Innovation in Biomedicine (IRIB), Agence de la Biomédecine, and co-supported by European Union and Région Normandie. Europe gets involved in Normandie with European Regional Development Fund (ERDF). The authors declare that there is no conflict of interest.
Molecular Human Reproduction

hal-01647081
https://hal-univ-rennes1.archives-ouvertes.fr/hal-01647081
DOI : 10.1093/molehr/gax054
PUBMED : 29040674

Éditeur(s) : HAL CCSD
Résumé : International audience
A prospective study on randomized patients was conducted to determine how morphokinetic parameters are altered in embryos grown in sequential versus global culture media. Eleven morphokinetic parameters of 160 single embryos transferred were analyzed by time lapse imaging involving two University-affiliated in vitro fertilization (IVF) centers. We found that the fading of the two pronuclei occurred earlier in global (22.56±2.15 hpi) versus sequential media (23.63±2.71 hpi; p=0.0297). Likewise, the first cleavage started earlier at 24.52±2.33 hpi vs 25.76±2.95 hpi (p=0.0158). Also, the first cytokinesis was shorter in global medium, lasting 18±10.2 minutes in global versus 36±37.8 minutes in sequential culture medium (p <0.0001). We also observed a significant shortening in the duration of the 2-cell stage in sequential medium: 10.64 h±2.75 versus 11.66 h±1.11 in global medium (p=0.0225) which suggested a faster progression of the embryos through their first mitotic cell cycle. In conclusion, morphokinetic analysis of human embryos by Time lapse imaging reveals significant differences in five kinetic variables according to culture medium. Our study highlights the need to adapt morphokinetic analysis accordingly to the type of media used to best support human early embryo development.
Annales de Biologie Clinique

hal-01579406
https://hal-univ-rennes1.archives-ouvertes.fr/hal-01579406
DOI : 10.1684/abc.2017.1259
PUBMED : 28751285

Éditeur(s) : Université des Antilles Études caribéennes
Résumé : En adoptant les Objectifs du Millénaire pour le Développement (OMD) des Nations Unies en 2000, la communauté internationale a placé la réduction de la pauvreté en tête des priorités de l’agenda politique mondial. Par-là, la communauté internationale n’a pas seulement placé la lutte contre la pauvreté sous les projecteurs des medias, elle l’a également rendu difficile à remettre en cause. Qui pourrait décemment s’opposer à la réduction de la pauvreté dans le monde ?Si l’on veut cibler les « pauvres » dans la mise en œuvre d’une politique, il faut d’abord pouvoir les reconnaître. Or ceux-ci sont définis par les institutions internationales en fonction d’un seuil de pauvreté universel établi à moins de 1 ou de 2 $ par jour ! La pauvreté n’est pas réductible à une question de richesse monétaire quotidienne et l’on sait qu’elle varie en fonction de nombreux critères géographiques, culturels, sociologiques, psychologiques,…Cette conception réductrice de la pauvreté se traduit par une catégorisation parfois arbitraire et stigmatisante, qui marque du sceau de la pauvreté des personnes qui ne se considèrent pas forcément comme telles, avec pour conséquence de se tromper potentiellement de cible, et dans certains cas, de contribuer à un passage de la pauvreté à la misère.Nous proposons ici une déconstruction de la conception utilitariste de la pauvreté et de la richesse sur laquelle repose la plupart des politiques mises en œuvre s’inscrivant dans l’objectif officiel de « réduction de la pauvreté ». Cette déconstruction nous permet non seulement de faire ressortir la pluralité des pauvretés mais également de réinscrire l’objectif de « réduction de La pauvreté » dans les enjeux de pouvoir sur la qualification de « pauvre » et sur les conceptions de la richesse qui peuvent légitimer ce pouvoir.
By adopting the Millennium Development Goals (MDGs) in the United Nations in 2000, the international community has made poverty reduction a top priority on the global political agenda. In doing this, the international community has put poverty reduction in the media spotlight, which has also made it more difficult to challenge. Yet who would decently oppose the reduction of global poverty?If we want to target the poor in policy implementation, it is first necessary to be able to recognize them. But these people are defined by international institutions based on a universal poverty threshold established at less than $1 - $2 per day. Poverty depends on a multitude of geographic, cultural, sociological, psychological and other criteria. As such, it cannot be reduced merely to a question of daily monetary wealth.This reductionist approach to poverty leads to adopt categorizations which are sometimes arbitrary and stigmatizing because they label people who do not necessarily consider themselves poverty-stricken. This can result in policy which targets the wrong people, and in some cases contributes to a transition from poverty to misery.In this paper we propose a deconstruction of the utilitarian approach to poverty and wealth on which most “poverty reduction” policies are based. This deconstruction allows us not only to emphasize the diversity of types of poverty, but also to reinforce the objective of "reducing poverty" within power relations in regards to the significance of poverty and the concepts of wealth that can legitimize that power.

Droits : info:eu-repo/semantics/openAccess
urn:doi:10.4000/etudescaribeennes.6490
http://journals.openedition.org/etudescaribeennes/6490

Éditeur(s) : Université des Antilles Études caribéennes
Résumé : The aim of this paper is to study Moroccan music festivals against the existing theories on globalization, while coming up with new concepts aimed at overcoming the problems often facing experts in popular culture and media studies who feel that globalization thesis lapses into fatal gestures of leveling, reductionism, and totalitarianism, especially when it tries to account for current discussions related to the circulation of artifacts and cultural texts. The general consensus has it that modern technologies have hugely altered the meaning and revolutionized the traditional functions attached to art and cultural industries. The grotesque circulation of western cultural artifacts is justifiably judged to be many times agonizing for theorists in cultural studies, linguistics and political science, etc who want to be sure that the unequal transmissions of products across the world will not harm local cultural, linguistic and economic capital of the less dominant other. Moroccan music festivals for instance are seen to be increasingly governed by a complex whirlpool of the far-too-melodramatic implications of today’s world global connections. In view of this, this paper, which borrows from data collected during between 2010 and 2011, will examine cultural industries, music festivals in general and the Essaouira festival in particular, in relation to world flows, while holding the argument that the appropriation of western artifacts locally has always been part of a not-so-novel process of mobility of what we call world cultural nomadictates (nomadic dictates). The paper gives a detailed definition of this concept and develops other new concepts (e.g. recurents, exclusives) related to the «glocal» face of culture and art in Morocco in an attempt to find an escape route outside the impasses of the globalization/cultural imperialism theses surrounding the study of cultural industries. To drive this idea home, we will open up the discussion at hand onto existing controversies around notions of place, authenticity, urbanism, tourism and consumption.
L'objectif de cette recherche est d’étudier les festivals de musique marocains en portant un regard critique sur les théories existantes de la mondialisation et en proposant de nouveaux concepts afin de surmonter les problèmes auxquels les théoriciens de la culture populaire et des médias se sont souvent confrontés. Selon eux, le concept de mondialisation sombre dans le réductionnisme et le totalitarisme, surtout lorsqu’il s’agit de la circulation des objets et des textes culturels. Un consensus général semble s’être développé autour de l’idée selon laquelle les technologies modernes ont considérablement altéré et révolutionné le sens et les fonctions traditionnelles de l'art et des industries culturelles. L'insolite diffusion des objets culturels occidentaux est à juste titre source d’angoisse pour les théoriciens des études culturelles, de la linguistique, et des sciences politiques qui veulent s'assurer que la transmission inégale des produits dans le monde ne nuira pas au capital local, culturel, linguistique et économique de l'autre (le moins dominant). Les festivals de musique marocains, par exemple, sont perçus comme étant de plus en plus tributaires des vicissitudes de la mondialisation actuelle. Dans cette perspective, cette recherche, s'inspire de données recueillies au cours de deux années de travail de terrain (2010/2011), et se penche sur les flux mondiaux de produits culturels qui mettent en interface les industries culturelles, les festivals de musique en général et celui d'Essaouira en particulier. Elle maintient l'idée selon laquelle l'appropriation des objets occidentaux par les communautés locales a toujours fait partie d'un processus de mobilité, qui n'est pas si récent, de ce que nous appelons les ‘nomadictâtes’ culturels du monde (dictâtes nomades). En explicitant la définition de ce concept, cette recherche développe d'autres concepts liés à la notion de glocalité de la culture et de l'art au Maroc. Enfin, elle suggère aussi une issue à l’impasse des théories de la mondialisation traditionnelles et celle de l’impérialisme culturel en particulier dans lesquelles l’étude des industries culturelles semble s’être emprisonnée. Pour ce faire, nous exposons le débat actuel sur les controverses autour des notions de lieu, d'authenticité, d'urbanisme, de tourisme, et de consommation.
Maroc

Droits : info:eu-repo/semantics/openAccess
urn:doi:10.4000/etudescaribeennes.5993
http://journals.openedition.org/etudescaribeennes/5993