Éditeur(s) : HAL CCSD American Society of Hematology
Résumé : International audience
ISSN: 0006-4971

hal-01117665
https://hal-univ-rennes1.archives-ouvertes.fr/hal-01117665

Éditeur(s) : HAL CCSD American Society for Microbiology
Résumé : International audience
A number of men receiving prolonged suppressive HAART still shed HIV in semen. To investigate whether this seminal shedding may be due to poor drug penetration and/or viral production by long-lived cells within male genital tissues, we analyzed semen and reproductive tissues from macaques chronically infected with SIVmac251 submitted to HAART for 4 months, intensified over the last seven weeks with an integrase inhibitor. We showed that a subset of treated animals continued shedding SIV in semen despite efficient HAART. This shedding was not associated with low antiretroviral drug concentrations in semen, or in testis, epididymis, seminal vesicles and prostate. HAART had no significant impact on SIV RNA in the urethra, whereas it drastically reduced SIV RNA levels in the prostate and vas deferens, and to a lesser extent in the epididymis and seminal vesicle. The only detectable SIV RNA positive cells within the male genital tract after HAART were urethral macrophages. SIV DNA levels in genital tissues were not decreased by HAART, suggesting the presence throughout the male genital tract of non-productively infected cells. In conclusion, our results demonstrate that 4 months of HAART induced variable and limited control of viral infection in the male reproductive organs, particularly the urethra, and suggest that infected long-lived cells in the male genital tract may be involved in persistent seminal shedding during HAART. These results pave the way for further investigations of male genital organs infection in long term treated infected individuals. A substantial subset of men receiving prolonged suppressive HAART in the blood still harbor HIV in semen, and cases of sexual transmission have been reported. To understand the origin of this persistence, we analyzed the semen and male reproductive tissues from SIV-infected macaques treated by HAART. We demonstrated that persistent seminal shedding was not linked to poor drug penetration in semen or semen-producing prostate, seminal vesicle, epididymis and testis. We revealed that HAART decreased SIV RNA to various extents in all male genital organs, with the exception of the urethra, in which SIV RNA+ macrophages were observed despite HAART. Importantly also, HAART did not impact SIV DNA levels in the male genital organs. These results suggest that infection of male genital organs, and particularly the urethra, could be involved in the release of virus in semen during HAART.
ISSN: 0022-538X

hal-01139816
https://hal-univ-rennes1.archives-ouvertes.fr/hal-01139816
https://hal-univ-rennes1.archives-ouvertes.fr/hal-01139816/document
https://hal-univ-rennes1.archives-ouvertes.fr/hal-01139816/file/Detection%20of%20SIV%20in%20Semen%2C%20Urethra%2C%20and%20Male%20Reproductive%20Organs%20during%20Efficient%20Highly%20Active%20Antiretroviral%20Therapy.pdf
PUBMEDCENTRAL : PMC4442442

Éditeur(s) : HAL CCSD Oxford University Press (OUP): Policy B
Résumé : International audience
AIMS: Indications for surgery in acute infective endocarditis (IE) are detailed in guidelines, but their application is not well known. We analysed the agreement between the patient's attending physicians and European Society of Cardiology guidelines regarding indications for surgery. We also assessed whether surgery was performed in patients who had an indication. METHODS AND RESULTS: From the 2008 prospective population-based French survey on IE, 303 patients with definite left-sided native IE were identified. For each case, we prospectively recorded (i) indication for surgery according to the attending physicians and (ii) indication for surgery according to guidelines. Surgery was indicated in 194 (65%) patients according to attending physicians and in 221 (73%) according to guidelines, while 139 (46%) underwent surgery. Agreement was moderate between attending physicians and guidelines (kappa 0.41-0.59) and between indication according to guidelines and the performance of surgery (kappa 0.38). Of the 90 (30%) patients not operated despite indication, contraindication to surgery was reported by the attending physicians in 42 (47%), and indication was not identified in 48 (53%). One-year survival was 76% in patients with indication and surgery performed (n = 131), 69% in patients without indication and no surgery (n = 74), 56% in patients with identified indication and contraindication to surgery (n = 42), and 60% in patients with no identified indication (n = 48; P = 0.059). CONCLUSION: Cardiac surgery during acute IE was recommended in almost three out of four patients, although fewer than half were actually operated. Indication was not acknowledged by the attending physicians in one out of six patients
ISSN: 0195-668X

hal-01255874
https://hal-univ-rennes1.archives-ouvertes.fr/hal-01255874
DOI : 10.1093/eurheartj/ehv650
PUBMED : 26685134

Éditeur(s) : HAL CCSD CGMW/SGF
Seismic Atlas of the Messian Salinity Crisis markers in the Mediterranean and Black Seas

ISBN : 2-85363-097-8
hal-00817416
https://hal.archives-ouvertes.fr/hal-00817416

Éditeur(s) : HAL CCSD Copernicus Publications
Résumé : Drilling an ultra-deep hole in an intact portion of oceanic lithosphere, through the crust to the Mohorovičić discontinuity (the ‘Moho'), and into the uppermost mantle is along-standing ambition of scientific ocean drilling (Bascom,1961; Shor, 1985; Ildefonse et al., 2007). It remains essential to answer fundamental questions about the dynamics of the Earth and global elemental cycles. The global system of mid-ocean ridges and the new oceanic lithosphere formed at these spreading centers are the principal pathways for energy and mass exchange between the Earth's interior, hydrosphere, and biosphere. Bio-geochemical reactions between the oceans and oceanic crust continue from ridge to subduction zone, and the physical and chemical changes to the ocean lithosphere provide inventories of these thermal, chemical, and biological exchanges.
ISSN: 1816-8957

hal-00564847
https://hal.archives-ouvertes.fr/hal-00564847
DOI : 10.2204/iodp.sd.10.07.2010

Éditeur(s) : HAL CCSD
Résumé : National audience
Journées IODP France
Paris, France

insu-01133131
https://hal-insu.archives-ouvertes.fr/insu-01133131

Éditeur(s) : HAL CCSD Elsevier
Résumé : Fire-history reconstructions inferred from sedimentary charcoal records are based on measuring sieved charcoal fragment area, estimating fragment volume, or counting fragments. Similar fire histories are reconstructed from these three approaches for boreal lake sediment cores, using locally defined thresholds. Here, we test the same approach for a montane Mediterranean lake in which taphonomical processes might differ from boreal lakes through fragmentation of charcoal particles. The Mediterranean charcoal series are characterized by highly variable charcoal accumulation rates. Results there indicate that the three proxies do not provide comparable fire histories. The differences are attributable to charcoal fragmentation. This could be linked to fire type (crown or surface fires) or taphonomical processes, including charcoal transportation in the catchment area or in the sediment. The lack of correlation between the concentration of charcoal and of mineral matter suggests that fragmentation is not linked to erosion. Reconstructions based on charcoal area are more robust and stable than those based on fragment counts. Area-based reconstructions should therefore be used instead of the particle-counting method when fragmentation may influence the fragment abundance.
ISSN: 0033-5894

hal-00903607
https://hal.archives-ouvertes.fr/hal-00903607
DOI : 10.1016/j.yqres.2013.01.003

Éditeur(s) : HAL CCSD IOP Publishing
Résumé : The Bureau International des Poids et Mesures (BIPM), Sevres, France, hosted the 7th International Comparison of Absolute Gravimeters (ICAG) and the associated Relative Gravity Campaign (RGC) from August to September 2005.;ICAG 2005 was prepared and performed as a metrological pilot study, which aimed:;(1) To determine the gravity comparison reference values;;(2) To determine the offsets of the absolute gravimeters; and;(3) As a pilot study to accumulate experience for the CIPM Key Comparisons.;This document presents a complete and extensive review of the technical protocol and data processing procedures. The 1st ICAG-RGC comparison was held at the BIPM in 1980-1981 and since then meetings have been organized every 4 years.;In this paper, we present an overview of how the meeting was organized, the conditions of BIPM gravimetric sites, technical specifications, data processing strategy and an analysis of the final results. This 7th ICAG final report supersedes all previously published reports.;Readings were obtained from participating instruments, 19 absolute gravimeters and 15 relative gravimeters. Precise levelling measurements were carried out and all measurements were performed on the BIPM micro-gravity network which was specifically designed for the comparison.
ISSN: 0026-1394

hal-00644529
https://hal.archives-ouvertes.fr/hal-00644529
DOI : 10.1088/0026-1394/48/5/003

Éditeur(s) : HAL CCSD
Résumé : International audience
The Transbaikal region extends over several hundreds of kilometres east of the Baikal Rift System. It is characterized by a number of sub-parallel Mesozoic grabens or half grabens generally filled with late Jurassic to Early Cretaceous clastic sediments interbedded with coal layers (1). Similar basins occur on an even larger area spanning from the Transbaikal region down to Korea implying a large-scale extensional process affecting most of the Amuria plate during the Mesozoic. In the Transbaikal region, the normal faults controlling the edges of the Mesozoic basins are generally superimposed to Palaeozoic ductile shear zones implying a strong localisation of the extensional deformation on inherited structures. Recent studies, associated to our own fieldwork demonstrated that some of the faults were again activated (2), still as extensional faults, during the Tertiary or Quaternary, and that some of them are presently active. The closure of the Mongol-Okhotsk ocean separating the Siberian plate from the Amurian block during the Mesozoic corresponds to a major event in the growth process of the East Asian continent. The oceanic suture zone is situated on the southern edge of the Transbaikal region and its roughly SW-NE direction is parallel to the basins (3). The timing of the closure of the Mongol-Okhotsk ocean is still highly debated: while sedimentological and tectonic data suggest that the oceanic closure and the following collision occurred in early Middle Jurassic (4), paleomagnetic studies advocate for a Early Cretaceous collision (5). Furthermore, several other questions remain on the localization, the size and the fate of the relief that most probably formed during the collision between the Amuria block and the Siberian craton. In order to answer those questions we used low temperature thermochronology data associated to tectonic, sedimentology and palinology to investigate the evolution of the Transbaikal grabens from Mesozoic to Present. Tectonic and thermochronology data provide evidences of exhumation and erosion along the eastern edge of the Siberian craton during the Middle Jurassic as well as a potential continuum of deformation between the Mesozoic extension and the initiation of the Baikal Rift System (6). Sedimentology and palinology reveals that the sediments deposited in the Transbaikal basin did not registered large-scale compressive deformation during or after their Late Jurassic - Early Cretaceous deposition and that they do not correspond to the dismantling of a strong compressive relief.
Geophysical Research Abstracts
Vienne, Austria

insu-00810487
https://hal-insu.archives-ouvertes.fr/insu-00810487

Éditeur(s) : HAL CCSD Hindawi Publishing Corporation
Résumé : International audience
This paper is concerned with the study of a penalization-gradient algorithmfor solving variational inequalities, namely, find x ∈ C such that Ax, y − x ≥ 0 for all y ∈ C, where A : H → H is a single-valued operator, C is a closed convex set of a real Hilbert space H. Given Ψ : H → ∪ { ∞} which acts as a penalization function with respect to the constraint x ∈ C, and a penalization parameter βk, we consider an algorithm which alternates a proximal step with respect to ∂Ψ and a gradient step with respect to A and reads as xk I λkβk∂Ψ −1 xk−1 − λkAxk−1 . Under mild hypotheses, we obtain weak convergence for an inverse strongly monotone operator and strong convergence for a Lipschitz continuous and strongly monotone operator. Applications to hierarchical minimization and fixed-point problems are also given and the multivalued case is reached by replacing themultivalued operator by its Yosida approximatewhich is always Lipschitz continuous.
ISSN: 0161-1712

hal-00776654
https://hal.univ-antilles.fr/hal-00776654
https://hal.univ-antilles.fr/hal-00776654/document
https://hal.univ-antilles.fr/hal-00776654/file/305856.pdf
DOI : 10.1155/2011/305856

Éditeur(s) : HAL CCSD Bureau de recherches géologiques et minières
Résumé : National audience
Actuellement, une large part de la communauté des Sciences de la Terre étudie les couplages entre enveloppes externes et enveloppes internes de la planète car ils exercent une influence immédiate et sensible sur notre environnement
ISSN: 0292-8477

hal-00795518
https://hal.archives-ouvertes.fr/hal-00795518

Éditeur(s) : HAL CCSD AGU and the Geochemical Society
Résumé : International audience
Analysis of short-term deformation along the southern part of Dead Sea Fault (DSF) provides a systematic view of kinematics this part of the continental transform. The southern DSF consists of two principal segments: the Wadi Araba and Jordan Valley faults. In addition to other regional continuous GPS data, this study uses new data from 25 survey sites and 4 continuous GPS stations in Jordan for improved near-field observations. Resulting velocities are reported with 1-sigma uncertainties ranging from 0.4-1.0 mm/yr. Application of elastic dislocation models yields estimates of slip rates for Wadi Araba and Jordan Valley faults are 4.9 +/- 0.4 mm/yr and 4.7 +/- 0.4 mm/yr, respectively. Modeling also suggests different depths of effective fault locking with 15 +/- 5 km and 8 +/- 5 km for the Wadi Araba and Jordan Valley faults, respectively. These slip rates are generally consistent with the upper end of the range of slip rates estimated from late Quaternary geology. Spatial variations in effective fault locking generally correspond with a heterogeneous mantle lithosphere. A similar observation can be observed along the southern San Andreas Fault, and this may reflect the influence of heterogeneity in the uppermost mantle on crustal faulting processes.
ISSN: 1525-2027

hal-00689031
https://hal.archives-ouvertes.fr/hal-00689031
DOI : 10.1029/2011GC003736

Éditeur(s) : HAL CCSD Taylor & Francis
Résumé : International audience
To analyze the characteristics and outcome of infective endocarditis (IE) according to the time interval between IE first symptoms and diagnosis.
Among the IE cases of a French population-based epidemiological survey, patients having early-diagnosed IE (diagnosis of IE within 1 month of first symptoms) were compared with those having late-diagnosed IE (diagnosis of IE more than 1 month after first symptoms).
Among the 486 definite-IE, 124 (25%) had late-diagnosed IE whereas others had early-diagnosed IE. Early-diagnosed IE were independently associated with female gender (OR = 1.8; 95% CI [1.0-3.0]), prosthetic valve (OR= 2.6; 95% CI [1.4-5.0]) and staphylococci as causative pathogen (OR = 3.7; 95% CI [2.2-6.2]). Cardiac surgery theoretical indication rates were not different between early and late-diagnosed IE (56.3% vs 58.9%), whereas valve surgery performance was lower in early-diagnosed IE (41% vs 53%; p = .03). In-hospital mortality rates were higher in early-diagnosed IE than in late-diagnosed IE (25.1% vs 16.1%; p < .001).
The time interval between IE first symptoms and diagnosis is closely related to the IE clinical presentation, patient characteristics and causative microorganism. Better prognosis reported in late-diagnosed IE may be related to a higher rate of valvular surgery. KEY MESSAGES Infective endocarditis, which time interval between first symptoms and diagnosis was less than one month, were mainly due to Staphylococcus aureus in France. Staphylococcus aureus infective endocarditis were associated with septic shock, transient ischemic attack or stroke and higher mortality rates than infective endocarditis due to other bacteria or infective endocarditis, which time interval between first symptoms and diagnosis was more than one month. Infective endocarditis, which time interval between first symptoms and diagnosis was more than one month, were accounting for one quarter of all infective endocarditis in our study and were associated with vertebral osteomyelitis and a higher rate of cardiac surgery performed for hemodynamic indication than other infective endocarditis.
ISSN: 0785-3890

hal-01509563
https://hal.archives-ouvertes.fr/hal-01509563
PUBMED : 27607562

Éditeur(s) : HAL CCSD American Geophysical Union - AGU
Résumé : without
InterRidge News

hal-00464150
https://hal.archives-ouvertes.fr/hal-00464150

Éditeur(s) : HAL CCSD Nature Publishing Group
Résumé : International audience
DNA cytosine methylation is a widely conserved epigenetic mark in eukaryotes that appears to have critical roles in the regulation of genome structure and transcription. Genome-wide methylation maps have so far only been established from the supergroups Archaeplastida and Unikont. Here we report the first whole-genome methylome from a stramenopile, the marine model diatom Phaeodactylum tricornutum. Around 6% of the genome is intermittently methylated in a mosaic pattern. We find extensive methylation in transposable elements. We also detect methylation in over 320 genes. Extensive gene methylation correlates strongly with transcriptional silencing and differential expression under specific conditions. By contrast, we find that genes with partial methylation tend to be constitutively expressed. These patterns contrast with those found previously in other eukaryotes. By going beyond plants, animals and fungi, this stramenopile methylome adds significantly to our understanding of the evolution of DNA methylation in eukaryotes.
ISSN: 2041-1723

hal-01545322
https://hal.archives-ouvertes.fr/hal-01545322
DOI : 10.1038/ncomms3091

Éditeur(s) : HAL CCSD Elsevier
Résumé : International audience
New investigations in the Neogene Boudinar basin (Morocco) provide new information about the Messinian Salinity Crisis (MSC) and Zanclean reflooding in the southern part of the Alboran realm (westernmost Mediterranean). Based on a new field, sedimentological and palaeontological analyses, the age and the geometry of both the Messinian erosional surface (MES) and the overlying deposits have been determined. The MES is of late Messinian age and was emplaced in subaerial settings. In the Boudinar basin, a maximum of 200 m of Miocene sediments was eroded, including late Messinian gypsum blocks. The original geometry of the MES is preserved only when it is overlain by late Messinian continental deposits, conglomeratic alluvial fans or lacustrine marly sediments. These sediments are interpreted as indicators of the sea-level fall during the MSC. Elsewhere in the basin, the contact between late Messinian and early Pliocene deposits is a low-angle dipping, smooth surface that corresponds to the early Pliocene transgression surface that subsequently re-shaped the regressive MES. The early Pliocene deposits are characterized by: (i) their onlap onto either the basement of the Rif chain or the late Miocene deposits; (ii) lagoonal deposits at the base to offshore marls and sands at the top (earliest Pliocene; 5.33–5.04 Ma interval; foraminifer zone PL1); (iii) marine recovery occurring in the 5.32–5.26 Ma interval; and (iv) the change from lagoonal to offshore environments occurring within deposits tens of metres thick. This information indicates that at least the end of the reflooding period was progressive, not catastrophic as previously thought.
ISSN: 0037-0738

hal-01306672
https://hal.archives-ouvertes.fr/hal-01306672
DOI : 10.1016/j.sedgeo.2015.12.014

Éditeur(s) : HAL CCSD Seismological Society of America
Résumé : International audience
On 9 October 2007, an unusual sequence of earthquakes began in central British Columbia about 20 km west of the Nazko cone, the most recent (circa 7200 yr) volcanic center in the Anahim volcanic belt. Within 25 hr, eight earthquakes of magnitude 2.3-2.9 occurred in a region where no earthquakes had previously been recorded. During the next three weeks, more than 800 microearthquakes were located (and many more detected), most at a depth of 25-31 km and within a radius of about 5 km. After about two months, almost all activity ceased. The clear P- and S-wave arrivals indicated that these were high-frequency (volcanic-tectonic) earthquakes and the b value of 1.9 that we calculated is anomalous for crustal earthquakes but consistent with volcanic-related events. Analysis of receiver functions at a station immediately above the seismicity indicated a Moho near 30 km depth. Precise relocation of the seismicity using a double-difference method suggested a horizontal migration at the rate of about Graphic, with almost all events within the lowermost crust. Neither harmonic tremor nor long-period events were observed; however, some spasmodic bursts were recorded and determined to be colocated with the earthquake hypocenters. These observations are all very similar to a deep earthquake sequence recorded beneath Lake Tahoe, California, in 2003-2004. Based on these remarkable similarities, we interpret the Nazko sequence as an indication of an injection of magma into the lower crust beneath the Anahim volcanic belt. This magma injection fractures rock, producing high-frequency, volcanic-tectonic earthquakes and spasmodic bursts.
ISSN: 0037-1106

hal-00854504
https://hal.archives-ouvertes.fr/hal-00854504
DOI : 10.1785/0120100013

Éditeur(s) : HAL CCSD Elsevier
Résumé : International audience
We report paleomagnetic results from four overlapping stratigraphic sections (55 flows) through the lava pile in Tjörnes peninsula (North Iceland). The initial aim of our study was to check the existence of intermediate directions previously interpreted as belonging to the Matuyama-Brunhes geomagnetic reversal (MBR), and complete this record with Thellier-Thellier paleointensity determinations and 40Ar/39Ar radioisotopic dating. The directional results corroborate the findings by Kristjánsson et al. (1988): a sequence of reversed, transitional, normal, and transitional polarity (R-T-N-T) is found in each section. The polarity change is characterized by a jump from reversed virtual geomagnetic poles (VGPs) to a quasi-cluster of transitional VGPs located over China, followed by a second jump to a cluster of normal polarity before moving again towards intermediate polarities. Reversely magnetized flows exhibit a magnetic mineralogy well suited for paleointensity determination. Of these 25 flows, 20 yielded paleointensity estimates of good quality. Unfortunately, the systematic presence of coarse multidomain (MD) titanomagnetite in the transitional and normal polarity flows hampered paleointensity estimates for these flows. The Virtual Dipole Moments (VDM) calculated for the reversed polarity flows vary from 4.9 to 7.0 × 1022 Am2 with an arithmetic mean value of 5.5 ± 0.8 × 1022 Am2. This value is identical to the mean VDM obtained for the 0.3-5 Ma time window and thus strengthens the conclusion that the recent geomagnetic field strength is anomalously high compared to that older than 0.3 Ma. 40Ar/39Ar isochron ages were obtained with the incremental heating technique on groundmass separates or phenocryst-poor whole rock samples. Six of the transitionally magnetized lavas yielded isochron ages that are indistinguishable from one another at the 95% confidence level. The weighted average age is 862 ± 51 ka. In the upper part of this volcanic sequence, the normally magnetized flows yielded a weighted average age of 787 ± 40 ka. This finding supports the conclusion that a geomagnetic excursion within the Matuyama chron is recorded in the lower part of the Tjörnes volcanic sequence, whereas only the latest portion of the MBR is recorded by flows in the upper part of the lava pile. This conclusion is supported via comparison of the VGPs obtained from the lavas with those predicted by a geomagnetic field model.
ISSN: 0012-821X

hal-00618515
https://hal.archives-ouvertes.fr/hal-00618515
https://hal.archives-ouvertes.fr/hal-00618515/document
https://hal.archives-ouvertes.fr/hal-00618515/file/Camps-epsl2011.pdf
DOI : 10.1016/j.epsl.2011.07.026

Éditeur(s) : HAL CCSD
Résumé : International audience
Sedimentary basins, through the sedimentary successions and the nature of the deposits, reflect the geology of thearea from which the sediments were derived and thus provide valuable record of hinterland tectonism. As the collisionbetween the South China and the Indochina blocks (i.e. the Indosinian orogeny) is still the object of a numberof controversies regarding, for instance, its timing and the polarity of the subduction, the sedimentary basins associatedwith this mountain belt are likely to provide clues to reconstruct its geodynamic evolution. However, boththe Sam Nua Basin (located to the south of the inner zones of the Indosinian orogeny and the Song Ma ophiolites)and the Song Da Basin (located to the north of the inner zones), northern Vietnam, are still lacking importantinformation regarding the depositional environments and the ages of the main formations that they contain.Using sedimentological and dating analyses (foraminifers biostratigraphy and U-Pb dating on detrital zircon), weprovide a new stratigraphic framework for these basins and propose a geodynamic evolution of the present-daynorthern Vietnam.During the Early Triassic, the Sam Nua Basin was mainly supplied by volcaniclastic sediments originating froman active volcanic activity. Geochemical investigations, combined with sedimentological and structural analyses,support an arc-related setting for this magmatism. This magmatic arc resulted from the subduction of a south dippingoceanic slab that once separated the South China from the Indochina blocks. During the Middle to the LateTriassic, the Sam Nua Basin underwent erosion that lead to the formation of a major unconformity, termed theIndosinian unconformity. This unconformity is interpreted to result from the erosion of the Indosinian mountainbelt, built after the continental collision between the South China and the Indochina blocks. Later, during the LateTriassic, the Sam Nua Basin experienced the deposition of very coarse material, emplaced under continental settingand representing the product of the erosion of the Indosinian mountain belt.To the North, the Song Da Basin is characterized by strongly diachronous deposits over a basal unconformitydeveloped at the expense of volcanic and volcaniclastic deposits related to the Emeishan Large Igneous Province.The sedimentary succession indicates a foreland setting during the Early to the Middle Triassic, which contrastswith the commonly assumed rift setting for these sediments. Thus, the Song Da Basin documents the formation ofthe Indosinian thrust belt, located immediately to the South of the basin.
European Geosciences Union General Assembly 2017
Vienne, Austria

insu-01510331
https://hal-insu.archives-ouvertes.fr/insu-01510331

Éditeur(s) : HAL CCSD SAGE Publications (UK and US)
Résumé : International audience
Genetic approaches have shown that several genes could modify caries susceptibility; AmelogeninX (AMELX) has been repeatedly designated. Here, we hypothesized that AMELX mutations resulting in discrete changes of enamel microstructure may be found in children with a severe caries phenotype. In parallel, possible AMELX mutations that could explain resistance to caries may be found in caries-free patients. In this study, coding exons of AMELX and exon-intron boundaries were sequenced in 399 individuals with extensive caries (250) or caries-free (149) individuals from nine French hospital groups. No mutation responsible for a direct change of amelogenin function was identified. Seven single-nucleotide polymorphisms (SNPs) were found, 3 presenting a high allele frequency, and 1 being detected for the first time. Three SNPs were located in coding regions, 2 of them being non-synonymous. Both evolutionary and statistical analyses showed that none of these SNPs was associated with caries susceptibility, suggesting that AMELX is not a gene candidate in our studied population.
ISSN: 0022-0345

hal-01545481
https://hal.archives-ouvertes.fr/hal-01545481
DOI : 10.1177/0022034513482941