Éditeur(s) : HAL CCSD
Résumé : International audience
This paper presents the background for the calculation of anisotropic piezoelectric properties of single crystals and the graphical display of the results in two or three dimensions, and the calculation of the aggregate properties from constituent crystals and the texture of the aggregate in a coherent manner. The texture data can be obtained from a wide range of sources, including pole figure diffraction and single orientation measurements (electron backscattered diffraction, electron channelling pattern, Laue Pattern, optical microscope universal-stage). We consider the elastic wave propagation in piezoelectric crystals as an example of the interaction of electrical (2nd rank tensor), piezoelectric (3rd rank tensor) and elastic properties (4th rank tensor). In particular, we give explicit formulae for the calculation of the Voigt averaged tensor from individual orientations or from an orientation distribution function. For the latter we consider numerical integration and an approach based on the expansion into spherical harmonics. We illustrate the methods using single crystals, polycrystalline quartz measured using electron channelling patterns and ideal Curie limiting groups applied to quartz aggregates. This paper also serves as a reference paper for the mathematical tensor capabilities of the texture analysis software MTEX
Geological Society Special Publication, 409

hal-01277789
https://hal.archives-ouvertes.fr/hal-01277789
DOI : 10.1144/SP409.2

Éditeur(s) : HAL CCSD Geological Society, London
Résumé : This collection of papers presents recent advances in the study of deformation mechanisms and rheology and their applications to tectonics. Many of the contributions exploit new petrofabric techniques, particularly electron backscatter diffraction, to help understand the evolution of rock microstructure and mechanical properties.
Deformation Mechanisms, Rheology and Tectonics: Microstructures, Mechanics and Anistropy;Geological

hal-00685575
https://hal.archives-ouvertes.fr/hal-00685575

Éditeur(s) : HAL CCSD Public Library of Science
Résumé : International audience
Little information exists on the effects of ocean acidification (OA) on the digestive and post-digestive processes in marine fish. Here, we investigated OA impacts (Δ pH = 0.5) on the trophic transfer of select trace elements in the clownfish Amphiprion ocellaris using radiotracer techniques. Assimilation efficiencies of three essential elements (Co, Mn and Zn) as well as their other short-term and long-term kinetic parameters in juvenile clownfish were not affected by this experimental pH change. In complement, their stomach pH during digestion were not affected by the variation in seawater pH. Such observations suggest that OA impacts do not affect element assimilation in these fish. This apparent pCO2 tolerance may imply that clownfish have the ability to self-regulate pH shifts in their digestive tract, or that they can metabolically accommodate such shifts. Such results are important to accurately assess future OA impacts on diverse marine biota, as such impacts are highly species specific, complex, and may be modulated by species-specific metabolic processes.
ISSN: 1932-6203

hal-01564009
https://hal-univ-perp.archives-ouvertes.fr/hal-01564009
https://hal-univ-perp.archives-ouvertes.fr/hal-01564009/document
https://hal-univ-perp.archives-ouvertes.fr/hal-01564009/file/trophic%20transfer.pdf
DOI : 10.1371/journal.pone.0174344

Éditeur(s) : HAL CCSD Elsevier
Résumé : International audience
Carbonised template silica membranes were synthesised and investigated for water desalination for brackish (0.3wt%), sea water (3.5wt%) and brine (7.5wt%) NaCl solutions at temperatures range from 22 to 60°C. The membranes were prepared by a two-step base catalysed sol-gel method using tetraethyl orthosilicate TEOS as the silica precursor and triblock copolymer of Pluronic® P123 as the templating agent. The P123 silica membranes calcined under vacuum only produced high quality membranes by the interlayer-free method. The membranes were stable during testing of up to 250h under different thermal and salt concentration solutions, always delivering very high salt rejections >99.5%. It was found that by increasing the template concentration in the silica matrix from 5 to 50wt%, salt concentration polarisation was greatly reduced. As such, high water fluxes were measured up to 8.5kgm-2h-1 for brackish water and 6.6kgm-2h-1 for brine water. © 2014 Elsevier B.V.
ISSN: 0376-7388

hal-01683210
https://hal.univ-antilles.fr/hal-01683210
DOI : 10.1016/j.memsci.2014.10.026

Éditeur(s) : HAL CCSD Elsevier
Résumé : International audience
Given a graph, its hyperbolicity is a measure of how close its distance distribution is to the one of a tree. This parameter has gained recent attention in the analysis of some graph algorithms and the classification of complex networks. We study on practical improvements for the computation of hyperbolicity in large graphs. Precisely, we investigate on relations between the hyperbolicity of a graph G and the hyperbolicity of its atoms, that are the subgraphs output by the clique-decomposition invented by Tarjan [51, 65]. We prove that the maximum hyperbolicity taken over the atoms is at most one unit off from the hyperbol-icity of G and the bound is sharp. We also give an algorithm to slightly modify the atoms, called the " substitution method " , which is at no extra cost than computing the clique-decomposition, and so that the maximum hyperbolicity taken over the resulting graphs is exactly the hyperbolicity of the input graph G. An experimental evaluation of our method for computing the hyperbolicity of a given graph from its atoms is provided for collaboration networks and biological networks. Finally, on a more theoretical side, we deduce from our results the first linear-time algorithm for computing the hyperbolicity of an outerplanar graph.
ISSN: 0304-3975

hal-01540756
https://hal.inria.fr/hal-01540756
https://hal.inria.fr/hal-01540756/document
https://hal.inria.fr/hal-01540756/file/CCDL-2017.pdf
DOI : 10.1016/j.tcs.2017.06.001

Éditeur(s) : Wiley-blackwell
Résumé : Managing fisheries resources to maintain healthy ecosystems is one of the main goals of the ecosystem approach to fisheries (EAF). While a number of international treaties call for the implementation of EAF, there are still gaps in the underlying methodology. One aspect that has received substantial scientific attention recently is fisheries-induced evolution (FIE). Increasing evidence indicates that intensive fishing has the potential to exert strong directional selection on life-history traits, behaviour, physiology, and morphology of exploited fish. Of particular concern is that reversing evolutionary responses to fishing can be much more difficult than reversing demographic or phenotypically plastic responses. Furthermore, like climate change, multiple agents cause FIE, with effects accumulating over time. Consequently, FIE may alter the utility derived from fish stocks, which in turn can modify the monetary value living aquatic resources provide to society. Quantifying and predicting the evolutionary effects of fishing is therefore important for both ecological and economic reasons. An important reason this is not happening is the lack of an appropriate assessment framework. We therefore describe the evolutionary impact assessment (EvoIA) as a structured approach for assessing the evolutionary consequences of fishing and evaluating the predicted evolutionary outcomes of alternative management options. EvoIA can contribute to EAF by clarifying how evolution may alter stock properties and ecological relations, support the precautionary approach to fisheries management by addressing a previously overlooked source of uncertainty and risk, and thus contribute to sustainable fisheries.
Fish And Fisheries (1467-2960) (Wiley-blackwell), 2014-03 , Vol. 15 , N. 1 , P. 65-96

Droits : 2012 John Wiley & Sons Ltd
http://archimer.ifremer.fr/doc/00124/23522/21409.pdf
DOI:10.1111/faf.12007
http://archimer.ifremer.fr/doc/00124/23522/

Éditeur(s) : HAL CCSD Wiley
Résumé : International audience
More than half the pregnant women in the Western world report taking mild analgesics. These pharmaceutical compounds have been associated with congenital cryptorchidism in humans, the best-known risk factor for low semen quality and testicular germ cell cancer. Furthermore, some of these mild analgesics exert potent anti-androgenic effects in the male rat and several endocrine-disrupting compounds, known to alter masculinization, have also been shown to be potent inhibitors of prostaglandin (PG) synthesis similar to mild analgesics. Using a 3-day ex vivo organotypic model system based on gestational day 14.5 rat testes, we herein show that testosterone production was inhibited by paracetamol, at doses of 0.1 μm to 100 μm. Similar results were obtained for aspirin (1-100 μm) and indomethacin (10 μm). The production of the other Leydig cell hormone, Insl3, was not disrupted by exposure to paracetamol. Investigations of the gross anatomy of the testis as well as Leydig cells number and rate of gonocyte apoptosis after the 3 days of ex vivo differentiation showed no significant effect of the analgesics tested compared with controls. These data indicate therefore that mild analgesics specifically inhibit testosterone production in rat foetal testes in vitro and that these compounds had no effect on gonocyte survival. Parallel determinations of prostaglandin D2 (PGD2) production indicated that the effects of paracetamol and aspirin on PGD2 and testosterone were not connected, whereas the effects of indomethacin were correlated. We conclude that mild analgesics exert direct and specific anti-androgenic effects in rat foetal testis in our experimental setup and that the mechanism of action is probably uncoupled from the inhibition of PG synthesis.
ISSN: 0105-6263

hal-00876956
https://hal.archives-ouvertes.fr/hal-00876956
DOI : 10.1111/j.1365-2605.2012.01282.x
PUBMED : 22612476

Éditeur(s) : HAL CCSD Elsevier
Résumé : International audience
The Demerara plateau, located offshore French Guiana and Suriname, is part of a passive transform continental margin particularly prone to develop slope instabilities, probably in relation to the presence of a free distal border along its steep continental slope. Slope failure occurred at different periods (Cretaceous to Neogene) and shows an overall retrogressive evolution through time. Upslope these failure headscarp, an enigmatic regional Mio-Pliocene unconformity has been discovered through the interpretation of new academic and industrial datasets. The aim of this work is to describe and understand the origin of this surface. Our analysis shows that this unconformity is made of a series of valleys that cross-cut sedimentary strata. Each one of these valleys has a short lateral extent and is closed along two perpendicular directions, which suggests that it could correspond to a highly meandering system, or to some sub-circular depressions. The infill of these features is equivalent to the regional stratigraphic strata found outside the structures, but in a subdued position. This seems to imply that the structures have originated by a local loss of sediments at their base or by sliding processes. Furthermore, these depressions intersect each other through time, while migrating progressively downslope.We discuss a series of hypotheses that try to explain the onset and evolution of these depressions forming the Mio-Pliocene unconformity (Canyons? Slope failures? Contourite moats? Hydrate pockmarks?). Having established that these structures are depressions formed by collapse, and have many similarities with structures recently described in the literature as pockmarks associated with gas hydrate dissolution, we favor this hypothesis.We propose that these hydrate pockmarks form with a mass failure that was triggered by fluid-overpressure development at the base of the hydrate stability zone.
ISSN: 0025-3227

hal-01365920
https://hal.archives-ouvertes.fr/hal-01365920
DOI : 10.1016/j.margeo.2015.04.001

Éditeur(s) : HAL CCSD Elsevier
Résumé : International audience
Low-level exposure to polychlorinated biphenyl-153 (PCB-153) and dichlorodiphenyldichloroethylene (p-p'-DDE) can impair fetal growth; however, the exposure-response relationship and effect modifiers of such association are not well established. This study is an extension of an earlier European meta-analysis. Our aim was to explore exposure-response relationship between PCB-153 and p-p'-DDE and birth outcomes; to evaluate whether any no exposure-effect level and susceptible subgroups exist; and to assess the role of maternal gestational weight gain (GWG). We used a pooled dataset of 9377 mother-child pairs enrolled in 14 study populations from 11 European birth cohorts. General additive models were used to evaluate the shape of the relationships between organochlorine compounds and birth outcomes. We observed an inverse linear exposure-response relationship between prenatal exposure to PCB-153 and birth weight [decline of 194g (95% CI -314, -74) per 1μg/L increase in PCB-153]. We showed effects on birth weight over the entire exposure range, including at low levels. This reduction seems to be stronger among children of mothers who were non-Caucasian or had smoked during pregnancy. The most susceptible subgroup was girls whose mothers smoked during pregnancy. After adjusting for absolute GWG or estimated fat mass, a reduction in birth weight was still observed. This study suggests that the association between low-level exposure to PCB-153 and birth weight exists and follows an inverse linear exposure-response relationship with effects even at low levels, and that maternal smoking and ethnicity modify this association.
ISSN: 0160-4120

hal-01118572
https://hal-univ-rennes1.archives-ouvertes.fr/hal-01118572
PUBMED : 25314142
DOI : 10.1016/j.envint.2014.09.013

Éditeur(s) : HAL CCSD Laboratoire Arago
Résumé : International audience
As it is unlikely that successful settlement is solely a matter of chance (i.e. tofind a suitable habitat), one of the greatest challenges facing the fish larvae is how to locate therelatively rare patches of coral reef habitat on which they settle and ultimately reside as adults.The answer must lie partly in the sensory modalities of fishes at settlement. Habitat selection isonly possible if fish larvae could detect some environmental cues to select a suitable reef habitatat settlement. The present review aims at providing the latest works dealing with informationperception in coral reef fish larvae at settlement. Two decades ago, it was generally assumedthat larval behaviors and sensory abilities at settlement were considered too feeble to significantlyaffect dispersal outcomes. Several recent studies showed that recognition of suitable reefhabitats by fish larvae at settlement is based on a combination of visual, chemical and acousticcues. The first part of our review shows the main advances in the knowledge of visual, chemicaland acoustic cues used by fish larvae to detect an island, a reef, a micro-habitat, a conspecific orsome predators. The second part of our review deals with the effect of imprinting and/or innatecapabilities. The third part focuses on the different cues used at different scales and underlinessome contradictory results about the distance of transmission and detection of chemical andacoustic cues in coral reefs. Finally, as global and regional environmental changes have stressedcoral reefs to such an extent that they are either destroyed or in decline, the fourth part presentsthe effects of both anthropogenic and environmental stressors on information perception andresponse capacities in coral reef fish larvae. If polluted seawater disrupts the larval abilities tofind a suitable reef habitat, fish larvae may spend more time in the planktonic environment,resulting in increased energetic costs and predation risk, and consequently a lower larval settlement.We hypothesise that as the stability of fish communities is dependent, in part, on the stabilityof social interactions, the disruption of “larvae-habitat relationships” can have major consequencesfor larval settlement into adult population with further repercussions for the ecosystemas a whole. Overall, larval settlement of coral reef fish is an excellent example of the complexityof interactions between an organism and its environment as without perceiving environmentalcues, fish larva would have very little chance of selecting a suitable reef habitat. Moreover,understanding the relationship between reef state and settlement potential will allow managementplanning for the maintenance of coral reefs that are increasingly degraded.
ISSN: 0240-8759

hal-01191776
https://hal-univ-perp.archives-ouvertes.fr/hal-01191776

Éditeur(s) : HAL CCSD Elsevier
Résumé : International audience
Since the Shea, J.B. and Morgan (1979) study, investigators have repeatedly shown that the learning of a set of movement patterns (as evaluated in a retention test) is enhanced when acquisition occurred under a random rather than a blocked schedule of practice. Supposedly, this is the case because a random schedule of practice necessitates more elaborate cognitive activities than a blocked schedule before each acquisition trial can be initiated. Our main objective was to determine whether the advantage for learning found for random practice increases as a function of the number of acquisition trials. During acquisition, the results indicated a general tendency for smaller movement reproduction errors under a blocked rather than a random schedule of practice. However, this effect disappeared with larger amount of acquisition trials. In retention, larger errors were observed when acquisition occurred under a blocked rather than a random schedule of practice. Finally, the disadvantage for learning observed for the blocked schedule of practice disappeared under a block-repeated condition in which each movement pattern was first practiced under a blocked schedule which was then repeated a second time. The results are discussed in terms of the so-called contextual interference effect and indicates some of its limitations.
ISSN: 0001-6918

hal-01662846
https://hal.archives-ouvertes.fr/hal-01662846
DOI : 10.1080/00222895.1994.9941657
PUBMED : 8165923

Éditeur(s) : HAL CCSD
Résumé : International audience
Atomic Energy Review

hal-01319666
http://hal.univ-reunion.fr/hal-01319666
DOI : 10.1016/j.energy.2015.07.089

Éditeur(s) : Elsevier
Résumé : A comprehensive analysis of velocity data from subsurface floats in the northwestern tropical Atlantic at two depth layers is presented: one representing the Antarctic Intermediate Water (AAIW, pressure range 600-1050dbar), the other the upper North Atlantic Deep Water (uNADW, pressure range 1200-2050dbar). New data from three independent research programs are combined with previously available data to achieve blanket coverage in space for the AAIW layer, while coverage in the uNADW remains more intermittent. Results from the AAIW` mainly confirm previous studies on the mean flow, namely the equatorial zonal and the boundary currents, but clarify details on pathways, mostly by virtue of the spatial data coverage that sets float observations apart from e.g. shipborne or mooring observations. Mean transports in each of five zonal equatorial current bands is found to be between 2.7 and 4.5 Sv. Pathways carrying AAIW northward beyond the North Brazil Undercurrent are clearly visible in the mean velocity field, in particular a northward transport of 3.7Sv across 16 degrees N between the Antilles islands and the Mid-Atlantic Ridge. New maps of Lagrangian eddy kinetic energy and integral time scales are presented to quantify mesoscale activity. For the uNADW, mean flow and mesoscale properties are discussed as data availability allows. Trajectories in the uNADW east of the Lesser Antilles reveal interactions between the Deep Western Boundary Current (DWBC) and the basin interior, which can explain recent hydrographic observations of changes in composition of DWBC water along its southward flow. (C) 2009 Elsevier Ltd. All rights reserved.
Deep Sea Research Part I: Oceanographic Research Papers (0967-0637) (Elsevier), 2009-10 , Vol. 56 , N. 10 , P. 1615-1632

Droits : 2009 Elsevier Ltd All rights reserved.
http://archimer.ifremer.fr/doc/2009/publication-6815.pdf
DOI:10.1016/j.dsr.2009.06.002
http://archimer.ifremer.fr/doc/00000/6815/

Résumé : Tahiti’s pearl farming industry plays a major socio-economic role in French Polynesia. In an increasingly competitive market where the production of high quality pearls becomes essential, research can help secure and ensure sustainable production. In that context, Ifremer, in close collaboration with the “direction des resources marines” (French Polynesian government agency) has developed research projects on the “sustainable development of pearl farming”. This program is organized along 3 axes: (1) understanding the animal physiology and initiating a genetically selective breeding program of donor oysters; (2) understanding pearl oyster larvae dispersal and recruitment; (3) understanding the molecular mechanisms underlying biomineralization processes during shell and pearl formation. It is in this frame that we have developed a highthroughput Expressed Sequence Tags pyrosequencing program on the calcifying mantle, combined with proteomic analyses of the shell and pearl. We analyzed 276738 EST sequences, leading to the constitution of a P. margaritifera mantle transcripts catalogue of 82 sequences potentially involved in biomineralization. Our results provided direct evidence that our ESTs data set covers a large number of the matrix proteins of P. margaritifera. In addition, our proteomic analysis enabled us to retrieve, in silico, all the sequences from P. margaritifera involved in the biomineralization process already published on databases. Integration of these two methods allowed, for the first time, the global composition of calcifying tissue and calcified structures to be examined in tandem.
Recent Advances in Pearl Research—Proceedings of the International Symposium on Pearl Research 2011.Edited by Shugo Watabe, Kaoru Maeyama and Hiromichi Nagasawa. 2013. ISBN : 978-4-88704-158-5. pp.184-194

Droits : 2013 TERRAPUB. All rights reserved.
http://archimer.ifremer.fr/doc/00167/27840/26049.pdf
http://archimer.ifremer.fr/doc/00167/27840/

Éditeur(s) : HAL CCSD Springer Verlag (Germany)
Résumé : International audience
A hybrid linear ion-trap Fourier-transform ion cyclotron resonance mass spectrometer was used for top-down characterization of the abundant human salivary Cystatins, including S, S1, S2, SA, SN, C, and D, using collisionally activated dissociation (CAD) after chromatographic purification of the native, disulfide intact proteins from saliva. Post-translational modifications and protein sequence polymorphisms arising from single nucleotide polymorphisms (SNPs) were assigned from precursor and product ion masses at a tolerance of 10 ppm allowing confident identification of individual intact mass tags. Cystatins S, S1, S2, SA and SN were cleaved of a N-terminal 20 amino-acid signal peptide, and Cystatin C a 26-residue peptide, to yield a generally conserved N-terminus. In contrast, Cystatin D isoforms with 24 and 28 amino-acid residue N-terminal truncations were found such that their N-termini were not conserved. Cystatin S1 was phosphorylated at Ser3, while S2 was phosphorylated at Ser1 and Ser3 of the mature protein, in agreement with previous work. Both Cystatin D isoforms carried the polymorphism C46R (SNP: rs1799841). The 14328 Da isoform of Cystatin SN previously assigned with polymorphism P31L due to a SNP (rs2070856) was found only in whole saliva. Parotid secretions contained no detectable Cystatins while whole saliva largely mirrored the contents of submandibular/sublingual (SMSL) secretions. Top-down high-resolution mass spectrometry is a powerful tool for the identification and characterization of potential protein biomarkers in saliva.
ISSN: 1044-0305

hal-00677483
https://hal.archives-ouvertes.fr/hal-00677483
DOI : 10.1016/j.jasms.2010.01.025

Éditeur(s) : HAL CCSD Geological Society of London
Résumé : International audience
Since the early descriptions published by Callaway in 1884, the gently dipping mylonites exposed along the Moine Thrust at the Stack of Glencoul have drawn generations of geologists to the northern part of the Assynt district. These mylonites, derived from Cambrian quartzites (footwall) and Moine pelites and psammites (hanging wall), have figured prominently in: a) early research into the influence of crystal plastic deformation and recrystallization on microstructural and crystal fabric evolution; b) debates on the kinematic interpretation of macro- and micro-structures and crystal fabrics; and c) debates on the tectonic significance of such kinematic data. In this paper first we briefly review the historical aspects of this research and then, using both previously published and unpublished data, document the finite strain and quartz fabric development at this classic mylonite locality. A tectonic interpretation of these data, together with quantitative estimates of flow vorticities associated with mylonite formation at the Stack of Glencoul, are presented in a companion paper by Law (2010).
Continental Tectonics and Mountain Building: The Legacy of Peach and Horne, Geological Society Speci

hal-00564962
https://hal.archives-ouvertes.fr/hal-00564962

Éditeur(s) : HAL CCSD Elsevier
Résumé : International audience
Mesoporous cobalt oxide silica matrices and membranes were successfully prepared using a dual acid-base catalysed sol-gel and interlayer-free approach for desalination applications. The effects of cobalt addition (0-35 mol%) on the structure-property relationship of the silica materials and membrane performance were systematically studied as a function of feed salt concentration (0.3-7.5 wt.% NaCl) and temperature (22-60 °C). As cobalt concentration increases beyond 5 mol%, the concentration of tricobalt tetroxide and proportion of silica mesopores were found to increase within the matrices. Silica membranes with high amount of tricobalt tetroxide (35 mol%) were found to be highly mesoporous with water fluxes of 20, 11.3 and 7.7 kg m-2 h-1 for the 0.3, 3.5 and 7.5 wt.% solutions at 60 °C, respectively, with >99.7% salt rejection at all times. This is attributed to the high pore volume (mesopores formation) and structural robustness of the silica network provided by the presence of tricobalt tetroxide. Even higher performance was achieved for low tricobalt tetroxide (mol%) silica membranes with water fluxes of 47.6 (0.3 wt.%) and 31.5 (3.5 wt.%) kg m-2 h-1 at 60 °C, respectively, though with reduced salt rejections of 90%. © 2015 Elsevier B.V.
ISSN: 0011-9164

hal-01683209
https://hal.univ-antilles.fr/hal-01683209
DOI : 10.1016/j.desal.2015.02.034

Éditeur(s) : HAL CCSD Oxford University Press
Résumé : International audience
The BioMart Community Portal (www.biomart.org) is a community-driven effort to provide a unified interface to biomedical databases that are distributed worldwide. The portal provides access to numerous database projects supported by 30 scientific organizations. It includes over 800 different biological datasets spanning genomics, proteomics, model organisms, cancer data, ontology information and more. All resources available through the portal are independently administered and funded by their host organizations. The BioMart data federation technology provides a unified interface to all the available data. The latest version of the portal comes with many new databases that have been created by our ever-growing community. It also comes with better support and extensibility for data analysis and visualization tools. A new addition to our toolbox, the enrichment analysis tool is now accessible through graphical and web service interface. The BioMart community portal averages over one million requests per day. Building on this level of service and the wealth of information that has become available, the BioMart Community Portal has introduced a new, more scalable and cheaper alternative to the large data stores maintained by specialized organizations.
ISSN: 0305-1048

hal-01146849
https://hal-univ-rennes1.archives-ouvertes.fr/hal-01146849
DOI : 10.1093/nar/gkv350
PUBMED : 25897122

Éditeur(s) : HAL CCSD Elsevier
Résumé : International audience
Until recently, our ability to implement and assess spatial marine management approaches has been limited by a lack of information regarding processes that bind marine ecosystems, including habitat locations, larval and adult movement, trophic interactions, and fisher behavior. However, recent advances in habitat-mapping technologies, genetics, marine microchemistry, animal tracking and numerical modeling have greatly enhanced our knowledge of these processes. Although these advances have yet to be fully integrated into management decisions, they have the potential to revolutionize spatial marine management. Nevertheless, this revolution will require advances in our ability to share and integrate data into models of marine ecosystems.
ISSN: 1877-3435

hal-00609464
https://hal.archives-ouvertes.fr/hal-00609464
DOI : 10.1016/j.cosust.2010.02.002

Éditeur(s) : HAL CCSD Wiley-Blackwell
Résumé : International audience
Although protists are critical components of marine ecosystems, they are still poorly characterized. Here we analysed the taxonomic diversity of planktonic and benthic protist communities collected in six distant European coastal sites. Environmental deoxyribonucleic acid (DNA) and ribonucleic acid (RNA) from three size fractions (pico-, nano- and micro/mesoplankton), as well as from dissolved DNA and surface sediments were used as templates for tag pyrosequencing of the V4 region of the 18S ribosomal DNA. Beta-diversity analyses split the protist community structure into three main clusters: picoplankton-nanoplankton-dissolved DNA, micro/mesoplankton and sediments. Within each cluster, protist communities from the same site and time clustered together, while communities from the same site but different seasons were unrelated. Both DNA and RNA-based surveys provided similar relative abundances for most class-level taxonomic groups. Yet, particular groups were overrepresented in one of the two templates, such as marine alveolates (MALV)-I and MALV-II that were much more abundant in DNA surveys. Overall, the groups displaying the highest relative contribution were Dinophyceae, Diatomea, Ciliophora and Acantharia. Also, well represented were Mamiellophyceae, Cryptomonadales, marine alveolates and marine stramenopiles in the picoplankton, and Monadofilosa and basal Fungi in sediments. Our extensive and systematic sequencing of geographically separated sites provides the most comprehensive molecular description of coastal marine protist diversity to date.
ISSN: 1462-2912

hal-01191731
http://hal.upmc.fr/hal-01191731
http://hal.upmc.fr/hal-01191731/document
http://hal.upmc.fr/hal-01191731/file/Massana_2015_Marine_protist.pdf
DOI : 10.1111/1462-2920.12955