Éditeur(s) : HAL CCSD Wiley
Résumé : International audience
Sickle cell anemia (SS) is characterized by a reduced cerebral microvascular oxygen saturation (cerebral TOI), which is not associated with hemoglobin concentration. Cerebral TOI has never been studied in sickle cell-hemoglobin C disease (SC). We focused on the relationships between hemorheological alterations and cerebral TOI in sickle cell patients with no cerebral vasculopathy and on the usefulness of TOI variability to assess the cerebral vasomotion activity. The blood rheological profile, the level of cerebral TOI (spatial resolved spectroscopy) and the cerebral TOI variability, which reflects vasomotion activity, were compared between 20 healthy subjects (AA), 21 SC patients, and 21 SS patients. Cerebral TOI exhibited the following order: AA > SC > SS. The low cerebral TOI in SS patients was related to red blood cell aggregation and deformability properties. The cerebral TOI variability of SS and SC patients was increased above healthy values and vasomotion activity was negatively associated with the reduced cerebral TOI in SS patients. We demonstrated that (1) blood rheology could be involved in the reduced cerebral TOI in SS patients but not in SC patients; (2) vasomotion activity is increased in SS and SC patients to compensate for the reduced cerebral TOI.
ISSN: 0361-8609

inserm-00838966
http://www.hal.inserm.fr/inserm-00838966
http://www.hal.inserm.fr/inserm-00838966/document
http://www.hal.inserm.fr/inserm-00838966/file/2013_Waltz_American_Journalsur Hematology.pdf
DOI : 10.1002/ajh.23318

Éditeur(s) : HAL CCSD
Résumé : International audience
Metamorphic soles correspond to m to 500 m thick highly strained metamorphic rock units found beneath myloniticbanded peridotites at the base of large-scale ophiolites, as exemplified in Oman. Metamorphic soles aremainly composed of metabasalts deriving from the downgoing oceanic lithosphere and metamorphosed up togranulite-facies conditions by heat transfer from the mantle wedge. Pressure–temperature peak conditions areusually estimated at 1.00.2 GPa and 800100ºC. The absence of HP–LT metamorphism overprint implies thatmetamorphic soles have been formed and exhumed during subduction infancy. In this view, metamorphic soleswere strongly deformed during their accretion to the mantle wedge (corresponding, now, to the base of the ophiolite).Therefore, metamorphic soles and banded peridotites are direct witnesses of the dynamics of early subductionzones, in terms of thermal structure, fluid migration and rheology evolution across the nascent slab interface.Based on fieldwork and EBSD analyses, we present a detailed (micro–) structural study performed on samplescoming from the Sumeini window, the better-preserved cross-section of the metamorphic sole of Oman. Largedifferences are found in the deformation (CPO, grain size, aspect ratio) of clinopyroxene, amphibole and plagioclase,related to mineralogical changes linked with the distance to the peridotite contact (e.g., hardening due to theappearance of garnet and clinopyroxene).To model the incipient slab interface in laboratory, we carried out 5 hydrostatic annealing and simple-shear experimentson Griggs solid-medium apparatus. Deformation experiments were conducted at axial strain rates of 10-6s-1. Fine-grained amphibolite was synthetized by adding 1 wt.% water to a (Mid-Ocean Ridge) basalt powder asa proxy for the metamorphic sole (amphibole + plagioclase + clinopyroxene garnet assemblage). To synthetizegarnet, 2 experiments were carried out in hydrostatic conditions and with deformation at 800C with confiningpressure of 2 GPa. Another simple-shear experiment has been carried out at 800C and 1 GPa with fined-grainednatural garnet. With the aim of mimicking the early slab interface (between the metamorphic sole and bandedperidotites at the base of the ophiolite), 2 simple-shear deformation experiments with 2 layers have been carriedout at 800C and confining pressure of 1 GPa. The bottom layer was made of hydrated basalt powder and the toplayer was made of olivine.Fined-grained garnet-free amphibolite is significantly weaker than dunite but the appearance of harder mineralsin the amphibolite (garnet and clinopyroxene) has major implications on its rheological evolution. These resultsallow liking field observations of strain localization at the interface to the metamorphic sole formation
European Geosciences Union General Assembly 2016
Vienne, Austria

insu-01309394
https://hal-insu.archives-ouvertes.fr/insu-01309394

Éditeur(s) : HAL CCSD
Résumé : Red blood cell hemodynamics influence blood rheology and thus circulatory function. Different authors showed that red blood cells are heterogeneous in blood flow. This heterogeneity could be explained by physical characteristics such as morphology, aggregability and deformability, but also by cellular and molecular environment. All these notions are found in sickle cell disease where sickle red blood cells become more rigid, leading to vascular occlusions, increased with an inflammatory context. In a preceding study, our results showed that velocity heterogeneity allow RBC classification, leading to different RBC sub-groups, using a dynamical approach and flow studies. In order to have a better understanding of the physiological meaning of such an heterogeneity, dynamical velocity experiences have been conducted in the case of normal and sickle red blood cells with different endothelial activations by proinflammatory cytokines, under a constant venular flow. Thus, normal and sickle red blood cells are classified into different sub-groups, showing their heterogeneity. Moreover, sub-population velocities and distribution evolve differently according to vascular state or treatment. These results show that red blood cell velocities and rheology depend on vascular environment and red blood cell morphology. However, the physiological meaning of this behavior should be explained by cellular and molecular effects of such activation.
https://hal.archives-ouvertes.fr/hal-00201461

hal-00201461
https://hal.archives-ouvertes.fr/hal-00201461
https://hal.archives-ouvertes.fr/hal-00201461/document
https://hal.archives-ouvertes.fr/hal-00201461/file/RBCV_Marianne_v1.pdf

Éditeur(s) : HAL CCSD American Geophysical Union (AGU)
Résumé : International audience
The nucleation, propagation, and associated folding of ramp-flat-ramp normal faults were analyzed from field examples developed in a brittle/ductile multilayer sequence of the Huércal-Overa Basin (SE Spain). Gently dipping sandy silt layers, which display a low cohesive strength (C0 = 7 kPa, μ = 34°), favor the development of extensional detachments. A tectonic origin instead of a possible gravitational origin is supported by the perpendicularity between the paleoslope direction of the fluvial-deltaic environment inferred from imbricated pebbles, and the senses of movement deduced from fault slicken-lines. The link between high-angle normal faults (HANFs) --formed at different levels in the layered sequence-- with horizontal fault segments comes to develop ramp-flat-ramp normal faults with associated roll-over in the hanging wall. Observed extensional duplexes are formed by parallel detachments connected through synthetic Riedel faults. These Riedel faults would produce the back-rotation of the individual blocks (horses), i.e., extensional folding of the originally subhorizontal layers. There is no correlation between the analyzed ramp-flat-ramp normal faults, accommodating south-southeastward extension during Serravallian-lower Tortonian, and either the regional Alpujarride/Nevado-Filabride west-directed extensional shear zone or the top-to-the-north detachments within Alpujarride units, which are clearly sealed by Serravallian-lower Tortonian sediments. Therefore, the studied normal faults are restricted to the brittle/ductile multilayer fluvio/deltaic sequence and accommodate moderate late extension instead of belonging to a large crustal extensional system connected with a regional detachment at depth. Therefore, the basin formed in a moderate crustal thickness context where small and medium-scale extensional systems were subordinate structures. These natural examples support the development of low-angle normal faults at very shallow crustal levels in multilayer sequences with suitable rheological conditions.
ISSN: 0278-7407

hal-00750352
https://hal.archives-ouvertes.fr/hal-00750352
DOI : 10.1029/2012TC003130

Éditeur(s) : HAL CCSD
Résumé : International audience
Sub-continental lithospheric mantle rocks are exhumed at the foot of magma-poor distal passive margins as aresponse to extreme stretching of the continental crust during plate separation. Remnants of the Northern Iberianpaleo-passive margin are now exposed in the North-Pyrenean Zone (NPZ) and represent field analogues to studythe processes of continental crust thinning and subcontinental mantle exhumation. The NPZ results from theinversion of basins opened between the Iberia and Europa plates during Albo-Cenomanian times. In the westernNPZ, the ‘Chaînons Béarnais’ ranges display a fold-and-thrust structure involving the Mesozoic sedimentarycover, decoupled from its continental basement and associated with peridotite bodies in tectonic contact withPalaeozoic basement lenses of small size. Continental extension developed under hot thermal conditions, asdemonstrated by the syn-metamorphic Cretaceous ductile deformation affecting both the crustal basement and theallochthonous Mesozoic cover.In this study, we present structural and geochemical data providing constraints to reconstruct the evolution ofthe northern Iberia paleo-margin. Field work confirms that the pre-rift Mesozoic cover is intimately associatedto mantle rocks and to thin tectonic lenses of crustal basement. It also shows that the pre-rift cover was detachedfrom its bedrock at the Keuper evaporites level and was welded to mantle rocks during their exhumation atthe foot of the hyper-extended margin. The crust/mantle detachment fault is a major shear zone characterizedby anastomosed shear bands defining a plurimetric phacoidal fabric at the top of the serpentinized mantle. Thedetachment is marked by a layer of metasomatic rocks, locally 20 meters thick, made of talc-chlorite-pyrite-richrocks that developped under greenschist facies conditions. Raman Spectroscopy on Carbonaceous Materials(RSCM), performed on the Mesozoic cover reveal that the entire sedimentary pile underwent temperatures rangingbetween 200C and 480C.We show that: (i) at the site of mantle rocks exhumation, the boudinaged pre-rift sediments have undergone drasticsyn-metamorphic thinning with the genesis of a S0/S1 foliation and, (ii) the Paleozoic basement has been ductilelydeformed, into thin tectonic lenses that remained welded to the exhumed mantle rocks. Therefore the overallcrustal rheology appears dominated by shallow levels having a ductile behavior. This rheology is related to thepresence of a thick pre- and syn-rift decoupled cover acting as an efficient thermal blanket. This new geologicaldata set highlights important characteristics of ductile-type hyper-extended passive margin that cannot be obtainedfrom the study of seismic lines. Finally, we stress that studying field analogues represents a major tool to betterunderstand the mechanisms of extreme crustal thinning associated with mantle exhumation and their structuralinheritance during tectonic inversion.
Geophysical Research Abstracts
Vienne, Austria

insu-01510103
https://hal-insu.archives-ouvertes.fr/insu-01510103

Éditeur(s) : HAL CCSD Public Library of Science
Résumé : International audience
The six-minute walk test is a well-established submaximal exercise reflecting the functional status and the clinical severity of sickle cell patients. The aim of the present cross-sectional study was to investigate the biological determinants of the six-minute walk test performance in children with sickle cell anemia. Hematological and hemorheological parameters, pulmonary function and the six-minute walk test performance were determined in 42 children with sickle cell anemia at steady state. The performance during the six-minute walk test was normalized for age, sex and height and expressed as percentage of the predicted six-minute walk distance. We showed that a high level of anemia, a low fetal hemoglobin expression and low red blood cell deformability were independent predictors of a low six-minute walk test performance. This study describes for the first time the impact of blood rheology in the six-minute walk test performance in children with sickle cell anemia.
ISSN: 1932-6203

inserm-00922110
http://www.hal.inserm.fr/inserm-00922110
http://www.hal.inserm.fr/inserm-00922110/document
http://www.hal.inserm.fr/inserm-00922110/file/2013_Waltz_-_Hematological_and_hemorheological_determinants_6_minutes.pdf
DOI : 10.1371/journal.pone.0077830

Éditeur(s) : HAL CCSD
Résumé : International audience
The injection of reactive mineral suspensions is a possible process to either reinforce or seal wells in geologic carbon sequestration sites. Among others, olivine slurries could be used as CO2-triggered cements, as they carbonate under the thermodynamic conditions of deep storage. However, injection of such slurries requires to control their initial fluidity and its evolution during the displacement. For this purpose, we have characterized the rheological evolution of olivine suspensions, of particle sizes 1−10 μm, sheared at 100 s−1, in the ranges of 10−40% for the volume fractions, 20−50 bars for the CO2 pressure, and 60-110°C for the temperature. The apparent viscosity, measured at 100 s−1, increases exponentially, with a characteristic time ranging from less than 1 h to more than 10 h, and decreasing with olivine concentration, CO2 pressure, and temperature. Moreover, a careful characterization of the slurry rheology carried out in the initial stages of slow evolution, shows that the slurry obeys a Bingham plastic law, with a yield stress ΣB, and a Bingham viscosity µB increasing from 1 to 20 Pa and from 4 to 110 mPa s, respectively, when the olivine volume fraction φ is increased from 10 to 30%. Such variations can be accounted by classical models (Wildemuth and Williams [1984]: Rheologica Acta, 23,627-635, Krieger [1972]: Advances in Colloid and Interface Science, 3, 111-136).
Environmental Progress & Sustainable Energy

hal-01054131
https://hal.archives-ouvertes.fr/hal-01054131
DOI : 10.1002/ep.11826

Éditeur(s) : HAL CCSD IOS Press
Résumé : International audience
Purpose: The aim of this study was to clarify whether exercising in a tropical climate induces blood rheology alterations despite ad libitum hydration. Methods: Hematological, biochemical and hemorheological changes were investigated in young healthy adults (N = 9 men, 20.7 ± 0.8 yrs) after a 10-km race in hot and humid conditions. Subjects' maximal aerobic abilities were tested using a maximal ramp exercise. Blood was sampled at rest (T R), at the end of the race (T Ex), and after 24 hours of recovery (T 24). Ad libitum hydra-tion was allowed during the race. Blood viscosity (b), red blood cell deformability (EI), aggregation (AI) and disaggregation shear rate (␥) were measured. Results: Hematocrit, hemoglobin and plasma concentration of chlorine, sodium and potassium did not change in response to exercise. No functional consequence was observed on RBC deformability since EI remained unchanged. Percentages of echinocytes, schizocytes and stomatocytes remained in the subclinical range at all times. AI, µ and ßb did not present change. Conclusion: Running exercise in tropical climate with ad libitum hydration does not alter the main rheological properties of blood.
ISSN: 1386-0291

hal-01163975
https://hal.univ-antilles.fr/hal-01163975
DOI : 10.3233/CH-2011-1388

Éditeur(s) : HAL CCSD IOS Press
Résumé : International audience
Sickle cell anemia (SCA) is an inherited red blood cells (RBC) disorder characterized by significantly decreased RBC deformability. The present study aimed to assess whether modulation of RBC Nitric Oxide Synthase (RBC-NOS) activation could affect RBC deformability in SCA.Blood of twenty-five SCA patients was treated for 1 hour at 37°C with Phosphate Buffered Saline (PBS) or PBS containing 1% of Dimethylsulfoxyde as control, L-arginine or N(5)-(1-Iminoethyl)-L-ornithine (L-NIO) to directly stimulate or inhibit RBC-NOS, insulin or wortmannin to indirectly stimulate or inhibit RBC-NOS through their effects on the PI3 Kinase/Akt pathway, and sodium nitroprusside (SNP) and 2-(4-Carboxyphenyl)-4,4,5,5-tetramethylimidazoline-1-oxyl-3-oxide (cPTIO) as NO donor and NO scavenger, respectively. RBC deformability was measured by ektacytometry at 3 Pa.RBC deformability significantly increased after insulin treatment and significantly decreased after L-NIO and wortmannin incubation. The other conditions did not affect deformability. Significantly increased nitrotyrosine levels, a marker of enhanced free radical generation, were detected by immunohistochemistry in SNP and insulin treated samples.These data suggest that RBC deformability of SCA can be modulated by RBC-NOS activity but also that oxidative stress may impair effectiveness of RBC-NOS produced NO.
ISSN: 1386-0291

hal-01669738
https://hal.univ-antilles.fr/hal-01669738
DOI : 10.3233/CH-162042

Éditeur(s) : HAL CCSD IOS Press
Résumé : The main objectives of the present work were to test the effects of heat stress on blood rheology and to determine whether the responses can change according to the pig breeds. Thirty-six pigs from three pig's lines (n = 12 for each line) with assumed different tolerance to heat stress were compared: Large White (LW, little tolerance), Creole (CR, good tolerance) and LW × CR pigs (produced from a cross between LW and CR lines). In a first period, all pigs were exposed to a 9-d period of thermo-neutral environment (24°C; d-9 to d-1; P0). At the end of P0, six pigs from each line were slaughtered (n = 18). Then in a second period, the remaining pigs (6/breed; n = 18) were exposed to a 5-d period of heat stress (32°C; d + 1 -d + 5; P1) and thereafter slaughtered at d + 5. Rectal and skin temperatures, as well as respiratory rate, were recorded on d-1 and d + 5. At slaughter, blood was sampled for hematological and hemorheological measurements. Heat stress caused a rise of the skin temperature and respiratory rate without any changes in the rectal temperature or on the hematological and hemorheological parameters when all pigs' lines were considered. We observed a pig line effect on blood viscosity at high shear rate (375 s-1) and red blood cell deformability at 30 Pa with CR pigs having lower blood viscosity and higher red blood cell deformability than LW pigs. While the changes of blood viscosity under heat stress did not reach statistical significance in LW and CR lines, blood viscosity (at 375 s-1) increased above the temperate values in the LW × CR line. Red blood cell deformability at 30 Pa was higher in CR pigs exposed to heat stress compared to LW pigs in the same condition. In conclusion, thermal loading caused physiological stress but did not widely change the hematological and hemorheological profiles. Although some blood rheological parameters seem to vary with the pig breeds, the responses to heat stress are very similar.
ISSN: 1386-0291

hal-01210926
https://hal.archives-ouvertes.fr/hal-01210926
DOI : 10.3233/CH-131722
PRODINRA : 286489
PUBMED : 23603327

Éditeur(s) : HAL CCSD Elsevier
Résumé : International audience
Regional-scale deformation taking place in a strongly anisotropic, yet homogeneous metapelitic protolith during an apparently single tectonic event was systematically investigated as a function of the distance to the main tectonic contact (i.e., the Filabres shear zone, a major detachment in the Betic Cordilleras, Spain). The density of C3' shear bands (or extensional crenulation cleavage) reworking the earlier S2 schistosity increases exponentially towards the contact, in parallel with the decrease in the size of the shear domains. Systematic variations in angles and shape ratios are also reported. Deformation and age patterns, however, suggest that this spectacular trend at least partly results from a progressive localization of the deformation through time. This fossilized shear strain gradient was thus produced somewhat diachronously. Such shear strain patterns nevertheless provide a mean to constrain the rheological properties for such weak lithologies and a mean to better understand crustal deformation.
ISSN: 0191-8141

insu-00545043
https://hal-insu.archives-ouvertes.fr/insu-00545043
https://hal-insu.archives-ouvertes.fr/insu-00545043/document
https://hal-insu.archives-ouvertes.fr/insu-00545043/file/JSGagardetal2.pdf
DOI : 10.1016/j.jsg.2010.11.011

Éditeur(s) : HAL CCSD ACSM
Résumé : International audience
A 26-yr-old man with sickle cell trait (SCT) suddenly lost visual acuity in the left eye after a cycling race in hot tropical environment. The cause was massive central retinal vein occlusion (CRVO) with hemorrhaging that rapidly worsened to neovascular glaucoma. Although medically treated, the eye is now marked by total retinal detachment. Cardiovascular function assessment shows no electrocardiographic abnormalities, no anomaly in the supra-aortic tree, and no evidence of structural heart disease. Although normal coagulation markers values (i.e., activated partial thromboplastin time, prothrombin time, fibrinogen concentration, antithrombin III, factor V, proteins C and S) were observed 2.5 months after the clinical event, a transesophageal echocardiogram performed few hours after the incident revealed the presence of four thrombi in the left atrium suggesting a postexercise hypercoagulable state at that time. Hemorheological measurements at distance of the events demonstrated high red blood cell rigidity at baseline. Therefore, marked blood rheological impairment and activation of the coagulation pathway in response to the heavy and prolonged cycling race could have promoted CRVO in this cyclist carrying SCT. These data suggest that SCT could be considered as a risk factor for significant ocular complications when severe exercise is performed and support the idea that SCT is a contributing factor in blood rheology and vascular dysfunctions.
Medicine & Science in Sports & exercise

Droits : info:eu-repo/semantics/OpenAccess
hal-00698572
https://hal.univ-antilles.fr/hal-00698572
https://hal.univ-antilles.fr/hal-00698572/document
https://hal.univ-antilles.fr/hal-00698572/file/Hedreville_et_al_revised_MSSE-D-08-00064R2.pdf
DOI : 10.1249/MSS.0b013e31818313d0

Éditeur(s) : HAL CCSD Elsevier
Résumé : International audience
Studies performed in the last decades have highlighted the need to better understand the contribution of the endothelium, vascular function, oxidative stress, inflammation, coagulation, hemolysis and vascular adhesion mechanisms to the pathophysiology of acute vaso-occlusive like events and chronic organ damages in sickle cell disease (SCD). Although SCD is a hemorheological disease, a few works focused on the contribution of blood viscosity, plasma viscosity, red blood cell deformability and aggregation in the pathophysiology of SCD. After a brief description of basic hemorheology, the present review focuses on the role of the hemorheological abnormalities in the causation of several SCD complications, mainly in sickle cell anemia and hemoglobin (Hb) SC disease. Several genetic and cellular modulators of blood rheology in SCD are discussed, as well as unresolved questions and perspectives.
ISSN: 0268-960X

hal-01668334
https://hal.univ-antilles.fr/hal-01668334
DOI : 10.1016/j.blre.2015.08.005

Éditeur(s) : HAL CCSD Elsevier
Résumé : International audience
Deformation of the continental lithosphere is traditionally modeled assuming that heat production in the subcontinental lithospheric mantle is negligible. Although this may be appropriate for highly depleted Archean lithosphere, studies of mantle xenoliths and lithospheric-derived mafic rocks suggest that heat productions of up to 0.4 ;μW/m3 may be attained in the mantle of Phanerozoic and Neoproterozoic regions due to modal metasomatism. To investigate the effect of a local enrichment in heat-producing elements within the lithospheric mantle on the continental deformation, we performed a series of 2D thermo-mechanical models simulating the deformation of a lithospheric plate 1000 km long, which contains in its central part a 200 km wide and 20 km-thick mantle layer with a heat production of 0.05;–0.25 ;μW/m3 between 40 and 80 km depth, leading to a local enhancement of the surface heat flow by 1;–5 mW/m2. Compression models show that a 20 km-thick layer with a heat production as low as 0.05 ;μW/m3 within the shallow lithospheric mantle leads to strain localization in both the enriched mantle and the overlying crust. Strain localization depends exponentially on the temperature increase in the lithosphere section, which is controlled by the volume of metasomatized mantle and the intensity of the metasomatic enrichment in heat-producing elements. A heat production of 0.25 μW/m3 between 60 and 80 km depth results, for instance, in strains within and above the metasomatized mantle 5 times higher than in the surrounding lithosphere. Strain localization also depends on the location of the heat-producing domain and on the plate rheological structure; deeper heat-producing domains and weaker plates leading to stronger localization. These results provide an explanation for strain localization in intraplate environments, leading to the formation of orogenic belts situated hundreds to thousands of kilometers away from known plate boundaries, as observed in the Neoproterozoic belts of Gondwana and in the present-day deformation of the Asian plate.
ISSN: 0012-821X

hal-00411386
https://hal.archives-ouvertes.fr/hal-00411386
DOI : 10.1016/j.epsl.2008.07.040

Résumé : Nous avons étudié les marqueurs hémorhéologiques, hématologiques et biochimiques chez des sujets drépanocytaires homozygotes SS (HbS/HbS) et hétérozygotes composites SC (HbS/HbC) dans deux cohortes, pédiatriques et adultes, de patients drépanocytaires, et ce, à travers 7 complications récurrentes de la drépanocytose : 2 appartenant au profil hémolytique (l’ulcère de jambes et la glomérulopathie) et 5 appartenant au phénotype visqueux/vaso-occlusif (l’hypertension artérielle, le syndrome thoracique aigu (STA), la crise vaso-occlusive (CVO), la rétinopathie et l’ostéonécrose). Nous avons montré que : 1) une viscosité sanguine et une déformabilité érythrocytaire élevées sont des facteurs de risques de CVO chez les enfants homozygotes ; 2) Une viscosité sanguine élevée est associée à une hypertension artérielle systémique relative chez des adultes SS ; 3) les enfants SC présente une fonction vasculaire mieux préservée que les enfants SS pour faire face à une augmentation de la viscosité sanguine ; 4) les patients adultes SS avec une ostéonécrose présentent une déformabilité érythrocytaire plus élevée que les patients sans ostéonécrose ; 5) une viscosité sanguine élevée est associée à la présence d’une rétinopathie chez les adultes SC mais pas chez les SS ; 6) les patients adultes SS présentant une glomérulopathie ont un taux d’hémolyse élevé, une déformabilité érythrocytaire réduite et des agrégats érythrocytaires très robustes ; 7) les patients adultes SS avec des ulcères de jambes récurrents ont un taux d’hémolyse accru et une déformabilité érythrocytaire réduite. De plus, nos travaux confirment que l’-thalassémie module les propriétés de déformabilité érythrocytaire, mais montrent pour la première fois qu’elle module aussi les propriétés d’agrégation érythrocytaire, et notamment la force des agrégats érythrocytaires. En conclusion, ces travaux permettent de préciser le rôle de la rhéologie sanguine dans un certain nombre de complications de la drépanocytose et d’enrichir le modèle préexistant divisant les complications de la drépanocytose selon 2 phénotypes : hémolytique versus visqueux/vaso-occlusif. Nous montrons pour la première fois que le phénotype hémolytique est caractérisé aussi par des anomalies de la rhéologie du globule rouge : rigidité accrue et agrégats érythrocytaire robustes.
Hemorheological, hemathological, and biochemical marquers of patients with sickle cell anemia (SS) and patients with sickle cell SC disease (SC) were studied in 2 cohorts: children and adults. We focused on 7 recurrent complications: 5 belonging to the viscosity/vaso-occlusion phenotype (systemic hypertension, acute chest syndrome (ACS), vaso-occlusive crisis (VOC), retinopathy and osteonecrosis) and 2 belonging to the hemolytic phenotype (leg ulcer and glomerulopathy). Our results show that 1) high viscosity is associated with increased risk for VOC in SS children; 2) blood viscosity is increased in SS adults with systemic relative hypertension; 3) SC children have preserved vascular function compared to SS children; 4) SS adults with osteonecrosis are characterized by higher red blood cell (RBC) deformability than SS adults without osteonecrosis; 5) high blood viscosity is associated with retinopathy in SC adults but not in SS adults; 6) SS adults affected by glomerulopathy have high hemolytic rate, low RBC deformability and increased RBC aggregates strenght; 7) SS adults with recurrent leg ulcers have high hemolytic rate and reduced RBC deformability. Moreover, our studies shows that alpha-thalassemia modulate RBC deformability and RBC aggregation properties. In conclusion, this work shows for the first time that the hemolytic phenotype is characterized by an abnormal RBC rheology which may play a role in several sickle cell complications.

http://www.theses.fr/2013AGUY0684/document

Éditeur(s) : HAL CCSD
Résumé : High Pressure - Low Temperature (HP-LT) rocks testify for burying and exhumation of crustal material in subduction zones. Their complete exhumation is the result of processes acting during (syn-orogenic) and after (post-orogenic) subduction is active, the latter partly obliterating the first. In the Aegean domain, the Attic-Cycladic Blueschist unit (ACBU) is a HP-LT unit exhumed in metamorphic core complexes (MCCs) in the back-arc domain of the Hellenic subduction zone. This study is an attempt to distinguish features related to syn-orogenic exhumation processes responsible for the first stages of exhumation This syn-orogenic "message" has to be read through the post-convergence structural and thermal overprint that accompanied the formation of the MCCs. Particularly, the geometry, the timing and the amount of the syn-orogenic exhumation has to be determined. We investigate these features with coupled field, metamorphic petrology and radiochronology studies First, based on observations on Ios (southern Cyclades), it has recently been proposed that syn-orogenic exhumation of the ACBU was accommodated by a basal thrust over the Cycladic Basement (CB) [Huet, et al., 2009]. The peak conditions, deduced from pseudosections calculation for the Ios main lithologies, are 500 °C-19 kbar for the ACBU and 550 °C-16 kbar for the CB. Exhumation until 400 °C-8 kbar is considered as syn-orogenic. Second, phengites populations, single grains and in situ 40Ar-39Ar geochronology has been carried out on samples from Tinos and Andros (northern Cyclades), Syros (central Cyclades) and Ios. The results gather in several clusters: 50-55 Ma, 40-45 Ma, 30-35 Ma, and 22-26 Ma. Based on these new P-T-t data and a synthesis of published data, we are able to refine the geometry and the timing of the exhumation of the ACBU. Data from the whole Cyclades exhibit little differences in ages and P-T conditions for the successive stages of exhumation. The peak of pressure (500±50 °C-18±1 kbar) has been reached at 50-55 Ma from Tinos to Ios. The onset of the exhumation occurred at 40-45 Ma in blueschist facies conditions, between a thrust at their bottom and a detachment at their top. The following decompression was accompanied by cooling until 30-35 Ma (400±50 °C-8±1 kbar). At that time, the boundary conditions switched to extension. Finally, we propose a model for the exhumation of HP-LT units accounting for these data. We also consider the rheological implications of the syn-orogenic structuration of the Aegean wedge on the post-orogenic evolution. B. Huet, L. Labrousse, and L. Jolivet [2009] Thrust or detachment? Exhumation processes in the Aegean: Insight from a field study on Ios (Cyclades, Greece), Tectonics, 28, 3, doi:10.1029/2008TC002397.
EGU General Assembly
Vienne, Austria

insu-00839054
https://hal-insu.archives-ouvertes.fr/insu-00839054
BIBCODE : 2010EGUGA..1214098H

Éditeur(s) : HAL CCSD Elsevier
Résumé : International audience
The lithospheric mantle is intrinsically heterogeneous and anisotropic. These two properties govern the repartition of deformation, controlling intraplate strain localization and development of new plate boundaries. Geophysical and geological observations provide clues on the types, ranges, and characteristic length scales of heterogeneity and anisotropy in the lithospheric mantle. Seismic tomography points to variations in geothermal gradient and hence in rheological behavior at scales of hundreds of km. Seismic anisotropy data substantiate anisotropic physical properties consistent at scales of tens to hundreds of km. Receiver functions imply lateral and vertical heterogeneity at scales < 10 km, which might record gradients in composition or anisotropy. Observations on naturally deformed peridotites establish that compositional heterogeneity and Crystal Preferred Orientations (CPOs) are ubiquitous from the mm to the km scales. These data allow discussing the processes that produce/destroy heterogeneity and anisotropy and constraining the time scales over which they are active. This analysis highlights: (i) the role of deformation and reactive percolation of melts and fluids in producing compositional and structural heterogeneity and the feedbacks between these processes, (ii) the weak mechanical effect of mineralogical variations, and (iii) the low volumes of fine-grained microstructures and difficulty to preserve them. In contrast, olivine CPO and the resulting anisotropy of mechanical and thermal properties are only modified by deformation. Based on this analysis, we propose that strain localization at the plate scale is, at first order, controlled by large-scale variations in thermal structure and in CPO-induced anisotropy. In cold parts of the lithospheric mantle , grain size reduction may contribute to strain localization, but the low volume of fine-grained domains limits this effect.
ISSN: 0040-1951

hal-01277174
https://hal.archives-ouvertes.fr/hal-01277174
DOI : 10.1016/j.tecto.2015.07.026

Éditeur(s) : HAL CCSD IOS Press
Résumé : International audience
The aim of the present study was to test the effects of hydroxyurea (HU) therapy on clinical, hematological and hemorheological parameters in adult patients with sickle cell anemia (SCA). Hematological and hemorheological parameters were measured in 28 SCA patients before HU therapy (i.e., baseline) and at 6, 12 and 24 months of treatment. RBC deformability was determined by ektacytometry at 30 Pa. RBC aggregation properties were investigated by light-backscatter method. Blood viscosity was measured at 225 s-1 by a cone-plate viscometer. The rates of vaso-occlusive crises and acute chest syndrome were lower at 1 and 2 years of HU therapy compared to baseline. The proportion of patients with leg ulcers tended to decrease after 2 years of treatment. Hemoglobin oxygen saturation improved with HU therapy. HU therapy induced a decrease of platelet and white blood cell counts and a rise in fetal hemoglobin level and mean cell volume. While hemoglobin concentrations increased under HU, blood viscosity remained unchanged all along the study. RBC deformability increased over baseline values at 6 months of HU therapy and continued to rise until the end of the follow-up period. In conclusion, the improvement in RBC deformability probably compensates the increase of hemoglobin on blood viscosity and participates to the improvement of the clinical status of patients.
ISSN: 1386-0291

hal-01668327
https://hal.univ-antilles.fr/hal-01668327
DOI : 10.3233/CH-170280

Éditeur(s) : HAL CCSD
Résumé : International audience
Mylonite-bearing crustal shear zones are exhumed extensions at depth of (potentially seismogenic) brittle faultsat shallow structural level. By concentrating deformation, shear zones play a major role in the rheology and mechanicalbehavior of the continental crust. Analysis of shear zone geometry and microstructures is a useful toolto decipher deformation kinematics, though finite strain estimation is still relatively qualitative. Timing of thedeformation has also been the focus of numerous studies using the complete spectrum of geochronometers. However,only rare examples provide geochronological data that can be unambiguously linked with deformation. Thus,time-scales over which major mylonite zones develop and remain active under ductile conditions as well as theirstrain rates often remain poorly documented. Several sections across the South Armorican Shear Zone (SASZ),a crustal-scale, several km-thick dextral shear zone were investigated by structural, petrological and Ar/Ar radiochronologicalmethods. Finite strain profiles on these sections showed a strong deformation partitioning towardsthe ultramylonite core of the SASZ, with a decrease, of both the C’/S mean angle (from ca. 45 to less than5) and the Quartz grain-size (from ca. 150 to 5 m). In parallel, conventional and in-situ Ar/Ar datings on thefabric-forming white micas were performed on compositionally complex white-micas. Inherited magmatic muscovitescarried by the foliation and newly-formed, syn-tectonic substituted phengites located along the shear bandsyielded 2 age groups for the less deformed samples. Along the shear zone cross-section, the difference betweenthese two ages evolves from 10-15 Ma in weakly deformed domains to zero toward the SASZ core. In addition, thevery fine-grained syn-tectonic phengites yielded the same ca. 300-298 Ma age irrespectively of their distance to thecore of the shear zone, even in slightly deformed rocks distant by several km. These results support a new modelof crustal-scale shear zone activity, where the whole body of the shear zone is active throughout its history, i.e.the actively deforming zone does not get progressively narrower. The strain distribution nevertheless evolves withtime, with an increasingly larger fraction partitioned in the ultramylonitic core. Besides, combination of the finitestrain estimations and the Ar/Ar ages enables us to assess the range of strain rates associated with mylonitization.
EGU General Assembly 2014
Vienne, Austria

insu-01565057
https://hal-insu.archives-ouvertes.fr/insu-01565057

Éditeur(s) : HAL CCSD Elsevier
Résumé : International audience
Orogenic wedges locally present chaotic tectonostratigraphic units that contain exotic blocks of various size, origin, age and lithology, embedded in a sedimentary matrix. The occurrence of ophiolitic blocks, sometimes huge, in such “mélanges” raises questions on (i) the mechanisms responsible for the incorporation of oceanic basement rocks into an accretionary wedge and (ii) the mechanisms allowing exhumation and redeposition of these exotic elements in “mélanges” during wedge growth.To address these questions, we present the results of a series of analogue experiments performed to characterize the processes and parameters responsible for accretion, exhumation and tectonosedimentary reworking of oceanic basement lithospheric fragments in an accretionary wedge.The experimental setup is designed to simulate the interaction between tectonics, erosion and sedimentation. Different configurations are applied to study the impact of various parameters, such as irregular oceanic floor due to structural inheritance, or the presence of layers with contrasted rheology that can affect deformation partitioning in the wedge (frontal accretion vs basal accretion) influencing its growth. Image correlation technique allows extracting instantaneous velocity field, and tracking of passive particles. By retrieving the particle paths determined from models, the pressure-temperature path of mélange units or elementary blocks can be discussed. The experimental results are then compared with observations from ophiolite-bearing mélanges in Taiwan (Lichi and Kenting mélanges) and Raman spectroscopy of carbonaceous material (RSCM) Thermometry data on rocks from the northern Apennines (Casanova mélange). A geological scenario is proposed following basic observations. The tectonic evolution of the retroside of doubly vergent accretionary wedges is mainly controlled by backthrusting and backfolding. The retro wedge is characterized by steep slopes that are prone to gravitational instabilities. It triggers submarine landslides inducing huge mass transfers. This erosion combined with backthrusting could favour exhumation of the ophiolitic fragments formerly accreted at the base of the wedge along the rough seafloor-sediments interface. Such an exhumed material can be reworked and deposited as debris- flows in proximal basins located at the foot of the retrowedge slope forming a tectono-sedimentary mélange. These syntectonic basins are continuously deformed and involved in prograding backthrusting-induced deformation.
ISSN: 0264-3707

hal-01395127
https://hal.archives-ouvertes.fr/hal-01395127
DOI : 10.1016/j.jog.2016.05.008