Éditeur(s) : HAL CCSD Elsevier
Résumé : International audience
The Longmen Shan range is one of the major topographic and structural features of the easternmargin of the Tibetan Plateau. With an impressive topographic gradient and low convergence rates across the range this region has raised important questions concerning the dynamics of plateaumargin settings, such as the long-term mechanisms of topographic evolution. The investigation of the distribution in space and time of denudation can provide critical insight into such dynamics and shed light on still unresolved controversies. For that purpose, we present a new dataset that documents the intensity and distribution of denudation processes across this plateaumargin through field survey of fluvial incision markers, quantitative geomorphology and cosmogenic nuclide derived basin-wide erosion rates. Erosion is < 0.5 mm/year in the frontal region of the Longmen Shan and between 0.5 and 1 mm/year further west, with a gradual decrease when reaching the northern headwaters of the Min Jiang watershed, adjacent to the beginning of the Tibetan Plateau. The spatialdistribution of denudation inferred from the various methods we use suggests that most of the differential uplift in the Central Longmen Shan is accommodated by the Beichuan Fault and frontal structures located in the foothills. The denudation pattern seems also to reflect the large-scale propagation of erosion from the Sichuan Basin toward the Plateau. This suggests that the Longmen Shan range is submitted to the combined influences of slow thrusting activity on the frontal structures and progressive westward regressiveerosion as a probable response to a pulse of uplift of the Tibetan PlateauEasternmargin, that started 10 Ma ago.
ISSN: 0040-1951

hal-01564192
https://hal.archives-ouvertes.fr/hal-01564192
DOI : 10.1016/j.tecto.2009.10.026

Éditeur(s) : HAL CCSD Elsevier
Résumé : International audience
Primary hepatocyte cultures are now considered as convenient models for in vitro analyzing liver drug transport. However, if primary human and rat hepatocytes have been well-characterized with respect to drug transporter expression and regulation, much less is known for primary mouse hepatocytes. The present study was therefore designed to gain insights about this point. The profile of sinusoidal and canalicular drug transporter mRNA expression in short time (4h)-cultured mouse hepatocytes was found to be highly correlated with that of freshly isolated hepatocytes; by contrast, those of counterparts cultured for a longer time (until 4days) either in monolayer configurations on plastic or collagen or in sandwich configuration with matrigel were profoundly altered: uptake drug transporters such as Oct1, Oatps and Oat2 were thus down-regulated, whereas most of efflux transporters such as Mdr1a/b, Mrp3, Mrp4 and Bcrp were induced. Moreover, short time-cultured hepatocytes exhibited the highest levels of sinusoidal influx transporter activities. Transporter-mediated drug secretion into canalicular networks was however only observed in sandwich-cultured hepatocytes. Mouse hepatocytes cultured either in monolayer or sandwich configurations were finally shown to exhibit up-regulation of referent transporters in response to exposure to prototypical activators of the drug sensing receptors pregnane X receptor, aryl hydrocarbon receptor or constitutive androstane receptor. Taken together, these data demonstrate the feasibility of using primary mouse hepatocytes for investigating potential interactions of xenobiotics with hepatic transporter activity or regulation, provided that adequate culture conditions are retained.
ISSN: 0928-0987

inserm-00823243
http://www.hal.inserm.fr/inserm-00823243
DOI : 10.1016/j.ejps.2013.01.013
PUBMED : 23396053

Éditeur(s) : HAL CCSD MDPI
Résumé : International audience
Microbial pollution in aquatic environments is one of the crucial issues with regard to the sanitary state of water bodies used for drinking water supply, recreational activities and harvesting seafood due to a potential contamination by pathogenic bacteria, protozoa or viruses. To address this risk, microbial contamination monitoring is usually assessed by turbidity measurements performed at drinking water plants. Some recent studies have shown significant correlations of microbial contamination with the risk of endemic gastroenteresis. However the relevance of turbidimetry may be limited since the presence of colloids in water creates interferences with the nephelometric response. Thus there is a need for a more relevant, simple and fast indicator for microbial contamination detection in water, especially in the perspective of climate change with the increase of heavy rainfall events. This review focuses on the one hand on sources, fate and behavior of microorganisms in water and factors influencing pathogens' presence, transportation and mobilization, and on the second hand, on the existing optical methods used for monitoring microbiological risks. Finally, this paper proposes new ways of research.
ISSN: 1660-4601

hal-01122209
https://hal-univ-rennes1.archives-ouvertes.fr/hal-01122209
PUBMED : 24747537
DOI : 10.3390/ijerph110404292
PUBMEDCENTRAL : PMC4025003

Éditeur(s) : HAL CCSD Elsevier
Résumé : International audience
The dating of alluvial landforms by cosmogenic nuclides requires distinguishing the pre-deposition inheritance from the post-deposition history of the clasts in the studied marker. Moreover, estimating catchment-scale erosion rates from the concentrations of cosmogenic nuclides in active alluvia requires a good knowledge of the local/regional relationships between rock exhumation and transport through space and time. This is still poorly known for timescales of tens of thousand years. In order to document the evolution of clast exhumation and transport rates through time, we analyze in situ 10Be concentrations in boulders and cobbles from hillslopes to outlet of an arid mountainous catchment located in Gobi-Altay, Mongolia, strongly affected by global climatic changes during the Pleistocene-Holocene period. Samples were collected on bedrock, abandoned alluvial deposits, active colluvia and alluvia. Our results show a large 10Be scattering in the active river bed, consistent with a low and discontinuous catchment erosion rate dominated by mass wasting and fluvial incision. On the contrary, pre-exposure signal within abandoned terraces is much more homogeneous, consistent with climatic pulses responsible of strong erosional events on hillslopes and rapid fluvial transport. These results show that exhumation/transport processes at the catchment scale vary in style and intensity through time as a consequence of climatic oscillations. The occurrence of abrupt climatic changes during short periods of time recorded by 10Be concentrations in abandoned alluvia raise questions about the temporal applicability of catchment erosion rates derived from cosmogenic nuclide concentrations measured in sediments of active rivers. On the other hand, strong and short erosion events limit and homogenize the pre-exposure 10Be signal in associated deposits like debris-flows, making them particularly suitable markers for dating in active tectonic and paleoclimatic studies.
ISSN: 0169-555X

hal-00626702
https://hal.archives-ouvertes.fr/hal-00626702
https://hal.archives-ouvertes.fr/hal-00626702/document
https://hal.archives-ouvertes.fr/hal-00626702/file/GEOMOR-2485R1_corrected.pdf
DOI : 10.1016/j.geomorph.2011.07.023

Éditeur(s) : Elsevier Science Bv
Résumé : In this study, the Capbreton canyon head, just off the coast, is investigated using high-resolution multibeam bathymetry datasets, sediment samples and numerical modeling. The HR bathymetry analysis reveals a morphological connection between the longshore trough and the head of the canyon. The analysis of recent sediment samples shows a clear correlation between the sediment of the canyon head and that of the nearshore. Hydrodynamic modeling (a coupled wave-flow model) shows that for high-energy waves, the rotational nature of surf-zone circulation reverses and wave-induced currents have the potential to transport large quantities of nearshore sands toward the canyon head. All these arguments support the assumption that the canyon head captures a part of the sand transported by longshore drift. Over the 15 years of observation (1998–2013), time-lapse bathymetry shows that the floor of the canyon head and one lateral gully network experienced significant morphological reworking. In terms of hazards, despite this strong activity, the position of the canyon head and the profile of its longitudinal slope remained stable and appears as a comforting factor. However, the activity of unusual lateral erosions needs to be monitored.
Marine Geology (0025-3227) (Elsevier Science Bv), 2014-05 , Vol. 351 , P. 1-12

Droits : 2014 Elsevier B.V. All rights reserved.
http://archimer.ifremer.fr/doc/00180/29118/27524.pdf
DOI:10.1016/j.margeo2014.03.001
http://archimer.ifremer.fr/doc/00180/29118/

Éditeur(s) : HAL CCSD Royal Society of Chemistry
Résumé : International audience
Abstract View references (60)Here we show the long term performance at high temperatures of a multi-tube module containing 8 membranes in 4 parallel lines with a total of 545 cm 2 area. The membranes were prepared via thin film dip coating of cobalt oxide silica (CoO xSi) sol-gel on tubular alumina supports. A preliminary study found that the sol-gel containing 20 mol% cobalt oxide formed the best microporous structure with the highest surface area and pore volume. All resulting membranes delivered permeances of ∼1 × 10 -7 mol m -2 s -1 Pa -1 at 500 °C, indicating a high repeatability for the membrane fabrication process. The permselectivities of helium (He) and hydrogen (H 2) over carbon dioxide (CO 2) and nitrogen (N 2) increased from 10-20 at 100 °C to values close to 1000 at 500 °C. Additionally, the apparent energies of activation (E act) for the smaller kinetic diameter gases He and H 2 at 12.2 and 19.5 kJ mol -1 were high and contrary to the negative values for larger gases N 2 and CO 2 at -1.8 and -7.4 kJ mol -1. These remarkable results were attributed to the molecular sieving mechanism of the microporous silica which was enhanced by the embedding of cobalt oxide into the matrix, delivering structural control with an average pore size of 3 Å. The E act for H 2 permeance was higher than that of He, indicating that the cobalt oxide played an important role in H 2 transport. Two membrane lines performed exceptionally well for binary gas mixture processing with H 2 purity reaching values close to 100% in the permeate stream for argon (Ar) concentrations of up to 80% in the retentate stream. A major finding here is that the binary gas selectivity was independent of temperature, contrary to the permselectivity observed for single gas permeance. Further, the H 2 flow rate was greatly affected by the concentration of Ar in the mixture, while the temperature dependency played only a marginal role. In particular, competitive adsorption in the percolative pathways containing pore constrictions or bottlenecks of the anisotropic CoO xSi matrix allowed Ar to impede H 2 diffusion. Finally, the CoO xSi membranes proved thermally stable and robust for 2000 h of testing for various thermal cycles up to 500 °C. © The Royal Society of Chemistry 2012.
Energy & Environmental Science

hal-01683199
https://hal.univ-antilles.fr/hal-01683199
DOI : 10.1039/c2ee03247c

Éditeur(s) : Université des Antilles Études caribéennes
Résumé : L’organisation et la gestion des espaces publics en milieu urbain sont au centre des préoccupations de la puissance publique. Cet intérêt est lié dans les pays en développement aux nombreux dysfonctionnements recensés dans la ville. Ces difficultés sont entretenues par la démographie galopante, l’urbanisation informelle et à l’accroissement des activités industrielles, commerciales entre autres. À Dakar, la question de la mobilité urbaine est exacerbée par les puissants mouvements pendulaires entre la ville centrale et la périphérie amplifiés par les stationnements anarchiques et par l’inorganisation des modes de transports urbains.Le type d’espace public choisi pour cet article est une gare routière située au cœur du Plateau de Dakar : Petersen. Elle apparaît comme l’une des plus importantes gares routières du pays, surtout en matière de fréquentation avec plus de 50 000 personnes par jour. Le mode de gestion appliqué ici est la concession, une convention par laquelle la puissance publique confie la gestion de l’équipement à la SAGES une structure privée prenant en charge les intérêts parfois très contradictoires des différents acteurs.
The organization and management of public spaces in urban areas is a central concern of public authorities. This interest is linked in developing countries with numerous shortcomings identified in the city. These difficulties sustained by massive population growth, urbanization informal, and increased industrial activities, commercial and others. In Dakar, the question of urban mobility is exacerbated by the significant commuting between the city center and the periphery that are amplified by the chaotic parking lots and the disorganization of urban transport modes.The type of public space chosen for this article is a bus station in the heart of the Plateau de Dakar: Petersen. It appears as one of the largest bus stations in the country, especially in terms of attendance with over 50,000 people per day.The management method applied here is the concession, an agreement by which the public entrusts the management of equipment at the SAGES, a private organization that supports some very conflicting interests of different actors.
Sénégal
Dakar

Droits : info:eu-repo/semantics/openAccess
urn:doi:10.4000/etudescaribeennes.7858
http://journals.openedition.org/etudescaribeennes/7858

Éditeur(s) : HAL CCSD Wiley-Blackwell
Résumé : International audience
The Lanzo peridotite massif is a fragment of oceanic lithosphere generated in an ocean-continent transition context and eclogitized during alpine collision. Despite the subduction history, the massif has preserved its sedimentary oceanic cover, suggesting that it may have preserved its oceanic structure. It is an exceptional case for studying the evolution of a fragment of the lithosphere from its oceanization to its subduction and then exhumation. We present a field and petrological study retracing the different serpentinization episodes and their impact on the massif structure. The Lanzo massif is composed of slightly serpentinized peridotites (<20% serpentinization) surrounded by an envelope of foliated serpentinites (100% serpentinization) bordered by oceanic metabasalts and metasedimentary rocks. The limit between peridotites and serpentinites defines the front of serpentinization. This limit is sharp: it is marked by the presence of massive serpentinites (80% serpentinization) and, locally, by dykes of metagabbros and mylonitic gabbros. The deformation of these gabbros is contemporaneous with the emplacement of the magma. The presence of early lizardite in the peridotites testifies that serpentinization began during the oceanization, which is confirmed by the presence of meta-ophicarbonates bordering the foliated serpentinite envelope. Two additional generations of serpentine occur in the ultramafic rocks. The first is a prograde antigorite that partially replaced the lizardite and the relict primary minerals of the peridotite during subduction, indicating that serpentinization is an active process at the ridge and in the subduction zone. Locally, this episode is followed by the deserpentinization of antigorite at peak P-T (estimated in eclogitized metagabbros at 2-2.5 GPa and 550-620 °C): it is marked by the crystallization of secondary olivine associated with chlorite and/or antigorite and of clinopyroxene, amphibole and chlorite assemblages. A second antigorite formed during exhumation partially to completely obliterating previous textures in the massive and foliated serpentinites. Serpentinites are an important component of the oceanic lithosphere generated in slow to ultraslow spreading settings, and in these settings, there is a serpentinization gradient with depth in the upper mantle. The seismic Moho limit could correspond to a serpentinization front affecting the mantle. This partially serpentinized zone constitutes a less competent level where, during subduction and exhumation, deformation and fluid circulation are localized. In this zone, the reaction kinetics are increased and the later steps of serpentinization obliterate the evidence of this progressive zone of serpentinization. In the Lanzo massif, this zone fully recrystallized into serpentinite during alpine subduction and collision. Thus, the serpentinite envelope represents the oceanic crust as defined by geophysicists, and the sharp front of serpentinization corresponds to an eclogitized seismic palaeo-Moho.
ISSN: 0263-4929

hal-00823051
https://hal.archives-ouvertes.fr/hal-00823051
DOI : 10.1111/jmg.12008

Éditeur(s) : Public Library Science
Résumé : The European Union and other states are moving towards Ecosystem Based Fisheries Management to balance food production and security with wider ecosystem concerns. Fishing is only one of several sectors operating within the ocean environment, competing for renewable and non-renewable resources that overlap in a limited space. Other sectors include marine mining, energy generation, recreation, transport and conservation. Trade-offs of these competing sectors are already part of the process but attempts to detail how the seas are being utilised have been primarily based on compilations of data on human activity at large spatial scales. Advances including satellite and shipping automatic tracking enable investigation of factors influencing fishers' choice of fishing grounds at spatial scales relevant to decision-making, including the presence or avoidance of activities by other sectors. We analyse the determinants of English and Welsh scallop-dredging fleet behaviour, including competing sectors, operating in the eastern English Channel. Results indicate aggregate mining activity, maritime traffic, increased fishing costs, and the English inshore 6 and French 12 nautical mile limits negatively impact fishers' likelihood of fishing in otherwise suitable areas. Past success, net-benefits and fishing within the 12 NM predispose fishers to use areas. Systematic conservation planning has yet to be widely applied in marine systems, and the dynamics of spatial overlap of fishing with other activities have not been studied at scales relevant to fisher decision-making. This study demonstrates fisher decision-making is indeed affected by the real-time presence of other sectors in an area, and therefore trade-offs which need to be accounted for in marine planning. As marine resource extraction demands intensify, governments will need to take a more proactive approach to resolving these trade-offs, and studies such as this will be required as the evidential foundation for future seascape planning.
Plos One (1932-6203) (Public Library Science), 2015-01-27 , Vol. 10 , N. 1 , P. 1-14

Droits : 2015 Tidd et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
http://archimer.ifremer.fr/doc/00254/36518/35064.pdf
DOI:10.1371/journal.pone.0116335
http://archimer.ifremer.fr/doc/00254/36518/

Résumé : Chapters in Volume 1: - Law of the sea and international straits - Trends in the French merchant fleet - International shipping - Safety and rescue at sea, the case of yachting - Marine research and economic development - The respective roles played at sea by the state and local authorities - Social trends in populations linked to the sea: commercial seafarers - Future perspectives for marine leisure activities - French ports, outlook for the year 2000 Chapters in Volume 2: - European organisation in terms of seafood - The role of the sea in the development of the DOM/TOM (French overseas départements and territories) - France's international policy and naval and maritime resources - Naval strategies of the major powers - Europe's security in the Mediterranean, the example of the Balkans - Nuclear-weapon-free areas and peace zones at sea - France and geopolitical balances in the South Pacific - The North-west of the Indian Ocean and the world energy supply
Rapports des dix-sept groupes de séminaires de la promotion "Liberté, égalité, fraternité" de l'Ecole nationale d'administration, février à juillet 1988: Chapitres du Tome1: - Le droit de la mer et les détroits internationaux - L'évolution de la flotte de commerce française - Les transports maritimes internationaux - Sécurité et sauvetage en mer, le cas de la plaisance - Recherche en mer et développement économique - Les rôles respectifs de l'État et des collectivités locales en mer - L'évolution sociale des populations attachées à la mer : les marins de commerce - Les perspectives d'avenir des loisirs de la mer - Les ports français, perspectives pour l'an 2000 Chapitres du Tome2: - Organisation européenne en matière de produit de la mer - Le rôle de la mer dans le développement des DOM/TOM - La politique internationale de la France et les moyens navals et maritimes - Les stratégies navales des grandes puissances - La sécurité de l'Europe en Méditerranée, l'exemple des Balkans - Les zones exemptes d'armes nucléaires et les zones de paix en mer - La France et les équilibres géopolitiques dans le Pacifique Sud - Le Nord-Ouest de l'océan Indien et l'approvisionnement énergétique mondial

Droits : info:eu-repo/semantics/openAccess
http://archimer.ifremer.fr/doc/1988/rapport-1027.pdf
http://archimer.ifremer.fr/doc/1988/sup-1027.pdf
http://archimer.ifremer.fr/doc/00000/1027/

Résumé : The carrier ship's deballasting activity in french ports and coastal areas can introduce unwanted or noxious species,with detrimental economical and sociological consequences upon shore based activities ; These activities represent one of the most preeminent sources for employment and incomes along the french coasts ,via coastal fishing,aquaculture,tourism. A ship's ballast water study in sorne french ports has been carried out ,to seek for presence of harmfull or pathogenic organisms.Results let appear pathogenic bacteriae in half of the ships,and weIl known toxic phytoplancton species (from Spain ,north Africa) The technical problem of ballast water treatment is considered.
L'activité de déballastage des navires de transport dans les ports et les eaux côtières françaises est susceptible d'introduire des espèces vivantes indésirables ou néfastes, provenant d'autres régions du globe,avec des conséquences dommageables pour la socio-économie des régions côtières. Cette dernière constitue pour le littoral français une des plus importantes sources d'emplois et de revenus,à travers la pêche côtière,l'aquaculture et le tourisme. Une campagne de prélèvement sur les eaux de ballast de navires en escale dans les ports français a été entreprise,pour rechercher d'éventuels organismes pathogènes ou néfastes. Les résultat font apparaître des bactéries connues comme pathogènes sur la moitié des navires échantillonnés. D'autre part,plusieurs espèces de phytoplancton toxique ont été observées,de provenances diverses(péninsule ibérique,Maghreb). Enfin ,le problème technique du traitement des eaux de ballast est examiné.

Droits : info:eu-repo/semantics/openAccess
http://archimer.ifremer.fr/doc/2000/rapport-6312.pdf
http://archimer.ifremer.fr/doc/00000/6312/

Éditeur(s) : HAL CCSD Public Library of Science
Résumé : International audience
Plant-ants live in a mutualistic association with host plants known as "myrmecophytes" that provide them with a nesting place and sometimes with extra-floral nectar (EFN) and/or food bodies (FBs); the ants can also attend sap-sucking Hemiptera for their honeydew. In return, plant-ants, like most other arboreal ants, protect their host plants from defoliators. To satisfy their nitrogen requirements, however, some have optimized their ability to capture prey in the restricted environment represented by the crowns of trees by using elaborate hunting techniques. In this study, we investigated the predatory behavior of the ant Azteca andreae which is associated with the myrmecophyte Cecropia obtusa. We noted that up to 8350 ant workers per tree hide side-by-side beneath the leaf margins of their host plant with their mandibles open, waiting for insects to alight. The latter are immediately seized by their extremities, and then spread-eagled; nestmates are recruited to help stretch, carve up and transport prey. This group ambush hunting technique is particularly effective when the underside of the leaves is downy, as is the case for C. obtusa. In this case, the hook-shaped claws of the A. andreae workers and the velvet-like structure of the underside of the leaves combine to act like natural Velcro that is reinforced by the group ambush strategy of the workers, allowing them to capture prey of up to 13,350 times the mean weight of a single worker.
ISSN: 1932-6203

hal-00818187
https://hal.archives-ouvertes.fr/hal-00818187
https://hal.archives-ouvertes.fr/hal-00818187/document
https://hal.archives-ouvertes.fr/hal-00818187/file/Dejean2010_Arboreal.pdf
DOI : 10.1371/journal.pone.0011331

Éditeur(s) : HAL CCSD
Résumé : In coastal carbonate aquifers submitted to a saline intrusion, water - rock interactions processes at the interface between freshwater and saltwater are a cause for heterogeneities development within the porous media. The dissolutions and precipitations induced by the mixing zone play a major role in the transport properties of the reservoir and so in the long term evolution of the saltwater intrusion. The characterization and the in situ observation of a current saline intrusion in the Llucmajor carbonate platform at the experimental site of Ses Sitjoles (South-Easth of Mallorca Island, Spain), allowed to study the temporal evolution of the saltwater intrusion and the biogeochemical processes active in the mixing zone. The influence of microstructure, characterized using X-Ray microtomography, on the petrophysical and flow properties was investigated in laboratory and flow-through experiments were also conducted to study the modifications of the microstructures when submitted to water - rock interactions processes. The mixing zone at the Ses Sitjoles site appears as quite thick and not very sensitive to climate and anthropic stress and is proved to be the locus of active microbiological activity. Laboratory studies highlight a strong control of the microporosity on transport processes and of pCO2 on water - rock interactions. The percolation experiments of slightly reactive waters reveal calcite dissolution together with a decrease in permeability due to a rearrangement of micro-grains within the microporous network.
Les processus d'interactions fluide-roche à l'interface eau douce-eau salée, dans les aquifères carbonatés côtiers soumis à une intrusion saline, sont une des causes du développement d'hétérogénéités dans la structure du milieu poreux. Les dissolutions et/ou précipitations engendrées par la présence de cette zone de mélange jouent un rôle déterminant pour les propriétés de transport du réservoir et donc l'évolution à long terme de l'intrusion d'eau salée. En prenant l'exemple de la plateforme carbonatée de Llucmajor au niveau du site expérimental de Ses Sitjoles (Sud-Est de Majorque, Espagne), un travail de caractérisation et d'observation in situ à moyen terme de l'intrusion saline actuelle de ce réservoir permet de décrire son évolution temporelle et les processus biogéochimiques actifs dans la zone de mélange. L'influence de la microstructure du milieu poreux, caractérisée par microtomographie RX, sur les propriétés pétrophysiques et hydrodynamiques est étudiée en laboratoire et des expériences de percolations d'eaux réactives (mélanges eau douce - eau salée et eau pure chargée en CO2) sont réalisées afin d'étudier les modifications de ces microstructures lorsqu'elles sont soumises à des interactions eau - roche. La zone de mélange eau douce - eau salée rencontrée au niveau du site de Ses Sitjoles est large et peu sensible aux contraintes extérieures (climatologiques et anthropiques) et constitue un lieu d'activité microbiologique particulièrement actif. En laboratoire, on observe un fort contrôle des phénomènes de transport par la microporosité et des processus d'interactions eau - roche par la pCO2. Dans le cas d'une percolation de fluides moyennement réactifs, les expériences mettent en évidence une dissolution de calcite accompagnée par une diminution de la perméabilité, liée à un réarrangement des micro-grains à l'intérieur du réseau microporeux.
https://tel.archives-ouvertes.fr/tel-00767152

tel-00767152
https://tel.archives-ouvertes.fr/tel-00767152
https://tel.archives-ouvertes.fr/tel-00767152/document
https://tel.archives-ouvertes.fr/tel-00767152/file/thesegaring.pdf

Éditeur(s) : HAL CCSD Wiley
Résumé : International audience
Organochlorine (OC) pesticides constitute a major class of persistent and toxic organic pollutants, known to modulate drug-detoxifying enzymes. In the present study, OCs were demonstrated to also alter the activity and expression of human hepatic drug transporters. Activity of the sinusoidal influx transporter OCT1 (organic cation transporter 1) was thus inhibited by endosulfan, chlordane, heptachlor, lindane, and dieldrine, but not by dichlorodiphenyltrichloroethane isomers, whereas those of the canalicular efflux pumps MRP2 (multidrug resistance-associated protein 2) and BCRP (breast cancer resistance protein) were blocked by endosulfan, chlordane, heptachlor, and chlordecone; this latter OC additionally inhibited the multidrug resistance gene 1 (MDR1)/P-glycoprotein (P-gp) activity. OCs, except endosulfan, were next found to induce MDR1/P-gp and MRP2 mRNA expressions in hepatoma HepaRG cells; some of them also upregulated BCRP. By contrast, expression of sinusoidal transporters was not impaired (organic anion-transporting polypeptide (OATP) 1B1 and OATP2B1) or was downregulated (sodium taurocholate co-transporting polypeptide (NTCP) and OCT1). Such regulations of drug transporter activity and expression, depending on the respective nature of OCs and transporters, may contribute to the toxicity of OC pesticides.
ISSN: 1095-6670

hal-01063656
https://hal.archives-ouvertes.fr/hal-01063656
DOI : 10.1002/jbt.21543
PUBMED : 24464585

Éditeur(s) : HAL CCSD
Résumé : International audience
The development of diabetic vascular complications is initiated, at least in part, by mitochondrial reactive oxygen species (ROS) production in endothelial cells. Hyperglycemia induces superoxide production in the mitochondria and initiates changes in the mitochondrial membrane potential that leads to mitochondrial dysfunction. Hydrogen sulfide (H2S) supplementation has been shown to reduce the mitochondrial oxidant production and shows efficacy against diabetic vascular damage in vivo. However, the half-life of H2S is very short and it is not specific for the mitochondria. We have therefore evaluated two novel mitochondria-targeted anethole dithiolethione and hydroxythiobenzamide H2S donors (AP39 and AP123 respectively) at preventing hyperglycemia-induced oxidative stress and metabolic changes in microvascular endothelial cells in vitro. Hyperglycemia (HG) induced significant increase in the activity of the citric acid cycle and led to elevated mitochondrial membrane potential. Mitochondrial oxidant production was increased and the mitochondrial electron transport decreased in hyperglycemic cells. AP39 and AP123 (30–300 nM) decreased HG-induced hyperpolarisation of the mitochondrial membrane and inhibited the mitochondrial oxidant production. Both H2S donors (30–300 nM) increased the electron transport at respiratory complex III and improved the cellular metabolism. Targeting H2S to mitochondria retained the cytoprotective effect of H2S against glucose-induced damage in endothelial cells suggesting that the molecular target of H2S action is within the mitochondria. Mitochondrial targeting of H2S also induced >1000-fold increase in the potency of H2S against hyperglycemia-induced injury. The high potency and long-lasting effect elicited by these H2S donors strongly suggests that these compounds could be useful against diabetic vascular complications. © 2016
Pharmacological Research

hal-01373947
https://hal-univ-rennes1.archives-ouvertes.fr/hal-01373947
DOI : 10.1016/j.phrs.2016.08.019
PUBMED : 27565382

Éditeur(s) : HAL CCSD American Physiological Society
Résumé : International audience
Zidi-Yahiaoui N, Callebaut I, Genetet S, Le Van Kim C, Cartron JP, Colin Y, Ripoche P, Mouro-Chanteloup I. Functional analysis of human RhCG: comparison with E. coli ammonium transporter reveals similarities in the pore and differences in the vestibule. Am J Physiol Cell Physiol 297: C537–C547, 2009. First published June 24, 2009; doi:10.1152/ajpcell.00137.2009.—Rh glycoproteins are members of the ammonium transporter (Amt)/methylamine permease (Mep)/Rh family facilitating movement of NH3 across plasma membranes. Homology models constructed on the basis of the experimental structures of Escherichia coli AmtB and Nitrosomonas europaea Rh50 indicated a channel structure for human RhA (RhAG), RhB (RhBG), and RhC (RhCG) glycoproteins in which external and internal vestibules are linked by a pore containing two strictly conserved histidines. The pore entry is constricted by two highly conserved phenylalanines, “twin-Phe.” In this study, RhCG function was investigated by stopped-flow spectrofluorometry measuring kinetic pH variations in HEK293E cells in the presence of an ammonium gradient. The apparent unitary NH3 permeability of RhCG was determined and was found to be close to that of AmtB. With a site-directed mutagenesis approach, critical residues involved in Rh NH3 channel activity were highlighted. In the external vestibule, the importance of both the charge and the conformation of the conserved aspartic acid was shown. In contrast to AmtB, individual mutations of each phenylalanine of the twin-Phe impaired the function while the removal of both resulted in recovery of the transport activity. The impact of the mutations suggests that, although having a common function and a similar channel structure, bacterial AmtB and human Rh vary in several aspects of the NH3 transport mechanisms.
ISSN: 0363-6143

hal-00417492
https://hal.archives-ouvertes.fr/hal-00417492
DOI : 10.1152/ajpcell.00137.2009.

Éditeur(s) : HAL CCSD Elsevier
Résumé : International audience
Recent sedimentary facies and processes along the offshore slope of east Taiwan are investigated using a large set of geophysical and sedimentological data. The Taiwan orogen is often considered as one of the most tectonically active regions in the world and also suffers important climatic activity with an average of four typhoons per year. We have mapped in details the morphosedimentary features and characterized the sedimentary facies along offshore eastern Taiwan. There, we show that the slope is driven by a variety of erosional processes from mass wasting to turbidity current. Mass movements such as slides or mass transport deposits (MTDs) are ubiquitous and affect the whole east coast off Taiwan. Detailed core investigations, such as grain size analysis, chemical and mineralogical composition, revealed that turbidite facies range from thin fine-grained turbidites to thick massive turbidite facies. The detailed analysis of turbidite beds allow us to discuss the controlling factors of turbidity current generation. Turbidity currents represent an important sedimentary process that governs the slope morphology off east Taiwan. Triggering mechanisms likely range from slope instabilities related to earthquakes shaking to failure relative to climatic-controlled pulses of sediment supply. We propose that at least two end-members are characteristics in our turbidite records in term of controlling factor:1) Turbidity currents likely generated by tectonic activity and earthquakes shaking in particular; and2) Turbidity currents likely generated by climatic activity such as typhoon-induced floods.
ISSN: 0025-3227

hal-01277768
https://hal.archives-ouvertes.fr/hal-01277768
DOI : 10.1016/j.margeo.2015.05.013

Éditeur(s) : Scientific Technical Research Council Turkey-tubitak
Résumé : In 2002, the Bosphorus outlet was mapped using an EM 300 multibeam echo-sounder together with a Chirp sonar system. This survey, carried out on board the Ifremer RV 'Le Suroit' in the frame of the BlaSON project, completes the data previously acquired directly at the mouth of the Bosphorus by Di Iorio et al. (1999) in the frame of a NATO SACLANT Undersea Research project using jointly the NATO RV Alliance, and the Turkish Navy Survey ship 'Cubuklu'. This acoustic imaging has identified a canyon system at the slope and a shallow marine fan, which contains shelf incisions extending the Istanbul Strait incision. Multibeam bathymetry, ultra-high resolution seismic profiling and coring correlations on this subaqueous fan area allowed reconstruction of morphology and patterns of sediment distribution indicative of high energy sediment transport processes. The discovery of a shallow water/shelf type fan directly off shore from the Bosphorus and connected to its outlet is consistent with the theories of sudden discharges of large volumes of water. Age dating obtained at the bottom of this subaqueous shelf fan yielded an age of 6700 yr C-14 BP (uncorrected age) for the first marine mollusc encountered at the base. This is in accordance with a last and abrupt reconnection of the Marmara Sea to the Black Sea. A detailed morphological map of the shelf and slope along with seismic profile interpretation and core correlation is presented here. A synthesis is proposed to explain the formation of this subaqueous fan and its relationship with the last connection between Black and Marmara seas aft er the Last Glacial Maximum. This interpretation can be summarized as follows: stage A corresponds to the first erosion surface seen on the shelf related to the Last Glacial Maximum low stand; stage B is the ravine surface onlapping to ca. -30, -40 m; stage C is a second erosional surface related to a sea level fall and eroding most of underlying Unit 1B; and stage D corresponds to the onset of the fan deposit during a period of high water run-off from the Black Sea entering from the Bosphorus. Avulsion branches show that this fan has been active for a long time.
Turkish Journal Of Earth Sciences (1300-0985) (Scientific Technical Research Council Turkey-tubitak), 2011 , Vol. 20 , N. 3 , P. 287-305

Droits : 2011 TÜBİTAK
http://archimer.ifremer.fr/doc/00036/14676/13677.pdf
http://archimer.ifremer.fr/doc/00036/14676/

Éditeur(s) : HAL CCSD
Résumé : International audience
The Jaca basin is a thrust-sheet top basin transported southward andunderlain by basement and cover thrusts separated by a Triassic décollement.Basin-fill upper Cretaceous to lower Oligocene synorogenic sequencesrecord a southward depocenter migration, while growth stratadate a shift of deformation from the mid-Eocene in the N to the earlyMiocene in the S (S-Pyrenean front). However, the deep geometry andkinematic relationships between the cover and basement thrusts remainnon-consensual. We present a new structural and kinematic interpretationfor the eastern Jaca basin, based on subsurface structural maps andcross-sections constructed using industrial seismic reflection profiles.Raman Spectroscopy of Carbonaceous Material (RSCM), vitrinite reflectanceand apatite fission track (AFT) thermochronology data givefurther constraints for the restoration of the deformation and burialexhumationsequence. We define 3 main basement thrusts under thenorthern part of the basin, from N to S the Broto, Fiscal and Guargathrusts. The study also clarifies the deep structure of the cover thrustsand their links with these basement thrusts and those located more tothe north in the Axial Zone (AZ), which altogether define the timing ofthe basement thrust sequence as follows : the Lakoura-Eaux Chaudesthrust system (northern AZ) active up to the end Bartonian, the Gavarnie(central AZ) and Broto thrusts during the Priabonian-Rupelian andthe Fiscal and Guarga thrusts during the late Oligocene-early Miocene.AFT results in the basin fill and AZ date the exhumation of the basinto the late Oligocene-early Miocene, in relation to the Fiscal-Guargathrusting. AFT partial reset argues for a maximum burial < 6 km forthe base of the Eocene detrital sequence (Hecho Group turbidites). Bycontrast, RSCM and vitrinite thermometry at the same level indicatesTmax around 180°C, locally up to 240°C, attesting to a temperature anomalypossibly related to transient fluid circulation.
24 ème Réunion des sciences de la Terre 2014
Pau, France

insu-01080836
https://hal-insu.archives-ouvertes.fr/insu-01080836

Éditeur(s) : HAL CCSD
Résumé : International audience
Gevokizumab is a potent anti-interleukin (IL)-1 beta neutralizing monoclonal antibody (mAb), which may be used for treating inflammatory or autoimmune diseases. The present study was designed to characterize the potential effects of this mAb towards well-established IL-1 beta-mediated repression of hepatic drug detoxifying proteins, like cytochrome P450 (CYP) 3A4 and drug transporters. Primary cultured human hepatocytes were exposed to various concentrations of IL-1 beta in the absence or presence of gevokizumab (5 A mu g/mL); mRNA expression and activity of CYP3A4 and transporters were next determined. Gevokizumab was found to down-modulate, but not abolish, the repression of CYP3A4 and drug transporter mRNAs caused by IL-1 beta in human hepatocytes, through shifting up IL-1 beta half maximal inhibitory concentration (IC50) values by factors ranging from 6.8 to 10.4. The mAb concomitantly shifted IL-1 beta IC50 values towards CYP3A4 activity from 22.0 pg/mL (in the absence of gevokizumab) to 796 pg/mL (in the presence of gevokizumab) and counteracted the decrease of organic anion-transporting polypeptide activity occurring in response to 50 pg/mL IL-1 beta, but not that occurring at higher IL-1 beta concentration (1000 pg/mL). Gevokizumab attenuates, but not abolishes, IL-1 beta-mediated functional repression of CYP3A4 and drug transporters in human hepatocytes, which agrees with the fact that the mAb is considered as a modulator and not a blocker of IL-1 beta signaling. This attenuation of IL-1 beta-mediated down-regulation of hepatic detoxifying proteins by gevokizumab may have to be evaluated in terms of potential therapeutic protein drug-drug interactions when considering future development and therapeutic uses of this IL-1 beta neutralizing mAb.
ISSN: 0378-7966

hal-01616062
https://hal-univ-rennes1.archives-ouvertes.fr/hal-01616062
DOI : 10.1007/s13318-017-0406-1