Éditeur(s) : HAL CCSD American Geophysical Union (AGU) American Geophysical Union
Résumé : International audience
Environmental magnetism of speleothems is still in its early stage of development. Here we report on our investigation of the environmental and paleomagnetic information that has been recorded in speleothems, and what are the factors that control its preservation and reliability. To address these issues, we used a multidisciplinary approach, including rock magnetism, petrography, scanning electron microscopy, stable carbon and oxygen isotope compositions, and major and trace element concentrations. We applied this to a set of samples from different stages of speleothem evolution: present-day dripwater (glass plates), a weathered stalactite, a fresh stalagmite, cave sediments, and terra rossa soils. These samples come from the Penico and Excentricas caves, located in two distinct aquifers of the Algarve region, South Portugal. Our results show that the main magnetic carriers of the speleothems under study are primary (detrital) and consist of maghemite (and magnetite?). Similarities in coercivity and temperature dependence of the studied set of samples suggest that iron oxides are inherited from the terra rossa soils that cap the cave and were transported to the speleothems by dripwater. Hence, they represent a regional environmental signature. Interestingly, a stable and probably detrital remanent magnetization could be isolated in the fresh stalagmite, whereas the weathered stalactite yielded chaotic magnetic directions and very low remanent intensities. We propose that these low intensities can be the result from (i) different remanence acquisition mechanisms between stalagmite and stalactite and/or (ii) iron dissolution by fungal activity. We also suggest that magnetic properties and color and the content in detrital elements in the fresh speleothem inform about environmental processes acting on the interface of rock (soil)-atmosphere, while oxygen isotope composition and alkaline-earth element concentrations inform about calcite-water interaction processes. These results provide a better understanding of how environmental information is recorded in speleothems and what the factors are that control the reliability of the paleomagnetic and paleo-environmental signal.
ISSN: 0148-0227

hal-01114953
https://hal.archives-ouvertes.fr/hal-01114953
DOI : 10.1002/2014JB011381

Éditeur(s) : HAL CCSD Magnolia Press
Résumé : International audience
A new species of the genus Cocconeis is described. Cocconeis sawensis sp. nov. was observed as epiphyte on the macrophyte Chara Linn. sp., collected from a saline lake in Iraq (Sawa Lake). Description of the new species is based on details observed with light and scanning electron microscopy. Cocconeis sawensis belongs to a group of taxa similar to Cocconeis euglypta Ehrenberg, but differs in a number of fine ultrastructural features including stria density and hymenes, raphe, valve shape and rim, valvocopula, hymen perforations and fimbriae shape as well as a presence of a pore-like structure reminiscent of a vestigial raphe in the sternum valve. A comparison with closely related taxa is given.
ISSN: 1179-3155

hal-01296895
https://hal-univ-perp.archives-ouvertes.fr/hal-01296895
DOI : 10.11646/phytotaxa.181.4.2

Éditeur(s) : HAL CCSD Oxford University Press (OUP)
Résumé : International audience
The organogenesis, histogenesis and growth of larvae of the fighting conch Strombus pugilis (Linne, 1758) were studied over a period of 30 d after hatching in laboratory culture. Early development of S. pugilis was examined by light and scanning electron microscopy. Rearing was conducted at 27 +/- 1 degrees C. Veligers were reared at 200 larvae l(-1) in 4-l containers. Larvae were fed with the microalgae Isochrysis galbana and Nanochloropsis oculata at a concentration of 1,000 cell l(-1). The protoconch at hatching measured 212 +/- 12.14 mu m in length and the shell reached 1,100 +/- 29.11 mu m 29 d after hatching. Development characteristics are described from hatching to settlement. Newly hatched veligers possess two velar lobes, a larval shell consisting of 1.5 whorls, eyespots and a single right tentacle. Late veligers (5-d old larvae) have four velar lobes and two shell whorls and the left tentacle appears. Pediveligers show a functional adult heart at 11 d. Crawling behaviour and settlement were observed from 27 to 31 d. Plantigrades were observed after 29 d, when a functioning proboscis is observed and the velar lobes are lost. This study will facilitate the identification of gastropod larval shells in the plankton and of juveniles in the meiobenthos and will aid aquaculture of Strombus species.
ISSN: 0260-1230

hal-01545910
https://hal.archives-ouvertes.fr/hal-01545910
DOI : 10.1093/mollus/eyv011

Éditeur(s) : HAL CCSD Wiley-Blackwell
Résumé : International audience
In order to constrain interpretations of seismic reflection records more effectively, the seismic properties of a middle crustal section exposed in the Saint Barthélémy Massif have been determined. The massif, transected by a 200 m thick shear zone has been systematically sampled for density measurements and modal analysis has been performed in order to define the spatial variations of physical properties. Seismic velocities (Vp, V5, shear wave birefringence), have been measured on five representative samples to 600 MPa and 600°C simultaneously in the three structural directions (X, Y and Z). For two samples, the expep.mental data have been compared with calculated values, based on petrofabric analyses. The Lattice Preferred Orientation (LPO) is determined using universal stage, electron channelling microscopy and neutron diffraction goniometry. Using the experimental and calculated velocity data, we establish a lateral homogeneous anisotropie model.
ISSN: 0954-4879

hal-01389722
http://hal.univ-reunion.fr/hal-01389722
http://hal.univ-reunion.fr/hal-01389722/document
http://hal.univ-reunion.fr/hal-01389722/file/Barruol_aniso_St_Barthelemy_TerraNova1992_hal.pdf
DOI : 10.1111/j.1365-3121.1992.tb00451.x

Éditeur(s) : HAL CCSD Public Library of Science
Résumé : International audience
Among persisting questions on bone calcification, a major one is the link between protein expression and mineral deposition. A cell culture system is here proposed opening new integrative studies on biomineralization, improving our knowledge on the role played by non-collagenous proteins in bone. This experimental in vitro model consisted in human primary osteoblasts cultured for 60 days at the surface of a 3D collagen scaffold mimicking an osteoid matrix. Various techniques were used to analyze the results at the cellular and molecular level (adhesion and viability tests, histology and electron microscopy, RT-and qPCR) and to characterize the mineral phase (histological staining, EDX, ATG, SAED and RMN). On long term cultures human bone cells seeded on the osteoid-like matrix displayed a clear osteoblast phenotype as revealed by the osteoblast-like morphology, expression of specific protein such as alkaline phosphatase and expression of eight genes classically considered as osteoblast markers, including BGLAP, COL1A1, and BMP2. Von Kossa and alizarine red allowed us to identify divalent calcium ions at the surface of the matrix, EDX revealed the correct Ca/P ratio, and SAED showed the apatite crystal diffraction pattern. In addition RMN led to the conclusion that contaminant phases were absent and that the hydration state of the mineral was similar to fresh bone. A temporal correlation was established between quantified gene expression of DMP1 and IBSP, and the presence of hydroxyapatite, confirming the contribution of these proteins to the mineralization process. In parallel a difference was observed in the expression pattern of SPP1 and BGLAP, which questioned their attributed role in the literature. The present model opens new experimental possibilities to study spatio-temporal relations between bone cells, dense collagen scaffolds, NCPs and hydroxyapatite mineral deposition. It also emphasizes the importance of high collagen density environment in bone cell physiology.
ISSN: 1932-6203

hal-01142641
http://hal.upmc.fr/hal-01142641
http://hal.upmc.fr/hal-01142641/document
http://hal.upmc.fr/hal-01142641/file/Silvent_2013_Collagen.pdf
DOI : 10.1371/journal.pone.0057344

Éditeur(s) : HAL CCSD Integrated Ocean Drilling Program
Résumé : International audience
A detailed study of magnetic properties was carried out on ~60 samples from three holes cored during Integrated Ocean Drilling Program Expedition 324, Shatsky Rise. The measurements include scanning electron microscopy (SEM), hysteresis, and first-order reversal curve (FORC) diagrams, as well as measurements of susceptibility at high and low temperatures and variation of saturation isothermal magnetization at low temperature. SEM measurements show the presence of a complex mixture of Fe-Ti oxides with various Ti content x, which is confirmed by the measurements of susceptibility variation with temperature and low-temperature measurements. According to FORC diagrams, the magnetization is carried by single-domain to pseudosingle-domain grains.
ISSN: 1930-1014

hal-00795484
https://hal.archives-ouvertes.fr/hal-00795484
DOI : 10.2204/iodp.proc.324.201.2013

Éditeur(s) : HAL CCSD Elsevier
Résumé : International audience
The mycotoxin enniatin B (EnnB) is predominantly produced by species of the Fusarium genera, and often found in grain. The cytotoxic effect of EnnB has been suggested to be related to its ability to form ionophores in cell membranes. The present study examines the effects of EnnB on cell death, differentiation, proliferation and pro-inflammatory responses in the murine monocyte-macrophage cell line RAW 264.7. Exposure to EnnB for 24 h caused an accumulation of cells in the G0/G1-phase with a corresponding decrease in cyclin D1. This cell cycle-arrest was possibly also linked to the reduced cellular ability to capture and internalize receptors as illustrated by the lipid marker ganglioside GM1. EnnB also increased the number of apoptotic, early apoptotic and necrotic cells, as well as cells with elongated spindle-like morphology. The Neutral Red assay indicated that EnnB induced lysosomal damage; supported by transmission electron microscopy (TEM) showing accumulation of lipids inside the lysosomes forming lamellar structures/myelin bodies. Enhanced levels of activated caspase-1 were observed after EnnB exposure and the caspase-1 specific inhibitor ZYVAD-FMK reduced EnnB-induced apoptosis. Moreover, EnnB increased the release of interleukin-1 beta (IL-1β) in cells primed with lipopolysaccharide (LPS), and this response was reduced by both ZYVAD-FMK and the cathepsin B inhibitor CA-074Me. In conclusion, EnnB was found to induce cell cycle arrest, cell death and inflammation. Caspase-1 appeared to be involved in the apoptosis and release of IL-1β and possibly activation of the inflammasome through lysosomal damage and leakage of cathepsin B.
ISSN: 0041-008X

inserm-00871698
http://www.hal.inserm.fr/inserm-00871698
DOI : 10.1016/j.taap.2012.03.014
PUBMED : 22483798

Éditeur(s) : HAL CCSD American Society of Nephrology
Résumé : International audience
Since the recent publication of data showing favorable outcomes for patients with HIV-1 and ESRD, kidney transplantation has become a therapeutic option in this population. However, reports have documented unexplained reduced allograft survival in these patients. We hypothesized that the unrecognized infection of the transplanted kidney by HIV-1 can compromise long-term allograft function. Using electron microscopy and molecular biology, we examined protocol renal transplant biopsies from 19 recipients with HIV-1 who did not have detectable levels of plasma HIV-1 RNA at transplantation. We found that HIV-1 infected the kidney allograft in 68% of these patients. Notably, HIV-1 infection was detected in either podocytes predominately (38% of recipients) or tubular cells only (62% of recipients). Podocyte infection associated with podocyte apoptosis and loss of differentiation markers as well as a faster decline in allograft function compared with tubular cell infection. In allografts with tubular cell infection, epithelial cells of the proximal convoluted tubules frequently contained abnormal mitochondria, and both patients who developed features of subclinical acute cellular rejection had allografts with tubular cell infection. Finally, we provide a novel noninvasive test for determining HIV-1 infection of the kidney allograft by measuring HIV-1 DNA and RNA levels in patients' urine. In conclusion, HIV-1 can infect kidney allografts after transplantation despite undetectable viremia, and this infection might influence graft outcome.
ISSN: 1046-6673

hal-01121764
https://hal-univ-rennes1.archives-ouvertes.fr/hal-01121764
DOI : 10.1681/ASN.2013050564
PUBMED : 24309185
PUBMEDCENTRAL : PMC3904571

Éditeur(s) : HAL CCSD North Carolina State University
Résumé : Few studies have been conducted about relation between cellulose parameters and biomechanical properties of wood in tropical angiosperms species. For this purpose, on 13 trees from 3 species of French Guyana tropical rainforest in a clear active process of restoring verticality, i) growth strains were measured in situ in order to determine the occurrence of tension wood within samples and ii) cellulose structural parameters were estimated on all the samples using X-ray diffraction method. Crystallite size was estimated from the full-width at half-maximum of the Miller index (002) arc diffraction and angle T was measured following Cave's method. Relationships between these parameters and growth stresses were good and the variations between normal and tension wood were significant, i.e. a lower angle T and a larger crystallite size in tension wood. In order to have a good estimation of the microfibril angle in the main layer of the secondary wall for each species, an experimental calibration was done between angle T and microfibril angle observed with scanning electron microscopy.
ISSN: 19302126

hal-01031799
https://hal.archives-ouvertes.fr/hal-01031799
https://hal.archives-ouvertes.fr/hal-01031799/document
https://hal.archives-ouvertes.fr/hal-01031799/file/42-575-1-PB.pdf

Éditeur(s) : HAL CCSD Magnolia Press
Résumé : International audience
The genus Notomicrus Sharp, 1882 is recorded from Guadeloupe for the first time. Examination of recently collected material revealed the presence of four species, of which three are described as new species so far endemic to Guadeloupe. Notomicrus sabrouxi sp. n. is akin to the continental Neotropical species N. gracilipes Sharp, 1882 and N. traili Sharp, 1882. Notomicrus chailliei sp. n. and N. femineus sp. n. are sibling species which diverge from each other by 3.4% of observed distance in cytochrome oxidase 1 sequences. The latter species seems to be comprised of only females, and is suspected to reproduce by obligate parthenogenesis. The fourth species is N. sharpi Balfour-Browne, 1939. These species are morphologically described notably through scanning electron microscopy study, and data on their distribution in the Guadeloupe archipelago and habitat preferences are presented and discussed.
ISSN: 1175-5326

hal-01544477
https://hal.archives-ouvertes.fr/hal-01544477

Éditeur(s) : HAL CCSD Pergamon
Résumé : This paper presents an experimental study of the deformation of Beaucaire marl subjected to drained triaxial tests under a wide range of confining pressure. Several methods of observation have been used: X-ray computed tomography, scanning electronic microscopy, quantitative image analysis of thin sections and mercury porosimetry. The analysis at the grain scale shows the existence of various deformation modes depending on the mean stress level. These modes are classified with reference to the brittle and ductile regimes. In specimens deformed under low confining pressure, macro-cracks are localised in a band more contracting than the rest of the specimen. In specimens deformed under moderate confining pressure, a series of micro-scale shear bands characterised by a grain re-orientation towards the direction of macro-shear is detected in the zones of localised deformation. The material in between the micro-scale shear bands is contracting more than the rest of the specimen in some cases; in other cases, a schistosity is detected also in the rest of the specimen outside the localisation zones. In the specimen deformed under high confining pressure, no localisation occurs, and a schistosity is observable throughout the specimen.
ISSN: 1365-1609

hal-00411208
https://hal.archives-ouvertes.fr/hal-00411208
DOI : 10.1016/j.ijrmms.2007.08.007

Éditeur(s) : HAL CCSD Elsevier
Résumé : International audience
The potential effects of the mineralogical composition of sediment on the degradation of oil buried on sandy beaches were investigated. Toward that purpose, a laboratory experiment was carried out with sandy sediment collected along NW Iberian Peninsula beaches, tar-balls from the Prestige oil spill (NW Spain) and seawater. The results indicate that the mineralogical composition is important for the physical appearance of the oil (tar-balls or oil coatings). This finding prompted a reassessment of the current sequence of degradation for buried oil based on compositional factors. Moreover, the halo development of the oil coatings might be enhanced by the carbonate concentration of the sand. These findings open new prospects for future monitoring and management programs for oiled sandy beaches.
ISSN: 0025-326X

hal-01122779
https://hal.archives-ouvertes.fr/hal-01122779
DOI : 10.1016/j.marpolbul.2014.06.040

Éditeur(s) : HAL CCSD
Résumé : International audience
Seven individuals of a single morphotype of mussels (Bivalvia: Mytilidae) were found attached to a naturally sunken whale intervertebral disk collected in Guadeloupe (Caribbean) at 800 m depth. These specimens resemble small Idas mussels which are found worldwide at cold seeps and hydrothermal vents, and typically harbor ectosymbiotic bacteria on their gills upon which they depend for nutrition. Based on multi-locus gene sequencing, these specimens appear to belong to a new species closely related to two species now included within the genus Terua. Unexpectedly, its closest relatives are found in the Pacific, questioning how this species has reached the Antilles arc. Based on marker gene sequence analysis, electron and fluorescence microscopy, Terua n. sp. harbors two distinct and abundant extracellular bacterial symbionts located between microvilli at the apical surface of host gill epithelial cells. One is a sulfur-oxidizing bacterium similar to the symbionts previously identified in several deep-sea mussels, while the other is related to Colwellia species, a group of cold-adapted heterotrophic bacteria able to degrade organic compounds. This study provides the first evidence for the existence of a dual symbiosis in mussels from whale fall ecosystems in the Caribbean. The evolutionary history of Terua n. sp. and potential role of its Colwellia symbionts are discussed.
Deep Sea Research Part I: Oceanographic Research Papers

hal-01336943
http://hal.upmc.fr/hal-01336943
http://hal.upmc.fr/hal-01336943/document
http://hal.upmc.fr/hal-01336943/file/Duperron_Colwellia_and.pdf
DOI : 10.1016/j.dsr.2016.05.012

Éditeur(s) : HAL CCSD Wiley
Résumé : International audience
The Caribbean bivalves Codakia orbicularis (Linnée, 1758) and C. orbiculata (Montagu, 1808) live in seagrass beds of Thalassia testudinum and harbor intracellular sulfuroxidizing gamma-proteobacteria. These bacterial symbionts fix CO2 via the Calvin Benson cycle and provide organic compounds to the bivalve. During experimentally induced starvation, no reduced sulfur compounds and no organic particle food are available; the symbionts could be considered as the sole nutrient source of the host bivalve. A previous study has shown that the intracellular bacterial population decreased considerably during starvation and that bacterial endosymbionts were not released by the bivalves. In this study, the activity of two lysosomal marker enzymes (acid phosphatase and arylsulfatase) was detected using cytochemical experiments coupled with energy-dispersive X-ray transmission electron microscopy during sulfide and organic particle starvation. The degradation of bacterial endosymbionts began after 2 weeks of starvation in C. orbiculata and after 3 weeks in C. orbicularis. Degradation processes seem to be continuous over several months and could be responsible for the disappearance of the bacterial endosymbionts within the gills during starvation. These data suggest that the host use symbionts as a nutrient source to survive a hunger crisis. The carbon transfer from the symbionts to the host could be flexible and could consist in transfer of organic matter, “milking,” under normal feeding conditions and digestion of the symbionts under starved conditions.
ISSN: 1059-910X

Droits : info:eu-repo/semantics/OpenAccess
hal-01206367
https://hal.univ-antilles.fr/hal-01206367
https://hal.univ-antilles.fr/hal-01206367/document
https://hal.univ-antilles.fr/hal-01206367/file/revised%20version%20to%20be%20submitted%20to%20the%20editor3oct.pdf
DOI : 10.1002/jemt.22458

Éditeur(s) : HAL CCSD Wiley-Blackwell
Résumé : International audience
Six morphotypes of small mussels (Bivalvia: Mytilidae) were found attached to naturally sunken wood collected in the Bohol Sea (Philippines). These specimens are related to the large Bathymodiolus mussels that are found worldwide at cold seeps and hydrothermal vents. In these habitats, the mytilids harbour sulphur- and methane-oxidizing endosymbionts in their gills and depend on the energy and carbon provided by the symbionts. In this study, bacteria associated with the gills of wood-associated mussels are characterized using molecular and microscopic techniques. The existence of bacteria in the lateral zone of gill filaments in all specimens is demonstrated. Comparative analyses of 16S rRNA gene and adenosine 5′-phosphosulphate (APS) reductase gene sequences indicate that the bacteria are closely related to sulphur-oxidizing endosymbionts of Bathymodiolus. FISHs using specific probes confirm that sulphur oxidizers are by far the most abundant, if not the only bacteria present. Electron micrographs displayed mostly extracellular bacteria located between microvilli at the apical surface of host gill epithelial cells all along the lateral zone of each gill filament. In some specimens, occasional occurrence of intracellular bacteria with similar morphology was noted. This study provides the first molecular evidence for the presence of possible thiotrophic symbiosis in sunken wood ecosystems. With their epibiotic bacteria, wood-associated mussels display a less integrated type of interaction than described in their seep, vent and whale fall relatives.
ISSN: 0168-6496

hal-00715244
https://hal.univ-antilles.fr/hal-00715244
DOI : 10.1111/j.1574-6941.2008.00438.x

Éditeur(s) : HAL CCSD Wiley
Résumé : International audience
The embryonic development in Chione cancellata, from fertilization to straighthinge D-stage veliger larva, occurs in 24 h at 251C. Transmission (TEM) and scanning (SEM) electron microscopy show that morphogenetic processes result in a gastrula with two depressions, 4 h after fertilization (T014 h). Two hours later, one depression, located at the animal pole, develops into an open cave, the floor of which becomes the shell field, characterized by scarce microvilli, located below the lower face of the prototrochal pad. The invagination located at the vegetal pole is characterized by regular microvilli and constitutes the digestive tract. At T018 h, the late gastrula differentiates into a typical motile trochophore, possessing a well-developed prototroch delimiting anterior and posterior embryonic regions. At T019h, the shell field, located between the prototroch and the telotroch, appears as a saddle-shaped region with a wrinkled surface extending on both sides of the embryo, establishing bilateral symmetry. From TEM observations, the apical surface of the underlying cells possesses a modified glycocalyx featuring a dense pellicle overlapping the cells of the shell field. At T0112 h, the prototroch is displaced toward the anterior region by outgrowth of the shell material. TEM views show that the wrinkled surface observed with SEM corresponds to the periostracum, attached dorsally to the hinge and ventrally to the cells of the future mantle edge. At T0115 h, an alveolar matrix of proteinaceous material, which could contain calcite crystals, appears between the periostracum and the mantle. At T0124 h, prodissoconch I formation is completed and the D-stage larvae possess a calcified shell. At this stage of development, only one mantle fold is present, representing the outer fold. The periostracumsecreting cells are in contact with the marginal cells of the velum. This functional velum is composed of four bands of cilia. Our TEMobservations confirm previous descriptions of the velum ciliary bands obtained only from SEM analyses, such as described in other bivalves. The apical sense organ and the digestive tract are poorly developed. From observations at the ultrastructural level of shell differentiation in C. cancellata, we propose a new interpretation of shell differentiation, including hinge and ligament for bivalves as opposed to that described for gastropods.
ISSN: 1077-8306

hal-00755283
https://hal.univ-antilles.fr/hal-00755283
DOI : 10.1111/j.1744-7410.2006.00036.x

Éditeur(s) : HAL CCSD Elsevier
Résumé : International audience
In order to estimate the seismic anisotropy of subducted crust, polycrystalline samples of KA1Si(3)O(8) K-lingunite (25% of the total subducted transformed sediments), have been synthesized and deformed under the temperature and pressure conditions of the subducted slabs. Transmission electron microscopy (TEM) characterizations of the recovered samples reveal that the microstructures are clearly dominated by [001] glide involving screw dislocations. For this reason, only {100} could be identified as glide planes, the question of [001] slip on {110} remains open. Few 1/2 < 111 > dislocations were observed gliding on {110} planes, which implies that 1/2 < 111 > {110} is a harder slip system than those involving [001] slip. The occurrence of sub-grain boundaries suggests that diffusion and climb might be active under these conditions.;To assess the texture of polycrystalline K-lingunite, the crystal preferred orientations (CP05) were calculated using visco-plastic self-consistent (VPSC) polycrystalline plasticity model in simple shear using the slip systems identified by TEM. Finally, the seismic properties of K-lingunite aggregates were calculated from the CPO and single crystal elasticity tensor. K-lingunite is predicted to have a high seismic anisotropy, which could combine constructively with one of the stishovite (same proportion as K-lingunite at the transition zone depth ranges).
ISSN: 0031-9201

hal-00534050
https://hal.archives-ouvertes.fr/hal-00534050
DOI : 10.1016/j.pepi.2010.06.013

Résumé : Ce travail porte sur l'inhibition de l'acier C38 en milieu acide HC1 1M par les extraits alcaloïdes issus de 2 plantes : aspidosperma album et geissospermum laeve. Biodégradables et respectueux de l'environnement, les alcaloïdes pourraient être utilisés temporairement pour prévenir la corrosion de l'acier C39 avec des taux d'inhibition supérieurs à ceux décrits dans la littérature. Dans la première partie du travail, l'influence de la concentration, du temps d'immersion et de la température sur le processus de corrosion de l'acier C38 en milieu HC1 1M en absence de polarisation et spectroscopie d'impédance électrochimique. Les analyses de surface effectuées par microscopie électronique a balayage montrent la présence d'une couche constituée d'un mélange d'oxyde de fer incorporant les alcaloïdes et qui conduit à la diminution de la vitesse de corrosion. La deuxième partie de ce travail a été consacrée à l'isolement des alcaloïdes des deux extraits afin de déterminer le ou les alcaloïdes responsables de l'activité inhibitrice et ainsi remonter aux mécanismes d’adsorption. Il a été montré que l'alcaloïde majoritaire des extraits est responsable de l'inhibition
This work is devoted to the corrosion inhibition of C38 steel in 1M HCL acid medium by alkaloids extracts from two plants : aspidosperma album and geissospermum laeve. Biodegradable and environmentally friendly, alkaloids could be used temporarily to prevent the corrosion of C38 steel with inhibition rates higher than those described in the literature. The first part of this work studies the influence of concentration, immersion time and temperature on the corrosion inhibitor by electrochemical measurements : polarization curves and electrochemical impedance spectroscopy. The surface analyses conducted by scanning electron microscopy showed the presence of a layer composed of an iron oxide mixture incorporating alkaloids extracts and which leads to the decrease of the corrosion rate. The second part of this work was devoted to the isolation of alkaloids of two extracts in order to determine the alkaloids responsible of the inhibitory activity and thus to determine the adsorption mechanisms. It was showed that the major alkaloids of two extracts is responsible of the inhibition activity

http://www.theses.fr/2013AGUY0578/document