Éditeur(s) : HAL CCSD
Résumé :  
Wood samples were taken from the upper and lower sides of 21 naturally tilted trees from 18 families of angiosperms in the tropical rain forest in French Guyana. The measurement of growth stresses ensured that the two samples were taken from wood tissues in a different mechanical state: highly tensile stressed wood on the upper side, called tension wood, and lower tensile stressed wood on the lower side, called opposite wood. Eight species had tension wood fibres with a distinct gelatinous layer (G-layer). The distribution of gelatinous fibres varied from species to species. One of the species, Casearia javitensis (Flacourtiaceae), showed a peculiar multilayered secondary wall in its reaction wood. Comparison between the stress level and the occurrence of G-layer indicates that the G-layer is not a key factor in the production of high tensile stressed wood.
IAWA Journal

hal-01032051
https://hal.archives-ouvertes.fr/hal-01032051

Éditeur(s) : HAL CCSD De Gruyter
Résumé : International audience
This study aims at better understanding the respective influences of specific gravity (γ), microfibril angle (MFA), and cell-wall matrix polymers on viscoelastic vibrational properties of wood in axial direction. The wide variations of properties between normal wood (NW) and compression wood (CW) are in focus. Three young bent trees (Picea abies, Pinus sylvestris and Pinus pinaster) that recovered verticality were sampled. Several observed differences between NW and CW were highly significant in terms of anatomical, physical (γ, shrinkage, CIELab colorimetry), mechanical (compressive strength), and vibrational properties. Specific dynamic modulus of elasticity (E'/γ) decreases with increasing MFA, and Young's modulus (E') can be satisfactorily explained by γ and MFA. Apparently, the type of the cell wall polymer matrix is not influential in this regard. The damping coefficient (tanδ) does not depend solely on MFA of NW and CW. The tanδ - E'/γ relationship evidences that, at equivalent E'/γ, the tanδ of CW is approx. 34% lower than that of NW. This observation is ascribed to the more condensed nature of CW lignins, and this is discussed in the context of previous findings in other hygrothermal and time/frequency domains. It is proposed that the lignin structure and the amount and type of extractives, which are both different in various species, are partly responsible for taxonomy-related damping characteristics.
ISSN: 0018-3830

hal-00808062
https://hal.archives-ouvertes.fr/hal-00808062
https://hal.archives-ouvertes.fr/hal-00808062/document
https://hal.archives-ouvertes.fr/hal-00808062/file/Changes_viscoelastic_vibrational_Bremaud_al.pdf
DOI : 10.1515/hf-2011-0186

Éditeur(s) : HAL CCSD Oxford University Press (OUP)
Résumé : International audience
The mechanism for tree orientation in angiosperms is based on the production of high tensile stress on the upper side of the inclined axis. In many species, the stress level is strongly related to the presence of a peculiar layer, called G-layer, in the fibre wall. The structure of G-layer has been recently described as a hydrogel thanks to N2 adsorption-desorption isotherms of supercritically dried samples showing a high mesoporosity (pores size from 2 to 50 nm). This led us to revisit the concept of G-layer that was until now only described from anatomical observation. Adsorption isotherms of both normal wood and tension wood have been measured on six tropical species. Measurements show that mesoporosity is high in tension wood with typical thick G-layer while it is much less with thinner G-layer, sometimes no more than normal wood. The mesoporosity of tension wood species without G-layer is as low as in normal wood. Not depending on the amount of pores, the pore sizes distribution are always centred around 6-12 nm. These results suggest that, among species producing fibres with G-layer, large structural differences of G-layer exist between species
ISSN: 0022-0957

hal-00387861
https://hal.archives-ouvertes.fr/hal-00387861
DOI : 10.1093/jxp/erp133

Éditeur(s) : HAL CCSD Wiley
Résumé : Wood specific gravity (WSG) is a strong predictor of tree performance across environmental gradients. Yet it remains unclear how anatomical elements linked to different wood functions contribute to variation in WSG in branches and roots across tropical forests. [br/][br/]We examined WSG and wood anatomy in white sand, clay terra firme and seasonally flooded forests in French Guiana, spanning broad environmental gradients found throughout Amazonia. We measured 15 traits relating to branches and small woody roots in 113 species representing the 15 most abundant species in each habitat and representative species from seven monophyletic lineages occurring in all habitats. [br/][br/]Fiber traits appear to be major determinants of WSG, independent of vessel traits, in branches and roots. Fiber traits and branch and root WSG increased from seasonally flooded species to clay terra firme species and lastly to white sand species. Branch and root wood traits were strongly phylogenetically constrained. Lineages differed in wood design, but exhibited similar variation in wood structure across habitats. [br/][br/]We conclude that tropical trees can invest differently in support and transport to respond to environmental conditions. Wind disturbance and drought stress represent significant filters driving tree distribution of Amazonian forests; hence we suggest that biophysical explanations should receive more attention.
ISSN: 0028-646X

hal-01195075
https://hal.archives-ouvertes.fr/hal-01195075
PRODINRA : 269680
PUBMED : 24329812

Éditeur(s) : HAL CCSD
Résumé : International audience
The anatomy of tension wood and opposite wood was compared in 21 tropical rain forest trees from 21 species belonging to 18 families from French Guyana. Wood specimens were taken from the upper and lower sides of naturally tilted trees. Measurement of the growth stress level ensured that the two samples were taken from wood tissues in a different mechanical state: highly tensile-stressed wood on the upper side, called tension wood and normally tensile-stressed wood on the lower side, called opposite wood. Quantitative parameters relating to fibres and vessels were measured on transverse sections of both tension and opposite wood to check if certain criteria can easily discriminate the two kinds of wood. We observed a decrease in the frequency of vessels in the tension wood in all the trees studied. Other criteria concerning shape and surface area of the vessels, fibre diameter or cell wall thickness did not reveal any general trend. At the ultrastructural level, we observed that the microfibril angle in the tension wood sample was lower than in opposite wood in all the trees except one (Licania membranacea).
IAWA Journal

hal-00194915
https://hal.archives-ouvertes.fr/hal-00194915
https://hal.archives-ouvertes.fr/hal-00194915/document
https://hal.archives-ouvertes.fr/hal-00194915/file/IAWA2006_RuelleClairBeauchenePrevostFournier_ccsd.pdf
IRD : PAR00001321

Éditeur(s) : HAL CCSD Springer Verlag/EDP Sciences
Résumé :  
On 10 trees from 10 species of French Guyana tropical rainforest in a clear active process of restoring verticality growth strains were measured in situ in order to determine the occurrence of tension wood within samples. Wood specimens were cut in the vicinity of the growth strains measurements in order to measure some mechanical and physical properties. As suspected, tensile growth strains was very much higher in tension wood zone, because longitudinal modulus of elasticity was slightly higher. Longitudinal shrinkage was also much higher in tension wood than in opposite wood.
ISSN: 1286-4560

hal-01032110
https://hal.archives-ouvertes.fr/hal-01032110
DOI : 10.1051/forest:2007027

Éditeur(s) : HAL CCSD Linnean Society of London
Résumé : International audience
The circumscription of Salicaceae has recently been enlarged to include a majority of the species formerly placed in the polyphyletic tropical family Flacourtiaceae. Several studies have reported a peculiar and infrequently formed multilayered structure of tension wood in four of the tropical genera. Tension wood is a tissue produced by trees to restore their vertical orientation, and most studies have focused on trees developing tension wood by means of cellulose-rich, gelatinous fibres, as is known in Populus L. and Salix L. (Salicaceae s.s.). This study aims to determine if the multilayered structure of tension wood is an anatomical characteristic common in other Salicaceae, and if so, how its distribution correlates to phylogenetic relationships. Therefore, we studied the tension wood of 14 genera of Salicaceae as well as two genera of Achariaceae, one genus of Goupiaceae, and one genus of Lacistemataceae, families closely related to Salicaceae or formerly placed in Flacourtiaceae. Opposite wood and tension wood were compared with light microscopy and 3D Laser Scanning Confocal Microscopy. The results indicate that a multilayered structure of tension wood is common in the family except in Salix, Populus, and one of their closest relatives, Idesia polycarpa Maxim. We suggest that tension wood may be a useful anatomical character in understanding phylogenetic relationships in Salicaceae. Further investigation is still needed on the tension wood of several other putatively close relatives of Salix and Populus, in particular Bennettiodendron Merr., Macrohasseltia L.O.Williams and Itoa Hemsl.
ISSN: 0024-4074

hal-01392845
https://hal.archives-ouvertes.fr/hal-01392845
https://hal.archives-ouvertes.fr/hal-01392845/document
https://hal.archives-ouvertes.fr/hal-01392845/file/GhislainEtAl2016_MultylayerTWSalicaceae_Bot_Jsur the_Lin_Soc_HALssfig.pdf
DOI : 10.1111/boj.12471

Éditeur(s) : HAL CCSD Oxford University Press (OUP): Policy B - Oxford Open Option A
Résumé :  
The mechanism for tree orientation in angiosperms is based on the production of high tensile stress on the upper side of the inclined axis. In many species, the stress level is strongly related to the presence of a peculiar layer, called the G-layer, in the fibre cell wall. The structure of the G-layer has recently been described as a hydrogel thanks to N2 adsorption-desorption isotherms of supercritically dried samples showing a high mesoporosity (pores size from 2-50 nm). This led us to revisit the concept of the G-layer that had been, until now, only described from anatomical observation. Adsorption isotherms of both normal wood and tension wood have been measured on six tropical species. Measurements show that mesoporosity is high in tension wood with a typical thick G-layer while it is much less with a thinner G-layer, sometimes no more than normal wood. The mesoporosity of tension wood species without a G-layer is as low as in normal wood. Not depending on the amount of pores, the pore size distribution is always centred around 6-12 nm. These results suggest that, among species producing fibres with a G-layer, large structural differences of the G-layer exist between species.
Journal of Experimental Botany

hal-01032161
https://hal.archives-ouvertes.fr/hal-01032161
DOI : 10.1093/jxb/erp133

Éditeur(s) : HAL CCSD Springer Verlag
Résumé : International audience
Tension wood of Laetia procera (Poepp.) Eichl. (Flacourtiaceae), a neo-tropical forest species, shows a peculiar secondary wall structure, with an alternance of thick and thin layers, while opposite wood of this species has a typical secondary wall structure (S1 + S2 + S3). Samples for the study of microstructural properties were collected upon the estimation of growth stresses in the living tree, in order to analyze the correlation of the former with the latter. Investigation using optical microscopy, scanning electron microscopy and UV microspectrophotometry allowed the description of the anatomy, ultra-structure and chemistry of this peculiar polylaminate secondary wall. In the thick layers, cellulose microfibril angle is very low (i.e., microfibril orientation is close to fibre axis) and cellulose microfibrils are well organized and parallel to each other. In the thin layers, microfibrils (only observable in the inner layer) are less organized and are oriented with a large angle relative to the axis of the cell. Thick layers are lightly lignified although thin layers show a higher content of lignin, close to that of opposite wood secondary wall. The more the wood was under tensile stress, the less the secondary wall was lignified, and lower the syringyl on guaiacyl lignin units' ratio was. The innermost layer of the secondary wall looks like a typical S3 layer with large microfibril angle and lignin occurrence. The interest of this kind of structure for the understanding of stress generation is discussed.
ISSN: 0931-1890

hal-00194923
https://hal.archives-ouvertes.fr/hal-00194923
https://hal.archives-ouvertes.fr/hal-00194923/document
https://hal.archives-ouvertes.fr/hal-00194923/file/Trees_RuelleYoshidaClairThibaut_submitted2006.pdf
DOI : 10.1007/s00468-007-0128-0

Éditeur(s) : HAL CCSD
Résumé : Wood samples were taken from the upper and lower sides of 21 naturally tilted trees from 18 families of angiosperms in the tropical rainforest in French Guyana. The measurement of growth stresses ensured that the two samples were taken from wood tissues in a different mechanical state: highly tensile stressed wood on the upper side, called tension wood and lower tensile stressed wood on the lower side, called opposite wood. Eight species had tension wood fibres with a distinct gelatinous layer (G-layer). The distribution of gelatinous fibres varied from species to species. One of the species, Casearia javitensis (Flacourtiaceae), showed a peculiar multilayered secondary wall in its reaction wood. Comparison between the stress level and the occurrence of the G-layer indicates that the G-layer is not a key factor in the production of high tensile stressed wood.
IAWA Journal

hal-00112579
https://hal.archives-ouvertes.fr/hal-00112579
https://hal.archives-ouvertes.fr/hal-00112579/document
https://hal.archives-ouvertes.fr/hal-00112579/file/IAWA2006_ClairRuelleBeauchenePrevostFournier_ccsd.pdf
IRD : PAR00001136

Éditeur(s) : HAL CCSD
Résumé : International audience
The main biological function of reaction wood is to act as “muscle” for trees, enabling them to control their posture and right stems when they are permanently disturbed from their set-point inclination by gravity or winds. The key property to achieve this function is the development of high mechanical stress during the formation of reaction wood cells, called “maturation strains”. Moreover, this function is basically performed through the asymmetric formation of wood (in quantity or quality) around the tree circumference. This asymmetry enables stems to bend upward or to compensate for the downward bending induced by gravity, as an asymmetric force associated to maturation strains creates a bending moment (i.e. one side of the stem “pulls” the other one). We will discuss the traits (cross sectional size and shape, wood features as G fibers or microfibril angle, eccentric growth even in usual or extreme cases as buttresses, total biomass growth and height of center of mass, stem lean and set-point angle) involved to understand how the posture control works. We will give simple formulas to estimate a “muscle efficiency” from biomechanical modeling. We will give practical methods to estimate all the involved traits, especially maturation strains from direct and indirect measurements, in peripheral functional wood or by retrospective dendrochronological analysis. Then, we will illustrate how tree stem tropisms and especially gravitropism, widely studied by physiologists, are key processes in natural ecosystems, through practical examples from tropical rainforest tree communities and regeneration of European mixed beech forests. Using the previous definition of postural control efficiency, we will show that the cross section size is rapidly a limiting factor, opposing the extreme strategies of flexible small stems, which bend easily but also right themselves quickly, to stiff big stems. Lastly, as wood in living trees ensures general storage, defence, vascular and skeletal functions, we will ask general questions about synergies and trade-offs between posture control and other functions.
Botany 2014 : new frontiers in botany
Boise, United States

hal-01627031
https://hal.archives-ouvertes.fr/hal-01627031

Éditeur(s) : HAL CCSD Springer Verlag
Résumé :  
Growth strains were measured in situ in nine trees of three species from a French Guiana tropical rainforest in a clearly active verticality restoration process. The aim was to detect tension wood within the samples. Wood specimens were cut in the vicinity of the growth strain measurements in order to determine the microfibril angle and some mechanical and physical properties. As suspected, tensile growth strain was much higher in tension wood zones, as shown by the slightly higher longitudinal modulus of elasticity. Conversely, tension wood showed reduced compression strength. Longitudinal shrinkage was much higher in tension wood than in opposite wood. Clear relationships between the microfibril angle and longitudinal properties were noted in comparison (i) with those observed in gymnosperm compression wood and (ii) with expected relationships from the organization of wood fibres cell wall structure.
ISSN: 0043-7719

hal-01032114
https://hal.archives-ouvertes.fr/hal-01032114
DOI : 10.1007/s00226-010-0323-9

Éditeur(s) : HAL CCSD North Carolina State University
Résumé : Few studies have been conducted about relation between cellulose parameters and biomechanical properties of wood in tropical angiosperms species. For this purpose, on 13 trees from 3 species of French Guyana tropical rainforest in a clear active process of restoring verticality, i) growth strains were measured in situ in order to determine the occurrence of tension wood within samples and ii) cellulose structural parameters were estimated on all the samples using X-ray diffraction method. Crystallite size was estimated from the full-width at half-maximum of the Miller index (002) arc diffraction and angle T was measured following Cave's method. Relationships between these parameters and growth stresses were good and the variations between normal and tension wood were significant, i.e. a lower angle T and a larger crystallite size in tension wood. In order to have a good estimation of the microfibril angle in the main layer of the secondary wall for each species, an experimental calibration was done between angle T and microfibril angle observed with scanning electron microscopy.
ISSN: 19302126

hal-01031799
https://hal.archives-ouvertes.fr/hal-01031799
https://hal.archives-ouvertes.fr/hal-01031799/document
https://hal.archives-ouvertes.fr/hal-01031799/file/42-575-1-PB.pdf

Éditeur(s) : HAL CCSD Springer Verlag
Résumé : International audience
Studies on the periodicity of wood formation provide essential data on tree age and factors related to the control of tree growth. The aim of this work was to investigate the cambial phenology and its relation with leaf phenology and climatic seasonality in two tropical rainforest species belonging to the genus Parkia known for having a contrasted leaf phenology. From wood samples collected at 15 day intervals from April 2009 to February 2012 in five trees of both species, we quantified cambial activity by counting layers of cells in cambial zone, enlarging cells zone and wall thickening cells zone from wood prepared anatomical micro sections. In the same time, we observed leaf shedding behaviors in the crown of the studied trees. In both species, cambial activity was significantly reduced during leafless period. However, the cambial activity of P. velutina was not affected by seasonality while in P. nitida, we found a significant positive effect of the dry season on cambial activity. This diminution of activity associated to leaf shedding occurred annually during the dry season in P. nitida whereas the trees were not synchronous in time for P. velutina. On the contrary that is often admitted, the rest cambial period can be independent of environmental restriction such as dry season. Leaf phenology remains the best proxy to estimate cambial activity.
ISSN: 0931-1890

hal-01135166
https://hal.archives-ouvertes.fr/hal-01135166
https://hal.archives-ouvertes.fr/hal-01135166/document
https://hal.archives-ouvertes.fr/hal-01135166/file/Art_Morel_al_Seasonal_variations_phenological_traits_2015.pdf
DOI : 10.1007/s00468-015-1177-4

Éditeur(s) : HAL CCSD
Résumé :  
The anatomy of tension wood and opposite wood was compared in 21 tropical rain forest trees from 21 species belonging to 18 families from French Guyana. Wood secimens were taken from the upper and lower sides of naturally titled trees. Measurement of the growth stress level ensured that the two samples were taken from wood tissues in a different mechanical state: highly tensile-stressed wood on the upper side, called tension wood and normally tensile-stressed wood on the lower side, called opposite wood. Quantitative parameters relating to fibres and vessels were measured on transverse sections of both tension and opposite wood to check if certain criteria can easily discriminate the two kinds of wood. We observed a decrease in the frequency of vessels in the tension wood in all the trees studied. Other criteria concerning shape and surface area of the vessels, fibre diameter or cell wall thickness did not reveal any general trend.At the ultrastructural level, we observed that the microfibril angle in the tension wood sample was lower than in opposite wood in all the trees except one (Licania membranacea).
IAWA Journal

hal-01032149
https://hal.archives-ouvertes.fr/hal-01032149