Éditeur(s) : EDP Sciences
Résumé : The present work was carried out on the hydroelectric reservoir Petit-Saut on the Sinnamary river in French Guiana. Measurements were performed during the wet and dry seasons along a longitudinal gradient, from upstream of the reservoir in two inflow rivers, to the Sinnamary estuary downstream of the dam. Gold extraction has led to a marked increase in suspended matter and total mercury (HgT) in one of the rivers. Dissolved monomethylmercury (MMHg) measured in surface waters were similar for both rivers: 0.03-0.05 ng l(-1) (1.0-1.7% of the dissolved HgT). These results indicate similar methylation efficiency and/or transfer of MMHg into the dissolved fraction of the water column, independently of the amounts of inorganic mercury transported. Dissolved MMHg concentrations in surface waters of the reservoir were similar to those in the rivers, but were more than 10 times higher in deep anoxic waters, up to 0.6 ng l(-1) (20 % of dissolved HgT). The MMHg concentration profiles in the water column suggest that methylation occurs mainly in anoxic waters and sediments in relation with the activity of sulfate reducing bacteria. Dissolved MMHg concentrations measured in the Sinnamary at the base of the dam were still high (0.5 - 0.6 ng l(-1); 20 to 35% of the dissolved HgT).
Journal de Physique IV (1155-4339) (EDP Sciences), 2003-05 , Vol. 107 , P. 327-331

Droits : EDP Sciences
http://archimer.ifremer.fr/doc/2003/publication-5283.pdf
DOI:10.1051/jp4:20030308
http://archimer.ifremer.fr/doc/00000/5283/

Éditeur(s) : HAL CCSD Elsevier
Résumé : International audience
Background : Biological communities are normally composed of a few abundant and many rare species. This pattern is particularly prominent in microbial communities, in which most constituent taxa are usually extremely rare. Although abundant and rare subcommunities may present intrinsic characteristics that could be crucial for understanding community dynamics and ecosystem functioning, microbiologists normally do not differentiate between them. Here, we investigate abundant and rare subcommunities of marine microbial eukaryotes, a crucial group of organisms that remains among the least-explored biodiversity components of the biosphere. We surveyed surface waters of six separate coastal locations in Europe, independently considering the picoplankton, nanoplankton, and microplankton/mesoplankton organismal size fractions.Results : Deep Illumina sequencing of the 18S rRNA indicated that the abundant regional community was mostly structured by organismal size fraction, whereas the rare regional community was mainly structured by geographic origin. However, some abundant and rare taxa presented similar biogeography, pointing to spatiotemporal structure in the rare microeukaryote biosphere. Abundant and rare subcommunities presented regular proportions across samples, indicating similar species-abundance distributions despite taxonomic compositional variation. Several taxa were abundant in one location and rare in other locations, suggesting large oscillations in abundance. The substantial amount of metabolically active lineages found in the rare biosphere suggests that this subcommunity constitutes a diversity reservoir that can respond rapidly to environmental change.Conclusions : We propose that marine planktonic microeukaryote assemblages incorporate dynamic and metabolically active abundant and rare subcommunities, with contrasting structuring patterns but fairly regular proportions, across space and time.
ISSN: 0960-9822

hal-01149637
http://hal.upmc.fr/hal-01149637
http://hal.upmc.fr/hal-01149637/document
http://hal.upmc.fr/hal-01149637/file/Logares_et_al_CurrentBiol_accepted.pdf
DOI : 10.1016/j.cub.2014.02.050

Éditeur(s) : HAL CCSD Wiley
Résumé : International audience
The extracellular signal-regulated kinase (ERK)1/2 mitogen-activated protein (MAP) kinase pathway has been involved in the positive and negative regulation of cell proliferation. Upon mitogen stimulation, ERK1/ERK2 activation is necessary for G1- to S-phase progression whereas when hyperactived, this pathway could elicit cell cycle arrest. The mechanisms involved are not fully elucidated but a kinase-independent function of ERK1/2 has been evidenced in the MAPK-induced growth arrest. Here, we show that p70S6K, a central regulator of protein biosynthesis, is essential for the cell cycle arrest induced by overactivation of ERK1/2. Indeed, whereas MEK1 silencing inhibits cell cycle progression, we demonstrate that active mutant form of MEK1 or MEK2 triggers a G1 phase arrest by stimulating an activation of p70S6K by ERK1/2 kinases. Silencing of ERK1/2 activity by shRNA efficiently suppresses p70S6K phosphorylation on Thr421/Ser424 and S6 phosphorylation on Ser240/244 as well as p21 expression, but these effects can be partially reversed by the expression of kinase-dead mutant form of ERK1 or ERK2. In addition, we demonstrate that the kinase p70S6K modulates neither the p21 gene transcription nor the stability of the protein but enhances the translation of the p21 mRNA. In conclusion, our data emphasizes the importance of the translational regulation of p21 by the MEK1/2-ERK1/2-p70S6K pathway to negatively control the cell cycle progression. J. Cell. Physiol. 229: 903-915, 2014. © 2013 Wiley Periodicals, Inc.
ISSN: 0021-9541

hal-01061238
https://hal.archives-ouvertes.fr/hal-01061238
https://hal.archives-ouvertes.fr/hal-01061238/document
https://hal.archives-ouvertes.fr/hal-01061238/file/MEK12_OveractivationArticle_GUEGAN_JCP_2014.pdf
DOI : 10.1002/jcp.24521

Éditeur(s) : HAL CCSD Public Library of Science
Résumé : International audience
BACKGROUND/AIM: Although it has been hypothesized that muscle metabolism and fatigability could be impaired in sickle cell patients, no study has addressed this issue. METHODS: We compared muscle metabolism and function (muscle microvascular oxygenation, microvascular blood flow, muscle oxygen consumption and muscle microvascular oxygenation variability, which reflects vasomotion activity, maximal muscle force and local muscle fatigability) and the hemorheological profile at rest between 16 healthy subjects (AA), 20 sickle cell-hemoglobin C disease (SC) patients and 16 sickle cell anemia (SS) patients. RESULTS: Muscle microvascular oxygenation was reduced in SS patients compared to the SC and AA groups and this reduction was not related to hemorhelogical abnormalities. No difference was observed between the three groups for oxygen consumption and vasomotion activity. Muscle microvascular blood flow was higher in SS patients compared to the AA group, and tended to be higher compared to the SC group. Multivariate analysis revealed that muscle oxygen consumption was independently associated with muscle microvascular blood flow in the two sickle cell groups (SC and SS). Finally, despite reduced muscle force in sickle cell patients, their local muscle fatigability was similar to that of the healthy subjects. CONCLUSIONS: Sickle cell patients have normal resting muscle oxygen consumption and fatigability despite hemorheological alterations and, for SS patients only, reduced muscle microvascular oxygenation and increased microvascular blood flow. Two alternative mechanisms can be proposed for SS patients: 1) the increased muscle microvascular blood flow is a way to compensate for the lower muscle microvascular oxygenation to maintain muscle oxygen consumption to normal values or 2) the reduced microvascular oxygenation coupled with a normal resting muscle oxygen consumption could indicate that there is slight hypoxia within the muscle which is not sufficient to limit mitochondrial respiration but increases muscle microvascular blood flow.
ISSN: 1932-6203

inserm-00849035
http://www.hal.inserm.fr/inserm-00849035
http://www.hal.inserm.fr/inserm-00849035/document
http://www.hal.inserm.fr/inserm-00849035/file/2013_Waltz_-_Normal_muscle_oxygen_consumption_and_fatigability_in_sickle_cell_patients.pdf
DOI : 10.1371/journal.pone.0052471

Éditeur(s) : HAL CCSD BioMed Central
Résumé : International audience
Background: Myosin II (or Myosin Heavy Chain II, MHCII) is a family of molecular motors involved in the contractile activity of animal muscle cells but also in various other cellular processes in non-muscle cells. Previous phylogenetic analyses of bilaterian MHCII genes identified two main clades associated respectively with smooth/non-muscle cells (MHCIIa) and striated muscle cells (MHCIIb). Muscle cells are generally thought to have originated only once in ancient animal history, and decisive insights about their early evolution are expected to come from expression studies of Myosin II genes in the two non-bilaterian phyla that possess muscles, the Cnidaria and Ctenophora. Results: We have uncovered three MHCII paralogues in the ctenophore species Pleurobrachia pileus. Phylogenetic analyses indicate that the MHCIIa /MHCIIb duplication is more ancient than the divergence between extant metazoan lineages. The ctenophore MHCIIa gene (PpiMHCIIa) has an expression pattern akin to that of ``stem cell markers'' (Piwi, Vasa...) and is expressed in proliferating cells. We identified two MHCIIb genes that originated from a ctenophore-specific duplication. PpiMHCIIb1 represents the exclusively muscular form of myosin II in ctenophore, while PpiMHCIIb2 is expressed in non-muscle cells of various types. In parallel, our phalloidin staining and TEM observations highlight the structural complexity of ctenophore musculature and emphasize the experimental interest of the ctenophore tentacle root, in which myogenesis is spatially ordered and strikingly similar to striated muscle formation in vertebrates. Conclusion: MHCIIa expression in putative stem cells/proliferating cells probably represents an ancestral trait, while specific involvement of some MHCIIa genes in smooth muscle fibres is a uniquely derived feature of the vertebrates. That one ctenophore MHCIIb paralogue (PpiMHCIIb2) has retained MHCIIa-like expression features furthermore suggests that muscular expression of the other paralogue, PpiMHCIIb1, was the result of neofunctionalisation within the ctenophore lineage, making independent origin of ctenophore muscle cells a likely option.
ISSN: 1471-2148

hal-01544486
https://hal.archives-ouvertes.fr/hal-01544486
DOI : 10.1186/1471-2148-12-107

Éditeur(s) : HAL CCSD Elsevier
Résumé : International audience
We present GPS observations in Morocco and adjacent areas of Spain from 15 continuous (CGPS) and 31 survey-mode (SGPS) sites extending from the stable part of the Nubian plate to central Spain. We determine a robust velocity field for the W Mediterranean that we use to constrain models for the Iberia-Nubia plate boundary. South of the High Atlas Mountain system, GPS motions are consistent with Nubia plate motions from prior geodetic studies. We constrain shortening in the Atlas system to <1.5 mm/yr. 95% confidence level. North of the Atlas Mountains, the GPS velocities indicate Nubia motion with respect to Eurasia, but also a component of motion normal to the direction of Nubia-Eurasia motion, consisting of southward translation of the Rif Mountains in N Morocco at rates exceeding 5 mm/yr. This southward motion appears to be directly related to Miocene opening of the Alboran Sea. The Betic Mountain system north of the Alboran Sea is characterized by WNW motion with respect to Eurasia at 1-2 mm/yr. paralleling Nubia-Eurasia relative motion. In addition, sites located in the Betics north of the southerly moving Rif Mountains also indicate a component of southerly motion with respect to Eurasia. We interpret this as indicating that deformation associated with Nubia-Eurasia plate motion extends into the southern Betics, but also that the Betic system may be affected by the same processes that are causing southward motion of the Rif Mountains south of the Alboran Sea. Kinematic modeling indicates that plate boundary geometries that include a boundary through the Straits of Gibraltar are most compatible with the component of motion in the direction of relative plate motion, but that two additional blocks (Alboran-Rif block, Betic Mountain block), independent of both Nubia and Eurasia are needed to account for the motions of the Rif and Betic Mountains normal to the direction of relative plate motion. We speculate that the southward motions of the Alboran-Rif and Betic blocks may be related to mantle flow, possibly induced by southward rollback of the subducted Nubian plate beneath the Alboran Sea and Rif Mountains.
ISSN: 0264-3707

hal-00475538
https://hal.archives-ouvertes.fr/hal-00475538
DOI : 10.1016/j.jog.2009.10.007

Éditeur(s) : HAL CCSD American Society for Cell Biology
Résumé : International audience
Based on its shedding and binding activities, the disintegrin and metalloprotease 12 (ADAM12) has been implicated in cell signaling. Here we investigate the intracellular protein interaction network of the transmembrane ADAM12L variant using an integrative approach. We identify the integrin-linked kinase (ILK) as a new partner for ADAM12L cellular functions. We demonstrate that ADAM12L coimmunoprecipitates with ILK in cells and that its cytoplasmic tail is required for this interaction. In human cultured hepatic stellate cells (HSCs), which express high levels of endogenous ADAM12L and ILK, the two proteins are redistributed to focal adhesions upon stimulation of a β1 integrin-dependent pathway. We show that down-regulation of ADAM12L in HSCs leads to cytoskeletal disorganization and loss of adhesion. Conversely, up-regulation of ADAM12L induces the Akt Ser-473 phosphorylation-dependent survival pathway via stimulation of β1 integrins and activation of phosphoinositide 3-kinase (PI3K). Depletion of ILK inhibits this effect, which is independent of ADAM12L proteolytic activity and involves its cytoplasmic domain. We further demonstrate that overexpression of ADAM12L promotes kinase activity from ILK immunoprecipitates. Our data suggest a new role for ADAM12L in mediating the functional association of ILK with β1 integrin to regulate cell adhesion/survival through a PI3K/Akt signaling pathway.
ISSN: 1059-1524

Droits : info:eu-repo/semantics/OpenAccess
inserm-00717429
http://www.hal.inserm.fr/inserm-00717429
http://www.hal.inserm.fr/inserm-00717429/document
http://www.hal.inserm.fr/inserm-00717429/file/MBoC_E11-11-0918R-A_LEYME_ET_AL.pdf
PUBMED : 22767580

Éditeur(s) : HAL CCSD Nature Publishing Group
Résumé : International audience
Transmembrane CD95L (Fas ligand) can be cleaved to release a promigratory soluble ligand, cl-CD95L, which can contribute to chronic inflammation and cancer cell dissemination. The motility signaling pathway elicited by cl-CD95L remains poorly defined. Here, we show that in the presence of cl-CD95L, CD95 activates the Akt and RhoA signaling pathways, which together orchestrate an allosteric activation of the Na+/H+ exchanger NHE1. Pharmacologic inhibition of Akt or ROCK1 independently blocks the cl-CD95L-induced migration. Confirming these pharmacologic data, disruption of the Akt and ROCK1 phosphorylation sites on NHE1 decreases cell migration in cells exposed to cl-CD95L. Together, these findings demonstrate that NHE1 is a novel molecular actor in the CD95 signaling pathway that drives the cl-CD95L-induced cell migration through both the Akt and RhoA signaling pathways.
ISSN: 2045-2322

hal-01334067
https://hal-univ-rennes1.archives-ouvertes.fr/hal-01334067
https://hal-univ-rennes1.archives-ouvertes.fr/hal-01334067/document
https://hal-univ-rennes1.archives-ouvertes.fr/hal-01334067/file/Cleaved%20FAS%20ligand%20CC-BY.pdf
DOI : 10.1038/srep28008

Éditeur(s) : HAL CCSD Nature Publishing Group
Résumé : International audience
CYLD is a tumour suppressor gene mutated in familial cylindromatosis, a genetic disorder leading to the development of skin appendage tumours. It encodes a deubiquitinating enzyme that removes Lys63- or linear-linked ubiquitin chains. CYLD was shown to regulate cell proliferation, cell survival and inflammatory responses, through various signalling pathways. Here we show that CYLD localizes at centrosomes and basal bodies via interaction with the centrosomal protein CAP350 and demonstrate that CYLD must be both at the centrosome and catalytically active to promote ciliogenesis independently of NF-κB. In transgenic mice engineered to mimic the smallest truncation found in cylindromatosis patients, CYLD interaction with CAP350 is lost disrupting CYLD centrosome localization, which results in cilia formation defects due to impairment of basal body migration and docking. These results point to an undiscovered regulation of ciliogenesis by Lys63 ubiquitination and provide new perspectives regarding CYLD function that should be considered in the context of cylindromatosis.
ISSN: 2041-1723

hal-01063915
https://hal.archives-ouvertes.fr/hal-01063915
DOI : 10.1038/ncomms5585
PUBMED : 25134987

Éditeur(s) : HAL CCSD Elsevier
Résumé : International audience
The deep structure and deformation mechanisms of the Longmen Shan thrust belt (Sichuan, China), at the eastern border of the Tibetan plateau, were largely debated after the devastating Mw 7.9 Wenchuan earthquake (2008). Recent geophysical studies and field investigations have been focused on the active motion of the major Beichuan fault, which ruptured during the earthquake. However, the total exhumation across the fault still remains unclear. In the hanging wall of the Beichuan fault, the South China block is exhumed in the Pengguan massif. Close to the Beichuan fault, the rocks of the Pengguan massif underwent greenschist facies metamorphism associated with brittle-ductile deformation. No metamorphism is observed in the footwall of the fault. In this study, we characterize and date the metamorphic history recorded in the hanging wall of the Beichuan fault in order to constrain the depth and timing of exhumation of the rocks of the Pengguan massif along the fault. A high-resolution petrological approach involving chemical analyses and X-ray maps was used to analyze the micrometric metamorphic minerals. The P-T conditions of the greenschist facies metamorphic event were estimated by an inverse multi-equilibrium thermodynamic approach. The results, 280 ± 30 °C and 7 ± 1 kbar, suggest that the rocks of the Pengguan massif were exhumed from ca. 20 km depth. Our results underline the importance of the thrusting component in the long-term behavior of the Beichuan fault and provide a minimal depth at which the fault is rooted. In situ laser ablation 40Ar/39Ar dating of metamorphic white mica revealed that the greenschist overprint occurred at 135–140 Ma.The Pengguan massif was therefore partially thrusted along the Beichuan fault during the Lower Cretaceous, long before the Eocene-Miocene exhumation phase which is well-constrained by low-temperature thermochronology. Our results provide the first independent depth information for the exhumation history of the Pengguan massif and reveal a previously undocumented Lower Cretaceous tectonic event that marks the onset of the thick-skinned deformation in the external domain of the Longmen Shan (East of the Wenchuan Fault).
ISSN: 0743-9547

hal-01685556
https://hal.archives-ouvertes.fr/hal-01685556
DOI : 10.1016/j.jseaes.2017.04.003

Éditeur(s) : Université des Antilles Études caribéennes
Résumé : Le tourisme et le nautisme ont longtemps été, chacun de leur côté, des leviers du développement territorial des régions littorales et intérieures. Progressivement, le rapprochement institutionnel, fonctionnel et commercial des deux activités a donné naissance à un « tourisme nautique » qui contribue à dynamiser les espaces concernés. L’objectif de cette contribution est de montrer comment, de cette synergie entre les deux domaines, sont nés des produits touristiques à fort pouvoir d’animation et de valorisation des territoires.
For a long time, tourism and water sports have independently played a part in the territorial development of inland and coastal regions. Progressively, the institutional, functional, and commercial merger of the two activities has given birth to “water sports tourism” that contributes to the development of those territories. This paper aims to examine how the synergy between these two sectors has led to the creation of tourism products that highly galvanize and enhance territorial attractiveness.

Droits : info:eu-repo/semantics/openAccess
urn:doi:10.4000/etudescaribeennes.10505
http://journals.openedition.org/etudescaribeennes/10505

Éditeur(s) : HAL CCSD Society for the Study of Reproduction
Résumé : International audience
Transcription factor GATA4 is required for the development and function of the mammalian gonads. We first reported that the GATA4 gene in both human and rodents is expressed as two major alternative transcripts that differ solely in their first untranslated exon (exon 1a vs. exon 1b). We had also showed by quantitative PCR that in mouse tissues, both Gata4 exon 1a- and 1b-containing transcripts are present in all sites that are normally positive for GATA4 protein. In adult tissues, exon 1a-containing transcripts generally predominate. A notable exception, however, is the testis where the Gata4 exon 1a and 1b transcripts exhibit a similar level of expression. We now confirm by in situ hybridization analysis that each transcript is also strongly expressed during gonad differentiation in both sexes in the rat. To gain further insights into how Gata4 gene expression is controlled, we characterized the mouse Gata4 promoter sequence located upstream of exon 1b. In vitro studies revealed that the Gata4 1b promoter is less active than the 1a promoter in several gonadal cell lines tested. Whereas we have previously shown that endogenous Gata4 transcription driven by the 1a promoter is dependent on a proximally located Ebox motif, we now show using complementary in vitro and in vivo approaches that Gata4 promoter 1b-directed expression is regulated by GATA4 itself. Thus, Gata4 transcription in the gonads and other tissues is ensured by distinct promoters that are regulated differentially and independently.
ISSN: 0006-3363

hal-01010626
https://hal-univ-rennes1.archives-ouvertes.fr/hal-01010626
DOI : 10.1095/biolreprod.113.113290
PUBMED : 24352556

Éditeur(s) : HAL CCSD
Résumé : International audience
Antiviral proteins that recognize pathogen-specific or aberrantly located molecular motifs are perfectly positioned to act as pattern-recognition receptors and signal to the immune system. Here we investigated whether the interferon-induced viral restriction factor tetherin (CD317/BST2), which is known to inhibit HIV-1 particle release by physically tethering virions to the cell surface, has such a signaling role. We find that upon restriction of Vpu-defective HIV-1, tetherin acts as a virus sensor to induce NFκB-dependent proinflammatory gene expression. Signaling requires both tetherin's extracellular domain involved in virion retention and determinants in the cytoplasmic tail, including an endocytic motif, although signaling is independent of virion endocytosis. Furthermore, recruitment of the TNF-receptor-associated factor TRAF6 and activation of the mitogen-activated protein kinase TAK1 are critical for signaling. Human tetherin's ability to mediate efficient signaling may have arisen as a result of a five amino acid deletion that occurred in hominids after their divergence from chimpanzees.
ISSN: 1934-6069

hal-01121777
https://hal-univ-rennes1.archives-ouvertes.fr/hal-01121777
PUBMED : 23159053
DOI : 10.1016/j.chom.2012.10.007
PUBMEDCENTRAL : PMC3556742

Éditeur(s) : HAL CCSD Elsevier
Résumé : International audience
3D seismic data located in the Gjallar Ridge (Vøring Basin, offshore Norway) reveals a closely-spaced polygonal fault system affecting more than 800 m of homogeneous mud-dominated Quaternary and Tertiary sequences. As some faults reach the modern seafloor, they represent an active polygonal fault system at present day. Even if the processes remain unclear and are still under debate, it is generally agreed that the initiation of polygonal faults is the result of shallow burial dewatering of fine-grained unconsolidated sediments by volumetric compaction. 3D seismic data are commonly interpreted by propagating horizons automatically and by picking faults manually. However, in the case of polygonal fault intervals, this approach is time consuming due to the huge number of faults and because automatic propagation can be misleading. In this study, we applied a new technique of 3D seismic interpretation based on a sequential stratigraphy analysis, using the new PaleoScan© software (Eliis Company). It allowed us to build a 3D geological model computing more than 300 horizons within the faulted intervals. We then used the coherency attribute, depicting anomalies in the shape of seismic waveform like faults, in order to constrain a possible link between fault distribution and stratigraphic levels. Our approach allows fault throws to be calculated in milliseconds on any polygonal fault plane. The result shows that fault segments have been reactivated by dip-linkage. Distribution of faults depends on mechanical units, intervals characterized by different petrophysical properties, which are independent from lithological and diagenetic changes. According to these results, we propose a model showing the evolution of polygonal fault intervals in which faulting stages are separated by a quiescence phase during burial. A first tier of polygonal faults is initiated at a specific depth, according to the Cam-clay model. Then, following a period of quiescence during which mud-rich sediments continued to accumulate, new fault segments are initiated above the first mechanical unit and within this undeformed interval. New nucleated faults then connect downward to pre-existing underlying polygonal fault system, thus progressively increasing the thickness of the faulted interval.
ISSN: 0025-3227

hal-00772470
https://hal.archives-ouvertes.fr/hal-00772470
DOI : 10.1016/j.margeo.2012.07.016

Éditeur(s) : HAL CCSD
Résumé : Communication between tumoral cells and the microenvironnement plays an essential role in the developpement of tumors. In that context, the new family of metalloproteases, the ADAM proteins, are major regulators of the tumoral progression by acting on the bioavaibility of importants mediators of cellular communication as cytokines and growth factors. Among this family, ADAM12 is the most associated with cancer. It has been shown to mediate signaling pathways by a process independant of its metalloproteasis activity, in particular for TGFβ signaling. This study show for the first time a role for the membrane form of ADAM12 in the induction of epithelial to mesenchymal transition (EMT), a essential process involved in tumor invasion, whom TGFβ is a main inducer. This effect is mediated by the activation of TGFβ signaling pathways, SMAD3, AKT and ERK and require the cytoplasmic tail of ADAM12L but not its catalytic activity. Activation of these pathways could involve a relocalisation of ADAM12L in special signaling platform in lipid rafts. Moreover, we have shown that ADAM12L interact with ZO-1 and ZO-2, two proteins of tight junctions, and could facilitate their desassembling during EMT. This work underscore for the first time a new function of ADAM12L in EMT, by a mecanism invovlving a modulation of signals regulating this process. A better understanding of the dynamic of these molecular mecanisms could allow the developpement of new targeted therapies to fight against tumoral progression.
Les échanges entre les cellules tumorales et le microenvironnement jouent un rôle essentiel dans le développement des tumeurs. Dans ce contexte, la nouvelle famille de métalloprotéases, les protéines ADAM, constituent aujourd’hui des régulateurs majeurs de la progression tumorale en agissant sur la biodisponibilité des médiateurs de la communication cellulaire que sont les cytokines, chimiokines et facteurs de croissance. Au sein de cette famille, ADAM12 est la plus associée au cancer. Elle possède la particularité de jouer un rôle dans la signalisation cellulaire, de façon indépendante de son activité métalloprotéase, notamment dans les voies de signalisation du TGFβ. Notre étude montre pour la première fois un rôle pour la forme membranaire d'ADAM12 dans l'induction de la transition épithélio-mésenchymateuse (EMT), un processus essentiel à l'invasion tumorale dont le TGFβ est un inducteur majeur. Cet effet est médié par l'activation des voies de signalisation du TGFβ, impliquant les protéines SMAD3, AKT et ERK et requiert le domaine cytoplasmique d'ADAM12L mais pas son domaine catalytique. L'activation de ces voies de signalisation pourrait impliquer une relocalisation d'ADAM12L au sein de plates-formes de signalisation dans les radeaux lipidiques. Par ailleurs, nous avons montré qu'ADAM12L interagit avec les protéines ZO-1 et ZO-2, des protéines des jonctions serrées, et pourrait favoriser leur désassemblage au cours de l'EMT. Nos travaux ont permis de mettre en évidence une nouvelle fonction pour ADAM12L dans l'EMT, par un mécanisme impliquant une modulation des signaux régulant ce processus. Une meilleure compréhension de la dynamique de ces mécanismes moléculaires pourrait permettre de développer de nouvelles thérapies ciblées pour lutter contre la progression tumorale.
https://tel.archives-ouvertes.fr/tel-01267577

Droits : info:eu-repo/semantics/OpenAccess
NNT : 2015REN1S063
tel-01267577
https://tel.archives-ouvertes.fr/tel-01267577
https://tel.archives-ouvertes.fr/tel-01267577/document
https://tel.archives-ouvertes.fr/tel-01267577/file/RUFF_Michael.pdf

Éditeur(s) : Université des Antilles Études caribéennes
Résumé : Cette étude démontre que l’écriture du roman The Swinging Bridge constitue une action de récupération de la mémoire féminine indienne qui réactive les liens entre Indo-Trinidadiens du Canada et Indo-Trinidadiens vivant à Trinidad tout en rétablissant une relation avec l’Inde des origines. Elle montre que, dans l’espace du roman, des relations s’établissent entre le centre historique réel ou mythique des établissements dispersés, et des sociétés d’installation par le biais d’une intrigue qui amène personnages et lecteurs à la conscience de former un peuple en diaspora.Elle analyse les procédés littéraires d’une récupération de la mémoire qui révèle, rectifie et réorganise dans une nouvelle cohérence des fragments épars réintroduisant dans la mémoire collective les qualités ancestrales d’indépendance de la femme indienne. Elle révèle le caractère hybride de l’identité culturelle diasporique qui se construit tout au long du roman puis analyse les enjeux pour l’écrivain féminin postcolonial diasporique de cet acte de représentation.Elle conclut que cette représentation constitue une réécriture contre discursive de l’Histoire trinidadienne et qu’elle introduit la définition d’un continuum identitaire indo-américain qui contribue à résorber la dislocation et la fragmentation qui caractérisent cet espace essentiellement diasporique.
This work proposes to read The Swinging Bridge as a process by which a hybrid Indo-Trinidadian Diaspora is created. It argues that the novel’s recovery of memory constitutes a process by which individual memories turn into a collective diasporic memory which re-establishes links between Indo-Trinidadians dispersed in Canada and Trinidad. We demonstrate that rebinding to the Trinidadian space and recovering part of the memory of the Indian historical centre set the three spaces in a diasporic relation.This study also demonstrates that the novel’s narrative semantics rejects cultural conflict, as well as essentialism and creates a hybrid cultural identity. It establishes that the construction of diaspora at work in The Swinging Bridge advocates for the appreciation routes rather than roots and is compatible with the idea of a multi-locational diasporic consciousness.
Caraïbes
Trinité

Droits : info:eu-repo/semantics/openAccess
urn:doi:10.4000/etudescaribeennes.5757
http://journals.openedition.org/etudescaribeennes/5757

Éditeur(s) : HAL CCSD Maison des Sciences de l'Homme
Résumé : National audience
L'archipel antillais comprend (à l'exception de la Guyane) les seuls territoires français des Amériques.Cependant, même au sein de ces territoires (la Martinique et l'archipel de Guadeloupe) l'archéologie professionnelle ne s'est vraiment développée que dans le dernier quart du XXe siècle. C'est à partir de ces " têtes de ponts " que commencent à se développer depuis quelques années des actions en direction d'autres îles de l'archipel. Ainsi, depuis la Martinique, des projets ponctuels ont été développés au début des années 1990 à Trinidad ou à Cuba avec le soutien du ministère français des Affaires étrangères (Rodriguez-Loubet, 1995). Le premier programme pluriannuel a été la mission archéologique " Sud-Dominique ", mise en place en 2005. L'expérience qui en est issue permet de tenter un premier bilan tant au niveau des résultats scientifiques qu'en matière de gestion du patrimoine archéologique à la Dominique et, plus largement, au sein des Petites Antilles anglophones. Nous présenterons les difficultés inhérentes à la mise en place d'un programme archéologique dans de petits états indépendants de fraîche date ne disposant pas d'un outil législatif adéquat. Nous tenterons surtout de montrer l'intérêt qu'il y a, pour les chercheurs français, à sortir de leurs bastions martiniquais et guadeloupéens et envisagerons quelques perspectives concernant l'avenir de la recherche archéologique française au sein de la Caraïbe. Elle semble maintenant disposer d'un certain nombre d'atouts qui devraient lui permettre d'y jouer un rôle majeur.
Les Nouvelles de l'archéologie

hal-00967105
https://hal.univ-antilles.fr/hal-00967105
https://hal.univ-antilles.fr/hal-00967105/document
https://hal.univ-antilles.fr/hal-00967105/file/111-112-be_rard.pdf

Éditeur(s) : HAL CCSD Oldendorf/Luhe
Résumé : International audience
Unmanned aerial vehicles (UAVs) are being increasingly used in studies of marinefauna. Here, we tested the use of a UAV (DJI Phantom II®) to assess fine-scale variation in densitiesof 2 elasmobranchs (blacktip reef sharks Carcharhinus melanopterus and pink whiprays Himanturafai) on reef systems off Moorea (French Polynesia). We flew parallel transects designed to samplereef habitats (fringing, channel and sandflat habitats) across 2 survey blocks. Block 1 included ashark and ray provisioning site with potentially higher elasmobranch densities, whereas Block 2most likely had lower densities with no provisioning activities. Across 10 survey days in July 2014,we flew 3 transects (400 m) within each survey block (n = 60 total transect passes). As expected,densities (animals ha−1) were significantly higher in Block 1 than in Block 2, particularly where provisioningactivities occur. Differences between habitats surveyed were also found. Our study providesthe first direct estimates of shark and ray densities in coral-reef ecosystems and demonstratesthat UAVs can produce important fishery-independent data for elasmobranchs, particularly inshallow-water habitats.
ISSN: 0171-8630

hal-01416757
https://hal-univ-perp.archives-ouvertes.fr/hal-01416757
DOI : 10.3354/meps11945

Éditeur(s) : Elsevier
Résumé : A comprehensive analysis of velocity data from subsurface floats in the northwestern tropical Atlantic at two depth layers is presented: one representing the Antarctic Intermediate Water (AAIW, pressure range 600-1050dbar), the other the upper North Atlantic Deep Water (uNADW, pressure range 1200-2050dbar). New data from three independent research programs are combined with previously available data to achieve blanket coverage in space for the AAIW layer, while coverage in the uNADW remains more intermittent. Results from the AAIW` mainly confirm previous studies on the mean flow, namely the equatorial zonal and the boundary currents, but clarify details on pathways, mostly by virtue of the spatial data coverage that sets float observations apart from e.g. shipborne or mooring observations. Mean transports in each of five zonal equatorial current bands is found to be between 2.7 and 4.5 Sv. Pathways carrying AAIW northward beyond the North Brazil Undercurrent are clearly visible in the mean velocity field, in particular a northward transport of 3.7Sv across 16 degrees N between the Antilles islands and the Mid-Atlantic Ridge. New maps of Lagrangian eddy kinetic energy and integral time scales are presented to quantify mesoscale activity. For the uNADW, mean flow and mesoscale properties are discussed as data availability allows. Trajectories in the uNADW east of the Lesser Antilles reveal interactions between the Deep Western Boundary Current (DWBC) and the basin interior, which can explain recent hydrographic observations of changes in composition of DWBC water along its southward flow. (C) 2009 Elsevier Ltd. All rights reserved.
Deep Sea Research Part I: Oceanographic Research Papers (0967-0637) (Elsevier), 2009-10 , Vol. 56 , N. 10 , P. 1615-1632

Droits : 2009 Elsevier Ltd All rights reserved.
http://archimer.ifremer.fr/doc/2009/publication-6815.pdf
DOI:10.1016/j.dsr.2009.06.002
http://archimer.ifremer.fr/doc/00000/6815/

Éditeur(s) : HAL CCSD BioMed Central
Résumé : International audience
Background: Overweight in Guadeloupe is a public health matter affecting children and adults. In the present study we evaluated the metabolic profile, including serum ghrelin, leptin and adiponectin levels, in normal weight, overweight and obese school children and we analyzed the potential changes in anthropometric and metabolic risk factors after a 1-year lifestyle intervention program. Methods: Parameters were assessed at baseline and at 1 year. Three groups (G) were defined according the International Obesity Task Force reference values, G1: normal weight / G2: overweight / G3: obese. The lifestyle intervention included dietary counseling, regular physical activity and family support. Results: A total of 120 children (G1: n = 44, G2: n = 39, G3: n = 37), aged 11– 15 years and 59 % girls were enrolled. Obese children showed significant lower HDL-C, adiponectin and ghrelin concentrations, higher triglycerides, fasting blood glucose, insulin and leptin levels and also higher frequencies of abdominal obesity (G1: 2.3 %, G2: 28.2 %, G3: 73 %) and insulin resistance (GI: 39 %, G2: 72 %, G3: 89 %) than the other groups. In the overall sample, the linear regressions exploring the associations of ghrelin, adiponectin and leptin with age, gender, BMI z-score, HOMA-IR and tanner stage as independent variables showed strong associations of leptin levels with weight status and insulin resistance at baseline. The models accounted for 58 % of variability in leptin levels compared with 26 and 15 % for adiponectin and ghrelin levels respectively. In 83 children who completed the program, significant decreases in BMI z-score in overweight and obese children were noted. Leptin levels decreased significantly only in the obese group whereas adiponectin concentrations increased significantly in the three groups, In obese children, a significant correlation was found between changes in BMI Z-score, and changes in leptin levels (r = 0.39; P = 0.049) but not with changes in adiponectin levels. Conclusions: Abdominal obesity and insulin resistance were highly prevalent in obese children highlighting their risk of metabolic complications in adulthood. A 1-year long lifestyle intervention was associated with improvement in BMI z-score and metabolic parameters.
ISSN: 1743-7075

Droits : http://creativecommons.org/licenses/by/
hal-01231394
http://hal.upmc.fr/hal-01231394
http://hal.upmc.fr/hal-01231394/document
http://hal.upmc.fr/hal-01231394/file/art_10.1186_s12986-015-0039-9.pdf
DOI : 10.1186/s12986-015-0039-9