Éditeur(s) : Pêche thonière et dispositifs de concentration de poissons, Caribbean-Martinique, 15-19 Oct 1999
Résumé : In 1998, the French and Spanish tuna-boat owners associations have volentarily reconducted the Atlantic tuna protection plan (also called moratorium) initiated the previous year. This plan which has become an iccat recommendation for 1999, consists mainly in preventing fishing on floating objects (logs) and in having observers on board tuna purse seiners. Data collected by these observers and landing statistics allow to draw preliminary conclusions on some effects of the protection plan, on the fleets and on the Atlantic tuna stocks. Largely respected, the moratorium has led to a spatial redistribution of the fishing effort, an important reduction of the proportion of sets on logs and a decrease of the landings by one third compared to those realised the years before during the same months, particularily for the skipjack and the bigeye tunas. Moreover, data collected allow to calculate catch rates for a number of by-catch species in the purse seine fishery.

Droits : info:eu-repo/semantics/openAccess
http://archimer.ifremer.fr/doc/00042/15313/12660.pdf
http://archimer.ifremer.fr/doc/00042/15313/

Éditeur(s) : HAL CCSD Taylor & Francis (Routledge)
Résumé : International audience
It was in 1969, six years after the creation of the first sports fédération for the physically disabled in France, that regular ties with the Ministry of Youth and Sports began to develop. The aim of this article is to question how these ties affected the development of the movement towards the model of ‘classic’ sports federations. Between 1968 and 1973, data from the Ministry of Youth and Sports kept in the National Archives, along with information published in the federal press (crossed with accounts from persons with prominent roles d in disabled sports at the time), reveals a reorganization of the internal relations between managers (who are physically disabled people) and doctors (who generally come from the field of re-education). These new circumstances entailed their collaboration with another federal agent, who consequently became centrally important: the sports technician.
ISSN: 1743-0437

hal-01681359
https://hal.archives-ouvertes.fr/hal-01681359
DOI : 10.1080/17430437.2016.1273610

Éditeur(s) : Elsevier Sci Ltd
Résumé : Environmental management decisions based upon indicators are the end point of a process involving stakeholders and scientists. These steps should be explicit and follow a chronology. This paper presents a general framework for the design and use of management-oriented indicators, integrating management questions and performance criteria. We first examined the desirable characteristics of indicators aimed at providing decision-support for marine environmental management. Ideally, one should select the indicator that guarantees a safe and unambiguous decision leading to the appropriate measures in terms of regulation, remediation or control. In the present study, indicators are assessed according to two criteria: relevance and effectiveness. Relevance encompasses sensitivity and the existence of quantitative reference values, thereby allowing the selection of potential indicators. Effectiveness is the ability of the indicator to reach its predefined targets based on optimal (or at least improved) data collection protocols. The framework is illustrated by applying it to the European Water Framework Directive and to the Marine Protected Area management contexts. (C) 2010 Elsevier Ltd. All rights reserved.
Ocean & Coastal Management (0964-5691) (Elsevier Sci Ltd), 2011-01 , Vol. 54 , N. 1 , P. 84-92

Droits : 2010 Elsevier Ltd All rights reserved.
http://archimer.ifremer.fr/doc/00032/14331/11638.pdf
DOI:10.1016/j.ocecoaman.2010.10.037
http://archimer.ifremer.fr/doc/00032/14331/

Éditeur(s) : HAL CCSD
Résumé : International audience
Kisspeptins are well known as mediators of the coordinated communication between the brain-pituitary axis and the gonads in many vertebrates. To test the hypothesis that gonadal steroids regulate kiss1 and kiss2 mRNA expression in European sea bass (a teleost fish), we examined the brains of gonad-intact (control) and castrated animals, as well as castrated males (GDX) and ovariectomized females (OVX) that received testosterone (T) and estradiol (E2) replacement, respectively, during recrudescence. In GDX males, low expression of kiss1 mRNA is observed by in situ hybridization in the caudal hypothalamus (CH) and the mediobasal hypothalamus (MBH), although hypothalamic changes in kiss1 mRNA levels were not statistically different among the groups, as revealed by real-Time PCR. However, T strongly decreased kiss2 expression levels in the hypothalamus, which was documented in the MBH and the nucleus of the lateral recess (NRLd) in GDX T-Treated sea bass males. Conversely, it appears that E2 evokes lowkiss1mRNAin the CH, while there were cells expressing kiss2 in the MBH and NRLd in these OVX females. These results demonstrate that kisspeptin neurons are presumably sensitive to the feedback actions of sex steroids in the sea bass, suggesting that the MBH represents a major site for sex steroid actions on kisspeptins in this species. Also, recent data provide evidence that both positive and negative actions occur in key factors involved in sea bass reproductive function, including changes in the expression of gnrh-1/gonadotropin, cyp19b, er and ar genes and sex steroid and gonadotropin plasma levels in this teleost fish. © 2016. Published by The Company of Biologists Ltd.
Journal of Experimental Biology

hal-01406072
https://hal-univ-rennes1.archives-ouvertes.fr/hal-01406072
DOI : 10.1242/jeb.137364
PUBMED : 27591305

Éditeur(s) : Université des Antilles Études caribéennes
Résumé : The current consensus on Sustainable Tourism Development emphasizes the necessity for all tourist planning and policy making decisions to consider the impacts of tourism activity on all three dimensions of sustainability: economic efficiency referring to the capacity of an economy to be competitive, social justice aiming at the better distribution of produced welfare in the society and environmental preservation as the conservation of ecosystem’s capacity to provide humans with goods and services essential for their survival.A successful evaluation of the tourism activity presupposes the extensive examination of certain elements of the tourism product itself (expressed as characteristics of supply, demand and the organization of the tourist market), along a series of parameters related directly to the tourism products (effects), and indirectly to its implications for the destination (impacts). This paper presents a methodological framework for an integrated evaluation and monitoring of the tourism activity, in support of planners and policy makers towards both tourism and regional policy.A case study on the island of Samos in Greece is employed to demonstrate the scope of the methodology based on real data, its potential value but also its limits. The results of the study indicate that tourism remains the main developmental force in the island for over two decades without unfortunately, succeeding to deviate from the rule of decreasing tendencies, of similar mass tourism destination.
Le consensus actuel sur le développement du tourisme durable met l’emphase sur la nécessité pour tous les porteurs de projets et décideurs de prendre en compte les impacts de l’activité touristique sur les trois dimensions de la durabilité : l’efficience économique se réfère à la capacité d’une économie à être compétitive, la justice sociale aspire à une meilleure redistribution des richesses au sein de la société et la préservation de l’environnement concerne la préservation de la capacité des écosystèmes à fournir aux humains les biens et services essentiels à leur survie.Une évaluation réussie de l’activité touristique présuppose un examen exhaustif de certains éléments du produit touristique en lui-même (caractéristiques de l’offre, de la demande et de l’organisation du marché touristique), ainsi qu’une série de paramètres liée directement au produit touristique (effet). Il faut également prendre en compte les implications directes et indirectes du développement touristique de la destination (impacts). Cette recherche présentera un cadre méthodologique pour l’évaluation et la gestion intégrée de l’activité touristique, pour accompagner l’élaboration de politiques publiques et ce tant au niveau du tourisme que des politiques régionales.Une étude de cas réalisée sur l’île de Samos en Grèce sera utilisée pour démontrer la portée de la méthodologie utilisée (basée sur des résultats réels), sa valeur potentielle, ainsi que ses limites. Les résultats de l’étude indiquent que, depuis deux décennies, le tourisme est la principale force de développement de l’île sans, malheureusement, déroger à la règle des tendances à la baisse qui affecte également d’autres destinations touristiques similaires.
Grèce
Samos

Droits : info:eu-repo/semantics/openAccess
urn:doi:10.4000/etudescaribeennes.6257
http://journals.openedition.org/etudescaribeennes/6257

Résumé : This study aims to integrate the problems associated with port development and related activities in an economic monitoring programme that surveys all the activities associated with the marine environment. The economic monitoring programme in this sector also includes studying coastal spatial planning. This type of study concerns the creation and remodelling of sites that involve investments. We can consider two types of coastal spatial planning: one which integrates port development, tourism, aquaculture and fisheries and one that deals with the marine environment (Abric, 1989). Economic surveys of port development on French coasts supposes that the problem of identifying information sources have been resolved and the sectors and the activities that should be accounted for have been characterised. In what follows, the limits of available information and the problems pertaining to the proposed classification of activities according to statistical nomenclature will be discussed. The absence of structured and localised data is aggravated in certain cases by the rules of confidentiality. In light of these difficulties, the first part of this report is devoted to the inventory of general information sources and to the characterisation of existing data. As information forms the basis of any economic monitoring programme, it is necessary to know where, how and by whom information is processed and the limits that one comes up against. This report does not pretend to be exhaustive given the deadlines and the duration of this study. The second part of this report constitutes a summary of the recent trends discerned in terms of port development, from a legal, as well as economic and financial, point of view. This study should be considered as the first step to establishing a basis for a more in-depth study.
Cette étude a pour but d'intégrer les problèmes d'aménagement portuaire et les activités qui y sont liées dans un suivi conjoncturel de l'ensemble des activités économiques en relation avec le milieu maritime. Le suivi conjoncturel de ce secteur économique passe aussi par l'étude de l'aménagement physique du littoral. Celui-ci concerne la création et le remodelage de sites impliquant des investissements. On peut considérer deux types d'aménagements physiques -l'aménagement du littoral : portuaire, touristique, aquacole, halieutique; -l'aménagement du mineur marin (Abric, 1989). Un suivi conjoncturel de l'aménagement portuaire du littoral français, suppose en préalable la résolution du problème de l'identification des sources d'information et la caractérisation des secteurs et activités en prendre en compte. On verra par la suite les limites de l'information disponible et les problèmes afférents a la classification des activités selon les nomenclatures statistiques proposées. L'absence de données structurées et localisées est aggravée dans certains cas par des règles de confidentialité. Du fait de ces difficultés, la première partie de ce rapport est consacrée au recensement des sources d'information générale et à la caractérisation des données existantes. L'information étant à la base de tout suivi conjoncturel, il convient en effet d'être en mesure de savoir où, par qui et comment elle est traitée et les limites auxquelles on se heurte. Ce rapport ne peut prétendre à l'exhaustivité compte tenu des délais et de la période d'exécution de cette étude. La seconde partie constitue une première synthèse sur les évolutions récentes saisies en termes d'aménagements portuaires, tant d'un point de vue juridique qu'économique et financier. Cette étude est à considérer comme une première étape destinée à poser les bases pour une étude plus approfondie. [OCR NON CONTRÔLE]

Droits : info:eu-repo/semantics/openAccess
http://archimer.ifremer.fr/doc/1992/rapport-4169.pdf
http://archimer.ifremer.fr/doc/00000/4169/

Éditeur(s) : HAL CCSD Portland Press
Résumé : International audience
Lutheran (Lu) blood group and Basal Cell Adhesion Molecule (BCAM) antigens both reside on two glycoprotein (gp) isoforms, Lu and Lu(v13), that differ by the size of their cytoplasmic tail. They are receptors of laminin-10/11 and are expressed in red blood cells (RBCs), epithelial cells of multiple tissues and vascular endothelial cells. To gain more insights into the biological function of Lu/BCAM gps, we looked for potential partners of their cytoplasmic tail. We isolated Ubc9 (Ubiquitin-conjugating enzyme 9) protein by screening a human kidney library using the yeast two-hybrid system. Lu/Ubc9 interaction was validated by GST pull-down and co-immunoprecipitation experiments. Endogenous Ubc9 formed a complex with endogenous or recombinant Lu gp in A498 and MDCK epithelial cells, respectively. Substitution of lysine 585 by alanine in the Lu gp, abolished in vitro and ex vivo interaction of Lu gp with Ubc9 protein. Lu K585A mutant transfected in MDCK cells exhibited a normal basolateral membrane expression but was overexpressed at the surface of polarized MDCK cells as compared to wild-type Lu. Pulse-chase experiments showed extended half-life of Lu K585A gp at the plasma membrane, suggesting an impaired endocytosis of this mutant leading to protein accumulation at the membrane. Furthermore, we showed that the ability of MDCK-Lu K585A cells to spread on immobilized laminin was dramatically decreased. Our data supports a physiological role for the direct interaction between Lu gp and Ubc9 protein, and reveals a role for this enzyme in regulating the stability of Lu gp at the cell membrane.
ISSN: 0264-6021

hal-00478614
https://hal.archives-ouvertes.fr/hal-00478614
https://hal.archives-ouvertes.fr/hal-00478614/document
https://hal.archives-ouvertes.fr/hal-00478614/file/PEER_stage2_10.1042%252FBJ20060861.pdf
DOI : 10.1042/BJ20060861

Éditeur(s) : HAL CCSD
Résumé : International audience
Astrocytes send out long processes that are terminated by endfeet at the vascular surface and regulate vascular functions as well as homeostasis at the vascular interface. To date, the astroglial mechanisms underlying these functions have been poorly addressed. Here we demonstrate that a subset of messenger RNAs is distributed in astrocyte endfeet. We identified, among this transcriptome, a pool of messenger RNAs bound to ribosomes, the endfeetome, that primarily encodes for secreted and membrane proteins. We detected nascent protein synthesis in astrocyte endfeet. Finally, we determined the presence of smooth and rough endoplasmic reticulum and the Golgi apparatus in astrocyte perivascular processes and endfeet, suggesting for local maturation of membrane and secreted proteins. These results demonstrate for the first time that protein synthesis occurs in astrocyte perivascular distal processes that may sustain their structural and functional polarization at the vascular interface.
Cell Discovery

hal-01545319
https://hal.archives-ouvertes.fr/hal-01545319
DOI : 10.1038/celldisc.2017.5

Éditeur(s) : Academic Press Ltd- Elsevier Science Ltd
Résumé : The understanding of reciprocal interactions between Crassostrea gigas and Vibrio sp., whether these be virulent or avirulent, is vital for the development of methods to improve the health status of cultured oysters. We describe an original non-invasive experimental infection technique using cohabitation, designed to explore these interactions. Using real-time PCR techniques we examined the dynamics of virulent and avirulent Vibrio sp. in oyster hemolymph and tank seawater, and made a parallel study of the expression of four genes involved in oyster immune defense: Cg-BPI, Cg-EcSOD, Cg-IκB, Cg-TIMP. No mortality occurred in control animals, but oysters put in cohabitation for 2–48 h with animals previously infected by two Vibrio pathogens suffered mortalities from 2 to 16 days post-cohabitation. Our results show that virulent Vibrio infect healthy individuals after only 2 h of cohabitation, with values ranging from 4.5×102 to 2×104 cells ml−1 hemolymph. Simultaneously, an approximate ten-fold increase of the total Vibrio population was observed in control animals, with a 6.6–78.5-fold up-expression of targeted genes. In contrast, oysters exposed to harmful bacteria had mean expression levels strongly down-regulated by a factor of 9.2–29 (depending on the gene) compared with control animals. Although oysters were still found to be infected by virulent Vibrio after 6–48 h of cohabitation, no significant differences were noted when comparing levels of each transcript in control and infected oysters at the same sampling times during this period: the important differences were noted before 6 h cohabitation. Taken together, our data support (1) the hypothesis that virulent Vibrio disturbs the immune response of this invertebrate host both rapidly and significantly, although this occurs specifically during an early and transient period during the first 6 h of cohabitation challenge, and that (2) expression of targeted genes is not correlated with vibriosis resistance.
Fish & Shellfish Immunology (1050-4648) (Academic Press Ltd- Elsevier Science Ltd), 2011-02 , Vol. 30 , N. 2 , P. 691-699

Droits : 2011 Elsevier Ltd All rights reserved.
http://archimer.ifremer.fr/doc/00025/13604/10681.pdf
DOI:10.1016/j.fsi.2010.12.017
http://archimer.ifremer.fr/doc/00025/13604/

Éditeur(s) : HAL CCSD Humana Press
Résumé : International audience
The sodium-proton exchanger 1 (NHE-1) is a membrane transporter that exchanges Na(+) for H(+) ion across the membrane of eukaryotic cells. It is cooperatively activated by intracellular protons, and this allosteric regulation is modulated by the biophysical properties of the plasma membrane and related lipid environment. Consequently, NHE-1 is a mechanosensitive transporter that responds to osmotic pressure, and changes in membrane composition. The purpose of this study was to develop the relationship between membrane surface tension, and the allosteric balance of a mechanosensitive transporter such as NHE-1. In eukaryotes, the asymmetric composition of membrane leaflets results in a difference in surface tensions that is involved in the creation of a reservoir of intracellular vesicles and membrane buds contributing to buffer mechanical constraints. Therefore, we took this phenomenon into account in this study and developed a set of relations between the mean surface tension, membrane asymmetry, fluid phase endocytosis and the allosteric equilibrium constant of the transporter. We then used the experimental data published on the effects of osmotic pressure and membrane modification on the NHE-1 allosteric constant to fit these equations. We show here that NHE-1 mechanosensitivity is more based on its high sensitivity towards the asymmetry between the bilayer leaflets compared to mean global membrane tension. This compliance to membrane asymmetry is physiologically relevant as with their slower transport rates than ion channels, transporters cannot respond as high pressure-high conductance fast-gating emergency valves.
ISSN: 1085-9195

inserm-00871709
http://www.hal.inserm.fr/inserm-00871709
http://www.hal.inserm.fr/inserm-00871709/document
http://www.hal.inserm.fr/inserm-00871709/file/art_3A10.1007_2Fs12013-012-9340-7.pdf
DOI : 10.1007/s12013-012-9340-7

Éditeur(s) : HAL CCSD Elsevier
Résumé : International audience
The correlation between the flow turbulence and the performances of a ma- rine current turbine is studied. First, the incoming flow encountered in the flume tank is characterized in the framework of fully developed turbulent cascades in the inertial range. The Reynolds number, the Kolmogorov dissipation scale and the integral scale, are estimated from flow measurements. The intermittency of the turbulence is characterized in the lognormal multifractal framework, and the influence of the turbulent flow on the turbine power is assessed. The rotor speed control unit characteristics used for the turbine regulation induces non-negligible effects on the turbine behavior under fluctuations loads. Even if the power spectrum does not reveal any scale invariance, a multiscale analysis allows us to show the correlations between the turbulence time se- ries and the power produced. The classical Mean Square Coherency function shows that for scales larger than 10 seconds, the upstream velocity and power have large correlations. In the framework of the Empirical Mode Decomposition method, such correlations are studied using the time-dependence intrinsic corre- lation analysis method. This method allows to zoom into time-frequency scales where the flow perturbations induced some modifications in power production
ISSN: 0960-1481

hal-01528296
https://hal.archives-ouvertes.fr/hal-01528296
DOI : 10.1016/j.renene.2017.05.024

Éditeur(s) : ICES 2008 Annual Science Conference, 22-26 september 2008, HALIFAX, CANADA
Résumé : While the majority of small-scale coastal fisheries are found in developing nations, a considerable number exist in developed nations as well. Indeed, small-scale fisheries are strongly represented in all European Union (EU) Member States, 81% and 87% of the EU 25 whole fleet is composed of vessels less than 12 and 15 meters long respectively. In 2000, European regulation established a fisheries data collection framework including economic information requirements in order to provide the scientific basis for the implementation of the Common Fisheries Policy. This paper presents the methodology designed to collect the relevant economic data and characterize the status of the French fleets from an economic perspective. Based on stratified sampling plan including the following of small-scale fleets, data collection by field survey is based on a single questionnaire for all the fleets, whatever the size of the vessel, the gear used, the fishing area or the species targeted. It provides a large data set including earnings and costs but also capital, employment, fishing activity figures. Statistical analysis through a revenue model gives the basis for a re-assessing of the small-scale fisheries contribution to national production. Economic indicators such as gross added value, revenues, and direct employment are derived from the data set. The significant role of small scale fisheries in the French fishing sector is established and the impact of the rising fuel costs in fisheries is discussed.

Droits : 2008 ICES
http://archimer.ifremer.fr/doc/00059/16998/14500.pdf
http://archimer.ifremer.fr/doc/00059/16998/

Éditeur(s) : HAL CCSD Elsevier
Résumé : International audience
Purpose The purpose of this review was to examine the comprehensively accumulated data regarding potential therapeutic aspects of exogenous administration of interleukin 33 (IL-33) or its antagonists in allergic, cancerous, infectious, and inflammatory diseases. Methods A selected review was undertaken of publications that examined the protective and exacerbating effects of IL-33 or its inhibitors in different diseases. Mechanisms of action are summarized to examine the putative role of IL-33 in various diseases. Findings IL-33 promoted antibacterial, antiviral, anti-inflammatory, and vaccine adjuvant functions. However, in TH2-biased respiratory, allergic, parasitic, and inflammatory conditions, IL-33 exhibited disease-sensitizing effects. The alarmin cytokine IL-33 induced protective effects in diseases via recruitment of regulatory T cells; antiviral CD8+ cells, natural killer cells, γδ T cells, and nuocytes; antibacterial and antifungal neutrophils or macrophages; vaccine-associated B/T cells; and inhibition of nuclear factor–κB–mediated gene transcription. In contrast, IL-33 exacerbated the disease process by increasing TH2 cytokines, IgE and eosinophilic immune responses, and inhibition of leukocyte recruitment in various diseases. Implications The protective or exacerbated aspects of use of IL-33 or its inhibitors are dependent on the type of infection or inflammatory condition, duration of disease (acute or chronic), organ involved, cytokine microenvironment, dose or kinetics of IL-33, and genetic predisposition. The alarmin cytokine IL-33 acts at cellular, molecular, and transcriptional levels to mediate pluripotent functions in various diseases and has potential therapeutic value to mitigate the disease process
ISSN: 0149-2918

hal-01295637
https://hal-univ-rennes1.archives-ouvertes.fr/hal-01295637
PUBMED : 26992663
DOI : 10.1016/j.clinthera.2016.02.021

Éditeur(s) : HAL CCSD
Résumé : International audience
This study aims at understanding the presence of regulated and emerging micropollutants, particularly pesticides and pharmaceuticals, in surface water, regarding spatial and temporal influences at a watershed scale. The study of relations between micropollutants and other water quality and hydroclimatic parameters was carried out from a statistical analysis on historical and experimental data of different sampling sites from the main watershed of Brittany, western France. The outcomes point out the influence of urban and rural areas of the watershed as well as the impact of seasons on contamination variations. This work contributes to health risk assessment related to surface water contamination by micropollutants. This approach is particularly interesting in the case of agricultural watersheds such as the one studied, where more than 80% of surface water is used to produce drinking water.
International Journal of Environmental Research and Public Health

hal-00878078
https://hal.archives-ouvertes.fr/hal-00878078
https://hal.archives-ouvertes.fr/hal-00878078/document
DOI : 10.3390/ijerph9124433

Éditeur(s) : HAL CCSD
Résumé : International audience
AIM:We aimed to study the relationships between circulating 25-hydroxyvitamin D [25(OH)D], insulin resistance and leptin-to-adiponectin (L/A) ratio in Guadeloupean children and adolescents and to analyse the changes in 25(OH)D levels after a 1-year lifestyle intervention program.METHODS:25(OH)D concentrations were measured via a chemiluminescence assay. Cardiometabolic risk factors, homoeostasis model assessment of insulin resistance (HOMA-IR), and adipokines were measured. The lifestyle intervention included dietary counselling, regular physical activity.RESULTS:Among 117 girls and boys (11–15 years old, 31.6% obese), 40% had vitamin D deficiency (25(OH)D levels < 20 ng/mL). With linear regression models where 25(OH)D and HOMA-IR acted as independent variables and age, sex, BMI, L/A ratio as covariates, 25(OH)D was significantly associated with HOMA-IR alone (P = 0.036). HOMA-IR was also associated with BMI z-score ≥ 2, L/A ratio and an interaction term BMI z-score ≥ 2*L/A ratio (P < 0.001 for all). After one year, in 78 children/adolescent, mean serum 25(OH)D increased significantly from 21.4 ± 4.9 ng/mL at baseline to 23.2 ± 6.0 after 1 year; P = 0.003 whereas BMI z-score, HOMA-IR and L/A ratio decreased significantly (P = 0.003, P < 0.001 and P = 0.012; respectively).CONCLUSION:The association between 25(OH)D and HOMA-IR, independently of obesity and the high prevalence of vitamin D deficiency should be considered in order to prevent the later incidence of T2DM. A healthy lifestyle including non-sedentary and outdoor activities could be a way for improving vitamin D status.
Open Access Macedonian Journal of Medical Sciences

hal-01427889
http://hal.upmc.fr/hal-01427889
PUBMEDCENTRAL : PMC5175505

Éditeur(s) : HAL CCSD Elsevier
Résumé : International audience
The last step of oestrogen biosynthesis is catalyzed by the enzyme aromatase, the product of the cyp19a1 gene. In vertebrates, cyp19a1 is expressed in the brain resulting in a local oestrogen production that seems important not only for the control of reproduction-related circuits and sexual behaviour, but also for the regulation of neural development, synaptic plasticity and cell survival. In adult amphibians, the precise sites of expression of cyp19a1 in the brain have not been investigated which prevents proper understanding of its potential physiological functions. The present study aimed at examining the precise neuroanatomical distribution of cyp19a1 transcripts in adult brains of both male and female Xenopus. We found that cyp19a1 expression is highly regionalized in the brains of both sexes. The highest expression was found in the anterior part of the preoptic area and in the caudal hypothalamus, but significant levels of cyp19a1 transcripts were also found in the supraoptic paraventricular and suprachiasmatic areas, and in brain regions corresponding to the septum, bed nucleus of the stria terminalis and amygdala. Importantly, no obvious difference between male and female Xenopus was detected at the level of cyp19a1 transcripts. Additionally, in the brain of adult Xenopus, cyp19a1 transcripts were detected in neurons, and not in glial cells. These data and those available in other vertebrates on cyp19a1/aromatase expression suggest that, with the intriguing exception of teleost fishes, cyp19a1 was under strong evolutionary conservation with respect to its sites of expression and the nature of the cells in which it is expressed
ISSN: 0016-6480

hal-01187140
https://hal-univ-rennes1.archives-ouvertes.fr/hal-01187140
DOI : 10.1016/j.ygcen.2015.08.008
PUBMED : 26255686

Éditeur(s) : HAL CCSD American Association for the Advancement of Science
Résumé : International audience
DNA double-strand breaks (DSBs) are introduced in meiosis to initiate recombination and generate crossovers, the reciprocal exchanges of genetic material between parental chromosomes. Here, we present high-resolution maps of meiotic DSBs in individual human genomes. Comparing DSB maps between individuals shows that along with DNA binding by PRDM9, additional factors may dictate the efficiency of DSB formation. We find evidence for both GC-biased gene conversion and mutagenesis around meiotic DSB hotspots, while frequent colocalization of DSB hotspots with chromosome rearrangement breakpoints implicates the aberrant repair of meiotic DSBs in genomic disorders. Furthermore, our data indicate that DSB frequency is a major determinant of crossover rate. These maps provide new insights into the regulation of meiotic recombination and the impact of meiotic recombination on genome function.
ISSN: 0036-8075

hal-01123717
https://hal-univ-rennes1.archives-ouvertes.fr/hal-01123717
PUBMED : 25395542
DOI : 10.1126/science.1256442

Éditeur(s) : HAL CCSD Nature Publishing Group
Résumé : The Amazon basin harbors a diverse ecological community that has a critical role in the maintenance of the biosphere. Although plant and animal communities have received much attention, basic information is lacking for fungal or prokaryotic communities. This is despite the fact that recent ecological studies have suggested a prominent role for interactions with soil fungi in structuring the diversity and abundance of tropical rainforest trees. In this study, we characterize soil fungal communities across three major tropical forest types in the western Amazon basin (terra firme, seasonally flooded and white sand) using 454 pyrosequencing. Using these data, we examine the relationship between fungal diversity and tree species richness, and between fungal community composition and tree species composition, soil environment and spatial proximity. We find that the fungal community in these ecosystems is diverse, with high degrees of spatial variability related to forest type. We also find strong correlations between alpha- and beta-diversity of soil fungi and trees. Both fungal and plant community beta-diversity were also correlated with differences in environmental conditions. The correlation between plant and fungal richness was stronger in fungal lineages known for biotrophic strategies (for example, pathogens, mycorrhizas) compared with a lineage known primarily for saprotrophy (yeasts), suggesting that this coupling is, at least in part, due to direct plant-fungal interactions. These data provide a much-needed look at an understudied dimension of the biota in an important ecosystem and supports the hypothesis that fungal communities are involved in the regulation of tropical tree diversity.
ISSN: 1751-7362

hal-01032405
https://hal.archives-ouvertes.fr/hal-01032405
DOI : 10.1038/ismej.2013.66

Éditeur(s) : HAL CCSD Wiley-Blackwell
Résumé : International audience
The spatio‐temporal dynamics of disease spread provide an area of acute scientific interest but substantial analytic challenges. Analytic approaches need to draw from current knowledge of disease systems yet be flexible enough to provide targeted inference without restrictive assumptions. We explore this balance in detail with respect to the observed pattern of spread of sugarcane yellow leaf virus, the causal agent of yellow leaf disease. This disease affects cultivated sugarcane plants and spreads via an aphid vector (Melanaphis sacchari). The system is of considerable economic interest, but presents several analytic complications. First, the plants begin as well separated individuals that grow into large plants with overlapping leaf canopies enabling increased vector access between neighbouring plants. The overlap between neighbouring plants first occurs within rows and then extends between rows, presenting another interesting spatial component to consider when modelling the propagation of infection. Second, the vector initially flies between plants but at some point sheds wings and shifts to walking between plants. We are particularly interested in how these two features of the system and their interrelation may impact the spatial spread of disease over time. In addition, we also explore the impact of potential immigration of infected vectors from outside of the study area. We treat our observed cane field as a spatial grid with regular spacing between rows and smaller spacing within rows. Our data consist of observed disease status of each of 1643 plants at each of six time points. We provide descriptive summaries of the temporal evolution of spatial pattern in infection, then propose hierarchical models to describe the pattern and capture the influence of the ecological and environmental factors listed above.
ISSN: 0143-9782

hal-00684235
https://hal.archives-ouvertes.fr/hal-00684235

Éditeur(s) : HAL CCSD IWA Publishing
Résumé : International audience
The widely occurring degradation product aminomethylphosphonic acid (AMPA) is a result of glyphosate and amino-polyphosphonate degradation. Massive use of the parent compounds leads to the ubiquity of AMPA in the environment, and particularly in water. The purpose of this review is to summarize and discuss current insights into AMPA formation, transport, persistence and toxicity. In agricultural soils, AMPA is concentrated in the topsoil, and degrades slowly in most soils. It can reach shallow groundwater, but rarely managed to enter deep groundwater. AMPA is strongly adsorbed to soil particles and moves with the particles towards the stream in rainfall runoff. In urban areas, AMPA comes from phosphonates and glyphosate in wastewater. It is commonly found at the outlets of Wastewater Treatment Plants (WWTP). Sediments tend to accumulate AMPA, where it may be biodegraded. Airborne AMPA is not negligible, but does wash-out with heavy rainfall. AMPA is reported to be persistent and can be biologically degraded in soils and sediments. Limited photodegradation in waters exists. AMPA mainly has its sources in agricultural leachates, and urban wastewater effluents. The domestic contribution to urban loads is negligible. There is a critical lack of epidemiological data - especially on water exposure - to understand the toxicological effects, if any, of AMPA on humans. Fortunately, well operated water treatment plants remove a significant proportion of the AMPA from water, even though there are not sufficient regulatory limits for metabolites.
ISSN: 0043-1354

hal-01516026
https://hal-univ-rennes1.archives-ouvertes.fr/hal-01516026
https://hal-univ-rennes1.archives-ouvertes.fr/hal-01516026/document
https://hal-univ-rennes1.archives-ouvertes.fr/hal-01516026/file/Grandcoin%20et%20al.%20-%20AminoMethylPhosphonic%20acid%20%28AMPA%29%20in%20natural%20water.pdf
DOI : 10.1016/j.watres.2017.03.055