Éditeur(s) : HAL CCSD Public Library of Science
Résumé : International audience
We conducted a survey along three belt transects located at increasing distances from the coast to determine whether a non-random arboreal ant assemblage, such as an ant mosaic, exists in the rainforest on the Masoala Peninsula, Madagascar. In most tropical rainforests, very populous colonies of territorially dominant arboreal ant species defend absolute territories distributed in a mosaic pattern. Among the 29 ant species recorded, only nine had colonies large enough to be considered potentially territorially dominant; the remaining species had smaller colonies and were considered non-dominant. Nevertheless, the null-model analyses used to examine the spatial structure of their assemblages did not reveal the existence of an ant mosaic. Inland, up to 44% of the trees were devoid of dominant arboreal ants, something not reported in other studies. While two Crematogaster species were not associated with one another, Brachymyrmex cordemoyi was positively associated with Technomyrmex albipes, which is considered an invasive species-a non-indigenous species that has an adverse ecological effect on the habitats it invades. The latter two species and Crematogaster ranavalonae were mutually exclusive. On the other hand, all of the trees in the coastal transect and at least 4 km of coast were occupied by T. albipes, and were interconnected by columns of workers. Technomyrmex albipes workers collected from different trees did not attack each other during confrontation tests, indicating that this species has formed a supercolony along the coast. Yet interspecific aggressiveness did occur between T. albipes and Crematogaster ranavalonae, a native species which is likely territorially dominant based on our intraspecific confrontation tests. These results suggest that the Masoala rainforest is threatened by a potential invasion by T. albipes, and that the penetration of this species further inland might be facilitated by the low density of native, territorially dominant arboreal ants normally able to limit its progression.
ISSN: 1932-6203

hal-00817444
https://hal.archives-ouvertes.fr/hal-00817444
https://hal.archives-ouvertes.fr/hal-00817444/document
https://hal.archives-ouvertes.fr/hal-00817444/file/Dejean2010_Spatial.pdf
DOI : 10.1371/journal.pone.0009319

Éditeur(s) : HAL CCSD Elsevier Masson
Résumé : International audience
The maintenance in the long run of a positive carbon balance under very low irradiance is a prerequisite for survival of tree seedlings below the canopy or in small gaps in a tropical rainforest. To provide a quantitative basis for this assumption, experiments were carried out to determine whether construction cost (CC) and payback time for leaves and support structures, as well as leaf life span (i) differ among species and (ii) display an irradiance-elicited plasticity. Experiments were also conducted to determine whether leaf life span correlates to CC and payback time and is close to the optimal longevity derived from an optimization model. Saplings from 13 tropical tree species were grown under three levels of irradiance. Specific-CC was computed, as well as CC scaled to leaf area at the metamer level. Photosynthesis was recorded over the leaf life span. Payback time was derived from CC and a simple photosynthesis model. Specific-CC displayed only little interspecific variability and irradiance-elicited plasticity, in contrast to CC scaled to leaf area. Leaf life span ranged from 4 months to >26 months among species, and was longest in seedlings grown under lowest irradiance. It was always much longer than payback time, even under the lowest irradiance. Leaves were shed when their photosynthesis had reached very low values, in contrast to what was predicted by an optimality model. The species ranking for the different traits was stable across irradiance treatments. The two pioneer species always displayed the smallest CC, leaf life span, and payback time. All species displayed a similar large irradiance-elicited plasticity.
ISSN: 1631-0691

hal-01095975
https://hal-agroparistech.archives-ouvertes.fr/hal-01095975
DOI : 10.1016/j.crvi.2011.01.007

Éditeur(s) : HAL CCSD Elsevier
Résumé : Over-exploitation and fragmentation are serious problems for tropical forests. Most sustainable forest management practices avoid clear-cuts and apply selective logging systems focused on a few commercial species. We applied a simulation model to estimate the impact of such selective logging scenarios on the genetic diversity and demography of four tropical tree species from French Guiana. The simulations used data on genetic and demographic composition, growth, phenology and pollen and seed dispersal obtained for Dicorynia guianensis, Sextonia rubra, Symphonia globulifera and Vouacapoua americana at the experimental site in Paracou. Whereas Symphonia globulifera serves as a model for a species with low logging pressure, the other three species represent the most exploited tree species in French Guiana. In simulations with moderate logging, typical for French Guiana, with large cutting diameter (>60 cm diameter) and long cutting cycles (65 years), the two species V. americana and Sextonia rubra were not able to recover their initial stock at the end of the rotation period, with a large decrease in the number of individuals and in basal area. Under a more intensive logging system (cutting diameter >45 cm diameter, cutting cycles of 30 years) that is common practice in the Brazilian Amazon, only Symphonia globulifera showed no negative impact. Generally, the differences between the genetic parameters in the control scenarios without logging and the logging scenarios were surprisingly small. The main reasons for this were the overlapping of generations and the effective dispersal ability of gene vectors in all species, which guarantee relative homogeneity of the genetic structure in different age classes. Nevertheless, decreasing the population size by logging reduced the number of genotypes and caused higher genetic distances between the original population and the population at the end of the logging cycles. Sensitivity analysis showed that genetic changes in the logging scenarios were principally determined by the growth, densities and cutting diameter of each species, and only to a very small extent by the reproductive system including factors such as pollen and seed dispersal and flowering phenology
ISSN: 0006-3207

hal-01032050
https://hal.archives-ouvertes.fr/hal-01032050
DOI : 10.1016/j.biocon.2006.02.014

Éditeur(s) : HAL CCSD Seoul National University
Résumé : http://www.episodes.org/backissues/302/abstract2.htm
International audience
The Trans-North China Belt (TNCB) is a Paleoproterozoic collisional orogen (ca. 1.9-1.8 Ga) responsible for the amalgamation of the North China Craton. Detail field works in Lüliangshan, Hengshan, Wutaishan and Fuping massifs where the belt is well exposed, allow us to draw new tectonic map and crustal-scale cross sections. The available petrologic, radiometric, geochronologic data are integrated in a geodynamic evolution scheme for this orogen. The Low Grade Mafic Unit (LGMU) is interpreted as an ophiolitic nappe rooted in a suture zone located in the western part of the Lüliangshan. This ophiolitic nappe overthrusts to the SE upon the Orthogneiss-Volcanites Unit (OVU) that consists of a bimodal volcanicsedimentary series metamorphosed under amphibolite facies conditions intruded by calcalkaline orthogneiss. The OVU is a composite Neoarchean-Paleoproterozoic magmatic arc developed during two stages (ca. 2500 and 2100 Ma) upon a continental basement corresponding to the western extension of the Neoarchean Fuping massif. The OVU overthrusts to the SE the Fuping massif along the Longquanguan shear zone. This stack of nappe, coeval with an amphibolite facies metamorphism, is dated at ca 1880 Ma. Subsequently, the metamorphic series experienced a widespread migmatization at 1850 Ma and was intruded by post-orogenic plutons dated at 1800 Ma. The weakly to unmetamorphosed Hutuo Supergroup unconformably overlies the metamorphosed and ductilely deformed units (OVU and LGMU), but it is also involved in a second tectonic phase developed in subsurface conditions. These structural features lead us to question the ca 2090 Ma age attributed to the Hutuo supergroup. Moreover, in the Fuping massif, several structural and magmatic lines of evidence argue for an earlier orogenic event at ca 2100 Ma that we relate to an older west-directed subduction below the Fuping Block. The Taihangshan Fault might be the location of a possible suture zone between the Fuping Block and an eastern one. A geodynamic model, at variance with previous ones, is proposed to account for the formation of the TNCB. In this scheme, three Archean continents, namely from West to East, the Ordos, Fuping and Eastern Blocks are separated by the Lüliang and Taihang Oceans. The closure of the Taihang Ocean at ca 2100 Ma by westward subduction below the Fuping Block accounts for the arc magmatism and the 2100 Ma orogeny. The second collision at 1900-1880 Ma between the Fuping and Ordos blocks is responsible for the main structural, metamorphic and magmatic features of the Trans-North China Belt.
ISSN: 0705-3797

insu-00152972
https://hal-insu.archives-ouvertes.fr/insu-00152972
https://hal-insu.archives-ouvertes.fr/insu-00152972/document
https://hal-insu.archives-ouvertes.fr/insu-00152972/file/Episodes_TNCB_Faure_et_al.pdf

Éditeur(s) : HAL CCSD
Résumé : Karst systems are the most important groundwater reservoirs in Mediterranean areas. Their structure and functioning are complex and not well known because of the high heterogeneity created and organised by groundwater flow. Water storage entities and water storage changes within remain difficult to assess by direct measurement and modelling. Geodetic monitoring is here used to constrain and bring original insights on karst system functioning. The Durzon karst system (Larzac plateau, Southern France) is monitored by several geodetic techniques: - Monthly absolute gravity measurements at three sites - Time-lapse surface to depth gravity measurements - Time-lapse microgravity surveying - Continuous long base hydrostatic tiltmeter monitoring at two sites Absolute gravity monitoring complemented with relative gravity surveys allows quantification of water storage changes on the studied karst system. Interannual water storage changes as detected by gravity are ~400 mm in amplitude. These storage changes are shown to occur in the epikarst and infiltration zone in certain areas. Water storage variations spatial heterogeneity is also demonstrated on the karst system. Tilt measurements monitor fast and slow infiltration processes within the karst fractured media. Karst system geodetic monitoring brings both quantitative and integrated hydrologic knowledge. Such methods could be applied to other karst aquifer studies.
Les aquifères karstiques constituent l'essentiel des ressources en eau souterraine sur le pourtour de la Méditerranée. Leur structure et leur fonctionnement sont complexes et mal connus à cause du processus de karstification qui hiérarchise la circulation hydrodynamique en leur sein. La localisation du stockage de l'eau et de ses variations au sein de tels aquifères est difficile à mesurer et à modéliser. Nous utilisons les outils de la géodésie pour apporter de nouvelles contraintes sur le fonctionnement hydrologique et les variations de stock d'eau en milieu karstique. Ainsi, le système karstique du Durzon, (Causse du Larzac, France) fait l'objet d'un suivi par plusieurs méthodes géodésiques incluant: - Des mesures de gravimétrie absolue en trois sites répétées de manière mensuelle ; - Des mesures répétées de gravimétrie relative entre la surface et la profondeur du karst ; - Des mesures répétées de microgravimétrie ; - Des mesures inclinométriques longue base en deux sites. Nous démontrons que le couplage entre la gravimétrie absolue et relative permet d'appréhender les variations de stock d'eau sur le bassin d'alimentation étudié. Les variations inter-anuelles observées sont de l'ordre de 400 mm, et nous montrons que celles-ci s'opèrent dans certaines zones au sein des horizons supérieurs du karst : épikarst et zone d'infiltration. Une forte hétérogénéité spatiale des variations de stockage est aussi mise en évidence à l'échelle du bassin d'alimentation. Les mesures inclinométriques permettent de suivre les phénomènes d'infiltration rapide et lente dans la masse karstique fracturée. Le suivi géodésique de ce système karstique permet d'apprécier quantitativement et de façon intégrée son fonctionnement hydrologique et pourra être transposé pour l'étude d'autres aquifère karstiques.
https://tel.archives-ouvertes.fr/tel-00404851

tel-00404851
https://tel.archives-ouvertes.fr/tel-00404851
https://tel.archives-ouvertes.fr/tel-00404851/document
https://tel.archives-ouvertes.fr/tel-00404851/file/THESE_jacob.pdf

Éditeur(s) : HAL CCSD Wiley-VCH Verlag
Résumé : International audience
Proteomic profiling plays a decisive role in the elucidation of molecular signatures representative of a specific clinical context. MuStem cell-based therapy represents a promising approach for clinical applications to cure Duchenne Muscular Dystrophy (DMD). To expand our previous studies collected in the clinically relevant DMD animal model, we decided to investigate the skeletal muscle proteome four months after systemic delivery of allogenic MuStem cells. Quantitative proteomics with isotope-coded protein labelling (ICPL) was used to compile quantitative changes in the protein expression profiles of muscle in transplanted Golden Retriever Muscular Dystrophy (GRMD) dogs as compared to GRMD dogs. A total of 492 proteins were quantified, including 25 that were overrepresented and 46 that were underrepresented after MuStem cell transplantation. Interestingly, this study demonstrates that somatic stem cell therapy impacts on the structural integrity of the muscle fascicle by acting on fibres and its connections with the extracellular matrix. We also show that cell infusion promotes protective mechanisms against oxidative stress and favours the initial phase of muscle repair. This study allows us to identify putative candidates for tissue markers that might be of great value in objectively exploring the clinical benefits resulting from our cell-based therapy for DMD.
ISSN: 1615-9853

Droits : info:eu-repo/semantics/OpenAccess
hal-01332030
https://hal.archives-ouvertes.fr/hal-01332030
https://hal.archives-ouvertes.fr/hal-01332030/document
https://hal.archives-ouvertes.fr/hal-01332030/file/Quantitative%20proteome%20profiling%20of%20dystrophic%20dog%20skeletal%20muscle_accepted.pdf
DOI : 10.1002/pmic.201600002

Éditeur(s) : HAL CCSD Sidestone Press
Résumé : International audience
In 2006, an INRAP team led by T. Romon proceeded to search the Amerindian dump of the Gare Maritime in Basse-Terre. An important collection of ceramics there was discovered and then examined by Mr. Hildebrand. The results made possible to assign the occupation to subseries of old huecan and Cedrosan Saladoid.As part of a master’s degree research in archaeology, a study of sets of this pottery was considered a new approach. The latter, inspired by an analysis ofJ. Van Berg on the Rubanné ceramics, can be called structural. Indeed, the search for elementary motifs in this type of decoration, said abstract, can reconstruct it by studying how the motifs fit together. We can compare the motifs to a panel of vocabulary, which combine according to rules of "grammar" defined.This method thus relies on a precise statistical analysis that highlights thecombinatorial rules by inserting an additional factor: that of the various decorating techniques (etching, engraving, painting, modeling).These rules can be translated simply by notions of "presence / absence" or by incorporating a notion of frequency depending on the level of complexity of the decoration. This is the number of motifs creating the set. We call this the set of one or two motifs for simple associations or of four or five motifs for complex sets.The objective of this study is to express an early hypothesis, which would tend to reveal the existence or not of a specific hierarchy of elementary motifs depending on various factors such as complexity of the set or the technique used in both huecan and Cedrosan subseries.
En 2006, un equipo del INRAP dirigido por T. Romon procedió a la excavación del vertedero amerindio de la Estación Marítima en Bsse-terre. Una importante colección de cerámica fue puesta allí al día y a continuación estudiada por el Sr. Hildebrand. Los resultados obtenidos permitieron de asignar esta ocupación a las subseries del Saladoïde huecan y cedrosan antiguos.En el marco de un curso de posgrado en arqueologia, se previo un estudio de los decorados de este conjunto de cerámica según un nuevo enfoque. Esta última, inspirada en un análisis realizado por J. Van Berg, en la cerámica Rubannée,se puede llamar estructural. En efecto, la búsqueda de los motivos elementales en este tipo de decorado, dicho abstracto, permiten reconstruirlo estudiando la forma en que los motivos se arreglan. Podemos comparar los motivos a un cuadro de vocabulario que se combina según normas “gramaticales” definidas.Este método se basa, pues en un análisis de estadística precisa que pone de relieve las reglas combinatorias mediante la inserción de un factor adicional: la delas diferentes técnicas de decoración (aguafuerte, grabado, pintura, modelado).Estas normas pueden traducirse simplemente en conceptos de “presencia/ausencia” o incorporando un concepto de frecuencia según el nivel de complejidad del decorado. Este último corresponde entre motivos que crean el decorado. Hablamos entonces de decorado a unos o dos motivos para las asociaciones simples o a cuatro o cinco motivos para los decorados complejos.El objetivo de este estudio es de emitir un principio de hipótesis que tendería a revelar la existencia, o no, de una jerarquía específica de los motivos elementales en función de los distintos factores, como la complejidad del decorado o la técnica utilizada, en las dos subseries huecan y cedrosan
En 2006, une équipe de l'INRAP dirigée par T. Romon procéda à la fouille du dépotoir amérindien de la Gare Maritime à Basse-Terre. Une importante collection de céramique y fut mise au jour et ensuite étudiée par M. Hildebrand. Les résultats obtenus permirent d'attribuer cette occupation aux sous-séries du Saladoïde huecan et cedrosan ancien. Dans le cadre d'un master en archéologie, une étude des décors de cet ensemble céramique fut envisagée selon une nouvelle approche. Cette dernière, inspirée d'une analyse effectuée par J. Van Berg sur la céramique du Rubané, peut être qualifiée de structurale. En effet, la recherche des motifs élémentaires dans ce type de décor, dit abstrait, permet de le reconstruire en étudiant la façon dont les motifs s'agencent. Nous pouvons comparer les motifs à un panel de vocabulaire qui se combine selon des règles « grammaticales » définies.Cette méthode s'appuie ainsi sur une analyse statistique précise qui met en exergue les règles combinatoires en insérant un facteur supplémentaire : celui des différentes techniques de décoration (gravure, incision, peinture, modelage). Ces règles peuvent se traduire simplement par des notions de « présence / absence » ou bien en incorporant une notion de fréquence selon le niveau de complexité du décor. Ce dernier correspond au nombre de motifs créant le décor. Nous parlons alors de décor à un ou deux motifs pour les associations simples ou bien à quatre ou cinq motifs pour les décors complexes. L'objectif de cette étude est d'émettre un début d’hypothèse qui tendrait à révéler l'existence, ou non, d'une hiérarchie spécifique des motifs élémentaires en fonction des différents facteurs, comme la complexité du décor ou la technique utilisée, chez les deux sous-séries huecanes et cedrosanes.
Archéologie Caraïbe

Droits : info:eu-repo/semantics/OpenAccess
ISBN : 978-90-8890-291-8
halshs-01140826
https://halshs.archives-ouvertes.fr/halshs-01140826
https://halshs.archives-ouvertes.fr/halshs-01140826/document
https://halshs.archives-ouvertes.fr/halshs-01140826/file/Honore%20%26%20Berard%202014%20-%20Etude%20analytique%20des%20decors%20ceramiques%20du%20site%20de%20la%20Gare%20Maritime.pdf

Éditeur(s) : Elsevier Science Bv
Résumé : Estuaries and coastal waters are essential nursery habitats for many marine species, and especially for flatfishes. Thus, investigating how anthropogenic disturbances affect the quality of these habitats is of major importance to understand their consequences on the population renewal of marine species. The aim of the present study was to analyse the effects of estuarine habitat degradation on the population of the common sole in the Eastern Channel, a key species in the fish community and fisheries in this area. We especially focused on the drastic drop in the surface area and on the low water quality of the Seine estuary, the main river of the Eastern Channel. A geographic Information System (GIS) was used to develop quantitative maps of sole nursery habitats in the Eastern Channel by using a habitat suitability model based on bathymetry and sediment structure. This approach indicated that juvenile densities are low in the Seine estuary with regards to other nursery sectors. Then, thanks to historical maps of the Seine estuary, habitat suitability maps were built for key dates in the modifications of this estuary since 1850. This backward predictive approach suggests that habitat loss in the Seine estuary has led to a 42% decrease of its nursery capacity. As the density of juvenile sole in the Seine estuary is low in comparison to other sectors, this represents only a 3% loss at the sole population scale, in the Eastern Channel. However, when we assumed that prior to anthropogenic disturbance the juvenile density in the Seine estuary might have been equivalent to the current density of adjacent sectors with higher quality, the loss in abundance could be nearly 23% (8-36%). Results suggest that the loss in habitat surface combined with habitat degradation has led to an important loss in the contribution of the Seine estuary nursery to the whole sole population in the Eastern Channel. (C) 2009 Elsevier BM. All rights reserved.
Journal Of Sea Research (1385-1101) (Elsevier Science Bv), 2010-07 , Vol. 64 , N. 1-2 , P. 34-44

Droits : 2009 Elsevier B.V. All rights reserved.
http://archimer.ifremer.fr/doc/00008/11921/9799.pdf
DOI:10.1016/j.seares.2009.08.003
http://archimer.ifremer.fr/doc/00008/11921/

Éditeur(s) : HAL CCSD American Physiological Society
Résumé : International audience
Zidi-Yahiaoui N, Callebaut I, Genetet S, Le Van Kim C, Cartron JP, Colin Y, Ripoche P, Mouro-Chanteloup I. Functional analysis of human RhCG: comparison with E. coli ammonium transporter reveals similarities in the pore and differences in the vestibule. Am J Physiol Cell Physiol 297: C537–C547, 2009. First published June 24, 2009; doi:10.1152/ajpcell.00137.2009.—Rh glycoproteins are members of the ammonium transporter (Amt)/methylamine permease (Mep)/Rh family facilitating movement of NH3 across plasma membranes. Homology models constructed on the basis of the experimental structures of Escherichia coli AmtB and Nitrosomonas europaea Rh50 indicated a channel structure for human RhA (RhAG), RhB (RhBG), and RhC (RhCG) glycoproteins in which external and internal vestibules are linked by a pore containing two strictly conserved histidines. The pore entry is constricted by two highly conserved phenylalanines, “twin-Phe.” In this study, RhCG function was investigated by stopped-flow spectrofluorometry measuring kinetic pH variations in HEK293E cells in the presence of an ammonium gradient. The apparent unitary NH3 permeability of RhCG was determined and was found to be close to that of AmtB. With a site-directed mutagenesis approach, critical residues involved in Rh NH3 channel activity were highlighted. In the external vestibule, the importance of both the charge and the conformation of the conserved aspartic acid was shown. In contrast to AmtB, individual mutations of each phenylalanine of the twin-Phe impaired the function while the removal of both resulted in recovery of the transport activity. The impact of the mutations suggests that, although having a common function and a similar channel structure, bacterial AmtB and human Rh vary in several aspects of the NH3 transport mechanisms.
ISSN: 0363-6143

hal-00417492
https://hal.archives-ouvertes.fr/hal-00417492
DOI : 10.1152/ajpcell.00137.2009.

Éditeur(s) : HAL CCSD Springer-Verlag Berlin Heidelberg
Résumé : International audience
The main biological function of reaction wood is to act as " muscle " for trees, enabling them to control their posture. The key property to achieve this function is the development of high mechanical stress during the formation of reaction wood cells, called " maturation strains ". Actually, reaction wood formation is basically the asymmetric formation of wood around the tree circumference, with higher maturation strains on the side where reaction wood is formed than on the opposite side. This asymmetry enables stems to bend upward or to compensate for the downward bending induced by gravity. At the cross section level, the performance in this biological function is linked not only to the magnitude of this asymmetry but also to an effect of the cross-sectional size (diameter) of the stem. Eccentric growth and variations in wood mechanical stiffness are second order effects that can modify this performance. Differences in maturation strains between reaction and non-reaction woods are related to their specific cell wall structure and composition. The swelling of the cell wall matrix during maturation and the effect of microfibril angle explain the differences in maturation strains between normal and compression wood. However, this mechanism fails in explaining the high maturation shrinkage of tension wood, and several hypotheses at the molecular levels are still under debate. How trees perceive their gravitational disequilibrium is also an open question for physiologists. Integrative biomechanical modelling (from the polymer level to the cell wall, cross section and whole tree levels) enables defining key variables that explain the performance of reaction wood as a system that insures the stem motricity. Maturation strains can be precisely measured only in recently formed wood at the tree surface, but their changes during the whole tree life can also be estimated by retrospective dendrochronological analysis through structural markers of reaction wood. Lastly, wood in living trees ensures general storage, defence, vascular and skeletal functions, that ask general questions about synergies and trade-offs as the structural characteristics of reaction wood can affect all these functions.
The Biology of Reaction Wood

ISBN : 978-3-642-10813-6
hal-01452085
https://hal.archives-ouvertes.fr/hal-01452085
https://hal.archives-ouvertes.fr/hal-01452085/document
https://hal.archives-ouvertes.fr/hal-01452085/file/Chap_Gril_al_Biomechanical_action_biological_functions_2014.pdf

Éditeur(s) : HAL CCSD Elsevier
Résumé : International audience
This work shows the preparation and separation performance assessment of carbon membranes derived from phenolic resin by a vacuum-assisted method and carbonisation in an inert atmosphere. The vacuum time played an important role in tailoring the structure of the membranes. For instance, pore volumes and surface areas increased from 0.81 and 834 to 2.2 cm3g−1and 1910 m2g−1, respectively, as the vacuum time exposure increased from 0 to 1200 s. The significant structural changes correlated very well with water permeation, as fluxes increased by 91% as the vacuum time increased from 0 to 1200 s reaching up to 169 L m−2h−1at 5 bar. Molecular weight cut-off tests showed no rejection for the smaller glucose and sucrose molecules, though this increased to ~ 80% and full rejection for 36 kDa and 400 kDa polyvinyl pyrrolidine. Interestingly, FTIR spectra showed that the peaks of C–H stretching vibration (2800–3200 cm−1) and C–O stretching (1030 cm−1) became more pronounced as a function of increasing vacuum time, strongly suggesting that the use of vacuum further assisted in the polycondensation of phenolic oligomers. Based on these outcomes, a cluster to cluster model is proposed, whereby vacuum application promoted crosslinking reactions of the phenolic resin, creating microporous regions within the clusters, and mesoporous regions between the clusters. © 2016 Elsevier B.V
ISSN: 0376-7388

hal-01683220
https://hal.univ-antilles.fr/hal-01683220
DOI : 10.1016/j.memsci.2016.11.002

Éditeur(s) : HAL CCSD Springer Verlag
Résumé : International audience
A hallmark of living systems is the management and the storage of information through genetic and epigenetic mechanisms. Although the notion of epigenetics was originally given to any regulation beyond DNA sequence, it has often been restricted to chromatin modifications, supposed to behave as cis-markers, specifying the sets of genes to be expressed or repressed. This definition does not take into account the initial view of epigenetics, based on nonlinear interaction networks whose "attractors" can remain stable without need for any chromatin mark. In addition, most chromatin modifications are the steady state resultants of highly dynamic modification and de-modification activities and, as such, seem poorly appropriate to work as long-term memory keepers. Instead, the basic support of epigenetic memory could remain the attractors, to which chromatin modifications belong as do many other components. The influence of chromatin modifications in memory is highly questionable when envisioned as static structural marks, but can be recovered under the dynamic circuitry perspective, thanks to their self-templating properties. Beside their standard repressive or permissive functions, chromatin modifications can also influence transcription in multiple ways such as: (1) by randomizing or inversely stabilizing gene expression, (2) by mediating cooperativity between pioneer and secondary transcription factors, and (3) in the hysteresis and the ultrasensitivity of gene expression switches, allowing the cells to take unambiguous transcriptional decisions.
ISSN: 1420-682X

hal-01063617
https://hal.archives-ouvertes.fr/hal-01063617
DOI : 10.1007/s00018-012-0923-7
PUBMED : 22331281

Éditeur(s) : HAL CCSD Wiley-Blackwell
Résumé : International audience
AIMS: Virus detection has often been difficult due to a low concentration in water. In this study, we developed a new procedure based on concentration of virus particles on an innovative support: poly-L-lysine dendrigrafts (DGL), coupled with directed nucleic acid extraction and real-time PCR quantification. METHODS AND RESULTS: This method was evaluated using the bacteriophage MS2 as a model virus. This virus exhibited the size and structural properties of human pathogenic enteric viruses and has often been used to assess new supports of concentration. Moreover, this bacteriophage is also a faecal contamination indicator. In this study, many water filtration conditions were tested (volume of water, concentration, etc.), and more than 80% of bacteriophage were recovered after filtration on polymer, in most conditions. We demonstrated that the method was linear (slope = 0*99 ± 0*04 and Y intercept when x = -0*02 ± 0*28), valid (as manipulators, tested concentrations, volumes of sample and batch of polymer did not have any influence on concentration) and sensitive (allowing to concentrate up to 16,600-fold 1 l of sample and to detect and quantify down to 750 GC l(-1) and 7500 GC l(-1), respectively). CONCLUSIONS: To conclude, this support exhibits high interest to retain viruses and to allow to detect low concentration of virus in water. SIGNIFICANCE AND IMPACT OF THE STUDY: This study gives valuable advance in the methods of concentration and diagnosis of virus in water.
Journal of Applied Microbiology

hal-00828987
https://hal.archives-ouvertes.fr/hal-00828987
DOI : 10.1111/jam.12209
PUBMED : 23551794

Éditeur(s) : HAL CCSD Springer Verlag
Résumé : International audience
In this work, we have studied quenching of the fluorescence of two well-known oxygen probes, 1-pyrene butyric acid (PBA) and tris(2,2'-bipyridine)ruthenium ([Ru(bpy)(3)](2+)) by reactive oxygen species (superoxide anion, nitric oxide derivative, hydrogen peroxide) and by the O(2) molecule. Both, time-resolved and steady state fluorescence measurements were performed in solution (ethanol, dimethyl sufoxide, water) and in micelles of Sodium Dodecyl Sulfate that serve as a model for membrane-containing biological structures. We have found that only the free radicals and O(2) can actively quench for the two probes, but not the diamagnetic H(2)O(2). Our data correspond to the classical Stern-Volmer equation. H(2)O(2) has an effect only at high molar concentrations (>0.1 M). In contrast, effective concentrations of free radicals and O(2) that lead to quenching are in millimolar range. In conclusion, our methods allows for detecting global ROS that are small free radicals without interference from the reactive hydroxyl radical. Our data suggest that the method can be used for the quantification of ROS in individual living cells based on the measurement of fluorescence lifetime of those probes
ISSN: 1053-0509

hal-01326234
https://hal.archives-ouvertes.fr/hal-01326234
DOI : 10.1007/s10895-008-0425-z

Éditeur(s) : HAL CCSD
Résumé : International audience
In the network modeling area, the most widely used definition of a "pattern" is that of a subgraph, a notion that considers only the network topological structure. While this definition has been very useful for extracting subgraphs frequently found in a network or a set of networks, it does not take into account the node attributes, an intrinsic component of social networks that often provides relevant information on the role or the position of a node in a network. In this paper, we propose a novel approach for extracting frequent patterns in social networks, called frequent link mining, based on the search for particular patterns that combine information on both network structure and node attributes. This kind of patterns, that we call frequent links, provides knowledge on the groups of nodes connected in the social network. In this article, we detail the method proposed for extracting frequent links and discuss its flexibility and its complexity. We demonstrate the efficiency of our solution by carrying out qualitative and quantitative studies.
Advances in Database (Weston, Conn.)s and Information Systems
Poznań, Poland

hal-00767050
https://hal.archives-ouvertes.fr/hal-00767050
DOI : 10.1007/978-3-642-33074-2_28

Éditeur(s) : HAL CCSD Elsevier
Résumé : International audience
Evidence of anomalous volcanism is readily observed in the Gulf of Aden, although, much of this oceanic basin remains as yet unmapped. In this paper, we investigate the possible connection of the Afar hotspot with a major off-axis volcanic structure and its interpretation as a consequence of a the anomalous presence of melt by integrating several data sets, both published and unpublished, from the Encens-Sheba cruise, the Aden New Century (ANC) cruise and several other onshore and marine surveys. These include bathymetric, gravity, magnetic, magneto-telluric data, and rock samples. Based upon these observations, interpretations were made of seafloor morphology, gravity and magnetic models, seafloor age, geochemical analyses and tectonic setting. We discuss the possible existence of a regional melting anomaly in the Gulf of Aden area and of the probability of its connection to the Afar plume. Several models that might explain the anomalous volcanism are taken into account, such as a local melting anomaly unrelated to the Afar plume, an anomalously large volume of melt associated with seafloor spreading, and interaction of the ridge with the Afar plume. A local melting anomaly and atypical seafloor spreading prove inconsistent with our observations. Two previously proposed models of plume-ridge interactions are examined: the diffuse plume dispersion called pancaked flow and channelized along-axis flow. We conclude that the configuration and structure of this young ocean basin may have the effect of channeling material away from the Afar plume along the Aden and Sheba Ridges to produce the off-axis volcanism observed on the ridge flanks. This interpretation implies that the influence of the Afar hotspot may extend much farther eastwards into the Gulf of Aden than previously believed. The segmentation of the Gulf of Aden and the configuration of the Aden-Sheba system may provide a potential opportunity to study channeled flow of solid plume mantle from the plume along a segmented ridge and nearby continental margins.
ISSN: 0012-821X

hal-00486140
https://hal.archives-ouvertes.fr/hal-00486140
DOI : 10.1016/j.epsl.2010.02.036

Éditeur(s) : HAL CCSD American Geophysical Union
Résumé : International audience
The anisotropic single crystal seismic properties are reviewed in the light of recent experimental and theoretical determinations. Although considerable progress has been made on the determination of single crystal properties, data are still lacking, particularly for the temperature derivatives of transition zone and lower mantle phases. The common types of LPO of olivine, opx and cpx are presented together with their associated seismic properties. It is emphasized that simple seismic symmetry pattern of upper mantle rocks are a direct result of the interaction of olivine, opx and cpx. Using the standard structural frame (X lineation, Z pole to foliation) for typical LPOs of olivine, opx and cpx have the maximum Vp parallel to X, in the XZ plane and parallel to Y respectively. Destructive interference occurs between these minerals and hence P-wave anisotropy should be sensitive to the aggregate composition. For shear wave splitting (dVs) typical olivine and opx LPOs result in similar patterns with the maximum dVs in the YZ plane and the fast split shear wave (Vs1) polarized parallel to the foliation. A typical cpx LPO on the other hand produces destructive interference as the max dVs is close to X. By comparison with experiments and numerical simulations, it is estimated that upper mantle samples have an olivine LPO strength which recorded shear strain gamma of between 0.25 and 2.0. Pyrolite and piclogite models are compared with global transverse isotropic models. The slowly reducing P-wave anisotropy in the first 200 km can be explained by a model with constant composition and LPO strength. The sharp decrease in the observed anisotropy in the global models cannot be explained by the transformation of opx to cpx at 300 km, it is proposed that this decrease is due to a reduction in LPO strength from 200 to 350 km at the base of the lithosphere.
Earth's Deep Interior: Mineral Physics and Tomography From the Atomic Scale to the Global Scale

ISBN : 9780875909752
hal-01391524
http://hal.univ-reunion.fr/hal-01391524
http://hal.univ-reunion.fr/hal-01391524/document
http://hal.univ-reunion.fr/hal-01391524/file/Mainprice_AGUmonog_DM_GB_WBI_2000.pdf

Éditeur(s) : HAL CCSD Elsevier
Résumé : International audience
We compile field data collected along the eastern part of the North Pyrenean Zone (NPZ) to point to a tectonic evolution under peculiar thermal conditions applying to the basin sediments in relation with the opening of the Cretaceous Pyrenean rift. Based on this compilation, we show that when thinning of the continental crust increased , isotherms moved closer to the surface with the result that the brittle-ductile transition propagated upward and reached sediments deposited at the early stage of the basin opening. During the continental breakup, the pre-rift Mesozoic cover was efficiently decoupled from the Paleozoic basement along the Triassic evaporite level and underwent drastic ductile thinning and boudinage. We suggest that the upper Albian and upper Creta-ceous flysches acted as a blanket allowing temperature increase in the mobile pre-rift cover. Finally, we show that continuous spreading of the basin floor triggered the exhumation of the metamorphic, ductily sheared pre-rift cover, thus contributing to the progressive thinning of the sedimentary pile. In a second step, we investigate the detailed geological records of such a hot regime evolution along a reference-section of the eastern NPZ. We propose a balanced restoration from the Mouthoumet basement massif (north) to the Boucheville Albian basin (south). This section shows a north to south increase in the HT Pyrenean imprint from almost no metamorphic recrystallization to more than 600 °C in the pre-and syn-rift sediments. From this reconstruction, we propose a scenario of tectonic thinning involving the exhumation of the pre-rift cover by the activation of various detachment surfaces at different levels in the sedimentary pile. In a third step, examination of the architecture of current distal passive margin domains provides confident comparison between the Pyrenean case and modern analogs. Finally, we propose a general evolutionary model for the pre-rift sequence of the Northeastern Pyrenean rifted margin.
ISSN: 0040-1951

Droits : http://creativecommons.org/licenses/by-nc-nd/
insu-01355131
https://hal-insu.archives-ouvertes.fr/insu-01355131
https://hal-insu.archives-ouvertes.fr/insu-01355131/document
https://hal-insu.archives-ouvertes.fr/insu-01355131/file/TECTO10807-Incorporated%20changes-rev2.pdf
DOI : 10.1016/j.tecto.2016.07.022

Éditeur(s) : HAL CCSD American Geophysical Union (AGU)
Résumé : International audience
The Ronda peridotite supplies one of the best objects to document subcontinental mantle deformation, but its internal deformation and exhumation mechanisms remain controversial. Here we provide new structural data and numerical results that constrain the Oligocene-Miocene deformation history of the Ronda massif. We first describe a mantle shear zone in the northern massif that developed subcrustal strain localization during decompression and related partial melting. The deformation regime of this mantle shear zone evolved from penetrative NE-SW stretching to sinistral shear highlighted by discrete shear bands. We then show structural observations that document a viscous deformation in the southern massif occurring prior to the thrust-assisted emplacement of the peridotite during large decompression. Finally, we performed numerical investigations that quantify a high temperature of 980°C for the basal peridotite lens at the time of its crustal emplacement. Our numerical results constrain the timing of ductile deformation of the peridotite just before 22 Ma, probably between 30 and 22 Ma. Altogether, these features led us to conclude that the deformation and exhumation of the Ronda peridotite results from lithosphere thinning subsequently inverted in the course of the Oligocene-Miocene. Among available models, our findings support the hypothesis of peridotite exhumation by the inversion of a thinned back-arc continental lithosphere during westward slab rollback through the Alboran region.
ISSN: 0278-7407

insu-00924180
https://hal-insu.archives-ouvertes.fr/insu-00924180
https://hal-insu.archives-ouvertes.fr/insu-00924180/document
https://hal-insu.archives-ouvertes.fr/insu-00924180/file/tect20062.pdf
DOI : 10.1002/tect.20062

Éditeur(s) : HAL CCSD Wiley-Blackwell
Résumé : International audience
Virus detection has often been difficult due to a low concentration in water. In this study, we developed a new procedure based on concentration of virus particles on an innovative support: poly-L-lysine dendrigrafts (DGL), coupled with directed nucleic acid extraction and real-time PCR quantification. Methods and Results: This method was evaluated using the bacteriophage MS2 as a model virus. This virus exhibited the size and structural properties of human pathogenic enteric viruses and has often been used to assess new supports of concentration. Moreover, this bacteriophage is also a faecal contamination indicator. In this study, many water filtration conditions were tested (volume of water, concentration, etc.), and more than 80% of bacteriophage were recovered after filtration on polymer, in most conditions. We demonstrated that the method was linear (slope = 0 99 0 04 and Y intercept when x = 0 02 0 28), valid (as manipulators, tested concentrations, volumes of sample and batch of polymer did not have any influence on concentration) and sensitive (allowing to concentrate up to 16 600-fold 1 l of sample and to detect and quantify down to 750 GC l 1 and 7500 GC l 1, respectively). Conclusions: To conclude, this support exhibits high interest to retain viruses and to allow to detect low concentration of virus in water. Significance and Impact of the Study: This study gives valuable advance in the methods of concentration and diagnosis of virus in water.
Journal of Applied Microbiology

hal-00932328
https://hal.archives-ouvertes.fr/hal-00932328
DOI : 10.1111/jam.12209