Éditeur(s) : HAL CCSD Wiley
Résumé : The intensification of the hydrological cycle, with an observed and modeled increase in drought incidence and severity, underscores the need to quantify drought effects on carbon cycling and the terrestrial sink. FLUXNET, a global network of eddy covariance towers, provides dense data streams of meteorological data, and through flux partitioning and gap filling algorithms, estimates of net ecosystem productivity (FNEP), gross ecosystem productivity (P), and ecosystem respiration (R). We analyzed the functional relationship of these three carbon fluxes relative to evaporative fraction (EF), an index of drought and site water status, using monthly data records from 238 micrometeorological tower sites distributed globally across 11 biomes. The analysis was based on relative anomalies of both EF and carbon fluxes and focused on drought episodes by biome and climatic season. Globally P was ≈50% more sensitive to a drought event than R. Network-wide drought-induced decreases in carbon flux averaged −16.6 and −9.3 g C m−2 month−1 for P and R, i.e., drought events induced a net decline in the terrestrial sink. However, in evergreen forests and wetlands drought was coincident with an increase in P or R during parts of the growing season. The most robust relationships between carbon flux and EF occurred during climatic spring for FNEP and in climatic summer for P and R. Upscaling flux sensitivities to a global map showed that spatial patterns for all three carbon fluxes were linked to the distribution of croplands. Agricultural areas exhibited the highest sensitivity whereas the tropical region had minimal sensitivity to drought. Combining gridded flux sensitivities with their uncertainties and the spatial grid of FLUXNET revealed that a more robust quantification of carbon flux response to drought requires additional towers in all biomes of Africa and Asia as well as in the cropland, shrubland, savannah, and wetland biomes globally.
ISSN: 1354-1013

hal-01032076
https://hal.archives-ouvertes.fr/hal-01032076
DOI : 10.1111/j.1365-2486.2009.01991.x

Éditeur(s) : HAL CCSD Nature Publishing Group
Résumé : International audience
Temperature and fluid pressure conditions control rock deformation and mineralization on geological faults, and hence the distribution of earthquakes1. Typical intraplate continental crust has hydrostatic fluid pressure and a near-surface thermal gradient of 31 ± 15 degrees Celsius per kilometre2, 3. At temperatures above 300–450 degrees Celsius, usually found at depths greater than 10–15 kilometres, the intra-crystalline plasticity of quartz and feldspar relieves stress by aseismic creep and earthquakes are infrequent. Hydrothermal conditions control the stability of mineral phases and hence frictional–mechanical processes associated with earthquake rupture cycles, but there are few temperature and fluid pressure data from active plate-bounding faults. Here we report results from a borehole drilled into the upper part of the Alpine Fault, which is late in its cycle of stress accumulation and expected to rupture in a magnitude 8 earthquake in the coming decades4, 5. The borehole (depth 893 metres) revealed a pore fluid pressure gradient exceeding 9 ± 1 per cent above hydrostatic levels and an average geothermal gradient of 125 ± 55 degrees Celsius per kilometre within the hanging wall of the fault. These extreme hydrothermal conditions result from rapid fault movement, which transports rock and heat from depth, and topographically driven fluid movement that concentrates heat into valleys. Shear heating may occur within the fault but is not required to explain our observations. Our data and models show that highly anomalous fluid pressure and temperature gradients in the upper part of the seismogenic zone can be created by positive feedbacks between processes of fault slip, rock fracturing and alteration, and landscape development at plate-bounding faults.
ISSN: 0028-0836

hal-01685757
https://hal.archives-ouvertes.fr/hal-01685757
DOI : 10.1038/nature22355

Éditeur(s) : HAL CCSD European Geosciences Union
Résumé : The fixation of carbon in tropical forests mainly occurs through the production of wood and leaves, both being the principal components of net primary production. Currently field and satellite observations are independently used to describe the forest carbon cycle, but the link between satellite-derived forest phenology and field-derived forest productivity remains opaque. We used a unique combination of a MODIS enhanced vegetation index (EVI) dataset, a wood production model based on climate data and direct litterfall observations at an intra-annual timescale in order to question the synchronism of leaf and wood production in tropical forests. Even though leaf and wood biomass fluxes had the same range (respectively 2.4 +/- 1.4 and 2.2 +/- 0.4 Mg C ha(-1) yr(-1)), they occurred separately in time. EVI increased with leaf renewal at the beginning of the dry season, when solar irradiance was at its maximum. At this time, wood production stopped. At the onset of the rainy season, when new leaves were fully mature and water available again, wood production quickly increased to reach its maximum in less than a month, reflecting a change in carbon allocation from short-lived pools (leaves) to long-lived pools (wood). The time lag between peaks of EVI and wood production (109 days) revealed a substantial decoupling between the leaf renewal assumed to be driven by irradiance and the water-driven wood production. Our work is a first attempt to link EVI data, wood production and leaf phenology at a seasonal timescale in a tropical evergreen rainforest and pave the way to develop more sophisticated global carbon cycle models in tropical forests.
ISSN: 1726-4170

hal-01268497
https://hal.archives-ouvertes.fr/hal-01268497
DOI : 10.5194/bg-10-7307-2013
PRODINRA : 256018

Éditeur(s) : HAL CCSD Elsevier
Résumé : This paper is the first presentation of a project called GHYRAF (Gravity and Hydrology in Africa) devoted to the detailed comparison between models and multidisciplinary observations (ground and satellite gravity, geodesy, hydrology, meteorology) of the variations of water storage in Africa from the Sahara and part to the monsoon equatorial part. We describe the various actions planned in this project. We first detail the actions planned in gravimetry which consist in two main surface gravity experiments: on the one hand the periodic repetition of absolute gravity measurements along a north-south monsoonal gradient of rainfall in West Africa, going from Tamanrasset (20 mm/year) in southern Algeria to Djougou (1200 mm/year) in central Benin; on the other hand the continuous measurements at Djougou (Benin) with a superconducting gravimeter to monitor with a higher sampling rate the gravity changes related to an extreme hydrological cycle. Another section describes the actions planned in GPS which will maintain and develop the present-day existing network in West Africa. The third type of actions deals with hydrology and we review the three sites that will be investigated in this joint hydrogeophysics project namely Wankama (near Niamey) and Bagara (near Diffa) in the Niger Sahelian zone and Nalohou (near Djougou) in the Benin monsoon zone. We also address the question of the ground truth of satellite-derived missions: in this context the GHYRAF project will lead to test the hydrology models by comparison both with in situ and satellite data such as GRACE, as well as to an important increase of our knowledge of the seasonal water cycle in Africa. We finally present preliminary results in GPS based on the analysis of the vertical motion of the Djougou site. The resulting absolute gravity changes related to the 2008 monsoon are finally given.
ISSN: 0264-3707

hal-00475660
https://hal.archives-ouvertes.fr/hal-00475660
DOI : 10.1016/j.jog.2009.09.014

Éditeur(s) : HAL CCSD Ecological Society of America
Résumé :  
The expansion of selective logging in tropical forests may be an important source of global carbon emissions. However, the effects of logging practices on the carbon cycle have never been quantified over long periods of time. We followed the fate of more than 60 000 tropical trees over 23 years to assess changes in aboveground carbon stocks in 48 1.56-ha plots in French Guiana that represent a gradient of timber harvest intensities, with and without intensive timber stand improvement (TSI) treatments to stimulate timber tree growth. Conventional selective logging led to emissions equivalent to more than a third of aboveground carbon stocks in plots without TSI (85 Mg C/ha), while plots with TSI lost more than one-half of aboveground carbon stocks (142 Mg C/ha). Within 20 years of logging, plots without TSI sequestered aboveground carbon equivalent to more than 80% of aboveground carbon lost to logging (-70.7 Mg C/ha), and our simulations predicted an equilibrium aboveground carbon balance within 45 years of logging. In contrast, plots with intensive TSI are predicted to require more than 100 years to sequester aboveground carbon lost to emissions. These results indicate that in some tropical forests aboveground carbon storage can be recovered within half a century after conventional logging at moderate harvest intensities.
Ecological Applications

hal-01032171
https://hal.archives-ouvertes.fr/hal-01032171

Éditeur(s) : HAL CCSD
Résumé : International audience
Symbiosis is a widespread phenomenon in the biosphere. In marine benthic environments breakdown of symbiosis is responsible for coral bleaching and dramatically affects coral reef ecosystems functioning [1]. In the water column, planktonic organisms are key component of pelagic ecosystems and a number of species form mutualistic association with microalgae forming a photosymbiosis [2]. Here we intended to investigate the genetic basis of photosymbiosis, through a transcriptomic approach on marine plankton organisms. We focused more specifically on associations occurring between radiolarian host (protist, zooplankton) and dinoflagellates symbiont (protist, phytoplankton) living inside the host cell. It has recently been highlighted that these holobionts are widespread in oligotrophic open oceans and there are also evidences of their fundamental implication in biogeochemical carbon, silica and strontium cycles [3, 4, 5, 6]. RNA-seq technologies allow obtaining an unprecedented amount of data for unicellular organisms isolated from the environment [7]. The study of such non-model holobionts datasets requires de novo assembly, which implies considerable computational resources and the potential production of chimeric sequences [8, 9].We therefore developed an original strategy aiming at accelerating and improving de novo assembly for holobiont datasets. We chose SIMKA [10] a fast kmer-based method initially developed to estimate the similarity between numerous metagenomic datasets, and which has been recently adapted to extract their common sequences. As our symbionts are identified, we used SIMKA to compare our holobionts transcriptomes to publicly available dinoflagellates transcriptomes [11] generating two datasets, one composed of reads from the symbionts and another with reads from the host (for which no reference data are currently available). Independent assemblies were then performed in parallel, accelerating the study process, and minimizing the proportion of resulting chimeric sequences. Our strategy produced a unique and comprehensive genomic dataset for Radiolaria [12, 13], and offers a pragmatic, large scale, comparison strategy to assemble and study holobionts [9]. Our new sequences obtained from holobionts study will be used for phylogenomics investigation, as reference for environmental metagenomic studies and ultimately to understand and characterize the molecular basis of symbiotic relationships in the plankton.Bibliografic references :[1] Simon K. Davy, Denis Allemand, and Virginia M. Weis. “Cell Biology of Cnidarian-Dinoflagellate Symbiosis”. In: Microbiology and Molecular Biology Reviews 76.2 (June 1, 2012), pp. 229–261. issn: 1092-2172, 1098- 5557. doi: 10.1128/MMBR.05014-11.[2] Janouškovec, J. et al. “Major transitions in dinoflagellate evolution unveiled by phylotranscriptomics”. in: PNAS 201614842 (2016). doi:10.1073/pnas.1614842114[3] Tristan Biard et al. “In situ imaging reveals the biomass of giant protists in the global ocean”. In: Nature advance online publication (Apr. 20, 2016). issn: 0028-0836. doi: 10.1038/nature17652.[4] Lionel Guidi et al. “Plankton networks driving carbon export in the olig- otrophic ocean”. In: Nature 532.7600 (Apr. 28, 2016), pp. 465–470. issn: 0028-0836. doi: 10.1038/nature16942.[5] Diane K. Stoecker et al. “Acquired phototrophy in aquatic protists”. In: Aquatic Microbial Ecology 57.3 (Nov. 24, 2009), pp. 279–310. doi: 10.3354/ame01340.[6] Johan Decelle et al. “An original mode of symbiosis in open ocean plankton”. In: Proceedings of the National Academy of Sciences 109.44 (Oct. 30, 2012), pp. 18000–18005. issn: 0027-8424, 1091-6490. doi: 10. 1073/pnas.1212303109.[7] Sergio Balzano et al. “Transcriptome analyses to investigate symbiotic relationships between marine protists”. In: Frontiers in Microbiology 6 (Mar. 17, 2015). issn: 1664-302X. doi: 10.3389/fmicb.2015.00098.[8] Li, B. et al. “Evaluation of de novo transcriptome assemblies from RNA-Seq data”. In: Genome Biology 15, 553 (2014).[9] Sangwan, N., Xia, F. & Gilbert, J. A. “Recovering complete and draft population genomes from metagenome datasets”. In: Microbiome 4, 8 (2016).[10] Gaëtan Benoit et al. “Multiple comparative metagenomics using mul- tiset k -mer counting”. In: PeerJ Computer Science 2 (Nov. 14, 2016). doi: 10.7717/peerj-cs.94.[11] Patrick J. Keeling et al. “The Marine Microbial Eukaryote Transcriptome Sequencing Project (MMETSP): Illuminating the Functional Diversity of Eukaryotic Life in the Oceans through Transcriptome Sequencing”. In: PLOS Biol 12.6 (June 2014), e1001889. issn: 1545-7885. doi: 10.1371/journal.pbio.1001889.[12] Burki, F. et al. “Evolution of Rhizaria: new insights from phylogenomic analysis of uncultured protists”. In BMC Evolutionary Biology 10, 377 (2010).
International Conference on Holobionts
Paris, France

hal-01575069
https://hal.inria.fr/hal-01575069

Éditeur(s) : HAL CCSD Elsevier
Résumé : International audience
To understand the deep cycle of water, upper mantle water content and distribution between nominally anhydrous minerals (NAMs) and hydrous minerals (e.g., amphibole) must be constrained. We need then to understand H behaviour during mantle melting and metasomatism. Major, minor and trace element compositions including water contents were obtained on ten xenoliths of spinel-bearing peridotites from the Ray Pic locality, in the Southern part of the Massif Central (France). The sample suite investigated here is composed of rather fertile lherzolites (89.4 ≤ Fo ≤ 90.8%; 11.3 ≤ cr# in spinel ≤ 21.1%; 0.942 ≤ [Yb]cpx ≤ 1.90 ppm; cpx: clinopyroxene), which can be best explained by batch melting, with degree of melting between 3 and 10%. These xenoliths contain up to 8% modal amphibole. Three groups are defined as a function of the amphibole modal abundance (above or below 1%) and equilibrium temperature (above or below 1000 °C). Results show no clear positive correlation between modal metasomatism (amphibole) and incompatible element enrichment in cpx. Trace element compositions in cpx show strong enrichments of the most incompatible elements (e.g., (La/Sm)PM as high as 15.7; PM: normalised to primitive mantle values), but strong negative anomalies of the high field strength elements (e.g., (Th/Nb)PM as high as ~ 680). Such trace element fractionations are usually ascribed to the so-called carbonatitic metasomatism involving the percolation of small volume melts which are enriched in volatiles. The hydrogen concentrations in cpx range from 203 to 330 ppm wt. H2O, in orthopyroxene from 66 to 160 ppm wt. H2O and in olivine from 2 to 6 ppm wt. H2O. These values are within the common concentration range of other spinel-bearing peridotites. Amphiboles contain 1.9 ± 0.5 wt.% of H2O.The effect of metasomatism on water abundances in NAMs is not straightforward. Hydrous metasomatism (i.e., leading to the crystallisation of OH-bearing amphibole) has no effect on the water content of the co-existing NAMs. This suggests thus that the occurrence of hydrous minerals, such as amphibole, does not systematically imply that the coexisting NAMs are water-rich (saturated). Further, the percolation of volatile-rich small volume melts, which is fingerprinted by the strong enrichment of the incompatible elements, has also no clear effect on the water content of the NAMs. These data are thus difficult to reconcile with the admitted highly incompatible behaviour of H in upper mantle minerals.
ISSN: 0024-4937

hal-01278062
https://hal.archives-ouvertes.fr/hal-01278062
DOI : 10.1016/j.lithos.2015.08.013

Résumé : En 1999, le risque écologique encouru par les écosystèmes côtiers soumis à la prolifération de la crépidule, Crepidula fornicata, a été pleinement reconnu par la communauté scientifique nationale et internationale. Au niveau national, le Programme National Environnement Côtier (PNEC) et le programme national LITEAU ont tous deux identifié des actions thématiques prioritaires sur la dynamique de prolifération de la crépidule et les interactions crépidule/bivalves exploités. Les actions du LEMAR (UMR 6539 CNRS) sur cette thématique s’inscrivent dans deux projets : 1 - La crépidule : identifier les mécanismes de sa prolifération et caractériser ses effets sur le milieu pour envisager sa gestion. » (LITEAU) 2 - Recrutement, milieu physique et interactions d'espèces en environnement côtier : cas des mollusques » (PNEC ART2) En rade de Brest, l’accent est mis sur l’interaction des cycles biologiques de la crépidule et de la coquille Saint-Jacques. Les objectifs poursuivis en rade de Brest s’articulent en 4 points, dans le cadre de LITEAU : 1- Dynamique de colonisation de la crépidule en rade de Brest : acquisition des paramètres de dynamique de population (caractéristiques du cycle reproducteur, déterminisme du recrutement, dispersion, croissance, sensibilité aux efflorescences toxiques...) ; 2- Impact de cette prolifération sur l’évolution de la nature sédimentaire des zones colonisées et l’enrichissement en matière organique (par le biais de l’activité alimentaire et de la production de biodépôts) ; 3- Modifications engendrées sur les peuplements benthiques dans leur globalité (sites ateliers). La strate à crépidules, au sens biosédimentaire et écologique, verra sa définition affinée et sa dynamique analysée en vue d'obtenir un modèle prévisionnel des évolutions possibles en matière de biodiversité ; 4- Rôle de la crépidule dans le contrôle de la production primaire phytoplanctonique (par le biais des activités de filtration et de production de biodépôts).

Droits : info:eu-repo/semantics/openAccess
http://archimer.ifremer.fr/doc/00032/14292/11570.pdf
http://archimer.ifremer.fr/doc/00032/14292/

Éditeur(s) : HAL CCSD
Résumé : The spectrin-based skeleton is an essential scaffold underlying different lipid bilayers in all animal cells. Spectrins (Sp), as giant extended flexible heterotetramers (αβ)2, constitute the filaments of this network, the nodes of which are cross-linked by actin filaments. The functions of the Sp-based skeleton, which are well defined in red blood cells (required for shape, resistance to shear stress and deformability) are less well understood in non-erythroid cells.The non-erythroid Sp might participate in the establishment and/or the maintenance of specialized membrane subdomains by accumulating integral membrane proteins (such as ionic channels, ionic pumps, receptors and cell adhesion molecules) as revealed by loss of the main non-erythroid β-Sp. However the effect of non-erythroid α-Sp are less well established. In mammals, α-Sp are encoded by two genes: one for αI-Sp mainly expressed in mature erythrocyte, the second one for αII-Sp which is expressed in all nucleated cells.In the pursuit of αII-Sp functions, we have checked the effect of αII-Sp knock down by siRNA melanoma human cell line. Our data revealed that spectrin depletion is associated with i) an arrest of cell cycle at G1 state ii) a loss of cell adhesion in spite of increase in some integrins iii) some modifications of actin organisation.
Le squelette dépendant de la spectrine, localisé sous la bicouche lipidique, est un échafaudage essentiel de toutes les cellules animales. La Spectrine (Sp), structure géante, robuste mais flexible d'hétéro tétramères (αβ)2, constitue les filaments de ce réseau, dont chaque nœud interagit avec des filaments d'actine. Les fonctions du squelette de Sp, qui sont bien définies dans le globule rouge (rôle dans sa forme, dans sa résistance aux forces de cisaillement et dans sa déformabilité) sont moins bien connues dans les cellules non-érythroïdes. La Sp non-érythroïde pourrait participer à l'établissement et/ou au maintien de sous-domaines membranaires spécialisés dans l'accumulation de protéines de membrane (telles que les canaux ioniques, les pompes ioniques, les récepteurs et les molécules d'adhérence) comme nous le font penser les invalidations de la Sp-β non-érythroïde. Cependant le rôle de la Sp-α non-érythroïde est moins bien établi. Chez les mammifères, la Sp-α est codée par deux gènes: un pour Sp-αI, principalement exprimée dans l'érythrocyte mature, le second pour Sp-αII qui est exprimée dans toutes les cellules nucléées. Pour étudier les fonctions de la Sp-αII, nous avons utilisé une approche de SiRNA sur des cellules de mélanome humain. Nos données montrent que la diminution de Sp-αII est associée à I) un arrêt en G1 du cycle cellulaire II) une perte d'adhérence des cellules malgré l'augmentation de quelques intégrines III) des modifications dans l'organisation du réseau d'actine.
https://tel.archives-ouvertes.fr/tel-00377569

Droits : info:eu-repo/semantics/OpenAccess
tel-00377569
https://tel.archives-ouvertes.fr/tel-00377569
https://tel.archives-ouvertes.fr/tel-00377569/document
https://tel.archives-ouvertes.fr/tel-00377569/file/these_210409_entiere.pdf
https://tel.archives-ouvertes.fr/tel-00377569/file/II-Spectrin_Is_Critical_for_Cell_Adhesion_and_Cell_Cycle.PDF

Éditeur(s) : HAL CCSD BioMed Central
Résumé : International audience
Cell proliferation is a hallmark of cancer and depends on complex signaling networks that are chiefly supported by protein kinase activities. Therapeutic strategies have been used to target specific kinases but new methods are required to identify combined targets and improve treatment. Here, we propose a small interfering RNA genetic screen and an integrative approach to identify kinase networks involved in the proliferation of cancer cells. The functional siRNA screen of 714 kinases in HeLa cells identified 91 kinases implicated in the regulation of cell growth, most of them never being reported in previous whole-genome siRNA screens. Based on gene ontology annotations, we have further discriminated between two classes of kinases that, when suppressed, result in alterations of the mitotic index and provoke cell-cycle arrest. Extinguished kinases that lead to a low mitotic index mostly include kinases implicated in cytosolic signaling. In contrast, extinguished kinases that result in a high mitotic index mostly include kinases implicated in cell division. By mapping hit kinases in the PhosphPOINT phosphoprotein database, we generated scale-free networks consisting of 449 and 661 protein-protein interactions for kinases from low MI and high MI groups, respectively. Further analyses of the kinase interactomes revealed specific modules such as FER- and CRKL-containing modules that connect three members of the epidermal growth factor receptor (EGFR) family, suggesting a tight control of the mitogenic EGF-dependent pathway. Based on experimental studies, we confirm the involvement of these two kinases in the regulation of tumor cell growth. Based on a combined approach of large kinome-wide siRNA screens and ontology annotations, our study identifies for the first time two kinase groups differentially implicated in the control of cell proliferation. We further demonstrate that integrative analysis of the kinase interactome provides key information which can be used to facilitate or optimize target design for new therapeutic strategies. The complete list of protein-protein interactions from the two functional kinase groups will provide a useful database for future investigations.
ISSN: 1471-2164

hal-01118475
https://hal-univ-rennes1.archives-ouvertes.fr/hal-01118475
DOI : 10.1186/1471-2164-15-1169
PUBMED : 25540073

Éditeur(s) : HAL CCSD
Résumé : Background and objectives.[br/] Dairy propionibacteria are isolated from various ecological niches, including soil, grass, silage, rumen, raw milk and dairy plants, showing great adaptability and robustness. Used as ripening starters in Swiss type cheeses, these food grade bacteria are described as nutraceutical producers and they release into the external medium short chain fatty acids (SCFA), folic acid, cobalamin and the bifidogenic 1,4- dihydroxy-2-naphthoic acid (DHNA). These compounds are known to play a pivotal role in the modulation of intestinal physiology through diet. Considering the pro-apoptotic potential of some of these metabolites, we investigated the potential of dairy products fermented by the main species, Propionibacterium freudenreichii, to prevent colon carcinogenesis. Methods.[br/] We developed a new fermented milk. Taking advantage of milk fractions, high and stable populations of P. freudenreichii were obtained in this product. It was tested in vitro on gastric HGT-1 and colic HT-29 cells with respect to the typical hallmarks of apoptosis. In vivo, the fermented milk was evaluated in piglets with respect to general health parameters and in human microbiota-associated rats, treated or not with dimethylhydrazine (DMH), with respect to intestinal proliferation and apoptosis. Results. Fermented milk induced cell death in both gastric and colon cancer cells. Cellular and molecular characterization of cells dying in response to SCFA treatment revealed cell cycle arrest, drop in intracellular ATP, depolarization of mitochondria, translocation of key apoptosis proteins, processing of caspases and fragmentation of the nucleus. Interestingly, the new fermented milk was shown to potentiate the cytotoxic effect of molecules used in cancer chemotherapy. In accordance, it modulated expression of key apoptotic proteins. In vivo, the fermented milk was evaluated in piglets. Daily oral gavage led to high colic P. freudenreichii populations, to modulation of the intestinal microbiota and modulation of intestinal cytokines. Promotion of piglets’ growth also revealed general probiotic effects of fermented milk. Considering that propionibacteria were metabolically active in the intestine, we further investigated their impact on colon carcinogenesis (induced by dimethylhydrazine, DMH) in human microbiota-associated rats. Consumption of selected strains of propionibacteria resulted in enhanced apoptotic depletion of DMH damaged epithelial cells, yet had no effect on neither proliferation nor apoptosis in healthy rats. Conclusions and perspectives.[br/] These results open new perspectives in the field of colon cancer cells prevention and/or treatment. The synergy with pro-apoptotic chemotherapy molecules suggests that such a fermented product may be proposed as a food supplement to enhance the effects of anticancer treatments.
colloque international « Nutrition and Cancer : Hot topics from biology to public health issues »
Paris, France

hal-01190452
https://hal.archives-ouvertes.fr/hal-01190452
PRODINRA : 201853

Éditeur(s) : HAL CCSD Public Library of Science
Résumé : International audience
BACKGROUND: Gastric cancer is one of the most common cancers in the world. The "economically developed countries" life style, including diet, constitutes a risk factor favoring this cancer. Diet modulation may lower digestive cancer incidence. Among promising food components, dairy propionibacteria were shown to trigger apoptosis of human colon cancer cells, via the release of short-chain fatty acids acetate and propionate. METHODOLOGY/PRINCIPAL FINDINGS: A fermented milk, exclusively fermented by P. freudenreichii, was recently designed. In this work, the pro-apoptotic potential of this new fermented milk was demonstrated on HGT-1 human gastric cancer cells. Fermented milk supernatant induced typical features of apoptosis including chromatin condensation, formation of apoptotic bodies, DNA laddering, cell cycle arrest and emergence of a subG1 population, phosphatidylserine exposure at the plasma membrane outer leaflet, reactive oxygen species accumulation, mitochondrial transmembrane potential disruption, caspase activation and cytochrome c release. Remarkably, this new fermented milk containing P. freudenreichii enhanced the cytotoxicity of camptothecin, a drug used in gastric cancer chemotherapy. CONCLUSIONS/SIGNIFICANCE: Such new probiotic fermented milk may thus be useful as part of a preventive diet designed to prevent gastric cancer and/or as a food supplement to potentiate cancer therapeutic treatments.
ISSN: 1932-6203

inserm-00871491
http://www.hal.inserm.fr/inserm-00871491
http://www.hal.inserm.fr/inserm-00871491/document
http://www.hal.inserm.fr/inserm-00871491/file/Milk_Fermented_by_Propionibacterium_freudenreichii.accepted.pdf
DOI : 10.1371/journal.pone.0031892

Éditeur(s) : HAL CCSD Wiley
Résumé : International audience
In order to study the effect of a competitive triathlon season on maximal oxygen uptake (VO2max), aerobic power (AeP) and anaerobic performance (AnP) of the lower limbs, eight triathletes performed exercise tests after: (1) a pre-competition period (Pre-COMP) (2) a competitive period (COMP), and (3) a low (volume and intensity) training period (Post-COMP). The tests were a vertical jump-and-reach test and an incremental exercise test on a cycle ergometer. Ventilatory data were collected every minute during the incremental test with an automated breath-by-breath system and the heart-rate was monitored using a telemetric system. RESULTS: No changes in VO2max were observed, whereas AeP decreased after Post-COMP compared to Pre-COMP and COMP and AnP decreased during COMP compared to Pre-COMP and Post-COMP. In addition, second ventilatory threshold (VT2) and power output at first ventilatory threshold (VT1) and VT2 decreased after Post-COMP. CONCLUSION: This study showed that six weeks of low volume and intensity of training is too long a period to preserve adaptations to training, although a stable maximal oxygen uptake throughout the triathlon season was observed. Moreover, the AnP decrease during COMP was probably in relation with the repetitive nature of the training mode and/or triathlon competitions.
ISSN: 0905-7188

hal-00720811
https://hal.univ-antilles.fr/hal-00720811
https://hal.univ-antilles.fr/hal-00720811/document
https://hal.univ-antilles.fr/hal-00720811/file/Sms-10170.pdf

Résumé : Cette thèse concerne la modélisation des dynamiques de maladies foliaires de cultures pérennes tropicales à différentes échelles spatiales. Cette approche de modélisation est appliquée à la cercosporiose noire du bananier. Il s’agit d’explorer et de déterminer les paramètres environnementaux et de résistance de l’hôte ayant une influence significative sur la dynamique spatiotemporelle de la maladie et d’apporter des éléments associés au contrôle de la cercosporiose noire.Deux modèles ont été développés dans le cadre de cette thèse. La dynamique épidémiologique au niveau de la plante est décrite par un modèle mécaniste décomposé en un modèle de croissance de la plante et un module épidémiologique décrivant le cycle épidémique du pathogène. L’architecture du bananier est prise en compte par le biais de compartiments foliaires positionnés dans l’espace. Le modèle a été validé par un jeu de données indépendant. Les expérimentations numériques et l’analyse de sensibilité du modèle réalisées par les méthodes de Morris et de e-FAST ont permis de mieux comprendre le fonctionnement épidémique de cette maladie et d’identifier les paramètres influant le plus la dynamique épidémique en particulier la vitesse d’extension des lésions, la durée d’incubation et l’efficacité d’infection .L’approche bayésienne a permis de prendre en compte l’information a priori disponible pour ces trois paramètres sur lesquels a porté l’inférence statistique. L’analyse de sensibilité du modèle a également permis d’identifier l’influence de deux paramètres liés à la croissance de la plante : le nombre de feuilles sur une plante et le rythme d’émission foliaire Un modèle de dynamique spatio-temporelle de la cercosporiose noire a été développé à l’échelle d’un territoire à partir d’enquêtes effectuées enMartinique pendant la période d’invasion de cette maladie de septembre 2010 à mai 2012. Les données récoltées étant censurées, l’inférence des paramètres du modèle a été réalisée dans un cadre bayésien en utilisant un algorithme d’augmentation de données. Le modèle et l’inférence développés permettent de reconstruire la dynamique spatio-temporelle de l’invasion et de prédire la fin d’invasion sur le territoire.Les deux modèles de dynamiques spatio-temporelles développés à deux échelles spatiales différentes ont permis d’acquérir des informations importantes pour construire des outils de conception de méthode de contrôle de la cercosporiose noire des bananiers.
This thesis concerns the modeling of the dynamics of foliar diseases of tropical tree crops at different spatial scales. This modeling approach is applied to black Sigatoka of banana. It is to explore and determine the environmental parameters and host resistance has a significant influence on the spatiotemporal dynamics of the disease and to provide elements associated with the control of Sigatoka noire.Deux models were developed as part of this thesis. The epidemiological dynamics at the plant is described by a mechanistic model decomposed into a growth model of the plant and an epidemiological unit describing the pathogen epidemic cycle. The architecture of the banana is taken into account through foliar compartments positioned in space. The model was validated by an independent data set. Numerical experiments and model sensitivity analysis performed by the methods of Morris and e-FAST enabled to better understand the functioning of this epidemic disease and identify the parameters affecting the most dynamic epidemic especially speed extension of the lesions, the incubation period and efficiency of Bayesian .L'approche infection allowed to take into account prior information available for these three parameters that were the statistical inference. The model sensitivity analysis also identified the influence of two parameters related to plant growth: the number of leaves on a plant and rate of leaf emission A dynamic model of space-time Black Sigatoka has been developed at the scale of a territory from surveys enMartinique during the period of invasion of the disease from September 2010 to May 2012. The data collected being censored, inference of model parameters has was performed in a Bayesian framework, using a data augmentation algorithm. The model developed and inference possible to reconstruct the spatiotemporal dynamics of the invasion and predict the end of the invasion territoire.Les two spatio-temporal dynamics models developed at two different spatial scales has been gained important information for build tools design method of control of black Sigatoka of banana.

http://www.theses.fr/2015AGUY0835/document

Éditeur(s) : HAL CCSD ACSM
Résumé : International audience
A 26-yr-old man with sickle cell trait (SCT) suddenly lost visual acuity in the left eye after a cycling race in hot tropical environment. The cause was massive central retinal vein occlusion (CRVO) with hemorrhaging that rapidly worsened to neovascular glaucoma. Although medically treated, the eye is now marked by total retinal detachment. Cardiovascular function assessment shows no electrocardiographic abnormalities, no anomaly in the supra-aortic tree, and no evidence of structural heart disease. Although normal coagulation markers values (i.e., activated partial thromboplastin time, prothrombin time, fibrinogen concentration, antithrombin III, factor V, proteins C and S) were observed 2.5 months after the clinical event, a transesophageal echocardiogram performed few hours after the incident revealed the presence of four thrombi in the left atrium suggesting a postexercise hypercoagulable state at that time. Hemorheological measurements at distance of the events demonstrated high red blood cell rigidity at baseline. Therefore, marked blood rheological impairment and activation of the coagulation pathway in response to the heavy and prolonged cycling race could have promoted CRVO in this cyclist carrying SCT. These data suggest that SCT could be considered as a risk factor for significant ocular complications when severe exercise is performed and support the idea that SCT is a contributing factor in blood rheology and vascular dysfunctions.
Medicine & Science in Sports & exercise

Droits : info:eu-repo/semantics/OpenAccess
hal-00698572
https://hal.univ-antilles.fr/hal-00698572
https://hal.univ-antilles.fr/hal-00698572/document
https://hal.univ-antilles.fr/hal-00698572/file/Hedreville_et_al_revised_MSSE-D-08-00064R2.pdf
DOI : 10.1249/MSS.0b013e31818313d0

Éditeur(s) : HAL CCSD Société nationale de protection de la nature (France)
Résumé : International audience
Les plantes qui présentent des structures anatomiques permettant de retenir de l'eau en permanence sont assez répandues en milieu tropical. Si beaucoup sont maintenant cultivées pour être vendues en jardineries, faisant le bonheur des amateurs, elles forment en milieu naturel des écosystèmes aquatiques encore très peu étudiés et renferment une biodiversité que l'on est loin d'avoir recensée. En Amérique centrale et du Sud, les broméliacées-citerne, qui représentent les plus nombreuses et les plus diversifiées de ces " plantes-mares ", permettent à des organismes très variés d'accomplir leur cycle de vie
ISSN: 0011-0477

hal-00849049
https://hal.archives-ouvertes.fr/hal-00849049
https://hal.archives-ouvertes.fr/hal-00849049/document
https://hal.archives-ouvertes.fr/hal-00849049/file/Carrias2013_Des_plantes.pdf

Éditeur(s) : HAL CCSD Public Library of Science
Résumé : International audience
The capacity of testicular somatic cells to promote and sustain germ cell differentiation is largely regulated by sexual steroids and notably androgens. In fish species the importance of androgens is emphasized by their ability to induce sex reversal of the developing fries and to trigger spermatogenesis. Here we studied the influence of androgens on testicular gene expression in trout testis using microarrays. Following treatment of immature males with physiological doses of testosterone or 11-ketotestosterone, 418 genes that exhibit changes in expression were identified. Interestingly, the activity of testosterone appeared stronger than that of 11-ketotestosterone. Expression profiles of responsive genes throughout testis development and in isolated germ cells confirmed androgens to mainly affect gene expression in somatic cells. Furthermore, specific clusters of genes that exhibit regulation coincidently with changes in the natural circulating levels of androgens during the reproductive cycle were highlighted, reinforcing the physiological significance of these data. Among somatic genes, a phylogenetic footprinting study identified putative androgen response elements within the proximal promoter regions of 42 potential direct androgen target genes. Finally, androgens were also found to alter the germ line towards meiotic expression profiles, supporting the hypothesis of a role for the somatic responsive genes in driving germ cell fate. This study significantly increases our understanding of molecular pathways regulated by androgens in vertebrates. The highly cyclic testicular development in trout together with functions associated with regulated genes reveal potential mechanisms for androgen actions in tubule formation, steroid production, germ cell development and sperm secretion.
ISSN: 1932-6203

inserm-00825763
http://www.hal.inserm.fr/inserm-00825763
http://www.hal.inserm.fr/inserm-00825763/document
http://www.hal.inserm.fr/inserm-00825763/file/Rolland_et_al.pdf
DOI : 10.1371/journal.pone.0053302

Éditeur(s) : Gauthier-villars
Résumé : 69 new hydrothermal sites were discovered during the Naudur diving cruise. Dives were conducted between 17 degrees 5 and 18 degrees 40'5 on four segments showing marked contrast in morphology, volcanic, tectonic and hydrothermal activity. At 17 degrees 10'5, 17 degrees 25'5 and 18 degrees 37'5 the ridge has a dome shaped cross-section and is dominated by very active volcanic activity. Early widespread low temperature (<50 degrees C) diffuse hydrothermal discharge is followed by focused high temperature black smokers. At 18 degrees 15'S tectonic activity is dominant, no recent lava was observed, and only two of the 20 hydrothermal sites are active. At 18 degrees 32'5 recent lava representing a new volcanic episode partly covers the bottom of the graben. Deep hydrothermal convection is reactivated with new black smokers along the graben wall. Also, there is low temperature shimmering water from cooling lava flows. These three segments can be considered as successive volcanic/tectonic episodes typical of a fast spreading ridge. Further, the observations support a new model for the temporal evolution of episodic hydrothermal activity. Hydrothermal convection is unstable and superficial during the volcanic stage (dykes injection). At the beginning of the teaonic stage (graben formation) faults allow deep circulation and hot fluid to reach the surface. As the graben widens hydrothermal activity is less important and may cease. The heat of a new volcanic episode reactivates the deep hot water circulation along the graben faults completing a cycle.
69 nouveaux sites hydrothermaux ont été découverts entre 17'5 et 18'40'5 sur quatre segments présentant des activités volcaniques et tectoniques contrastées. A 17°10'S,17025'S et 18'37'5, la ride forme un dôme et l'activité volcanique est dominante. Les émissions hydrothermales sont d'abord diffuses et de basse température, puis focalisées et de haute température. A 18°15'S la majorité des sites hydrothermaux sont inactifs dans un graben axial dépourvu de laves récentes. A 18'32'5, des laves récentes s'épanchent au fond du graben. Les circuits hydrothermaux réactivés par le nouvel épisode volcanique se traduisent par des diffusions sur les laves récentes et par une reprise des émissions de haute température le long des murs du graben. Ces quatre segments traduisent des épisodes volcanoltectoniques successifs, caractéristiques de I'évolution temporelle des dorsales rapides. Les observations permettent de proposer un nouveau modèle de l'activité hydrothermale. Les circuits hydrothermaux sont diffus, peu structurés, instables et superficiels durant les épisodes volcaniques. Au début des épisodes tectoniques, les failles du graben drainent les fluides chauds en profondeur, puis lorsque le graben s'élargit, l'activité s'interrompt. La reprise de l'activité volcanique réamorce les circulations profondes le long des failles du graben.
Comptes Rendus de l'Academie des Sciences Serie II (1251-8069) (Gauthier-villars), 1994-12 , Vol. 319 , N. 11 , P. 1399-1406

Droits : Académie des Sciences
http://archimer.ifremer.fr/doc/00183/29455/27889.pdf
http://archimer.ifremer.fr/doc/00183/29455/

Éditeur(s) : Elsevier France-editions Scientifiques Medicales Elsevier
Résumé : The essential characteristics of the vegetation dynamics of tropical Africa remain only partially known. This study assesses the succession of vegetation-types over Central Africa during the last two glacial/interglacial cycles. Analysis of core KZai 02, which contains pollen from the Zaire River watershed (latitudes 9°N-13°S), allows the investigation of long-term patterns of plant ecosystem development and their climatic causes. Core KZai 02 (18.20 m long) was recovered from 6°24.20′S/9°54.10′E in the uppermost axial edifice of the Zaire deep sea fan. The chronology of this sedimentary archive was established using nannofossils and correlations of pollen and total organic carbon signals with the nearby core GeoB1008. The pollen record indicates that: (i) glacials (MIS 6, 4, 2) are marked by the development of afromontane (Podocarpus) forest at high altitudes when central basin lowlands were occupied by Cyperaceae marshes and savannah; (ii) during interglacials (MIS 1, 5) lowland forests were developed, marked by the successive expansion of pioneer, warm-temperate, rain forests, and mangrove indicating sea-level rise; (iii) glacial-interglacial transitions (MIS 6/5, 2/1) display similar vegetation dynamics. The strong evidence of afromontane forest and the opening of the vegetation during glacials suggest a reduced latitudinal distribution of rainfall by the strengthening of the trade wind system. West African monsoon systems were enhanced during interglacials, allowing the progressive development of lowland forests. The development of rain and pioneer forests during glacial Heinrich stadials suggests an enhancement of water availability in tropical Africa associated with these high latitude events. However, no augmentation of wind activity, described by previous studies, is evidenced by our pollen record. Similar vegetation successions during glacial/interglacial transitions suggest the diachronous and stepped intervention of CO2 (emphasizing the influence of temperature on plant ecosystems) and water availability.
Geobios (0016-6995) (Elsevier France-editions Scientifiques Medicales Elsevier), 2014-07 , Vol. 47 , N. 4 , P. 183-198

Droits : 2014 Elsevier Masson SAS. All rights reserved.
http://archimer.ifremer.fr/doc/00199/31031/29436.pdf
DOI:10.1016/j.geobios.2014.06.002
http://archimer.ifremer.fr/doc/00199/31031/

Éditeur(s) : ISTPM
Résumé : Les résultats des recherches effectuées par la "Thalassa", au cours de l'été 1971, sur le plateau de la Guyane française, montrent qu'à cette période de l'année les rendements étaient nettement insuffisants pour donner lieu à une exploitation rentable de la part des flotilles de pêche qui avaient d'ailleurs déserté ce secteur. Toutefois, la courte durée de la campagne ne permet pas d'extrapoler ces résultats à un cycle annuel complet car on connaît mal le comportement des crustacés et des poissons de cette région en fonction des variations du milieu. Les conditions hydrologiques rencontrées sont, en effet, sujettes à des fluctuations plus ou moins importantes, notamment dans la couche superficielle qui est influencée par les apports irréguliers de l'Amazone et sur les fonds de 50 à 70 m où elles sont assujetties aux mouvements plus profonds du large. De telles variations brutales sont susceptibles d'entraîner la dispersion ou la concentration de certaines espèces dans des secteurs particuliers, voire l'enfouissement des crevettes dans des terriers. Il apparaît d'ailleurs que la répartition de la faune est également liée à la nature des fonds autant qu'à la situation thermohaline du moment et l'on ne peut dire, faute d'éléments de comparaison, si les conditions observées en juillet étaient optimales ou non pour la plupart des espèces. La faible quantité de crevettes pêchées par la "Thalassa" sur le plateau continental au cours des chalutages, pourtant effectués dans les meilleures conditions, ne permet pas de définir de façon suffisamment précise les classes d'âge qui permettraient d'estimer s'il y a surexploitation ou non de ces espèces. Toutefois, les statistiques présentées par l'armement le plus important, basé à Cayenne, montre, pour l'ensemble de la région située entre le Maroni et l'Amazone, une baisse sensible du rendement journalier qui est passé de 189 kg de crevettes étêtées en 1967 à 137 kg en 1970, soit, approximativement, de 30 à 22 kg à l'heure de crevettes entières. Quant aux résultats obtenus, sur les poissons commerciaux, ils sont relativement faibles (400 kg par heure au maximum) surtout pour une région où ils ne sont pratiquement pas exploités. Ces ressources ne peuvent représenter qu'un appoint, néanmoins appréciable, pour les professionnels locaux ou même antillais qui fréquenteraient cette zone. Mais là encore, on ne connaît rien des migrations des principales espèces capturées qui peuvent être plus nombreuses à certaines époques, d'autant plus que d'autres, communes à ces régions, n'ont fait que de rares apparitions au cours des chalutages.
Science et Pêche (0036-8350) (ISTPM), 1972 , Vol. 210 , P. 1-22

Droits : Ifremer
http://archimer.ifremer.fr/doc/1972/publication-7015.pdf
http://archimer.ifremer.fr/doc/00000/7015/