Éditeur(s) : HAL CCSD Elsevier
Résumé : International audience
Hepatitis B virus (HBV) infection is a major public health problem. Recently, the human liver bile acid transporter Na+/taurocholate cotransporting polypeptide (NTCP) has been identified as an HBV specific receptor. NTCP expression is known to be strongly regulated by IL-6. This study was aimed at characterizing the effect of IL-6 on HBV entry. HBV entry was inhibited by up to 90% when cells were pretreated with IL-6 as shown by a strong inhibition of long term HBsAg secretion. This effect was confirmed by showing a severe reduction of intracellular HBV cccDNA. In parallel, we observed a 98% decrease in NTCP mRNA steady state level and an 80% reduction in NTCP-mediated taurocholate uptake. IL-6-mediated inhibition of NTCP-mediated taurocholate uptake and viral entry exhibited similar dose-dependence and kinetics while restoration of NTCP expression suppressed the inhibitory effect of IL-6. NTCP-mediated HBV entry is therefore markedly inhibited by IL-6.
ISSN: 0042-6822

hal-01134184
https://hal-univ-rennes1.archives-ouvertes.fr/hal-01134184
DOI : 10.1016/j.virol.2015.02.026
PUBMED : 25765005

Éditeur(s) : HAL CCSD
Résumé : International audience
MOTIVATION: Imaging mass spectrometry (IMS) is a maturating technique of molecular imaging. Confidence in the reproducible quality of IMS data is essential for its integration into routine use. However, the predominant method for assessing quality is visual examination, a time consuming, unstandardized and non-scalable approach. So far, the problem of assessing the quality has only been marginally addressed and existing measures do not account for the spatial information of IMS data. Importantly, no approach exists for unbiased evaluation of potential quality measures. RESULTS: We propose a novel approach for evaluating potential measures by creating a gold-standard set using collective expert judgements upon which we evaluated image-based measures. To produce a gold standard, we engaged 80 IMS experts, each to rate the relative quality between 52 pairs of ion images from MALDI-TOF IMS datasets of rat brain coronal sections. Experts' optional feedback on their expertise, the task and the survey showed that (i) they had diverse backgrounds and sufficient expertise, (ii) the task was properly understood, and (iii) the survey was comprehensible. A moderate inter-rater agreement was achieved with Krippendorff's alpha of 0.5. A gold-standard set of 634 pairs of images with accompanying ratings was constructed and showed a high agreement of 0.85. Eight families of potential measures with a range of parameters and statistical descriptors, giving 143 in total, were evaluated. Both signal-to-noise and spatial chaos-based measures performed highly with a correlation of 0.7 to 0.9 with the gold standard ratings. Moreover, we showed that a composite measure with the linear coefficients (trained on the gold standard with regularized least squares optimization and lasso) showed a strong linear correlation of 0.94 and an accuracy of 0.98 in predicting which image in a pair was of higher quality. AVAILABILITY AND IMPLEMENTATION: The anonymized data collected from the survey and the Matlab source code for data processing can be found at: https://github.com/alexandrovteam/IMS_quality. CONTACT: theodore.alexandrov@embl.de
Bioinformatics (Oxford, England)

hal-01163754
https://hal-univ-rennes1.archives-ouvertes.fr/hal-01163754
DOI : 10.1093/bioinformatics/btv266
PUBMED : 26072506

Éditeur(s) : HAL CCSD Society for the Study of Reproduction
Résumé : International audience
The endonuclease DICER that processes micro-RNAs and small interfering RNAs is essential for normal spermatogenesis and male fertility. We previously showed that the deletion of Dicer1 gene in postnatal spermatogonia in mice using Ngn3 promoter-driven Cre expression caused severe defects in the morphogenesis of haploid spermatid to mature spermatozoon, including problems in cell polarization and nuclear elongation. In this study, we further analyzed the same mouse model and revealed that absence of functional DICER in differentiating male germ cells induces disorganization of the cell-cell junctions in the seminiferous epithelium. We detected discontinuous and irregular apical ectoplasmic specializations between elongating spermatids and Sertoli cells. The defective anchoring of spermatids to Sertoli cells caused a premature release of spermatids into the lumen. Our findings may help also explain the abnormal elongation process of remaining spermatids because these junctions and the correct positioning of germ cells in the epithelium are critically important for the progression of spermiogenesis. Interestingly, cell adhesion-related genes were generally upregulated in Dicer1 knockout germ cells. Claudin 5 ( Cldn5 ) was among the most upregulated genes and we show that the polarized localization of CLAUDIN5 in the apical ectoplasmic specializations was lost in Dicer1 knockout spermatids. Our results suggest that DICER-dependent pathways control the formation and organization of cell-cell junctions in the seminiferous epithelium via the regulation of cell adhesion-related genes
ISSN: 0006-3363

hal-01274239
https://hal-univ-rennes1.archives-ouvertes.fr/hal-01274239
DOI : 10.1095/biolreprod.115.131938
PUBMED : 26510868

Éditeur(s) : HAL CCSD Springer Verlag
Résumé : International audience
We study the convergence of the Mann Iteration applied to the partial complement of a firmly nonexpansive operator with respect to a linear subspace of a Hilbert space. A new concept considered here. A regularized version is also proposed. Furthermore, to motivate this concept, some applications to robust regression procedures and location problems are proposed.
ISSN: 1862-4472

hal-00776655
https://hal.univ-antilles.fr/hal-00776655
DOI : 10.1007/s11590-009-0172-3

Éditeur(s) : HAL CCSD EDP Sciences
Résumé : International audience
We show first results of 3D simulation of sea currents in a realistic context. We use the full Navier-Stokes equations for incompressible viscous fluid. The problem is solved using a second order incremental projection method associated with the finite volume of the staggered (MAC) scheme for the spatial discretization. After validation on classical cases, it is used in a numerical simulation of the Pointe à Pitre harbour area. The use of the fictious domain method permits us to take into account the complexity of bathymetric data and allows us to work with regular meshes and thus preserves the efficiency essential for a 3D code.
ISSN: 1270-900X
Guidel, France

hal-00728894
https://hal.archives-ouvertes.fr/hal-00728894
DOI : 10.1051/proc/201235025

Éditeur(s) : HAL CCSD Springer Berlin / Heidelberg
Résumé : International audience
In distributed collaborative systems, replicated objects, shared by users, are subject to concurrency constraints. All methods [4, 13, 18, 15, 16, 19, 22] proposed to serialize concurrent operations and achieve copies convergence of replicated objects are based on the use of Operational Transformations. In this context, giving the user the ability to undo an operation has been recognized as a difficult problem [1, 2, 3, 12, 14, 20, 21]. The few general propositions to solve the problem sometimes compromise copies convergence and/or users' intention, insofar as the Operational Transformations used are unsuitable for undo. This paper has a twofold objective. Firstly, it aims to highlight two general conditions (named C3 and C4) that need to be satisfied by any transformation adapted to undo. Secondly, it presents a general undo algorithm based on the definition of a generic undo-fitted transformation, which automatically verifies these conditions. The interest of the proposed method is that the undoing of an operation obeys to the same processing as the one used for regular operations in collaborative systems such as [15,19].
12th International Conference on Cooperative Informations Systems
Agia Napa, Cyprus

hal-00684656
https://hal.archives-ouvertes.fr/hal-00684656
DOI : 10.1007/978-3-540-30468-5_12

Éditeur(s) : HAL CCSD
Résumé : International audience
In the paper the following mixed equilibrium problem in a Hilbert space is considered: find x 2 C such that F(x, x)+hG(x), x−xi 0, 8x 2 C, where C is a nonempty convex closed subset of a real Hilbert space. For its solution a version of the proximal approach is studied. The general proximal method always includes a quadratic term of the kind |x−xn|2, which provides a process of transition from a point xn to a point xn+1. The authors replace this quadratic term by its linearization. Thus, they obtain a method of the kind F(xn+1, x)+hG(xn)+ −1(h 0(xn+1)−h 0(xk)), x−xn+1i 0, 8x 2 C, where h0 is the derivative of a given function on C. It is proved that if the function F(x, y) is monotone and convex in y for any x, F(x, x) = 0, and the operator G(x) is co-coercive, then the method weakly converges in a real Hilbert space to an equilibrium solution. Note that this result will also be true if the co-coercive condition is replaced by a monotonicity property. The Tikhonov regularization method (in the absence of disturbances and calculating auxiliary solutions approximately) is justified and its weak convergence in a Hilbert space is proved. It is known that the proximal method can always be interpreted as an implicit discret approximation of some differential equation or inclusion. Therefore the authors consider a differential inclusion for the extreme mapping. Permitting "-approximation for the function F(x, y) by means of a parametric family F(x, y, ") and assuming the strong monotonicity of the operator @F(x, y, ") (it is a very strong condition), the authors prove the convergence of the trajectory of the differential inclusion to an equilibrium solution.
Ill-posed variational problems and regularization techniques

hal-00773204
https://hal.univ-antilles.fr/hal-00773204

Résumé : Selon les théories classiques du pouvoir, les relations asymétriques favorisent généralement l’acteur le plus puissant en termes de ressources. Toutefois, la partie la plus puissante au niveau de la force et des ressources ne gagne pas toujours dans les négociations. Cela dépend de plusieurs facteurs et notamment des tactiques de négociation de la partie la plus faible. Notre thèse vise justement à analyser un certain nombre de stratégies de négociations que peuvent mener les parties les plus faibles pour obtenir des gains. Afin d’illustrer nos propos, nous avons choisi l’exemple donné par un certain nombre de pays en voie de développement (PVD) pour contester le brevet sur le vivant contenu dans l’Accord sur les aspects des droits de la propriété intellectuelle (ADPIC). Notre objectif a été de comprendre les principales stratégies qu’ils ont employées pour remettre en question cette norme et la rééquilibrer. Afin de réaliser ce travail, nous nous sommes servis du concept d’hégémonie de Gramsci et de cadre emprunté à la littérature des mouvements sociaux. Cela nous a permis de poser l’hypothèse selon laquelle l’Accord sur les ADPIC constitue une norme hégémonique qui avantage essentiellement quelques pays développés et des entreprises internationales. Cependant, cette domination se cache derrière un discours universel et positif visant à susciter une forte adhésion. Ainsi, dans un tel cas de figure, les PVD ne peuvent pas espérer la remettre en question sans un changement de perception. Il faut donc que les règles perçues comme justes et immuables soient cadrées comme injustes et mutables, pour que les pays décident de les contester. Nous avons alors testé cette hypothèse à travers une analyse de contenu du discours officiel des PVD à l’Organisation mondiale du commerce. Ce travail nous a permis d’observer que le cadrage est un instrument important pour lutter contre l’hégémonie d’une norme. En effet, il offre la faculté de montrer que le brevet sur le vivant, loin de présenter que des avantages, est aussi une source de problèmes auxquels il faut apporter des solutions. Le cadre constitue aussi un bon moyen pour élaborer des propositions normatives alternatives. Toutefois, nous avons constaté que cette stratégie est insuffisante pour traduire les propositions des PVD en normes contraignantes. Afin d’obtenir certains changements normatifs, l’élaboration de coalitions s’avère particulièrement pertinente pour les PVD. De même, nous avons noté que les PVD qui demandent des changements normatifs quand la structure des opportunités politiques est ouverte ont plus de chance d’obtenir satisfaction de leurs revendications. La présente thèse nous offre ainsi un portrait général de stratégies de négociation et démontre que sous certaines conditions les parties les plus faibles peuvent obtenir certains gains dans une négociation asymétrique.
According to the classical theories of power, asymmetrical relations generally favor the most powerful actor in terms of resources. However, the most powerful in terms of strength and resources part does not always win in negotiations. This depends on several factors, including negotiation tactics of the weaker party. Our thesis aims to analyze different type of negotiation strategies that weaker parties can use to obtain some benefit. To illustrate this point, we chose the example set by a number of developing countries (DV) to challenge the patenting of life contained in the Agreement on Trade-Related Aspects of Intellectual Property Rights (TRIPS). Our goal was to understand the key strategies they have used to question this norm and rebalance it. To make this work, we used the concept of hegemony conceptualized by Gramsci and the concept of framework borrowed from the literature of social movements. This tool has allowed us to make the assumption that TRIPS is a hegemonic norm that mainly benefit to few developed countries and international companies. However, this domination is hidden by the norms thanks to universal and positive discourse which help to generate strong adhesion. Thus, in such a case, developing countries can’t expect to challenge this norm without a change of perception. It is therefore necessary that the rules perceived as fair and immutable are framed as unjust and mutable, so that countries decide to challenge it. We then tested this hypothesis through a content analysis of the DC’s official discourse at the World Trade Organization. This work has allowed us to observe that the framing is an important tool in the fight against the norm’s hegemony. Indeed, it offers the possibility to show that the patenting of life, far from presenting just a source of benefits, is also a source of problems that need solutions. The frame is also a good way to develop alternative normative propositions. However, we found that this strategy is insufficient to translate DC’s proposals into binding norms. In order to obtain changes in the agreement, building coalitions is particularly relevant for DC. Furthermore, we noted that DC which require regulatory changes when the structure of political opportunities is opened are more likely to obtain what they want. This thesis offers us a general picture of negotiations strategies and reveal that under certain conditions the weaker parties can get some gains in an asymmetrical negotiation.

http://www.theses.fr/2013AGUY0642/document

Éditeur(s) : HAL CCSD Elsevier
Résumé : International audience
Relying on the prox-regularity notion, we introduce and establish the convergence of two-step projection methods for a system of nonconvex variational inequality problems. We obtain as a particular case some known results in this field.
ISSN: 0362-546X

hal-00779983
https://hal.univ-antilles.fr/hal-00779983
DOI : 10.1016/j.na.2008.10.119

Éditeur(s) : HAL CCSD
Résumé : International audience
We show first results of 3D simulation of sea currents in a realistic context. We use the full Navier-Stokes equations for incompressible viscous fluid. The problem is solved using a second order incremental projection method associated with the finite volume of the staggered (MAC) scheme for the spatial discretization. After validation on classical cases, it is used in a numerical simulation of the Pointe à Pitre harbour area. The use of the fictious domain method permits us to take into account the complexity of bathymetric data and allows us to work with regular meshes and thus preserves the efficiency essential for a 3D code.
ESAIM : Proceedings
Guidel, France

hal-00770270
https://hal.univ-antilles.fr/hal-00770270
DOI : 10.1051/proc/201235025

Résumé : De plus en plus, le bruit est considéré comme une véritable pollution. dans le passé, les carrioles, les sabots des chevaux sur les pavés causaient déjà des nuisances aux habitants des villes. dès le xixe siècle, l'évolution de l'industrialisation entraîna une augmentation de la machinerie, qui ne fit qu'augmenter les nuisances sonores. aujourd'hui, le bruit est la cause de nombreuses plaintes. en conséquence, réduire la pollution sonore est devenu une question majeure du fait de ses effets sur la santé humaine. le bruit peut affecter le sommeil, le système cardiovasculaire, le système cérébral, particulièrement le développement cérébral des enfants. il peut causer des déficiences mentales, des problèmes de concentration et des pertes de mémoire. c'est un problème de santé publique. mais le bruit peut aussi entraîner la commission de crimes ou de suicides ! c'est aussi une question d'ordre public.en france, il existe certains dispositifs juridiques de contrôle des émissions sonores et de lutte contre les nuisances, particulièrement depuis la loi « bruit » de 1992. diverses dispositions ont été codifiées dans le code de l'environnement, les classant en fonction des différentes sources de bruit, mais aussi dans d'autres codes, notamment le code de l'urbanisme. il existe une police environnementale du bruit dont le maire et le préfet sont les deux autorités compétentes. tous ces moyens permettent de pallier les problèmes dûs au bruit à court ou à moyen terme.cependant, aujourd'hui, au regard de la préoccupation de développement durable, il convient de penser aux générations futures et de mettre en place des nouveaux moyens de régulation de la pollution sonore, à long terme. le droit de l'urbanisme apparaît comme le moyen privilégié d'atteindre ce but. le bruit pourrait être régulé par une utilisation plus rationnelle des sols… le but de ma recherche est d'analyser quel type de relation existe entre droit du bruit et droit de l'urbanisme et de mettre en évidence vers quelle forme d'urbanisme la société évolue sous l'influence combinée de l'écologie et de la question sonore, notamment à travers les objectifs posés par le grenelle de l'environnement et les lois adoptées en conséquence.
Noise is considered as a real pollution for the quality of life. Law has been requested to respond the multi-form cases of noise pollution. The matter is firstly the concern of environmental law. It is especially treated in a sectorial way. Town planning law also seizes the question, in a diffuse way, as environmental issue, or in a specific way when noise pollutions are directly caused by the use of grounds.However, under the influence of more and more pervasive environmental law, and further to the new legislation resulting from the Grenelle of the environment, town planning law underwent a deep transformation. It has been rewritten on the basis of new environmental objectives and of sustainable development. Town planning law also absorbs many other juridical sectors. Therefore it appears as a global space law and living environment law, allowing to improve the sound context.The perception of noise has changed, as well as its consideration into town planning law. Town planning law can be considered as a favorable measure to develop a more global and unified approach of the very composite legal system against noise pollution.The study of the relationship between noise and town planning highlights new manners to consider noise into space and living environment, differently from the classic approach imposed by environmental law.

http://www.theses.fr/2014AGUY0711/document

Résumé : The use of acoustical systems in various human activities in the ocean (industry, defence, science) raises the issue of their impact upon marine mammals populations. Several serious accidents linked to the use of naval sonars led the military, industrial and scientific communities to investigate the potential dangers of their own activity. This report aims at identifying the risks for the cetaceans linked to the use of acoustic and seismic systems currently used for oceanographic science. The analysis proposed here stays within the frame of bibliography synthesis and elementary modelling, and brings no new scientific elements on the topic. An overview of the status of cetacean populations is given first, with a summary of the possible acoustical threats. Risks are classified and described, insisting on the cases of massive stranding events. The present status of knowledge about auditory characteristics of cetaceans is summarised, and the definition of an objective threshold for acoustical risk is discussed ; the usually-admitted values are finally retained, despite of their evident limitations. The report presents also the emission characteristics of the various acoustic and seismic systems used in oceanographic, emphasising the ones operated by Ifremer; for the potentially harmful ones (according to the currently-used criteria) a model is proposed for the radiated acoustical intensity field, giving the extent of risk areas. This analysis concludes to the harmlessness of most systems, excepted heavy seismics and at a lesser degree low-frequency multibeam echosounders. Finally a variety of possible mitigation measures are presented and discussed; the regulations imposed by some countries are detailed. The report finally recommends both short-term measures aimed at limiting the potential risks, and longer-term actions for participating in the general effort of scientific and technological research in this field.
L'emploi des systèmes acoustiques dans les diverses activités humaines en milieu océanique (industrielles, de défense, scientifiques) pose le problème de leur impact sur les populations de mammifères marins. L'évidence de plusieurs événements graves liés à l'utilisation de sonars navals a amené les communautés militaires, industrielles et scientifiques à s'interroger sur les dangers potentiels de leurs propres activités. Le présent rapport tente de cerner les risques, pour les Cétacés, liés à l'emploi des systèmes acoustiques et sismiques couramment mis en oeuvre pour la recherche océanographique. L'analyse proposée ici reste à un niveau de synthèse bibliographique et de modélisation élémentaire, et n'offre pas d'éléments scientifiques nouveaux sur la question. On commence par donner un panorama de la situation des populations de cétacés et des agressions sonores qui les menacent. Les risques acoustiques sont répertoriés et décrits, en insistant sur les cas avérés d'échouages massifs. L'état actuel de connaissances sur les caractéristiques auditives des cétacés est résumé, et la définition d'un seuil objectif de risque acoustique est discutée ; on retient par défaut les valeurs de seuils généralement admises actuellement, en dépit de leurs limitations évidentes.

Droits : info:eu-repo/semantics/openAccess
http://archimer.ifremer.fr/doc/2007/rapport-2390.pdf
http://archimer.ifremer.fr/doc/00000/2390/

Éditeur(s) : HAL CCSD Elsevier
Résumé : International audience
BACKGROUND AND AIMS: Infection by hepatitis C virus is a worldwide health problem. An inadequate Th2 cytokine response promotes the fibrosis-cirrhosis fate. Immune-modulating molecules favoring a Th2 profile, such as HLA-G molecules of the HLA class Ib family, may play a role in chronic hepatitis. HLA-G contributes to the escape of tumors, and their involvement in viral infections has been increasingly described. The aim of this work was to study the expression of HLA-G in the liver, its cellular source and its regulation in cases of chronic C hepatitis. METHODS: HLA-G cells in blocks of liver derived from patients infected with HCV were labeled by immunohistochemistry and enumerated. Double immunofluorescence allowed the identification of the cellular source. HLA-G secretion by a human mast cell line was quantified by ELISA after various stimulations. After treatment with IFN-α real-time PCR was performed to determine the kinetics of cytokine expression profiles, followed by heat map clustering analysis. RESULTS: The number of HLA-G + cells was significantly associated with the area of fibrosis. For the first time, we identify the HLA-G+ cells as being mast cells. HLA-G secretion was significantly induced in human mast cells stimulated by IL-10 or interferons of class I. The transcriptome of the secretome of this cell line stimulated by IFN-α revealed that i) the HLA-G gene is upregulated late, ii) T lymphocytes and NK cells are recruited. CONCLUSIONS: These findings suggest an autocrine loop in the genesis of HCV liver fibrosis, based on mast cells expressing HLA-G.
ISSN: 0168-8278

hal-00874106
https://hal.archives-ouvertes.fr/hal-00874106
https://hal.archives-ouvertes.fr/hal-00874106/document
https://hal.archives-ouvertes.fr/hal-00874106/file/Amiot-Expression-HLA-G-revised.pdf
DOI : 10.1016/j.jhep.2013.09.006

Éditeur(s) : HAL CCSD Elsevier
Résumé : International audience
The strikingly complex structural organization of the mammalian testis in vivo creates particular difficulties for studies of its organization, function and regulation. These difficulties are particularly pronounced for investigations of the molecular communication networks within the seminiferous tubules that govern spermatogenesis. The use of classical molecular and cell biology approaches to unravel this complexity has proved problematic, due to difficulties in maintaining differentiated germ cells in vitro, in particular. The lack of a suitable testing ground has led to a greater reliance on high-quality proteomic and genomic analyses as a prelude to the in vitro antx1d in vivo testing of hypotheses. In this study, we highlight the options currently available for research, as used in our laboratory, in which proteomic and integrative genomic strategies are applied to the study of spermatogenesis in mammals. We will comment on results providing insight into the molecular mechanisms underlying normal and pathological spermatogenesis and new perspectives for the treatment of male infertility in humans. Finally, we will discuss the relevance of our strategies and the unexpected potential and perspectives they offer to teams involved in the study of male reproduction, within the framework of the Human Proteome Project. SIGNIFICANCE: Integrative genomics is becoming a powerful strategy for discovering the biological significance hidden in proteomic datasets. This work introduces some of the integrative genomic concepts and works used by our team to gain new insight into mammalian spermatogenesis, a remarkably sophisticated process. We demonstrate the relevance of these integrative approaches to understand the cellular cross talks established between the somatic Sertoli cells and the germ cell lineage, within the seminiferous epithelium. Our work also contributes to new knowledge on the pathophysiology of testicular function, with promising clinical applications. This article is part of a Special Issue entitled: 20years of Proteomics in memory of Viatliano Pallini. Guest Editors: Luca Bini, Juan J. Calvete, Natacha Turck, Denis Hochstrasser and Jean-Charles Sanchez.
ISSN: 1874-3919

hal-01063931
https://hal.archives-ouvertes.fr/hal-01063931
DOI : 10.1016/j.jprot.2014.04.013
PUBMED : 24751586

Résumé : À la Martinique au XVIIIe siècle, les « libres de couleur », qu’ils soient nés libres ou affranchis, noirs ou métis, forment une catégorie juridique distincte des Blancs et des esclaves. L’étude comparée, avec les territoires espagnols ou anglais, montre qu’aucune réglementation légale n’a officialisé un passage de la catégorie Libre de couleur à celle de Blanc dans les colonies françaises de la fin du XVIIe siècle à la Révolution française. Aussi, cette thèse se propose de montrer les processus officieux qui ont permis à certaines personnes – les « assimilés » – de franchir la barrière de couleur. Une réflexion a été menée sur le préjugé de couleur, système raciste dont l’idéologie, soutenue par la réglementation locale, a légitimé la construction collective d’un ordre public et social. Puis, on a examiné l’élaboration de la barrière de couleur. Les libres de couleur ont été les premiers à faire les frais de l’imperméabilisation de la ligne de démarcation et des problèmes posés par la pureté de sang ; mais les Blancs mésalliés, dans le cadre d’unions interraciales, et les Amérindiens ont aussi été visés. Enfin nous avons réfléchi à ce qui faisait la blancheur et aux stratégies adoptées pour réussir ce changement de statut. Le notariat et les 33 000 actes des registres paroissiaux traités ont donné lieu à la reconstitution de généalogies pour examiner des individus et des familles sur plusieurs générations ; on a ainsi observé l’importance du phénotype, le blanchiment, la légitimité des relations, les conjoints privilégiés, le choix des réseaux, les niveaux de fortune et l’usage de l’espace.
In Martinique in the eighteenth century, the "free people of color", both those free by birth and freedmen, black or mixed race, form a legal category, which was distinct from those of whites and slaves. Comparative studies with Spanish or English territories show that no legal regulation formalized a shift - from the category of free colored people to that of White - in the French colonies between the late seventeenth century and the French Revolution. Also, this thesis proposes to show the informal process that enabled some people - the "assimilated" - to cross the color barrier. I analysed the color prejudice, a racist system, whose ideology, supported by local regulations, legitimized the collective construction of a public and social order. In a second step, I examined the development of the color bar. The free colored people were the first to bear the brunt of the impermeability of the demarcation line and of the problems posed by the purity of blood; but some whites, through interracial unions, and Native Americans have also been targeted. Finally I thought about what made the whiteness, and the strategies to achieve whiteness, change in status. Notarial acts and 33,000 acts of parish registers treaties led to the reconstitution of genealogies, in order to examine individuals and families over generations; I observed the importance of the phenotype, whitening, legitimacy relations, privileged partners, choice of networks, wealth levels and the use of space.

http://www.theses.fr/2015AGUY0847/document

Résumé : La dengue constitue actuellement l'arbovirose la plus répandue sur la planète avec 40% de la population mondiale exposée, soit environ 2,5 milliards de personnes. La maladie est endémique dans tous les continents excepté l'Europe même si les premiers cas de transmission autochtone ont été observés en France métropolitaine et en Croatie en 2010. L'augmentation régulière de son incidence fait actuellement de la dengue un problème de santé publique majeur dans les pays tropicaux et une menace pour d'autres pays du monde. En raison de son mode de transmission, la dengue constitue une maladie étroitement liée à l'environnement. L'urbanisation constitue un facteur important dans l'émergence ou la réémergence d'épidémies de dengue dans les pays tropicaux. Cette étude menée a pour objet d'analyser les liens existant entre le domaine de la santé (situation sanitaire et épidémiologique de la dengue) et de celui de l'environnement (évolution de l'urbanisation) en Guadeloupe continentale entre 2000 et 2008.Pour ce faire, les cas suspects et confirmés de dengue ont été recueillis. L'incidence pour ces deux types de cas a été calculée puis représentée à deux échelles administratives différentes: communes et sections. Les facteurs environnementaux comprenant les variables physiques et socio-démographiques ont été pris en compte pour la définition, l'identification et l'analyse de l'évolution des contextes d'habitation durant la période étudiée. Deux méthodes ont été utilisées: la classification «Raster» consistant en une analyse régulière basée sur des mailles de taille identique et la classification « Vecteur» s'appuyant sur les limites administratives des sections. Ces classifications ont permis de décrire la répartition des différents contextes d'habitation sur le territoire de la Guadeloupe et d'obtenir des indicateurs qui ont été analysés en relation avec les données sanitaires. Il a été mis en évidence que la situation épidémiologique de la dengue dans le département s'est significativement aggravée au cours de la période étudiée. Des épidémies majeures sont apparues en 2001, 2005/2006 et 2007. Le nord Basse-Terre, la région Pointoise ainsi que le sud-est de la Grande-Terre, de la ville du Gosier à Saint-François le long de la côte, sont les régions les plus concernées par l'augmentation d'incidence de la maladie. Concernant les contextes d'habitation, huit contextes distincts ont été identifiés: touristique, naturel, intermédiaire, agricole, résidentiel, rural, périurbain et urbain. Le contexte intermédiaire correspond à des régions de transition entre les autres contextes d'habitation. La différence entre les contextes agricoles et ruraux réside dans la prédominance de surface agricole pour le premier alors que le contexte rural est caractérisé par les espaces cultivés habités. L'évolution surfacique de ces contextes au cours du temps a été analysée en relation avec les incidences. Les résultats montrent que les contextes d'habitation sont statistiquement.associés à l'incidence de la dengue en Guadeloupe. En particulier, les contextes touristiques, résidentiels et ruraux possèdent une association positive élevée avec l'incidence de la dengue.
At present, dengue fever has become the arbovirose the most wide-spread worldwide with 40 % ofthe exposed population, 2,5 billion people. The disease is endemie in all the continents excepted Europe even though the fust cases ofautochthonoustransmissionwere observedinmetropolitanFranceandCroatiain2010.Because oftherecentincrease of its incidence, dengue fever is now a major public health problem in the tropical countries and a threat for the other countries in the worldwide. Due to its mode oftransmission, the disease is closely linked to the environment. Urbanization is an important factor ofthe emergence or the re-emergence of dengue in the tropical countries. This study aims to analyze the links between health and environment domains, which correspond to the epidemiological situation of the dengue and the evolution ofthe urbanization in Guadeloupe.Information about suspect and confirmed cases of dengue were collected. The incidence of suspect and confirmed cases has been estimated, then represented at two different administrative boundaries: communes and sections. Environmental factors including the physical and socio-demographic variables were taken into account to the definition, the identification and the analyze ofevolution of the housing patterns during the studied period. Two methods were used: Il Raster" consisting of a regular analysis based on squares units and "Vector" based on the administrative limits of sections. These classifications allowed to describe the distribution ofthe different housing patterns ofGuadeloupe and to estimate indicators for the analyzing of sanitary data. The results highlight a worsening ofepidemiological situation ofthe dengue over the time in the department. Major outbreaks occurred in 2001,2005/2006 and 2007. The north Basse-Terre, the Pointe-à-Pitre urban area as weIl as the whole coast (frOID the city of Gosier to Saint-François) ofthe southeast ofGrande-Terre were the most affected regions by the increase ofincidence of dengue. Concerning the housing classes, eight different patterns were identified: tourist, natural, intermediate, agricultural, residential, rural, suburban and urban. The intermediate class corresponds to buffer regions between the other patterns. The difference between the agricultural and rural contexts lies in the predominance ofagricultural surface for the first one while the rural context is characterized by the inhabited farmland. The evolution over time of spatial extend ofthese patterns was described in relation with incidence. Results showed that the housing patterns are statically associated with the incidence ofdengue in Guadeloupe. In particular, the touristic, residential and rural patterns have a high positive association with dengue incidence.

http://www.theses.fr/2012AGUY0565/document