Éditeur(s) : Wiley-blackwell Publishing, Inc
Résumé : Vibrio splendidus, strain LGP32, is an oyster pathogen associated with the summer mortalities affecting the production of Crassostrea gigas oysters worldwide. Vibrio splendidus LGP32 was shown to resist to up to 10 mu M Cg-Def defensin and Cg-BPI bactericidal permeability increasing protein, two antimicrobial peptides/proteins (AMPs) involved in C. gigas immunity. The resistance to both oyster Cg-Def and Cg-BPI and standard AMPs (polymyxin B, protegrin, human BPI) was dependent on the ompU gene. Indeed, upon ompU inactivation, minimal bactericidal concentrations decreased by up to fourfold. AMP resistance was restored upon ectopic expression of ompU. The susceptibility of bacterial membranes to AMP-induced damages was independent of the ompU-mediated AMP resistance. Besides its role in AMP resistance, ompU proved to be essential for the adherence of V. splendidus LGP32 to fibronectin. Interestingly, in vivo, ompU was identified as a major determinant of V. spiendidus pathogenicity in oyster experimental infections. Indeed, the V. splendidus-induced oyster mortalities dropped from 56% to 11% upon ompU mutation (Kaplan Meier survival curves, P < 0.01). Moreover, in co-infection assays, the ompU mutant was out competed by the wild-type strain with competitive indexes in the range of 0.1-0.2. From this study, ompU is required for virulence of V. splendidus. Contributing to AMP resistance, conferring adhesive properties to V. splendidus, and being essential for in vivo fitness, the OmpU porin appears as an essential effector of the C. gigas/V. splendidus interaction.
Environmental Microbiology (1462-2912) (Wiley-blackwell Publishing, Inc), 2010-04 , Vol. 12 , N. 4 , P. 951-963

Droits : 2010 Society for Applied Microbiology and Blackwell Publishing Ltd
http://archimer.ifremer.fr/doc/00003/11421/8044.pdf
DOI:10.1111/j.1462-2920.2009.02138.x
http://archimer.ifremer.fr/doc/00003/11421/

Éditeur(s) : Springer
Résumé : We investigated the habitat use in green turtles exploiting a 13-ha multispecific seagrass meadow at Mayotte Island, south-western Indian Ocean. A phytoecological survey shows the occurrence of eight seagrass species, dominated by Halodule uninervis and Syringodium isoetifolium, distributed according to four distinct seagrass communities along the depth gradient. Direct underwater censuses show that green turtles occurred all over the meadow. Yet when community relative surface area was taken into account green turtles preferentially frequented the most seaward, biomass-richer S. isoetifolium-dominated community, suggesting that green turtles compensate for their intrinsically nutrient-poor herbivorous diet. Additionally, smaller (80 cm standard curved carapace length, SCCL) individuals also preferentially occurred in the most shoreward H. univervis-dominated community where no larger ([80 cm SCCL) individuals were sighted, suggesting habitat use is indicative of diet selection and may reflect size-specific food requirements and physiology.
Marine Biology (0025-3162) (Springer), 2010-12 , Vol. 157 , N. 12 , P. 2581-2590

Droits : 2010 Springer
http://archimer.ifremer.fr/doc/00018/12918/10201.pdf
DOI:10.1007/s00227-010-1520-7
http://archimer.ifremer.fr/doc/00018/12918/

Éditeur(s) : HAL CCSD
Résumé : The Earth's magnetic field shows a large range of temporal variations from the year to several million years. I studied during my PhD thesis several aspects of these fluctuations, from recent secular variations to reversals. The first part of my manuscript deals with archeomagnetism, a discipline that allows to track the temporal variations of the Earth's magnetic field through millennia, mainly from archeological materials. I studied two sets of samples , one from Greece (Neolithic period 6800-3200 B.C.) and the other from Mexico (Palenque, Maya Classic period 320-840 A.D.), to acquire new archeointensity data in order to better constrain the secular variation of the geomagnetic field. By comparing my data with those available in the literature and with the various global and regional models, I showed that the secular variations during the Neolithic in Greece and during the first millennium in Central America are poorly defined. My data suggest that local components, not described by global models, may exist in these regions. They reinforce the importance of developing specific regional models. As a consequence, the acquisition of new high quality data is of main importance. The second part presents the description of a geomagnetic field reversal. This work was based on two points : first by studying transitional directions to provide new constraints on the possible preferred longitudinal paths of virtual geomagnetic poles (VGPs) and second by checking transitional paleointensities obtained on a volcanic sequence in Akaroa volcano (New Zealand). Indeed the transitional field intensity is significantly higher than the field intensity before and after the reversal. We re-sampled this sequence, and the directional results show a complex sequence of N-T-R-T-N-T-R polarity. The transitional VGPs obtained are clustered in two longitudinal bands through Australia and America. This observation seems to reinforce the assumption of a core-mantle interaction over several million years. Following a rock magnetic study, I selected samples that could provide a value for the Thellier and multispecimen paleointensity methods. The obtained paleointensity are relatively low (about 20 μT) during the polarity change and strong at the end of the sequence. Based both on the field strength values and on the radiochronological ages, showing that the sequence was erupted in a very short time, I suggest that only the C4AR.1n-C4Ar.1r reversal was recorded in this sequence. In this assumption, the reversal shows a complex path comparable to other reversals recorded in the northern hemisphere (for example the Steens Mountain ), including a rebound before stabilizing. Finally the last part is devoted to the development of a new methodology and a new apparatus to determine absolute paleointensity. Following the low success rate of paleointensity experiments from the previous study, I decided to test the multispecimen protocol, which can be applied to samples yielding a predominant multidomaine behavior. The main technical drawback of this method lies in the application of the laboratory field along the natural remanent magnetization, a difficult task to perform accurately in standard paleointensity ovens. Thus, we decided to adapt sample holders from our standard oven in order to allow the sample orientation in space and to develop an ultra-fast heating oven prototype particularly well-suited for this method, allowing to apply the laboratory field in the 3 dimensions. I checked the different multispecimen protocols on historical lavas from Reunion and Etna volcano, yielding very different magnetic mineralogies. For all flows, I obtained paleointensities very close from the expected values, regardless from the magnetic mineralogy, revealing the feasibility of our apparatus and the promising interest of the method. The application of various corrections on the statistical estimation of the intensity value or on the cooling rate had no significant impact on my results.
La première partie de ma thèse porte sur l'archéomagnétisme, discipline qui permet de retracer l'évolution temporelle du champ magnétique terrestre au travers des derniers millénaires, principalement à partir des matériaux archéologiques. J'ai étudié deux jeux d'échantillons provenant pour l'un de la Grèce (période néolithique, 6800-3200 avant J.C.) et pour l'autre du Mexique (Palenque, période Maya Classique 320-840 après J.C.), dans le but d'acquérir de nouvelles données d'archéointensité et de mieux contraindre la variation séculaire du champ magnétique terrestre. La comparaison de mes données avec celles disponibles dans la bibliographie et avec les différents modèles globaux et régionaux a mis en évidence que les variations séculaires au Néolithique en Grèce et au cours du premier millénaire en Amérique Centrale sont très mal définies. Mes données suggèrent que des composantes locales, non prisent en compte dans les modèles globaux peuvent exister au niveau de ces régions. Elles renforcent l'intérêt de développer des modèles régionaux précis. Pour ce faire, l'acquisition de nouvelles données de haute qualitéest un élément majeur. La deuxième partie traite de la description d'un renversement du champ magnétique terrestre. Cette étude a été basée sur deux points précis : étudier les directions transitionnelles afin d'apporter de nouvelles contraintes sur le possible confinement longitudinal des pôles géomagnétiques virtuels (PGV) d'une part, et d'autre part vérifier des paléointensités transitionnelles obtenues sur la séquence volcanique d'Akaroa (Nouvelle Zélande), dont l'intensité est significativement supérieure à celles des intensités avant et après le renversement. Nous avons ré-échantillonné cette séquence, et l'évolution directionnelle obtenue pour ce renversement est une succession complexe de polarités N-T-R-T-N-T-R. Les PGV obtenus semblent se regrouper sous deux bandes longitudinales sous l'Australie et l'Amérique, ce qui renforce l'hypothèse d'une interaction entre le manteau et le noyau sur plusieurs millions d'années. Suite à une étude de minéralogie magnétique, j'ai sélectionné les échantillons susceptibles de fournir une valeur de paléointensité par les méthodes de Thellier et du multispecimen. Les paléointensités obtenues sont relativement faibles (environ 20 μT) au cours du changement de polarité et forte à la fin de la séquence. Mon interprétation, basée à la fois sur les valeurs de l'intensité du champ et sur les données radiochronologiques montrant que la séquence s'est mise en place très rapidement, est de considérer que seul le renversement C4Ar.1n-C4Ar.1r a été enregistré dans cette séquence. Dans cette hypothèse, le renversement montre un cheminement complexe comparable à d'autres renversements enregistrés dans l'hémisphère nord (comme celui de la Steens Mountain par exemple), incluant un phénomène de rebond avant de se stabiliser. La troisième partie de ma thèse est consacrée au développement d'une nouvelle méthodologie et d'un nouvel appareillage pour déterminer des paléointensités. Le faible taux de réussite des expériences de paléointensité de l'étude précédente m'a poussé à m'intéresser au protocole multispecimen, qui peut s'appliquer aux échantillons possédant un comportement polydomaine. L'inconvénient technique majeur de cette méthode réside dans l'application du champ le long de l'aimantation naturelle, difficile à réaliser avec précision dans les fours standards. Pour ce faire, nous avons décidé de développer des porte-échantillons permettant d'orienter les échantillons dans l'espace pour le four standard et un prototype de four à chauffage ultra-rapide particulièrement bien adapté avec la possibilité d'orienter le champ appliqué dans les 3 dimensions. J'ai testé les différents protocoles de la méthode sur des laves historiques de la Réunion et de l'Etna, possédant des minéralogies très différentes. Pour l'ensemble des coulées étudiées, j'ai obtenu des paléointensités très proches des valeurs attendues, et ce indépendamment de la minéralogie, révélant ainsi le faisabilité de notre appareillage et le côté prometteur de la méthode. L'application de diverses corrections sur la statistique d'estimation de la valeur de l'intensité ou sur le taux de refroidissement n'ont eu aucun impact notable sur mes résultats.
https://tel.archives-ouvertes.fr/tel-00719380

tel-00719380
https://tel.archives-ouvertes.fr/tel-00719380
https://tel.archives-ouvertes.fr/tel-00719380/document
https://tel.archives-ouvertes.fr/tel-00719380/file/thesefanjat2012.pdf

Éditeur(s) : HAL CCSD Springer Verlag
Résumé : International audience
Glioblastoma (GB) is the most frequent and aggressive type of primary brain tumor. Recurrences are mostly located at the margin of the resection cavity in the peritumoral brain zone (PBZ). Although it is widely believed that infiltrative tumor cells in this zone are responsible for GB recurrence, few studies have examined this zone. In this study, we analyzed PBZ left after surgery with a variety of techniques including radiology, histopathology, flow cytometry, genomic, transcriptomic, proteomic, and primary cell cultures. The resulting PBZ profiles were compared with those of the GB tumor zone and normal brain samples to identify characteristics specific to the PBZ. We found that tumor cell infiltration detected by standard histological analysis was present in almost one third of PBZ taken from an area that was considered normal both on standard MRI and by the neurosurgeon under an operating microscope. The panel of techniques used in this study show that the PBZ, similar to the tumor zone itself, is characterized by substantial inter-patient heterogeneity, which makes it difficult to identify representative markers. Nevertheless, we identified specific alterations in the PBZ such as the presence of selected tumor clones and stromal cells with tumorigenic and angiogenic properties. The study of GB-PBZ is a growing field of interest and this region needs to be characterized further. This will facilitate the development of new, targeted therapies for patients with GB and the development of approaches to refine the per-operative evaluation of the PBZ to optimize the surgical resection of the tumor.
ISSN: 0167-594X

hal-01117083
https://hal-univ-rennes1.archives-ouvertes.fr/hal-01117083
https://hal-univ-rennes1.archives-ouvertes.fr/hal-01117083/document
https://hal-univ-rennes1.archives-ouvertes.fr/hal-01117083/file/Characterizing%20the%20Peritumoral%20Brain%20Zone_accepted.pdf
DOI : 10.1007/s11060-014-1695-8

Éditeur(s) : HAL CCSD Elsevier
Résumé : Reliable measurements of the intensity of the palaeomagnetic field are notoriously difficult to obtain. The approach generally taken is to produce multiple estimates per rock unit and assume that those which meet certain minimum standards of technical quality will combine to produce an accurate mean. However, here, using results produced from a package of 20th century basaltic lava flows from Mount Etna in Sicily, we demonstrate that this approach can fail. In this case, the application of typical sets of selection criteria actually introduces bias into the mean determination that we measure. We demonstrate that this is caused by two types of non-ideal behaviour acting in combination. The first is a result of the multidomain grains that the samples contain and the second is caused by differences in the natural and laboratory cooling rates. We discuss means of avoiding these sources of error in future palaeointensity studies performed on ancient rocks. We also develop a new, more general reliability criterion which is effective here and which we argue should be applied wherever possible in future palaeointensity studies in conjunction with standard criteria. It requires two distinct types of materials which both produce some good-quality palaeointensity measurements and uses their range of overlap to constrain the true palaeointensity. Both the application of this criterion and general reliability considerations require that future palaeointensity studies should measure many samples per cooling unit and that these should be as diverse in terms of their rock magnetic properties and cooling histories as possible.
ISSN: 0012-821X

hal-00407943
https://hal.archives-ouvertes.fr/hal-00407943
DOI : 10.1016/j.epsl.2007.03.017

Éditeur(s) : HAL CCSD Public Library of Science
Résumé : International audience
The use of recombinant antigens has been shown to improve both the sensitivity and the standardization of the serological diagnosis of Farmer's lung disease (FLD). The aim of this study was to complete the panel of recombinant antigens available for FLD serodiagnosis with antigens of Lichtheimia corymbifera, known to be involved in FLD. L. corymbifera proteins were thus separated by 2D electrophoresis and subjected to western blotting with sera from 7 patients with FLD and 9 healthy exposed controls (HEC). FLD-associated immunoreactive proteins were identified by mass spectrometry based on a protein database specifically created for this study and subsequently produced as recombinant antigens. The ability of recombinant antigens to discriminate patients with FLD from controls was assessed by ELISA performed with sera from FLD patients (n = 41) and controls (n = 43) recruited from five university hospital pneumology departments of France and Switzerland. Forty-one FLD-associated immunoreactive proteins from L. corymbifera were identified. Six of them were produced as recombinant antigens. With a sensitivity and specificity of 81.4 and 77.3% respectively, dihydrolipoyl dehydrogenase was the most effective antigen for discriminating FLD patients from HEC. ELISA performed with the putative proteasome subunit alpha type as an antigen was especially specific (88.6%) and could thus be used for FLD confirmation. The production of recombinant antigens from L. corymbifera represents an additional step towards the development of a standardized ELISA kit for FLD diagnosis. © 2016 Rognon et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
ISSN: 1932-6203

hal-01367148
https://hal-univ-rennes1.archives-ouvertes.fr/hal-01367148
DOI : 10.1371/journal.pone.0160888

Éditeur(s) : HAL CCSD Springer Verlag
Résumé : In this paper, we investigate the impact of ambient temperature changes on the gravity reading of spring-based relative gravimeters. Controlled heating experiments using two Scintrex CG5 gravimeters allowed us to determine a linear correlation (R2> 0.9) between ambient temperature and gravity variations. The relation is stable and constant for the two CG5 we used: −5 nm/s2/∘C. A linear relation is also seen between gravity and residual sensor temperature variations (R2> 0.75), but contrary to ambient temperature, this relation is neither constant over time nor similar between the two instruments. The linear correction of ambient temperature on the controlled heating time series reduced the standard deviation at least by a factor of 2, to less than 10 nm/s2. The laboratory results allowed for reprocessing the data gathered on a field survey that originally aimed to characterize local hydrological heterogeneities on a karstic area. The correction of two years of monthly CG5 measurements from ambient temperature variations halved the standard deviation (from 62 to 32 nm/s2) and led us to a better hydrological interpretation. Although the origin of this effect is uncertain, we suggest that an imperfect control of the sensor temperature may be involved, as well as a change of the properties of an electronic component.
ISSN: 0949-7714

hal-01526952
https://hal.archives-ouvertes.fr/hal-01526952
DOI : 10.1007/s00190-016-0961-2

Résumé : This report synthesises the knowledge on the biogeochemical cycle, bioconcentration processes and ecotoxicity of lead in the marine environment. Man induced changes on the lead cycle, especially along the French coasts are reviewed. Concentration levels, mechanisms and fluxes between geochemical reservoirs are assessed. Emphasis is given on sublethal effects and toxicity mechanisms. Quality standards used in European Community countries are listed. Conclusions and recommendations for environmental management and research are given.
Le présent document constitue une synthèse des connaissances sur le cycle biogéochimique, la bioconcentration et l'écotoxicité du plomb en milieu marin. Il fait état des perturbations du cycle du plomb par l'action de l'homme, en particulier dans le milieu littoral français. Les niveaux de concentration et les quantités échangées entre les réservoirs géochimiques sont évalués. L'accent est mis sur les effets sublétaux et les mécanismes de toxicité vis-à-vis du biotope marin. Les réglementations relatives en milieu marin actuellement en vigueur dans les pays de la Communauté européenne sont répertoriées. Avec les conclusions quelques recommandations en matière de gestion et de recherche sont faites.

Droits : info:eu-repo/semantics/openAccess
http://archimer.ifremer.fr/doc/1993/rapport-1449.pdf
http://archimer.ifremer.fr/doc/00000/1449/

Éditeur(s) : HAL CCSD Lippincott, Williams & Wilkins
Résumé : International audience
Objective Large-scale prospective studies do not support an association between neuroticism and extroversion with cancer incidence. However, research on other personality constructs is inconclusive. This longitudinal study examined the associations between four personality measures, Type 1, "suppressed emotional expression"; Type 5, "rational/antiemotional"; hostility; and Type A with cancer incidence. Methods Personality measures were available for 13,768 members in the GAZEL cohort study (baseline assessment in 1993). Follow-up for diagnoses of primary cancers was obtained from January 1, 1994 to December 31, 2009. Associations between personality and cancer incidence were evaluated using Cox proportional hazards analyses and adjusted for potential confounders. Results During a median follow-up of 16.0 years (range, 9 days-16 years), 1139 participants were diagnosed as having a primary cancer. The mean duration between baseline and cancer diagnosis was 9.3 years. Type 1 personality was associated with a decreased risk of breast cancer (hazard ratio per standard deviation = 0.81, 95% confidence interval = 0.68-0.97, p = .02). Type 5 personality was not associated with prostate, breast, colorectal, or smoking-related cancers, but was associated with other cancers (hazard ratio per standard deviation = 1.17, 95% confidence interval = 1.04-1.31, p = .01). Hostility was associated with an increased risk of smoking-related cancers, which was explained by smoking habits, and Type A was not associated with any of the cancer endpoints. Conclusions Several personality measures were prospectively associated with the incidence of selected cancers. These links may warrant further epidemiological studies and investigations about potential biobehavioral mechanisms.
ISSN: 0033-3174

inserm-00813178
http://www.hal.inserm.fr/inserm-00813178
http://www.hal.inserm.fr/inserm-00813178/document
http://www.hal.inserm.fr/inserm-00813178/file/PersonalityCancer_Lemogne_Psychosom_Med_2013.pdf
http://www.hal.inserm.fr/inserm-00813178/file/inserm-00813178_edited.pdf

Éditeur(s) : HAL CCSD Elsevier
Résumé : International audience
People are exposed to multiple pollutants, especially indoors, in particular through ingestion of indoor settled dust. In the perspective of a cumulative risk assessment, a multi-residue analytical method based on pressurized liquid extraction (PLE) and gas chromatography/tandem mass spectrometry (GC/MS/MS) was developed for the simultaneous analysis in indoor dust of several classes of semi-volatile organic compounds (SVOCs) of health concern, from trace to highly concentrated compounds, including musk fragrances, organochlorines (OCs), organophosphates (OPs), polycyclic aromatic hydrocarbons (PAHs), polybromodiphenylethers (PBDEs), polychlorobiphenyls (PCBs), phthalates and pyrethroids. The method was validated in terms of limits of quantification (LOQ), and accuracy and precision via spiking experiments on an inert material (Celite(®) 545) and replicate analysis of the standard reference material SRM 2585 supplied by the National Institute of Standards and Technology (NIST). Method LOQs for 200 mg samples of sieved dust were 26 ng g(-1) for PCBs and some OCs, 65 ng g(-1) for musks, OPs, PAHs, PBDEs, pyrethroids, other OCs and some phthalates, 132 ng g(-1) for butylbenzylphthalate (BBP), 197 ng g(-1) for tributylphosphate and 1579 ng g(-1) for other phthalates. Quadratic calibration curves were established for each compound by analyzing at least five calibration solutions and exhibited determination coefficients higher than 0.999. The method was successfully applied to the SRM 2585 and seven real indoor dust samples. The results obtained on SRM 2585 demonstrate both excellent reproducibility and agreement with the indicative, reference or certified values and provide, for the first time, indicative concentrations for chlorpyrifos, diazinon, diisononylphthalate (DiNP) and tetramethrin. The results obtained on real dust samples illustrate the ability of the proposed method to quantify a wide range of SVOCs in a single analysis, making it appropriate for environmental monitoring programs or large-scale studies with a large number of samples.
ISSN: 0021-9673

hal-00994493
https://hal-univ-rennes1.archives-ouvertes.fr/hal-00994493
DOI : 10.1016/j.chroma.2014.02.004
PUBMED : 24598454

Éditeur(s) : Université des Antilles Études caribéennes
Résumé : La dernière édition de l’enquête Budget de Famille (2010-2011) de l’Insee comporte un suréchantillonnage de logements dans les départements d’outre-mer (Guadeloupe, Martinique, Guyane, Réunion et Mayotte devenue département français en 2011). Ces données permettent de dresser un panorama de la consommation des ménages résidant sur ces territoires. Des disparités peuvent alors être mises en évidence avec la France métropolitaine. En neutralisant des effets de structure des deux populations, il ressort que les ménages ultra-marins (à l’exception des Antilles) ont des dépenses de consommation plus faibles que les ménages métropolitains notamment pour les postes de l’alimentation, des loisirs et de la santé. Ils sont également un peu moins bien équipés en biens durables (véhicule, lave-linge, lave-vaisselle…). En revanche, quand on les interroge sur leur niveau de vie, les ménages ultra-marins ont — toutes choses égales par ailleurs — une perception plus positive de celui-ci que les métropolitains. Le département de Mayotte se singularise à la fois par les caractéristiques sociodémographiques de sa population, mais aussi par le faible niveau de consommation et d’équipement de ses ménages, corollaires d’un niveau de vie bien plus faible que dans les autres départements ultra-marins.
The last INSEE Family Budget Survey (2010–2011) oversamples housings in overseas departments (Guadeloupe, Martinique, Guyana, Réunion and Mayotte). Those data allow us to draw up an overview of household consumption on those territories. Disparities can be highlighted with metropolitan France. Neutralizing structural heterogeneity of populations, it emerges that ultramarine households (with the exception of Caribbean France) have lower spending than metropolitan households, notably for food, leisure and health. They are also less equipped with durable goods (vehicle, washing machine, dishwasher…). When they are questioned about their standard of living, ultramarine households have – ceteris paribus – a more positive perception than metropolitan households. Mayotte stands out both by socio-demographic characteristics of its population but also by the low level of consumption and equipment of its households. This is the result of very low standard of living of the local population.

Droits : info:eu-repo/semantics/openAccess
urn:doi:10.4000/etudescaribeennes.11323
http://journals.openedition.org/etudescaribeennes/11323

Éditeur(s) : HAL CCSD Springer Verlag
Résumé : International audience
PURPOSE: Dairy farming is a risk factor for the development of chronic obstructive pulmonary disease (COPD). We assessed the prevalence of farming-induced COPD (FI-COPD) using a new screening device, and we analyzed symptoms and occupational risk factors. METHODS: We performed on-site screening study of bronchial obstruction using an electronic mini-spirometer (EMS) on an entire population of dairy farmers (n = 147) from two villages in Brittany, France. Suspected bronchial obstruction (FEV1/FEV6 <0.8) was confirmed with standardized lung function tests (FEV1/FVC ≤0.7). We assessed past medical histories, respiratory symptoms, and occupational tasks of subjects with bronchial obstruction; asthmatics were defined as atopic and/or reversible; smoking-induced COPD patients were defined as non-reversible, non-atopic with smoking histories (≥5 PY); and FI-COPD patients were defined as non-reversible, non-atopic, and non-smokers. RESULTS: Using the EMS, 30.6% (n = 45) of dairy farmers were suspected of bronchial obstruction and underwent standardized spirometry. The FEV1/FEV6 ratio and FEV1/FVC ratio were in good agreement (r² = 0.66, P < 0.0001). The prevalence of confirmed bronchial obstruction was 9.5% (n = 14), which included 4 asthmatics, 3 smoking-induced COPD subjects, and 7 FI-COPD subjects. All the COPD patients were GOLD Stage II, and none were aware of their respiratory disease. Foddering duration was significantly higher in FI-COPD subjects compared with non-obstructive subjects, with 44 versus 17 min/day, respectively. CONCLUSIONS: The EMS was a convenient mean of screening for bronchial obstruction, especially in on-site settings, and allowed us to diagnose FI-COPD in a non-spontaneously complaining dairy farmer population. Foddering was considered a significant risk factor.
ISSN: 0340-0131

hal-00872929
https://hal.archives-ouvertes.fr/hal-00872929
DOI : 10.1007/s00420-011-0708-6
PUBMED : 21986906

Éditeur(s) : Université des Antilles Études caribéennes
Résumé : El desbordamiento ocasionado por el modelo desarrollista de nuestra economía dio lugar a que el gobierno canario promoviera el Decreto (4/2001) para formular las Directrices de Ordenación General y del Turismo por entender que el territorio y los recursos naturales, en general, y la actividad turística, en particular, precisaban de una acción que articulase la orientación del desarrollo insular hacia un modelo sostenible. Se acompañó esa intervención con una serie de medidas cautelares tendentes a congelar el crecimiento y suspender el otorgamiento de licencias para la creación de nuevos establecimientos turísticos alojativos. Poco después, el ejecutivo canario emitió el Decreto 126/2001 para contener el crecimiento, y, finalmente, el Parlamento aprueba y pone en vigor la Ley de Medidas Urgentes en Materia de Ordenación del Territorio y del Turismo de Canarias (julio, 2001) para regular las condiciones que deben reunir los establecimientos de la modalidad hotelera con la máxima categoría. Por último, los diputados regionales dan el visto bueno a la Ley 19/2003 de Directrices de Ordenación General y las Directrices de Ordenación del Turismo de Canarias.
Confronté à une surfréquentation touristique, le Gouvernement local des Îles Canaries a publié un Arrêt (4/2001) qui reformule les Orientations Générales du Territoire et du Tourisme. L’objectif est de s’entendre sur les bases d’un modèle de développement insulaire soutenable, en mettant l’accent sur la gestion des ressources naturelles en général et le tourisme plus particulièrement. Cet Arrêt a été accompagné de normes dont l’objectif est de limiter la construction de nouveaux bâtiments hôteliers. Peu après, le Gouvernement a publié l’Arrêt 126/2001 qui limitait le développement touristique. Le Parlement adopta un autre Arrêt, la «Ley de Medidas Urgentes en Materia de Ordenación del Territorio y del Turismo de Canarias » (juillet 2001) qui détermine les conditions exigées aux établissements hôteliers de plus haute catégorie. Le Parlement donne aussi son accord à l’Arrêt 19/2003, «Directrices de Ordenación General y Directrices de Ordenación del Turismo de Canarias ».
Faced with excesses tourism, the local government of the Canary Islands issued a judgement (4 / 2001), which reformulates General Guidelines of Territory and Tourism. The aim is to set up the basis of a model of sustainable island development, with emphasis on natural resource management in general and tourism in particular. This decision was accompanied by standards whose objective is to limit the construction of new buildings for hotels. Shortly thereafter, the Government issued the ruling 126/2001 which limited the development of tourism. Parliament adopted another decree, "Ley de Medidas Urgent en Materia de Ordenación del Territorio y del Turismo de Canarias" (July 2001) which determines the conditions required for hotels higher category. The Parliament also gives its agreement to stop 19/2003, "Directrices de Ordenación General y Directrices de Ordenación del Turismo de Canarias."
Canaries

Droits : info:eu-repo/semantics/openAccess
urn:doi:10.4000/etudescaribeennes.1232
http://journals.openedition.org/etudescaribeennes/1232

Éditeur(s) : HAL CCSD Elsevier
Résumé : International audience
In this work we perform a statistical analysis of global solar radiation measured at two sites, 38 km distant from, in Guadeloupe, French West Indies. We have established a correlation model based on the coefficient of variation assuming a time scale separation. The coefficient of variation is calculated on minutes interval with data measured at 1 Hz. This analysis highlight the dynamic correlation that can occur between measurements from two different sites with a time step of one second. From this results, knowing the coefficient of variation on a site we have established a new correlation model on this parameter for another site. A diagram linking the standard deviation on both sites, for a given coefficient of variation is proposed for correlated and non-correlated cases. More over this analysis put in evidence the existence of a threshold time under which there is no significant correlation. This model can be useful in choosing new sites of production not only for establishing correlation from on site to another, but also in optimizing the geographical distribution of the solar farms within the territory.
ISSN: 0960-1481

Droits : http://hal.archives-ouvertes.fr/licences/copyright/
hal-01099045
https://hal.archives-ouvertes.fr/hal-01099045
https://hal.archives-ouvertes.fr/hal-01099045/document
https://hal.archives-ouvertes.fr/hal-01099045/file/Article%20Foison_2014.pdf

Résumé : Une étude de faisabilité de poutres en bois lamellé-collé a été menée en Guyane Française en utilisant les essences locales. Le but est de déterminer les paramètres de collage assurant la bonne résistance mécanique des assemblages par lamellation et par aboutage, nécessaires à la fabrication de poutres en bois lamellé-collé en climat tropical. Trois essences de bois abondantes de différentes densités ont été sélectionnées. Les adhésifs de types Mélamine-Urée-Formol et Résorcinol-Phénol-Formol ont été utilisées. Les paramètres de collage considérés sont : le grammage, le temps d’assemblage fermé et la pression.En utilisant les normes Européennes, plusieurs campagnes de tests ont été conduites sur des échantillons normalisés. Concernant le collage par lamellation, des tests élémentaires de délamination et de cisaillement des joints de colle ont été réalisés. Le test de délamination consiste en deux cycles d’immersion dans l’eau sous pression et de séchage, il induit de sévères variations d’humidité du bois susceptibles de créer des ouvertures des joints de colles. Le collage par aboutage est réalisé sur des poutres normalisées aboutées. Les entures sont obtenues grâce à un outillage standard communément utilisé pour les résineux. Les résultats ont mis en évidence l’influence des propriétés du bois et des paramètres de collage sur la résistance du joint et la résistance mécanique du produit. En effet, les paramètres du bois, tels que la densité, la rétractibilité et l’imprégnation, ont une forte influence sur le collage, en particulier sur la pression et le grammage nécessaires à la production d’un produit commercialisable. Il apparait qu’un bois de densité moyenne et poreux nécessite un grammage et une pression élevés comparé à un bois de densité élevée qui nécessite des valeurs moindres. Aussi, l’influence d’autres paramètres de fabrication, tels que le rabotage, l’encollage double face et simple face ainsi que l’épaisseur des lamelles, a été établie. Par contre il semblerait que le matériel communément utilisé pour l’aboutage des résineux n’est pas adapté aux feuillus tropicaux. En final, des paramètres de collage ont été validés pour la fabrication de lamellé-collé endémique en Guyane Française et une étude du coût de production d’une poutre lamellé-collé standard a identifié les conditions économiques d’une fabrication en Guyane Française.
A feasibility study of glued-laminated timber beams was carried out in French Guiana using local wood species. The aim of the study was to evaluate the gluing parameters that could ensure good mechanical properties for the manufactured glued-laminated beams in tropical climates. Three abundant wood species with different specific gravity were selected for the study. Melamine-Urea-Formaldehyde and Resorcinol-Phenol-Formaldehyde resins were used for gluing. The three gluing parameters considered are: adhesive spread rate, closing assembly time and gluing pressure.Using European standards, several laboratory tests were performed on normalized samples. The evaluation of a successful lamella’s gluing was done by using elementary tests of delamination and shearing. A delamination test consists of two cycles of water immersion at high pressure and drying which induces severe hygroscopic variation in wood thus causing joints opening. The finger-joints are made using a standard tool usually used for cutting softwoods. The performed tests revealed the influence of wood properties and the gluing parameters on the joint resistance and the mechanical properties of the product. Results show that wood properties such as specific gravity, shrinkage coefficient, and permeation, have a high degree of influence on the gluing step especially on the gluing pressure and on the adhesive spread rate needed to produce a commercial product. Furthermore, wood with medium specific gravity needs more adhesive and more pressure than the one with high specific gravity. Additionally, the effects of other manufacturing parameters, such as planning, double or simple side glue spreading and lamellae thicknesses, on the glue joint resistance were demonstrated. Finally, the common tools used for softwoods are not adapted to making the finger joints with tropical hardwoods.In conclusion, optimal gluing parameters for manufacturing glued-laminated timber beams in French Guiana were established and an economical study using standard glued-laminated timber beams revealed the economic conditions under which a successful manufacturing of these local beams can be implemented in French Guiana.

http://www.theses.fr/2011AGUY0484/document

Éditeur(s) : HAL CCSD Wiley
Résumé : [b]1.[/b] Plant functional traits, in particular specific leaf area (SLA), wood density and seed mass, are often good predictors of individual tree growth rates within communities. Individuals and species with high SLA, low wood density and small seeds tend to have faster growth rates.[br/][br/][b]2.[/b] If community-level relationships between traits and growth have general predictive value, then similar relationships should also be observed in analyses that integrate across taxa, biogeographic regions and environments. Such global consistency would imply that traits could serve as valuable proxies for the complex suite of factors that determine growth rate, and, therefore, could underpin a new generation of robust dynamic vegetation models. Alternatively, growth rates may depend more strongly on the local environment or growth–trait relationships may vary along environmental gradients.[br/][br/][b]3.[/b] We tested these alternative hypotheses using data on 27 352 juvenile trees, representing 278 species from 27 sites on all forested continents, and extensive functional trait data, 38% of which were obtained at the same sites at which growth was assessed. Data on potential evapotranspiration (PET), which summarizes the joint ecological effects of temperature and precipitation, were obtained from a global data base.[br/][br/][b]4.[/b] We estimated size-standardized relative height growth rates (SGR) for all species, then related them to functional traits and PET using mixed-effect models for the fastest growing species and for all species together.[br/][br/][b]5.[/b] Both the mean and 95th percentile SGR were more strongly associated with functional traits than with PET. PET was unrelated to SGR at the global scale. SGR increased with increasing SLA and decreased with increasing wood density and seed mass, but these traits explained only 3.1% of the variation in SGR. SGR–trait relationships were consistently weak across families and biogeographic zones, and over a range of tree statures. Thus, the most widely studied functional traits in plant ecology were poor predictors of tree growth over large scales.[br/][br/][b]6.[/b] Synthesis. We conclude that these functional traits alone may be unsuitable for predicting growth of trees over broad scales. Determining the functional traits that predict vital rates under specific environmental conditions may generate more insight than a monolithic global relationship can offer.
ISSN: 0022-0477

hal-01204226
https://hal.archives-ouvertes.fr/hal-01204226
DOI : 10.1111/1365-2745.12401
PRODINRA : 307144

Éditeur(s) : Université des Antilles Études caribéennes
Résumé : The tropical coast of Queensland, Australia is experiencing rapid population and tourism growth. Heavy development pressure is being placed on the biophysical environment, which includes the largest sand island in the world (World Heritage listed), Fraser Island. Despite the existence of State planning legislation oriented towards environmental sustainability, it is apparent that little, if any, regard is being paid to the long-term effects of resort and canal-estate development on the underlying biophysical environment of the coast and islands. While the remarkable surface features of the tropical coastal environment such as the surf beaches, the unique dune lakes, and the coastal vegetation, as well as the prolific wildlife, are used as selling points for new settlement and above all tourism, little is known or apparently of major concern with respect to the long term environmental impact of coastal development. While the crowding effect of large numbers of visitors and their impact on the natural environment - through significant degradation, site hardening or lack of proper on-site management – has been identified as a particular problem in certain areas, there is little knowledge of impacts on the underlying coastal geomorphology. In particular, developmental choices are often made in ignorance of, or disregard of, the widespread existence of tropical acid sulfate soils in that coastal environment. Reactive soils such as these can, and do, greatly affect the biophysical outcomes of a development if they are exposed. This paper documents such impacts in the form of a case study of the acid sulfate soil problem in relation to the development of tourist resorts, and suggests that part of the solution is for the State Government to insist on enforceable standards relating to their development for tourism in the coastal zone. These would reduce the use by many resort owners of fancy golf course developments as an environmental cover up to make it look as if they put a lot of effort into protecting the environment….
La côte tropicale du Queensland est confrontée à un accroissement rapide de la population. Les fortes pressions anthropiques pèsent sur le milieu biophysique, qui comprend notamment a plus grande île de sable du monde, un site classé Patrimoine de l’Humanité, Port Hinchinbrook et Fraser Island. Malgré l’existence d’une législation nationale orientée vers la préservation de l’environnement, il est manifeste que peu d’attention est accordée aux effets à long terme des sites touristiques et du développement de l’urbanisation sur ces franges côtières sensibles. L’aspect massif des flux de visiteurs et leurs impacts sur le milieu naturel a été identifié comme un sujet d’inquiétude particulier dans certaines régions. La connaissance des impacts sur la géomorphologie côtière souterraine est très réduite et les choix de développement sont souvent faits dans l’ignorance ou au mépris de la présence répandue de terrains de sulfate d’acide dans ce milieu côtier. Des sols réactifs comme ceux-là peuvent affecter, et affectent, grandement les résultats géophysiques d’un développement lorsqu’ils sont connus. Des études de cas sur le problème des sols de sulfate d’acide suggèrent qu’une partie de la solution pour le gouvernement de l’Etat du Queensland est d’imposer des normes obligatoires pour le développement du tourisme dans la zone côtière.
Australie
Queensland

Droits : info:eu-repo/semantics/openAccess
urn:doi:10.4000/etudescaribeennes.1272
http://journals.openedition.org/etudescaribeennes/1272

Éditeur(s) : HAL CCSD Elsevier
Résumé : International audience
Predicting the impact of underground engineering on the environment requires the knowledge of natural media at different scales. In particular, understanding basic phenomena controlling the properties of rocks in the presence of complex fluids necessitates a detailed atomic description of the solid/fluid/solid contacts, the subject of Part I of the present study. First, building the solid interspace between two different crystals in a non-periodic situation is achieved using the ab initio and molecular mechanics code GenMol (TM). A description of the fluid confined within the interspace is then derived from the original genetic iterative multi-species (GIMS) algorithm implemented in the same code. This approach consists of equilibrating chemical potentials, cycle after cycle and species after species, between the confined fluid and the free natural fluid. An elementary iteration for a species k consists of different steps incrementing the number N-k of particles k, with other numbers N-k remaining constant. At each step, an optimum fluid composition is obtained by a genetic process distributing the fluid particles on a grid by stochastic shots, followed in fine by a refining process. The effectiveness of the GIMS approach is demonstrated in the case study of a fluid confined between two (0 0 1) kaolinite faces, with apertures h varying between 4 and 10 A. connected to a 9-species external solution [H2O, Cl-, Na+ CO2(aq), NaCl(aq), Ca2+, Mg2+, HCO3-, H3O+] where concentrations are ranging from 55 to 10(-4) mol/L. The results show a drastic variation in the solute/solvent and cations/ions ratios in the confined fluid when aperture h is lowered to less than 1 nm. These results obtained with a very rapid convergence of the iterative algorithm combined with a very competitive genetic optimizer are validated with high precision on a free solution. This description of contacts between crystals is original and unattainable by standard crystal interface approaches. It opens the way to understanding and/or predicting phenomena between crystals in a complex natural environment, such as adhesion or repulsion investigated in the Part II of this study.
ISSN: 0022-0248

hal-00544569
https://hal.archives-ouvertes.fr/hal-00544569
DOI : 10.1016/j.jcrysgro.2010.08.009

Éditeur(s) : HAL CCSD Wiley
Résumé : The allocation and cycling of carbon (C) within forests is an important component of the biospheric C cycle, but is particularly understudied within tropical forests. We synthesise reported and unpublished results from three lowland rainforest sites in Amazonia (in the regions of Manaus, Tapajos and Caxiuana), all major sites of the Large-Scale Biosphere-Atmosphere Programme (LBA). We attempt a comprehensive synthesis of the C stocks, nutrient status and, particularly, the allocation and internal C dynamics of all three sites. The calculated net primary productivities (NPP) are 10.1 +/- 1.4 Mg C ha(-1) yr(-1) (Manaus), 14.4 +/- 1.3 Mg C ha(-1) yr(-1) (Tapajos) and 10.0 +/- 1.2 Mg C ha(-1) yr(-1) (Caxiuana). All errors bars report standard errors. Soil and leaf nutrient analyses indicate that Tapajos has significantly more plant-available phosphorus and calcium. Autotrophic respiration at all three sites (14.9-21.4 Mg C ha yr(-1)) is more challenging to measure, with the largest component and greatest source of uncertainty being leaf dark respiration. Comparison of measured soil respiration with that predicted from C cycling measurements provides an independent constraint. It shows general good agreement at all three sites, with perhaps some evidence for measured soil respiration being less than expected. Twenty to thirty percent of fixed C is allocated belowground. Comparison of gross primary productivity (GPP), derived from ecosystem flux measurements with that derived from component studies (NPP plus autotrophic respiration) provides an additional crosscheck. The two approaches are in good agreement, giving increased confidence in both approaches to estimating GPP. The ecosystem carbon-use efficiency (CUEs), the ratio of NPP to GPP, is similar at Manaus (0.34 +/- 0.10) and Caxiuana (0.32 +/- 0.07), but may be higher at Tapajos (0.49 +/- 0.16), although the difference is not significant. Old growth or infertile tropical forests may have low CUE compared with recently disturbed and/or fertile forests.
ISSN: 1354-1013

hal-01032150
https://hal.archives-ouvertes.fr/hal-01032150
DOI : 10.1111/j.1365-2486.2008.01780.x

Éditeur(s) : Gauthier-Villars
Résumé : The Scopoletin-Peroxidase fluorescence decay method has been developed using standard additions and a flow injection manifold for hydrogen peroxide (H2O2) determination in marine waters. The limit of detection of the method was optimized using stored milli-Q water with a minimum of 5 nM (3 sigma). The method was found to be linear from 5 to 700 nM, with an approximate relative standard deviation of 10%. This accuracy was verified on both milli-Q and natural waters running systematically duplicate analysis. The validity and the sensitivity of the technique was studied through the influence of pH, salinity and the dissolved organic matter (DOM) concentration. Standard additions avoided all matrix and experimental interferences, making it possible to use this method under any field conditions and on ''humic-rich'' waters. Additionally, the flow injection manifold improved the accuracy and the runtime of the analytical procedure and simplified the field manipulation.
Oceanologica Acta (0399-1784) (Gauthier-Villars), 1995 , Vol. 18 , N. 3 , P. 353-361

Droits : info:eu-repo/semantics/openAccess
http://archimer.ifremer.fr/doc/00097/20784/18409.pdf
http://archimer.ifremer.fr/doc/00097/20784/