Éditeur(s) : HAL CCSD Landes Bioscience
Résumé : International audience
In 2008 we published the first set of guidelines for standardizing research in autophagy. Since then, research on this topic has continued to accelerate, and many new scientists have entered the field. Our knowledge base and relevant new technologies have also been expanding. Accordingly, it is important to update these guidelines for monitoring autophagy in different organisms. Various reviews have described the range of assays that have been used for this purpose. Nevertheless, there continues to be confusion regarding acceptable methods to measure autophagy, especially in multicellular eukaryotes. A key point that needs to be emphasized is that there is a difference between measurements that monitor the numbers or volume of autophagic elements (e.g., autophagosomes or autolysosomes) at any stage of the autophagic process vs. those that measure flux through the autophagy pathway (i.e., the complete process); thus, a block in macroautophagy that results in autophagosome accumulation needs to be differentiated from stimuli that result in increased autophagic activity, defined as increased autophagy induction coupled with increased delivery to, and degradation within, lysosomes (in most higher eukaryotes and some protists such as Dictyostelium) or the vacuole (in plants and fungi). In other words, it is especially important that investigators new to the field understand that the appearance of more autophagosomes does not necessarily equate with more autophagy. In fact, in many cases, autophagosomes accumulate because of a block in trafficking to lysosomes without a concomitant change in autophagosome biogenesis, whereas an increase in autolysosomes may reflect a reduction in degradative activity. Here, we present a set of guidelines for the selection and interpretation of methods for use by investigators who aim to examine macroautophagy and related processes, as well as for reviewers who need to provide realistic and reasonable critiques of papers that are focused on these processes. These guidelines are not meant to be a formulaic set of rules, because the appropriate assays depend in part on the question being asked and the system being used. In addition, we emphasize that no individual assay is guaranteed to be the most appropriate one in every situation, and we strongly recommend the use of multiple assays to monitor autophagy. In these guidelines, we consider these various methods of assessing autophagy and what information can, or cannot, be obtained from them. Finally, by discussing the merits and limits of particular autophagy assays, we hope to encourage technical innovation in the field.
Autophagy

hal-00735751
https://hal.archives-ouvertes.fr/hal-00735751
PUBMED : 22966490

Résumé : This report includes a qualitative and descriptive approach of the oyster and mussel industries in the European Community. This report compares the structure of production, the marketsn the professional organisation, competition for the resource, legislation and support systemat the community levell. Technical and statistical datas are summarized to représent the structure of the industry, the trade flows and the main evolutions of the two industries in the EC. A summary of the county reports gives an overview of the present state of the industry in each country and suggest a first evaluation of constraints and prospects. [NOT CONTROLLDED OCR]
Ce rapport comprend une approche descriptive et qualitative du secteur conchylicole traditionnel (moules et huîtres) dans la Communauté Européenne. Il aborde les aspects production, marché, organisation, réglementation, système d'aide dans les différents pays par une analyse comparative des secteurs, complétée par une synthèse chiffrée des données techniques et statistiques. Une vision d'ensemble des flux et de l'évolution des deux filières dans la Communauté est ainsi présentée. Un résumé des rapports nationaux évalue l'organisation, les contraintes et les perspectives du secteur dans chacun des pays. [OCR NON CONTRÔLE]

Droits : 1993 Ifremer
http://archimer.ifremer.fr/doc/1993/rapport-4176.pdf
http://archimer.ifremer.fr/doc/1993/sup-4176.pdf
http://archimer.ifremer.fr/doc/00000/4176/

Éditeur(s) : HAL CCSD Elsevier
Résumé : International audience
This study analyzes the active tectonics within the northwestern and southeastern extensions of the Pambak-Sevan-Syunik fault (PSSF), a major right-lateral strike-slip fault cutting through Armenia. Quantifying the deformations in terms of geometry, kinematics, slip rates and earthquake activity, using cosmogenic 3He, OSL/IRSL and radiocarbon dating techniques, reveal different behaviors between the two regions. Within the northwestern extension, in the region of Amasia, the PSSF bends to the west and splits into two main WNW–ESE trending reverse faults defining a compressional pop-up structure. We estimate an uplift rate and a shortening rate of 0.5 ± 0.1 mm/y and 1.4 ± 0.6 mm/y, respectively. This suggests that most of the ∼2 mm/y right lateral movement of the PSSF seems to be absorbed within the Amasia pop-structure. Within the southeastern extension, the PSSF shows signs of dying out within the Tsghuk Volcano region at the southernmost tip of the Syunik graben. There, the tectonic activity is characterized by a very slow NS trending normal faulting associated with a slight right-lateral movement. Slip rates analyses (i.e. vertical slip rate, EW stretching rate at 90° to the fault, and right-lateral slip rate of ∼0.2 mm/y, ∼0.1 mm/y and ∼0.05 mm/y, respectively) lead to the conclusion that the right lateral movement observed further north along the PSSF is mainly transferred within other active faults further west within the Karabagh (Hagari fault or other structures further northwestwards). Comparing our slip rates with those estimated from GPS data suggests that most of the deformation is localized and seismic, at least within the Tsghuk region. The geometrical and kinematic pattern observed within the two terminations of the PSSF suggests that the fault and its surrounding crustal blocks are presently rotating anticlockwise, as also observed within the GPS velocity field. This is consistent with the recent kinematic models proposed for the Caucasus-Kura-South Caspian region and brings a new insight into the present geodynamics of Armenia.
ISSN: 1040-6182

hal-01307342
https://hal.archives-ouvertes.fr/hal-01307342
DOI : 10.1016/j.quaint.2015.05.021

Éditeur(s) : IEEE
Résumé : It is now well understood that data on soil moisture and sea surface salinity (SSS) are required to improve meteorological and climate predictions. These two quantities are not yet available globally or with adequate temporal or spatial sampling. It is recognized that a spaceborne L-band radiometer with a suitable antenna is the most promising way of fulfilling this gap. With these scientific objectives and technical solution at the heart of a proposed mission concept the European Space Agency (ESA) selected the Soil Moisture and Ocean Salinity (SMOS) mission as its second Earth Explorer Opportunity Mission. The development of the SMOS mission was led by ESA in collaboration with the Centre National d'Etudes Spatiales (CNES) in France and the Centro para el Desarrollo Tecnologico Industrial (CDTI) in Spain. SMOS carries a single payload, an L-Band 2-D interferometric radiometer operating in the 1400-1427-MHz protected band [1]. The instrument receives the radiation emitted from Earth's surface, which can then be related to the moisture content in the first few centimeters of soil over land, and to salinity in the surface waters of the oceans. SMOS will achieve an unprecedented maximum spatial resolution of 50 km at L-band over land (43 km on average over the field of view), providing multiangular dual polarized (or fully polarized) brightness temperatures over the globe. SMOS has a revisit time of less than 3 days so as to retrieve soil moisture and ocean salinity data, meeting the mission's science objectives. The caveat in relation to its sampling requirements is that SMOS will have a somewhat reduced sensitivity when compared to conventional radiometers. The SMOS satellite was launched successfully on November 2, 2009.
Proceedings of the IEEE (0018-9219) (IEEE), 2010-05 , Vol. 98 , N. 5 , P. 666-687

Droits : 2010 IEEE – All Rights Reserved
http://archimer.ifremer.fr/doc/00004/11483/8065.pdf
DOI:10.1109/JPROC.2010.2043032
http://archimer.ifremer.fr/doc/00004/11483/

Éditeur(s) : HAL CCSD Wiley
Résumé : International audience
MR and MDHD contributed equally to this work. Summary Painful vaso-occlusive crisis, a hallmark of sickle cell anaemia, results from complex, incompletely understood mechanisms. Red blood cell (RBC) damage caused by continuous endogenous and exogenous oxidative stress may precipitate the occurrence of vaso-occlusive crises. In order to gain insight into the relevance of oxidative stress in vaso-occlusive crisis occurrence , we prospectively compared the expression levels of various oxidative markers in 32 adults with sickle cell anaemia during vaso-occlusive crisis and steady-state conditions. Compared to steady-state condition, plasma levels of free haem, advanced oxidation protein products and myeloperoxi-dase, RBC caspase-3 activity, as well as the concentrations of total, neutrophil-and RBC-derived microparticles were increased during vaso-occlusive crises, whereas the reduced glutathione content was decreased in RBCs. In addition, natural anti-band 3 autoantibodies levels decreased during crisis and were negatively correlated with the rise in plasma advanced oxidation protein products and RBC caspase-3 activity. These data showed an exacerbation of the oxidative stress during vaso-occlusive crises in sickle cell anaemia patients and strongly suggest that the higher concentration of harmful circulating RBC-derived microparticles and the reduced anti-band 3 autoantibodies levels may be both related to the recruitment of oxidized band 3 into membrane aggregates.
ISSN: 0007-1048

inserm-01476067
http://www.hal.inserm.fr/inserm-01476067
http://www.hal.inserm.fr/inserm-01476067/document
http://www.hal.inserm.fr/inserm-01476067/file/2016%2C%20Hierso%20-%20Exacerbation%20of%20oxidative%20stress%20during%20sickle%20vaso-occlusive%20crisis.pdf
DOI : 10.1111/bjh.14476

Éditeur(s) : HAL CCSD American Geophysical Union (AGU)
Résumé : International audience
[1] Zircons and monazites from 6 samples of the North Ayilari dextral shear zone (NAsz), part of the Karakorum fault zone (KFZ), have been dated with the U-Th-Pb method, using both ID-TIMS and SIMS techniques. The ages reveal (1) inheritance from several events spanning a long period between the late Archean and the Jurassic; (2) an Eocene-Oligocene magmatic event (similar to 35-32 Ma); (3) an Oligo-Miocene magmatic event (similar to 25-22 Ma), at least partly synkinematic to the right-lateral deformation; and (4) a period of metamorphism metasomatism (similar to 22-14 Ma) interpreted as thermal and fluid advection in the shear zone. The Labhar Kangri granite located similar to 375 km farther Southeast along the KFZ is dated at 21.1 +/- 0.3 Ma. Such occurrence of several Oligo-Miocene granites along the KFZ, some of which show evidence for synkinematic emplacement, suggests that the fault zone played an important role in the genesis and /or collection of crustal melts. We discuss several scenarios for the onset and propagation of the KFZ, and offset estimates based on the main sutures zones. Our preferred scenario is an Oligo-Miocene initiation of the fault close to the NA range, and propagation along most of its length prior to similar to 19 Ma. In its southern half, the averaged long-term fault-rate of the KFZ is greater than 8 to 10 mm/a, in good agreement with some shorter-term estimates based on the Indus river course, or Quaternary moraines and geodesy. Our results show the KFZ cannot be considered as a small transient fault but played a major role in the collision history.
ISSN: 0278-7407

hal-00412570
https://hal.archives-ouvertes.fr/hal-00412570
DOI : 10.1029/2007TC002184

Éditeur(s) : HAL CCSD
Résumé : International audience
AGU Fall Meeting 2014
San Francisco, United States

insu-01119759
https://hal-insu.archives-ouvertes.fr/insu-01119759

Éditeur(s) : HAL CCSD
Résumé : International audience
o abstract
Revue Des Maladies Respiratoires

hal-01647782
https://hal-univ-rennes1.archives-ouvertes.fr/hal-01647782
DOI : 10.1016/j.rmr.2017.07.017
PUBMED : 28939155

Éditeur(s) : HAL CCSD Nature Publishing Group
Résumé : International audience
Assessing seismic hazards remains one of the most challenging scientific issues in Earthsciences. Deep tectonic processes are classically considered as the only persistentmechanism driving the stress loading of active faults over a seismic cycle. Here we show via amechanical model that erosion also significantly influences the stress loading of thrust faultsat the timescale of a seismic cycle. Indeed, erosion rates of about B0.1–20mmyr1, asdocumented in Taiwan and in other active compressional orogens, can raise the Coulombstress by B0.1–10 bar on the nearby thrust faults over the inter-seismic phase. Masstransfers induced by surface processes in general, during continuous or short-lived andintense events, represent a prominent mechanism for inter-seismic stress loading offaults near the surface. Such stresses are probably sufficient to trigger shallow seismicity orpromote the rupture of deep continental earthquakes up to the surface.
ISSN: 2041-1723

insu-01087103
https://hal-insu.archives-ouvertes.fr/insu-01087103
https://hal-insu.archives-ouvertes.fr/insu-01087103/document
https://hal-insu.archives-ouvertes.fr/insu-01087103/file/Steer2014_NatureComm_accepted.pdf
DOI : 10.1038/ncomms6564

Éditeur(s) : HAL CCSD
2012 AGU Fall Meeting

hal-00771546
https://hal-mines-paristech.archives-ouvertes.fr/hal-00771546

Éditeur(s) : HAL CCSD
Résumé : International audience
o abstract
Revue Des Maladies Respiratoires

hal-01647076
https://hal-univ-rennes1.archives-ouvertes.fr/hal-01647076
DOI : 10.1016/j.rmr.2017.07.022
PUBMED : 28935495

Éditeur(s) : HAL CCSD Elsevier
Résumé : International audience
We present the 1998-2009 GPS-derived velocity field for the Armenia region based on a survey-mode observation network of 31 GPS sites.We combine our results with previous GPS studies of the region to better assess the deformation of the Lesser Caucasus and Kura basin region. The results show that the Kura basin and the Lesser Caucasus regions are two different blocks, and that the main fault (Pambak-Sevan-Sunik) between these two regions has a right-lateral slip rate of 2±1 mm/yr. This is consistentwithmorphotectonic estimates and suggests a fairly constant slip rate over the last 120-300 ka. The right-lateral slip rate on one of the southern branch of the Pambak-Sevan-Sunik fault is lower than 1 mm/yr and the good agreementwith a geologically estimated slip rate suggests a constant slip rate over the last 1.4 Myr. The Sardarapat and Akhurian faults experience some shortening. This shortening is consistentwith someindependent geological estimates and shows the Arabian push.However, NNW-SSE-orientated faults have an extensional fault normal component instead of the expected shortening due to the Arabia-Eurasia convergence. This substantial extensive strain, and the sharp azimuth change of the velocity vectors between the Arabia promontory and the Lesser Caucasus suggest that processes other than "extrusion", possibly related to old subduction or delamination, contribute to the geodynamics of the region.
ISSN: 0040-1951

hal-00812623
https://hal.archives-ouvertes.fr/hal-00812623
DOI : 10.1016/j.tecto.2013.02.002

Éditeur(s) : HAL CCSD Météo et Climat
Résumé : International audience
D'initiative française, le projet international HyMeX a pour objectif d'améliorer la compréhension du cycle de l'eau en Méditerranée, de sa variabilité, de l'échelle de l'événement météorologique aux échelles saisonnières et interannuelles, et de ses caractéristiques sur une décennie, dans un contexte de changement global. Le projet est motivé par le rôle déterminant des processus de mésoéchelle, couplés entre l'atmosphère, la mer et la terre, sur la variabilité du système climatique et sur le déclenchement d'événements hydrométéorologiques extrêmes (précipitations et inondations, vents forts et convection océanique, canicules et sécheresses). Le projet vise enfin à évaluer les conséquences de ces événements extrêmes sur la vulnérabilité sociale et économique de cette région et sa capacité d'adaptation.
ISSN: 0026-1181

hal-00828758
https://hal.archives-ouvertes.fr/hal-00828758
https://hal.archives-ouvertes.fr/hal-00828758/document
https://hal.archives-ouvertes.fr/hal-00828758/file/meteo_2013_80_23%20%281%29.pdf
DOI : 10.4267/2042/48792

Éditeur(s) : Oxford Univ Press
Résumé : This study presents the results of a deep seismic survey across the north Algerian margin, based on the combination of 2-D multichannel and wide-angle seismic data simultaneously recorded by 41 ocean bottom seismometers deployed along a north-south line extending 180 km off Jijel into the Algerian offshore basin, and 25 land stations deployed along a 100-km-long line, cutting through the Lesser Kabylia and the Tellian thrust-belt. The final model obtained using forward modelling of the wide-angle data and pre-stack depth migration of the seismic reflection data provides an unprecedented view of the sedimentary and crustal structure of the margin. The sedimentary layers in the Algerian basin are 3.75 km thick to the north and up to 4.5-5 km thick at the foot of the margin. They are characterized by seismic velocities from 1.9 to 3.8 km s(-1). Messinian salt formations are about 1 km thick in the study area, and are modelled and imaged using a velocity between 3.7 and 3.8 km s(-1). The crust in the deep sea basin is about 4.5 km thick and of oceanic origin, presenting two distinct layers with a high gradient upper crust (4.7-6.1 km s(-1)) and a low gradient lower crust (6.2-7.1 km s(-1)). The upper-mantle velocity is constrained to 7.9 km s(-1). The ocean-continent transition zone is very narrow between 15 and 20 km wide. The continental crust reaches 25 km thickness as imaged from the most landward station and thins to 5 km over a less than 70 km distance. The continental crust presents steep and asymmetric upper- and lower-crustal geometry, possibly due to either asymmetric rifting of the margin, an underplated body, or flow of lower crustal material towards the ocean basin. Present-time deformation, as imaged from three additional seismic profiles, is characterized by an interplay of gravity-driven mobile-salt creep and active thrusting at the foot of the tectonically inverted Algerian margin.
Geophysical Journal International (0956-540X) (Oxford Univ Press), 2014-09 , Vol. 198 , N. 3 , P. 1486-1503

Droits : 2014 The Royal Astronomical Society
http://archimer.ifremer.fr/doc/00217/32836/32150.pdf
DOI:10.1093/gji/ggu179
http://archimer.ifremer.fr/doc/00217/32836/

Éditeur(s) : HAL CCSD Wiley/Blackwell
Résumé : International audience
This article documents the addition of 512 microsatellite marker loci and nine pairs of Single Nucleotide Polymorphism (SNP) sequencing primers to the Molecular Ecology Resources Database. Loci were developed for the following species: Alcippe morrisonia morrisonia, Bashania fangiana, Bashania fargesii, Chaetodon vagabundus, Colletes floralis, Coluber constrictor flaviventris, Coptotermes gestroi, Crotophaga major, Cyprinella lutrensis, Danaus plexippus, Fagus grandifolia, Falco tinnunculus, Fletcherimyia fletcheri, Hydrilla verticillata, Laterallus jamaicensis coturniculus, Leavenworthia alabamica, Marmosops incanus, Miichthys miiuy, Nasua nasua, Noturus exilis, Odontesthes bonariensis, Quadrula fragosa, Pinctada maxima, Pseudaletia separata, Pseudoperonospora cubensis, Podocarpus elatus, Portunus trituberculatus, Rhagoletis cerasi, Rhinella schneideri, Sarracenia alata, Skeletonema marinoi, Sminthurus viridis, Syngnathus abaster, Uroteuthis (Photololigo) chinensis, Verticillium dahliae, Wasmannia auropunctata, and Zygochlamys patagonica. These loci were cross-tested on the following species: Chaetodon baronessa, Falco columbarius, Falco eleonorae, Falco naumanni, Falco peregrinus, Falco subbuteo, Didelphis aurita, Gracilinanus microtarsus, Marmosops paulensis, Monodelphis Americana, Odontesthes hatcheri, Podocarpus grayi, Podocarpus lawrencei, Podocarpus smithii, Portunus pelagicus, Syngnathus acus, Syngnathus typhle,Uroteuthis (Photololigo) edulis, Uroteuthis (Photololigo) duvauceli and Verticillium albo-atrum. This article also documents the addition of nine sequencing primer pairs and sixteen allele specific primers or probes for Oncorhynchus mykiss and Oncorhynchus tshawytscha; these primers and assays were cross-tested in both species.
ISSN: 1755-098X

hal-01094222
https://hal-agroparistech.archives-ouvertes.fr/hal-01094222
DOI : 10.1111/j.1755-0998.2010.02916.x

Éditeur(s) : HAL CCSD Endocrine Society
Résumé : International audience
The Developmental Origins of Health and Disease (DOHaD) paradigm is one of the most rapidly expanding areas of biomedical research. Environmental stressors that can impact on DOHaD encompass a variety of environmental and occupational hazards as well as deficiency and oversupply of nutrients and energy. They can disrupt early developmental processes and lead to increased susceptibility to disease/dysfunctions later in life. Presentations at the fourth Conference on Prenatal Programming and Toxicity in Boston, in October 2014, provided important insights and led to new recommendations for research and public health action. The conference highlighted vulnerable exposure windows that can occur as early as the preconception period and epigenetics as a major mechanism than can lead to disadvantageous "reprogramming" of the genome, thereby potentially resulting in transgenerational effects. Stem cells can also be targets of environmental stressors, thus paving another way for effects that may last a lifetime. Current testing paradigms do not allow proper characterization of risk factors and their interactions. Thus, relevant exposure levels and combinations for testing must be identified from human exposure situations and outcome assessments. Testing of potential underpinning mechanisms and biomarker development require laboratory animal models and in vitro approaches. Only few large-scale birth cohorts exist, and collaboration between birth cohorts on a global scale should be facilitated. DOHaD-based research has a crucial role in establishing factors leading to detrimental outcomes and developing early preventative/remediation strategies to combat these risks
ISSN: 0013-7227

hal-01187409
https://hal-univ-rennes1.archives-ouvertes.fr/hal-01187409
DOI : 10.1210/EN.2015-1350
PUBMED : 26241067

Éditeur(s) : HAL CCSD
Résumé : International audience
Geodynamics and Environment in East Asia Symposium (GEEA Meeting)
Hualien, Taiwan

insu-01119753
https://hal-insu.archives-ouvertes.fr/insu-01119753

Éditeur(s) : HAL CCSD Wiley-Blackwell
Résumé : International audience
UNLABELLED: Interleukin (IL)-33, a member of the IL-1 cytokine family, positively correlates with acute hepatitis and chronic liver failure in mice and humans. IL-33 is expressed in hepatocytes and is regulated by natural killer T (NKT) cells during concanavalin A (ConA)-induced acute liver injury. Here, we investigated the molecular mechanisms underlying the expression of IL-33 during acute hepatitis. The expression of IL-33 and its regulation by death receptor pathways was investigated after the induction of ConA-acute hepatitis in wildtype (WT), perforin(-/-) , tumor necrosis factor related apoptosis inducing ligand (TRAIL)(-/-) , and NKT cell-deficient (CD1d(-/-) ) mice. In addition, we used a model of acute liver injury by administering Jo2/Fas-antibody or D-galactosamine-tumor necrosis factor alpha (TNFα) in WT mice. Finally, the effect of TRAIL on IL-33 expression was assessed in primary cultured murine hepatocytes. We show that IL-33 expression in hepatocytes is partially controlled by perforin during acute liver injury, but not by TNFα or Fas ligand (FasL). Interestingly, the expression of IL-33 in hepatocytes is blocked during ConA-acute hepatitis in TRAIL-deficient mice compared to WT mice. In contrast, administration of recombinant murine TRAIL associated with ConA-priming in CD1d-deficient mice or in vitro stimulation of murine hepatocytes by TRAIL but not by TNFα or Jo2 induced IL-33 expression in hepatocytes. The IL-33-deficient mice exhibited more severe ConA liver injury than WT controls, suggesting a protective effect of IL-33 in ConA-hepatitis. CONCLUSION: The expression of IL-33 during acute hepatitis is dependent on TRAIL, but not on FasL or TNFα.
ISSN: 0270-9139

hal-00874194
https://hal.archives-ouvertes.fr/hal-00874194
PUBMED : 22961755
DOI : 10.1002/hep.25893

Éditeur(s) : HAL CCSD
Résumé : International audience
Geodynamics and Environment in East Asia Symposium (GEEA Meeting)
Hualien, Taiwan

insu-01119754
https://hal-insu.archives-ouvertes.fr/insu-01119754