Éditeur(s) : HAL CCSD
Résumé : International audience
Conférence MISTRALS PAELOMEX
Montpellier, France

hal-01683548
https://hal-univ-tlse2.archives-ouvertes.fr/hal-01683548

Éditeur(s) : HAL CCSD Public Library of Science
Résumé : International audience
Diagnosis of toxoplasmic encephalitis (TE) in patients with AIDS is not straightforward because clinicians rely initially on an empiric diagnosis based on clinical and radiographic improvement to specific anti-Toxoplasma gondii therapy. There is therefore a need for biological tools to improve the diagnosis of TE, especially in tropical areas where this diagnosis is likely to be underestimated. The use of PCR testing in blood samples for the diagnosis of TE has been limited by its poor sensitivity in the studies conducted in Europe. In tropical South America, the results of PCR sensitivity in blood samples were controversial. Considering that T. gondii strains from tropical South America have substantial genetic and pathogenic differences with those from USA and Europe, it is therefore important to re-evaluate the performance of the PCR assay in blood samples for the diagnosis of TE in AIDS patients from this region. Our results showed that the only interest of PCR would be in the most severe forms of TE with altered consciousness because PCR is more likely to be positive. We also provided important genotyping data on T. gondii strains isolated in human cases of toxoplasmosis in the Caribbean and in South America.
ISSN: 1935-2727

hal-01503761
https://hal-unilim.archives-ouvertes.fr/hal-01503761
https://hal-unilim.archives-ouvertes.fr/hal-01503761/document
https://hal-unilim.archives-ouvertes.fr/hal-01503761/file/journal.pntd.0004790.pdf
DOI : 10.1371/journal.pntd.0004790

Éditeur(s) : HAL CCSD Public Library of Science
Résumé : International audience
We assessed the occurrence of algae in five species of tank-bromeliads found in contrasting environmental sites in a Neotropical, primary rainforest around the Nouragues Research Station, French Guiana. The distributions of both algal abundance and biomass were examined based on physical parameters, the morphological characteristics of bromeliad species and with regard to the structure of other aquatic microbial communities held in the tanks. Algae were retrieved in all of the bromeliad species with mean densities ranging from ∼10(2) to 10(4) cells/mL. Their biomass was positively correlated to light exposure and bacterial biomass. Algae represented a tiny component of the detrital food web in shaded bromeliads but accounted for up to 30 percent of the living microbial carbon in the tanks of Catopsis berteroniana, located in a highly exposed area. Thus, while nutrient supplies are believed to originate from wind-borne particles and trapped insects (i.e., allochtonous organic matter), our results indicate that primary producers (i.e., autochtonous organic matter) are present in this insectivorous bromeliad. Using a 24-h incubation of size-fractionated and manipulated samples from this plant, we evaluated the impact of mosquito foraging on algae, other microorganisms and rotifers. The prey assemblages were greatly altered by the predation of mosquito larvae. Grazing losses indicated that the dominant algal taxon, Bumilleriopsis sp., like protozoa and rotifers, is a significant part of the diet of mosquito larvae. We conclude that algae are a relevant functional community of the aquatic food web in C. berteroniana and might form the basis of a complementary non-detrital food web.
ISSN: 1932-6203

hal-00819161
https://hal.archives-ouvertes.fr/hal-00819161
https://hal.archives-ouvertes.fr/hal-00819161/document
https://hal.archives-ouvertes.fr/hal-00819161/file/Brouard2011_AreAlgae.pdf
DOI : 10.1371/journal.pone.0020129

Éditeur(s) : HAL CCSD BioMed Central
Résumé : International audience
Background: HCV treatment uptake has drastically increased in HIV-HCV coinfected patients in France since direct-acting antiviral (DAA) treatment approval, resulting in HCV cure in 63% of all HIV-HCV patients by the end of 2015. We investigated the impact of scaling-up DAA on HCV prevalence in the whole HIV population and in various risk groups over the next 10 years in France using a transmission dynamic compartmental model.Methods: The model was based on epidemiological data from the French Dat’AIDS cohort. Eight risk groups wereconsidered, including high-risk (HR) and low-risk (LR) men who have sex with men (MSM) and male/female heterosexuals,intra-venous drug users, or patients from other risk groups. The model was calibrated on prevalence and incidence dataobserved in the cohort between 2012 and 2015.Results: On January 1, 2016, 156,811 patients were registered as infected with HIV in France (24,900 undiagnosedpatients) of whom 7938 (5.1%) had detectable HCV-RNA (722 undiagnosed patients). Assuming a treatmentcoverage (TC) rate of 30%/year (i.e., the observed rate in 2015), model projections showed that HCV prevalenceamong HIV patients is expected to drop to 0.81% in 2026. Sub-analyses showed a similar decrease of HIV-HCVprevalence in most risk groups, including LR MSM. Due to higher infection and reinfection rates, predicted prevalencein HR MSM remained stable from 6.96% in 2016 to 6.34% in 2026. Increasing annual TC rate in HR MSM to 50/70%would decrease HCV prevalence in this group to 2.35/1.25% in 2026. With a 30% TC rate, undiagnosed patients wouldaccount for 34% of HCV infections in 2026.Conclusions: Our model suggests that DAA could nearly eliminate coinfection in France within 10 years for most riskgroups, including LR MSM. Elimination in HR MSM will require increased TC.
ISSN: 1741-7015

Droits : info:eu-repo/semantics/OpenAccess
inserm-01685739
http://www.hal.inserm.fr/inserm-01685739
http://www.hal.inserm.fr/inserm-01685739/document
http://www.hal.inserm.fr/inserm-01685739/file/2017%2C%20Virlogeux%20-%20Modeling%20HIV-HCV%20coinfection.pdf
DOI : 10.1186/s12916-017-0979-1

Éditeur(s) : HAL CCSD Wiley
Résumé : International audience
Aim To examine variation in the phylogenetic diversity (PD) of tree communities across geographical and environmental gradients in Amazonia.Location Two hundred and eighty-three c.1ha forest inventory plots from across Amazonia.Methods We evaluated PD as the total phylogenetic branch length across species in each plot (PDss), the mean pairwise phylogenetic distance between species (MPD), the mean nearest taxon distance (MNTD) and their equivalents standardized for species richness (ses.PDss, ses.MPD, ses.MNTD). We compared PD of tree communities growing (1) on substrates of varying geological age; and (2) in environments with varying ecophysiological barriers to growth and survival.Results PDss is strongly positively correlated with species richness (SR), whereas MNTD has a negative correlation. Communities on geologically young- and intermediate-aged substrates (western and central Amazonia respectively) have the highest SR, and therefore the highest PDss and the lowest MNTD. We find that the youngest and oldest substrates (the latter on the Brazilian and Guiana Shields) have the highest ses.PDss and ses.MNTD. MPD and ses.MPD are strongly correlated with how evenly taxa are distributed among the three principal angiosperm clades and are both highest in western Amazonia. Meanwhile, seasonally dry tropical forest (SDTF) and forests on white sands have low PD, as evaluated by any metric.Main conclusions High ses.PDss and ses.MNTD reflect greater lineage diversity in communities. We suggest that high ses.PDss and ses.MNTD in western Amazonia results from its favourable, easy-to-colonize environment, whereas high values in the Brazilian and Guianan Shields may be due to accumulation of lineages over a longer period of time. White-sand forests and SDTF are dominated by close relatives from fewer lineages, perhaps reflecting ecophysiological barriers that are difficult to surmount evolutionarily. Because MPD and ses.MPD do not reflect lineage diversity per se, we suggest that PDss, ses.PDss and ses.MNTD may be the most useful diversity metrics for setting large-scale conservation priorities.
ISSN: 1366-9516

hal-01557462
https://hal.univ-lorraine.fr/hal-01557462
DOI : 10.1111/ddi.12357

Éditeur(s) : HAL CCSD Nature Publishing Group
Résumé : While Amazonian forests are extraordinarily diverse, the abundance of trees is skewed strongly towards relatively few 'hyperdominant' species. In addition to their diversity, Amazonian trees are a key component of the global carbon cycle, assimilating and storing more carbon than any other ecosystem on Earth. Here we ask, using a unique data set of 530 forest plots, if the functions of storing and producing woody carbon are concentrated in a small number of tree species, whether the most abundant species also dominate carbon cycling, and whether dominant species are characterized by specific functional traits. We find that dominance of forest function is even more concentrated in a few species than is dominance of tree abundance, with only ≈1% of Amazon tree species responsible for 50% of carbon storage and productivity. Although those species that contribute most to biomass and productivity are often abundant, species maximum size is also influential, while the identity and ranking of dominant species varies by function and by region.
ISSN: 2041-1723

hal-01204225
https://hal.archives-ouvertes.fr/hal-01204225
DOI : 10.1038/ncomms7857
PRODINRA : 305614
PUBMED : 25919449

Éditeur(s) : HAL CCSD Springer Verlag (Germany)
Résumé : International audience
Surface displacements due to temporal changes in environmental mass redistributions are observable in the coordinate time series of many Global Navigation Satellite System (GNSS) sites. In this study, we investigated the effect of loading on estimates of tectonic velocity computed from campaign-style GNSS observations. The study region is in the Pyrenees mountain range between France and Spain (ResPyr campaigns). In this area, seismic activity is continuous and moderate and the expected amplitude of the horizontal tectonic velocity is less than 0.5 mm/yr. In order to determine the velocity, 4 sparse GNSS campaigns were carried out from 1995 to 2010. Considering this small rate of deformation, loading phenomena can contribute a non-negligible artifact to the velocity computation that could affect our geodynamical interpretation. In this investigation, we specifically considered the atmospheric, hydrological, and non-tidal ocean loading phenomena. The computed loading deformations for this region show the horizontal displacements are dominated by the non-tidal ocean loading (maximum 4 mm for the North and 3.1 mm for the East components); the main vertical contributions are due to the atmospheric and continental water storage loading (maximum 14.3 for the atmosphere and 8.1 mm for the hydrology, respectively). We have found that the dominant loading effect on the horizontal velocity is the non-tidal ocean loading (mean of 0.11 mm/yr), whereas the vertical component is dominated by the hydrological loading (mean of 0.21 mm/yr). Since the study area is in a mountainous region, we also analyzed the difference between the atmospheric and the topography dependent atmospheric loading models at our GNSS campaign sites. We did not find any significant difference between the two atmospheric loading models in terms of horizontal velocity. Finally, we performed simulations to identify the optimum timing and frequency of future GNSS campaigns in this area that would minimize the loading effects on tectonic velocity estimates.
ISSN: 0039-3169

hal-00963629
https://hal.archives-ouvertes.fr/hal-00963629
DOI : 10.1007/s11200-012-0458-2

Éditeur(s) : HAL CCSD SAGE Publications
Résumé : International audience
The distribution of redox-sensitive elements (V, U, Mo, Mn) was studied in a sedimentary archive of the Pierre-Blanche lagoon encompassing 3500 years of sedimentation. Between 240 and 255 cm (i.e. between 0 and 350 cal. ad) in the core, there was a dark layer with no mollusc shells. Enrichments of V, U and Mo and depletion of Mn were observed in this layer compared with overlying and underlying sediments. This was attributed to the occurrence of anoxia in bottom water or at the sediment/water interface during the Roman period. This episode was concomitant with the formation of a sandy barrier, which now isolates the lagoon from the sea. Our results suggest that the isolation of the lagoon, which was deeper in the period concerned than at present, favoured stagnation of the water. Combined with the excess nutrient inputs because of human activities in Lattara harbour, stagnation may have caused anoxia in bottom water at least in some parts of the Palavasian lagoon system. Bottom conditions nevertheless remained sufficiently reducing for the accumulation of U and Mo to persist until around 800 cal. ad. Although the lagoon has been hyper-eutrophic since at least the middle of the 20th century and although anoxia occurs frequently in summer, redox proxies show no evidence for their occurrence. The shallow water and associated resuspension and reoxidation events explain the lack of V, Mo and U enrichment in recent sediments.
ISSN: 0959-6836

hal-01062151
https://hal.archives-ouvertes.fr/hal-01062151

Éditeur(s) : HAL CCSD American Association for the Advancement of Science
Résumé :  
Amazon forests are a key but poorly understood component of the global carbon cycle. If, as anticipated, they dry this century, they might accelerate climate change through carbon losses and changed surface energy balances. We used records from multiple long-term monitoring plots across Amazonia to assess forest responses to the intense 2005 drought, a possible analog of future events. Affected forest lost biomass, reversing a large long-term carbon sink, with the greatest impacts observed where the dry season was unusually intense. Relative to pre-2005 conditions, forest subjected to a 100-millimeter increase in water deficit lost 5.3 megagrams of aboveground biomass of carbon per hectare. The drought had a total biomass carbon impact of 1.2 to 1.6 petagrams (1.2 × 1015 to 1.6 × 1015 grams). Amazon forests therefore appear vulnerable to increasing moisture stress, with the potential for large carbon losses to exert feedback on climate change.
ISSN: 0036-8075

hal-01032111
https://hal.archives-ouvertes.fr/hal-01032111
DOI : 10.1126/science.1164033

Éditeur(s) : HAL CCSD Météo et Climat
Résumé : International audience
D'initiative française, le projet international HyMeX a pour objectif d'améliorer la compréhension du cycle de l'eau en Méditerranée, de sa variabilité, de l'échelle de l'événement météorologique aux échelles saisonnières et interannuelles, et de ses caractéristiques sur une décennie, dans un contexte de changement global. Le projet est motivé par le rôle déterminant des processus de mésoéchelle, couplés entre l'atmosphère, la mer et la terre, sur la variabilité du système climatique et sur le déclenchement d'événements hydrométéorologiques extrêmes (précipitations et inondations, vents forts et convection océanique, canicules et sécheresses). Le projet vise enfin à évaluer les conséquences de ces événements extrêmes sur la vulnérabilité sociale et économique de cette région et sa capacité d'adaptation.
ISSN: 0026-1181

hal-00828758
https://hal.archives-ouvertes.fr/hal-00828758
https://hal.archives-ouvertes.fr/hal-00828758/document
https://hal.archives-ouvertes.fr/hal-00828758/file/meteo_2013_80_23%20%281%29.pdf
DOI : 10.4267/2042/48792

Éditeur(s) : HAL CCSD
Résumé : International audience
EGU General Assembly 2015
Vienne, Austria

hal-01286324
https://hal.archives-ouvertes.fr/hal-01286324
BIBCODE : 2015EGUGA..1713337U

Éditeur(s) : HAL CCSD Elsevier
Résumé : International audience
According to the World Health Organization, around 20% of all cancers would be due to environmental factors. Among these factors, several chemicals are indeed well recognized carcinogens. The widespread contaminant benzo[a]pyrene (B[a]P), an often used model carcinogen of the polycyclic aromatic hydrocarbons' family, has been suggested to target most, if not all, cancer hallmarks described by Hanahan and Weinberg. It is classified as a group I carcinogen by the International Agency for Research on Cancer; however, the precise intracellular mechanisms underlying its carcinogenic properties remain yet to be thoroughly defined. Recently, the pH homeostasis, a well known regulator of carcinogenic processes, was suggested to be a key actor in both cell death and Warburg-like metabolic reprogramming induced upon B[a]P exposure. The present review will highlight those data with the aim of favoring research on the role of H + dynamics in environmental carcinogenesis.
ISSN: 1044-579X

hal-01531077
https://hal-univ-rennes1.archives-ouvertes.fr/hal-01531077
https://hal-univ-rennes1.archives-ouvertes.fr/hal-01531077/document
https://hal-univ-rennes1.archives-ouvertes.fr/hal-01531077/file/Hardonniere%20et%20al.%20-%20Environmental%20carcinogenesis%20and%20pH%20homeostasis%20n.pdf
DOI : 10.1016/j.semcancer.2017.01.001

Éditeur(s) : HAL CCSD Elsevier
Résumé : International audience
We review the geodynamic evolution of the Aegea-Anatolia region and discuss strain localisation there over geological times. From Late Eocene to Present, crustal deformation in the Aegean backarc has localised progressively during slab retreat. Extension started with the formation of the Rhodope Metamorphic Core Complex (Eocene) and migrated to the Cyclades and the northern Menderes massif (Oligocene and Miocene), accommodated by crustal-scale detachments and a first series of core complexes (MCCs). Extension then localised in Western Turkey, the Corinth Rift and the external Hellenic arc after Messinian times, while the North Anatolian Fault penetrated the Aegean Sea. Through time the direction and style of extension have not changed significantly except in terms of localisation. The contributions of progressive slab retreat and tearing, basal drag, extrusion tectonics and tectonic inheritance are discussed and we favour a model (1) where slab retreat is the main driving engine, (2) successive slab tearing episodes are the main causes of this stepwise strain localisation and (3) the inherited heterogeneity of the crust is a major factor for localising detachments. The continental crust has an inherited strong heterogeneity and crustal-scale contacts such as major thrust planes act as weak zones or as zones of contrast of resistance and viscosity that can localise later deformation. The dynamics of slabs at depth and the asthenospheric flow due to slab retreat also have influence strain localisation in the upper plate. Successive slab ruptures from the Middle Miocene to the Late Miocene have isolated a narrow stripe of lithosphere, still attached to the African lithosphere below Crete. The formation of the North Anatolian Fault is partly a consequence of this evolution. The extrusion of Anatolia and the Aegean extension are partly driven from below (asthenospheric flow) and from above (extrusion of a lid of rigid crust).
ISSN: 0040-1951

insu-00715950
https://hal-insu.archives-ouvertes.fr/insu-00715950
https://hal-insu.archives-ouvertes.fr/insu-00715950/document
https://hal-insu.archives-ouvertes.fr/insu-00715950/file/Jolivet-Aegean_Tectonophysics-2012.pdf
DOI : 10.1016/j.tecto.2012.06.011

Éditeur(s) : HAL CCSD Oxford University Press (OUP)
Résumé : International audience
Tilt fluctuations can potentially reflect the response of hydrosystems to important rainfall. In this context, long baseline tiltmeters have been installed in an underground tunnel penetrating the Fontaine de Vaucluse karst to study the medium deformation related to solicitations exerted by water infiltrating the hydrosystem. The instruments monitor the tilt as well as its spatial variation. Northward tilts reaching a 1 μrad amplitude are observed consecutively to rainfalls. The tilt amplitude is highly correlated with the Fontaine de Vaucluse outlet flow fluctuations. The measured tilt signal is also relatively homogeneous over a 150 m length. Different types of structure likely to produce such observations are tested in order to identify their location with respect to the tiltmeters, their dimension as well as the amount of water level variation in the structure. Following rainfalls, the infiltration of water modifies the pore pressure, inducing a medium deformation. The hypothesis of an homogeneous surface loading on the Vaucluse plateau is first refuted since the related tilt is much lower than the one measured. The water supplied by rainfalls has to accumulate in discontinuities in order to generate a higher tilt. So, the deformation related to a pressure exerted on a fracture filled by water is assessed. A first study reveals the interest of the tilt homogeneity information that constrains strongly the fracture properties. Thus, the fracture must be located at a distance more than a few hundreds metres from the tiltmeters in order to produce a tilt homogeneous in space. If the fracture is initially dry, it must also be filled on a height higher than 150 m consecutive to a rainfall in order to generate a tilt amplitude in the same magnitude as the one measured. Then, we explore the influence of water level variations on the tilt produced by a fracture located at the interface between the saturated and unsaturated zones, which are thereby permanently flooded. Since several parameters of that model satisfactorily explain the field observations, we discuss how simultaneous geodetical observations could provide complementary information that would further constrain the geometry of the structure at the origin of the medium deformation.
ISSN: 0956-540X

insu-01408482
https://hal-insu.archives-ouvertes.fr/insu-01408482
https://hal-insu.archives-ouvertes.fr/insu-01408482/document
https://hal-insu.archives-ouvertes.fr/insu-01408482/file/Lesparre-2017.pdf
DOI : 10.1093/gji/ggw446

Éditeur(s) : HAL CCSD Elsevier
Résumé : International audience
Benzo[a]pyrene (B[a]P), the prototype molecule of polycyclic aromatic hydrocarbons, exhibits genotoxic and carcinogenic effects, which has led the International Agency for Research on Cancer to recognize it as a human carcinogen. Besides the well-known apoptotic signals triggered by B[a]P, survival signals have also been suggested to occur, both signals likely involved in cancer promotion. Our previous work showed that B[a]P induced an hyperpolarization of mitochondrial membrane potential (ΔΨm) in rat hepatic epithelial F258 cells. Elevated ΔΨm plays a role in tumor development and progression, and nitric oxide (NO) has been suggested to be responsible for increases in ΔΨm. The present study therefore aimed at evaluating the impact of B[a]P on NO level in F258 cells, and at testing the putative role for NO as a survival signal, notably in link with ΔΨm. Our data demonstrated that B[a]P exposure resulted in an NO production which was dependent upon the activation of the inducible NO synthase. This enzyme activation involved AhR and possibly p53 activation. Preventing NO production not only increased B[a]P-induced cell death but also blocked mitochondrial hyperpolarization. This therefore points to a role for NO as a survival signal upon B[a]P exposure, possibly targeting ΔΨm
ISSN: 0887-2333

hal-01163757
https://hal-univ-rennes1.archives-ouvertes.fr/hal-01163757
https://hal-univ-rennes1.archives-ouvertes.fr/hal-01163757/document
https://hal-univ-rennes1.archives-ouvertes.fr/hal-01163757/file/Benzo%5Ba%5Dpyrene-induced%20nitric.pdf
DOI : 10.1016/j.tiv.2015.06.010

Éditeur(s) : HAL CCSD Météo et Climat
Résumé : International audience
Les risques hydrométéorologiques sont au coeur du programme de recherche HyMeX sur le cycle de l'eau en Méditerranée. HyMeX a pour ambition d'améliorer leur prévision, de mieux connaître leur variabilité à l'échelle pluriannuelle et de renseigner sur leur évolution, dans le contexte du changement climatique. À cette fin, deux campagnes de mesures dédiées à ces événements extrêmes (SOP1 : pluies intenses et crues rapides ; SOP2 : vents forts et formation d'eaux denses) sont organisées en Méditerranée nord-occidentale, entre septembre 2012 et mars 2013.
ISSN: 0026-1181

hal-00829760
https://hal.archives-ouvertes.fr/hal-00829760
DOI : 10.4267/2042/48793

Éditeur(s) : HAL CCSD International Frequency Sensor Association (IFSA)
Résumé : International audience
This paper introduces an approach illustrating how the Open Geospatial Consortium (OGC) Sensor Web Enablement (SWE) framework can be used in order to build a franco-lebanese observatory. We present the practical application of SWE services as a source of real-time observation data and the associated technical architecture for making near real-time observations available to end users on the Web. We discuss the question of crossing sensor data with other data sources, e.g., data provided by human observations. We illustrate our approach by describing the methodology to integrate a first illustration case to monitor snow weather stations in the Lebanese Mountains.
ISSN: 1726-5479

lirmm-01381092
https://hal-lirmm.ccsd.cnrs.fr/lirmm-01381092

Éditeur(s) : HAL CCSD Société nationale de protection de la nature (France)
Résumé : International audience
Les plantes qui présentent des structures anatomiques permettant de retenir de l'eau en permanence sont assez répandues en milieu tropical. Si beaucoup sont maintenant cultivées pour être vendues en jardineries, faisant le bonheur des amateurs, elles forment en milieu naturel des écosystèmes aquatiques encore très peu étudiés et renferment une biodiversité que l'on est loin d'avoir recensée. En Amérique centrale et du Sud, les broméliacées-citerne, qui représentent les plus nombreuses et les plus diversifiées de ces " plantes-mares ", permettent à des organismes très variés d'accomplir leur cycle de vie
ISSN: 0011-0477

hal-00849049
https://hal.archives-ouvertes.fr/hal-00849049
https://hal.archives-ouvertes.fr/hal-00849049/document
https://hal.archives-ouvertes.fr/hal-00849049/file/Carrias2013_Des_plantes.pdf

Éditeur(s) : HAL CCSD Landes Bioscience
Résumé : International audience
In 2008 we published the first set of guidelines for standardizing research in autophagy. Since then, research on this topic has continued to accelerate, and many new scientists have entered the field. Our knowledge base and relevant new technologies have also been expanding. Accordingly, it is important to update these guidelines for monitoring autophagy in different organisms. Various reviews have described the range of assays that have been used for this purpose. Nevertheless, there continues to be confusion regarding acceptable methods to measure autophagy, especially in multicellular eukaryotes. A key point that needs to be emphasized is that there is a difference between measurements that monitor the numbers or volume of autophagic elements (e.g., autophagosomes or autolysosomes) at any stage of the autophagic process vs. those that measure flux through the autophagy pathway (i.e., the complete process); thus, a block in macroautophagy that results in autophagosome accumulation needs to be differentiated from stimuli that result in increased autophagic activity, defined as increased autophagy induction coupled with increased delivery to, and degradation within, lysosomes (in most higher eukaryotes and some protists such as Dictyostelium) or the vacuole (in plants and fungi). In other words, it is especially important that investigators new to the field understand that the appearance of more autophagosomes does not necessarily equate with more autophagy. In fact, in many cases, autophagosomes accumulate because of a block in trafficking to lysosomes without a concomitant change in autophagosome biogenesis, whereas an increase in autolysosomes may reflect a reduction in degradative activity. Here, we present a set of guidelines for the selection and interpretation of methods for use by investigators who aim to examine macroautophagy and related processes, as well as for reviewers who need to provide realistic and reasonable critiques of papers that are focused on these processes. These guidelines are not meant to be a formulaic set of rules, because the appropriate assays depend in part on the question being asked and the system being used. In addition, we emphasize that no individual assay is guaranteed to be the most appropriate one in every situation, and we strongly recommend the use of multiple assays to monitor autophagy. In these guidelines, we consider these various methods of assessing autophagy and what information can, or cannot, be obtained from them. Finally, by discussing the merits and limits of particular autophagy assays, we hope to encourage technical innovation in the field.
Autophagy

hal-00735751
https://hal.archives-ouvertes.fr/hal-00735751
PUBMED : 22966490

Éditeur(s) : HAL CCSD
Résumé : International audience
Conférence MISTRALS PAELOMEX
Montpellier, France

hal-01683547
https://hal-univ-tlse2.archives-ouvertes.fr/hal-01683547