Éditeur(s) : HAL CCSD Elsevier
Résumé : International audience
A series of two-stage torsion experiments on Carrara marble were conducted to constrain the influence of known prior deformation on rheological response and microstructural evolution. Comparison with previous experimental data on Carrara marble flow and fabric evolution during single-stage torsion deformation experiments provided direct insights into the significance of initial deformation at various conditions. Our experiments were conducted at 727 degrees C temperature and 300 MPa confining pressure, while maintaining a constant strain rate of 3 x 10(-4) s(-1) on the periphery of the cylindrical samples. Under these conditions, the marble is known to deform in power-law (n = 6-10) ductile flow. All torsion experiments were performed with a Paterson type gas-medium testing machine equipped with a torsion actuator module.;Prior (D1) and subsequent (D2) deformation are accomplished by two torsion experiments in sequence on same sample segments. The effect of D1 strain history is investigated during D2 by applying counter-clockwise torsion to a sandwich sample consisting of three segments with different D1 rotation sense. D2 samples experienced continued, first and reversed shearing deformation in top, centre and bottom segments, respectively. D2 bulk strain was chosen equal to D1 strain in top and bottom segments.;D1 experiments followed the typical single-stage deformation behaviour of Carrara marble under the applied experimental conditions. Yielding was followed by strain hardening until a peak stress was reached at a shear strain around 1, after which work softening occurred. Weakening gradually evolved into a constant stress regime. During hardening, a shear microstructure and crystallographic preferred orientation (CPO) developed. Afterwards the volume fraction of smaller dynamic recrystallised grains increased continuously, resulting in a recrystallisation microstructure and CPO at shear strains of 5 and higher. The new D2 experiments displayed a strain variation between homogeneously deformed sample segments that increased with increasing D1 strain. The stress-strain behaviour of the D2 bulk sandwich samples showed less pronounced work-hardening and -softening when compared with single-stage deformation experiments. Furthermore, constant flow stress was attained at increasingly lower strain with increasing D1 strain. In most D2 segments, fabric development is equivalent to single-stage experiments at corresponding absolute strain. The fabrics differed markedly from those in single-stage experiments in the case of reversed two-stage deformation at moderate strain (D1 shear strain 1 and 2.6). Experiments show that grain shape by shearing of relict grains is defined by finite strain and thus affected by strain reversal. Recrystallisation is controlled by absolute strain and not influenced by strain reversal.
ISSN: 0040-1951

hal-00617686
https://hal.archives-ouvertes.fr/hal-00617686
DOI : 10.1016/j.tecto.2010.09.029

Éditeur(s) : HAL CCSD American Geophysical Union
Résumé : International audience
Strain and strain rate partitioning in partially molten rocks are two of the important mechanisms that govern the process of coupling and/or decoupling of the partially molten lithosphere. Consequently, the proportion of partial melt and crystals and their network in partially molten rocks influence the degree of the partitioning along with the bulk rheology of the system. This study explores the possible role of finite strain and strain rate on the rate and volume of partial melting and crystallization in a metapelitic system undergoing deformation. Cylinders of synthetic quartz-muscovite aggregate (7: 3 volume ratio) were deformed in torsion at 750 C, 300 MPa and constant shear strain rate ((gamma) over dot = 3 x 10(-4) s(-1)) for finite shear strains (gamma) 1-15. The deformed samples were studied along the longitudinal tangential ( LT) and axial ( LA) sections to obtain the data along a range of strain rates for a given finite strain and vice versa. The results showed that deformation plays an important role on the kinetics of partial melting and crystallization. With increasing strain rate, amount and rate of crystallization comprise the volumetrically dominant process compared to partial melting at a given finite strain. In contrast, when the strain rate is constant, partial melting is the dominant process over crystallization up to moderate strain (gamma < 5). The dominant process reverses at higher strain, and the system shows more crystallization than partial melting. Application of the experimental data to geological systems implies that for metapelites a significant amount (similar to 20%) of partial melt can generate at high strain rate and moderate strain (gamma similar to 7), but at high strain (gamma = 15) the system is melt depleted. Under such conditions, decoupling should take place in brittle-ductile mode. On the other hand, rocks undergoing deformation with low strain rates and strain (gamma < 3) contain more than 25% partial melt, which can act as a major decoupling agent by localizing ductile shear zones.
ISSN: 0148-0227

hal-00617714
https://hal.archives-ouvertes.fr/hal-00617714
DOI : 10.1029/2010JB007865

Éditeur(s) : HAL CCSD American Society for Microbiology
Résumé : International audience
Under physiological conditions, the liver sinusoidal endothelial cells (LSECs) mediate hepatic immune tolerance toward self or foreign antigens through constitutive expression of anti-inflammatory mediators. However, upon viral infection or Toll-like receptor 2 (TLR2) activation, LSECs can achieve proinflammatory functions, but their role in hepatic inflammation during acute viral hepatitis is unknown. Using the highly virulent mouse hepatitis virus type 3 (MHV3) and the attenuated variants 51.6-MHV3 and YAC-MHV3, exhibiting lower tropism for LSECs, we investigated in vivo and in vitro the consequence of LSEC infection on their proinflammatory profiles and the aggravation of acute hepatitis process. In vivo infection with virulent MHV3, in comparison to attenuated strains, resulted in fulminant hepatitis associated with higher hepatic viral load, tissue necrosis, and levels of inflammatory mediators and earlier recruitment of inflammatory cells. Such hepatic inflammatory disorders correlated with disturbed production of interleukin-10 (IL-10) and vascular factors by LSECs. We next showed in vitro that infection of LSECs by the virulent MHV3 strain altered their production of anti-inflammatory cytokines and promoted higher release of proinflammatory and procoagulant factors and earlier cell damage than infection by attenuated strains. This higher replication and proinflammatory activation in LSECs by the virulent MHV3 strain was associated with a specific activation of TLR2 signaling by the virus. We provide evidence that TLR2 activation of LSCEs by MHV3 is an aggravating factor of hepatic inflammation and correlates with the severity of hepatitis. Taken together, these results indicate that preservation of the immunotolerant properties of LSECs during acute viral hepatitis is imperative in order to limit hepatic inflammation and damage. IMPORTANCE Viral hepatitis B and C infections are serious health problems affecting over 350 million and 170 million people worldwide, respectively. It has been suggested that a balance between protection and liver damage mediated by the host's immune response during the acute phase of infection would be determinant in hepatitis outcome. Thus, it appears crucial to identify the factors that predispose in exacerbating liver inflammation to limit hepatocyte injury. Liver sinusoidal endothelial cells (LSECs) can express both anti- and proinflammatory functions, but their role in acute viral hepatitis has never been investigated. Using mouse hepatitis virus (MHV) infections as animal models of viral hepatitis, we report for the first time that in vitro and in vivo infection of LSECs by the pathogenic MHV3 serotype leads to a reversion of their intrinsic anti-inflammatory phenotype toward a proinflammatory profile as well to as disorders in vascular factors, correlating with the severity of hepatitis. These results highlight a new virus-promoted mechanism of exacerbation of liver inflammatory response during acute hepatitis.
ISSN: 0022-538X

hal-01390974
https://hal-univ-rennes1.archives-ouvertes.fr/hal-01390974
https://hal-univ-rennes1.archives-ouvertes.fr/hal-01390974/document
https://hal-univ-rennes1.archives-ouvertes.fr/hal-01390974/file/Mouse%20Hepatitis%20Virus%20Infection_accepted.pdf
DOI : 10.1128/JVI.01069-16

Éditeur(s) : HAL CCSD American Association of Petroleum Geologists
Résumé : International audience
We describe the structure, microstructure, and petrophysical properties of fault rocks from two normal fault zones formed in low-porosity turbiditic arkosic sandstones, in deep diagenesis conditions similar to those of deeply buried reservoirs. These fault rocks are characterized by a foliated fabric and quartz-calcite sealed veins, which formation resulted from the combination of the (1) pressure solution of quartz, (2) intense fracturing sealed by quartz and calcite cements, and (3) neoformation of synkinematic white micas derived from the alteration of feldspars and chlorite. Fluid inclusion microthermometry in quartz and calcite cements demonstrates fault activity at temperatures of 195degC to 268degC. Permeability measurements on plugs oriented parallel with the principal axes of the finite strain ellipsoid show that the Y axis (parallel with the foliation and veins) is the direction of highest permeability in the foliated sandstone (10-2 md for Y against 10-3 md for X, Z, and the protolith, measured at a confining pressure of 20 bars). Microstructural observations document the localization of the preferential fluid path between the phyllosilicate particles forming the foliation. Hence, the direction of highest permeability in these fault rocks would be parallel with the fault and subhorizontal, that is, perpendicular to the slickenlines representing the local slip direction on the fault surface. We suggest that a similar relationship between kinematic markers and fault rock permeability anisotropy may be found in other fault zone types (reverse or strike-slip) affecting feldspar-rich lithologies in deep diagenesis conditions.
ISSN: 0149-1423

hal-00903428
https://hal.archives-ouvertes.fr/hal-00903428
DOI : 10.1306/03071312127

Éditeur(s) : HAL CCSD Elsevier
Résumé : International audience
The analysis of three cataclastic band sets from Provence (France) reveals that the band density, their conjugate angles, their ratio of shear displacement to compaction, and the amount of cataclasis within the bands differ and can be expressed as functions of tectonic setting and petrophysical properties. We identify (1) a dense and closely spaced network of shear-enhanced (reverse) compaction bands; (2) a regularly spaced less dense network of reverse compactional shear bands; and (3) a localized network of normal shear bands. The field data show that strain localization is favored in an extensional regime and is characterized by shear bands with a large shear to compaction ratio and a small conjugate band angle. In contrast, distributed strain is favored in a contractional regime and is characterized by compactional bands with a low ratio of shear to compaction and a large conjugate band angle. To explain the mechanical origin of this strain localization, we quantify the yield strength and the stress evolution in extensional and contractional regimes in a frictional porous granular material. We propose a model of strain localization in porous sands as a function of tectonic stresses, burial depth, material properties, strain hardening and fluid pressure. Our model suggests that stress reduction, inherent to extensional regime, favors strain localization as shear bands, whereas stress increase during contraction favors development of compactional bands.
ISSN: 0191-8141

hal-00856921
https://hal.archives-ouvertes.fr/hal-00856921
DOI : 10.1016/j.jsg.2012.11.011

Éditeur(s) : HAL CCSD Mary Ann Liebert
Résumé : International audience
Little information is available on the molecular epidemiologic profile of HIV-1 in French Guiana, the French department with the highest HIV/AIDS incidence. To follow the evolution of HIV-1 diversity, we carried out a molecular analysis of HIV-1 isolates from 305 treatment-naive patients between 2006 and 2012. Protease and reverse-transcriptase sequences were obtained for subtype characterization, polymorphism analysis, and identification of drug resistance mutations. Of 305 HIV-1 strains, 95.1% were subtype B viruses. The overall prevalence of transmitted drug-resistance mutations (TDRMs) was 4.6% (14/305), ranging from 1.9% to 7.1% depending on the year. This study shows a low level of HIV-1 genetic diversity and a moderate prevalence of TDRMs with no evidence of an increasing trend over the study period. Nevertheless, the strong genetic polymorphism observed on both genes may be of concern for long-term treatment of people living with HIV-1 and thus deserves continuous monitoring.
ISSN: 0889-2229

pasteur-01583613
https://hal-riip.archives-ouvertes.fr/pasteur-01583613
https://hal-riip.archives-ouvertes.fr/pasteur-01583613/document
https://hal-riip.archives-ouvertes.fr/pasteur-01583613/file/2016%20Darcissac%20et%20al.%20ARHR.pdf
DOI : 10.1089/aid.2016.0048

Éditeur(s) : HAL CCSD Elsevier
Résumé : International audience
Cyanobacteria are ubiquitous microorganisms considered as important contributors to the formation of Earth's atmosphere and nitrogen fixation. However, they are also frequently associated with toxic blooms. Indeed, the wide range of hepatotoxins, neurotoxins and dermatotoxins synthesized by these bacteria is a growing environmental and public health concern. This paper provides a state of the art on the occurrence and management of harmful cyanobacterial blooms in surface and drinking water, including economic impacts and research needs. Cyanobacterial blooms usually occur according to a combination of environmental factors e.g., nutrient concentration, water temperature, light intensity, salinity, water movement, stagnation and residence time, as well as several other variables. These environmental variables, in turn, have promoted the evolution and biosynthesis of strain-specific, gene-controlled metabolites (cyanotoxins) that are often harmful to aquatic and terrestrial life, including humans. Cyanotoxins are primarily produced intracellularly during the exponential growth phase. Release of toxins into water can occur during cell death or senescence but can also be due to evolutionary-derived or environmentally-mediated circumstances such as allelopathy or relatively sudden nutrient limitation. Consequently, when cyanobacterial blooms occur in drinking water resources, treatment has to remove both cyanobacteria (avoiding cell lysis and subsequent toxin release) and aqueous cyanotoxins previously released. Cells are usually removed with limited lysis by physical processes such as clarification or membrane filtration. However, aqueous toxins are usually removed by both physical retention, through adsorption on activated carbon or reverse osmosis, and chemical oxidation, through ozonation or chlorination. While the efficient oxidation of the more common cyanotoxins (microcystin, cylindrospermopsin, anatoxin and saxitoxin) has been extensively reported, the chemical and toxicological characterization of their by-products requires further investigation. In addition, future research should also investigate the removal of poorly considered cyanotoxins (β-methylamino-alanine, lyngbyatoxin or aplysiatoxin) as well as the economic impact of blooms.
Environment International

hal-00875835
https://hal-univ-rennes1.archives-ouvertes.fr/hal-00875835
https://hal-univ-rennes1.archives-ouvertes.fr/hal-00875835/document
DOI : 10.1016/j.envint.2013.06.013
PUBMED : 23892224

Éditeur(s) : HAL CCSD
Résumé : This study is designed to improve the understanding of structural, mechanical and petrophysical characteristics of deformation bands in porous sandstone such as their potential influence on fluid storage or migration in reservoir setting. Structural and petrophysical analysis of band networks from Provence show that permeability of band is mainly controlled by the degree of cataclasis and diagenetic processes at depth. Map-scale analysis of band networks underlines the major influence of tectonic regime on band distribution, organization and permeability. Band associated to normal-fault regime (cataclasis and low-permeability) are located around map-sclae-fault whereas band associated to thurst-fault regime (moderate cataclasis and permeability) are pervasively distributed in all the area. Reverse-sense band are cataclastic and low-permeabiilty around the Roquemaure thrust, which show the potential role of large-scale fault on structural and petrophysical characteristics of bands. This analysis also shows the influence of fault propagation on formation of low permeability catacastic bands. This analysis suggests also granulometry as factor controlling organization of band networks.A mechanical analysis of deformation bands is realized based on results from Provence. Yield envelopes of these materials are calculated from analytical solution. Triaxial tests done on sandstone (L'Etang quarry, Orange) confirm the shape of yield envelope theoretically calculated. Triaxial tests done on the poorly-lithified sands (Boncavaï quarry, Uchaux) show grain size, sorting and packing influencing yield envelope and cataclastic band localization at shallow burial depth. Stress paths are calculated for burial and tectonic event (extension and contraction) using the relationship between main stress and secondary stresses (K0) calibrated from well data. These different results are integrated into a model which allows to estimate type and structural characteristics of bands susceptible to form for a known geological setting.A structural, mechanical and petrophysical model is established form these results. This model is calibrated by permeability data from deformation bands of Provence and results from the mechanical model of strain localization. This model is also confronted to a synthesis of permeability data from literature, which confirms the influence of the different controlling parameters integrated to the model. This model is applied for two sites of uranium exploration/exploitation in porous sandstone reservoirs and validated by field observations.
Cette étude est destinée à améliorer la compréhension des caractéristiques structurales, pétrophysiques et mécaniques des bandes de déformation dans les grès poreux ainsi que de leur rôle potentiel sur la migration ou le piégeage des fluides en milieu réservoir. L'analyse structurale et pétrophysique des réseaux de bandes de Provence montre que leur perméabilité découle principalement de l'intensité de la cataclase dans leur microstructure et des processus diagénétiques qui peuvent s'y localiser avec la profondeur. L'analyse régionale de ces réseaux de bandes souligne l'influence majeure du régime tectonique sur leur distribution, leur organisation et leur perméabilité. Les bandes associées au régime normal (cataclactiques et peu perméables) sont localisées autour des failles cartographiques, tandis que les bandes associées au régime inverse (modérement cataclastiques et perméables) sont distribuées dans toute la région. Les bandes inverses sont fortement cataclastiques et imperméables uniquement autour du chevauchement de Roquemaure ce qui montre le rôle potentiel de la présence de grande faille sur les caractéristiques structurales et pétrophysiques de ces bandes. D'autre part, cette analyse suggère que la granulométrie de l'encaissant peut influencer l'initiation et l'organisation des réseaux. L'analyse mécanique des bandes de déformation a ensuite été réalisée à partir des résultats obtenus en Provence. L'enveloppe de plasticité de ces matériaux est calculée à l'aide d'une solution analytique. Les essais triaxiaux menés sur le grès de la carrière de l'Etang (orange) confirment la forme de ces enveloppes estimées théoriquement. Les essais réalisés sur les sables de Boncavaï (Uchaux) montrent que la taille des grains, le tri et de la compaction dans ces matériaux peu lithifiés influent sur leurs enveloppes de plasticité et la localisation des bandes cataclastiques à faibles profondeurs. Les trajets de contraintes sont estimés pour les phases d'enfouissement et de chargement tectonique (extension et compression) à partir de la relation entre contrainte principale et contraintes secondaires (K0), calibrée à partir de données de forage. Ces résultats sont intégrés à un modèle permettant d'estimer la nature et les caractéristiques structurales des bandes susceptibles de se former dans un contexte géologique donné. Un modèle structural, mécanique et pétrophysique a été établir à partir de ces différents résultats. Ce modèle est calibré à partir des données de perméabilité mesurées sur les bandes de Provence et des résultats du modèle mécanique de localisation cité précédemment. Ce modèle a ensuite été confronté à une synthèse des données de perméabilité disponibles dans la littérature et confirme l'influence des différents paramètres de contrôle pris en compte dans le modèle. Ce modèle a enfin pu être appliqué sur deux sites d'explorations/exploitations d'uranium en réservoir gréseux poreux et validé par les résultats obtenus, en concordance avec les observations de terrain.
https://tel.archives-ouvertes.fr/tel-01001389

NNT : 2013MON20067
tel-01001389
https://tel.archives-ouvertes.fr/tel-01001389
https://tel.archives-ouvertes.fr/tel-01001389/document
https://tel.archives-ouvertes.fr/tel-01001389/file/36072_BALLAS_2013_archivage-1_cor.pdf