Éditeur(s) : Pub. by J.B. Smith & Co. Pub. by J.B. Smith & Co. ( Philadelphia (No. 610 Chestnut Street) )
Résumé : (Statement of Responsibility) by Daniel Defoe ; two volumes in one.
Gilt, decorative spine with title: Robinson Crusoe.
Undated reissue of Lovett, R.W. Robinson Crusoe, 458 and NUC pre- 1956, 0118338 (v. 136, p. 598) both of which are dated 1859.
Col. front.: Sinclair's Lith. Probably Thomas S. Sinclair, a lithographer active in Philadelphia from the 1840s.
All pages have fancy ruled frame.
Parts I and II of Robinson Crusoe. Part II originally published under title: Farther adventures of Robinson Crusoe.
(Funding) Preservation and Access for American and British Children's Literature, 1870-1889 (NEH PA-50860-00).
United States -- Pennsylvania -- Philadelphia

Droits : This item is presumed to be in the public domain. The University of Florida George A. Smathers Libraries respect the intellectual property rights of others and do not claim any copyright interest in this item. Users of this work have responsibility for determining copyright status prior to reusing, publishing or reproducing this item for purposes other than what is allowed by fair use or other copyright exemptions. Any reuse of this item in excess of fair use or other copyright exemptions may require permission of the copyright holder. The Smathers Libraries would like to learn more about this item and invite individuals or organizations to contact The Department of Special and Area Studies Collections (special@uflib.ufl.edu) with any additional information they can provide.
27866802

Éditeur(s) : McLoughlin Brother's, Publishers McLoughlin Brother's, Publishers ( New York )
Résumé : (Statement of Responsibility) by Mary Godolphin ; with colored illustrations.
An adaptation of Thomas Day's History of Sandford and Merton.
Date of publication from inscription.
Cover title: Sanford [sic] and Merton.
Text in double columns.
(Funding) Preservation and Access for American and British Children's Literature, 1870-1889 (NEH PA-50860-00).
United States -- New York -- New York

Droits : This item is presumed to be in the public domain. The University of Florida George A. Smathers Libraries respect the intellectual property rights of others and do not claim any copyright interest in this item. Users of this work have responsibility for determining copyright status prior to reusing, publishing or reproducing this item for purposes other than what is allowed by fair use or other copyright exemptions. Any reuse of this item in excess of fair use or other copyright exemptions may require permission of the copyright holder. The Smathers Libraries would like to learn more about this item and invite individuals or organizations to contact The Department of Special and Area Studies Collections (special@uflib.ufl.edu) with any additional information they can provide.
002248820
ALK0545
174964989

Éditeur(s) : El Ministerio El Ministerio ( Caracas )
Résumé : (Additional Physical Form) Also issued on microfilm from Library of Congress Preservation Microfilming Program.
(Numbering Peculiarities) Report year ends in April.
(Numbering Peculiarities) No report published in 1906.
(Issuing Body) 18??-18?? issued by Ministerio de lo Interior y Justicia; 18??- by Ministerio de Relaciones Interiores.
Part of illustrative material is folded.
The dates given are those on the title-pages, corresponding to the date of transmittal of reports, which are usually for the preceding fiscal years.
Venezuela
Venezuela
Venezuela
Venezuela

01643059
45040626
ocm01643059
http://ufdc.ufl.edu/AA00016875/00001

Éditeur(s) : Bickers & Son Bickers & Son ( London (1 Leicester Square) )
Résumé : Spine title: Robinson Crusoe; caption title: Adventures of Robinson Crusoe.
Publisher's catalog dated Oct., 1881, 16 p. at end. Catalog printed by Chiswick Press:--C. Whittingham and Co., Tooks Court, Chancery Lane.
Some ill. signed H. Anelay; printed by W. Dickes.
"Ballantyne Press, Ballantyne, Hanson and Co., Edinburgh and London"--Verso of half-title p.
Parts I and II of Robinson Crusoe.
Bookbinder's label, p. [3] of cover: Bound by Leighton Son and Hodge.
(Funding) Preservation and Access for American and British Children's Literature, 1870-1889 (NEH PA-50860-00).
(Statement of Responsibility) by Daniel Defoe ; with a memoir of the author and coloured illustrations.
England -- London
Scotland -- Edinburgh

Droits : This item is presumed to be in the public domain. The University of Florida George A. Smathers Libraries respect the intellectual property rights of others and do not claim any copyright interest in this item. Users of this work have responsibility for determining copyright status prior to reusing, publishing or reproducing this item for purposes other than what is allowed by fair use or other copyright exemptions. Any reuse of this item in excess of fair use or other copyright exemptions may require permission of the copyright holder. The Smathers Libraries would like to learn more about this item and invite individuals or organizations to contact The Department of Special and Area Studies Collections (special@uflib.ufl.edu) with any additional information they can provide.
001778777
30304572
AJK2066

Éditeur(s) : El Ministerio El Ministerio ( Caracas )
Résumé : (Additional Physical Form) Also issued on microfilm from Library of Congress Preservation Microfilming Program.
(Numbering Peculiarities) Report year ends in April.
(Numbering Peculiarities) No report published in 1906.
(Issuing Body) 18??-18?? issued by Ministerio de lo Interior y Justicia; 18??- by Ministerio de Relaciones Interiores.
Part of illustrative material is folded.
The dates given are those on the title-pages, corresponding to the date of transmittal of reports, which are usually for the preceding fiscal years.
Venezuela
Venezuela
Venezuela
Venezuela

01643059
45040626
ocm01643059
http://ufdc.ufl.edu/AA00016875/00001

Éditeur(s) : El Ministerio El Ministerio ( Caracas )
Résumé : (Additional Physical Form) Also issued on microfilm from Library of Congress Preservation Microfilming Program.
(Numbering Peculiarities) Report year ends in April.
(Numbering Peculiarities) No report published in 1906.
(Issuing Body) 18??-18?? issued by Ministerio de lo Interior y Justicia; 18??- by Ministerio de Relaciones Interiores.
Part of illustrative material is folded.
The dates given are those on the title-pages, corresponding to the date of transmittal of reports, which are usually for the preceding fiscal years.
Venezuela
Venezuela
Venezuela
Venezuela

01643059
45040626
ocm01643059
http://ufdc.ufl.edu/AA00016875/00001

Éditeur(s) : Loomis and Peck Loomis and Peck ( Philadelphia )
Résumé : (Citation/Reference) Lovett, R.W. Robinson Crusoe,
(Citation/Reference) NUC Pre-1956
Spine title: Robinson Crusoe.
Date on cover: 1846.
"B.M. Dusenbery, Stereotyper."--T.p. verso.
Publisher's advertisement on p. 4 of cover.
Part I of Robinson Crusoe, retold.
(Funding) Preservation and Access for American and British Children's Literature, 1870-1889 (NEH PA-50860-00).
United States -- Pennsylvania -- Philadelphia

Droits : This item is presumed to be in the public domain. The University of Florida George A. Smathers Libraries respect the intellectual property rights of others and do not claim any copyright interest in this item. Users of this work have responsibility for determining copyright status prior to reusing, publishing or reproducing this item for purposes other than what is allowed by fair use or other copyright exemptions. Any reuse of this item in excess of fair use or other copyright exemptions may require permission of the copyright holder. The Smathers Libraries would like to learn more about this item and invite individuals or organizations to contact The Department of Special and Area Studies Collections (special@uflib.ufl.edu) with any additional information they can provide.
27020839

Éditeur(s) : El Ministerio El Ministerio ( Caracas )
Résumé : (Additional Physical Form) Also issued on microfilm from Library of Congress Preservation Microfilming Program.
(Numbering Peculiarities) Report year ends in April.
(Numbering Peculiarities) No report published in 1906.
(Issuing Body) 18??-18?? issued by Ministerio de lo Interior y Justicia; 18??- by Ministerio de Relaciones Interiores.
Part of illustrative material is folded.
The dates given are those on the title-pages, corresponding to the date of transmittal of reports, which are usually for the preceding fiscal years.
Venezuela
Venezuela
Venezuela
Venezuela

01643059
45040626
ocm01643059
http://ufdc.ufl.edu/AA00016875/00001

Éditeur(s) : El Ministerio El Ministerio ( Caracas )
Résumé : (Additional Physical Form) Also issued on microfilm from Library of Congress Preservation Microfilming Program.
(Numbering Peculiarities) Report year ends in April.
(Numbering Peculiarities) No report published in 1906.
(Issuing Body) 18??-18?? issued by Ministerio de lo Interior y Justicia; 18??- by Ministerio de Relaciones Interiores.
Part of illustrative material is folded.
The dates given are those on the title-pages, corresponding to the date of transmittal of reports, which are usually for the preceding fiscal years.
Venezuela
Venezuela
Venezuela
Venezuela

01643059
45040626
ocm01643059
http://ufdc.ufl.edu/AA00016875/00001

Éditeur(s) : El Ministerio El Ministerio ( Caracas )
Résumé : (Additional Physical Form) Also issued on microfilm from Library of Congress Preservation Microfilming Program.
(Numbering Peculiarities) Report year ends in April.
(Numbering Peculiarities) No report published in 1906.
(Issuing Body) 18??-18?? issued by Ministerio de lo Interior y Justicia; 18??- by Ministerio de Relaciones Interiores.
Part of illustrative material is folded.
The dates given are those on the title-pages, corresponding to the date of transmittal of reports, which are usually for the preceding fiscal years.
Venezuela
Venezuela
Venezuela
Venezuela

01643059
45040626
ocm01643059
http://ufdc.ufl.edu/AA00016875/00001

Éditeur(s) : El Ministerio El Ministerio ( Caracas )
Résumé : (Additional Physical Form) Also issued on microfilm from Library of Congress Preservation Microfilming Program.
(Numbering Peculiarities) Report year ends in April.
(Numbering Peculiarities) No report published in 1906.
(Issuing Body) 18??-18?? issued by Ministerio de lo Interior y Justicia; 18??- by Ministerio de Relaciones Interiores.
Part of illustrative material is folded.
The dates given are those on the title-pages, corresponding to the date of transmittal of reports, which are usually for the preceding fiscal years.
Venezuela
Venezuela
Venezuela
Venezuela

01643059
45040626
ocm01643059
http://ufdc.ufl.edu/AA00016875/00001

Éditeur(s) : HAL CCSD Elsevier
Résumé : Une approche par datations multiples a été menée sur les dépôts de sédiments de crue retrouvés dans les gorges du Gardon (Languedoc). Le plomb-210, le césium-137 et l’analyse géochimique des sédiments contaminés par les mines ont été utilisés pour reconstruire l’histoire des dépôts de crue. Cette approche a été combinée avec l’historique des crues du Gardon depuis 1890 pour affiner l’approche géochronologique. Au niveau de la cavité GG et de la terrasse GE les analyses ont permis d’illustrer les mécanismes d’érosion-redéposition pouvant perturber les séquences de sédiments. Les enregistrements des dépôts de sédiments de crue sont complets sur la terrasse GE située à 10 m au-dessus du lit mineur à partir de 1958, et incomplets auparavant. Sur la période 1880-1958, 25 crues ont eu un débit suffisant (>1450 m3/s) pour recouvrir la terrasse, alors que l’on recense seulement 5 à 6 crues depuis 1958 (pour un débit de submersion supérieur à 1700 m3/s). L’augmentation progressive du débit de submersion et la fréquence moindre d’inondation de la terrasse ont pu permettre sur la période récente la stabilisation de la couche de surface par la végétation et limiter l’érosion lors de crues importantes. Les enregistrements semblent complets dans la cavité GG située à 15 m au-dessus du lit mineur. La fréquence moindre de submersion à ce niveau plus élevé peut expliquer une meilleure stabilisation des dépôts de crue, rendus moins vulnérables à l’érosion. La datation au radiocarbone a été testée même si elle ne permet pas de dater avec précision les dépôts de crue des derniers siècles. Sur la terrasse GE, 80% des dates au carbone 14 sur charbon de bois sont beaucoup trop vieilles par rapport aux autres techniques de datation, ce qui peut s’expliquer par une remobilisation/déstockage de charbons de bois anciens. Les datations radiocarbone sur graines donnent de meilleurs résultats, car issues d’une production annuelle, plus facilement décomposable et moins sujette à des dépôts intermédiaires. Au final, il apparaît que la technique de datation par radiocarbone sur des charbons de bois retrouvés dans les séquences sédimentaires n’est pas fiable, alors qu’elle est la plus pratiquée en paléo-hydrologie. L’utilisation combinée du plomb-210, du césium-137, de l’analyse géochimique des sédiments et des informations sur les crues historiques a permis de discriminer et de dater les différents dépôts de crue de ce dernier siècle dans la cavité GG et la terrasse GE. / A multidating approach was carried out on slackwater flood deposits, preserved in valley side rock cave and terrace, of the Gardon River in Languedoc, southeast France. Lead-210, caesium-137, and geochemical analysis of mining-contaminated slackwater flood sediments have been used to reconstruct the history of these flood deposits. These age controls were combined with the continuous record of Gardon flow since 1890, and the combined records were then used to assign ages to slackwater deposits. The stratigraphic records of terrace GE and cave GG were excellent examples to illustrate the effects of erosion/preservation in a context of a progressively self-censoring, vertically accreting sequence. The sedimentary flood record of the terrace GE located at 10 m above the channel bed is complete for years post-1958 but incomplete before. During the 78-year period 1880-1958, 25 floods of a sufficient magnitude (> 1450 m3/s) have covered the terrace. Since 1958, however, the frequency of inundation of the deposits has been lower:only 5 or 6 floods in 52 years have been large enough to exceed the necessary threshold discharge (> 1700 m3/s). The progressive increase of threshold discharge and the reduced frequency of inundation at the terrace could allow stabilisation of the vegetation cover and improved protection against erosion from subsequent large magnitude flood events. The sedimentary flood record seems complete for cave GG located at 15 m above the channel bed. Here, the low frequency of events would have enabled a high degree of stabilisation of the sedimentary flood record, rendering the deposits less susceptible to erosion. Radiocarbon dating are used in this study and compared to the other dating techniques.Eighty percent of radiocarbon dates on charcoals were considerably older than those obtained by the other techniques in the terrace. On the other hand, radiocarbon dating on seeds provided better results. This discrepancy between radiocarbon dates on charcoal and seeds is explained by the nature of the dated material (permanent wood vs. annual production and resistance to degradation process). Finally, we showed in this study that although the most common dating technique used in paleoflood hydrology is radiocarbon dating, usually on charcoal preserved within slackwater flood sediments, this method did not permitus to define a coherent age model. Only the combined use of lead-210, caesium-137, and geochemical analysis of mining-contaminated sediments with the instrumental flood record can be applied to discriminate and date the recent slackwater deposits of the terrace GE and cave GG.
ISSN: 0169-555X

hal-01059669
https://hal.archives-ouvertes.fr/hal-01059669
https://hal.archives-ouvertes.fr/hal-01059669/document
https://hal.archives-ouvertes.fr/hal-01059669/file/ly2014-pub00040953.pdf
IRSTEA : PUB00040953

Éditeur(s) : HAL CCSD Elsevier
Résumé : International audience
In order to perform a human and ecological risk assessment of pharmaceutical products (PPs) in natural waters, it is necessary to accurately quantify a broad variety of PPs at low concentrations. Although numerous currently implemented analytical methodologies, less is known about the preservation of PPs in natural water samples within the period before analysis (holding time, storage conditions). This paper is the first literature review about the stability of PPs in natural waters (surface and groundwaters) during sample storage. The current work focuses on a comparison of the performances of the available preservation techniques (filtration, container materials, storage temperature, preservative agents, etc.) for PPs in samples. All 58 reviewed PPs may be successfully stabilized during 7 days in surface waters by at least one appropriate methodology regarding temperature, acidic and non-acidic preservatives. When temperature is not a sufficient preservation parameter f! or some PPs (hormones and fluoxetine) its combination with the addition of chemical agents into the samples may prolong the integrity of the PPs during storage in surface water. There is a strong need to use standard protocols to assess and compare the stability of PPs in environmental water matrices during storage as well as during analytical preparation or analysis (European criteria 2002/657/EC). Since the stability of PPs during sample storage is a critical parameter that could call into question the quality of the data provided for the concentrations, the design of stability studies should rigorously take into account all critical parameters that could impact the concentrations of the PPs with time.
Talanta

insu-00839186
https://hal-insu.archives-ouvertes.fr/insu-00839186
DOI : 10.1016/j.talanta.2013.01.042

Résumé : (System Details) Mode of access: World Wide Web.
(Dates or Sequential Designation) Began in 2009
Title from home page (viewed November 10, 2014).
This exhibition features selections from the Efraín Barradas Collection of Mexican & Cuban Film Posters collected and donated by Dr. Ramón A. Figueroa. The collection, which is preserved and housed in the Popular Culture Collections of the Department of Special and Area Studies Collections, consists of 378 film posters, lobby cards and window cards.
Cuba
Mexico

Droits : Permission granted to University of Florida to digitize and display this item for non-profit research and educational purposes. Any reuse of this item in excess of fair use or other copyright exemptions requires permission of the copyright holder.
895665469
ocn895665469
http://ufdc.ufl.edu/AA00027177/00001

Éditeur(s) : HAL CCSD Wiley-Blackwell
Résumé : International audience
The Lanzo peridotite massif is a fragment of oceanic lithosphere generated in an ocean-continent transition context and eclogitized during alpine collision. Despite the subduction history, the massif has preserved its sedimentary oceanic cover, suggesting that it may have preserved its oceanic structure. It is an exceptional case for studying the evolution of a fragment of the lithosphere from its oceanization to its subduction and then exhumation. We present a field and petrological study retracing the different serpentinization episodes and their impact on the massif structure. The Lanzo massif is composed of slightly serpentinized peridotites (<20% serpentinization) surrounded by an envelope of foliated serpentinites (100% serpentinization) bordered by oceanic metabasalts and metasedimentary rocks. The limit between peridotites and serpentinites defines the front of serpentinization. This limit is sharp: it is marked by the presence of massive serpentinites (80% serpentinization) and, locally, by dykes of metagabbros and mylonitic gabbros. The deformation of these gabbros is contemporaneous with the emplacement of the magma. The presence of early lizardite in the peridotites testifies that serpentinization began during the oceanization, which is confirmed by the presence of meta-ophicarbonates bordering the foliated serpentinite envelope. Two additional generations of serpentine occur in the ultramafic rocks. The first is a prograde antigorite that partially replaced the lizardite and the relict primary minerals of the peridotite during subduction, indicating that serpentinization is an active process at the ridge and in the subduction zone. Locally, this episode is followed by the deserpentinization of antigorite at peak P-T (estimated in eclogitized metagabbros at 2-2.5 GPa and 550-620 °C): it is marked by the crystallization of secondary olivine associated with chlorite and/or antigorite and of clinopyroxene, amphibole and chlorite assemblages. A second antigorite formed during exhumation partially to completely obliterating previous textures in the massive and foliated serpentinites. Serpentinites are an important component of the oceanic lithosphere generated in slow to ultraslow spreading settings, and in these settings, there is a serpentinization gradient with depth in the upper mantle. The seismic Moho limit could correspond to a serpentinization front affecting the mantle. This partially serpentinized zone constitutes a less competent level where, during subduction and exhumation, deformation and fluid circulation are localized. In this zone, the reaction kinetics are increased and the later steps of serpentinization obliterate the evidence of this progressive zone of serpentinization. In the Lanzo massif, this zone fully recrystallized into serpentinite during alpine subduction and collision. Thus, the serpentinite envelope represents the oceanic crust as defined by geophysicists, and the sharp front of serpentinization corresponds to an eclogitized seismic palaeo-Moho.
ISSN: 0263-4929

hal-00823051
https://hal.archives-ouvertes.fr/hal-00823051
DOI : 10.1111/jmg.12008

Éditeur(s) : George A. Smathers Libraries, University of Florida George A. Smathers Libraries, University of Florida ( Gainesville, FL )
Résumé : The UF Smathers Libraries partner with institutions and scholars to host and preserve digital collections, support scholarly publishing with digital collections, and support digital acquisitions to build shared collections. Clear benefits from partnerships are often supported with minimal costs by leveraging UF’s infrastructure for hosting, preservation, publishing, and acquisition. However, digital collections require ongoing support. In addition to the future “care and feeding,” new collections and projects also require support for various needs which may be one-time, infrequent, or a stable and continuous commitment throughout a project. These needs draw from digital curation and other areas (e.g., bulk ingesting materials, training for partners, liaising for grant proposal development, liaising for collection development and outreach, collaboration on activities related to specific projects as with training and outreach). Because of the immediate demands and ongoing commitments required for partnerships, potential new partnerships with the UF Libraries are reviewed for consideration and possible approval. This document provides background on partnerships with the UF Libraries, considerations for reviewing potential new partnerships for approval, and procedures for establishing new partnerships and ensuring optimal ongoing support.

Droits : [cc0] The author dedicated the work to the Commons by waiving all of his or her rights to the work worldwide under copyright law and all related or neighboring legal rights he or she had in the work, to the extent allowable by law.
http://ufdc.ufl.edu/AA00017119/00036

Éditeur(s) : HAL CCSD
Résumé : LPSC14037
Scientific data collected with modern sensors or dedicated detectors exceed very often the perimeter of the initial scientific design. These data are obtained more and more frequently with large material and human efforts. A large class of scientific experiments are in fact unique because of their large scale, with very small chances to be repeated and to superseded by new experiments in the same domain: for instance high energy physics and astrophysics experiments involve multi-annual developments and a simple duplication of efforts in order to reproduce old data is simply not affordable. Other scientific experiments are in fact unique by nature: earth science, medical sciences etc. since the collected data is "time-stamped" and thereby non-reproducible by new experiments or observations. In addition, scientific data collection increased dramatically in the recent years, participating to the so-called "data deluge" and inviting for common reflection in the context of "big data" investigations. The new knowledge obtained using these data should be preserved long term such that the access and the re-use are made possible and lead to an enhancement of the initial investment. Data observatories, based on open access policies and coupled with multi-disciplinary techniques for indexing and mining may lead to truly new paradigms in science. It is therefore of outmost importance to pursue a coherent and vigorous approach to preserve the scientific data at long term. The preservation remains nevertheless a challenge due to the complexity of the data structure, the fragility of the custom-made software environments as well as the lack of rigorous approaches in workflows and algorithms. To address this challenge, the PREDON project has been initiated in France in 2012 within the MASTODONS program: a Big Data scientific challenge, initiated and supported by the Interdisciplinary Mission of the National Centre for Scientific Research (CNRS). PREDON is a study group formed by researchers from different disciplines and institutes. Several meetings and workshops lead to a rich exchange in ideas, paradigms and methods. The present document includes contributions of the participants to the PREDON Study Group, as well as invited papers, related to the scientific case, methodology and technology. This document should be read as a "facts finding" resource pointing to a concrete and significant scientific interest for long term research data preservation, as well as to cutting edge methods and technologies to achieve this goal. A sustained, coherent and long term action in the area of scientific data preservation would be highly beneficial.
http://hal.in2p3.fr/in2p3-00959072

in2p3-00959072
http://hal.in2p3.fr/in2p3-00959072
http://hal.in2p3.fr/in2p3-00959072/document
http://hal.in2p3.fr/in2p3-00959072/file/PREDON_Scientific_Data_Preservation_2014.pdf

Éditeur(s) : HAL CCSD
Résumé : International audience
STUDY QUESTION: Do freezing and in vitro culture procedures enhance the expression of proteins involved in apoptotic or autophagic pathways in murine pre-pubertal testicular tissue? SUMMARY ANSWER: IVM strongly modified apoptosis- and autophagy-related relative protein levels in mice testicular tissue whereas the impact of cryopreservation procedures was minimal at the end of the culture. WHAT IS KNOWN ALREADY: In vitro spermatogenesis remains a challenging technical issue as it imposes to find a very close balance between survival and death of germ cell natural precursors (i.e. gonocytes and spermatogonia), which will eventually undergo a complete spermatogenesis close to in vivo conditions. The establishment of efficient culture conditions coupled with suitable cryopreservation procedures (e.g. controlled slow freezing [CSF] and solid surface vitrification [SSV]) of pre-pubertal testicular tissue is a crucial step in the fields of fertility preservation and restoration to improve the spermatic yield obtained in vitro. STUDY DESIGN, SIZE, DURATION: Here, we study cryopreservation procedures (i.e. CSF or SSV) and the impact of culture media compositions. A first set of 66 mouse pre-pubertal testes were directly cultured during 30, 36, 38 and 60 days (D) from 2.5 to 6.5-day-old CD-1 mice to evaluate the impact of time-aspect of culture and to endorse the reverse phase protein microarrays (RPPM) technique as an adapted experimental tool for the field of in vitro spermatogenesis. Ninety others fresh, slow-frozen and vitrified pre-pubertal testes were cultured during 30 days for the principal study to evaluate the impact of cryopreservation procedures before and after culture. Thirty-four testes dissected from 2.5, 6.5, 36.5, 40.5, 42.5 and 62.5 days postpartum (dpp) mice, corresponding to the time frames of spermatogenesis orchestrated in vitro, were used as in vivo controls. PARTICIPANTS/MATERIALS, SETTING, METHODS: After in vitro culture, testicular tissue samples originated from 2.5 or 6.5-day-old CD-1 male mice were analyzed using RPPM. This targeted proteomic technique allowed us to assess the expression level of 29 apoptosis- and autophagy-related factors by normalizing blank-corrected signal values. In addition, morphological analyses (e.g. HES, PAS, TRA98 and CREM) and DNA fragmentation in intra-tubular cells (i.e. terminal deoxynucleotidyl transferase dUTP nick end labeling; TUNEL) were assessed for the distinct experimental conditions tested as well as for in vivo control mouse testes. MAIN RESULTS AND THE ROLE OF CHANCE: A validation of the RPPM procedure in the field of in vitro spermatogenesis was completed with assay and array robustness before a principal study concerning the evaluation of the impact of in vitro culture and cryopreservation procedures. The proportion of elongated spermatids and the total cell number per seminiferous tubule tended to be very different between the in vivo and in vitro conditions (P < 0.05), suggesting the presence of a beneficial regulation on the first spermatogenesis wave by intrinsic apoptosis (Caspase₉) and autophagy (Atg5) factors (P < 0.0003 and r2 = 0.74). Concerning the impact of culture media compositions, a basic medium (BM) composed of αMEM plus 10% KnockOut™ serum replacement and gentamicin supplemented with retinol (Rol) and vitamin E (Vit. E) was selected as the best culture medium for fresh 6.5 dpp tissue cultured during 30D with 27.7 ± 8.10% of seminiferous tubules containing elongated spermatids. Concerning the impact of cryopreservation procedures, SSV did not have any impact on the morphological parameters evaluated after culture in comparison to fresh tissue (FT) controls. The proportion of tubules with elongated spermatids on testicular explants cultured with BMRol+Vit. E was not different between SSV (6.6 ± 1.6%) and CSF (5.3 ± 1.9%); however, round spermatids were observed more frequently for SSV (19 ± 6.2%) than CSF (3.3 ± 1.9%, P = 0.0317). Even if the proportion of TUNEL-positive cells for BMRol+Vit. E was higher at D30 after SSV (4.12 ± 0.26%) than CSF (1.86 ± 0.12%, P = 0.0022) and FT (2.69 ± 0.33%, P = 0.0108), the DNA damages observed at the end of the culture (i.e. D30) were similar to respective 6.5 dpp controls. In addition, the relative protein level expression ratio of an apoptotic factor, the phosphorylated FADD on Fas, was reduced by 64-fold in vitrified testes cultured with BMRol+Vit. E. Furthermore, we found in this study that the StemPro®-34 SFM culture medium supplemented with growth factors (e.g. EGF, bFGF, GDNF and LIF) prevented the differentiation of spermatogonial stem cells in favor of a significant proliferation with a better architectural pattern than in vivo 6.5 dpp controls with an increase of seminiferous tubules area for FT (P = 0.0357) and CSF (P = 0.0317). LIMITATIONS REASONS FOR CAUTION: Despite our promising results, the evaluation of apoptotic- and autophagic-related proteins was studied for a limited amount of proteins and on global testicular tissue. WIDER IMPLICATIONS OF THE FINDINGS: The data presented herein will help to improve apoptotic and autophagic understanding during the first spermatogenic wave. Moreover, our findings illustrate for the first time that, using finely-tuned experimental conditions, a testicular in vitro culture combined with proteomic technologies may significantly facilitate the study of cryopreservation procedures and in vitro culture evaluations. This study may also contribute to improve work on testicular tissues from pre-pubertal and adolescent cancer survivors. STUDY FUNDING/COMPETING INTEREST(S): This study was supported by a Ph.D. grant from the Rouen Normandie Université and a financial support from 'la Ligue nationale contre le cancer' (both awarded to L.D.), funding from Institute for Research and Innovation in Biomedicine (IRIB), Agence de la Biomédecine, and co-supported by European Union and Région Normandie. Europe gets involved in Normandie with European Regional Development Fund (ERDF). The authors declare that there is no conflict of interest.
Molecular Human Reproduction

hal-01647081
https://hal-univ-rennes1.archives-ouvertes.fr/hal-01647081
DOI : 10.1093/molehr/gax054
PUBMED : 29040674

Éditeur(s) : Université des Antilles Études caribéennes
Résumé : La présente étude a été menée dans les cantons de la préfecture de Doufelgou au Nord Togo. Sur la base de la beauté du paysage, des richesses naturelles et des enquêtes semi-structurées, le potentiel écotouristique de cette préfecture a été évalué. Le traitement des données spatiales collectées a permis de réaliser la carte écotouristique et de proposer un circuit touristique. Trois catégories de potentialités ont été répertoriées : les potentialités naturelles, les potentialités culturelles et les potentialités anthropiques. Ces potentialités peu valorisées subissent à des degrés variables diverses pressions susceptibles de les mettre en péril. Cet article présente ces diverses potentialités et analyse les opportunités socio-économique et environnementale d’un développement écotouristique dans cette zone.
This study was conducted in the cantons of Doufelgou prefecture in Northern Togo. On the basis of the beauty of the landscape, natural resources and semi-structured surveys, the potential ecotourism in this prefecture was evaluated. The treatment of spatial data collected helped make ecotourism map and to propose a tourist circuit. Three categories of potential have been identified: the natural potential, cultural potential and human potential. These potentialities are little valued and suffer to varying degrees various pressures which may put them at risk. This paper presents various potential and carries an analysis on socio-economic and environmental benefits of ecotourism development in this area.
Doufelgou
Togo

Droits : info:eu-repo/semantics/openAccess
urn:doi:10.4000/etudescaribeennes.6326
http://journals.openedition.org/etudescaribeennes/6326

Éditeur(s) : HAL CCSD
Résumé : 38 pages
Rapport
The Chicxulub impact crater, México, is unique. It is the only known terrestrial impact structure that has been directly linked to a mass extinction event and the only terrestrial impact with a global ejecta layer. Of the three largest impact structures on Earth, Chicxulub is the best preserved. Chicxulub is also the only known terrestrial impact structure with an intact, unequivocal topographic peak ring. Chicxulub’s role in the Cretaceous/Paleogene (K-Pg) mass extinction and its exceptional state of preservation make it an important natural laboratory for the study of both large impact crater formation on Earth and other planets and the effects of large impacts on the Earth’s environment and ecology. Our understanding of the impact process is far from complete, and despite more than 30 years of intense debate, we are still striving to answer the question as to why this impact was so catastrophic.During International Ocean Discovery Program (IODP) Expedition 364, Paleogene sediments and lithologies that make up the Chicxulub peak ring were cored to investigate (1) the nature and formational mechanism of peak rings, (2) how rocks are weakened during large impacts, (3) the nature and extent of post-impact hydrothermal circulation, (4) the deep biosphere and habitability of the peak ring, and (5) the recovery of life in a sterile zone. Other key targets included sampling the transition through a rare midlatitude section that might include Eocene and Paleocene hyperthermals and/or the Paleocene/Eocene Thermal Maximum (PETM); the composition and character of the impact breccias, melt rocks, and peak-ring rocks; the sedimentology and stratigraphy of the Paleocene–Eocene Chicxulub impact basin infill; the chronology of the peak-ring rocks; and any observations from the core that may help us constrain the volume of dust and climatically active gases released into the stratosphere by this impact. Petrophysical property measurements on the core and wireline logs acquired during Expedition 364 will be used to calibrate geophysical models, including seismic reflection and potential field data, and the integration of all the data will calibrate impact crater models for crater formation and environmental effects. The proposed drilling directly contributes to IODP Science Plan goals:Climate and Ocean Change: How resilient is the ocean to chemical perturbations? The Chicxulub impact represents an external forcing event that caused a 75% level mass extinction. The impact basin may also record key hyperthermals within the Paleogene.Biosphere Frontiers: What are the origin, composition, and global significance of subseafloor communities? What are the limits of life in the subseafloor? How sensitive are ecosystems and biodiversity to environmental change? Impact craters can create habitats for subsurface life, and Chicxulub may provide information on potential habitats for life, including extremophiles, on the early Earth and other planetary bodies. Paleontological and geochemical studies at ground zero will document how large impacts affect ecosystems and effects on biodiversity.Earth Connections/Earth in Motion: What are the composition, structure and dynamics of Earth’s upper mantle? What mechanisms control the occurrence of destructive earthquakes, landslides, and tsunami? Mantle uplift in response to impacts provides insight into dynamics that differ between Earth and other rocky planets. Impacts generate earthquakes, landslides, and tsunami, and scales that generally exceed plate tectonic processes yield insight into effects, the geologic record, and potential hazards.IODP Expedition 364 was a Mission Specific Platform expedition to obtain subseabed samples and downhole logging measurements from the sedimentary cover sequence and peak ring of the Chicxulub impact crater. A single borehole was drilled into the Chicxulub impact crater on the Yucatán continental shelf, recovering core from 505.7 to 1334.73 m below seafloor with ~99% core recovery and acquiring downhole logs for the entire depth.
International Ocean Discovery Program

hal-01639520
https://hal.archives-ouvertes.fr/hal-01639520
DOI : 10.14379/iodp.pr.364.2017