Éditeur(s) : Public Library Science
Résumé : Subsurface sediments of the Sonora Margin (Guaymas Basin), located in proximity of active cold seep sites were explored. The taxonomic and functional diversity of bacterial and archaeal communities were investigated from 1 to 10 meters below the seafloor. Microbial community structure and abundance and distribution of dominant populations were assessed using complementary molecular approaches (Ribosomal Intergenic Spacer Analysis, 16S rRNA libraries and quantitative PCR with an extensive primers set) and correlated to comprehensive geochemical data. Moreover the metabolic potentials and functional traits of the microbial community were also identified using the GeoChip functional gene microarray and metabolic rates. The active microbial community structure in the Sonora Margin sediments was related to deep subsurface ecosystems (Marine Benthic Groups B and D, Miscellaneous Crenarchaeotal Group, Chloroflexi and Candidate divisions) and remained relatively similar throughout the sediment section, despite defined biogeochemical gradients. However, relative abundances of bacterial and archaeal dominant lineages were significantly correlated with organic carbon quantity and origin. Consistently, metabolic pathways for the degradation and assimilation of this organic carbon as well as genetic potentials for the transformation of detrital organic matters, hydrocarbons and recalcitrant substrates were detected, suggesting that chemoorganotrophic microorganisms may dominate the microbial community of the Sonora Margin subsurface sediments.
Plos One (1932-6203) (Public Library Science), 2014-08 , Vol. 9 , N. 8 , P. e104427

Droits : This is an open-access article, free of all copyright, and may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose. The work is made available under the Creative Commons CC0 public domain dedication.
http://archimer.ifremer.fr/doc/00203/31408/29801.pdf
DOI:10.1371/journal.pone.0104427
http://archimer.ifremer.fr/doc/00203/31408/

Éditeur(s) : HAL CCSD Oxford University Press (OUP)
Résumé : International audience
We analyse P-wave receiver functions across the western Gulf of Aden and southern Red Sea continental margins in Western Yemen to constrain crustal thickness, internal crustal structure and the bulk seismic velocity characteristics in order to address the role of magmatism, faulting and mechanical crustal thinning during continental breakup. We analyse teleseismic data from 21 stations forming the temporary Young Conjugate Margins Laboratory (YOCMAL) network together with GFZ and Yemeni permanent stations. Analysis of computed receiver functions shows that (1) the thickness of unextended crust on the Yemen plateau is ∼35km; (2) this thins to ∼22km in coastal areas and reaches less than 14km on the Red Sea coast, where presence of a high-velocity lower crust is evident. The average Vp/Vs ratio for the western Yemen Plateau is 1.79, increasing to ∼1.92 near the Red Sea coast and decreasing to 1.68 for those stations located on or near the granitic rocks. Thinning of the crust, and by inference extension, occurs over a ∼130-km-wide transition zone from the Red Sea and Gulf of Aden coasts to the edges of the Yemen plateau. Thinning of continental crust is particularly localized in a <30-km-wide zone near the coastline, spatially co-incident with addition of magmatic underplate to the lower crust, above which on the surface we observe the presence of seaward dipping reflectors (SDRs) and thickened Oligo-Miocene syn-rift basaltic flows. Our results strongly suggest the presence of high-velocity mafic intrusions in the lower crust, which are likely either synrift magmatic intrusion into continental lower crust or alternatively depleted upper mantle underplated to the base of the crust during the eruption of the SDRs. Our results also point towards a regional breakup history in which the onset of rifting was synchronous along the western Gulf of Aden and southern Red Sea volcanic margins followed by a second phase of extension along the Red Sea margin.
ISSN: 0956-540X

hal-00825194
https://hal.archives-ouvertes.fr/hal-00825194
https://hal.archives-ouvertes.fr/hal-00825194/document
https://hal.archives-ouvertes.fr/hal-00825194/file/RF_Hakim2012_GJI.pdf
DOI : 10.1093/gji/ggt072

Éditeur(s) : HAL CCSD Elsevier
Résumé : We report a preliminary analysis on the relationships between drainage basin structure and genetic structure of populations of the European vector of fasciolosis, Galba truncatula. In the study area, 251 snails belonging to 12 populations were collected along different ditches of a same river network. Each snail was genotyped at six variable microsatellite loci. Our results show that all sample sites are characterized by a low level of polymorphism and a very high and significant heterozygote deficiency. Our data reveal a significant genetic differentiation, even at a small scale, and failed to delimit clear patterns of isolation by euclidian distance. Our study shows that genetic differentiation significantly increases with hydrographic distance along the streams (p < 0.002), in consistence with the hypothesis that dispersion along the stream is dependent on the direction of water flow. This study shows that relationships can exist between the organization of the hydrological network and population biology of a disease vector, which has strong potential applications to drainage network management issues.
ISSN: 1567-1348

hal-00496374
https://hal.archives-ouvertes.fr/hal-00496374
DOI : 10.1016/j.meegid.2010.01.005

Éditeur(s) : Elsevier
Résumé : High P-wave velocities (7.1-7.8 km/s) lower crustal bodies (LCBs) imaged along volcanic margins are commonly interpreted as plume and break-up-related thick mafic underplating. This interpretation is partly challenged in this paper based on new seismic observations and modelling of the outer Voring Basin (Norway). An exceptional strong amplitude reflection, the T Reflection, is particularly well defined below the North Gjallar Ridge (NGR) between 7 and 8 s TWT. The T Reflection is located near the volcanic lava flows emplaced during the NE Atlantic breakup (similar to 55-54 Ma ago) and coincides with the top of the LCB, forming a mid-crustal dome. Based on structural and temporal relationships, we show that the dome clearly influences the structural development of the NGR and predates the continental breakup at least by 10-15 Ma. Using a thermo-kinematical model, we tried also to investigate and quantify the relationships between the extension, LCB and the magmatic production. Modelling suggests that significant Paleocene-Early Eocene magmatism can be produced without any temperature anomaly in the mantle if differential stretching occurs during the break-up initiation. The conclusion of 2D thermo-kinematical parametric analysis is that the magmatic model predicts, either little extension (beta < 2) with no melting or high extension (beta > 5) with significant melting along the outer Voring Basin. We suggest that the continental part of the LCB could not necessarily be breakup-related and so magmatic, as has often been stated previously. It is concluded here that the continental part of the LCB observed beneath the outer Voring Basin may be partly (or fully) attributed to inherited, high-pressure granulite/eclogite lower crustal rocks. The real amount of mafic material emplaced along the outer Voring Basin could be 20-40% less than thought.
Tectonophysics (0040-1951) (Elsevier), 2006 , Vol. 412 , N. 3-4 , P. 255-278

Droits : 2005 Elsevier B.V. All rights reserved
http://archimer.ifremer.fr/doc/2006/publication-1042.pdf
DOI:10.1016/j.tecto.2005.10.038
http://archimer.ifremer.fr/doc/00000/1042/

Éditeur(s) : HAL CCSD Springer Verlag
Résumé : International audience
The purpose of this investigation was to characterize the management and prognosis of severe Pneumocystis jirovecii pneumonia (PJP) in human immunodeficiency virus (HIV)-negative patients. An observational cohort study of HIV-negative adults with PJP documented by bronchoalveolar lavage (BAL) through Gomori-Grocott staining or immunofluorescence, admitted to one intensive care unit (ICU) for acute respiratory failure, was undertaken. From 1990 to 2010, 70 patients (24 females, 46 males) were included, with a mean age of 58.6 ± 18.3 years. The mean Simplified Acute Physiology Score (SAPS)-II was 36.9 ± 20.4. Underlying conditions included hematologic malignancies (n = 21), vasculitis (n = 13), and solid tumors (n = 13). Most patients were receiving systemic corticosteroids (n = 63) and cytotoxic drugs (n = 51). Not a single patient received trimethoprim-sulfamethoxazole as PJP prophylaxis. Endotracheal intubation (ETI) was required in 42 patients (60.0 %), including 38 with acute respiratory distress syndrome (ARDS). In-ICU mortality was 52.9 % overall, reaching 80.9 % and 86.8 %, respectively, for patients who required ETI and for patients with ARDS. In the univariate analysis, in-ICU mortality was associated with SAPS-II (p = 0.0131), ARDS (p < 0.0001), shock (p < 0.0001), and herpes simplex virus (HSV) or cytomegalovirus (CMV) on BAL (p = 0.0031). In the multivariate analysis, only ARDS was associated with in-ICU mortality (odds ratio [OR] 23.4 [4.5-121.9], p < 0.0001). PJP in non-HIV patients remains a serious disease with high in-hospital mortality. Pulmonary co-infection with HSV or CMV may contribute to fatal outcome.
ISSN: 0934-9723

inserm-00872789
http://www.hal.inserm.fr/inserm-00872789
DOI : 10.1007/s10096-012-1730-7
PUBMED : 22930407

Éditeur(s) : HAL CCSD BioMed Central
Résumé : ICARE Study Group
International audience
BackgroundPopulation attributable risks (PARs) are useful tool to estimate the burden of risk factors in cancer incidence. Few studies estimated the PARs of oral cavity cancer to tobacco smoking alone, alcohol drinking alone and their joint consumption but none performed analysis stratified by subsite, gender or age. Among the suspected risk factors of oral cavity cancer, only PAR to a family history of head and neck cancer was reported in two studies. The purpose of this study was to estimate in France the PARs of oral cavity cancer to several recognized and suspected risk factors, overall and by subsite, gender and age.MethodsWe analysed data from 689 oral cavity cancer cases and 3481 controls included in a population-based case–control study, the ICARE study. Unconditional logistic regression models were used to estimate odds ratios (ORs), PARs and 95 % confidence intervals (95 % CI).ResultsThe PARs were 0.3 % (95 % CI −3.9 %; +3.9 %) for alcohol alone, 12.7 % (6.9 %–18.0 %) for tobacco alone and 69.9 % (64.4 %–74.7 %) for their joint consumption. PAR to combined alcohol and tobacco consumption was 74 % (66.5 %–79.9 %) in men and 45.4 % (32.7 %–55.6 %) in women. Among suspected risk factors, body mass index 2 years before the interview <25 kg.m−2, never tea drinking and family history of head and neck cancer explained 35.3 % (25.7 %–43.6 %), 30.3 % (14.4 %–43.3 %) and 5.8 % (0.6 %–10.8 %) of cancer burden, respectively. About 93 % (88.3 %–95.6 %) of oral cavity cancers were explained by all risk factors, 94.3 % (88.4 %–97.2 %) in men and only 74.1 % (47.0 %–87.3 %) in women.ConclusionOur study emphasizes the role of combined tobacco and alcohol consumption in the oral cavity cancer burden in France and gives an indication of the proportion of cases attributable to other risk factors. Most of oral cavity cancers are attributable to concurrent smoking and drinking and would be potentially preventable through smoking or drinking cessation. If the majority of cases are explained by recognized or suspected risk factors in men, a substantial number of cancers in women are probably due to still unexplored factors that remain to be clarified by future studies.
ISSN: 1471-2407

Droits : http://creativecommons.org/licenses/by/
hal-01258466
http://hal.upmc.fr/hal-01258466
http://hal.upmc.fr/hal-01258466/document
http://hal.upmc.fr/hal-01258466/file/BMCC.pdf
DOI : 10.1186/s12885-015-1841-5

Éditeur(s) : Université de Bretagne Occidentale
Résumé : The major objectives of this thesis were to study the structure, functioning and dynamics of different faunal assemblages associated with cold seep ecosystems in the eastern Mediterranean Sea. We focused our study on three main sites: the Marmara Sea, the Mediterranean ridge and the Nile deep sea fan. This thesis aims at determining the environmental signature of Mediterranean sites and the characteristics of the associated fauna at European and global scales. The specific objectives were to (1) determine the composition, diversity, density, biomass of invertebrate assemblages, (2) characterize biogeochemical conditions within different microhabitats in the vicinity of organisms, (3) to define the trophic relationships amongst seep fauna using stable isotope analyses (collaboration) and lastly, (4) to compare the spatial distribution and assemblage characteristics at different spatial scales (local, structure-scale, regional). The results show that (1) the Napoli mud volcano is the richest structure in terms of numbers of taxa while the Nile deep-sea fan sites need additional sampling, (2) a high variability is observed both between the different microhabitats but also within a single microhabitat, (3) the methane and oxygen gradients as well as substratum type are the main structuring factors explaining the distribution of the fauna and (4) the characteristics of the microhabitats, rather than the study area, influence the structure of benthic communities associated with Mediterranean cold seeps.
Les objectifs principaux de cette thèse sont d’étudier la structure, le fonctionnement et la dynamique de différents assemblages de faune associée aux émissions de fluides froids de Méditerranée orientale. Trois principaux sites ont été étudiés : la mer de Marmara, la ride méditerranéenne et le delta profond du Nil. Cette thèse tente de déterminer la « signature environnementale » des sites méditerranéens et les caractéristiques de la faune qui y est associée dans un contexte de marges européennes et mondiale. Les objectifs spécifiques sont (1) de déterminer la composition, la diversité, la densité et la biomasse des assemblages d'invertébrés ; (2) de caractériser les conditions biogéochimiques au sein des différents microhabitats à proximité des organismes; (3) de caractériser les relations trophiques sur les différents assemblages de faune par une approche isotopique (en collaboration) et finalement, (4) de comparer la distribution spatiale et les caractéristiques des assemblages à différentes échelles spatiales (locale, à l’échelle d’une structure géologique, régionale). Les résultats montrent que : (1) le volcan de boue Napoli est le site le plus riche en taxons alors que les sites du delta du Nil nécessiteraient un échantillonnage plus approfondi ; (2) il existe une grande variabilité entre les microhabitats ainsi qu’au sein d’un même microhabitat ; (3) les gradients en méthane, en oxygène ainsi que la nature du substrat sont les principaux facteurs abiotiques expliquant la distribution de la faune, (4) les caractéristiques des microhabitats, plutôt que la zone d’étude, influencent la structure des communautés benthiques associées aux écosystèmes de fluides froids méditerranéens.

Droits : 2010 The Author, UBO
http://archimer.ifremer.fr/doc/00020/13138/10178.pdf
http://archimer.ifremer.fr/doc/00020/13138/

Éditeur(s) : HAL CCSD Springer Verlag
Résumé : International audience
PURPOSE: Dairy farming is a risk factor for the development of chronic obstructive pulmonary disease (COPD). We assessed the prevalence of farming-induced COPD (FI-COPD) using a new screening device, and we analyzed symptoms and occupational risk factors. METHODS: We performed on-site screening study of bronchial obstruction using an electronic mini-spirometer (EMS) on an entire population of dairy farmers (n = 147) from two villages in Brittany, France. Suspected bronchial obstruction (FEV1/FEV6 <0.8) was confirmed with standardized lung function tests (FEV1/FVC ≤0.7). We assessed past medical histories, respiratory symptoms, and occupational tasks of subjects with bronchial obstruction; asthmatics were defined as atopic and/or reversible; smoking-induced COPD patients were defined as non-reversible, non-atopic with smoking histories (≥5 PY); and FI-COPD patients were defined as non-reversible, non-atopic, and non-smokers. RESULTS: Using the EMS, 30.6% (n = 45) of dairy farmers were suspected of bronchial obstruction and underwent standardized spirometry. The FEV1/FEV6 ratio and FEV1/FVC ratio were in good agreement (r² = 0.66, P < 0.0001). The prevalence of confirmed bronchial obstruction was 9.5% (n = 14), which included 4 asthmatics, 3 smoking-induced COPD subjects, and 7 FI-COPD subjects. All the COPD patients were GOLD Stage II, and none were aware of their respiratory disease. Foddering duration was significantly higher in FI-COPD subjects compared with non-obstructive subjects, with 44 versus 17 min/day, respectively. CONCLUSIONS: The EMS was a convenient mean of screening for bronchial obstruction, especially in on-site settings, and allowed us to diagnose FI-COPD in a non-spontaneously complaining dairy farmer population. Foddering was considered a significant risk factor.
ISSN: 0340-0131

hal-00872929
https://hal.archives-ouvertes.fr/hal-00872929
DOI : 10.1007/s00420-011-0708-6
PUBMED : 21986906

Résumé : Bacteriological study of abyssal sediments: During the Demeraby mission of the N/O Jean Charcot in the western Atlantic Ocean, a study of the microflora of the digestive tracts of echinoderms (Holothuroidea of various genera, Asteroidea, Echinoidea) was compared with that of the surrounding sediment. The bacterial concentration observed in the digestive tracts of all the animals studied is higher than that in the surrounding sediments. The largest numbers were always found in the most anterior part of the digestive tracts of the holothurians. In some cases, the increase is considerable (7.10 versus 5.2. 10 cells per millilitre): It indicates a proliferation of bacterial cells in the anterior part of the digestive tract. As the sediment moves through the digestive tract, the bacterial level progressively decreases. In the most posterior part, the concentration is, however, always clearly greater than that in the surrounding sediment. In the case of the holothurian of the genus Deima, analysis according to the Adamsonian principle of comparison shows that the microflora isolated from the various parts of the digestive tract is composed of taxonomic groups showing little resemblance to those isolated from the sediment. In the case of the holothurian of the genus Pseudostichopus, no specialisation of the microflora from digestive tract contents appears with respect to that of the ingested sediment. Examination of metabolic activities suggests a juxtaposition of two types of communities: Free sediment - sediment in transit that complement one other in their enzymatic and nutritional behaviour Indeed, the strains of free sediment seem to be rather suitable for hydrolysing and using carbohydrates, while those of enteric communities prefer the hydrolysis of lipids and proteins and the assimilation of fatty acids, dicarboxylic acids, hydroxylic acids and certain amino acids. Moreover, the enteric microflorae possess a greater nutritional versatility that, linked with its total numbers, has a sizeable catabolic potential. .../... -2- Measurements of heterotrophic activity done by means of 14 C glucose tracers make it possible to show that the metabolic activities of the bacterial communities of superficial abyssal sediments vary according to zones. In the Amazon Cone, a clear glucose mineralization gradient over a distance of a few kilometres can be observed. In the abyssal plain of Demerara heterotrophic activities are slowed in relation to those of the superficial sediments of the Cone of the Amazon, even though the bacterial numbers and the depths are of the same order of magnitude in the two zones. The abundant microflorae associated with the various divisions of digestive tract contents of the Deima abyssal holothurian manifest little metabolic activity as regards the glucose. However, the bacterial communities of Pseudostichopus and Psychropotes manifest very strong heterotrophic activities, increasing from the oesophagus towards the cloaca. Current results are too fragmentary to allow us to observe possible physiological specialisation of enteric bacterial communities enabling the facilitation of digestion processes in the host. Conclusion: In the abyssal environment, characterised by poorly developed bacterial communities, microflorae associated with the digestive tract contents of echinoderms have considerably greater cellular concentrations. By means of their enzymatic equipment, these enteric communities, which complement those of the free microflorae of superficial sediments, can participate in the transformation of organic matter in the abyssal area. Study of the dynamics of microbial communities and their heterotrophic activities in shellfish farms: From experiments done on the water in the Arcachon basin, trials to quantify and describe the reactivity potential of bacterial communities were performed. Bacterial communities were defined by direct counts, counts after setting them up for cultivation, structure and diversity of these communities, dosage of AMP, ADP, ATP. Two opposite ecological conditions were considered: Autumn conditions (high temperature) and a winter conditions (low temperature). Tubs of sea water of 6 and 60 litres were enriched either with ammonium chloride or a mixture of amino acids, at about 500 uAtg N.L. The experiments lasted 24 hours, with sampling every 3 hours. The autumn community reacted rapidly (6 hours), the response to the enrichment in organic nitrogen being the highest. The structure of the community has unstable immature stages. On the contrary, during the winter experiment, no response could be perceived. This may mean that the latency phase is greater than 24 hours. The structure of this community has mature stages. In conclusion, bacterial communities react in the same way as macroscopic communities. Stable communities are diversified and correspond to mature stages of the ecosystem; the immature stages are unstable and less diversified. This type of experiment has made it possible to bring to light the rapidity of response of bacterial populations, and can be used to define the stability of a bacterial community in a precise biotope.
ETUDE BACTERIOLOGIQUE DES SEDIMENTS ABYSSAUX RESUME Au cours de la mission DEMERABY du n/o Jean CHARCOT en atlantique occidental , une étude de la microflore des tractus digestifs d'Echinodermes (Holothurides de différents genres, Astérides, Echinides) a été comparée à celle du sédiment environnant. La concentration bactérienne observée dans les tractus digestifs de tous les animaux étudiés est supérieure à celle des sédiments environnants. Les effectifs les plus importants sont toujours observés dans la partie la plus antérieure des tractus digestifs des holothuries. Dans certains cas, l'accroissement est considérable (7.10 contre 5,2. 10 cellules/ml) : il indique une prolifération des cellules bactériennes dans la partie antérieure du tractus digestif. Au.cours de la progression du sédiment dans le tractus digestif, la charge bactérienne décroit progressivement. Dans la partie la plus postérieure, la concentration est cependant toujours nettement supérieure à celle du sédiment environnant-. Dans le cas d'une holothurie du genre Deima, l'analyse selon le principe adansonien de comparaison montre que la microflore isolée des différentes parties du tractus digestif est constituée de groupes taxonomiques présentant peu de ressemblance avec ceux isolés du sédiment. Dans le cas d'une holothurie du genre Pseudostichopus il n'apparait pas de spécialisation de la microflore du contenu du tractus digestif par rapport à celle du sédiment ingéré. ¿ ¿ . L'étude des activités métaboliques suggère une juxtaposition de deux types de communautés : sédiment libre-sédiment en transit qui se complètent dans leur comportement enzymatique et nutritionnel. En effet les souches du sédiment libre semblent être plutôt aptes à hydrolyser et à utiliser les glucides, tandis que celles des communautés entériques portent leur préférence sur l'hydrolyse des lipides et protéines et 1'assimilation des acides gras, acides dicarboxyl iques, hydro'xy-acides et certains acides aminés. D'autre part les microflores entériques possèdent une versatilité nutritionnelle plus importante qui, liée à leurs effectifs, offre une potentialité catabolique non négligeable. .../... -2- Les mesures d'activité hétérotrophe effectuées au moyen de traceurs glucose 14 C permettent de montrer que les activités métaboliques des communautés bactériennes des sédiments abyssaux superficiels varient selon les zones. Dans le Cone de l'Amazone on observe un net gradient de minéralisation du glucose sur une distance de quelques kilomètres. Dans la plaine abyssale de Demerara les activités hétérotrophessont ralenties par rapport à celles des sédiments superficiels du Cone de l'Amazone, bien que les effectifs bactériens et les profondeurs soient du même ordre de grandeur dans ces deux zones. Les abondantes microflores associées aux différentes fractions du contenu du tractus digestif de l'holothurie abyssale Deima ne manifestent que peu d'activités métaboliques vis à vis du glucose. Par contre les communautés . bactériennes de Pseudostichopus et P syahropotes manifestent des activités hétérotrophes très fortes, croissant de 1'oesophage vers le cloaque. Les résultats actuels sont trop fragmentaires pour permettre d'observer une éventuelle spécialisation physiologique des communautés bactériennes entëriques permettant de faciliter les processus de digestion chez l'hôte. Conclusion: dans le milieu abyssal, caractérisé par des communautés bactériennes faiblement développées, les microflores associées aux contenus de tractus digestif des Echinodermes présentent des concentrations cellulaires considérablement plus importantes. Par leurs équipements enzymâtiques ces communautés entëriques complémentaires de ceux des microflores libres des sédiments superficiels, peuvent participer à la transformation des matières organiques du domaine abyssal. ETUDE DE LA DYNAMIQUE DES COMMUNAUTES MICROBIENNES ET DE LEURS ACTIVITES HETEROTROPHES DANS LES PARCS CONCHYLICOLES RESUME A partir d'expérimentations effectuées sur l'eau du bassin d'Arcachon, des essais de quantification et qualification du potentiel de réactivité des communautés bactériennes ont été effectuées. Les communautés bactériennes ont été définies par comptage directs, comptages après mise en culture, structure et diversité de ces communautés, dosage des AMP, ADP, ATP. Deux situations écologiques opposées ont étées considérées . Une situation automnale (température élevée) et une situation hivernale (température faible). Des bacs d'eau de mer de 6 et 60 litres ont été enrichis , soit avec du chlorure d'ammonium, soit avec un mélange d'acides aminés, à environ 500 uAtg N.l" . Les expériences ont duré 24 heures, avec des prélèvements toutes les 3 heures. La communauté automnale réagit rapidement (6 heures), la ' réponse à l'enrichissement en azote organique étant la plus importante. La structure de la communauté présente des stades immatures instables. Au contraire, au cours de l'expérimentation hivernale, aucune réponse n'a pu être perçue. Ceci peut signifier que la phase de latence est supérieure à 24 heures. La structure . de cette communauté présente des stades matures. En Conclusion, les communautés bactériennes réagissent de la même façon que les communautés macroscopiques. Les communautés stables sont diversifiées et correspondent à des stades matures de l'écosystème, les stades immatures sont instables et moins diversifiés. Ce type d'expérience a permi de mettre en évidence la rapidité de réponse des populations bactériennes, et peut être utilisé pour définir la stabilité d'une communauté bactérienne dans un biotope précis.

Droits : info:eu-repo/semantics/openAccess
http://archimer.ifremer.fr/doc/1981/rapport-1926.pdf
http://archimer.ifremer.fr/doc/00000/1926/

Éditeur(s) : HAL CCSD Oxford University Press (OUP): Policy P - Oxford Open Option A Oxford University Press (OUP)
Résumé : International audience
It is well known that sedimentary rocks having same porosity can have very different pore size distribution. The pore distribution determines many characteristics of the rock, among which its transport properties are often the most useful. Multifractal analysis is a powerful tool that is increasingly used to characterize the pore space. In this study, we performed multifractal analysis of pore distribution on simulated sedimentary rocks using the relaxed bidisperse ballistic deposition model (RBBDM). The RBBDM can generate a 3-D structure of sedimentary rocks of variable porosity by tuning the fraction p of particles of two different sizes. We also performed multifractal analysis on two samples of real sedimentary rock to compare with the simulation studies. One sample, an oolitic limestone is of high porosity (40 per cent) while the other is a reefal carbonate of low porosity, around 7 per cent. 2-D sections of X-ray microtomographs of the real rocks were stacked sequentially to reconstruct the real rock specimens. Both samples show multifractal character. The results from analysis of real rock agree quite well with the simulated structure of low porosity. The simulated rock of high porosity showed a weak multifractal nature though the real rock sample of similar porosity was found to be strongly multifractal. We propose a ‘structure parameter’ ξ which is a function of porosity and the generalized dimensions, and controls the transport properties of the rock.
ISSN: 0956-540X

hal-01174142
https://hal.archives-ouvertes.fr/hal-01174142
DOI : 10.1093/gji/ggu417

Résumé : Ifremer launched and coordinated the development of a strategy for the REBENT network (REseau BENThique) in 2000 to monitor the aftermath of the "Erika" oil spill in December 1999. Its aim is to provide consistent baseline knowledge about coastal benthic habitats and constitute a monitoring tool to detect changes at various scales over time and space. Sector-based seabed habitat mapping in shallow water (< 50 m deep), is currently being conducted throughout Brittany's coastal waters through a combination of geoacoustic marine systems and ground-truthing using biological grab sampling and seabed observations. Sedimentological and biological results on Glenan area emphasize a great diversity of subtidal habitats and marine species recognized. Sidescan imagery accentuates the complexity of the communities structure in a marine environment distinguished by strong and regulary roughness of the sea. Maerl biocenose is present at the nord-east of the archipelago. Progressively it gives way to mud sediment colonized by Amphiura filiformis and Haploops in the deeper channel. To the west and the south, substratum types are more coarse, occasionally mobiles, composed of essentially sand community with Nephtys and gravelly sand community with Branchiostoma lanceolatum.
Le Rebent (Réseau benthique), initié en 2000 à la suite de la catastrophe de l'Erika, a pour objectif de suivre les habitats benthiques côtiers et de détecter les changements à différentes échelles de temps et d'espace. L'approche sectorielle dans les petits fonds (< 50 m) des côtes bretonnes combine, des moyens de prospection acoustique, afin de délimiter les principaux types de substrats, avec des prélèvements et des observations biologiques pour caractériser les peuplements macrobenthiques. Les résultats des analyses sédimentologiques et faunistiques montrent autour de l'archipel des Glénan, une grande diversité d'habitats et d'espèces reconnues. L'imagerie acoustique fait ressortir la complexité de leur structuration dans un environnement marin soumis à de fortes et régulières périodes d'agitation. La biocénose de maërl occupe le nord-est de l'archipel. Elle laisse place progressivement aux sédiments envasés à Amphiura filiformis et aux vases à Haploops dans le fond du chenal. A l'ouest et au sud, les subtrats sont plus grossiers, essentiellement sableux à Nephtys et sablo-graveleux à Branchiostoma lanceolatum, épisodiquement mobiles.

Droits : info:eu-repo/semantics/openAccess
http://archimer.ifremer.fr/doc/2006/rapport-2301.pdf
http://archimer.ifremer.fr/doc/2006/sup-2301.pdf
http://archimer.ifremer.fr/doc/00000/2301/

Éditeur(s) : HAL CCSD Springer Verlag
Résumé : International audience
Bronchoalveolar lavage (BAL) is a major diagnostic tool in lung diseases, including viral respiratory infections. We aimed to better define the situations where viral tests should be performed on BAL fluid (BALF). We retrospectively studied all cases where viral tests [immunofluorescence, immunocytochemistry, viral culture, and/or polymerase chain reaction (PCR)] were performed on BALF during a period of 1 year (2008) in our institution. We compared the characteristics of patients with virus-positive versus virus-negative BALF. Of the 636 BALF samples sent to the microbiology laboratory, 232 underwent viral tests. Of these, 70 (30 %) were positive and identified 85 viruses: herpes simplex virus (HSV)-1 (n = 27), cytomegalovirus (CMV, n = 23), Epstein-Barr virus (EBV, n = 18), human herpesvirus (HHV)-6 (n = 12), respiratory syncytial virus (RSV, n = 3), rhinovirus (n = 1), and adenovirus (n = 1). The variables associated with positive viral tests on univariate analysis were immunosuppression [human immunodeficiency virus (HIV), corticosteroids >10 mg/day for ≥3 weeks, or other immunosuppressive therapy], ground-glass attenuations on computed tomography (CT) scanning, late-onset ventilator-associated pneumonia (VAP), and durations of (i) hospital stay, (ii) intensive care unit (ICU) stay, and (iii) mechanical ventilation before BAL (p < 0.01 for each comparison). On multivariate analysis, only immunosuppression [odds ratio (OR) 6.4, 95 % confidence interval (CI) [2.8-14.3], p < 0.0001] and ground-glass attenuations (OR 3.7, 95 % CI [1.8-7.7], p = 0.0004) remained associated with virus-positive BAL. None of the viral tests performed on BALF for the initial assessment of diffuse infiltrative lung disease (n = 15) was positive. PCR improved the diagnostic yield of viral tests on BALF by 50 %. Testing for viruses on BALF should be mostly restricted to immunocompromised patients with acute respiratory diseases and/or patients with unexplained ground-glass attenuations on CT scanning.
ISSN: 0934-9723

hal-00873158
https://hal.archives-ouvertes.fr/hal-00873158
DOI : 10.1007/s10096-012-1791-7
PUBMED : 23238685

Éditeur(s) : Elsevier
Résumé : We investigated the influence of bioturbation by macrofauna on the vertical distribution of living (stained) benthic foraminifera in marine intertidal sediments. We investigated the links between macrofaunal bioturbation and foraminiferal distribution, by sampling from stations situated on a gradient of perturbation by oyster-farming, which has a major effect on benthic faunal assemblages. Sediment cores were collected on the French Atlantic coast, from three intertidal stations: an oyster farm, an area without oysters but affected by oyster biodeposits, and a control station. Axial tomodensitometry (CT-scan) was used for three-dimensional visualization and two-dimensional analysis of the cores. Biogenic structure volumes were quantified and compared between cores. We collected the macrofauna, living foraminifera, shells and gravel from the cores after scanning, to validate image analysis. We did not investigate differences in the biogenic structure volume between cores. However, biogenic structure volume is not necessarily proportional to the extent of bioturbation in a core, given that many biodiffusive activities cannot be detected on CT-scans. Biodiffusors and larger gallery-diffusors were abundant in macrofaunal assemblage at the control station. By contrast, macrofaunal assemblages consisted principally of downward-conveyors at the two stations affected by oyster farming. At the control station, the vertical distribution of biogenic structures mainly built by the biodiffusor Scorbicularia plana and the large gallery-diffusor Hediste diversicolor was significantly correlated with the vertical profiles of living foraminifera in the sediment, whereas vertical distributions of foraminifera and downward-conveyors were not correlated at the station affected by oyster farming. This relationship was probably responsible for the collection of foraminifera in deep sediment layers (> 6 cm below the sediment surface) at the control station. As previously suggested for other species, oxygen diffusion may occur via the burrows built by S. plana and H. diversicolor, potentially increasing oxygen penetration and providing a favorable microhabitat for foraminifera in terms of oxygen levels. By contrast, the absence of living foraminifera below 6 cm at the stations affected by oyster farming was probably associated with a lack of biodiffusor and large gallery-diffusor bioturbation. Our findings suggest that the effect of macrofaunal bioturbation on the vertical distribution of foraminiferal assemblages in sediments depends on the effects of the macrofauna on bioirrigation and sediment oxidation, as deduced by Eh values, rather than on the biogenic structure volume produced by macrofauna. The loss of bioturbator functional diversity due to oyster farming may thus indirectly affect infaunal communities by suppressing favorable microhabitats produced by bioturbation.
Journal of Experimental Marine Biology and Ecology (0022-0981) (Elsevier), 2009-03 , Vol. 371 , N. 1 , P. 20-33

Droits : 2009 Elsevier B.V. All rights reserved.
http://archimer.ifremer.fr/doc/2009/publication-6146.pdf
DOI:10.1016/j.jembe.2008.12.012
http://archimer.ifremer.fr/doc/00000/6146/

Éditeur(s) : HAL CCSD
Résumé : International audience
STUDY QUESTION: Do freezing and in vitro culture procedures enhance the expression of proteins involved in apoptotic or autophagic pathways in murine pre-pubertal testicular tissue? SUMMARY ANSWER: IVM strongly modified apoptosis- and autophagy-related relative protein levels in mice testicular tissue whereas the impact of cryopreservation procedures was minimal at the end of the culture. WHAT IS KNOWN ALREADY: In vitro spermatogenesis remains a challenging technical issue as it imposes to find a very close balance between survival and death of germ cell natural precursors (i.e. gonocytes and spermatogonia), which will eventually undergo a complete spermatogenesis close to in vivo conditions. The establishment of efficient culture conditions coupled with suitable cryopreservation procedures (e.g. controlled slow freezing [CSF] and solid surface vitrification [SSV]) of pre-pubertal testicular tissue is a crucial step in the fields of fertility preservation and restoration to improve the spermatic yield obtained in vitro. STUDY DESIGN, SIZE, DURATION: Here, we study cryopreservation procedures (i.e. CSF or SSV) and the impact of culture media compositions. A first set of 66 mouse pre-pubertal testes were directly cultured during 30, 36, 38 and 60 days (D) from 2.5 to 6.5-day-old CD-1 mice to evaluate the impact of time-aspect of culture and to endorse the reverse phase protein microarrays (RPPM) technique as an adapted experimental tool for the field of in vitro spermatogenesis. Ninety others fresh, slow-frozen and vitrified pre-pubertal testes were cultured during 30 days for the principal study to evaluate the impact of cryopreservation procedures before and after culture. Thirty-four testes dissected from 2.5, 6.5, 36.5, 40.5, 42.5 and 62.5 days postpartum (dpp) mice, corresponding to the time frames of spermatogenesis orchestrated in vitro, were used as in vivo controls. PARTICIPANTS/MATERIALS, SETTING, METHODS: After in vitro culture, testicular tissue samples originated from 2.5 or 6.5-day-old CD-1 male mice were analyzed using RPPM. This targeted proteomic technique allowed us to assess the expression level of 29 apoptosis- and autophagy-related factors by normalizing blank-corrected signal values. In addition, morphological analyses (e.g. HES, PAS, TRA98 and CREM) and DNA fragmentation in intra-tubular cells (i.e. terminal deoxynucleotidyl transferase dUTP nick end labeling; TUNEL) were assessed for the distinct experimental conditions tested as well as for in vivo control mouse testes. MAIN RESULTS AND THE ROLE OF CHANCE: A validation of the RPPM procedure in the field of in vitro spermatogenesis was completed with assay and array robustness before a principal study concerning the evaluation of the impact of in vitro culture and cryopreservation procedures. The proportion of elongated spermatids and the total cell number per seminiferous tubule tended to be very different between the in vivo and in vitro conditions (P < 0.05), suggesting the presence of a beneficial regulation on the first spermatogenesis wave by intrinsic apoptosis (Caspase₉) and autophagy (Atg5) factors (P < 0.0003 and r2 = 0.74). Concerning the impact of culture media compositions, a basic medium (BM) composed of αMEM plus 10% KnockOut™ serum replacement and gentamicin supplemented with retinol (Rol) and vitamin E (Vit. E) was selected as the best culture medium for fresh 6.5 dpp tissue cultured during 30D with 27.7 ± 8.10% of seminiferous tubules containing elongated spermatids. Concerning the impact of cryopreservation procedures, SSV did not have any impact on the morphological parameters evaluated after culture in comparison to fresh tissue (FT) controls. The proportion of tubules with elongated spermatids on testicular explants cultured with BMRol+Vit. E was not different between SSV (6.6 ± 1.6%) and CSF (5.3 ± 1.9%); however, round spermatids were observed more frequently for SSV (19 ± 6.2%) than CSF (3.3 ± 1.9%, P = 0.0317). Even if the proportion of TUNEL-positive cells for BMRol+Vit. E was higher at D30 after SSV (4.12 ± 0.26%) than CSF (1.86 ± 0.12%, P = 0.0022) and FT (2.69 ± 0.33%, P = 0.0108), the DNA damages observed at the end of the culture (i.e. D30) were similar to respective 6.5 dpp controls. In addition, the relative protein level expression ratio of an apoptotic factor, the phosphorylated FADD on Fas, was reduced by 64-fold in vitrified testes cultured with BMRol+Vit. E. Furthermore, we found in this study that the StemPro®-34 SFM culture medium supplemented with growth factors (e.g. EGF, bFGF, GDNF and LIF) prevented the differentiation of spermatogonial stem cells in favor of a significant proliferation with a better architectural pattern than in vivo 6.5 dpp controls with an increase of seminiferous tubules area for FT (P = 0.0357) and CSF (P = 0.0317). LIMITATIONS REASONS FOR CAUTION: Despite our promising results, the evaluation of apoptotic- and autophagic-related proteins was studied for a limited amount of proteins and on global testicular tissue. WIDER IMPLICATIONS OF THE FINDINGS: The data presented herein will help to improve apoptotic and autophagic understanding during the first spermatogenic wave. Moreover, our findings illustrate for the first time that, using finely-tuned experimental conditions, a testicular in vitro culture combined with proteomic technologies may significantly facilitate the study of cryopreservation procedures and in vitro culture evaluations. This study may also contribute to improve work on testicular tissues from pre-pubertal and adolescent cancer survivors. STUDY FUNDING/COMPETING INTEREST(S): This study was supported by a Ph.D. grant from the Rouen Normandie Université and a financial support from 'la Ligue nationale contre le cancer' (both awarded to L.D.), funding from Institute for Research and Innovation in Biomedicine (IRIB), Agence de la Biomédecine, and co-supported by European Union and Région Normandie. Europe gets involved in Normandie with European Regional Development Fund (ERDF). The authors declare that there is no conflict of interest.
Molecular Human Reproduction

hal-01647081
https://hal-univ-rennes1.archives-ouvertes.fr/hal-01647081
DOI : 10.1093/molehr/gax054
PUBMED : 29040674

Résumé : 1 - Study's goals review The management of Norway Lobster and Hake fisheries can be considered as the most important issue in the Gascogne gulf. Calculation methods used to evaluate prediction variations under various efforts and mesh constraints all pinpoint to the crucial role narrow mesh fishing plays in Hakes' Northern stock status. The involvement of Gascogne gulf Norway Lobster fisheries regarding Hakes over exploitation seems over dimensioned when comparing the size of both of these stocks. Hake stocks spread from Northern Great Britain to Northern Spain, while Norway Lobster stocks are located in the Gascogne gulf's mud flats. The biological solution is well known and consists in globally increasing mesh nets to 80mm which would bring up Hakes yield to its most. It would be unreal to pretend all parties involved in Hake stocks will come to use them. For now, it is mostly an economical issue since many vessels continue on with an exploitation scheme dating from the 60's and fish on coastal mud flats which are the most exploited zones and where their catch is detrimental to juvenile class Hakes and Norway Lobsters. Considering our limited time, this study will cover bio- economical modelisation preparation. You will therefore find a description of fisheries and an exploitation assessment based on 1986's available data. 2 - Accomplished work 2. 1 Fishery description Presenting the problems and preparing ulterior analyses was first and foremost. Norway Lobster vessels supposedly, according to evaluation models used, test immature Hakes in such a manner that all stock exploitation is jeopardized. Norway Lobster distribution is closely linked to the nature of the ocean floor since it can only build its habitat on fine sediment. Hakes' habitat is not as restrictive and is linked to food supply, especially euphausiid shrimps, so they can be found on sandy and sandy-sedimental floors. Norway Lobsters are sought by a fleet of 400 to 450 ships which characteristics widely vary according to their home port. On the northern part, fisheries are close to exploitation ports where Norway Lobsters and miscellaneous catch yield is sufficient to support an older fleet. On the southern part, the structure is diversified and flexibility usually rules, more modern vessels can easily switch their target on a seasonal or daily basis. Globally, Hakes' northern stocks are slightly on the rise. This rise is probably due to global mesh size increase, even if slight and a decrease on fishing effort where immature species are caught. Norway Lobster stocks unloading is stable or slightly lower and yields, as far as northern fishery are concerned, vary and oscillate between ±15 to ±20 % at about an average of 100 kg/day. Biological parameters used in the evaluations for each species are those used in CIEM and CEE work cells. Among those parameters, those describing growth are the greatest. For Hakes, an intermediary growth equation between males and females has been used. For Norway Lobsters, both sexes are treated separately. Simulations were carried out according to 3 types of data: Norway Lobster vessels fishing effort variations. Norway Lobster vessels mesh increase. Norway Lobster vessels fishing effort decrease or mesh increasing go along in the same direction for Hake and Norway Lobster stock production (chart A and B). Lastly, a brief study on results evaluation significance was done to modify: - the size of Hake rejects by Norway Lobster vessels. The range of sizes stays the same but the amount in each class varies according to the same percentage. - Norway Lobsters growth parameters, both values used (L» = 76 mm and k = 0.11 for males, L°° = 56 mm and k = 0.14 for females) representing the "high" hypothesis. In the first case, a 20% error rate for rejects evaluation with 60 mm mesh would only bring a 3% modification rate on global stock production. Those first results are contradicting usual evaluation conclusions and it will be necessary to recalculate by modifying the size class step. In the second case, to decrease L» and increase growth speed, in time production variations remain within usual inter annual fluctuations. In all figure cases, calculations confirming greater yield for in time production through hypothetical mesh size increase to 60 mm, are definitely well founded.
1 - Rappel des buts de l'étude La gestion des pêcheries de merlu et de langoustine peut être considérée comme le problème le plus important du Golfe de Gascogne. Les méthodes de calcul employées pour évaluer les variations de la production sous diverses contraintes d'effort et de maillage mettent toutes l'accent sur le rôle capital des pêcheries à petits maillages sur l'état du stock nord de merlu. Le niveau de responsabilité des pêcheries de langoustine du Golfe de Gascogne dans la surexploitation du merlu semble démesuré eu égard aux dimensions respectives des deux stocks. Le stock de merlu s'étend du Nord des Iles Britanniques au Nord de l'Espagne alors que le stock de langoustine est localisé aux vasièrés du Golfe de Gascogne. La solution biologique du problème est connue et tient à une augmentation généralisée des maillages à 80 mm qui amènerait le rendement du stock de merlu à son maximum. Il serait illusoire de prétendre que tous les acteurs opérant sur le stock de merlu arriveront jamais à l'utiliser. Pour le moment, le problème se pose essentiellement en termes économiques car beaucoup de navires poursuivent un schéma d'exploitation hérité des années 1960 et pèchent sur des vasièrés côtières qui sont les zones les plus exploitées et où ils effectuent leurs captures au détriment des jeunes classes d'âge de merlu et de langoustine. En raison des délais impartis, cette étude constitue la préparation à une modélisation bioéconomique. On y trouvera donc une description des pêcheries et un bilan de l'exploitation sur les données disponibles en 1986. 2 - Travaux effectués 2 . 1 Description de la pêcherie Il s'agissait avant tout de poser les problèmes et de préparer les analyses ultérieures. Les langoustiniers sont présumés, d'après les modèles d'évaluation employés, effectuer sur les immatures de merlu des prélèvements tels qu'ils mettent en danger toute l'exploitation du stock. La distribution de la langoustine est très liée à la nature du fond car elle ne peut construire son terrier que sur des fonds de vase fine. Celle du merlu est beaucoup plus large et inféodée à la recherche de nourriture, crevettes euphausiacées en particulier, on le trouve donc sur les fonds sableux à sablo-vaseux. La langoustine est recherchée par une flottille de 400 à 450 bateaux dont les caractéristiques sont très variables suivant leur appartenance géographique. Au nord, la pêcherie est proche des ports exploitants et les rendements en langoustine et en prise accessoire sont suffisants pour faire vivre une flottille ancienne. Vers le sud, la structure est très diversifiée et la flexibilité est généralement la règle et les navires de construction récente peuvent changer de cible aisément de façon saisonnière ou au cours de la même journée de pêche. Globalement, les débarquements de merlu du stock nord sont en légère hausse. Ce redressement est dû probablement aux augmentations de maillage, même légères, généralisées et à une baisse de l'effort sur les pêcheries où s'effectuent des captures d'immatures. Les débarquements de langoustine sont stables ou en légère baisse et les rendements, au moins sur la pêcherie du nord, varient avec des oscillations de ±15 à ±20 % autour d'une moyenne de 100 kg/jour. Les paramètres biologiques utilisés pour chaque espèce dans les évaluations sont ceux en usage dans les groupes de travail du CIEM et de la CEE. Parmi ces paramètres, ceux décrivant la croissance sont les plus importants. Pour le merlu une équation de croissance intermédiaire entre mâles et femelles a été utilisée. Pour la langoustine les deux sexes sont traitées séparément. Les simulations ont porté sur trois types de données : Variations de l'effort des langoustiniers Augmentation du maillage des langoustiniers La diminution de l'effort des langoustiniers ou L'augmentation de maillage agisse très exactement dans le même sens sur la production des stocks de merlu et de langoustine. Enfin une étude succincte de la sensibilité des résultats des évaluations a été effectuée pour des modifications : - de la composition en taille des rejets de merlu par les langoustiniers. L'étendue des tailles reste la même mais le nombre dans chaque classe varie selon le même pourcentage. des paramètres de croissance de la langoustine, le couple de valeurs utilisées (L» = 76 mm et k = 0.11 pour les mâles, L°° = 56 mm et k = 0.14 pour les femelles) constituant l'hypothèse "haute" Dans le premier cas, une erreur de 20 % sur l'évaluation des rejets pour un cnaillage de 60 mm n'apporterait qu'une modification de 3 % dans la production globale du stock. Ces premiers résultats sont en contradiction avec les conclusions habituelles des évaluations et il sera nécessaire de reprendre les calculs en modifiant le pas des classes de taille. Dans le deuxième cas, pour une diminution de L» et une augmentation de la vitesse de croissance, les variations de la production à terme restent à l'intérieur des fluctuations interannuelles habituelles. Dans tous les cas de figure, les calculs confirmant l'augmentation de la production à terme dans l'hypothèse d'une augmentation de maillage à 60 mm sont d'une robustesse à toute épreuve.

Droits : 1987 Ifremer
http://archimer.ifremer.fr/doc/1987/rapport-1763.pdf
http://archimer.ifremer.fr/doc/00000/1763/

Éditeur(s) : HAL CCSD
Résumé : Ctenophores are one of the four animal phyla positioned outside from the Bilateria. Most ctenophore species are planktonic and gelatinous, and are easily recognisable by their eight comb rows used for swimming. Ctenophore systematics remains nowadays poorly understood. Anatomical characters do not help much in placing them within the animal tree, and similarly the grounds for establishment of their internal phylogeny as well as delimitation of species are notoriously weak due to a paucity of informative morpho-anatomical characters. Until now, the use of molecular data has failed to improve significantly this situation. The aim of this PhD thesis was to bring a contribution to our understanding of ctenophore evolution at different taxonomic scales. At the metazoan level, the position of ctenophores was addressed using a phylogenomic approach. Taxonomic sampling was significantly improved through sequencing and assembly of transcriptomes of 22 non-bilaterian species (belonging to ctenophores, cnidarians and sponges). Two independent datasets were analysed, one consisting in an update of an existing supermatrix of 128 genes, the other one (4235 genes) having been entirely built de novo, thanks to a newly-devised semi-automated protocol intended to eliminate all major potential causes of artefacts (contaminations, paralogies, missing data). Results clearly contradict recent phylogenomic studies which claimed Ctenophora to be the most early-diverging animal lineage, our analyses instead supporting Porifera as the sister-group to other metazoans. The exact position of ctenophores remains however uncertain at this stage, different conditions of analyses yielding three contradictory hypotheses as open possibilities. At the intra-phyletic level, analyses of a ADNr 18S and ITS (internal transcribed spacers) dataset, together with study of duplicated genes and the results of phylogenomic analyses, allowed resolving most phylogenetic relationships between ctenophore orders and families, including the placement of the ctenophore tree root. Finally, at smaller taxonomic scale, in-depth comparison between two species of the genus Pleurobrachia using immunohistochemistry demonstrates the potential of these techniques for uncovering new structural “micro-anatomical” characters useful for diagnosis and identification of ctenophore species. It is our hope that this work will contribute to improving the systematics of a poorly known phylum of great importance for understanding early animal evolution.
Les cténophores représentent l’un des quatre embranchements animaux extérieurs aux Bilateria. La majoritédes espèces sont planctoniques et gélatineuses, et sont reconnaissables à leurs huit rangées de peignes dont lebattement permet la nage. Leur systématique est encore de nos jours mal comprise. L'anatomie descténophores offre peu de caractères aussi bien pour placer la lignée dans l’arbre des métazoaires, que pourétablir les relations de parenté au sein de l’embranchement et délimiter les espèces, et jusqu’à présent lesdonnées moléculaires n’ont pas permis de résoudre ces problèmes de manière satisfaisante. L’objectif de cetravail de thèse est de contribuer à améliorer notre compréhension de l'évolution des cténophores àdifférentes échelles taxonomiques. A l’échelle des métazoaires, la position phylogénétique des cténophores aété abordée par une approche phylogénomique. Un effort significatif a été réalisé pour améliorerl’échantillonnage taxonomique à travers le séquençage et l’assemblage des transcriptomes de 22 espèces denon-Bilateria (cténophores, cnidaires, spongiaires). Deux jeux de données indépendants ont été analysés, lepremier représentant une mise à jour d’une supermatrice existante de 128 gènes ; le second (4235 gènes)ayant été entièrement construit de novo via un protocole original comportant la mise au point de nouvellesméthodes pour traiter de manière semi-automatisée les principales sources potentielles d’artéfact(contaminations, paralogies, données manquantes). Les résultats contredisent certaines études récentes enmontrant que les spongiaires et non les cténophores représentent le groupe-frère des autres métazoaires. Laposition exacte de ces derniers reste à ce stade incertaine (trois options se présentant suivant les analyses). Al’échelle intra-phylétique, l'analyse d'un jeu de données comprenant les marqueurs ADNr 18S et InternalTranscribed Spacers (ITS), associée à des analyses de gènes dupliqués chez les cténophores et aux analysesphylogénomiques précédentes, a permis de résoudre une grande partie des relations phylogénétiques entre lesordres et les familles de cténophores, tout en permettant de préciser la position de la racine. Enfin, à uneéchelle taxonomique plus fine, une comparaison approfondie entre deux espèces du genre Pleurobrachia aumoyen de marquages immunohistochimiques montre le potentiel de ces techniques comme source denouveaux caractères structuraux « micro-anatomiques » à valeur diagnostique pour la délimitation etl’identification des espèces de cténophores. En conclusion, ce travail se veut une contribution au progrès dela systématique d’un embranchement encore méconnu et d’une grande importance pour la compréhensiondes évènements anciens de l’évolution animale.
https://tel.archives-ouvertes.fr/tel-01165014

NNT : 2014PA066448
tel-01165014
https://tel.archives-ouvertes.fr/tel-01165014
https://tel.archives-ouvertes.fr/tel-01165014/document
https://tel.archives-ouvertes.fr/tel-01165014/file/these_archivage_3158995o.pdf

Éditeur(s) : Université des Antilles Études caribéennes
Résumé : Ce numéro de la Revue Etudes Caribéennes consacré au tourisme vise à poser les bases d’une réflexion sur le fonctionnement des lieux touristiques, les programmes d’aménagement touristique et les différents enjeux pour les territoires. Lieu ouvert ou lieu fermé, flux concentré ou flux diffus, la nature du lieu touristique, avec ses pratiques, classiques ou originales, oriente les types d’impacts potentiels. Les choix opérés en matière d’aménagement touristique sont donc primordiaux : dans quelles conditions opte-t-on pour un tourisme balnéaire classique, massif, ou pour une approche touristique alternative ? Ces différentes déclinaisons sont-elles attachées à des territoires spécifiques ? Faut-il opposer ces deux approches ou les considérer complémentaires dans leurs environnements respectifs, peuvent-elles partager un même espace ? L’objectif de ce numéro thématique est de réunir des approches diverses et renouvelées qui développent des analyses sous l’angle de l’économie, de l’aménagement du territoire, de la sociologie, de la géographie… Les articles pourront proposer une réflexion théorique ou privilégier des études de cas, traitant des relations tourisme de masse – tourisme alternatif ou s’attachant à illustrer l’une de ces deux aspects. Les terrains privilégiés ne seront pas exclusivement caribéens et américains, ils pourront relever de n’importe quelle région du monde et illustrer des situations présentes et/ou passées. Les thématiques privilégiées seront : 1. Les concepts de tourisme de masse et de tourisme alternatif (identification, caractéristiques, logiques spatiales, enjeux économiques, sociaux, environnementaux). 2. Les lieux du tourisme masse (stations balnéaires, villes historiques, formes spécifiques d’aménagement des lieux et les types de structures d’accueil) 3. Alter-tourismes et renouvellement des pratiques touristiques (écotourisme, tourisme communautaire, agrotourisme …) 4. L’aménagement des lieux touristiques et les modalités de gestion des environnements protégés (conservation, protection et pratiques touristiques et récréatives) 5. Les enjeux économiques, sociaux, environnementaux autour de différents types de lieux touristiques. This issue of the Revue Etudes Caribéennes, devoted to tourism, aims to lay the foundations of a reflection on the functioning tourist places, their development programs and the different challenges presented to them. Exposed/Open or closed location, concentrated or diffused flows, the nature of these tourist places with their traditional or original customs, orientate different types of potential impacts. The choices made in tourism development are essential: in which conditions should be opted for the traditional seaside tourism, or for mass tourism, or for alternative tourism? Are certain properties linked to specific territories? Should we oppose these two approaches or consider them as complementary in their environments and can they share the same place? The objective of this thematic issue is to call for various and renewed approaches which develop analysis in terms of the economy, regional planning, sociology, geography etc. Articles may propose a theoretical reflection or focus on case studies, dealing with the relations of mass tourism, alternative tourism, or to illustrate one of these two aspects. Priorities fields will not exclusively be Caribbean and American but can come from any part of the world and can illustrate post and/or present situations. The prioritized analysis will include: 1. The concepts of mass tourism and alternative tourism (identification, characteristics, spatial logics, and economic, social and environmental issues). 2. Places of mass tourism (resorts, historic cities, specific forms of site layout and reception facilities) 3. Alter-tourism and the renewal of tourism practices (ecotourism, community tourism, agrotourism) 4. The development of tourist spots and of management methods concerning protected environments (conservation, protection, tourism practices and recreational practices) 5. Economic, social, and environmental issues surrounding different types of tourist places. Este número de la Revista Estudios Caribeños consagrado al turismo apunta a poner las bases de una reflexión sobre el funcionamiento de los lugares turísticos, los programas de ordenación turística y las diferentes apuestas para los territorios. Lugar abierto o cerrado, flujos concentrados o flujos difusos, la naturaleza del lugar turístico, con sus prácticas, clásicas u originales, orienta los tipos de impactos potenciales. Las elecciones operadas en materia de ordenación turística son primordiales: ¿en qué condiciones se opta por un turismo balneario clásico, masivo, o por un enfoque turístico alternativo? ¿Estas diferentes declinaciones están relacionadas a territorios específicos? ¿Se debe oponer estos dos enfoques o considerarlos complementarios en sus ambientes respectivos, pueden compartir el mismo espacio? El objetivo de este número temático es de reunir los diversos y renovados enfoques que desarrollan los análisis sobre el ángulo de la economía, la ordenación del territorio, la sociología, la geografía… Los artículos podrán proponer una reflexión teórica o privilegiar los estudios prácticos, tratando las relaciones turismo de masa-turismo alternativo o dirigirse a ilustrar uno de estos dos aspectos. Los terrenos privilegiados no serán exclusivamente caribeños y americanos, podrán hacer frente a cualquier región del mundo e ilustrar situaciones presentes y/o pasadas. Los temas privilegiados serán: 1. Los conceptos de turismo de masa y de turismo alternativo (identificación, características, lógicas espaciales, apuestas económicas, sociales, ambientales). 2. Los lugares de turismo de masa (estaciones balnearias, ciudades históricas, formas específicas de ordenación de lugares y de tipos de estructuras de recepción). 3. Alter-turismos y renovación de las prácticas turísticas (ecoturismo, turismo comunitario, agroturismo...). 4. La ordenación de lugares turísticos y las modalidades de gestión de los ambientes protegidos (conservación, protección y prácticas turísticas y recreativas). 5. Las apuestas económicas, sociales, ambientales alrededor de diferentes tipos de lugares turísticos.

Droits : info:eu-repo/semantics/openAccess
urn:doi:10.4000/etudescaribeennes.7521
http://journals.openedition.org/etudescaribeennes/7521

Éditeur(s) : Elsevier
Résumé : Genetic variation was surveyed at 11 microsatellite loci and at 517 bp of the mitochondrial control region to investigate the presence of genetic stock structure in swordfish (Xiphias gladius) in four proximal localities of the southwest Indian Ocean. One aim of this study was to serve as a preliminary examination for congruency of structure detected by these two genetic markers, prior to conducting a more comprehensive basin-wide survey of the Indian Ocean and nearby surrounding areas. Analyses of multilocus microsatellite genotypes and mitochondrial control region sequences both revealed a great homogeneity between samples. Genetic diversity detected at the regional scale was not significantly higher than detected at the local scale. Results suggest that the southwest Indian Ocean globally functions as a unique panmictic population. However, some discrete genetic differences appeared that could possibly indicate influence from a second genetic pool in the northern part of the Indian Ocean. This structure appeared to be sex-dependent with genetic differences higher among female than among male samples. This result may indicate a higher level of spawning area fidelity for females with a subsequent sampling bias tending to homogenise male genotypic distributions. (c) 2009 Elsevier B.V. All rights reserved.
Fisheries Research (0165-7836) (Elsevier), 2009-05 , Vol. 97 , N. 3 , P. 263-269

Droits : 2009 Elsevier B.V. All rights reserved.
http://archimer.ifremer.fr/doc/2009/publication-6455.pdf
DOI:10.1016/j.fishres.2009.03.004
http://archimer.ifremer.fr/doc/00000/6455/

Éditeur(s) : HAL CCSD Oxford University Press (OUP)
Résumé : International audience
Several studies, both theoretical and experimental, show that sedimentary rocks have a fractal pore-grain interface. In this paper a computer simulated 3-D sedimentary rock structure generated by the Relaxed Ballistic Bidisperse Deposition Model (RBBDM), is investigated to characterize the micro structure of its pores. The pore volume and the rock-pore interface show the same fractal dimension indicating that the pore volume is a fractal. The two point density correlation is computed for the pore space and the results compare favourably with the range reported from experiments. An array of 2-D X-ray tomography micrograph sections of a real sedimentary rock, an oolitic limestone (pure calcite) from the Mondeville formation of Middle Jurassic age (Paris Basin, France), was used to generate a 3-D bitmap. The 3-D real rock sample generated in this manner, was analysed for similar studies as the simulated structure. The results were compared with those obtained from simulation. The simulation results agree qualitatively with the real rock sample. Diffusion through the connected pore space of the simulated structure was studied using a random walk algorithm and the results compared with the similar simulation study done on the 3-D oolitic limestone specimen. In both cases diffusion was found to be anomalous indicating that the sedimentary rock has a fractal geometry. The favourable comparability of results between the simulated and real rock supports the usefulness of the model of sedimentary rock generation which can be applicable to transport phenomena.
ISSN: 0956-540X

hal-00823061
https://hal.archives-ouvertes.fr/hal-00823061
DOI : 10.1093/gji/ggs084

Éditeur(s) : Université Nice Sophia Antipolis
Résumé : The Lesser Antilles is a case study of a very slow subduction (~2 cm/yr) of an old oceanic lithosphere (~84-100 Ma). The region presents a relatively low seismic activity, especially along the interplate contact, and the seismic hazard associated with a possible mega-thrust earthquake is still poorly known. This PhD thesis is a first step toward assessing the ability of the Lesser Antilles subduction zone to produce such a large subduction event. To do so, it aims at constraining the downdip width of the interplate's seismogenic zone. The lack of coverage of permanent seismological stations is a major limitation in the exploration of the Lesser Antilles subduction zone. It is due to the presence of only small aligned islands at far distances from the potentially seismogenic interplate area. Several oceanographic cruises were therefore planned that notably allowed the repeated deployment of ocean bottom seismometers; some of them being left for a few months of background seismicity recording. This thesis specifically focuses on two sets of wide-angle seismic data acquired offshore the Dominica and Martinique islands. From their analysis 3D and 2D tomographic models were produced respectively over the forearc region and across the whole subduction complex. These models constrain the plates' seismic structure as well as their geometry. They allow the discussion of how the imaged structures affect the subduction processes and give a first estimation of the downdip width of the seimogenic zone, defined as the segment of the interplate between the backstop and the upper plate's Moho. The joint interpretation of seismic models and earthquake localizations then refine this first assessment. Epicenter distribution from height months of seismic recording shows indeed that seismicity concentrates within the inner forearc region. The strong velocity gradient that characterize its basement suggests it is denser and more rigid than the more deformable outer forearc basement. The updip limit of the seismogenic zone could then lie arcward of the backstop at the contact of the interplate and the seaward end of the inner forearc crust. At depth, interplate earthquake mechanisms are observed between 35 and 45 km depth and interpreted to have occurred at the downdip limit of the seismogenic zone. The latter could reach a depth over 10,km deeper than the contact of the upper plate's Moho with the interplate, and therefore lie within the mantle wedge. All together, these results imply a large downdip width of the seismogenic zone (~70 km) offshore the Dominica and Martinique islands. Further work is, however, needed in order to fully comprehend the ability of the Lesser Antilles subduction zone to produce a possible mega-thrust earthquake. This would necessitate the evaluation of seismic coupling at the interplate contact and the possible segmentation of the seismogenic zone, for instance, due to the subduction of oceanic ridges.
Les Petites Antilles présentent un contexte géodynamique caractérisé par la subduction à très faible vitesse (~2 cm/an) d'une lithosphère océanique âgée (~84-100 Ma). L'activité sismique y est relativement faible, notamment à l'interplaque, où l'aléa sismique lié à un éventuel séisme de méga-chevauchement reste encore mal contraint. Cette thèse se veut être une première étape dans l'évaluation de la capacité de la zone de subduction des Petites Antilles à générer un tel évènement. Dans ce but, ces travaux tentent d'appréhender l'extension en profondeur du domaine sismogène de l'interplaque. Le manque de couverture des stations sismologiques permanentes est un inconvénient majeur dans l'exploration des Petites Antilles. Il s'explique en raison du peu de terres émergées et de leur éloignement de la zone potentiellement sismogène de l’interplaque. La région a donc fait l'objet de plusieurs campagnes océanographiques qui ont permis, notamment, le déploiement de sismomètres fond de mer (OBS); certains instruments étant restés immergés plusieurs mois afin de procéder à une écoute de la sismicité. Les travaux réalisés au cours de cette thèse se sont focalisés sur deux jeux de données de sismique grand-angle acquis au large des îles de la Dominique et de la Martinique. Leur analyse a permis la construction de modèles tomographiques 3D et 2D respectivement à l'échelle de l'avant-arc et de l'ensemble de la subduction. Ces modèles renseignent sur la structure sismique des plaques en convergence ainsi que sur leur géométrie. Ils permettent de discuter le rôle de la structure dans le fonctionnement de la subduction et d’obtenir une première estimation de l'extension en profondeur de la zone sismogène en considérant la portion de l'interplaque comprise entre le butoir et le Moho de la plaque supérieure. Dans un second temps, l'interprétation conjointe des modèles tomographiques et des localisations des séismes locaux a permis d'affiner cette estimation. Huit mois d'enregistrement de la sismicité montrent en effet une concentration des épicentres dans la région interne de l’avant-arc. Celle-ci présente un socle épais, à fort gradient de vitesse interprété comme plus dense et rigide que le socle de la région externe de l'avant-arc, plus déformable. La limite amont de la zone sismogène pourrait donc se situer en retrait de la position du butoir au contact de l'interplaque et de la limite entre ces deux zones de l'avant-arc. En profondeur, des mécanismes interplaques sont observés entre 35 et 45 km et interprétés comme des marqueurs de la limite aval de la zone sismogène. Cette dernière pourrait donc atteindre une profondeur jusqu'à 10 km supérieure à la limite précédemment évoquée et se trouver, par conséquent, au contact du manteau lithosphérique. L'ensemble de ces résultats suggèrent une extension en profondeur de la zone sismogène (i.e une largeur) de près de 70 km face aux îles de la Dominique et de la Martinique. Ces travaux doivent cependant être poursuivis afin d’évaluer pleinement la capacité de la zone de subduction des Petites Antilles à générer un éventuel séisme de méga-chevauchement. Le taux de couplage à l'interplaque doit être précisé ainsi que sa possible segmentation en lien, par exemple, avec à l'entrée en subduction des rides océaniques.

Droits : info:eu-repo/semantics/openAccess
http://archimer.ifremer.fr/doc/00172/28335/26628.pdf
http://archimer.ifremer.fr/doc/00172/28335/