Éditeur(s) : HAL CCSD Wiley-Blackwell
Résumé : International audience
AIMS: Virus detection has often been difficult due to a low concentration in water. In this study, we developed a new procedure based on concentration of virus particles on an innovative support: poly-L-lysine dendrigrafts (DGL), coupled with directed nucleic acid extraction and real-time PCR quantification. METHODS AND RESULTS: This method was evaluated using the bacteriophage MS2 as a model virus. This virus exhibited the size and structural properties of human pathogenic enteric viruses and has often been used to assess new supports of concentration. Moreover, this bacteriophage is also a faecal contamination indicator. In this study, many water filtration conditions were tested (volume of water, concentration, etc.), and more than 80% of bacteriophage were recovered after filtration on polymer, in most conditions. We demonstrated that the method was linear (slope = 0*99 ± 0*04 and Y intercept when x = -0*02 ± 0*28), valid (as manipulators, tested concentrations, volumes of sample and batch of polymer did not have any influence on concentration) and sensitive (allowing to concentrate up to 16,600-fold 1 l of sample and to detect and quantify down to 750 GC l(-1) and 7500 GC l(-1), respectively). CONCLUSIONS: To conclude, this support exhibits high interest to retain viruses and to allow to detect low concentration of virus in water. SIGNIFICANCE AND IMPACT OF THE STUDY: This study gives valuable advance in the methods of concentration and diagnosis of virus in water.
Journal of Applied Microbiology

hal-00828987
https://hal.archives-ouvertes.fr/hal-00828987
DOI : 10.1111/jam.12209
PUBMED : 23551794

Éditeur(s) : HAL CCSD Elsevier
Résumé : Obduction processes, though spectacular (dense oceanic ophiolites are emplaced on top of light, continental rocks along thousands of km), have been little elucidated since the advent of plate tectonics. Based on convergence velocities and blueschist formation, we show that the two recent large-scale, Upper Jurassic and Upper Cretaceous obductions coincided with periods during which velocities increased abruptly and more than doubled. The latter obduction also modified the interplate coupling across adjacent subduction zones. We critically propose a mechanism in which large-scale obductions are triggered by intraplate instabilities resulting from sharp plate accelerations, possibly in response to superplume events
ISSN: 0012-821X

hal-00407960
https://hal.archives-ouvertes.fr/hal-00407960
DOI : 10.1016/j.epsl.2007.04.002

Éditeur(s) : HAL CCSD
Résumé : International audience
In the external Western Alps, the collision started with the shortening of the proximal European (Dauphinois) margin. One peculiar feature is the crustal thickening that finally led to the exhumation of the basement External Crystalline Massifs (ECM) during the Neogene. In these massifs, various age of shortening, structural style and sequence of deformation have been proposed leading to different kinematics along the Alpine arc that in turn do not imply the same crustal/lithospheric strength. In this contribution, we present new structural and microstructural data along with Ar/Ar ages from field studies in the Oisans-Grandes Rousses massifs, between Grenoble and Briançon). From balanced cross-sections, we show that about half of the external zone crustal shortening is accommodated within the ECM with similar Ar/Ar ages in all massifs. Furthermore, the shear zones present very large thicknesses (several hundred meters) and do not reactivated either the inherited Liassic normal faults or the Variscan foliation. Finally, the main shortening structures are localized beneath inherited Liassic syn-rift basins. These results suggest that the crust was weak during its shortening. Such behavior is most likely due to the combination of several factors: the crust burial (below the overthrusting internal units) down to about 10km, the presence of weak inherited syn-rift basins, and the strain softening in shear zones that evolve in mica-rich phyllonites. These results are consistent with the studies in the Aar massif and should most likely apply to the Mont Blanc massif, whose relationships with its cover are still debated.
EGU General Assembly
Vienne, Austria

insu-00839426
https://hal-insu.archives-ouvertes.fr/insu-00839426
BIBCODE : 2013EGUGA..15.8837B

Éditeur(s) : HAL CCSD Public Library of Science
Résumé : International audience
The extinct Odontopterygiformes are the sole birds known to possess strong and sharp bony pseudoteeth, the shape and location of which are closely mimetic of real teeth. The structure of the pseudoteeth is investigated here in a late Pliocene/early Pleistocene species, Pelagornis mauretanicus, using X-ray microtomography and thin sections. The results are interpreted with regard to the pseudotooth mode of growth, and have implications concerning aspects of Pelagornis ecology. The larger pseudoteeth are hollow and approximately cone-shaped, and the smaller ones are rostro-caudally constricted. The walls of pseudoteeth are composed of bone tissue of the fibro-lamellar type, which is intensively remodeled by Haversian substitution. The jaw bones display the same structure as the pseudoteeth, but their vascular canals are oriented parallel to the long axis of the bones, whereas they are perpendicular to this direction in the pseudoteeth. There is no hiatus or evidence of a fusion between the pseudoteeth and the jaw bones. Two possible models for pseudotooth growth are derived from the histological data. The most plausible model is that pseudotooth growth began after the completion of jaw bone growth, as a simple local protraction of periosteal osteogenic activity. Pseudotooth development thus occurred relatively late during ontogeny. The highly vascularized structure and the relative abundance of parallel-fibered bone tissue in the pseudoteeth suggest poor mechanical capabilities. The pseudoteeth were most likely covered and protected by the hardened, keratinized rhamphotheca in the adult during life. The late development of the pseudoteeth would involve a similarly late and/or partial hardening of the rhamphotheca, as displayed by extant Anseriformes, Apterygiformes and some Charadriiformes. This would add support to the hypothesis of a close phylogenetic relationship between Odontopterygiformes and Anseriformes. The late maturation of the Pelagornis feeding apparatus, and hence the delayed capability for efficient prey catching, suggests that Pelagornis was altricial.
ISSN: 1932-6203

hal-01545486
https://hal.archives-ouvertes.fr/hal-01545486
DOI : 10.1371/journal.pone.0080372

Éditeur(s) : HAL CCSD Cambridge University Press (CUP)
Résumé : International audience
Andros is a key island for understanding both the timing of high-pressure–low-temperature(HP-LT) metamorphism and the dynamics of crustal-scale detachment systems exhuming high-gradeunits in the Cyclades (Greece). Using phengite 40Ar–39Ar geochronology coupled with thermobarometry,as well as data from literature, we constrain the pressure–temperature–time (P-T-t) paths of theMakrotantalon and Attic–Cycladic Blueschist units on Andros. Peak conditions of the HP-LT episodein the Makrotantalon unit are 550 °C and 18.5 kbar, dated at 116 Ma. We correlate this episode withEarly Cretaceous blueschist facies metamorphism recognized in the Pelagonian zone of continentalGreece. This is a new argument favouring a Pelagonian origin for the Makrotantalon unit. In the Attic–Cycladic Blueschist unit, the P-T-t path is characterized by: (1) exhumation after peak conditions inHP-LT conditions between 55 and 35 Ma; (2) isobaric heating at 7 kbar until 30 Ma; and (3) isothermaldecompression until 21 Ma. This thermal evolution and timing are similar to those of the neighbouringTinos Island, emphasizing major thermal re-equilibration at the transition between stable and retreatingsubduction. Modifications of the crustal thermal state played a major role in the evolution of the NorthCycladic Detachment System, below which Andros HP-LT units were exhumed.
ISSN: 0016-7568

insu-01114909
https://hal-insu.archives-ouvertes.fr/insu-01114909
DOI : 10.1017/S0016756814000661

Éditeur(s) : HAL CCSD Centers for Disease Control and Prevention
Résumé : International audience
ISSN: 1080-6040

hal-01447667
https://hal.archives-ouvertes.fr/hal-01447667
DOI : 10.3201/eid2210.160052

Éditeur(s) : HAL CCSD Public Library of Science
Résumé : International audience
The Hedgehog (Hh) family of secreted proteins act as morphogens to control embryonic patterning and development in a variety of organ systems. Post-translational covalent attachment of cholesterol and palmitate to Hh proteins are critical for multimerization and long range signaling potency. However, the biological impact of lipid modifications on Hh ligand distribution and signal reception in humans remains unclear. In the present study, we report a unique case of autosomal recessive syndromic 46,XY Disorder of Sex Development (DSD) with testicular dysgenesis and chondrodysplasia resulting from a homozygous G287V missense mutation in the hedgehog acyl-transferase (HHAT) gene. This mutation occurred in the conserved membrane bound O-acyltransferase (MBOAT) domain and experimentally disrupted the ability of HHAT to palmitoylate Hh proteins such as DHH and SHH. Consistent with the patient phenotype, HHAT was found to be expressed in the somatic cells of both XX and XY gonads at the time of sex determination, and Hhat loss of function in mice recapitulates most of the testicular, skeletal, neuronal and growth defects observed in humans. In the developing testis, HHAT is not required for Sertoli cell commitment but plays a role in proper testis cord formation and the differentiation of fetal Leydig cells. Altogether, these results shed new light on the mechanisms of action of Hh proteins. Furthermore, they provide the first clinical evidence of the essential role played by lipid modification of Hh proteins in human testicular organogenesis and embryonic development.
ISSN: 1553-7390

hal-01063929
https://hal.archives-ouvertes.fr/hal-01063929
DOI : 10.1371/journal.pgen.1004340
PUBMED : 24784881

Éditeur(s) : HAL CCSD Lippincott, Williams & Wilkins
Résumé : International audience
OBJECTIVES: The ANRS-112 INTERPRIM trial assessed whether fixed-cycles of antiretroviral treatment interruption (ART-STI) combined or not with pegylated interferon alpha-2b (peg-IFN) could lower viral load and achieve a healthier immune system in patients diagnosed during primary HIV-1-infection (PHI). DESIGN AND METHODS: Patients were randomized to receive either continuous ART (cART) during 72 weeks, or cART during 36 weeks followed by three ART-STIs, or the same ART-STIs associated with peg-IFN during the first 14 weeks and each interruption (ART-STI-IFN). Treatment was stopped at week 72. Final evaluation was based on plasma HIV-RNA level 6 months after the last treatment interruption. RESULTS: Eighty-seven percent of patients achieved undetectable HIV-RNA at week 32, with no deleterious impact of sequential treatment interruptions (STIs). Viral rebounds during interruptions were lower in the ART-STI-IFN than in the ART-STI group and during the second and third interruptions compared with the first one. However, HIV-RNA levels, CD4 T-cell counts and CD4 T/CD8 T ratios were similar between groups after the 6-month interruption, with a persistent effect on CD4 T cells and total cell-associated HIV-DNA levels. Predictive factors of virological outcome were HIV-RNA and HIV-DNA levels at PHI and HIV-DNA levels at treatment interruption. HIV-specific responses did not differ between strategies and were not associated with outcome. Forty-eight percent of patients experienced treatment resumption during long-term follow-up without difference between groups. CONCLUSION: When initiated during PHI, STIs associated or not with IFN did not result in a different outcome as compared to cART. All regimens showed a high response rate and a sustained immunological benefit after cessation.
ISSN: 0269-9370

hal-00875175
https://hal.archives-ouvertes.fr/hal-00875175
DOI : 10.1097/QAD.0b013e32835844d9
PUBMED : 22842994

Éditeur(s) : HAL CCSD Wiley-Blackwell
Résumé : International audience
Virus detection has often been difficult due to a low concentration in water. In this study, we developed a new procedure based on concentration of virus particles on an innovative support: poly-L-lysine dendrigrafts (DGL), coupled with directed nucleic acid extraction and real-time PCR quantification. Methods and Results: This method was evaluated using the bacteriophage MS2 as a model virus. This virus exhibited the size and structural properties of human pathogenic enteric viruses and has often been used to assess new supports of concentration. Moreover, this bacteriophage is also a faecal contamination indicator. In this study, many water filtration conditions were tested (volume of water, concentration, etc.), and more than 80% of bacteriophage were recovered after filtration on polymer, in most conditions. We demonstrated that the method was linear (slope = 0 99 0 04 and Y intercept when x = 0 02 0 28), valid (as manipulators, tested concentrations, volumes of sample and batch of polymer did not have any influence on concentration) and sensitive (allowing to concentrate up to 16 600-fold 1 l of sample and to detect and quantify down to 750 GC l 1 and 7500 GC l 1, respectively). Conclusions: To conclude, this support exhibits high interest to retain viruses and to allow to detect low concentration of virus in water. Significance and Impact of the Study: This study gives valuable advance in the methods of concentration and diagnosis of virus in water.
Journal of Applied Microbiology

hal-00932328
https://hal.archives-ouvertes.fr/hal-00932328
DOI : 10.1111/jam.12209

Éditeur(s) : HAL CCSD Paris : Muséum national d'histoire naturelle
Résumé : International audience
The chromosomal diversity within six species of Mormyridae belonging to six different genera (Gnathonemus petersii, Marcusenius moorii, Ivindomyrus obdenboschi, Brienomyrus sp., Stomatorhinus walkeri and Petrocephalus microphthalmus) is investigated for the first time. These species have a conserved diploid chromosome number of 2n = 50 (except G. petersii 2n = 48) but different chromosome formulae, essentially involving pericentric inversions. C-banding has been used for the localization of heterochromatin. For a deeper interspecific comparison, several molecular markers were localized in the chromosomes by fluorescence in situ hybridization (FISH): a 18S rDNA probe; a microsatellite probe (GAA)(10), and a telomeric probe (TTAGGG)(7). The distribution of the 18S rDNA patterns, in particular, shows an amazing specific diversity, confirming a rapid radiation accompanied by major chromosome rearrangements.
ISSN: 0399-0974

hal-01544756
https://hal.archives-ouvertes.fr/hal-01544756

Éditeur(s) : HAL CCSD American Medical Association (AMA)
Résumé : International audience
IMPORTANCE Although frequently used in treating intensive care unit (ICU) patients with sepsis, empirical antifungal therapy, initiated for suspected fungal infection, has not been shown to improve outcome. OBJECTIVE To determine whether empirical micafungin reduces invasive fungal infection (IFI)-free survival at day 28. DESIGN, SETTING, AND PARTICIPANTS Multicenter double-blind placebo-controlled study of 260 nonneutropenic, nontransplanted, critically ill patients with ICU-acquired sepsis, multiple Candida colonization, multiple organ failure, exposed to broad-spectrum antibacterial agents, and enrolled between July 2012 and February 2015 in 19 French ICUs. INTERVENTIONS Empirical treatment with micafungin (100mg, once daily, for 14 days) (n = 131) vs placebo (n = 129). MAIN OUTCOMES AND MEASURES The primary end pointwas survival without proven IFI 28 days after randomization. Key secondary end points included new proven fungal infections, survival at day 28 and day 90, organ failure, serum (1-3)-beta-D-glucan level evolution, and incidence of ventilator-associated bacterial pneumonia. RESULTS Among 260 patients (mean age 63 years; 91 [35%] women), 251 (128, micafungin group; 123, placebo group) were included in the modified intent-to-treat analysis. Median values were 8 for Sequential Organ Failure Assessment (SOFA) score, 3 for number of Candida-colonized sites, and 99 pg/mL for level of (1-3)-beta-D-glucan. On day 28, there were 82 (68%) patients in the micafungin group vs 79 (60.2%) in the placebo group who were alive and IFI free (hazard ratio [HR], 1.35 [95% CI, 0.87-2.08]). Results were similar among patients with a (1-3)-beta-D-glucan level of greater than 80 pg/mL (n = 175; HR, 1.41 [95% CI, 0.85-2.33]). Day-28 IFI-free survival in patients with a high SOFA score (> 8) was not significantly different when compared between the micafungin vs placebo groups (HR, 1.69 [95% CI, 0.96-2.94]). Use of empirical micafungin decreased the rate of new invasive fungal infection in 4 of 128 patients (3%) in the micafungin group vs placebo (15/123 patients [12%]) (P =.008). CONCLUSIONS AND RELEVANCE Among nonneutropenic critically ill patients with ICU-acquired sepsis, Candida species colonization at multiple sites, and multiple organ failure, empirical treatment with micafungin, compared with placebo, did not increase fungal infection-free survival at day 28.
ISSN: 0098-7484

hal-01405822
https://hal-univ-rennes1.archives-ouvertes.fr/hal-01405822
DOI : 10.1001/jama.2016.14655
PUBMED : 27706483

Éditeur(s) : HAL CCSD Nature Publishing Group
Résumé : International audience
Transmembrane CD95L (Fas ligand) can be cleaved to release a promigratory soluble ligand, cl-CD95L, which can contribute to chronic inflammation and cancer cell dissemination. The motility signaling pathway elicited by cl-CD95L remains poorly defined. Here, we show that in the presence of cl-CD95L, CD95 activates the Akt and RhoA signaling pathways, which together orchestrate an allosteric activation of the Na+/H+ exchanger NHE1. Pharmacologic inhibition of Akt or ROCK1 independently blocks the cl-CD95L-induced migration. Confirming these pharmacologic data, disruption of the Akt and ROCK1 phosphorylation sites on NHE1 decreases cell migration in cells exposed to cl-CD95L. Together, these findings demonstrate that NHE1 is a novel molecular actor in the CD95 signaling pathway that drives the cl-CD95L-induced cell migration through both the Akt and RhoA signaling pathways.
ISSN: 2045-2322

hal-01334067
https://hal-univ-rennes1.archives-ouvertes.fr/hal-01334067
https://hal-univ-rennes1.archives-ouvertes.fr/hal-01334067/document
https://hal-univ-rennes1.archives-ouvertes.fr/hal-01334067/file/Cleaved%20FAS%20ligand%20CC-BY.pdf
DOI : 10.1038/srep28008

Éditeur(s) : HAL CCSD Frontiers Media
Résumé : International audience
With global climate change altering marine ecosystems, research on plankton ecology is likely to navigate uncharted seas. Yet, a staggering wealth of new plankton observations, integrated with recent advances in marine ecosystem modelling, may shed light on marine ecosystem structure and functioning. A EuroMarine foresight workshop on the “Impact of climate change on the distribution of plankton functional and phylogenetic diversity” (PlankDiv) identified five grand challenges for future plankton diversity and macroecology research: 1) What can we learn about plankton communities from the new wealth of high-throughput ‘omics’ data? 2) What is the link between plankton diversity and ecosystem function? 3) How can species distribution models be adapted to represent plankton biogeography? 4) How will plankton biogeography be altered due to anthropogenic climate change? and 5) Can a new unifying theory of macroecology be developed based on plankton ecology studies? In this review, we discuss potential future avenues to address these questions, and challenges that need to be tackled along the way.
ISSN: 2296-7745

hal-01545318
https://hal.archives-ouvertes.fr/hal-01545318
DOI : 10.3389/fmars.2017.00068

Éditeur(s) : HAL CCSD Elsevier
Résumé : International audience
The deep structure and deformation mechanisms of the Longmen Shan thrust belt (Sichuan, China), at the eastern border of the Tibetan plateau, were largely debated after the devastating Mw 7.9 Wenchuan earthquake (2008). Recent geophysical studies and field investigations have been focused on the active motion of the major Beichuan fault, which ruptured during the earthquake. However, the total exhumation across the fault still remains unclear. In the hanging wall of the Beichuan fault, the South China block is exhumed in the Pengguan massif. Close to the Beichuan fault, the rocks of the Pengguan massif underwent greenschist facies metamorphism associated with brittle-ductile deformation. No metamorphism is observed in the footwall of the fault. In this study, we characterize and date the metamorphic history recorded in the hanging wall of the Beichuan fault in order to constrain the depth and timing of exhumation of the rocks of the Pengguan massif along the fault. A high-resolution petrological approach involving chemical analyses and X-ray maps was used to analyze the micrometric metamorphic minerals. The P-T conditions of the greenschist facies metamorphic event were estimated by an inverse multi-equilibrium thermodynamic approach. The results, 280 ± 30 °C and 7 ± 1 kbar, suggest that the rocks of the Pengguan massif were exhumed from ca. 20 km depth. Our results underline the importance of the thrusting component in the long-term behavior of the Beichuan fault and provide a minimal depth at which the fault is rooted. In situ laser ablation 40Ar/39Ar dating of metamorphic white mica revealed that the greenschist overprint occurred at 135–140 Ma.The Pengguan massif was therefore partially thrusted along the Beichuan fault during the Lower Cretaceous, long before the Eocene-Miocene exhumation phase which is well-constrained by low-temperature thermochronology. Our results provide the first independent depth information for the exhumation history of the Pengguan massif and reveal a previously undocumented Lower Cretaceous tectonic event that marks the onset of the thick-skinned deformation in the external domain of the Longmen Shan (East of the Wenchuan Fault).
ISSN: 0743-9547

hal-01685556
https://hal.archives-ouvertes.fr/hal-01685556
DOI : 10.1016/j.jseaes.2017.04.003

Éditeur(s) : HAL CCSD Public Library of Science
Résumé : International audience
RATIONALE: Optimal management of complicated parapneumonic effusions (CPPE) remains controversial. OBJECTIVES: to assess safety and efficacy of iterative therapeutic thoracentesis (ITTC), the first-line treatment of CPPE in Rennes University Hospital. METHODS: Patients with CPPE were identified through our computerized database. We retrospectively studied all cases of CPPE initially managed with ITTC in our institution between 2001 and 2010. ITTC failure was defined by the need for additional treatment (i.e. surgery or percutaneous drainage), or death. RESULTS: Seventy-nine consecutive patients were included. The success rate was 81% (n = 64). Only 3 patients (4%) were referred to thoracic surgery. The one-year survival rate was 88%. On multivariate analysis, microorganisms observed in pleural fluid after Gram staining and first thoracentesis volume ≥450 mL were associated with ITTC failure with adjusted odds-ratios of 7.65 [95% CI, 1.44-40.67] and 6.97 [95% CI, 1.86-26.07], respectively. The main complications of ITTC were iatrogenic pneumothorax (n = 5, 6%) and vasovagal reactions (n = 3, 4%). None of the pneumothoraces required chest tube drainage, and no hemothorax or re-expansion pulmonary edema was observed. CONCLUSIONS: Although not indicated in international recommendations, ITTC is safe and effective as first-line treatment of CPPE, with limited invasiveness.
ISSN: 1932-6203

hal-00945622
https://hal-univ-rennes1.archives-ouvertes.fr/hal-00945622
https://hal-univ-rennes1.archives-ouvertes.fr/hal-00945622/document
https://hal-univ-rennes1.archives-ouvertes.fr/hal-00945622/file/fetchObject.pdf
PUBMED : 24400113

Éditeur(s) : HAL CCSD Wiley
Résumé : International audience
1. The Before-After Control-Impact Paired Series (BACIPS) design distinguishes natural spatial and temporalvariability from variation induced by an environmental impact (or intervention) of interest. BACIPS is a powerfultool to derive inferences about interventions when classic experimental approaches (e.g. which rely on spatialreplicates and random assignment of treatments) are not feasible or desirable. Previously applied BACIPSdesigns generally assume that effects are sudden, constant and long-lived: that is, that systems exhibit ‘stepchanges’in response to interventions. However, complex ecological interactions or gradual interventions maycreate delayed and/or progressive responses, potentially impeding the reliability of classic (step-change) analyses.2. We propose a novel approach, the Progressive-Change BACIPS, which generalizes and expands the scope ofBACIPS analyses.We evaluate the relative performance of this approach using both simulated and real data thatexhibit step-change, linear, asymptotic and sigmoid responses following an intervention.We quantify the statisticalpower and accuracy of the Progressive-Change BACIPS under varying initial population densities, intensityof spatial sampling, effect sizes and number of sampling dates After the intervention.3. We show that Progressive-Change BACIPS identified the correct model among the set of candidate modelsunder most conditions and led to accurate estimates of the parameters that were used to generate the simulateddata. When data were sparse, and the dynamics complex, simpler (more parsimonious) models were favouredover the more complex models that actually generated the simulated data. Application of the Progressive-Change BACIPS to existing data sets from the literature led to strong support for specific models (over alternatives)and led to more specific inferences than possible under the classic BACIPS approach.4. The Progressive-Change BACIPS proposed here is more flexible than the original BACIPS formulationbecause the data are used to inform the form of the final model, rather than having the form of the modelimposed on the data. This leads to better estimates of the effects of environmental impacts and the time-scalesover which they operate. As a result, the Progressive-Change BACIPS should be applicable to a wide range ofstudies and should help improve investigation of time-dependent effects. R code to perform Progressive-ChangeBACIPS analysis is provided.
ISSN: 2041-210X

hal-01416668
https://hal-univ-perp.archives-ouvertes.fr/hal-01416668
DOI : 10.1111/2041-210X.12655

Éditeur(s) : HAL CCSD Public Library of Science
Résumé : International audience
Over the last few decades, sessile benthic organisms from the Mediterranean Sea have suffered from the global warming of the world's oceans, and several mass mortality events were observed during warm summers. It has been hypothesized that mortality could have been due to a nutrient (food) shortage following the stratification of the water column. However, the symbiotic gorgonian Eunicella singularis has also presented a locally exceptional mortality, despite its autotrophic capacities through the photosynthesis of its dinoflagellate symbionts. Thus, this study has experimentally investigated the response of E. singularis to a thermal stress (temperature increase from 18 to 26 degrees C), with colonies maintained more than 2 months under four nutritional diets: autotrophy only (AO), autotrophy and inorganic nitrogen addition (AN), autotrophy and heterotrophy (AH), heterotrophy only (HO). At 18 degrees C, and contrary to many other anthozoans, supplementation of autotrophy with either inorganic nitrogen or food (heterotrophy) had no effect on the rates of respiration, photosynthesis, as well as in the chlorophyll, lipid and protein content. In the dark, heterotrophy maintained the gorgonian's metabolism, except a bleaching (loss of pigments), which did not affect the rates of photosynthesis. At 24 degrees C, rates of respiration, and photosynthesis significantly decreased in all treatments. At 26 degrees C, in addition to a decrease in the lipid content of all treatments, a bleaching was observed after 1 week in the AO treatment, while the AH and AN treatments resisted three weeks before bleaching. These last results suggest that, temperatures above 24 degrees C impair the energetic reserves of this species and might explain the mortality events in the Mediterranean.
ISSN: 1932-6203

hal-01546169
https://hal.archives-ouvertes.fr/hal-01546169
DOI : 10.1371/journal.pone.0064370

Éditeur(s) : HAL CCSD Le Cherche midi CNRS
Résumé : 192 pages
National audience
https://hal.archives-ouvertes.fr/hal-01118878

ISBN : 978-2-7491-4075-9
hal-01118878
https://hal.archives-ouvertes.fr/hal-01118878

Éditeur(s) : HAL CCSD Cambridge University Press (CUP)
Résumé : International audience
This paper presents a synthetic view of the geodynamic evolution of the Zagros orogen within the frame of the Arabia-Eurasia collision. The Zagros orogen and the Iranian plateau preserve a record of the long-standing convergence history between Eurasia and Arabia across the Neo-Tethys, from subduction/obduction processes to present-day collision (from ~ 150 to 0 Ma). We herein combine the results obtained on several geodynamic issues, namely the location of the oceanic suture zone, the age of oceanic closure and collision, the magmatic and geochemical evolution of the Eurasian upper plate during convergence (as testified by the successive Sanandaj-Sirjan, Kermanshah and Urumieh-Dokhtar magmatic arcs), the P-T-t history of the few Zagros blueschists, the convergence characteristics across the Neo-Tethys (kinematic velocities, tomographic constraints, subduction zones and obduction processes), together with a survey of recent results gathered by others. We provide lithospheric-scale reconstructions of the Zagros orogen from ~ 150 to 0 Ma across two SW-NE transects. The evolution of the Zagros orogen is also compared to those of the nearby Turkish and Himalayan orogens. In our geotectonic scenario for the Zagros convergence, we outline three main periods/regimes: (1) the Mid to Late Cretaceous (115-85 Ma) corresponds to a distinctive period of perturbation of subduction processes and interplate mechanical coupling marked by blueschist exhumation and upper-plate fragmentation, (2) the Paleocene-Eocene (60-40 Ma) witnesses slab break-off, major shifts in arc magmatism and distributed extension within the upper plate, and (3) from the Oligocene onwards (~ 30-0 Ma), collision develops with a progressive SW migration of deformation and topographic build-up (Sanandaj-Sirjan Zone: 20-15 Ma, High Zagros: ~12-8 Ma; Simply Folded Belt: 5-0 Ma) and with partial slab tear at depths (~10 Ma to present). Our reconstructions underline the key role played by subduction throughout the whole convergence history. We finally stress that such a long-lasting subduction system with changing boundary conditions also makes the Zagros orogen an ideal natural laboratory for subduction processes.
ISSN: 0016-7568

insu-00616582
https://hal-insu.archives-ouvertes.fr/insu-00616582
https://hal-insu.archives-ouvertes.fr/insu-00616582/document
https://hal-insu.archives-ouvertes.fr/insu-00616582/file/692.full.pdf
DOI : 10.1017/S001675681100046X

Éditeur(s) : HAL CCSD Cambridge University Press (CUP)
Résumé : International audience
ISSN: 1431-9276

hal-01680427
https://hal.univ-antilles.fr/hal-01680427
DOI : 10.1017/S1431927614010654