Éditeur(s) : HAL CCSD Public Library of Science
Résumé : International audience
The use of recombinant antigens has been shown to improve both the sensitivity and the standardization of the serological diagnosis of Farmer's lung disease (FLD). The aim of this study was to complete the panel of recombinant antigens available for FLD serodiagnosis with antigens of Lichtheimia corymbifera, known to be involved in FLD. L. corymbifera proteins were thus separated by 2D electrophoresis and subjected to western blotting with sera from 7 patients with FLD and 9 healthy exposed controls (HEC). FLD-associated immunoreactive proteins were identified by mass spectrometry based on a protein database specifically created for this study and subsequently produced as recombinant antigens. The ability of recombinant antigens to discriminate patients with FLD from controls was assessed by ELISA performed with sera from FLD patients (n = 41) and controls (n = 43) recruited from five university hospital pneumology departments of France and Switzerland. Forty-one FLD-associated immunoreactive proteins from L. corymbifera were identified. Six of them were produced as recombinant antigens. With a sensitivity and specificity of 81.4 and 77.3% respectively, dihydrolipoyl dehydrogenase was the most effective antigen for discriminating FLD patients from HEC. ELISA performed with the putative proteasome subunit alpha type as an antigen was especially specific (88.6%) and could thus be used for FLD confirmation. The production of recombinant antigens from L. corymbifera represents an additional step towards the development of a standardized ELISA kit for FLD diagnosis. © 2016 Rognon et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
ISSN: 1932-6203

hal-01367148
https://hal-univ-rennes1.archives-ouvertes.fr/hal-01367148
DOI : 10.1371/journal.pone.0160888

Éditeur(s) : Ministerio de Recursos Naturales Ministerio de Recursos Naturales ( El Zamorano, Honduras )
Résumé : (Bibliography) Includes bibliographical references (p. 68-71).
(Language) Text in Spanish and English.
Honduras
Honduras
Honduras
Honduras

433158232

Éditeur(s) : London : printed by Richard Taylor
Résumé : 1823
Siècle(s) traité(s) : 19
Droits : Domaine public
Provenance : Ville de Pointe-à-Pitre. Réseau des bibliothèques
Permalien : http://www.manioc.org/patrimon/PAP11212
PAP11212

Résumé : Item received on 11/27/2010
Panama -- Central America -- Panama Canal Zone

Droits : Public Domain Presumed (e.g. expiry of copyright term): This item is presumed to be in the public domain. The University of Florida George A. Smathers Libraries respect the intellectual property rights of others and do not claim any copyright interest in this item. Users of this work have responsibility for determining copyright status prior to reusing, publishing or reproducing this item for purposes other than what is allowed by fair use or other copyright exemptions. Any reuse of this item in excess of fair use or other copyright exemptions requires permission of the copyright holder.
2003.088.003.002
http://ufdc.ufl.edu/PCMI008756/00001

Éditeur(s) : Paris : Marpon et Flammarion
Siècle(s) traité(s) : 19
Provenance : Archives départementales de Guadeloupe
Permalien : http://www.manioc.org/patrimon/ADG18176
ADG18176

Éditeur(s) : HAL CCSD Wiley-VCH Verlag
Résumé : International audience
Saccharopolyspora rectivirgula is the principal cause of farmer's lung disease (FLD). Serodiagnosis is based on immunoprecipitation techniques or enzyme immunoassays with homemade crude antigens and is not standardized. We aimed to produce specific recombinant antigens for the development of a standardized ELISA. We recruited 41 patients and 43 healthy exposed controls from five university hospital pneumology departments in France and Switzerland. S. rectivirgula proteins were extracted, separated by 2D electrophoresis, and subjected to Western blotting, with sera from FLD patients or controls. FLD-specific proteins were identified by MS and were produced as recombinant antigens. The diagnostic performance of ELISA tests using the recombinant antigens was assessed with all the sera from FLD patients and controls. We identified 25 FLD-specific proteins, some of which play important roles in transport, nutrition, or virulence. We produced 17 of these proteins as recombinant antigens and assessed their suitability for inclusion in the ELISA test. A combination of three of these proteins (SR1FA, SR17, and SR22) proved remarkably effective at discriminating between patients and controls, with a sensitivity of 83% and a specificity of 77%. The recombinant antigens produced in this study constitute a major step toward the improvement of diagnostic performance and the standardization of FLD serodiagnosis.
ISSN: 1862-8346

hal-01115880
https://hal.archives-ouvertes.fr/hal-01115880
DOI : 10.1002/prca.201400024
PUBMED : 25264606

Éditeur(s) : Agricultural Experiment Station, University of the Virgin Islands Agricultural Experiment Station, University of the Virgin Islands ( Kingshill St. Croix, Kingshill St. Croix )
Résumé : (Statement of Responsibility) by Martin B. Adjei and Olasee Davis.
Cover title.
"September 1999."
United States Virgin Islands
Virgin Islands

46953696

Éditeur(s) : Paris : Imprimerie nationale
Siècle(s) traité(s) : 18
Droits : Domaine public
Provenance : Collectivité territoriale de Martinique. Bibliothèque Schoelcher
Permalien : http://www.manioc.org/patrimon/SCH13021
SCH13021

Éditeur(s) : Workshop held in Travinh (Vietnam), 5-8 March 2002
Résumé : The economie performance of shrimp rearing is govemed by three types of factors a) the environmental conditions prevailing around the farms, b) farmers' zootechnical practise and know how, c) economie management. The Gambas project aims at identifying the relative role played by these parameters in the success or failure of small-scale shrimp farming enterprises of the Mekong delta in Vietnam. It is being conducted by French and Vietnamese partners under the co-leadership of Ifremer and the Nha Trang Institute of Oceanography.

Droits : info:eu-repo/semantics/openAccess
http://archimer.ifremer.fr/doc/00083/19387/16981.pdf
http://archimer.ifremer.fr/doc/00083/19387/

Éditeur(s) : [S.l.] : L.G. Stahl
Siècle(s) traité(s) : 20
Droits : Domaine public
Provenance : Archives départementales de Guadeloupe
Permalien : http://www.manioc.org/patrimon/ADG18107
ADG18107

Éditeur(s) : CIMMYT CIMMYT ( México D.F )
Résumé : (Bibliography) Includes bibliographies.
(Statement of Responsibility) Derek Byerlee y Michael Collinson.
Translation of: Planning technologies appropriate to farmers (1980).
Also published in French.
Mexico

77018633

Éditeur(s) : London : J. Johnson, St. Paul's Church Yard & J. Edwards
Siècle(s) traité(s) : 18
Droits : Domaine public
Provenance : Collectivité territoriale de Martinique. Bibliothèque Schoelcher
Permalien : http://www.manioc.org/patrimon/SCH13243
SCH13243

Éditeur(s) : London : John Murray
Siècle(s) traité(s) : 19
Droits : Domaine public
Provenance : Ville de Pointe-à-Pitre. Réseau des bibliothèques
Permalien : http://www.manioc.org/patrimon/PAP11051
PAP11051

Éditeur(s) : Editorial Universitaria Editorial Universitaria ( San José )
Résumé : (Bibliography) Bibliographical footnotes.
(Funding) Secciâon Tesis de grado y ensayos ;
Costa Rica
Costa Rica

000134757
26639286
AAQ0806

Éditeur(s) : HAL CCSD Public Library of Science
Résumé : International audience
The use of recombinant antigens has been shown to improve both the sensitivity and the standardization of the serological diagnosis of Farmer's lung disease (FLD). The aim of this study was to complete the panel of recombinant antigens available for FLD serodiagnosis with antigens of Lichtheimia corymbifera, known to be involved in FLD. L. corymbifera proteins were thus separated by 2D electrophoresis and subjected to western blotting with sera from 7 patients with FLD and 9 healthy exposed controls (HEC). FLD-associated immunoreactive proteins were identified by mass spectrometry based on a protein database specifically created for this study and subsequently produced as recombinant antigens. The ability of recombinant antigens to discriminate patients with FLD from controls was assessed by ELISA performed with sera from FLD patients (n = 41) and controls (n = 43) recruited from five university hospital pneumology departments of France and Switzerland. Forty-one FLD-associated immunoreactive proteins from L. corymbifera were identified. Six of them were produced as recombinant antigens. With a sensitivity and specificity of 81.4 and 77.3% respectively, dihydrolipoyl dehydrogenase was the most effective antigen for discriminating FLD patients from HEC. ELISA performed with the putative proteasome subunit alpha type as an antigen was especially specific (88.6%) and could thus be used for FLD confirmation. The production of recombinant antigens from L. corymbifera represents an additional step towards the development of a standardized ELISA kit for FLD diagnosis.
ISSN: 1932-6203

hal-01368315
https://hal.archives-ouvertes.fr/hal-01368315
DOI : 10.1371/journal.pone.0160888
PUBMED : 27490813
PUBMEDCENTRAL : PMC4973876

Éditeur(s) : Londres : Sampson Law, Marston, Searle & Rivington
Siècle(s) traité(s) : 19
Provenance : Archives départementales de Guadeloupe
Permalien : http://www.manioc.org/patrimon/ADG18114
ADG18114

Éditeur(s) : Paris : Imprimerie nationale
Siècle(s) traité(s) : 18
Droits : Domaine public
Provenance : Communauté d'agglomération de La Rochelle. Médiathèque Michel-Crépeau
Permalien : http://www.manioc.org/patrimon/MMC16066-6
MMC16066-6

Éditeur(s) : Université des Antilles Études caribéennes
Résumé : Avec 130 espèces identifiées, l’Amazonie est la région du monde qui abrite le plus grand nombre d’espèces d’abeilles sans dard. Cette diversité reste assez peu exploitée par les communautés locales, même si certaines pratiques, comme la recherche de nids ou l’extraction de miel sauvage, sont sans doute très anciennes. La diversification des usages du miel et l’utilisation récente du pollen à des fins médicinales, pourraient les modifier durablement, et les essaims devenir l’objet de toutes les attentions.Dans la Réserve d’Amanã, les dix-huit agriculteurs, que nous avons interrogés et parfois filmés lors de mises en situation, ont conscience de cette évolution et la plupart s’en réjouissent. En tant qu’éleveurs d’abeilles, ils savent aujourd’hui que pour récolter du miel, ils ne sont plus contraints de chercher des colonies sauvages, de les déloger pour finalement les détruire. Mais on aurait tort de croire que ces pratiques anciennes constituent, dans le cas étudié, une menace pour l’entomofaune ; d’une part parce que la pression sur les nids reste très limitée, d’autre part parce que les extractions ne concernent qu’un nombre réduit d’espèces. C’est pourquoi il est indispensable de mieux décrire ces techniques et de les valoriser comme des savoir-faire ancestraux.
With 130 identified species, the Amazon is one region of the world which shelters the largest number of species of bees without a sting. This diversity is rarely exploited by the local communities even if certain practices like the search of honeycombs or the extraction of wild honey are very ancient. The diversification of the uses of honey and the recent use of the pollen for medicinal purposes could modify these sustainable practices and the bee swarms could become a major center of attention.In the Amanã Reserve, the eighteen farmers that were questioned and sometimes filmed while they were in work situations, are conscious of this revolution and most of them rare pleased with this new trend. As working beekeepers, they know that to collect some honey, they are no longer compelled to look for wild colonies, to dislodge them from their natural habitat, which would finally destroy them. Correspondingly it would be wrong to believe that these ancient practices, extraction of wild honey, represent in the studied case, a threat for the entomofauna. On one hand, the importance on honeycombs remains very limited, also on the other hand, the extractions concern just a few number of bee species. That is why it is essential to better specify these techniques and to value them as an ancestral knowledge.
Brésil

Droits : info:eu-repo/semantics/openAccess
urn:doi:10.4000/etudescaribeennes.10279
http://journals.openedition.org/etudescaribeennes/10279

Éditeur(s) :  :
Siècle(s) traité(s) : 19
Droits : Domaine public
Provenance : Collectivité territoriale de Martinique. Bibliothèque Schoelcher
Permalien : http://www.manioc.org/patrimon/SCH13244
SCH13244