Éditeur(s) : HAL CCSD Wiley
Résumé : International audience
Within the framework of the international field campaign COPS (Convective and Orographically induced Precipitation Study), a large suite of state-of-the-art meteorological instrumentation was operated, partially combined for the first time. This includes networks of in situ and remote-sensing systems such as the Global Positioning System as well as a synergy of multi-wavelength passive and active remote-sensing instruments such as advanced radar and lidar systems. The COPS field phase was performed from 01 June to 31 August 2007 in a low-mountain area in southwestern Germany/eastern France covering the Vosges mountains, the Rhine valley and the Black Forest mountains. The collected dataset covers the entire evolution of convective precipitation events in complex terrain from their initiation, to their development and mature phase until their decay. Eighteen Intensive Observations Periods with 37 operation days and eight additional Special Observations Periods were performed, providing a comprehensive dataset covering different forcing conditions. In this article, an overview of the COPS scientific strategy, the field phase, and its first accomplishments is given. Highlights of the campaign are illustrated with several measurement examples. It is demonstrated that COPS research provides new insight into key processes leading to convection initiation and to the modification of precipitation by orography, in the improvement of quantitative precipitation forecasting by the assimilation of new observations, and in the performance of ensembles of convection-permitting models in complex terrain.
ISSN: 0035-9009

hal-00565513
https://hal.archives-ouvertes.fr/hal-00565513
DOI : 10.1002/qj.752

Éditeur(s) : HAL CCSD Wiley
Résumé : International audience
An intensive water vapour intercomparison effort, involving airborne and ground-based water vapour lidar systems, was carried out in the framework of the COPS experiment. The main objective of this paper is to provide accurate error estimates for these systems. Comparisons between the ground-based Raman lidar BASIL and the airborne CNRS DIAL (Differential Absorption Lidar) indicate a mean relative bias between the two sensors, calculated with respect to the mean value of −2.13% (−0.034 g kg−1) in the altitude region 0.5-3.5 km, while comparisons between BASIL and the airborne DLR DIAL lead to a mean relative bias of 1.87% (0.018 g kg−1) in this same altitude region. Comparisons between the ground-based UHOH DIAL and the CNRS DIAL indicate a bias of −3.2% (−0.37 × 1022 m−3) in the altitude range 1.5-4.5 km, while comparisons between the UHOH DIAL and the DLR DIAL indicate a bias of 0.83% (0.06 × 1022 m−3) in this same altitude range. Based on the available comparisons between the ground-based Raman lidar BERTHA and the CNRS DIAL, the mean relative bias is found to be −4.37% (−0.123 g kg−1) in the altitude region 0.5-4.5 km. Comparisons between the ground-based IGN Raman lidar and the CNRS DIAL indicate a bias of 3.18% (0.55 g kg−1) in the altitude range from 0.5 to 4.5 km, while comparisons between the CNRS DIAL and DLR DIAL result in a mean relative bias of 3.93% (1.1 × 1022 m−3) in the altitude interval 0.5-4.0 km. Based on the available statistics of comparisons, benefiting from the fact that the CNRS DIAL was able to be compared with all other lidar systems, and putting equal weight on the data reliability of each instrument, overall relative values for BASIL, BERTHA, IGN Raman lidar, UHOH DIAL, DLR DIAL, and CNRS DIAL, with respect to the mean value, are found to be −0.38, −2.60, 4.90, −1.43, −2.23 and 1.72%, respectively.
ISSN: 0035-9009

hal-00572576
https://hal.archives-ouvertes.fr/hal-00572576
DOI : 10.1002/qj.697

Éditeur(s) : HAL CCSD Oxford University Press
Résumé : International audience
It was recently reported that the sizes of many mRNAs change when budding yeast cells exit mitosis and enter the meiotic differentiation pathway. These differences were attributed to length variations of their untranslated regions. The function of UTRs in protein translation is well established. However, the mechanism controlling the expression of distinct transcript isoforms during mitotic growth and meiotic development is unknown. In this study, we order developmentally regulated transcript isoforms according to their expression at specific stages during meiosis and gametogenesis, as compared to vegetative growth and starvation. We employ regulatory motif prediction, in vivo protein-DNA binding assays, genetic analyses and monitoring of epigenetic amino acid modification patterns to identify a novel role for Rpd3 and Ume6, two components of a histone deacetylase complex already known to repress early meiosis-specific genes in dividing cells, in mitotic repression of meiosis-specific transcript isoforms. Our findings classify developmental stage-specific early, middle and late meiotic transcript isoforms, and they point to a novel HDAC-dependent control mechanism for flexible transcript architecture during cell growth and differentiation. Since Rpd3 is highly conserved and ubiquitously expressed in many tissues, our results are likely relevant for development and disease in higher eukaryotes.
ISSN: 0305-1048

Droits : http://creativecommons.org/licenses/by/
hal-01122128
https://hal-univ-rennes1.archives-ouvertes.fr/hal-01122128
https://hal-univ-rennes1.archives-ouvertes.fr/hal-01122128/document
https://hal-univ-rennes1.archives-ouvertes.fr/hal-01122128/file/The%20conserved%20histone%20deacetylase.pdf
DOI : 10.1093/nar/gku1185

Éditeur(s) : HAL CCSD Wiley
Résumé : International audience
In the mid-afternoon of 15 July 2007, during the Convective and Orographically induced Precipitation Study (COPS), in a very warm and dry environment, an isolated, short-lived, deep convective system developed over the southern Black Forest. Most of the high-resolution, convection-permitting models involved in COPS were unable to capture this event whereas the Meso-NH forecast was quite skilful. To assess the Meso-NH performance further, the model results were carefully checked against the various and numerous COPS observations. In full agreement with clear-air radar observations, model results underlined the triggering role of a low-level convergence line that developed in the lee of the Feldberg. The main departure from the observations was found to be in the low-level moisture fields, which appeared significantly moister in the model than in the observations and also slightly moister than in the other models. Sensitivity studies showed that this departure from the observations was strongly controlled by the initial surface moisture conditions. When the surface moisture was reduced by 20% or replaced by the value derived from a different analysis, the evolution of the planetary boundary layer was more accurately represented while the storm evolution was still correctly captured. These results demonstrate that the quality of the initial forecast cannot be ascribed to the moist bias of the model. It could therefore be hypothesized that the key parameters for a satisfactory forecast of this event lie more in the ability of the model to reproduce the dynamical forcing accurately than in the characteristics of the air-mass instability.
ISSN: 0035-9009

hal-00554400
https://hal.archives-ouvertes.fr/hal-00554400
DOI : 10.1002/qj.710

Éditeur(s) : HAL CCSD Wiley
Résumé : International audience
The Convective and Orographically-driven Precipitation Study (COPS) carried out in summer 2007 over northeastern France and southwestern Germany provided a fairly comprehensive description of the low-troposphere water-vapour field, thanks in particular to the deployment of two airborne differential absorption lidar systems. These lidar observations were assimilated using the 3D-Var assimilation system of the Application of Research to Operations at MEsoscale (AROME) numerical weather prediction mesoscale model. The assimilation was carried out for the period 4 July-3 August by running a three-hour forward intermittent assimilation cycle. First, the impact of the lidar observations was assessed by comparing the analyses with a set of more than 200 independent soundings. The lidar observations were found to have a positive impact on the analyses by reducing the dry bias in the first 500 m above ground level and by diminishing the root-mean-square error by roughly 15% in the first km. Then the impact of the lidar observations was assessed by comparing the precipitation forecasts (obtained with and without the lidar observations for the period 15 July-2 August) with the gridded precipitation observations provided by the Vienna Enhanced Resolution Analysis. In general, the impact was found to be positive but not significant for the 24 h precipitation and positive and significant for the 6 h precipitation, with an improvement lasting up to 24 h. Some selected case studies show that the improvement was obtained through a better depiction of convection initiation or through a more accurate positioning of the precipitation systems
ISSN: 0035-9009

hal-00659497
https://hal.archives-ouvertes.fr/hal-00659497
DOI : 10.1002/qj.1864

Éditeur(s) : HAL CCSD springer Link
Résumé : International audience
Experimental Astronomy

hal-01094521
https://hal.archives-ouvertes.fr/hal-01094521
BIBCODE : 2014ExA...tmp...41R
DOI : 10.1007/s10686-014-9383-4
ARXIV : 1310.0696

Éditeur(s) : HAL CCSD
Résumé : International audience
BACKGROUND: The effects of pressure-controlled (PC) ventilation on the ventilatory and haemodynamic parameters during laparoscopy procedures had not been carefully assessed. This prospective cross-over study was undertaken to compare how volume-controlled (VC) and PC modes could affect pulmonary mechanics, gas exchange, and cardiac function in patients undergoing laparoscopy. METHODS: Twenty-one patients undergoing laparoscopic urological procedures had their lungs ventilated at the beginning with VC ventilation. PC ventilation was instituted at the end of the VC sequence. Ventilator settings were adjusted to keep tidal volume, respiratory rate, and Fi(o(2)) constant in every mode. A complete set of ventilatory, haemodynamic, and gas exchange parameters was obtained under VC after 40 min of pneumoperitoneum and 20 min after switching for PC. Transoesophageal echocardiography was performed in order to evaluate systolic and diastolic function of the heart. RESULTS: When VC was switched to PC, peak airway pressure decreased [mean (sd) 32 (6) vs 27 (6) cm H(2)O; P < 0.0001], peak inspiratory flow increased [17 (3) vs 48 (8) litre min(-1); P < 0.0001), and dynamic compliance improved [+15 (8)%]. No difference was noted for static airway pressure, static compliance, and arterial oxygenation. No significant change could be demonstrated in the systolic [left ventricular end-systolic wall stress 66 (16) vs 63 (14) x 10(3) dyn cm(-2) m(-2)] or diastolic function [early diastolic velocity 10.3 (2.5) vs 10.5 (2.7) cm s(-1)]. CONCLUSIONS: In this study, no short-term beneficial effect of PC ventilation could be demonstrated over conventional VC ventilation in patients with pneumoperitoneum.
BJA

hal-00689758
https://hal.archives-ouvertes.fr/hal-00689758
DOI : 10.1093/bja/aem166
PUBMED : 17626027

Éditeur(s) : HAL CCSD Springer Verlag
Résumé : International audience
Tuftelin (TUFT1) is an acidic, phosphorylated glycoprotein, initially discovered in developing enamel matrix. TUFT1 is expressed in many mineralized and non-mineralized tissues. We performed an evolutionary analysis of 82 mammalian TUFT1 sequences to identify residues and motifs that were conserved during 220 million years (Ma) of evolution. We showed that 168 residues (out of the 390 residues composing the human TUFT1 sequence) are under purifying selection. Our analyses identified several, new, putatively functional domains and confirmed previously described functional domains, such as the TIP39 interaction domain, which correlates with nuclear localization of the TUFT1 protein, that was demonstrated in several tissues. We also identified several sites under positive selection, which could indicate evolutionary changes possibly related to the functional diversification of TUFT1 during evolution in some lineages. We discovered that TUFT1 and MYZAP (myocardial zonula adherens protein) share a common ancestor that was duplicated circa 500 million years ago. Taken together, these findings expand our knowledge of TUFT1 evolution and provide new information that will be useful for further investigation of TUFT1 functions.
ISSN: 0022-2844

hal-01516886
http://hal.upmc.fr/hal-01516886
http://hal.upmc.fr/hal-01516886/document
http://hal.upmc.fr/hal-01516886/file/Delgado_2017_Evolutionary.pdf
DOI : 10.1007/s00239-017-9789-5

Éditeur(s) : HAL CCSD
Résumé : International audience
In two swordtail species of the genus Xiphophorus, the onset of puberty has been shown to be modulated at the P locus by sequence polymorphism and gene copy-number variation affecting the type 4 melanocortin hormone receptor Mc4r. The system works through the interaction of two allelic types, one encoding wild type and the other dominant-negative receptors. We have analyzed the structure and evolution of the P locus in the platyfish Xiphophorus maculatus, where as many as nine alleles of P determining the onset of sexual maturity in males and females, fecundity in females, and adult size in males are located on both the X and Y chromosomes in a region linked to the master sex-determining locus. In this species, mc4r has been amplified to up to 10 copies on both the X and Y chromosomes through recent large serial duplications. Subsequently, mc4r paralogues have diverged considerably into many different subtypes. Certain copies have acquired new untranslated regions through genomic rearrangements, and transposable element insertions and other mutations have accumulated in promoter regions, possibly explaining observed deviations from the classical mc4r transcriptional pattern. In the mc4r-coding sequence, in-frame insertions and deletions as well as nonsense and missense mutations have generated a high diversity of Mc4r-predicted proteins. Most of these variants are expressed in embryos, adults, and/or tumors. Functional receptor characterization demonstrated major divergence in pharmacological behavior for Mc4r receptors encoded by different copies of platyfish mc4r, with differences in constitutive activity as well as binding and stimulation by hormones. The high degree of allelic and copy-number variation observed between individuals can explain the high level of polymorphism for sexual maturation, fecundity, and body size in the platyfish: multiple combinations of Mc4r variants with different biochemical properties might interact to modulate the melanocortin signaling that regulates the hypothalamus-pituitary-gonadal axis.
GENETICS

hal-01544912
https://hal.archives-ouvertes.fr/hal-01544912
DOI : 10.1534/genetics.113.155952

Éditeur(s) : HAL CCSD Wiley-VCH Verlag
Résumé : International audience
Deciphering the assembly history of the Milky Way is a formidable task, which becomes possible only if one can produce high-resolution chrono-chemo-kinematical maps of the Galaxy. Data from large-scale astrometric and spectroscopic surveys will soon provide us with a well-defined view of the current chemo-kinematical structure of the Milky Way, but will only enable a blurred view on the temporal sequence that led to the present-day Galaxy. As demonstrated by the (ongoing) exploitation of data from the pioneering photometric missions CoRoT, Kepler, and K2, asteroseismology provides the way forward: solar-like oscillating giants are excellent evolutionary clocks thanks to the availability of seismic constraints on their mass and to the tight age-initial-mass relation they adhere to. In this paper we identify five key outstanding questions relating to the formation and evolution of the Milky Way that will need precise and accurate ages for large samples of stars to be addressed, and we identify the requirements in terms of number of targets and the precision on the stellar properties that are needed to tackle such questions. By quantifying the asteroseismic yields expected from PLATO for red-giant stars, we demonstrate that these requirements are within the capabilities of the current instrument design, provided that observations are sufficiently long to identify the evolutionary state and allow robust and precise determination of acoustic-mode frequencies. This will allow us to harvest data of sufficient quality to reach a 10% precision in age. This is a fundamental pre-requisite to then reach the more ambitious goal of a similar level of accuracy, which will only be possible if we have to hand a careful appraisal of systematic uncertainties on age deriving from our limited understanding of stellar physics, a goal which conveniently falls within the main aims of PLATO's core science.
ISSN: 0004-6337

hal-01541393
https://hal.archives-ouvertes.fr/hal-01541393
ARXIV : 1706.3778
BIBCODE : 2017AN....338..644M
DOI : 10.1002/asna.201713385

Éditeur(s) : HAL CCSD Météo et Climat
Résumé : International audience
The Convective and Orographically- induced Precipitation Study (COPS) is a coordinated international project, comprised of an observational field campaign and a research programme aiming to advance the quality of fore- casts of orographically-induced pre- cipitation by four-dimensional obser- vations and modelling of its life cycle. The COPS field campaign took place during June-July-August 2007 over eastern France and south-western Germany. Its main objective was to provide an unprecedented comprehen- sive set of in situ and remotely-sensed meteorological observations of the entire depth of the troposphere. After a short overview of the project, the paper focuses on the French contribution to the COPS field phase, describes the experimental setup and highlights some key observations.
L’étude des précipitations convectives induites par l’orographie (Cops selon l’acronyme anglais) est un projet inter- national coordonné qui comprend une campagne d’observations de terrain et un programme scientifique et qui vise à améliorer la qualité des prévisions des précipitations orographiques convectives à la fois par l’observation quadridimensionnelle et la modélisa- tion de leur cycle de vie. La campagne de terrain s’est déroulée en juin-juillet- août 2007 sur l’est de la France et le sud-ouest de l’Allemagne. Son objectif majeur était de fournir un jeu d’obser- vations météorologiques, in situ et à distance, aussi complet que possible et sur toute la profondeur de la colonne troposphérique.Après une brève revue d’ensemble du projet, l’article se concentre sur la contribution française à la campagne Cops, décrit le dispositif expérimental mis en œuvre et met en valeur quelques observations clés.
ISSN: 0026-1181

insu-01504075
https://hal-insu.archives-ouvertes.fr/insu-01504075
https://hal-insu.archives-ouvertes.fr/insu-01504075/document
https://hal-insu.archives-ouvertes.fr/insu-01504075/file/meteo_2009_64_32.pdf
DOI : 10.4267/2042/23632

Éditeur(s) : HAL CCSD Springer Verlag
Résumé : The diversity and functional identity of organisms are known to be relevant to the maintenance of ecosystem processes but can be variable in different environments. Particularly, it is uncertain whether ecosystem processes are driven by complementary effects or by dominant groups of species. We investigated how community structure (i.e., the diversity and relative abundance of biological entities) explains the community-level contribution of Neotropical ant communities to different ecosystem processes in different environments. Ants were attracted with food resources representing six ant-mediated ecosystem processes in four environments: ground and vegetation strata in cropland and forest habitats. The exploitation frequencies of the baits were used to calculate the taxonomic and trophic structures of ant communities and their contribution to ecosystem processes considered individually or in combination (i.e., multifunctionality). We then investigated whether community structure variables could predict ecosystem processes and whether such relationships were affected by the environment. We found that forests presented a greater biodiversity and trophic complementarity and lower dominance than croplands, but this did not affect ecosystem processes. In contrast, trophic complementarity was greater on the ground than on vegetation and was followed by greater resource exploitation levels. Although ant participation in ecosystem processes can be predicted by means of trophic-based indices, we found that variations in community structure and performance in ecosystem processes were best explained by environment. We conclude that determining the extent to which the dominance and complementarity of communities affect ecosystem processes in different environments requires a better understanding of resource availability to different species.
ISSN: 0029-8549

hal-01606497
https://hal.archives-ouvertes.fr/hal-01606497
DOI : 10.1007/s00442-016-3741-z
PRODINRA : 407457

Éditeur(s) : HAL CCSD Oxford University Press (OUP)
Résumé : International audience
Deciphering the evolutionary processes driving nucleotide variation in multiallelic genes is limited by the number of genetic systems in which such genes occur. The complementary sex determiner (csd) gene in the honey bee Apis mellifera is an informative example for studying allelic diversity and the underlying evolutionary forces in a well-described model of balancing selection. Acting as the primary signal of sex determination, diploid individuals heterozygous for csd develop into females, whereas csd homozygotes are diploid males that have zero fitness. Examining 77 of the functional heterozygous csd allele pairs, we established a combinatorical criteria that provide insights into the minimum number of amino acid differences among those pairs. Given a data set of 244 csd sequences, we show that the total number of csd alleles found in A. mellifera ranges from 53 (locally) to 87 (worldwide), which is much higher than was previously reported (20). Using a coupon-collector model, we extrapolate the presence of in total 116-145 csd alleles worldwide. The hypervariable region (HVR) is of particular importance in determining csd allele specificity, and we provide for this region evidence of high evolutionary rate for length differences exceeding those of microsatellites. The proportion of amino acids driven by positive selection and the rate of nonsynonymous substitutions in the HVR-flanking regions reach values close to 1 but differ with respect to the HVR length. Using a model of csd coalescence, we identified the high originating rate of csd specificities as a major evolutionary force, leading to an origin of a novel csd allele every 400,000 years. The csd polymorphism frequencies in natural populations indicate an excess of new mutations, whereas signs of ancestral transspecies polymorphism can still be detected. This study provides a comprehensive view of the enormous diversity and the evolutionary forces shaping a multiallelic gene.
ISSN: 0737-4038

hal-01544758
https://hal.archives-ouvertes.fr/hal-01544758
DOI : 10.1093/molbev/mst207