Éditeur(s) : HAL CCSD Oxford University Press (OUP)
Résumé : International audience
Are bisphenol A (BPA) and BPA analogs (BPA-A) safe for male human reproductive function? The endocrine function of human testes explants [assessed by measuring testosterone and insulin-like factor 3 (INSL3)] was impacted by exposure of the human adult testis explants to BPA/BPA-A. The few epidemiologic studies performed suggest that bisphenols have potential endocrine disruptive properties, but they did not identify clear and direct patterns of endocrine disruption. Adult human testis explants in culture were exposed to BPA and the analogs bisphenol F (BPF), bisphenol S (BPS), bisphenol E (BPE), bisphenol B (BPB) and bisphenol A diglycidyl ether (BADGE) at 10(-9)-10(-5) M for 24 or 48 h. Human adult testes were obtained from prostate cancer patients who had no hormone therapy, or from multiorgan donors. After ex vivo exposure to the investigated bisphenols, the measured outcomes were related to histopathology (gross morphology and germ cell viability determined by anti-caspase three immunohistochemistry), and the levels of testosterone, INSL3 and inhibin B were measured using immunoassays. The levels of mRNA encoding key enzymes of bisphenol biotransformation were investigated by quantitative PCR: UGT2B15 UDP (glucuronosyltransferase two family, polypeptide B15), GUSB (glucuronidase beta), SULT1A1 and 3 (sulfotransferase family 1 A member 1 and 3) and STS (steroid sulfatase). A significant dose-dependent inhibition was found between testosterone levels measured in the culture medium and concentrations of BPA (P = 0.00778 at 24 h and P = 0.0291 at 48 h), BPE (P = 0.039) and BPF (P = 0.00663). The observed BPA and BPA-A-induced inhibition of testosterone production varied according to duration of exposure and BPA/BPA-A concentrations. BPA (10(-9) M; P < 0.05), BPB (10(-9) M; P < 0.05), BPS (10(-9) and 10(-8) M; P < 0.05) and BADGE (10(-5) M; P < 0.05) increased Leydig cell INSL3 production. By contrast, BPE dose dependently inhibited INSL3 (P = 0.0372). Conversely, Sertoli cell function (inhibin B) and germ cell viability were not significantly affected by either bisphenols. N/A. Environmental compounds cannot be deliberately administered to men, justifying the use of an ex vivo approach. A relatively low number of testes samples were available for analysis (n = 3, except for testosterone secretion with n = 5). The active concentrations of BPA and BPA-A used in the study were higher than those found in human biological fluids. Under our experimental conditions, direct exposure to BPA or BPA-A can result in endocrine disturbance in the adult human testis.
ISSN: 0268-1161

hal-01578582
https://hal-univ-rennes1.archives-ouvertes.fr/hal-01578582
https://hal-univ-rennes1.archives-ouvertes.fr/hal-01578582/document
https://hal-univ-rennes1.archives-ouvertes.fr/hal-01578582/file/Parallel%20assessment%20of%20the%20effects%20of%20bisphenol%20A_accepted.pdf
DOI : 10.1093/humrep/dex093

Éditeur(s) : HAL CCSD Public Library of Science
Résumé : International audience
Few studies have been undertaken to assess the possible effects of bisphenol A (BPA) on the reproductive hormone balance in animals or humans with often contradictory results. We investigated possible direct endocrine disruption by BPA of the fetal testes of 2 rat strains (14.5-17.5 days post-coitum) and humans (8-12 gestational weeks) and under different culture conditions. BPA concentrations of 10-8M and 10-5M for 72h reduced testosterone production by the Sprague-Dawley fetal rat testes, while only 10-5M suppressed it in the Wistar strain. The suppressive effects at 10-5M were seen as early as 24h and 48h in both strains. BPA at 10-7-10-5M for 72h suppressed the levels of fetal rat Leydig cell insulin-like factor 3 (INSL3). BPA exposure at 10-8M, 10-7M, and 10-5M for 72h inhibited testosterone production in fetal human testes. For the lowest doses, the effects observed occurred only when no gonadotrophin was added to the culture media and were associated with a poorly preserved testicular morphology. We concluded that (i) BPA can display anti-androgenic effects both in rat and human fetal testes; (ii) it is essential to ascertain that the divergent effects of endocrine disruptors between species in vitro do not result from the culture conditions used, and/or the rodent strain selected; (iii) the optimization of each in vitro assay for a given species should be a major objective rather than the search of an hypothetical trans-species consensual model-system, as the organization of the testis is intrinsically different between mammalian species; (iv) due to the uncertainty existing on the internal exposure of the human fetal testis to BPA, and the insufficient number of epidemiological studies on the endocrine disruptive effects of BPA, caution should be taken in the extrapolation of our present results to the human reproductive health after fetal exposure to BPA.
ISSN: 1932-6203

hal-01121759
https://hal-univ-rennes1.archives-ouvertes.fr/hal-01121759
PUBMED : 25706302
DOI : 10.1371/journal.pone.0117226

Éditeur(s) : HAL CCSD
Résumé : Benzophenone derivatives (BPs) are used as UV filters in many different products. These lipophilic compounds were recently described as ubiquitous contaminant of different compartiments of aquatic systems, including fish. In this study, we evaluated estrogenic activity of ten BPs in a model fish species, the zebrafish (Danio rerio), by combining newly developed in vitro and in vivo models. In vitro, three specific zebrafish reporter gene models expressing zebrafish estrogen receptors (ZfERs) in zebrafish hepatic cell line (ZFL) were used.Estrogenic activity of BPs was quantify in vivo by using a newly developed transgenic zebrafish line that expresses green fluorescent protein (GFP) under the control of the cyp19a1b promoter. The cyp19a1b gene codes for the brain aromatase B that is under the control of estrogens. Vitellogenin (Vtg), a marker of estrogenic compound, was used to assess the potential estrogenic effect of certain BPs in male fish. Five of 10 BPs induced an estrogenic activity on the three models: BP2, THB, BP1, 4BP, while BP and 234 BP were non active. We noted diffrence in BPs ranking depending on the zfER subtypes suggesting ?/? receptor selectivity by certain BPs. By quantifying GFP expression in live zebrafish, we confirm the estrogenic activity of some BP, i.e., BP1, THB, 4BP and 44'BP, as shown by their ability to induce GFP in a concentration-dependent manner. However, the ranking of BPs as a function of their estrogenic activity assay is different between models. For instance, 4BP was the most estrogenic compound in vivo but not in vitro. Differences were also noticed regarding the estrogenic activity of BP2. In vitro, BP2 has strong activity but had no effect on GFP in cyp19a1b-GFP embryo. However, BP2 induced strongly Vtg in male. The lack of estrogenic activity of BP2 in embryo may be related to difference of biodisponibility and/or metabolisation of this compound between embryo and adult fish. Our approach allows to characterize the estrogenic potency of emerging pollutants, using innovative in vitro and in vivo assays within a unique model fish species. The differences observed between assays deserve further studies to characterize the behavior of compounds in the different biological models in term of biodisponibility and metabolism.
1. European Conference on the Replacement, Reduction and Refinement of Animal Experiments in Ecotoxicology
Dübendorf, Switzerland

ineris-00971019
https://hal-ineris.archives-ouvertes.fr/ineris-00971019
INERIS : EN-2012-243

Éditeur(s) : HAL CCSD Springer Verlag
Résumé : International audience
The Nile delta sedimentation constitutes a continuous high resolution (1.6 mm/year) record of Ethiopian African monsoon regime intensity. Multiproxy analyses performed on core MS27PT recovered in hemipelagic Nile sediment margin (<90 km outward of the Rosetta mouth of the Nile) allow the quantification of the Saharan aeolian dust and the Blue/White Nile River suspended matter frequency fluctuations during the last 21 cal. ka BP. The radiogenic Sr and Nd isotopes, clay mineralogy, bulk elemental composition and palynological analyses reveal large changes in source components, oscillating between a dominant aeolian Saharan contribution during the LGM and the Late Holocene (~4 to 2 cal. ka BP), a dominant Blue/Atbara Nile River contribution during the early Holocene (15 to 8.4 cal. ka BP) and a probable White Nile River contribution during the Middle Holocene (8.4 to 4 cal. ka BP). The following main features are highlighted: 1. The rapid shift from the LGM arid conditions to the African Humid Period (AHP) started at about 15 cal. ka BP. AHP extends until 8.4 cal. ka BP, and we suggest that the Ethiopian African Monsoon maximum between 12 and 8 cal. ka BP is responsible for a larger Blue/Atbara Nile sediment load and freshwater input into the Eastern Mediterranean Sea. 2. The transition between the AHP and the arid Late Holocene is gradual and occurs in two main phases between 8.4 and 6.5 cal. ka BP and 6.5 to 3.2 cal. ka BP. We suggest that the main rain belt shifted southward from 8.4 to ~4 cal. ka BP and was responsible for progressively reduced sediment load and freshwater input into the eastern Mediterranean Sea. 3. The aridification along the Nile catchments occurred from ~4 to 2 cal. ka BP. A dry period, which culminates at 3.2 cal. ka BP, and seems to coincide with a re-establishment of increased oceanic primary productivity in the western Mediterranean Sea. We postulate that the decrease in thermo-haline water Mediterranean circulation could be part of a response to huge volumes of fresh-water delivered principally by the Nile River from 12 to 8.4 cal. ka BP in the eastern Mediterranean. We propose that the large hydrological change in Ethiopian latitude could be a trigger for the 8.2 ka cooling event recorded in high latitude. Revel R., Colin C., Bernasconi S., Combourieu-Nebout N., Ducassou E., Grousset F.E., Rolland Y., Migeon S., Brunet P., Zhaa Y., Bosch D., Mascle J.,. "21,000 years of Ethiopian African moonsoon variability recorded in sediments of the western Nile deep sea fan", Regional Environmental Change,
ISSN: 1436-3798

hal-00836300
https://hal.archives-ouvertes.fr/hal-00836300

Éditeur(s) : Copernicus Gesellschaft Mbh
Résumé : From 10 to 8 ka BP (thousand years before present), paleoclimate records show an atmospheric and oceanic cooling in the high latitudes of the Southern Hemisphere. During this interval, temperatures estimated from proxy data decrease by 0.8 degrees C over Antarctica and 1.2 degrees C over the Southern Ocean. In order to study the causes of this cooling, simulations covering the early Holocene have been performed with the climate model of intermediate complexity LOVECLIM constrained to follow the signal recorded in climate proxies using a data assimilation method based on a particle filtering approach. The selected proxies represent oceanic and atmospheric surface temperature in the Southern Hemisphere derived from terrestrial, marine and glaciological records. Two mechanisms previously suggested to explain the 10-8 ka BP cooling pattern are investigated using the data assimilation approach in our model. The first hypothesis is a change in atmospheric circulation, and the second one is a cooling of the sea surface temperature in the Southern Ocean, driven in our experimental setup by the impact of an increased West Antarctic melting rate on ocean circulation. For the atmosphere hypothesis, the climate state obtained by data assimilation produces a modification of the meridional atmospheric circulation leading to a 0.5 degrees C Antarctic cooling from 10 to 8 ka BP compared to the simulation without data assimilation, without congruent cooling of the atmospheric and sea surface temperature in the Southern Ocean. For the ocean hypothesis, the increased West Antarctic freshwater flux constrainted by data assimilation (+100 mSv from 10 to 8 ka BP) leads to an oceanic cooling of 0.7 degrees C and a strengthening of Southern Hemisphere westerlies (+6 %). Thus, according to our experiments, the observed cooling in Antarctic and the Southern Ocean proxy records can only be reconciled with the reconstructions by the combination of a modified atmospheric circulation and an enhanced freshwater flux.
Climate Of The Past (1814-9324) (Copernicus Gesellschaft Mbh), 2013 , Vol. 9 , N. 2 , P. 887-901

Droits : Author(s) 2013. CC Attribution 3.0 License.
http://archimer.ifremer.fr/doc/00181/29203/27584.pdf
DOI:10.5194/cp-9-887-2013
http://archimer.ifremer.fr/doc/00181/29203/

Éditeur(s) : HAL CCSD Public Library of Science
Résumé : International audience
Patients with sickle cell anemia (SCA) have usually lower diastolic, systolic and mean blood pressure (BP) than the general population. However, BP values ≥120/70 mmHg considerably increase the risk for acute and chronic complications in SCA. The aim of this study was to identify biological factors associated with relative hypertension in adults with SCA. We compared the hematological, lipid and hemolytic profiles, as well as blood viscosity, between SCA patients with normal BP (<120/70 mmHg, n = 54) and those with relative hypertension (BP≥120/70 mmHg, n = 43). Our results demonstrated that male gender (OR: 3.49; 95%CI 1.20 to 10.16, p<0.05), triglycerides (OR: 9.19; 95% CI 2.29 to 36.95, p<0.01), blood viscosity (OR: 1.35; 95% CI 1.01 to 1.81, p<0.05) and body mass index (OR: 1.37; 95% CI 1.14 to 1.64, p<0.01) were independent risks factors for relative hypertension in SCA. No association was found between the BP status and the positive history of painful vaso-occlusive crisis or acute chest syndrome. An association between triglycerides level and the occurrence of these two major acute complications was detected. Our study suggests that male gender, increased triglycerides level, BMI and blood viscosity could increase the risk for developing relative hypertension in SCA. In addition, our results support a role of moderately elevated triglycerides in the pathophysiology of vaso-occlusive events.
ISSN: 1932-6203

inserm-00838991
http://www.hal.inserm.fr/inserm-00838991
http://www.hal.inserm.fr/inserm-00838991/document
http://www.hal.inserm.fr/inserm-00838991/file/Lamarre_et_al._2013_PloS_ONE_.pdf
DOI : 10.1371/journal.pone.0066004

Éditeur(s) : Wiley-blackwell Publishing, Inc
Résumé : Vibrio splendidus, strain LGP32, is an oyster pathogen associated with the summer mortalities affecting the production of Crassostrea gigas oysters worldwide. Vibrio splendidus LGP32 was shown to resist to up to 10 mu M Cg-Def defensin and Cg-BPI bactericidal permeability increasing protein, two antimicrobial peptides/proteins (AMPs) involved in C. gigas immunity. The resistance to both oyster Cg-Def and Cg-BPI and standard AMPs (polymyxin B, protegrin, human BPI) was dependent on the ompU gene. Indeed, upon ompU inactivation, minimal bactericidal concentrations decreased by up to fourfold. AMP resistance was restored upon ectopic expression of ompU. The susceptibility of bacterial membranes to AMP-induced damages was independent of the ompU-mediated AMP resistance. Besides its role in AMP resistance, ompU proved to be essential for the adherence of V. splendidus LGP32 to fibronectin. Interestingly, in vivo, ompU was identified as a major determinant of V. spiendidus pathogenicity in oyster experimental infections. Indeed, the V. splendidus-induced oyster mortalities dropped from 56% to 11% upon ompU mutation (Kaplan Meier survival curves, P < 0.01). Moreover, in co-infection assays, the ompU mutant was out competed by the wild-type strain with competitive indexes in the range of 0.1-0.2. From this study, ompU is required for virulence of V. splendidus. Contributing to AMP resistance, conferring adhesive properties to V. splendidus, and being essential for in vivo fitness, the OmpU porin appears as an essential effector of the C. gigas/V. splendidus interaction.
Environmental Microbiology (1462-2912) (Wiley-blackwell Publishing, Inc), 2010-04 , Vol. 12 , N. 4 , P. 951-963

Droits : 2010 Society for Applied Microbiology and Blackwell Publishing Ltd
http://archimer.ifremer.fr/doc/00003/11421/8044.pdf
DOI:10.1111/j.1462-2920.2009.02138.x
http://archimer.ifremer.fr/doc/00003/11421/

Éditeur(s) : HAL CCSD European Geosciences Union (EGU)
Résumé : International audience
Holocene climate fluctuations and human activity since the Neolithic have shaped present-day Mediter-ranean environments. Separating anthropogenic effects from climatic impacts to better understand Mediterranean pale-oenvironmental changes over the last millennia remains a challenging issue. High-resolution pollen analyses were un-dertaken on two cores from the Palavasian lagoon system (Hérault, southern France). These records allow reconstruction of vegetation dynamics over the last 4500 years. Results are compared with climatic, historical and archeological archives. A long-term aridification trend is highlighted during the late Holocene, and three superimposed arid events are recorded at 4600–4300, 2800–2400 and 1300–1100 cal BP. These periods of high-frequency climate variability coincide in time with the rapid climatic events observed in the Atlantic Ocean (Bond et al., 2001). From the Bronze Age (4000 cal BP) to the end of the Iron Age (around 2000 cal BP), the spread of sclerophyllous taxa and loss of forest cover result from anthropogenic impact. Classical Antiquity is characterized by a major reforestation event related to the concentration of rural activity and populations in coastal plains leading to forest recovery in the mountains. A major regional deforestation occurred at the beginning of the High Middle Ages. Around 1000 cal BP, forest cover is minimal while the cover of olive, chestnut and walnut expands in relation to increasing human influence. The present-day vegetation dominated by Mediterranean shrubland and pines has been in existence since the beginning of the 20th century.
ISSN: 1814-9324

hal-01447627
https://hal-univ-tlse2.archives-ouvertes.fr/hal-01447627
https://hal-univ-tlse2.archives-ouvertes.fr/hal-01447627/document
https://hal-univ-tlse2.archives-ouvertes.fr/hal-01447627/file/Azuara%20et%20al%202015_cor.pdf
DOI : 10.5194/cp-11-1769-2015

Éditeur(s) : HAL CCSD
Résumé : International audience
The plastic component bisphenol A (BPA) is suspected to exert deleterious effects towards human health and targets various cellular and molecular pathways, including activity of ATP-binding cassette drug transporters. The present study was designed to determine whether BPA and some derivatives, like its substitutes bisphenol F (BPF) and bisphenol S (BPS) and the flame retardant tetrabromobisphenol A (TBBPA), may additionally interact with solute carrier (SLC) drug transporters. Activities of the various following SLC transporters were inhibited in a major way (by > 60%) by 100 μM bisphenols: OCT1 and MATE1 (by BPA and TBBPA), OATP1B1 (by BPA, BPF and TBBPA), OATP1B3 and NTCP (by TBBPA) and OAT3 (by BPA, BPF, BPS and TBBPA); by contrast, activities of other transporters were not impacted (MATE2-K) or were stimulated (notably OCT1 by BPS and OCT2 by BPF). Transporter inhibitions due to bisphenols were concentrations-dependent, with half maximal inhibitory concentrations (IC50) ranging from 0.5 μM to 73.5 μM. BPA was finally shown to be not transported by OAT3, although inhibiting this transporter in a competitive manner. Taken together, these data indicate that bisphenols interact with SLC transporters, at concentration levels however rather higher than those occurring in humans in response to environmental exposure. © 2016 Elsevier B.V.
Toxicology in Vitro

hal-01467545
https://hal-univ-rennes1.archives-ouvertes.fr/hal-01467545
DOI : 10.1016/j.tiv.2016.12.009
PUBMED : 27989701

Éditeur(s) : HAL CCSD Frontiers
Résumé : International audience
Important set of studies have demonstrated the endocrine disrupting activity of Bisphenol A (BPA). The present work aimed at defining estrogenic-like activity of several BPA structural analogs, including BPS, BPF, BPAF, and BPAP, on 4- or 7-day post-fertilization (dpf) zebrafish larva as an in vivo model. We measured the induction level of the estrogen-sensitive marker cyp19a1b gene (Aromatase B), expressed in the brain, using three different in situ/in vivo strategies: (1) Quantification of cyp19a1b transcripts using RT-qPCR in wild type 7-dpf larva brains exposed to bisphenols; (2) Detection and distribution of cyp19a1b transcripts using in situ hybridization on 7-dpf brain sections (hypothalamus); and (3) Quantification of the cyp19a1b promoter activity in live cyp19a1b-GFP transgenic zebrafish (EASZY assay) at 4-dpf larval stage. These three different experimental approaches demonstrated that BPS, BPF, or BPAF exposure, similarly to BPA, significantly activates the expression of the estrogenic marker in the brain of developing zebrafish. In vitro experiments using both reporter gene assay in a glial cell context and competitive ligand binding assays strongly suggested that up-regulation of cyp19a1b is largely mediated by the zebrafish estrogen nuclear receptor alpha (zfERα). Importantly, and in contrast to other tested bisphenol A analogs, the bisphenol AP (BPAP) did not show estrogenic activity in our model
ISSN: 1662-4548

hal-01299976
https://hal-univ-rennes1.archives-ouvertes.fr/hal-01299976
PUBMED : 27047331
DOI : 10.3389/fnins.2016.00112
PUBMEDCENTRAL : PMC4805609

Éditeur(s) : HAL CCSD Springer Verlag
Résumé : The activity of the Damavand volcano (Central Alborz, northern Iran) began 1.8 Ma ago and continued up to 7 ka BP. Although the volcanic suite is clearly of shoshonitic affinity, only two petrographic types can be distinguished in the studied lavas: (1) weakly differentiated absarokites (49 < %SiO2 < 51), scattered around the volcano but with a regional extension, (2) highly differentiated banakites (59 < %SiO2 < 63), which form the bulk of the 4,000 m thick volcanic pile. All lavas are alkalic (3.7 < %K2O < 5), REE and LILE-rich (e.g., 85 < La < 148 ppm; 9 < Th < 32 ppm) and show highly fractionated REE patterns (69 < La/Yb < 115) and pronounced Nb-Ta negative anomalies. The absarokites are characterised by Sr (0.7045-0.7046) and Nd (0.51266-0.51269) isotope compositions close to the Bulk Earth values, and distinct from those of the banakites (0.7047 < Sr-87/Sr-86 < 0.7049, 0.51258 < Nd-143/Nd-144 < 0.51262). The Pb isotope ratios are also slightly lower in the absarokites than in the banakites (18.71 < Pb-206/Pb-204 < 18.77, 15.62 < Pb-207/Pb-204 < 15.63, 38.85 < Pb-208/Pb-204 < 38.91, and 18.77 < Pb-206/Pb-204 < 18.84, 15.62 < Pb-207/Pb-204 < 15.64, 38.94 < Pb-208/Pb-204 < 39.06, respectively). Overall, there is a clear tendency towards higher Sr, Pb and lower Nd isotope ratios with increasing degree of differentiation. This study suggests that the absarokites result from a low degree of partial melting (similar to 5%) of a highly metasomatized mantle source, which inherited its characteristics from an old subduction setting. The initiation of volcanic activity 1.8 Ma ago results from variations in the lithospheric thermal regime, probably related to lithospheric delamination as proposed for Anatolia (Pearce et al. 1990). The banakites are mainly generated by extensive fractional crystallisation (similar to 70%) of the absarokitic magma, with a limited amount (a few percents) of assimilation of an old crustal component, in the form of bulk assimilation or AFC processes, which both can explain the Sr, Nd and Pb isotope data.
ISSN: 1437-3254

hal-00411553
https://hal.archives-ouvertes.fr/hal-00411553
DOI : 10.1007/s00531-006-0159-6

Éditeur(s) : HAL CCSD Public Library of Science
Résumé : International audience
The results from recent studies show that some benzophenones (BPs) and their hydroxylated metabolites can function as weak estrogens (E2) in the environment. However, little is known about the structure-activity relationship of these molecules. We have examined the effects of exposure to ten different BPs on the proliferation of estrogen receptor (ER)-positive breast cancer cells and on the transcriptional activity of E2-target genes. We analyzed two genes that are tightly linked with estrogen-mediated proliferation, the CXCL12 and amphiregulin genes and two classical estrogen-responsive genes, the pS2 and progesterone receptor. Significant differences in the BPs efficiency to induce cell proliferation and endogenous E2-target gene expressions were observed. Using ERE-, Sp1-, AP1- and C3-reporter genes that contain different ER-binding sites in their promoter, we also showed significant differences in the BPs efficiency in activation of the ER transactivation. Together, our analyzes showed that the most active molecule is 4-hydroxy-BP. Docking analysis of the interaction of BPs in the ligand-binding pocket of ERα suggests that the minimum structural requirement for the estrogenic activity of BPs is a hydroxyl (OH) group in the phenyl A-ring that allows interaction with Glu-353, Arg-394 or Phe-404, which enhances the stability between BPs and ERα. Our modeling also indicates a loss of interaction between the OH groups of the phenyl B-ring and His-524. In addition, the presence of some OH groups in the phenyl B-ring can create repulsion forces, which may constrain helix 12 in an unfavorable position, explaining the differential estrogenic effects of BPs. These results, together with our analysis of BPs for their potency in activation of cell proliferation and ER-mediated transcription, report an improved understanding of the mechanism and structure-activity relationship of BPs.
ISSN: 1932-6203

inserm-00838323
http://www.hal.inserm.fr/inserm-00838323
http://www.hal.inserm.fr/inserm-00838323/document
http://www.hal.inserm.fr/inserm-00838323/file/journal.pone.0060567.pdf
DOI : 10.1371/journal.pone.0060567

Éditeur(s) : HAL CCSD Public Library of Science
Résumé : International audience
DNase I requires Ca²+ and Mg²+ for hydrolyzing double-stranded DNA. However, the number and the location of DNase I ion-binding sites remain unclear, as well as the role of these counter-ions. Using molecular dynamics simulations, we show that bovine pancreatic (bp) DNase I contains four ion-binding pockets. Two of them strongly bind Ca²+ while the other two sites coordinate Mg²+. These theoretical results are strongly supported by revisiting crystallographic structures that contain bpDNase I. One Ca²+ stabilizes the functional DNase I structure. The presence of Mg²+ in close vicinity to the catalytic pocket of bpDNase I reinforces the idea of a cation-assisted hydrolytic mechanism. Importantly, Poisson-Boltzmann-type electrostatic potential calculations demonstrate that the divalent cations collectively control the electrostatic fit between bpDNase I and DNA. These results improve our understanding of the essential role of cations in the biological function of bpDNase I. The high degree of conservation of the amino acids involved in the identified cation-binding sites across DNase I and DNase I-like proteins from various species suggests that our findings generally apply to all DNase I-DNA interactions.
ISSN: 1553-734X

hal-00645165
https://hal.archives-ouvertes.fr/hal-00645165
https://hal.archives-ouvertes.fr/hal-00645165/document
https://hal.archives-ouvertes.fr/hal-00645165/file/journal.pcbi.1001000.pdf
DOI : 10.1371/journal.pcbi.1001000

Éditeur(s) : HAL CCSD SAGE Publications
Résumé : International audience
A multiproxy analysis of lacustrine sediments cored in Grand-Case Pond at Saint-Martin, north of the Lesser Antilles archipelago, reveals three distinct climatic periods for the last 3700 years. From 3700 to ~2500 yr cal. BP and from 1150 yr cal. BP to the present, carbonate mud deposition occurred in connection with pond lowstands. These periods were also punctuated by severe drought events, marked by gypsum laminae, and hurricane landfalls, leading to marine sand inputs into the pond. The intermediate time interval, from 2500 to 1150 yr cal. BP, is typified by black organic mud deposition, suggesting that hypoxic to anoxic conditions prevailed at the pond bottom. These were probably linked with a perennial pond highstand and reflect more uniform and wetter climatic conditions than today. The carbon isotopic composition of the ostracod Perissocytheridea bisulcata shows that the lowest δ13C values are recorded during the hypoxic periods, as a consequence of bacterial recycling of isotopically depleted organic matter. Such a climatic history agrees closely with that documented from other records in the Caribbean area, such as the Cariaco Basin, central coast of Belize or Barbados. By constrast, discrepancies seem to emerge from the comparison between hurricane activity recorded at Saint-Martin on the one hand and Vieques (Puerto Rico) on the other hand. We explain this apparent contradiction by a balance between two distinct storm paths in response to latitudinal shifts of the Intertropical Convergence Zone (ITCZ). Stronger storm activity over the Gulf coast and the inner Caribbean Sea is favoured by a southern position of the ITCZ in connection with dry climatic conditions. Plausible links with the North Atlantic Oscillation (NAO) are also suggested.
ISSN: 0959-6836

halshs-00758748
https://halshs.archives-ouvertes.fr/halshs-00758748
https://halshs.archives-ouvertes.fr/halshs-00758748/document
https://halshs.archives-ouvertes.fr/halshs-00758748/file/Malaize_et_al._2011_cor.pdf
DOI : 10.1177/0959683611400198

Éditeur(s) : HAL CCSD Elsevier
Résumé : International audience
CXCL-test is a method that uses the estrogen-dependent secretion of the natural endogenous chemokine CXCL12 to evaluate the estrogenic activity of molecules. CXCL12 chemokine is involved in the estrogen dependent proliferation of breast cancer cells. Its measure is an indicator of cell proliferation and is used as an alternative test to classical proliferation test. Here we aimed to optimize this test, first to increase the number of tested molecules in a single assay and then to decrease the number of intermediate steps. The optimized CXCL-test was finally used for the evaluation of the estrogenic potency of emerging chemical pollutants: the UV filter benzophenones (BPs). The effect of BPs on CXCL12 secretion was also validated by real time quantitative RT-PCR. The optimized CXCL-test allowed a fast and direct assessment of estrogenic potency of molecules. The estrogenic activities of benzophenones were characterized and divided in two groups. The first one contains weak estrogenic compounds (BP, BP1, BP2, BP3, 234BP and 2344′BP). The second one contains medium estrogenic compounds (4BP, 44′BP, BP8, THB).
ISSN: 0045-6535

hal-01477205
https://hal-univ-rennes1.archives-ouvertes.fr/hal-01477205
https://hal-univ-rennes1.archives-ouvertes.fr/hal-01477205/document
https://hal-univ-rennes1.archives-ouvertes.fr/hal-01477205/file/Rapid%20assessment%20of%20estrogenic%20compounds_accepted.pdf
DOI : 10.1016/j.chemosphere.2017.01.037

Éditeur(s) : HAL CCSD
Résumé : Based on fieldwork and modern analytical technics, this work presents an overview of the Mid-Cretaceous hyper-extended rift system exposed in the northern part of the Pyrenean mountain range. Its inversion during the Pyrenean orogeny allows for precious observations of the deep-seated processes occurring at the foot of distal margins. Some peculiar aspects characterizing the pre-Alpine hyper-extended domain are examined throughout the 400km long suture that is fossilized in the North Pyrenean Zone (NPZ). The High Temperature/Low Pressure (HT/LP) thermal imprint of the extensional event is studied thanks to a dataset of more than hundred peak temperature measurement by Raman spectroscopy of the carbonaceous material (RSCM). The pre- and syn-rift metasediments are characterized by an intense ductile deformation. Field and EBSD investigations of the mechanisms of deformation reveal ductile and syn-metamorphic processes. The emplacement conditions of the peridotite into crustal levels and localized exhumation is examined in the key-localities of Urdach, Saraillé-Tos de la Coustette, Moncaup, Lherz, Caussou-Bestiac and Salvezines, The results indicate an early boudinage of the crust during the rifting process, resulting in the direct superposition of pre-rift sediments on top of mantle peridotites. Field, textural and isotopic characterization of the various ophicalcite and breccias found in the vicinity of the peridotite outcrops allow to decipher their exhumation history. New Ar-Ar geochronological results allow to precise the temporal evolution of the NPZ.
Cette thèse présente une analyse structurale et thermique des marges passives crétacées inversées dans la Zone Nord Pyrénéenne (ZNP). L'application du paléothermomètre RSCM (spectrométrie Raman de la matière carbonée) sur plus de cent échantillons répartis à travers la ZNP permet de préciser l'évolution thermique de la déchirure continentale. Un métamorphisme de haute température (450 à >600°C) se développe particulièrement au droit de zones ou la croûte continentale est extrêmement amincie voire éliminée. Les sédiments pré- et syn-rift sont marqués par une intense déformation ductile. L'analyse à l'EBSD des fabriques minérales indique une déformation de Haute-température contemporaine du métamorphisme HT-BP. Les conditions de mise en place crustale des péridotites et leur exhumation localisée sont examinées dans les localités d'Urdach, Saraillé-Tos de la Coustette, Moncaup, Lherz, Caussou-Bestiac et Salvezines. Les résultats indiquent un boudinage de la croûte continentale au cours du processus de rifting. Des décollements de couverture, principalement localisés dans les évaporites triasiques conduisent à la superposition directe des sédiments pré-rift sur le manteau exhumé. La notion nouvelle de soutirage crustal est introduite pour désigner ce processus dans lequel l'écoulement gravitaire ne joue pas un rôle principal. La caractérisation sédimentologique, pétrographique et isotopique (O et C) des différentes formations de brèches et ophicalcites observées à proximité des corps de péridotites permet de reconstituer les étapes de l'exhumation du manteau en pied de marge. Enfin de nouvelles datations permettent de mieux contraindre le calendrier des évènements successifs.
https://tel.archives-ouvertes.fr/tel-00787952

tel-00787952
https://tel.archives-ouvertes.fr/tel-00787952
https://tel.archives-ouvertes.fr/tel-00787952/document
https://tel.archives-ouvertes.fr/tel-00787952/file/TheI_se-Clerc-2012.pdf

Éditeur(s) : Elsevier
Résumé : We report on a multidisciplinary study of cold seeps explored in the Central Nile deep-sea fan of the Egyptian margin. Our approach combines in situ seafloor observation, geophysics, sedimentological data, measurement of bottom-water methane anomalies, pore-water and sediment geochemistry, and Th-230/U dating of authigenic carbonates. Two areas were investigated, which correspond to different sedimentary provinces. The lower slope, at similar to 2100 m water depth, indicates deformation of sediments by gravitational processes, exhibiting slope-parallel elongated ridges and seafloor depressions. In contrast, the middle slope, at similar to 1650 m water depth, exhibits a series of debris-flow deposits not remobilized by post-depositional gravity processes. Significant differences exist between fluid-escape structures from the two studied areas. At the lower slope, methane anomalies were detected in bottom-waters above the depressions, whereas the adjacent ridges show a frequent coverage of fractured carbonate pavements associated with chemosynthetic vent communities. Carbonate U/Th age dates (similar to 8 kyr BP), pore-water sulphate and solid phase sediment data suggest that seepage activity at those carbonate ridges has decreased over the recent past. In contrast, large (similar to 1 km(2)) carbonate-paved areas were discovered in the middle slope, with U/Th isotope evidence for ongoing carbonate precipitation during the Late Holocene (since similar to 5 kyr BP at least). Our results suggest that fluid venting is closely related to sediment deformation in the Central Nile margin. It is proposed that slope instability leads to focused fluid flow in the lower slope and exposure of 'fossil' carbonate ridges, whereas pervasive diffuse flow prevails at the unfailed middle slope.
Marine Geology (0025-3227) (Elsevier), 2009-06 , Vol. 261 , N. 1-4 , P. 92-104

Droits : 2009 Elsevier B.V. All rights reserved.
http://archimer.ifremer.fr/doc/2009/publication-6586.pdf
DOI:10.1016/j.margeo.2008.10.008
http://archimer.ifremer.fr/doc/00000/6586/

Éditeur(s) : Actes de colloques Ifremer, Tahiti, French Polynesia, 20 Feb - 4 Mar 1989, n°9, chap. 13, pp.113-126
Résumé : At least six virus diseases are presently known in cultured penaeid shrimp. Each of these six penaeid virus diseases (BP = Baculovirus penaei ; MBV = Penaeus monodon -type baculovirus (including PBV of Australian penaeids); BMN = baculoviral midgut gland necrosis; HPV = hepatopancreatic parvo-like virus; IHHNV = infectious hypodermal and hematopoietic necrosis virus; and REO = reo-like virus of the hepatopancreas) may be comprised by a multitude of individual strains, some of which are highly pathogenic to some penaeids, while being of little importance to others.

Droits : info:eu-repo/semantics/openAccess
http://archimer.ifremer.fr/doc/1989/acte-1481.pdf
http://archimer.ifremer.fr/doc/00000/1481/

Éditeur(s) : HAL CCSD Elsevier
Résumé : International audience
Multiproxy analyses were performed on core MS27PT recovered in hemipelagic sediments deposited on the Nile margin in order to reconstruct Nile River palaeohydrological fluctuations during the last 100,000 years. The strontium and neodymium isotope composition of the terrigenous fraction and the major element distribution reveal large and abrupt changes in source, oscillating between a dominant aeolian Saharan contribution during arid periods and a dominant Nile River contribution during pluvial periods. Iron content shows a strong correlation with strontium and neodymium isotopes. This allows the use of a high-resolution continuous Fe record as a proxy of Blue Nile sediment input over the last 100,000 years. The detailed Fe record, with approximately 10 years resolution during pluvial periods, is consistent with subtropical African records of well-dated lake level fluctuations and thus constitutes a first continuous high resolution record of the East African monsoon regime intensity over Ethiopia.;The detailed Fe record shows the two main known pluvial periods attributed to strengthening of the African monsoon over Ethiopia, the Nabtian period from 14 to 8 ka cal BP and the Saharan period from 98 to 72 ka BR For the first time, the last glacial period (Marine Isotope Stage (MIS) 2,3 and 4) is documented with a continuous record showing large oscillations between high and low East African palaeo-monsoon regimes.;The end of the Nabtian period occurred at 8 ka in core MS27PT, i.e. much earlier than on the East Equatorial African region where it ended around 5.5 ka. We interpret this as evidence that the southward shift of the rain belt occurred 3000 years earlier over the Eastern Ethiopian Highland and propose that the gradual southward migration of the rain belt was associated with highly variable intensity and longer rainy seasons from 8 to 5 ka. During the last glacial period, two wet periods are present around 60-50 ka BP and 38-30 ka BP. These two humid periods are in phase with the rise of atmospheric CH4 concentrations suggesting that wetland tropical African area was one of the sources of the atmospheric CH4 during the MIS 3. During the Last Glacial Maximum and MIS 4, high Saharan aeolian influxes in phase with records of aeolian dust deposited in East Antarctica are documented. This study highlights the importance of reconstructions of monsoon rainfall fluctuation at high temporal resolution to better understand the link between low- and high-latitude climate variability at millennial timescales.
ISSN: 0277-3791

hal-00496481
https://hal.archives-ouvertes.fr/hal-00496481
DOI : 10.1016/j.quascirev.2010.02.006

Éditeur(s) : HAL CCSD Elsevier
Résumé : International audience
Endocrine disrupting chemicals and especially oestrogen receptor (ER) agonists have been extensively studied over the years due to their potential effects on sexual development and reproduction in vertebrates, notably fish. As ER agonists can exist as complex mixtures in the aquatic environment, evaluating the impact of combined exposure on oestrogenic effects has become increasingly important. Use of predictive models such as concentration addition (CA) and independent action (IA) has allowed assessment of combined estrogenic effects of complex multi-compound mixtures of ER agonists in various fish in vitro and in vivo experimental models. The present work makes use of a transgenic zebrafish strain, tg(cyp19a1b-GFP), which expresses the green fluorescent protein (GFP) under the control of the cyp19a1b (brain aromatase or aromatase B) gene to determine the oestrogenic potency of ER agonists alone or in mixtures. In these studies, tg(cyp19a1b-GFP) zebrafish embryos were exposed for four days (from one to five days post fertilization) to five different oestrogenic chemicals; 17α-ethinylestradiol (EE2), 17β-estradiol (E2), estrone (E1), bisphenol A (BPA) and 4-tert-octylphenol (OP), and three mixtures of up to four of these compounds. The mixture of BPA, OP and E2 was also tested with primary cultures of rainbow trout hepatocytes by analysing the ER-mediated induction of the oestrogenic biomarker vitellogenin in order to compare the performance of the two methods for assessing oestrogenic effects of complex mixtures. The three tested mixtures were predominantly acting in an additive manner on the expression of GFP. Additivity was indicated by the overlap of the 95% confidence interval of the concentration response curves for the observed data with the CA and IA prediction models, and model deviation ratios within a factor of two for a majority of the mixture concentrations. However, minor deviations determined as more than additive effects for the mixture of EE2, E1 and E2 and less than additive effects for the mixture of BPA, OP, EE2 and E1 were observed at the higher mixture concentrations tested. The successful prediction of additivity by CA and IA in tg(cyp19a1b-GFP) zebrafish embryos and deviations at high mixture concentrations seemed to correspond well to results obtained in the rainbow trout hepatocyte assay. The present results clearly show the usefulness of combining predictive modelling and use of in vitro bioassays for rapid screening of oestrogenic effects of complex mixtures and environmental samples.
ISSN: 0166-445X

hal-00877351
https://hal.archives-ouvertes.fr/hal-00877351
DOI : 10.1016/j.aquatox.2013.05.001
PUBMED : 23721851