Éditeur(s) : CRPLC : Centre de Recherche sur les Pouvoirs Locaux dans la Caraïbe
Extrait de : "Représentations, pratiques, déconstruction du genre dans les sociétés caribéennes" : journée d'étude, le 14 novembre 2016. Université des Antilles
Description : Chercheuse en information et communication, Nathalie Almar questionne les effets des médias et la réception des informations par le public martiniquais particulièrement au sujet du rôle des médias sur la socialisation de genre. L'analyse de Nathalie Almar s'intéresse aux discours sociaux en interaction autour de l'identité féminine et plus globalement aux rôles de genres. Elle explique qu'à partir des années 1980, la représentation médiatique est constitutive du réel qu'elle produit et dont elle rend légitime les normes. En effet à cette époque, les travaux de Stuart Hall révèlent un récepteur actif, opérant un décodage en fonction de la lecture prescrite du message médiatique. Selon cette approche de la représentation qui est perçue comme une négociation qui s'interroge sur le processus de production des identités féminines dans le contexte des Antilles françaises, Nathalie Almar situe son propos dans la théorie d'incubation culturelle de George Gerbner. Selon elle, ceci évoque un processus de culture ou d'incubation des normes diffusées par les médias.
Siècle(s) traité(s) : 21
Droits : CC-BY-NC-ND - Attribution - Pas d'utilisation commerciale - Pas de modification
Permalien : http://www.manioc.org/fichiers/V16328
V16328

Éditeur(s) : Actes de colloques. Ifremer Brest [ACTES COLLOQ. IFREMER.]. 1986
Résumé : Bacterial density of the coelenteric fluid (CF) of some anthozoan species was determined in situ and under laboratory conditions. In all species examined bacterial density of the CF was higher in the gastral cavity than in ambient ranging from 10 super(5) up to 5 x 10 super(6) cells. ml super(-1). Incubation experiments with the CF indicate that a bacterial population within the coelenteron is efficiently controlled by the CF, favoring bacterial growth if bacterial densities are low in the CF and showing clearance activity at high bacterial densities. Feeding of pre-starved anthozoans caused a rapid response of coelenteric rod-shaped bacteria. Evidence is presented that coelenteric bacteria are digested periodically although they contribute 1% to the respiratory carbon losses of the antozoans tested. Therefore, it is more likely that the coelenteric bacterial population provide other substances which cannot be synthesized by the host.
La densité des bactéries du fluide gastrique de différents anthozoaires a été déterminée in situ et au laboratoire. Pour toutes les espèces étudiées, la densité des bactéries dans le fluide gastrique était plus élevée que dans l'eau ambiante et comprise entre 105et 106 cellules m"1. Des expériences d'incubation du fluide gastrique montrent que la population bactérienne est contrôlée efficacement par ce fluide gastrique, favorisant la croissance de bactéries lorsque la densité est faible mais révélant des activités de digestion lorsque la densité est élevée. En nourrissant des anthozoaires mis àjeun plusieurs jours, on provoque une réaction rapide des bactéries déforme bacillaire. Certaines observations montrent que les bactéries gastriques se développent et sont digérées par cycle dans le coelenteron. Néanmoins, la contribution en carbone de ces dernières, comparée aux pertes d'énergie par Sa respiration, représenterait moins de i ','-£. li est probable que cette population bactérienne fournisse des substances qui ne peuvent être synthétisées par i'hôte.

Droits : info:eu-repo/semantics/openAccess
http://archimer.ifremer.fr/doc/1984/acte-989.pdf
http://archimer.ifremer.fr/doc/00000/989/

Éditeur(s) : HAL CCSD Public Library of Science
Résumé : International audience
We assessed the occurrence of algae in five species of tank-bromeliads found in contrasting environmental sites in a Neotropical, primary rainforest around the Nouragues Research Station, French Guiana. The distributions of both algal abundance and biomass were examined based on physical parameters, the morphological characteristics of bromeliad species and with regard to the structure of other aquatic microbial communities held in the tanks. Algae were retrieved in all of the bromeliad species with mean densities ranging from ∼10(2) to 10(4) cells/mL. Their biomass was positively correlated to light exposure and bacterial biomass. Algae represented a tiny component of the detrital food web in shaded bromeliads but accounted for up to 30 percent of the living microbial carbon in the tanks of Catopsis berteroniana, located in a highly exposed area. Thus, while nutrient supplies are believed to originate from wind-borne particles and trapped insects (i.e., allochtonous organic matter), our results indicate that primary producers (i.e., autochtonous organic matter) are present in this insectivorous bromeliad. Using a 24-h incubation of size-fractionated and manipulated samples from this plant, we evaluated the impact of mosquito foraging on algae, other microorganisms and rotifers. The prey assemblages were greatly altered by the predation of mosquito larvae. Grazing losses indicated that the dominant algal taxon, Bumilleriopsis sp., like protozoa and rotifers, is a significant part of the diet of mosquito larvae. We conclude that algae are a relevant functional community of the aquatic food web in C. berteroniana and might form the basis of a complementary non-detrital food web.
ISSN: 1932-6203

hal-00819161
https://hal.archives-ouvertes.fr/hal-00819161
https://hal.archives-ouvertes.fr/hal-00819161/document
https://hal.archives-ouvertes.fr/hal-00819161/file/Brouard2011_AreAlgae.pdf
DOI : 10.1371/journal.pone.0020129

Résumé : L’objectif de ce travail est la définition d’une méthode d’incubation des oeufs de platax orbicularis garantissant une estimation plus précise et plus sûre du nombre d’animaux réalisée au démarrage des élevages larvaires Afin de pouvoir améliorer la précision de la répartition, nous avons fait l’hypothèse qu’il est préférable de mettre en place une incubation des oeufs directement dans les bassins d’élevage. En effet les oeufs sont plus facilement manipulables que les larves, ce qui présente un avantage important lors de leur comptage. Cette méthode faciliterait aussi la conduite de certains protocoles d’élevage larvaire, notamment la production de familles. Un gain de qualité des larves est aussi attendu découlant du fait que les larves ne sont plus manipulées après l’éclosion. Mais cette méthode présente l’inconvénient de ne pas pouvoir connaître directement le taux d’éclosion et l’effectif post-éclosion présent dans les bassins. Il est donc nécessaire de mettre en place en parallèle une méthode d’incubation témoin. Les résultats de comptage montrent qu’il est plus sur de compté des oeufs que des larves et six prélèvements de 5ml sont suffisant pour avoir une précision de 8 % et cela de façon reproductible. La mise en place d’éclosion témoin en incubateur de 50 litres a permis d’estimer l’éclosion avec un intervalle de confiance de 3 %. Ce qui a permis la mise en place de la méthode « directe » engendrant un gain de précision dans la répartition de l’ordre de 40 %. Au final, il serait préférable de compter les oeufs, puis de les répartir par volumétrie dans les bassins d’élevage. Il est préférable d’utiliser en parallèle des incubateurs de 50 litres pour conduire une incubation témoin précise et sûre. Les taux d’éclosion de ces témoins seront appliqués aux bassins larvaires et permettront de connaître la population de chaque bassin.

Droits : info:eu-repo/semantics/openAccess
http://archimer.ifremer.fr/doc/00128/23877/21812.pdf
http://archimer.ifremer.fr/doc/00128/23877/

Éditeur(s) : Actes de colloques. Ifremer Brest [ACTES COLLOQ. IFREMER.]. 1986
Résumé : Using in situ incubations in dialysis bags of natural bacterial communities, the change in growth rates over a 2-day period has been studied for the community as a whole and for different morphological groups within the community. A sequence of events during growth of both communities well above (4m) and well below (60m) the thermocline in the stratified waters, close to a shallow sea front in the Irish Sea, was noted. There was evidence not only of a periodic pattern of growth of the whole bacterial community but also of synchronous cell division of groups within the community. An increase in mean cell volume of both 4 m and 60 m bacterial communities preceded an increase in specific growth rate which usually coincided with a decrease in mean cell volume. There are distinct differences in numbers of phytoplankton, protozooplankton, macrozooplankton and fish between the waters above and below the thermocline. Some of these organisms exhibit diurnal rhythms and their possible influence on the growth rates and rhythms of bacterial communities in each water mass is discussed.
Les modifications du taux de croissance d'une communauté naturelle de bactéries, prise dans son ensemble, et des différents groupes morphologiques la constituant sont étudiées sur une période de 2 jours par incubations in situ dans des sacs à dialyse. Les phénomènes successifs survenant durant la croissance de la communauté sont étudiés à la fois nettement au-dessus (4m) et au-dessous (60m) de la thermocline dans des eaux stratifiées, à proximité d'un front côtier en mer d'Irlande. Il est mis en évidence, non seulement des phases périodiques de croissance sur toute la communauté bactérienne, mais également une division synchrone des cellules dans les différents groupes de la communauté. Une augmentation du volume moyen des cellules des deux communautés des 4 et 60 m précède une augmentation du taux de croissance spécifique, qui coïncide généralement avec une diminution du volume cellulaire moyen. La communauté présente à 4 m, dans son ensemble, mais aussi les deux groupes morphologiques (cocci et bâtonnets) qui la composent, ont des temps de génération proches de la journée. Toutefois, la plupart des formes coccoides se divisent pendant la nuit, et la plupart des formes en bâtonnet se divisent pendant la journée. Réciproquement, à 60 m, la plupart des bâtonnets se divisent pendant la nuit, tout en ayant le même temps de génération qu'à 4 m. La plupart des cocci se divisent pendant la journée et ont un temps degeneration plus faible (presque trois jours). Ceci fait passer le temps de génération de l'ensemble de la communauté des 60 m à 2 jours. Des différences nettes sont observées dans les numérations de phytoplancton, protozooplancton, macrozooplancton et poissons entre les eaux situées au-dessus et au dessous de la thermocline. Plusieurs de ces organismes présentent des rythmes diurnes et leur influence possible sur les taux de croissance et rythmes des communautés bactériennes dans chacune de ces masses d'eau est discutée.

Droits : info:eu-repo/semantics/openAccess
http://archimer.ifremer.fr/doc/1984/acte-951.pdf
http://archimer.ifremer.fr/doc/00000/951/

Éditeur(s) : Edp Sciences S A
Résumé : Sperm characteristics of scallops have not been well described in the scientific literature. The effects of sperm release technique (thermal shock versus serotonin injection), of sperm collection technique (testis sampling versus serotonin injection), of sperm sampling location along the genital tract, of in vitro sperm maturation, and of time post activation on scallop sperm characteristics were assessed in the present work. Whatever sperm release technique used, no significant differences were observed regarding the percentage of motile spermatozoa and the velocity of the average path (YAP). Compared to testicular sperm, a higher percentage of motile spermatozoa, YAP and intracellular adenosine triphosphate (ATP) content were observed for sperm shed after serotonin injection. From the distal part of testes up to the gonopore, an increase of the percentage of motile spermatozoa and YAP was assessed, suggesting a sperm 'maturation process' along the genital ducts. A higher increase in the percentage of motile sperm was recorded during a 5 mm incubation of testicular sperm in seawater containing 2 mM serotonin and seawater containing 10 mM caffein compared to seawater (control). In addition, a higher YAP was assessed, incubating testicular sperm in caffein, compared to control or serotonin. Then, the percentage of motile spermatozoa, YAP and intracellular ATP content exhibited a progressive reduction during the 10 h swimming period. Mean values of the percentage of motile spermatozoa, YAP, sperm track linearity (LIN) and intracellular ATP content recorded at the beginning of the movement period for sperm samples collected after intragonadal serotonin injection, were 82 +/- 7%, 162 +/- 15 mu m s(-1), 0.33 +/- 0.12 and 212 +/- 133 nmol x 10(-9) spermatozoa (n = 9 males), respectively. The present study confirms the existence of a sperm "maturation process" along scallop genital ducts. In addition, the cessation of scallop sperm movement can be explained by the exhaustion of ATP content at the end of the movement phase.
Aquatic Living Resources (0990-7440) (Edp Sciences S A), 2013-07 , Vol. 26 , N. 3 , P. 215-220

Droits : EDP Sciences, IFREMER, IRD 2013
http://archimer.ifremer.fr/doc/00157/26852/24983.pdf
DOI:10.1051/alr/2013048
http://archimer.ifremer.fr/doc/00157/26852/

Résumé : Seawater, algae, micro-diets and Artemia are the main inputs in shrimp larval rearing. Each of them are potential carriers of pathogenic bacteria in the rearing media and weaken the animal in its early life stages. Daily monitoring of rearing water and larvae showed a bacterial spike after the first live feed meal. Vibrio settlement can be enhancedby giving Artemia with their incubation water where vibrio concentration can reach up to 105 CFU per milliliter. This study showed that simple measures such as rinsing and supplying Artemia with freshwater allowed a significant drop in their bacterial load.
L’élevage larvaire de crevettes nécessite l’utilisation de plusieurs intrants que sont l’eau de mer, les algues, les micro-particules et les Artemia. Chacun de ces intrants peut être une porte d’entrée potentielle de bactéries pathogènes et affaiblir l’animal dans les stades précoces. Des analyses bactériennes de l’eau d’élevage et des larves au quotidien montrent que c’est après l’introduction des proies vivantes que la flambée bactérienne est observée. La présence de vibrio peut être favorisée par la distribution des artémies dans leur eau d’incubation où il a pu être dénombré jusqu’à 105 CFU/ml. Cette fiche biotechnique montre qu’une mesure simple et efficace telle que le rinçage et la distribution des proies vivantes dans l’eau douce permet de réduire significativement la quantité de bactéries apportée par les Artemia.

Droits : 2008 Ifremer
http://archimer.ifremer.fr/doc/00118/22971/20801.pdf
http://archimer.ifremer.fr/doc/00118/22971/

Éditeur(s) : HAL CCSD Elsevier
Résumé : International audience
Viral haemorragic fevers are characterized by similar clinical manifestations, with a biphasic course : After a 2-21 day-incubation, onset of an influenza-like illness, that will drive to an haemorragic syndrom with shock, in a subgroup of patients, unpredictable. Human-to-human transmission mostly occurs through contact with biological fluids, especially if contaminated with blood. The biology, the epidemiology and the letality of these viruses differ. For all these viral haemorragic fevers, the importance of an early diagnosis relies mostly in the need to interrupt human-to-human transmission, as therapeutic options are mostly restricted to symptomatic treatment, with the exception of Lassa fever (ribavirin probably effective).
Les fièvres hémorragiques virales regroupent plusieurs viroses, potentiellement graves, avec un tableau clinique relativement stéréotypé, d’évolution biphasique. Après une incubation de 2 à 21 jours, apparition d’un syndrome pseudo-grippal qui évolue au bout de quelques jours, de manière imprévisible, chez une proportion variable de patients, vers un syndrome hémorragique avec choc. La transmission interhumaine s’effectue le plus souvent par contacts avec des liquides biologiques, surtout en présence de sang. La biologie de ces virus, leur épidémiologie et leur létalité sont très variables. Pour toutes ces fièvres hémorragiques virales, l’importance d’un diagnostic rapide réside plus dans la nécessité d’interrompre les chaînes de transmission que dans les possibilités thérapeutiques, limitées aux traitements symptomatiques, à l’exception notable de la fièvre de Lassa (efficacité probable de la ribavirine).
ISSN: 1773-035X

hal-01299296
https://hal-univ-rennes1.archives-ouvertes.fr/hal-01299296
https://hal-univ-rennes1.archives-ouvertes.fr/hal-01299296/document
https://hal-univ-rennes1.archives-ouvertes.fr/hal-01299296/file/Fi%C3%A8vres%20h%C3%A9morragiques%20virales_accepted.pdf
DOI : 10.1016/S1773-035X(16)30091-0

Éditeur(s) : HAL CCSD
Résumé : Carbon isotopic signature has been measured at the different stages of the soil CO2 efflux production in trenched and control plots to improve the knowledge about the physical and biochemical processes of the soil respiration. All the measurements of _13C have been performed using a mass-spectrometer.We have compared the isotopic signatures of the CO2 going out of the soil, the CO2 of the soil atmosphere, the organic carbon in the material that could be considered as the main soil respiration sources (live and dead roots, soil organic matter, aerial litter) and the CO2 produced by these sources in incubation jars. The aims of this study were to evaluate the possibilities to combine these different data sets to estimate the partitioning of soil respiration between its autotrophic and heterotrophic components, and to get more insight into potential fractionation during biochemical or diffusion processes. Measurements were conducted in a beech forest located at Hesse in the North East of France (site equipped with a flux tower and belonging to the CarboEurope network) and in a neotropical primary rainforest located at Paracou (French Guiana). Preliminary results show first a very tenuous difference between CO2 isotopic signatures of efflux coming from the trenched and control plots and, secondly, _13C significant divergences between bulk organic material and CO2 produced in the corresponding incubation jars. Our ability to use this kind of data for partitioning soil respiration into its main components will be discussed.
European Geosciences Union. General Assembly 2006
Vienne, Austria

hal-01032380
https://hal.archives-ouvertes.fr/hal-01032380

Éditeur(s) : HAL CCSD
Résumé : Carbon isotopic signature has been measured at the different stages of the soil CO2 efflux production in trenched and control plots to improve the knowledge about the physical and biochemical processes of the soil respiration. All the measurements of d13C have been performed using a mass-spectrometer. We have compared the isotopic signatures of the CO2 going out of the soil, the CO2 of the soil atmosphere, the organic carbon in the material that could be considered as the main soil respiration sources (live and dead roots, soil organic matter, aerial litter) and the CO2 produced by these sources in incubation jars. The aims of this study were to evaluate the possibilities to combine these different data sets to estimate the partitioning of soil respiration between its autotrophic and heterotrophic components, and to get more insight into potential fractionation during biochemical or diffusion processes. Measurements were conducted in a beech forest located at Hesse in the North East of France (site equipped with a flux tower and belonging to the CarboEurope network) and in a neotropical primary rainforest located at Paracou (French Guiana). Preliminary results show first a very tenuous difference between CO2 isotopic signatures of efflux coming from the trenched and control plots and, secondly, d13C significant divergences between bulk organic material and CO2 produced in the corresponding incubation jars. Our ability to use this kind of data for partitioning soil respiration into its main components will be discussed.
AGU Fall Meeting
San Francisco, United States

hal-01032042
https://hal.archives-ouvertes.fr/hal-01032042

Éditeur(s) : HAL CCSD Elsevier
Résumé : We studied the evolution of the microstructure of ice-Ih during static recrystallization by stepwise annealing experiments. We alternated thermal annealing and electron backscatter diffraction (EBSD) analyses on polycrystalline columnar ice pre-deformed in uniaxial compression at temperature of −7 °C to macroscopic strains of 3.0–5.2. Annealing experiments were carried out at −5 °C and −2 °C up to a maximum of 3.25 days, typically in 5–6 steps. EBSD crystal orientation maps obtained after each annealing step permit the description of microstructural changes. Decrease in average intragranular misorientation at the sample scale and modification of the misorientation across subgrain boundaries provide evidence for recovery from the earliest stages of annealing. This initial evolution is similar for all studied samples irrespective of their initial strain or annealing temperature. After an incubation period ≥1.5 h, recovery is accompanied by recrystallization (nucleation and grain boundary migration). Grain growth proceeds at the expense of domains with high intragranular misorientations, consuming first the most misorientated parts of primary grains. Grain growth kinetics fits the parabolic growth law with grain growth exponents in the range of 2.4–4.0. Deformation-induced tilt boundaries and kink bands may slow down grain boundary migration. They are stable features during early stages of static recrystallization, only erased by normal growth, which starts after >24 h of annealing.
ISSN: 0191-8141

hal-01685644
https://hal.archives-ouvertes.fr/hal-01685644
DOI : 10.1016/j.jsg.2017.05.001

Éditeur(s) : HAL CCSD Cambridge University Press
Résumé : International audience
The liver is the most important link between the circadian system and metabolism. As a food-entrainable oscillator, the hepatic clock needs to be entrained by food-related signals. The objective of the present study was to investigate the possible role of ghrelin (an orexigenic peptide mainly synthesized in the gastrointestinal tract) as an endogenous synchronizer of the liver oscillator in teleosts. To achieve this aim, we first examined the presence of ghrelin receptors in the liver of goldfish. Then, the ghrelin regulation of clock gene expression in the goldfish liver was studied. Finally, the possible involvement of the phospholipase C/ protein kinase C (PLC/ PKC) and adenylate cyclase/protein kinase A (AC/PKA) intracellular signalling pathways was investigated. Ghrelin receptor transcripts, ghs-r1a, are present in the majority of goldfish hepatic cells. Ghrelin induced the mRNA expression of the positive (gbmal1a, gclock1a) and negative (gper genes) elements of the main loop of the molecular clock machinery, as well as grev-erba (auxiliary loop) in cultured liver. These effects were blocked, at least in part, by a ghrelin antagonist. Incubation of liver with a PLC inhibitor (U73122), a PKC activator (phorbol 12-myristate 13-acetate) and a PKC inhibitor (chelerythrine chloride) demonstrated that the PLC/ PKC pathway mediates such ghrelin actions. Experiments with an AC activator (forskolin) and a PKA inhibitor (H89) showed that grev-erba regulation could be due to activation of PKA. Taken together, the present results show for the first time in vertebrates a direct action of ghrelin on hepatic clock genes and support a role for this hormone as a temporal messenger in the entrainment of liver circadian functions.
Journal of Experimental Biology

hal-01519254
https://hal-univ-rennes1.archives-ouvertes.fr/hal-01519254
https://hal-univ-rennes1.archives-ouvertes.fr/hal-01519254/document
https://hal-univ-rennes1.archives-ouvertes.fr/hal-01519254/file/Ghrelin%20induces%20clock%20gene%20expression.pdf
DOI : 10.1242/jeb.144253

Éditeur(s) : Actes de colloques. Ifremer. Brest [ACTES COLLOQ. IFREMER.]. 1985
Résumé : Sexual differentiation of the marine leatherback turtle is sensitive to temperature. Incubation of eggs below 29 degree C results in 100% males, whereas incubationa bove 30 degree C results in 100% potential females at hatching. Therefore, the threshold temperature lies between 29 and 30 degree C as has been found for other marine turtles (Caretta caretta, Chelonia mydas ). Among hatchlings from natural nests in French Guiana, 10% were males, 20% were potential females and 70% had an intermediate phenotype. The sand temperature at 60 cm of depth, near one nest, was recorded and was found to vary between 29 and 30 degree C during the presumed thermosensitive period. These results will be useful to marine turtle hatcheries which seek to manipulate sex ratios among their neonates.

Droits : info:eu-repo/semantics/openAccess
http://archimer.ifremer.fr/doc/1983/acte-1241.pdf
http://archimer.ifremer.fr/doc/00000/1241/

Éditeur(s) : HAL CCSD Springer Verlag
Résumé : International audience
Up to now, several studies have investigated estrogen receptor (ER)–estrogen response element (ERE) interaction using biosensors such as surface plasmon resonance. These strategies have aimed to understand the molecular mechanism of such interaction as well as the effect of the ligand on this interaction. These approaches start to be used to determine the mechanisms of protein/DNA interaction, in particular in the context of drug discovery or environmental applications. However, some physical and biochemical parameters (incubation time, temperature, protease inhibitor cocktail, and bovine serum albumin (BSA)) are not completely described in the literature and could deeply modify the obtained results. This paper aims to focus not only on the preliminary steps of sample preparation such as protein thawing and incubation conditions (time and temperature) but also on the evaluation of protease inhibitor cocktail and BSA effect on the measurement of ER–ERE interactions.
ISSN: 1618-2642

hal-01418341
https://hal.archives-ouvertes.fr/hal-01418341
https://hal.archives-ouvertes.fr/hal-01418341/document
https://hal.archives-ouvertes.fr/hal-01418341/file/Estrogen%20Receptor%20preparation%20effects_accepted.pdf
DOI : 10.1007/s00216-016-9967-0

Éditeur(s) : HAL CCSD BioMed Central
Résumé : International audience
BACKGROUND: The potential interest of antifungal treatment of non-immunocompromized patients with sepsis, extra-digestive Candida colonization and multiple organ failure is unknown. It represents three-quarters of antifungals prescribed in Intensive Care Units. It may allow early treatment of invasive fungal infection in the incubation phase but expose patients to unnecessary antifungal treatments with subsequent cost and fungal selection pressure. As early diagnostic tests for invasive candidiasis are still considered to be insufficient, the potential interest in this strategy needs to be demonstrated. METHODS: This prospective multicenter, double blind, randomized-controlled trial is conducted in 23 French Intensive Care Units. All adult patients, mechanically ventilated for more than four days with sepsis of unknown origin and with at least one extradigestive fungal colonization site and multiple organ failure are eligible for randomization. Patients with proven invasive candidiasis are not included. After a complete mycological screening, patients are allocated to receive micafungin 100 mg intravenously once a day or placebo for 14 days. We plan to enroll 260 patients. The main objective is to demonstrate that micafungin increases survival of patients without invasive candidiasis at day 28 as compared to placebo. Other outcomes include day 28 and 90 survival and organ failure evolution. Additionally, pharmacokinetics of micafungin in enrolled patients will be measured and evolution of fungal biomarkers and susceptibility profiles of infecting fungi will also be followed. DISCUSSION: This study will help to provide guidelines for treating non-immunocompromized patients with fungal colonization multiple organ failure and sepsis of unknown origin.Trial registration: Clinicaltrials.gov number NCT01773876.
ISSN: 1745-6215

inserm-00913515
http://www.hal.inserm.fr/inserm-00913515
http://www.hal.inserm.fr/inserm-00913515/document
http://www.hal.inserm.fr/inserm-00913515/file/1745-6215-14-399.pdf
PUBMED : 24261608

Éditeur(s) : HAL CCSD Institute of Electrical and Electronics Engineers
Résumé : International audience
: A new set-up for exposure of human cells in vitro at 37°C to pulse-modulated 300 MHz and 500 MHz signals of future magnetic resonance imaging (MRI) systems is designed, built up, and characterized. Two dipole antennas, specifically designed for ultra high field MRI, are used as radiating structures. The electromagnetic (EM) field distribution inside the incubator containing the cells is computed, and it is shown to be in a good agreement with measurements. The electric field at the cell level is quantified numerically. Local, 1g average, and averaged over the culture medium volume SAR are provided along with the standard deviation values for each well. Temperature increments are measured inside the culture medium during the exposure using an optical fiber thermometer. Then we identify the pulse parameters corresponding to the thermal threshold of 1°C, usually considered as a threshold for thermally-induced biological effects. For these parameters, the induction of heat shock proteins is assessed to biologically verify a potential thermal response of cells. The data demonstrate that, under the considered experimental conditions, exposure to pulsemodulated radiations emulating typical ultra high field MRI signals, corresponding to temperature increments below 1°C, does not trigger any heat shock response in human brain cells.
ISSN: 0018-9294

hal-00874329
https://hal.archives-ouvertes.fr/hal-00874329
DOI : 10.1109/TBME.2013.2270371
PUBMED : 23799680

Éditeur(s) : HAL CCSD IOS Press
Résumé : International audience
Sickle cell anemia (SCA) is an inherited red blood cells (RBC) disorder characterized by significantly decreased RBC deformability. The present study aimed to assess whether modulation of RBC Nitric Oxide Synthase (RBC-NOS) activation could affect RBC deformability in SCA.Blood of twenty-five SCA patients was treated for 1 hour at 37°C with Phosphate Buffered Saline (PBS) or PBS containing 1% of Dimethylsulfoxyde as control, L-arginine or N(5)-(1-Iminoethyl)-L-ornithine (L-NIO) to directly stimulate or inhibit RBC-NOS, insulin or wortmannin to indirectly stimulate or inhibit RBC-NOS through their effects on the PI3 Kinase/Akt pathway, and sodium nitroprusside (SNP) and 2-(4-Carboxyphenyl)-4,4,5,5-tetramethylimidazoline-1-oxyl-3-oxide (cPTIO) as NO donor and NO scavenger, respectively. RBC deformability was measured by ektacytometry at 3 Pa.RBC deformability significantly increased after insulin treatment and significantly decreased after L-NIO and wortmannin incubation. The other conditions did not affect deformability. Significantly increased nitrotyrosine levels, a marker of enhanced free radical generation, were detected by immunohistochemistry in SNP and insulin treated samples.These data suggest that RBC deformability of SCA can be modulated by RBC-NOS activity but also that oxidative stress may impair effectiveness of RBC-NOS produced NO.
ISSN: 1386-0291

hal-01669738
https://hal.univ-antilles.fr/hal-01669738
DOI : 10.3233/CH-162042

Éditeur(s) : HAL CCSD Springer Verlag
Résumé : International audience
Meconium is the earliest stool of newborns, and is a complex matrix that reflects the degree of exposure of the fetus to xenobiotics. To investigate fetal exposure to volatile organic compounds, an analytical method was developed to identify and quantify BTEX (benzene, toluene, ethylbenzene, and o,m,p-xylene) and two chlorinated solvents (trichloroethylene and tetrachloroethylene) in meconium. Headspace-solid-phase microextraction coupled with gas chromatography-mass spectrometry was selected because it is simple, sensitive, can be automated, and requires no extensive sample preparation. Several extraction variables were optimized (fiber type, incubation time, temperature of fiber, and use of salt). Because meconium is a complex matrix, quantification by SPME was considered carefully because of potential interference, for example competitive adsorption. Calibration in water was compared with calibration in meconium using external and internal methods (with isotope-labeled compounds). In meconium, limits of quantification were determined to be in the range 0.064-0.096 ng g(-1) for the investigated compounds. All target compounds were determined in "real-case" meconium samples.
ISSN: 1618-2642

hal-01064967
https://hal-univ-rennes1.archives-ouvertes.fr/hal-01064967
DOI : 10.1007/s00216-014-7836-2
PUBMED : 24838489

Résumé : Cette thèse concerne la modélisation des dynamiques de maladies foliaires de cultures pérennes tropicales à différentes échelles spatiales. Cette approche de modélisation est appliquée à la cercosporiose noire du bananier. Il s’agit d’explorer et de déterminer les paramètres environnementaux et de résistance de l’hôte ayant une influence significative sur la dynamique spatiotemporelle de la maladie et d’apporter des éléments associés au contrôle de la cercosporiose noire.Deux modèles ont été développés dans le cadre de cette thèse. La dynamique épidémiologique au niveau de la plante est décrite par un modèle mécaniste décomposé en un modèle de croissance de la plante et un module épidémiologique décrivant le cycle épidémique du pathogène. L’architecture du bananier est prise en compte par le biais de compartiments foliaires positionnés dans l’espace. Le modèle a été validé par un jeu de données indépendant. Les expérimentations numériques et l’analyse de sensibilité du modèle réalisées par les méthodes de Morris et de e-FAST ont permis de mieux comprendre le fonctionnement épidémique de cette maladie et d’identifier les paramètres influant le plus la dynamique épidémique en particulier la vitesse d’extension des lésions, la durée d’incubation et l’efficacité d’infection .L’approche bayésienne a permis de prendre en compte l’information a priori disponible pour ces trois paramètres sur lesquels a porté l’inférence statistique. L’analyse de sensibilité du modèle a également permis d’identifier l’influence de deux paramètres liés à la croissance de la plante : le nombre de feuilles sur une plante et le rythme d’émission foliaire Un modèle de dynamique spatio-temporelle de la cercosporiose noire a été développé à l’échelle d’un territoire à partir d’enquêtes effectuées enMartinique pendant la période d’invasion de cette maladie de septembre 2010 à mai 2012. Les données récoltées étant censurées, l’inférence des paramètres du modèle a été réalisée dans un cadre bayésien en utilisant un algorithme d’augmentation de données. Le modèle et l’inférence développés permettent de reconstruire la dynamique spatio-temporelle de l’invasion et de prédire la fin d’invasion sur le territoire.Les deux modèles de dynamiques spatio-temporelles développés à deux échelles spatiales différentes ont permis d’acquérir des informations importantes pour construire des outils de conception de méthode de contrôle de la cercosporiose noire des bananiers.
This thesis concerns the modeling of the dynamics of foliar diseases of tropical tree crops at different spatial scales. This modeling approach is applied to black Sigatoka of banana. It is to explore and determine the environmental parameters and host resistance has a significant influence on the spatiotemporal dynamics of the disease and to provide elements associated with the control of Sigatoka noire.Deux models were developed as part of this thesis. The epidemiological dynamics at the plant is described by a mechanistic model decomposed into a growth model of the plant and an epidemiological unit describing the pathogen epidemic cycle. The architecture of the banana is taken into account through foliar compartments positioned in space. The model was validated by an independent data set. Numerical experiments and model sensitivity analysis performed by the methods of Morris and e-FAST enabled to better understand the functioning of this epidemic disease and identify the parameters affecting the most dynamic epidemic especially speed extension of the lesions, the incubation period and efficiency of Bayesian .L'approche infection allowed to take into account prior information available for these three parameters that were the statistical inference. The model sensitivity analysis also identified the influence of two parameters related to plant growth: the number of leaves on a plant and rate of leaf emission A dynamic model of space-time Black Sigatoka has been developed at the scale of a territory from surveys enMartinique during the period of invasion of the disease from September 2010 to May 2012. The data collected being censored, inference of model parameters has was performed in a Bayesian framework, using a data augmentation algorithm. The model developed and inference possible to reconstruct the spatiotemporal dynamics of the invasion and predict the end of the invasion territoire.Les two spatio-temporal dynamics models developed at two different spatial scales has been gained important information for build tools design method of control of black Sigatoka of banana.

http://www.theses.fr/2015AGUY0835/document

Éditeur(s) : HAL CCSD
Résumé : Carbon isotopic signature has been measured at the different stages of the soil CO2 efflux production in trenched and control plots to improve the knowledge about the physical and biochemical processes of the soil respiration. All the measurements of _13C have been performed using a mass-spectrometer.We have compared the isotopic signatures of the CO2 going out of the soil, the CO2 of the soil atmosphere, the organic carbon in the material that could be considered as the main soil respiration sources (live and dead roots, soil organic matter, aerial litter) and the CO2 produced by these sources in incubation jars. The aims of this study were to evaluate the possibilities to combine these different data sets to estimate the partitioning of soil respiration between its autotrophic and heterotrophic components, and to get more insight into potential fractionation during biochemical or diffusion processes. Measurements were conducted in a beech forest located at Hesse in the North East of France (site equipped with a flux tower and belonging to the CarboEurope network) and in a neotropical primary rainforest located at Paracou (French Guiana). Preliminary results show first a very tenuous difference between CO2 isotopic signatures of efflux coming from the trenched and control plots and, secondly, _13C significant divergences between bulk organic material and CO2 produced in the corresponding incubation jars. Our ability to use this kind of data for partitioning soil respiration into its main components will be discussed.
Geophysical Research Abstracts

hal-01031785
https://hal.archives-ouvertes.fr/hal-01031785