Éditeur(s) : HAL CCSD
Résumé : International audience
https://hal.archives-ouvertes.fr/hal-01625514

hal-01625514
https://hal.archives-ouvertes.fr/hal-01625514

Éditeur(s) : HAL CCSD
Résumé : International audience
https://hal.archives-ouvertes.fr/hal-01625505

hal-01625505
https://hal.archives-ouvertes.fr/hal-01625505

Éditeur(s) : HAL CCSD Elsevier Masson
Résumé : International audience
Introduction. - Temocillin is an alternative to treat urinary tract infections with bacteria producing extended spectrum beta-lactamase (ESBL). The objective is to evaluate the use of temocillin in urinary tract infections. Materials and methods. - A systematic review of literature was carried out according to PRISMA criteria. All national and international recommendations have been reviewed regarding the indication of the use of temocillin in urology. Data collection on the use of temocillin in urinary tract infection has been performed from the Cochrane, LILACS and the Medline database. The following keywords were used: temocillin, extended spectrum. beta-lactamase, urinary tract infections, urine, prostate, epididymis, testis, diffusion, pharmacokinetics, pharmacodynamics. The selection was based on the methodology, language of publication (English/French), relevance to the topic and date of publication of the articles collected. The endpoint was to provide exhaustive scientific information allowing urologists to use this antibiotic in febrile urinary infections. Results. - Bacteria producing ESBL has a relatively high susceptible to temocillin, ranging from 61 % to 90 %. These rates would allow its use in probabilistic. The dosage recommended is currently, in the normo-renal patient, 4 g per day by intermittent infusion or continuously after a loading dose of 2g. Some studies argue, particularly in case of difficult clinical situations or obese patients, for administration of high doses (6 g/24 h) rather continuous infusion. There is no evident relationship between high doses and side effects. With an excellent urinary and prostatic diffusion, temocilllin might be recommend for the treatment of ESBL prostatitis. Conclusion. - Temocillin is known to have good urinary and prostatic diffusion. According to our results, this antibiotics is now a reliable alternative for the treatment of documented ESBL urinary tract infections. (C) 2017 Elsevier Masson SAS. All rights reserved.
ISSN: 1166-7087

hal-01659245
https://hal-univ-rennes1.archives-ouvertes.fr/hal-01659245
DOI : 10.1016/j.purol.2017.07.242
PUBMED : 28869169

Éditeur(s) : HAL CCSD Wiley
Résumé : International audience
TMPRSS2/ERG fusion resulting in ERG overexpression occurs in 30 to 50% of prostate cancer (PCa) in Caucasian patients, but its prognostic relevance remains controversial. In the present study, we investigated ERG expression in all stages of PCa progression, and evaluated the prognostic impact of ERG status in clinically localized PCa (CLC) and in castration resistant disease (CRPC). ERG and AR expressions were evaluated by immunohistochemistry on tissue microarrays containing samples of high grade PIN (n = 57), CLC surgically treated (n = 299, including 185 Caucasians and 114 African-Caribbeans), metastases (n = 17), and CRPC (n = 41). In Caucasians, ERG expression significantly increased from high grade PIN (17.5%) to pT2 (27%) and pT3 CLC (43%), then to metastases (53%). In CLC, stainings for ERG and AR were correlated, and ERG expression was less frequent in African-Caribbeans compared to Caucasians (11.5% vs. 33%). In Caucasians CLC, ERG was associated with longer recurrence free survival, after adjusting for classical prognostic markers. In CRPC, ERG was expressed in 29% of cases, and was associated with a longer overall survival. Our results confirm that ERG expression is less frequent in PCa from patients of African descent. Although ERG expression increases during PCa natural history, positive ERG status is associated with better outcome in both CLC and CRPC. This paradox could be explained in part by the fact that ERG expression is AR dependant, then ERG positive cancers are likely to progress in a rich androgen environment, with a better response to androgen suppression.
ISSN: 0270-4137

hal-01118555
https://hal-univ-rennes1.archives-ouvertes.fr/hal-01118555
DOI : 10.1002/pros.22863
PUBMED : 25175352

Éditeur(s) : HAL CCSD Elsevier Masson
Résumé : National audience
o abstract
ISSN: 1166-7087

hal-01477211
https://hal-univ-rennes1.archives-ouvertes.fr/hal-01477211
DOI : 10.1016/j.purol.2016.07.209
PUBMED : 27969303

Éditeur(s) : HAL CCSD
Résumé : International audience
Occupational exposure constitutes a common risk factor for lung cancer. We observed molecular alterations in 73% of never-smokers, 35% of men and 8% of women were exposed to at least one occupational carcinogen. We report herein associations between molecular patterns and occupational exposure.BioCAST was a cohort study of lung cancer in never-smokers that reported risk factor exposure and molecular patterns. Occupational exposure was assessed via a validated 71-item questionnaire. Patients were categorised into groups that were unexposed and exposed to polycyclic aromatic hydrocarbons (PAH), asbestos, silica, diesel exhaust fumes (DEF), chrome and paints. Test results were recorded for EGFR, KRAS, HER2, BRAF and PIK3 mutations, and ALK alterations.Overall, 313 out of 384 patients included in BioCAST were analysed. Asbestos-exposed patients displayed a significantly lower rate of EGFR mutations (20% versus 44%, p=0.033), and a higher rate of HER2 mutations (18% versus 4%, p=0.084). ALK alterations were not associated with any occupational carcinogens. The DEF-exposed patients were diagnosed with a BRAF mutation in 25% of all cases. Chrome-exposed patients exhibited enhanced HER2 and PIK3 mutation frequency.Given its minimal effects in the subgroups, we conclude that occupational exposure slightly affects the molecular pattern of lung cancers in never-smokers. In particular, asbestos-exposed patients have a lower chance of EGFR mutations.
European Respiratory Journal

hal-01647080
https://hal-univ-rennes1.archives-ouvertes.fr/hal-01647080
DOI : 10.1183/13993003.00716-2017
PUBMED : 29074543

Éditeur(s) : HAL CCSD Nature Publishing Group
Résumé : International audience
Necroptosis is a programmed cell death pathway that has been shown to be of central pathophysiological relevance in multiple disorders (hepatitis, brain and cardiac ischemia, pancreatitis, viral infection and inflammatory diseases). Necroptosis is driven by two serine threonine kinases, RIPK1 (Receptor Interacting Protein Kinase 1) and RIPK3, and a pseudo-kinase MLKL (Mixed Lineage Kinase domain-Like) associated in a multi-protein complex called necrosome. In order to find new inhibitors for use in human therapy, a chemical library containing highly diverse chemical structures was screened using a cell-based assay. The compound 6E11, a natural product derivative, was characterized as a positive hit. Interestingly, this flavanone compound: inhibits necroptosis induced by death receptors ligands TNF-α (Tumor Necrosis Factor) or TRAIL (TNF-Related Apoptosis-Inducing Ligand); is an extremely selective inhibitor, among kinases, of human RIPK1 enzymatic activity with a nM Kd; has a non-ATP competitive mode of action and a novel putative binding site; is weakly cytotoxic towards human primary blood leukocytes or retinal pigment epithelial cells at effective concentrations; protects human aortic endothelial cells (HAEC) from cold hypoxia/reoxygenation injury more effectively than necrostatin-1 (Nec-1) and Nec-1s. Altogether, these data demonstrate that 6E11 is a novel potent small molecular inhibitor of RIPK1-driven necroptosis.
ISSN: 2045-2322

hal-01616456
https://hal-univ-rennes1.archives-ouvertes.fr/hal-01616456
https://hal-univ-rennes1.archives-ouvertes.fr/hal-01616456/document
https://hal-univ-rennes1.archives-ouvertes.fr/hal-01616456/file/s41598-017-12788-4.pdf
DOI : 10.1038/s41598-017-12788-4

Éditeur(s) : HAL CCSD
Résumé : International audience
Xth European Workshop Imagery Conference
St Andrews, United Kingdom

hal-01635689
https://hal.archives-ouvertes.fr/hal-01635689

Éditeur(s) : HAL CCSD
Résumé : National audience
Objective of the study An estimation of burden of serious fungal diseases in France is essential data to inform public health priorities on the importance of resources and research needed on these infections. In France, precise data are available for invasive fungal diseases but estimates for several other diseases such as chronic and immunoallergic diseases are by contrast less known. Materials and methods A systematic literature search was conducted using the Web of Science Platform. Published epidemiology papers reporting fungal infection rates from France were identified. Where no data existed, we used specific populations at risk and fungal infection frequencies in those populations to estimate national incidence or prevalence, depending on the condition. Results The model predicts high prevalences of severe asthma with fungal sensitization episodes (189 cases/100,000 adults per year), of allergic bronchopulmonary aspergillosis (145/100,000) and of chronic pulmonary aspergillosis (5.24/100,000). Besides, estimated incidence for invasive aspergillosis is 1.8/100,000 annually based on classical high risk factors. Estimates for invasive mucormycosis, pneumocystosis and cryptococcosis are 0.12/100,000, 1/100,000 and 0.2/100,000, respectively. Regarding invasive candidiasis, more than 10,000 cases per year are estimated, and a much higher number of recurrent vaginal candidiasis is probable but must be confirmed. Finally, this survey was an opportunity to report a first picture of the frequency of tinea capitis in France. Conclusion Using local and literature data of the incidence or prevalence of fungal infections, approximately 1,000,000 (1.47%) people in France are estimated to suffer from serious fungal infections each year. © 2016 Elsevier Masson SAS
Journal de Mycologie Medicale

hal-01467554
https://hal-univ-rennes1.archives-ouvertes.fr/hal-01467554
DOI : 10.1016/j.mycmed.2016.11.001
PUBMED : 27887809

Éditeur(s) : HAL CCSD Royal Society, The
Résumé : International audience
Many studies have focused on the impacts of climate change on biological assemblages, yet little is known about how climate interacts with other major anthropogenic influences on biodiversity, such as habitat disturbance. Using a unique global database of 1128 local ant assemblages , we examined whether climate mediates the effects of habitat disturbance on assemblage structure at a global scale. Species richness and evenness were associated positively with temperature, and negatively with disturbance. However, the interaction among temperature, precipitation and disturbance shaped species richness and evenness. The effect was manifested through a failure of species richness to increase substantially with temperature in transformed habitats at low precipitation. At low precipitation levels, evenness increased with temperature in undisturbed sites, peaked at medium temperatures in disturbed sites and remained low in transformed sites. In warmer climates with lower rainfall, the effects of increasing disturbance on species richness and evenness were akin to decreases in temperature of up to 98C. Anthropogenic disturbance and ongoing climate change may interact in complicated ways to shape the structure of assemblages, with hot, arid environments likely to be at greatest risk.
ISSN: 0962-8452

hal-01323255
https://hal.archives-ouvertes.fr/hal-01323255
DOI : 10.1098/rspb.2015.0418

Éditeur(s) : HAL CCSD
Résumé : National audience
Introduction Streptococcus pneumoniae est le microorganisme le plus fréquemment responsable de méningites bactériennes communautaires. Notre objectif était de décrire leur présentation, leur prise en charge et leur pronostic à partir des données de la Cohorte nationale Observationnelle des Méningites Bactériennes communautaires de l’AdulTe (COMBAT).
17es Journées Nationales d’Infectiologie
Lille, France

Droits : info:eu-repo/semantics/OpenAccess
inserm-01413456
http://www.hal.inserm.fr/inserm-01413456
http://www.hal.inserm.fr/inserm-01413456/document
http://www.hal.inserm.fr/inserm-01413456/file/2016%2C%20Tubiana%20-%20NEURO06.pdf
PUBMED : 27495605

Éditeur(s) : HAL CCSD
Résumé : International audience
High Pressure - Low Temperature (HP-LT) rocks testify for burying and exhumation of crustal material in subduction zones. The question of their exhumation is nevertheless still unclear. The Aegean domain offers several HP-LT belts. Especially, the Attic-Cycladic Blueschist unit (ACBU) is an example of a HP-LT unit exhumed in a convergent and then in a divergent context. It actually appears in metamorphic core complexes (MCCs) formed in the back-arc of the subduction. The syn-convergence "message" is however difficult to read because of the post-convergence structural and thermal overprint that accompanied the formation of the MCCs. Particularly, the geometry, the timing and the amount of the syn-orogenic exhumation remain unclear. We investigate theses questions with metamorphic petrology (Andros and Ios islands) and phengite population, single grain and in situ 40Ar-39Ar geochronology (Andros, Tinos, Syros and Ios islands). Based on these new constraints and a synthesis of published data, we are able to refine the geometry, the timing and the modes of the exhumation of the ACBU. Data from the whole Cyclades exhibit little differences in the age and P-T conditions at the successive stages of syn-orogenic exhumation. The peak of pressure (500±50 °C-18±1 kbar) has been reached at 50-55 Ma. The onset of the exhumation occurred at 40-45 Ma in blueschist facies conditions, between a basal thrust and a summital detachment. The following decompression was accompanied by cooling until 30-35 Ma (400±50 °C-8±1 kbar). At that time, the boundary conditions switched to extension. The change in boundary conditions is recorded by slowing of the exhumation and isobare heating. The conditions of post-orogenic exhumation differ from an island to another. The onset of post-orogenic exhumation is dated around 30 Ma in the northern Cyclades and 25 Ma in the southern Cyclades. It is related to top-to-the-North deformation in all the studied domain. The end of ductile deformation below the detachments is also diachronous (21 Ma in the North and 18 Ma in the South). Finally, we propose a model for the final steps of the exhumation related to the intrusion of granitic bodies around 14 Ma.
American Geophysical Union, Fall Meeting
San Francisco, United States

insu-00860510
https://hal-insu.archives-ouvertes.fr/insu-00860510
BIBCODE : 2010AGUFM.T23C2301L

Éditeur(s) : HAL CCSD American Society for Microbiology
Résumé : International audience
ISSN: 0066-4804

pasteur-00590987
https://hal-riip.archives-ouvertes.fr/pasteur-00590987
DOI : 10.1128/AAC.01532-06
PUBMED : 17438062

Éditeur(s) : HAL CCSD
Résumé : International audience
o abstract
Revue Des Maladies Respiratoires

hal-01647782
https://hal-univ-rennes1.archives-ouvertes.fr/hal-01647782
DOI : 10.1016/j.rmr.2017.07.017
PUBMED : 28939155

Éditeur(s) : HAL CCSD Taylor & Francis
Résumé : International audience
ISSN: 2001-3078

hal-01679029
https://hal.univ-antilles.fr/hal-01679029
DOI : 10.1080/20013078.2017.1305677

Éditeur(s) : HAL CCSD Springer Verlag (Germany)
Résumé : International audience
Tight control of basal cytosolic Ca(2+) concentration is essential for cell survival and to fine-tune Ca(2+)-dependent cell functions. A way to control this basal cytosolic Ca(2+) concentration is to regulate membrane Ca(2+) channels including store-operated Ca(2+) channels and secondary messenger-operated channels linked to G-protein-coupled or tyrosine kinase receptor activation. Orai, with or without its reticular STIM partner and Transient Receptor Potential (TRP) proteins, were considered to be the main Ca(2+) channels involved. It is well accepted that, in response to cell stimulation, opening of these Ca(2+) channels contributes to Ca(2+) entry and the transient increase in cytosolic Ca(2+) concentration involved in intracellular signaling. However, in various experimental conditions, Ca(2+) entry and/or Ca(2+) currents can be recorded at rest, without application of any experimental stimulation. This led to the proposition that some plasma membrane Ca(2+) channels are already open/activated in basal condition, contributing therefore to constitutive Ca(2+) entry. This article focuses on direct and indirect observations supporting constitutive activity of channels belonging to the Orai and TRP families and on the mechanisms underlying their basal/constitutive activities.
ISSN: 0175-7571

hal-01528591
https://hal.archives-ouvertes.fr/hal-01528591
DOI : 10.1007/s00249-017-1216-8
PUBMED : 28516266

Éditeur(s) : HAL CCSD
Résumé : International audience
Mylonite bodies are exhumed extensions at depth of shallow structural level brittle faults. They are characterised by intense strain localisation involving large volumes of middle to lower continental crust. The analysis of shear zone geometry and microstructures is a useful tool to decipher deformation kinematics, though finite strain estimation is still relatively qualitative. Timing of the deformation has also been the focus of numerous studies using the complete spectrum of geochronometers. However, only rare examples provide geochronological data that can be unambiguously interpreted and cooling, recrystallisation and neocrystallisation are difficult to tell from each other. Thus, time-scales over which major mylonite zones develop and remain active under ductile conditions as well as their strain rate often remain poorly documented. We investigated two sections across the South-Armorican Shear Zone (SASZ), a crustal-scale pluri-km thick dextral shear zone active during a single tectonic event of late-Variscan age and associated with syntectonic granite emplacement.. Finite strain profiles on these cross-sections, quantified from average Quartz grain-size in 34 samples, show a strong deformation partitioning towards the center of the SASZ, with a decrease, for each section, from 150µm in slightly deformed zones to less than 7-5 µm in the core of the ultramylonite. Furthermore, toward the core of the SASZ, development of a penetrative shear band network progressively eliminates lenses of less deformed rocks and results in homogenisation of the Quartz grain sizes. In parallel, we performed in-situ UV-laser Ar/Ar dating on the fabric-forming white micas. White micas population can be decomposed into inherited muscovites carried by the folation and newly-formed, syn-tectonic substituted phengites located along the shear bands yielding 2 age groups for each sample. Along the profiles, the difference between these two ages evolves from 10-15 Ma in weakly deformed domains to zero toward the SASZ core. In addition, mylonite-forming phengites yielded the same age irrespectively of their position on the section or of the rock strain.. On the regional point of view, this study brings deformation-ages for the late-orogenic evolution of the South Armorican domain. Strain accumulated along the SASZ in a time-span extending to 300-298 Ma, i.e. contemporaneously with the activity of nearby major extensional shear zones. These results support a new model of crustal-scale shear zone activity, where the whole body of the shear zone is active throughout its history (defined as the largest age difference through the section), i.e. the actively deforming zone does not get progressively narrower. The strain distribution nevertheless evolves with time, with an increasingly large fraction partitioned in the ultramylonitic core.
EGU General Assembly
Vienne, Austria

insu-00839320
https://hal-insu.archives-ouvertes.fr/insu-00839320
BIBCODE : 2010EGUGA..12.9881A

Éditeur(s) : HAL CCSD
Résumé : International audience
Amazonie brésilienne, usages et représentations du territoire

ISBN : 102371540358
hal-01678070
https://hal.archives-ouvertes.fr/hal-01678070

Éditeur(s) : HAL CCSD
Résumé : International audience
Background Orodental diseases include several clinically and genetically heterogeneous disorders that can present in isolation or as part of a genetic syndrome. Due to the vast number of genes implicated in these disorders, establishing a molecular diagnosis can be challenging. We aimed to develop a targeted next-generation sequencing (NGS) assay to diagnose mutations and potentially identify novel genes mutated in this group of disorders. Methods We designed an NGS gene panel that targets 585 known and candidate genes in orodental disease. We screened a cohort of 101 unrelated patients without a molecular diagnosis referred to the Reference Centre for Oro-Dental Manifestations of Rare Diseases, Strasbourg, France, for a variety of orodental disorders including isolated and syndromic amelogenesis imperfecta (AI), isolated and syndromic selective tooth agenesis (STHAG), isolated and syndromic dentinogenesis imperfecta, isolated dentin dysplasia, otodental dysplasia and primary failure of tooth eruption. Results We discovered 21 novel pathogenic variants and identified the causative mutation in 39 unrelated patients in known genes (overall diagnostic rate: 39%). Among the largest subcohorts of patients with isolated AI (50 unrelated patients) and isolated STHAG (21 unrelated patients), we had a definitive diagnosis in 14 (27%) and 15 cases (71%), respectively. Surprisingly, COL17A1 mutations accounted for the majority of autosomal-dominant AI cases. Conclusions We have developed a novel targeted NGS assay for the efficient molecular diagnosis of a wide variety of orodental diseases. Furthermore, our panel will contribute to better understanding the contribution of these genes to orodental disease. Trial registration numbers NCT01746121 and NCT02397824
Journal of Medical Genetics

hal-01272929
https://hal-univ-rennes1.archives-ouvertes.fr/hal-01272929
DOI : 10.1136/jmedgenet-2015-103302
PUBMED : 26502894

Éditeur(s) : HAL CCSD
Résumé : International audience
INTRODUCTION: The syndrome of combined pulmonary fibrosis and emphysema (CPFE) is characterized by imaging features consisting of the association of centrilobular and/or paraseptal emphysema and pulmonary fibrosis. Virtually all patients are smokers and thus at high risk of developing lung cancer. METHODS: This retrospective multicentre study was conducted by the Groupe d'Etudes et de Recherche sur les Maladies "Orphelines" Pulmonaires (GERM"O"P). RESULTS: A total of 47 patients presenting with lung cancer and CPFE syndrome were identified. All patients were smokers, with a mean of 47 pack-years. A pathological diagnosis of lung cancer was obtained for 38 (81%) patients. Histological type was squamous cell carcinoma in 17 (36%) patients, adenocarcinoma in 14 (30%), non-small-cell lung cancer not otherwise specified in three (6%), small-cell lung cancer in three (6%), and sarcomatoid carcinoma in one (2%). Overall, 20 of the 47 patients could not receive standard-of-care treatment for lung cancer, as per international recommendations or guidelines; this limitation was considered to be directly related to the CPFE syndrome in eight (40%) cases. CONCLUSION: Lung cancer in patients with CPFE syndrome represents a specific entity with a poor prognosis, that further represents the most characteristic and severe model of tobacco-related disease.
Journal of Thoracic Oncology: Official Publication of the International Association for the Study of Lung Cancer

hal-01063912
https://hal.archives-ouvertes.fr/hal-01063912
DOI : 10.1097/JTO.0000000000000209
PUBMED : 25157769