Résumé : Herbácea, suculenta, aromática, generalmente de menos de 1 m. Hojas ovadas, cuneadas u obtusas en el ápice, subcordadas en la base, márgenes crenados, pubescentes. Flores en racimos de 10 a 30 cm; cáliz tomentoso, labio superior oblongo; corola blanquecina, rosada o lila.
Herbacée succulente, aromatique, atteignant généralement moins de 1 m. Feuilles ovées, avec pointe obtuse, base subcordée, bords crénelés, pubescents. Fleurs en grappes de 10 à 30 cm; calice tomenteux, lèvre supérieure oblongue; corolle blanchâtre, rosée ou couleur lilas.
Perennial herb, 60-100 cm high,with soft thick-stems, succulent, hairy, aromatic.  Leaves blades, 2.5-4.5 x 2.5-4 cm, thick and soft, broadly ovate, truncate-subcordate at the base, apex obtuse to rounded, margins crenate, both surfaces densely pubescent with glands; inflorescence a raceme 10-30 cm long; flowers bilabiate, corolla 7-9 mm long, lilac to whitish.
Nativa de Asia tropical y Africa, cultivada en las zonas tropicales del mundo.
Originaire de l’;Asie tropicale et d’;Afrique, cultivée dans les zones tropicales du monde.
Native to tropical Asia and Africa, cultivated in tropical regions

http://www.tramil.net/fototeca/plant287

Éditeur(s) : HAL CCSD BioMed Central
Résumé : International audience
Background : Trichoderma reesei is the main industrial source of cellulases and hemicellulases required for the hydrolysis of biomass to simple sugars, which can then be used in the production of biofuels and biorefineries. The highly productive strains in use today were generated by classical mutagenesis. As byproducts of this procedure, mutants were generated that turned out to be unable to produce cellulases. In order to identify the mutations responsible for this inability, we sequenced the genome of one of these strains, QM9136, and compared it to that of its progenitor T. reesei QM6a.Results : In QM9136, we detected a surprisingly low number of mutagenic events in the promoter and coding regions of genes, i.e. only eight indels and six single nucleotide variants. One of these indels led to a frame-shift in the Zn2Cys6 transcription factor XYR1, the general regulator of cellulase and xylanase expression, and resulted in its C-terminal truncation by 140 amino acids. Retransformation of strain QM9136 with the wild-type xyr1 allele fully recovered the ability to produce cellulases, and is thus the reason for the cellulase-negative phenotype. Introduction of an engineered xyr1 allele containing the truncating point mutation into the moderate producer T. reesei QM9414 rendered this strain also cellulase-negative. The correspondingly truncated XYR1 protein was still able to enter the nucleus, but failed to be expressed over the basal constitutive level.Conclusion : The missing 140 C-terminal amino acids of XYR1 are therefore responsible for its previously observed auto-regulation which is essential for cellulases to be expressed. Our data present a working example of the use of genome sequencing leading to a functional explanation of the QM9136 cellulase-negative phenotype.
ISSN: 1471-2164

Droits : http://creativecommons.org/licenses/by/
hal-01149671
http://hal.upmc.fr/hal-01149671
http://hal.upmc.fr/hal-01149671/document
http://hal.upmc.fr/hal-01149671/file/Lichius_2015_Genome_sequencing.pdf
DOI : 10.1186/s12864-015-1526-0

Éditeur(s) : HAL CCSD Geological Society of America
Résumé : International audience
Integrated Ocean Drilling Program Expedition 313 continuously cored uppermost Eocene to Miocene sequences on the New Jersey shallow shelf (Sites M27, M28, and M29). Previously, 15 Miocene (ca. 23-13 Ma) seismic sequence boundaries were recognized on several generations of multichannel seismic profi les using criteria of onlap, downlap, erosional truncation, and toplap. We independently recognize sequence boundaries in the cores and logs based on an integrated study of core surfaces, lithostratigraphy and process sedimentology (grain size, mineralogy, facies, and paleoenvironments), facies successions, stacking patterns, benthic foraminiferal water depths, downhole logs, core gamma logs, and chronostratigraphic ages. We use a velocitydepth function to predict the depths of seismic sequence boundaries that were tested by comparison with major core surfaces, downhole and core logs, and synthetic seismograms. Using sonic velocity (core and downhole), core density, and synthetic seismograms, we show that sequence boundaries correspond with acoustic impedance contrasts, although other stratal surfaces (e.g., maximum fl ooding and transgressive surfaces) also produce refl ections. Core data are suffi cient to link seismic sequence boundaries to impedance contrasts in 9 of 12 instances at Site M27, 6 of 11 instances at Site M28, and 8 of 14 instances at Site M29. Oligocene sequences have minimal lithologic and seismic expression due to deep-water locations on clinoform bottomsets. Miocene sequences (ca. 23-13 Ma) were sampled across several unconformity clinothems (prograding units) on topset, foreset, and bottomset locations. Excellent recovery allows core-seismic integration that confi rms the hypothesis that unconformities are a primary source of impedance contrasts. Our core-seismic-log correlations predict that key seismic surfaces observed in other sub surface investigations without core and/or well logs are stratal surfaces with sequence stratigraphic signifi cance.
EISSN: 1553-040X

insu-00946235
https://hal-insu.archives-ouvertes.fr/insu-00946235
DOI : 10.1130/GES00858.1

Éditeur(s) : HAL CCSD Elsevier
Résumé : International audience
On the Gulf of Lions margin, the Messinian Salinity Crisis (MSC) event led to a significant and widespread erosion of the Miocene shelf and slope. It is thus difficult nowadays to restore the morphology of the margin at the beginning of the crisis, and to study the Middle-Upper Miocene series rarely preserved from erosion. In this study, thanks to the use of multichannel seismic reflection data from the Gulf of Lions western slope, it has been possible to image the Miocene slope deposits. Interpretation of the data reveals several diachronic sediment bodies just beneath the Miocene/Pliocene boundary. These bodies are related to slope erosional-depositional complexes, underlain by erosional unconformities outlined by seismic reflector truncations. They are V- or U-shaped in cross section. Over their mapped extent, these bodies are typically 8-12 km wide and incise up to 600 ms TWTT into the Miocene slope deposits. in plan view, they are roughly elongated NW-SE, perpendicularly to the present day shelf break, and they progressively migrate towards the South-East. We interpret these seismic bodies as buried Miocene submarine canyons pre-dating the dramatic drawdown related to the MSC. In this study we try to constrain their age, and we discuss the events that may be at their origin. We also discuss the long-term impact of these slope canyons on the evolution of the margin.
ISSN: 0264-8172

hal-00411546
https://hal.archives-ouvertes.fr/hal-00411546
DOI : 10.1016/j.marpetgeo.2008.04.006

Éditeur(s) : HAL CCSD Elsevier
Résumé : International audience
The Chenaillet Ophiolite in the Franco-Italian Alps represents a well-exposed ocean-floor sequence that was only weakly overprinted by Alpine metamorphism during its emplacement in the Alpine nappe stack. Pillow lavas showing a Mid Ocean Ridge Basalt (MORB) signature overlie tectonically exhumed gabbros and serpentinized mantle rocks similar to oceanic core complexes (OCC) described from present-day slow- to ultraslow-spreading ridges. Based on detailed mapping it can be shown that: 1) syn-magmatic high-temperature shear zones are truncated by oceanic detachment faults; 2) sediments consisting exclusively of clasts derived from the footwall of the detachment are overlain by voluminous lavas, and 3) emplacement of lavas is linked with high-angle faulting that overprint the oceanic detachment fault. Based on stratigraphic and cross cutting relationships we conclude that: 1) a complex relation between high- and low-temperature shear zones exists, 2) oceanic detachment faults may be obliterated by later emplaced of MOR basalts, and 3) high-angle faults may serve at shallow levels as feeder channels for the overlying pillow basalts. These observations raise questions about the relative relations in time and space between magmatic, hydrothermal and tectonic processes at spreading systems. We propose that the observed change from exhumation along an oceanic detachment fault to high-angle faulting associated with extrusion of volcanic rocks may represent an evolution within a life cycle of a spreading system that may be comparable to present-day slow- to ultraslow-spreading ridges.
ISSN: 0024-4937

hal-00617678
https://hal.archives-ouvertes.fr/hal-00617678
DOI : 10.1016/j.lithos.2010.10.017

Éditeur(s) : HAL CCSD
Résumé : International audience
In this paper, via the algebra of generalized functions, we investigate the generalized Riemann's problem associated to conservation laws with analytical coefficients. This allows us to transform the problem into a system of ordinary differential equations. In some particular cases, such that Burgers' and conservative Richard's equation, approximated solutions are obtained by the truncation of the so called Hugoniot-Maslov's chain and numerical simulations are also presented in the case of equations with polynomial coefficients.
Novi Sad Journal of Mathematics

hal-00771405
https://hal.archives-ouvertes.fr/hal-00771405

Éditeur(s) : Elsevier
Résumé : Clinoforms on modern shelves and slopes, as well as in ancient rock records, are widely recognized as a fundamental building element of continental margin growth. Regardless of their dominant lithology, clinoforms are composed of three geometric elements: topset, foreset and bottomset. Traditionally, much emphasis in the study of clinoforms was put on the geometry of the topset, viewed as the most energetic portion of a clinoform and studied to discern if aggradation was active rather than erosional truncation, and on the foreset, the area with the highest sediment accumulation rates. Here we focus on the factors forcing clinoforms to taper out and on the inferred mechanisms for bottomset creation. We base our analysis on muddy shelf clinoforms, a particular class of clinoforms that is typical of low-gradient settings and is characterized by a substantial component of shore-parallel sediment transport. This paper is based on a large dataset of CHIRP-sonar profiles, bathymetric and isopach maps of the Late Holocene clinoform on the Adriatic shelf, where integrated stratigraphic studies from sediment cores allow a very high (in some cases century-scale) chronological resolution. Knowledge on the dominant oceanographic regime affecting sediment dispersal and, ultimately, clinoform development, has been recently refined during the EuroSTRATAFORM collaboration. Muddy clinoforms are markedly three-dimensional features that cannot be entirely understood solely on two-dimensional shore normal sections. We suggest that this class of clinoform is advection dominated and that an increase in the energy regime at the toe of the foreset may prevent sediment from reaching beyond the transition to the bottomset region.
Continental Shelf Research (0278-4343) (Elsevier), 2007-02 , Vol. 27 , N. 3-4 , P. 506-525

Droits : 2006 Elsevier Ltd All rights reserved
http://archimer.ifremer.fr/doc/2007/publication-2409.pdf
DOI:10.1016/j.csr.2006.11.013
http://archimer.ifremer.fr/doc/00000/2409/

Éditeur(s) : HAL CCSD Nature Publishing Group
Résumé : International audience
CYLD is a tumour suppressor gene mutated in familial cylindromatosis, a genetic disorder leading to the development of skin appendage tumours. It encodes a deubiquitinating enzyme that removes Lys63- or linear-linked ubiquitin chains. CYLD was shown to regulate cell proliferation, cell survival and inflammatory responses, through various signalling pathways. Here we show that CYLD localizes at centrosomes and basal bodies via interaction with the centrosomal protein CAP350 and demonstrate that CYLD must be both at the centrosome and catalytically active to promote ciliogenesis independently of NF-κB. In transgenic mice engineered to mimic the smallest truncation found in cylindromatosis patients, CYLD interaction with CAP350 is lost disrupting CYLD centrosome localization, which results in cilia formation defects due to impairment of basal body migration and docking. These results point to an undiscovered regulation of ciliogenesis by Lys63 ubiquitination and provide new perspectives regarding CYLD function that should be considered in the context of cylindromatosis.
ISSN: 2041-1723

hal-01063915
https://hal.archives-ouvertes.fr/hal-01063915
DOI : 10.1038/ncomms5585
PUBMED : 25134987

Éditeur(s) : HAL CCSD American Chemical Society
Résumé : The distribution of water and ions in nanoporous membranes, in particular close to their surfaces with external reservoirs, is investigated by means of molecular dynamics (MD) simulations using classical polarizable force fields. A sodium iodide (NaI) aqueous solution is considered in uncharged weakly hydrophilic (WH) and highly hydrophilic (HH) nanoporous materials modeled by a single truncated nanopore in contact with two reservoirs. In the inner part of the nanopore, the spatial distribution of water and ions is obtained by coupling semi-infinitesimal longitudinal and semi-infinitesimal radial descriptions. In the HH material, in contrast with the WH material, species are no longer uniformly distributed in the inner nanopore and water molecules are distributed in a nearly frozen axially periodic corona-like structure which leads to the formation of ionic tunnels. In the reservoirs, water molecules and ions attracted by the membrane surface create a necking of the confined solution which acts as a barrier at the entrance of the nanopore. In the WH nanopore subsurface, close to the surface of the membrane, the species distribution is analogous to the interface distribution between an aqueous electrolyte solution and air. In the HH nanopore subsurface, this distribution is modulated by the water corona-like structure. These results provide useful information for predicting properties of nanoporous membranes, in particular the drastic reduction of diffusion coefficients in HH materials, and give a guide to designing synthetic membranes for applications in nanofiltration, etc.
ISSN: 1932-7447

hal-00682155
https://hal.archives-ouvertes.fr/hal-00682155
DOI : 10.1021/jp2078924

Éditeur(s) : HAL CCSD
Résumé : This PhD work is based on field, petrographic, and geochemical observations of rocks originated at the base of the sheeted dike complex, in the Oman ophiolite and at IODP Site 1256, coupled with an experimental study. It provides new constrains on processes that occur at the magma / hydrothermal system transition in oceanic crust formed at fast spreading ridges. The base of the sheeted dike complex is truncated by intrusive isotropic gabbros, and therefore reheated and recrystallized to the "granoblastic dikes" under temperatures up to 1030°C. Xenoliths of granoblastic microgabbros and microgabbronorites derived from the granoblastic dikes are commonly observed in the about 100 meters thick horizon of isotropic gabbro that underlies the sheeted dike complex. These features can be explained by upward migrations of the melt lens that is present at fast spreading centers. The occurrence of several assimilation features (xenoliths and granoblastic patches) in the isotropic gabbro horizon supports the hypothesis that this horizon represents the fossilization of the upper melt lens. The experimental study was designed to simulate experimentally the effect of partial melting of hydrothermally altered sheeted dikes. The results show that melting starts at 850°C, confirm the residual origin of granoblastic dikes and xenoliths, and attest to the anatectic origin of the oceanic plagiogranites that are commonly present close to the base of the sheeted dike complex. The major and trace element composition of the experimental anatectic melt that represents the main contaminant for primitive MORBs at fast spreading ridges has been determined. The upper axial melt lens at fast spreading mid-ocean ridges is herein described as a dynamic system that can migrate vertically, and which fossilizes when moving off-axis.
Ce travail de thèse est basé sur des observations de terrain, sur une étude pétrographique et géochimique des roches formées à la base du complexe filonien dans l'ophiolite d'Oman et au niveau du Site IODP 1256, ainsi que sur une étude expérimentale. De nouvelles contraintes sont apportées sur les processus se produisant à la transition magma / système hydrothermal dans la croute océanique formée au niveau des dorsales à expansion rapide. L'intrusion de gabbros isotropes dans la base du complexe filonien a provoqué son réchauffement et sa recrystallization en « dikes granoblastiques » jusqu'à des températures de 1030°C. Des xénolites de microgabbro à orthopyroxene dérivées des dikes granoblastiques sont souvent observées dans le niveau de gabbros isotropes épais de 100 mètres environ qui est présent à la base du complexe filonien. Ces différentes caractéristiques sont à relier à des migrations verticales vers le haut du sommet de la lentille magmatique supérieure qui est observée aux dorsales rapides. Les nombreuses évidences d'assimilation (xénolites et patchs granoblastiques) dans le niveau des gabbros isotropes appuient l'hypothèse que ce niveau représente la fossilisation de la lentille magmatique supérieure. L'étude expérimentale a consisté à tester l'effet de la fusion partielle du complexe filonien préalablement hydrothermalisé. Les résultats montrent que la fusion commence à 850°C, confirment l'origine résiduelle des dikes granoblastiques et des xénolites associées, et attestent de l'origine anatectique des plagiogranites océaniques qui sont couramment observés à proximité de la base du complexe filonien. La composition en éléments majeurs et traces du liquide anatectique a été déterminée. Ce liquide représente le principal contaminant pour les MORBs primitifs émis au niveau des dorsales rapides. La lentille magmatique supérieure présente au niveau des dorsales médio-océaniques à expansion rapide est ici décrite comme un système dynamique qui peut migrer verticalement, et qui est fossilisée lorsqu'elle se déplace hors axe.
https://tel.archives-ouvertes.fr/tel-00448699

tel-00448699
https://tel.archives-ouvertes.fr/tel-00448699
https://tel.archives-ouvertes.fr/tel-00448699/document
https://tel.archives-ouvertes.fr/tel-00448699/file/France_2009_PhD.pdf

Éditeur(s) : HAL CCSD Springer Verlag (Germany)
Résumé : International audience
A hybrid linear ion-trap Fourier-transform ion cyclotron resonance mass spectrometer was used for top-down characterization of the abundant human salivary Cystatins, including S, S1, S2, SA, SN, C, and D, using collisionally activated dissociation (CAD) after chromatographic purification of the native, disulfide intact proteins from saliva. Post-translational modifications and protein sequence polymorphisms arising from single nucleotide polymorphisms (SNPs) were assigned from precursor and product ion masses at a tolerance of 10 ppm allowing confident identification of individual intact mass tags. Cystatins S, S1, S2, SA and SN were cleaved of a N-terminal 20 amino-acid signal peptide, and Cystatin C a 26-residue peptide, to yield a generally conserved N-terminus. In contrast, Cystatin D isoforms with 24 and 28 amino-acid residue N-terminal truncations were found such that their N-termini were not conserved. Cystatin S1 was phosphorylated at Ser3, while S2 was phosphorylated at Ser1 and Ser3 of the mature protein, in agreement with previous work. Both Cystatin D isoforms carried the polymorphism C46R (SNP: rs1799841). The 14328 Da isoform of Cystatin SN previously assigned with polymorphism P31L due to a SNP (rs2070856) was found only in whole saliva. Parotid secretions contained no detectable Cystatins while whole saliva largely mirrored the contents of submandibular/sublingual (SMSL) secretions. Top-down high-resolution mass spectrometry is a powerful tool for the identification and characterization of potential protein biomarkers in saliva.
ISSN: 1044-0305

hal-00677483
https://hal.archives-ouvertes.fr/hal-00677483
DOI : 10.1016/j.jasms.2010.01.025

Éditeur(s) : HAL CCSD Elsevier
Résumé : International audience
The Miradouro da Lua cliffs, which are 60 km south of Luanda, record the building and uplift of the late Neogene Palaeo-Cuanza delta. The detailed study of the sedimentary architecture and stacking pattern permitted separation of the pile into five depositional units bounded by erosional surfaces and characterised by separate facies associations (genetic sequences = units in this paper). At the base of the series, aeolian deposits (Unit 1) mark the development of a possible coastal desert during the late Miocene aridification. The major Pliocene sea-level rise (Transgressive Systems Tract) led to the drowning of the continental platform into a discrete shoreface-foreshore sequence (Unit 2), followed by an expanded deltaic sequence (Unit 3) that represents the main outcrop of the area. The sedimentary fabric of this prograding wedge during the Highstand Systems Tract reveals laterally stacked pluri-hectometic mouth bars built by the abrupt switching of a bird-foot delta during the Pliocene highstand. The clinoforms are deeply incised by submarine gullies filled both by periodic river-driven turbidite and tidal currents (Unit 4) during the coeval growing of the delta. The topset of the prograding wedge and associated gullies infill is truncated by an overall erosional unconformity that marks the widespread development of an extensive braid-delta system (Unit 5) during the lower Pleistocene sea-level drop (Lowstand Systems Tract). The last 6 m of the Braid-delta unit is overprinted by a ferallitic profile, forming the surface of the plateau and indicating long-term subaerial exposure and weathering processes consistent with the maximum warming of the middle to late Pleistocene interglacial periods. The successive abrupt shifts of the depositional systems through the sedimentary pile indicate a high-amplitude sea level amplified by major coastal uplifts and the reorganisation of the fluvial network. In this context, the palaeo-Cuanza prograding wedge signals the westward tilting and 60 m total uplift of the coastal plain during the Pliocene. A new uplift pulse by differential tilt and slope increase is deduced from the widespread development of the braid-delta complex during the early Pleistocene. The last tectonic event corresponds to the 80- to 100-m uplift and ferallitic paedogenesis during the middle to late Pleistocene interglacial stages.
ISSN: 0899-5362

hal-01053716
https://hal.archives-ouvertes.fr/hal-01053716
DOI : 10.1016/j.jafrearsci.2014.01.013

Éditeur(s) : HAL CCSD Public Library of Science
Résumé : International audience
Many studies have reported proliferative, differentiating or protective effects of estradiol, notably through estrogen receptor alpha (ERα). On the contrary, the ligand-independent action of ERα is currently poorly documented notably in cell protection. The stable transfection of wild type, substituted or truncated form of ERα in PC12 cells (ERα negative cell line) lead the specific study of its ligand-independent action. Hence, we demonstrate here that, in the absence of E2, the expression of ERα prevents cells from apoptosis induced by serum deprivation. This protection is not due to an ERE-mediated transcription and does not require either AF-1 or AF-2 transactivation functions. It is afforded to the Y537 residue of ERα and activation of c-Src/Stat3 signaling pathway.
ISSN: 1932-6203

inserm-00865332
http://www.hal.inserm.fr/inserm-00865332
http://www.hal.inserm.fr/inserm-00865332/document
http://www.hal.inserm.fr/inserm-00865332/file/PMID_23825704.pdf
DOI : 10.1371/journal.pone.0069081

Éditeur(s) : Gauthier-Villars
Résumé : Seismic data from the deep Gulf of Mexico basin indicate: 1) inferred ocenaic crust in the deep central Gulf (5 to 6 km thick; 6.8 to 7.1 km/sec.) is flanked symmetrically on the north and south by inferred transitional crust (8 to 15 km thick; 6.4 to 6.8 km/sec.); 2) acoustic basement seen on the reflection data in the central Gulf is an irregular reflector and probably represents the top of an oceanic volcanic (basaltic) layer (layer 2); 3) northof the Campeche Escar pment the top of transitional crust is represented by a strong, smooth reflector/unconformity that truncates rift basins and is onlapped by a thick salt and sedimentary section; 4) in the southeastern Gulf transitional crust consists of tilted basement blocks probably representing a thinned and rifted continental crust; lows between the blocks are filled with synrift sediments; 5) thick salt symmetrically flanks the north and south sides of the oceanic crust; 6) seismic stratigraphic analysis suggests that early sediments in the Gulf in areas of transitional crust represent an upward gradation from volcanics and nonmarine sediments including evaporites to shallow marine and then to deep marine; only deep marine sediments occur in the central Gulf overlying oceanic crust.
Oceanologica Acta, Special issue (0399-1784) (Gauthier-Villars), 1981

Droits : info:eu-repo/semantics/openAccess
http://archimer.ifremer.fr/doc/00246/35679/34187.pdf
http://archimer.ifremer.fr/doc/00246/35679/

Éditeur(s) : HAL CCSD Elsevier
Résumé : International audience
We study diffusion in a heterogeneous medium that is characterized by spatially varying diffusion properties from a random walk point of view. We show that an inhomogeneous continuous time random walk (CTRW) with a spatially variable exponential transition time distribution solves the spatially discretized heterogeneous diffusion equation. This demonstrates the equivalence of the widely used time-domain random walk (TDRW) scheme and spatially inhomogeneous CTRW and at the same time provides a demonstration of the formal equivalence of the TDRW particle formulation and the heterogeneous diffusion equation. Based on this equivalence, we develop a TDRW method for heterogeneous diffusion under spatially variable multirate mass transfer properties. We discuss the implementation of these schemes and study the diffusion behavior in the presence of traps that are characterized by a truncated power-law trapping time distribution.
ISSN: 0309-1708

hal-00767566
https://hal.archives-ouvertes.fr/hal-00767566
DOI : 10.1016/j.advwatres.2012.07.015

Éditeur(s) : HAL CCSD Royal Society
Résumé : International audience
Maternal Embryonic Leucine zipper Kinase (MELK) was recently shown to be involved in cell division of Xenopus embryo epithelial cells. The cytokinetic furrow of these cells ingresses asymmetrically and is developmentally regulated. Two subpopulations of xMELK, the mMELK (for "mitotic" xMELK) and iMELK ("interphase" xMELK), which differ in their spatial and temporal regulation, are detected in Xenopus embryo. How cells regulate these two xMELK populations is unknown. In this study we show that, in epithelial cells, xMELK is present at a higher concentration at the apical junctional complex, in contrast to mesenchyme-like cells, which have uniform distribution of cortical MELK. Interestingly, mMELK and iMELK also differ by their requirements towards cell-cell contacts to establish their proper cortical localization both in epithelial and mesenchyme-like cells. Receptor for Activated protein Kinase C (RACK1), which we identified as an xMELK partner, co-localizes with xMELK at the tight junction. Moreover, a truncated RACK1 construct interferes with iMELK localization at cell-cell contacts. Collectively, our results suggest that iMELK and RACK1 are present in the same complex and that RACK1 is involved in the specific recruitment of iMELK at the apical junctional complex in epithelial cells of Xenopus embryos.
ISSN: 2046-6390

hal-00874053
https://hal.archives-ouvertes.fr/hal-00874053
https://hal.archives-ouvertes.fr/hal-00874053/document
https://hal.archives-ouvertes.fr/hal-00874053/file/Cell-cycle_dependent-accepted.pdf
DOI : 10.1242/bio.20136080

Éditeur(s) : HAL CCSD Wiley
Résumé : International audience
We describe the simulation of an exterior problem using a magnetic field deriving from magnetostatics, with a numerical method mixing the approaches of C. I. Goldstein and L.-A. Ying. This method is based on the use of a graded mesh obtained by gluing homothetic layers in the exterior domain. On this mesh, we use an edge elements discretization and a recently proposed mixed formulation. In this paper, we provide both a theoretical and numerical study of the method. We establish an error estimate, describe the implementation, propose some preconditioning techniques and show the numerical results. We also compare these results with those obtained from an equivalent boundary elements approach. In this way, we retain that our method leads to a practical numerical implementation, a saving of storage, and turns out to be an alternative to the classical boundary elements method.
ISSN: 0749-159X

hal-00730196
https://hal.archives-ouvertes.fr/hal-00730196
DOI : 10.1002/num.10064