Éditeur(s) : HAL CCSD American Physiological Society
Résumé : Full text: http://jn.physiology.org/content/88/3/1475
International audience
The contribution of voltage-gated calcium channels (VGCC) to the development of cultured embryonic cockroach brain neurons was assessed using pharmacological agents. VGCC currents were recorded using the patch-clamp technique and were found to be blocked dose-dependently by micromolar concentrations of mibefradil. The activation and inactivation properties of the calcium channels enable a sizeable calcium current to flow at rest (about -30 and -20 mV in high-potassium culture media). As expected, the cytoplasmic-free calcium concentration was found to rise when the extracellular potassium concentration was raised from 3 to 15 and 30 mM. The effects of VGCC blockers and calcium chelators were different in fresh and in mature cultures in which the neurons were connected to each other to form a defined network. In fresh cultures, the two non-selective VGCC blockers (verapamil and mibefradil) induced a dose-dependent cell death that was proportional to their blocking effect on I(Ba). This effect could not be prevented by addition of fetal calf serum to the culture medium. A similar effect was obtained using intra- or extracellular calcium chelating agents (10 microM BAPTA-AM or 10 mM EGTA). Quite unexpectedly, blockade of the P/Q-like (omega-Aga WA-sensitive) component of the calcium current by 500 nM of omega-AgaTx IVA had no lethal effect, suggesting that the corresponding channels are not involved in the survival mechanism. As expected from their lack of effect on I(Ba), isradipine, nifedipine, and omega-CgTx GVIA did not induce cell death. When the neurons started growing neurites, their sensitivity to calcium channel blockade by mibefradil decreased, indicating a correlation between neurite outgrowth and resistance to calcium depletion. In mature cultures, the neurons became resistant to mibefradil, verapamil, and BAPTA-AM. However, these agents, as well as omega-AgaTx IVA, had a significant inhibitory effect on the increase in diameter of the connectives that linked adjacent clusters of neurons. This effect has been shown to result, in the case of mibefradil, from an inhibition of neurite outgrowth characterized by a significant reduction of the number of primary neurites and secondary branchings but not to a significant modification of the diameter of individual neurites. These results support the view that, as in vertebrates, calcium influx through VGCC plays an important role in survival and neurite outgrowth of cultured embryonic insect neurons. The differential contribution of the P/Q-like and R-like (omega-Aga WA-sensitive) calcium channels in these processes is discussed.
ISSN: 0022-3077

hal-00982287
https://hal.archives-ouvertes.fr/hal-00982287
DOI : 10.1152/jn.00749.2001
PUBMED : 12205168

Éditeur(s) : HAL CCSD Elsevier
Résumé : International audience
The seismic (elastic) properties of the crust have been modelled using hypothetical polycrystals with typical crustal compositions and commonly measured petrofabrics. This modelling allows us to better evaluate and quantify the crustal contribution to splitting of teleseismic SKS-waves. Mafic rocks such as anorthosite or pyroxene-bearing gabbros have complex S-waves properties, i.e. with small shear-wave anisotropies (< 3%) and without clear relationships between the fast shear-wave polarization plane orientations and the fabric. On the other hand foliated felsic rocks such as gneisses or schists and mafic rocks such as amphibole-bearing gabbros are strongly birefringent (anisotropies greater than 5%) and exhibit clear relationships with the structure. Biotite and amphibole preferred orientations clearly play a major rote in shear-wave splitting in crustal rocks. We show strong correlations between the orientation of the foliation and the amplitude of the delay time. Maximum delay time is systematically observed for waves propagating parallel to the foliation. The fast shear-wave is furthermore polarized parallel to the foliation plane. We calculate a delay time of about 0.1-0.2 s per 10 km of crustal rocks.
ISSN: 0031-9201

hal-01389713
http://hal.univ-reunion.fr/hal-01389713
http://hal.univ-reunion.fr/hal-01389713/document
http://hal.univ-reunion.fr/hal-01389713/file/Barruol_SKS_crust_PEPI1993_hal.pdf
DOI : 10.1016/0031-9201(93)90161-2

Éditeur(s) : HAL CCSD Elsevier
Résumé : International audience
The Environmental Relative Moldiness Index (ERMI) is a DNA-based metric developed to describe the fungal contamination in US dwellings. Our goal was to determine if the ERMI values in dwellings in north western France were correlated with other measures of fungal contamination. Dust and air samples were obtained from 40 dwellings and analyzed by quantitative PCR and/or by culturing. These dwellings were also inspected and the amount of visible fungal growth estimated in m(2). The ERMI values in these dwellings ranged from -2.7 to 28.8 and the fungal contamination estimates ranged from 0 to 20 m(2). The 40 dwellings were divided into those with a low (<6) or high (>8) ERMI values (n=20 in each). The average ERMI value was 15.70 for the high ERMI compared to 2.68 for the low ERMI dwellings. ERMI values were correlated (significant Kendall's tau values) with concentrations of fungi in air samples analyzed by QPCR or culturing. ERMI values were also correlated (significant Kendall's tau values) with the visible estimates of fungal contamination. Older dwellings were more likely to have higher ERMI values. These results suggest that the ERMI dust sample, which is quick and easy to collect, may be useful in making decisions about reducing fungal exposures in homes.
ISSN: 0048-9697

hal-00874733
https://hal.archives-ouvertes.fr/hal-00874733
DOI : 10.1016/j.scitotenv.2012.08.085
PUBMED : 23022719

Éditeur(s) : HAL CCSD Société Géologique de France
Résumé : International audience
The seaward extension of onshore formations and structures were previously almost unknown in Provence. The interpretation of 2D high-resolution marine seismic profiles together with the integration of sea-bottom rock samples provides new insights into the stratigraphic, structural and paleogeographic framework of pre-Messinian Salinity Crisis (MSC) deposits of the Provence continental shelf. Seven post-Jurassic seismic units have been identified on seismic profiles, mapped throughout the offshore Provence area and correlated with the onshore series. The studied marine surface and sub-surface database provided new insights into the mid and late Cretaceous paleogeography and structural framework as well as into the syn-and post-rift deformation in Provence. Thick (up to 2000 m) Aptian-Albian series whose deposition is controlled by E-W-trending faults are evidenced offshore. The occurrence and location of the Upper Cretaceous South-Provence basin is confirmed by the thick (up to 1500 m) basinal series downlaping the Aptian-Albian unit. This basin was fed in terrigenous sediments by a southern massif (" Massif Méridional ") whose present-day relict is the Paleozoic basement and its sedimentary cover from the Sicié imbricate. In the bay of Marseille, thick syn-rift (Rupelian to Aquitanian) deposition occurred (>1000 m). During the rifting phase, syn-sedimentary deformations consist of dominant N040 to N060 sub-vertical faults with a normal component and N050 drag-synclines and anticlines. The syn-rift and early post-rift units (Rupelian to early Burdigalian) are deformed and form a set of E-W-trending en echelon folds that may result from sinistral strike-slip reactivation of N040 to N060 normal faults during a N-S com-pressive phase of early-to-mid Burdigalian age (18-20 Ma). Finally, minor fault reactivation and local folding affect post-rift deposits within a N160-trending corridor localized south of La Couronne, and could result from a later, post-Burdigalian and pre-Pliocene compressive phase.
Cadre tectonostratigraphique des formations anté-pliocènes du plateau continental de Provence (golfe du Lion oriental, SE France) Mots-clés. – Géologie marine, Tectonostratigraphie, Stratigraphie sismique, Paléogéographie crétacée, Rifting oligo-miocène, Compression post-rift Résumé. – Le prolongement en mer des formations et des structures provençales était jusqu'alors largement inconnu.L'interprétation de profils de sismique-réflexion marine 2D et l'intégration de dragages et carottages du fond-marin ont permis d'apporter des éléments nouveaux concernant le cadre stratigraphique, structural et paléogéographique des dé-pôts anté-Messiniens du plateau continental de Provence. Sept unités sismiques post-jurassiques ont été identifiées sur les profils sismiques, cartographiées à travers le plateau continental et corrélées avec les séries affleurant à terre. Une épaisse unité apto-albienne (jusqu'à 2000 m) dont le dépôt est contrôlé par des failles E-W a été mise en évidence en mer. L'existence d'un bassin subsident au Crétacé supérieur sur le plateau continental de Provence a été confirmée par le développement d'une épaisse série de bassin (jusqu'à 1500 m) reposant en downlap sur l'Apto-Albien. Le socle mé-tamorphique de l'écaille de Sicié et sa couverture sédimentaire peut être considéré comme une relique allochtone du « Massif Méridional » ayant alimenté le Bassin sud-provençal en éléments terrigènes au Crétacé supérieur. Dans la rade de Marseille, une épaisse unité syn-rift (Rupélien à Aquitanien) a été mise en évidence (>1000 m), structurée en une série de synformes en échelon d'axe E-W et affectée par des failles d'orientation dominante N040 à N060. Sur le flanc sud des synformes, la formation de plis d'axe N050 est associée à une déformation syn-sédimentaire. La formation des synformes en échelon d'axe E-W résulterait d'une réactivation en décrochement des failles N040 à N060 pendant une phase de compression N-S dont l'âge est compris entre le Burdigalien inférieur et moyen (18-20 Ma). Enfin, les dépôts post-rift sont affectés par des déformations mineures (réactivation de failles et plis associés), le long d'un couloir d'orientation N160 à l'ouest de la baie de Marseille, attribuables à une phase de compression post-burdigalienne et anté-pliocène.
ISSN: 0037-9409

hal-01463727
https://hal-amu.archives-ouvertes.fr/hal-01463727
https://hal-amu.archives-ouvertes.fr/hal-01463727/document
https://hal-amu.archives-ouvertes.fr/hal-01463727/file/Fournier_et_al_Provence_2016.pdf
DOI : 10.2113/gssgfbull.187.4-5.187

Éditeur(s) : HAL CCSD American Geophysical Union
Résumé : International audience
Seismic anisotropy has been widely studied this last decade, particularly by measuring splitting of vertically propagating core shear waves. The main interest in this technique is to characterize upper mantle flow beneath seismic stations. On the other hand, the major restriction in this method is that a single station gives a single anisotropy measurement. Alternative methods have been developed in order to avoid this restriction. An accurate determination of upper mantle seismic anisotropy beneath a seismic station may allow one, by doing anisotropy correction, to characterize remote or deeper anisotropy. The Geoscope network is ideal for this purpose because it is composed of a large set (about 26) of high-quality, broadband seismometers globally distributed and because some of these stations have run for more than 10 years and most of them for more than 5 years. We selected about 100 events at each site, generally of magnitude (m b) > 6.0, and we performed systematic measurements of the splitting parameters (fast polarization direction φ and delay time δt) on SKS, SKKS, and PKS phases. Splitting on oceanic islands has been difficult to observe owing to the low quality of the signal but also perhaps owing to complex upper mantle structures beneath the stations. Station KIP (Kipapa, Hawaii) in the Pacific is the only oceanic Geoscope station with a clear anisotropy. We determined well-constrained splitting parameters for 10 of the 17 continental stations that may be explained by a single anisotropic layer. The poor correlation between fast polarization directions and the absolute plate motion together with the apparent incoherence between the plate velocities and the observed delay times suggest that a simple drag-induced asthenospheric flow alone fails to explain most of the observations. For some stations located on or near major lithospheric structures (TAM, Tamanrasset, Algeria, for instance), we observe a good correlation between fast polarization directions and regional structures. At station SCZ (Santa Cruz, California), we found clear variations of the splitting parameters as a function of the event backazimuth, compatible with two layers of anisotropy. Three stations (CAN (Canberra), HYB (Hyderabad, India) and SSB (Saint Sauveur Badole, France)) seem to be devoid of detectable anisotropy.
ISSN: 2169-9313

hal-01388830
http://hal.univ-reunion.fr/hal-01388830
http://hal.univ-reunion.fr/hal-01388830/document
http://hal.univ-reunion.fr/hal-01388830/file/Barruol_anisoGeoscope_JGR1999.pdf
DOI : 10.1029/1999JB900033

Éditeur(s) : Actes de colloques. Ifremer. Brest [ACTES COLLOQ. IFREMER.]. 1995
Résumé : Informations gathered in the mangrove of the Fort-De-France bay of Martinique (F.W.I.) showed a decreased physiological condition of Crassostrea rhizophorae in the south part of the bay explained by the presence of zinc and by physico-chemical parameters modified in the wet season, such as decreased salinity and decreased levels of nutrients. Bioaccumulation of zinc, lead and cadmium was highly correlated to sediment contamination. Contamination by pesticides and organochlorides was found mainly in oysters from the North part of the bay, characterized by industrial activities and agricultural inputs.

Droits : info:eu-repo/semantics/openAccess
http://archimer.ifremer.fr/doc/1992/acte-1601.pdf
http://archimer.ifremer.fr/doc/00000/1601/

Éditeur(s) : HAL CCSD American Chemical Society
Résumé : International audience
Chlordecone is an organochlorine pesticide that was extensively used in the French West Indies to fight weevils in banana plantations from 1973 to 1993. This has led to a persistent pollution of the environment and to the contamination of the local population for several decades with effects demonstrated on human health. Chlordecone accumulates mainly in the liver where it is known to potentiate the action of hepatotoxic agents. However, there is currently no information on its in situ localization in the liver. We have thus evaluated a matrix-assisted laser desorption ionization (MALDI) imaging quantification method based on labeled normalization for the in situ localization and quantification of chlordecone. After validating the linearity and the reproducibility of this method, quantitative MALDI imaging was used to study the accumulation of chlordecone in the mouse liver. Our results revealed that normalized intensities measured by MALDI imaging could be first converted in quantitative units. These quantities appeared to be different from absolute quantities of chlordecone determined by gas chromatography (GC), but they were perfectly correlated (R(2) = 0.995). The equation of the corresponding correlation curve was thus efficiently used to convert quantities measured by MALDI imaging into absolute quantities. Our method combining labeled normalization and calibration with an orthogonal technique allowed the in situ absolute quantification of chlordecone by MALDI imaging. Finally, our results obtained on the pathological mouse liver illustrate the advantages of quantitative MALDI imaging which preserves information on in situ localization without radioactive labeling and with a simple sample preparation.
ISSN: 0003-2700

hal-01118103
https://hal-univ-rennes1.archives-ouvertes.fr/hal-01118103
PUBMED : 24837422
DOI : 10.1021/ac500313s

Éditeur(s) : HAL CCSD Nature Publishing Group
Résumé : International audience
Lateral variations along the Himalayan arc are suggested by an increasing number of studies and carry important information about the orogen’s segmentation. Here we compile the hitherto most complete land gravity dataset in the region which enables the currently highest resolution plausible analysis. To study lateral variations in collisional structure we compute arc-parallel gravity anomalies (APaGA) by subtracting the average arc-perpendicular profile from our dataset; we compute likewise for topography (APaTA). We find no direct correlation between APaGA, APaTA and background seismicity, as suggested in oceanic subduction context. In the Himalayas APaTA mainly reflect relief and erosional effects, whereas APaGA reflect the deep structure of the orogen with clear lateral boundaries. Four segments are outlined and have disparate flexural geometry: NE India, Bhutan, Nepal & India until Dehradun, and NW India. The segment boundaries in the India plate are related to inherited structures, and the boundaries of the Shillong block are highlighted by seismic activity. We find that large earthquakes of the past millennium do not propagate across the segment boundaries defined by APaGA, therefore these seem to set limits for potential rupture of megathrust earthquakes.
ISSN: 2045-2322

hal-01420404
https://hal.archives-ouvertes.fr/hal-01420404
https://hal.archives-ouvertes.fr/hal-01420404/document
https://hal.archives-ouvertes.fr/hal-01420404/file/hetenyi2016.pdf
DOI : 10.1038/srep33866

Éditeur(s) : HAL CCSD American Chemical Society
Résumé : International audience
The HIWATE (Health Impacts of long-term exposure to disinfection byproducts in drinking WATEr) project was a systematic analysis that combined the epidemiology on adverse pregnancy outcomes and other health effects with long-term exposure to low levels of drinking water disinfection byproducts (DBPs) in the European Union. The present study focused on the relationship of the occurrence and concentration of DBPs with in vitro mammalian cell toxicity. Eleven drinking water samples were collected from five European countries. Each sampling location corresponded with an epidemiological study for the HIWATE program. Over 90 DBPs were identified; the range in the number of DBPs and their levels reflected the diverse collection sites, different disinfection processes, and the different characteristics of the source waters. For each sampling site, chronic mammalian cell cytotoxicity correlated highly with the numbers of DBPs identified and the levels of DBP chemical classes. Although there was a clear difference in the genotoxic responses among the drinking waters, these data did not correlate as well with the chemical analyses. Thus, the agents responsible for the genomic DNA damage observed in the HIWATE samples may be due to unresolved associations of combinations of identified DBPs, unknown emerging DBPs that were not identified, or other toxic water contaminants. This study represents the first to integrate quantitative in vitro toxicological data with analytical chemistry and human epidemiologic outcomes for drinking water DBPs.
ISSN: 0013-936X

hal-00875923
https://hal.archives-ouvertes.fr/hal-00875923
DOI : 10.1021/es3024226
PUBMED : 22958121

Éditeur(s) : HAL CCSD BioMed Central
Résumé : Le régime de reproduction de Dicorynia guianensis (caesalpiniaceae), arbre de la forêt guyanaise a été étudié à l'aide des marqueurs enzymatiques. Le matériel végétal a été collecté en 1993 et 1995, sur le dispositif sylvicole de Paracou. Le taux d'allofécondation moyen de la population est élevé, tm = 0,85, stable d'une année à l'autre malgré une variation importante du taux de floraison. Le taux individuel varie entre les arbres mais aucune corrélation n'a pu être établie avec des facteurs spatiaux, densité de floraison ou distance aux plus proches voisins. L'hétérogénéité des nuages polliniques est sensible au niveau des agrégats et des arbres individuels, échelle spatiale où se déploie l'activité des insectes pollinisateurs. Nous n'avons en évidence aucune corrélation spatiale entre compositions des nuages polliniques individuels. Des distances de migration du pollen de 190 mètres ont été mise en évidence grâce aux allèles rares. Aucune différence significative du régime de reproduction de D. guianensis n'a été trouvée entre parcelles traitées et parcelles témoin du dispositif.
ISSN: 0999-193X

hal-01032370
https://hal.archives-ouvertes.fr/hal-01032370

Éditeur(s) : HAL CCSD
Résumé : International audience
The authors analyze the data concerning 219 sera, that they have tested in passive hemagglutination, with as antigen a Dirofilaria immitis proteic extract, in French Polynesia. Besides its light sensitivity, this method does appear very interesting. It points out: --First, that there is a gaussian distribution of the results found at various dilutions, both by "filarian" and "non filarian" people. Therefore, this distribution is a feasible and practical parameter for evaluation of the Filariasis transmission in different populations. --On the other hand, that there is an inverse correlation between the presence of microfilaria in the blood and the level of immunological response for lymphatic Filariasis.
Bull Soc Pathol Exot Filiales

hal-00715412
https://hal.univ-antilles.fr/hal-00715412
PUBMED : 1037386

Éditeur(s) : HAL CCSD Springer Verlag
Résumé : International audience
Continuous Global Positioning System (CGPS) position time series from eastern North America constrain the pattern and magnitude of regional crustal deformation. Initial analysis delineates consistent uplift patterns, as expected from glacial isostatic adjustment (GIA) predictions, but the associated horizontal deformation is not definitive, in part due to the short time periods for a significant number of the available stations. We employ an eigenpattern decomposition in order to define a unique, finite set of deformation patterns for this continuous GPS data. Similar in nature to the empirical orthogonal functions historically employed in the analysis of atmospheric and oceanographic phenomena, the method derives the eigenvalues and eigenstates from the diagonalization of the correlation matrix using a Karhunen-Loeve expansion (KLE). The KLE technique is used to identify the important modes in both time and space for the CGPS data, modes that potentially include signals such as horizontal and vertical GIA, tectonic strain, and seasonal effects. Here we filter both the vertical and horizontal velocity patterns on different spatiotemporal scales in order to study the potential geophysical sources, after the removal of correlated and random noise. The method is successful in disaggregating the linear vertical signal from more variable and less spatially correlated signals. The vertical and horizontal results are compared to the predictions of the ICE-3G GIA loading model with a number of plausible mantle viscosity profiles. The horizontal velocity analysis allows for qualitative differentiation between several potential GIA models and suggests that, with longer time series, this technique can be employed to remove correlated noise and improve estimates of crustal strain patterns and their sources.
ISSN: 0033-4553

hal-00795454
https://hal.archives-ouvertes.fr/hal-00795454
DOI : 10.1007/s00024-011-0420-1

Éditeur(s) : HAL CCSD
Résumé : International audience
MOTIVATION: Imaging mass spectrometry (IMS) is a maturating technique of molecular imaging. Confidence in the reproducible quality of IMS data is essential for its integration into routine use. However, the predominant method for assessing quality is visual examination, a time consuming, unstandardized and non-scalable approach. So far, the problem of assessing the quality has only been marginally addressed and existing measures do not account for the spatial information of IMS data. Importantly, no approach exists for unbiased evaluation of potential quality measures. RESULTS: We propose a novel approach for evaluating potential measures by creating a gold-standard set using collective expert judgements upon which we evaluated image-based measures. To produce a gold standard, we engaged 80 IMS experts, each to rate the relative quality between 52 pairs of ion images from MALDI-TOF IMS datasets of rat brain coronal sections. Experts' optional feedback on their expertise, the task and the survey showed that (i) they had diverse backgrounds and sufficient expertise, (ii) the task was properly understood, and (iii) the survey was comprehensible. A moderate inter-rater agreement was achieved with Krippendorff's alpha of 0.5. A gold-standard set of 634 pairs of images with accompanying ratings was constructed and showed a high agreement of 0.85. Eight families of potential measures with a range of parameters and statistical descriptors, giving 143 in total, were evaluated. Both signal-to-noise and spatial chaos-based measures performed highly with a correlation of 0.7 to 0.9 with the gold standard ratings. Moreover, we showed that a composite measure with the linear coefficients (trained on the gold standard with regularized least squares optimization and lasso) showed a strong linear correlation of 0.94 and an accuracy of 0.98 in predicting which image in a pair was of higher quality. AVAILABILITY AND IMPLEMENTATION: The anonymized data collected from the survey and the Matlab source code for data processing can be found at: https://github.com/alexandrovteam/IMS_quality. CONTACT: theodore.alexandrov@embl.de
Bioinformatics (Oxford, England)

hal-01163754
https://hal-univ-rennes1.archives-ouvertes.fr/hal-01163754
DOI : 10.1093/bioinformatics/btv266
PUBMED : 26072506

Éditeur(s) : HAL CCSD Nature Publishing Group
Résumé : International audience
Marine biological invasions threaten biodiversity worldwide. Here we explore how Marine Protected areas, by reducing human use of the coast, confer resilience against the introduction of non-indigenous species (NIS), using two very different Pacific islands as case studies for developing and testing mathematical models. We quantified NIS vectors and promoters on Vancouver (Canada) and Moorea (French Polynesia) islands, sampled and barcoded NIS, and tested models at different spatial scales with different types of interaction among vectors and between marine protection and NIS frequency. In our results NIS were negatively correlated with the dimension of the protected areas and the intensity of the protection. Small to medium geographical scale protection seemed to be efficient against NIS introductions. The likely benefit of MPAs was by exclusion of aquaculture, principally in Canada. These results emphasize the importance of marine protected areas for biodiversity conservation, and suggest that small or medium protected zones would confer efficient protection against NIS introduction.
ISSN: 2045-2322

hal-01381251
https://hal-univ-perp.archives-ouvertes.fr/hal-01381251
https://hal-univ-perp.archives-ouvertes.fr/hal-01381251/document
https://hal-univ-perp.archives-ouvertes.fr/hal-01381251/file/rate%20of%20biological%20invasions.pdf
DOI : 10.1038/srep33013

Éditeur(s) : HAL CCSD BioMed Central
Résumé : International audience
UNLABELLED: ABSTRACT: BACKGROUND: Malaria remains a major health problem in French Guiana, with a mean of 3800 cases each year. A previous study in Camopi, an Amerindian village on the Oyapock River, highlighted the major contribution of environmental features to the incidence of malaria attacks. We propose a method for the objective selection of the best multivariate peridomestic landscape characterisation that maximises the chances of identifying relationships between environmental features and malaria incidence, statistically significant and meaningful from an epidemiological point of view. METHODS: A land-cover map, the hydrological network and the geolocalised inhabited houses were used to characterise the peridomestic landscape in eleven discoid buffers with radii of 50, 100, 200, 300, 400, 500, 600, 700, 800, 900 and 1000 metres. Buffer-based landscape characterisations were first compared in terms of their capacity to discriminate between sites within the geographic space and of their effective multidimensionality in variable space. The Akaike information criterion (AIC) was then used to select the landscape model best explaining the incidences of P. vivax and P. falciparum malaria. Finally, we calculated Pearson correlation coefficients for the relationships between environmental variables and malaria incidence, by species, for the more relevant buffers. RESULTS: The optimal buffers for environmental characterisation had radii of 100 m around houses for P. vivax and 400 m around houses for P. falciparum. The incidence of P. falciparum malaria seemed to be more strongly linked to environmental features than that of P. vivax malaria, within these buffers. The incidence of P. falciparum malaria in children was strongly correlated with proportions of bare soil (r = -0.69), land under high vegetation (r = 0.68) and primary forest (r = 0.54), landscape division (r = 0.48) and the number of inhabited houses (r = -0.60). The incidence of P. vivax malaria was associated only with landscape division (r = 0.49). CONCLUSIONS: The proposed methodology provides a simple and general framework for objective characterisation of the landscape to account for field observations. The use of this method enabled us to identify different optimal observation horizons around houses, depending on the Plasmodium species considered, and to demonstrate significant correlations between environmental features and the incidence of malaria.
ISSN: 1476-072X

inserm-00674075
http://www.hal.inserm.fr/inserm-00674075
http://www.hal.inserm.fr/inserm-00674075/document
http://www.hal.inserm.fr/inserm-00674075/file/1476-072X-10-65.pdf
DOI : 10.1186/1476-072X-10-65

Éditeur(s) : HAL CCSD IOS Press
Résumé : International audience
The hematocrit-to-viscosity ratio (HVR) has been widely used has an estimate of red blood cell (RBC) oxygen transport effectiveness into the microvasculature or as an oxygen delivery index. However, no study investigated the possibility of HVR to truly reflect RBC oxygen transport effectiveness or to be an oxygen delivery index. We measured blood viscosity at high shear rate (225 s −1), hematocrit, HVR, as well as the microvascular oxyhemoglobin saturation (TOI; tissue oxygen index) by spatial resolved near-infrared spectroscopy (NIRS) at cerebral and muscle levels in three population known to have various degrees of hemorheological abnormalities: healthy subjects (AA), patients with sickle cell SC disease (SC) characterized by moderate anemia and patients with sickle cell anemia (SS) marked by severe anemia. At both the cerebral and muscle level, HVR was positively correlated with TOI (r = 0.28; p = 0.03 and r = 0.38; p = 0.003, at the cerebral and muscle level, respectively). These findings suggest that HVR probably play a key role in blood flow and hemodynamic regulation in the microvasculature, hence modulating the amount of oxygen available for tissues. Nevertheless, the strengths of the associations are weak (R 2 < 0.50), suggesting that other determinants modulate microvascular blood flow and oxygenation, such as vascular geometry and vasomotor reserve.
ISSN: 1386-0291

hal-01163460
https://hal.univ-antilles.fr/hal-01163460
DOI : 10.3233/CH-131742

Éditeur(s) : EDP Sciences
Résumé : Batch fecundity and relative fecundity of swordfish (Xiphias gladius) in the southwestern Indian Ocean were estimated from seven gravid swordfish females (size range 127-225 cm lower jaw-to-fork length, LJFL) with unovulated, hydrated oocytes collected onboard Reunion-based (France) longline swordfish fishing vessels between December 1999 to January 2001. To investigate the spawning pattern of swordfish, we used data collected through a combination of two at sea sampling regimes. A total of 17 007 geo-located size data of swordfish were recorded during 8 years (1993-2001) and a total of 1727 (size range 75-289 cm, LJFL) swordfish gonads (1107 females and 620 males) were sampled from May 1998 to January 2001. The estimated batch fecundity ranged from 995 000 hydrated oocytes for the smallest ripe female to 4.3 millions for the largest female sampled measuring respectively 127 to 225 cm in curved length (LJFL). The relative fecundity ranged from 25 to 72 hydrated oocytes per gram of body weight. We found that batch fecundity was positively correlated with fish length and that the older/larger females have earlier and longer spawning seasons than younger/smaller females. These findings suggested that older/larger females which are seasonally migrating in this spawning ground seem to play a major role in reproductive success of the species in producing significantly more offspring than younger females during an extended spawning season. Examination of the length-frequency date from the fishery indicated that the young fish are resident around Reunion and around the seamounts off Reunion Island. Our results highlight the important role of the older/larger females in the reproductive capacity of southwestern Indian Ocean stock. We discuss the potential implications of fishing the older/larger females for this stock in terms of reproduction and recruitment.
La fécondité par acte de ponte et la fécondité relative chez l'espadon (Xiphias gladius) dans le sud-ouest de l'océan Indien sont estimées d'après sept femelles de taille comprise entre 127 et 225 cm (de l'extrémité de la mâchoire inférieure à la fourche caudale) ; celles-ci ayant atteint la maturité sexuelle, les ovaires contenant des ovocytes hydratés. Ces femelles ont été pêchées entre décembre 1999 et janvier 2001, par des palangriers ciblant l'espadon et basés à La Réunion. La stratégie de la reproduction chez l'espadon a été étudiée en utilisant des données collectées au cours de deux campagnes d'échantillonnages en mer : soit 17 007 mensurations géo-référencées d'espadons, de 75 à 289 cm, qui ont été enregistrées durant les 8 années d'étude (1993-2001) et 1727 gonades d'espadon (1107 femelles et 620 mâles) prélevées entre mai 1998 et juin 2001.La fécondité par acte de ponte estimée varie de 995 000 ovocytes hydratés, pour la plus petite femelle (127 cm), à 4,3 millions pour la plus grande femelle (225 cm). La fécondité par acte de ponte est corrélée positivement à la taille du poisson. La fécondité relative s'étend de 25 à 72 ovocytes hydratés par gramme de poids de corps. Les femelles les plus âgées/grandes pondent plus tôt et sur une période plus grande que les plus jeunes/petites femelles. Ainsi, les femelles plus âgées/grandes qui migrent de façon saisonnière vers cette aire de ponte, contribueraient plus largement au renouvellement de l'espèce en produisant de façon significative davantage de recrues que les femelles plus jeunes et ceci pendant une saison de ponte plus étendue. Par ailleurs, les jeunes individus semblent séjourner aux abords de l'île de La Réunion et des monts sous-marins situés au large. Nos résultats soulignent l'importance du rôle des femelles âgées/grandes dans la capacité reproductrice du stock d'espadon du sud-ouest de l'océan Indien. Les implications potentielles de la pêche : des femelles âgées et de grandes tailles sont discutées pour ce stock, du point de vue de la reproduction et du recrutement.
Aquatic Living Resources (0990-7440) (EDP Sciences), 2009 , Vol. 22 , N. 1 , P. 59-68

Droits : 2009 EDP Sciences
http://archimer.ifremer.fr/doc/2009/publication-6354.pdf
DOI:10.1051/alr/2009012
http://archimer.ifremer.fr/doc/00000/6354/

Éditeur(s) : Elsevier
Résumé : Because Vibrio aestuarianus is known to cause serious infections in Pacific oyster Crassostrea gigas, a real-time PCR assay was developed targeting the dnaJ gene of this bacterium. Only V. aestuarianus strains isolated from C. gigas mortality events in different geographic areas and the reference strain tested positive, whereas no amplification products was obtained with type strains belonging to 23 other species of Vibrio. Sensitivity and reproducibility of the method were assessed using either seawater or oyster homogenate samples spiked with one V aestuarianus strain. All these samples were stored at -20 degrees C in order to mimic retrospective or grouped natural sample analysis without quantification bias due to prolonged freezing. Analysis of standard curves revealed excellent correlation values between light microscopy cell enumerations and PCR Threshold Cycle (Ct) values, and acceptable PCR reaction efficiencies for all type of samples. Quantification curves of both sample types were equivalent, with a detection level as low as 1.6 V. aestuarianus cells in the PCR reaction tube, corresponding to 1.6.10(2) cells ml(-1) and 1.6.10(2) cells mg(-1) in seawater and entire oyster samples, respectively, taking into account the dilution factor used for appropriate template DNA preparation. Comparison of PCR assay reproducibility according to the complexity of samples revealed that seawater samples gave more reproducible quantification measures than samples from oyster homogenate, with precision of measured Ct values inferior to 0.4 and 0.6 respectively at 99% confidence. Use of the real-time PCR assay allowed us to monitor V. aestuarianus load in oysters naturally infected with this pathogen. Furthermore, we were able to detect V aestuarianus in samples of seawater in which oysters had been reared and in algal cultures used for feeding oysters. Because of the rapidity and reliability of the real-time PCR assay method used in this study, just a few hours are needed compared with the two days required using the classic culture method, this technique will be particularly valuable in mollusc pathology laboratories, for monitoring the source and course of infections by V. aestuarianus in pathogenesis and epidemiologic studies, as well as for designing appropriate prophylactic control measures. (C) 2009 Elsevier B.V. All rights reserved.
Journal of Microbiological Methods (0167-7012) (Elsevier), 2009-05 , Vol. 77 , N. 2 , P. 191-197

Droits : 2009 Elsevier B.V. All rights reserved.
http://archimer.ifremer.fr/doc/2009/publication-6447.pdf
DOI:10.1016/j.mimet.2009.01.021
http://archimer.ifremer.fr/doc/00000/6447/

Éditeur(s) : HAL CCSD American Geophysical Union
Résumé : International audience
Detection and attribution of human influence on sea level rise are important topics that have not yet been explored in depth. We question whether the sea level changes (SLC) over the past century were natural in origin. SLC exhibit power law long-term correlations. By estimating Hurst exponent through Detrended Fluctuation Analysis and by applying statistics of Lennartz and Bunde [2009], we search the lower bounds of statistically significant external sea level trends in longest tidal records worldwide. We provide statistical evidences that the observed SLC, at global and regional scales, is beyond its natural internal variability. The minimum anthropogenic sea level trend (MASLT) contributes to the observed sea level rise more than 50% in New York, Baltimore, San Diego, Marseille, and Mumbai. A MASLT is about 1 mm/yr in global sea level reconstructions that is more than half of the total observed sea level trend during the XXth century.
ISSN: 0094-8276

hal-01336190
https://hal.archives-ouvertes.fr/hal-01336190
https://hal.archives-ouvertes.fr/hal-01336190/document
https://hal.archives-ouvertes.fr/hal-01336190/file/BECKER2014a.pdf
DOI : 10.1002/2014GL061027

Éditeur(s) : HAL CCSD Botanical Society of America
Résumé : Premise of the study : Wood density correlates with mechanical and physiological strategies of trees and is important for estimating global carbon stocks. Nonetheless, the relationship between branch and trunk xylem density has been poorly explored in neotropical trees. Here, we examine this relationship in trees from French Guiana and its variation among different families and sites, to improve the understanding of wood density in neotropical forests. Methods : Trunk and branch xylem densities were measured for 1909 trees in seven sites across French Guiana. A major-axis fit was performed to explore their general allometric relationship and its variation among different families and sites. Key results : Trunk xylem and branch xylem densities were significantly positively correlated, and their relationship explained 47% of the total variance. Trunk xylem was on average 9% denser than branch xylem. Family-level differences and interactions between family and site accounted for more than 40% of the total variance, whereas differences among sites explained little variation. Conclusions : Variation in xylem density within individual trees can be substantial, and the relationship between branch xylem and trunk xylem densities varies considerably among families and sites. As such, whole-tree biomass estimates based on non-destructive branch sampling should correct for both taxonomic and environmental factors. Furthermore, detailed estimates of the vertical distribution of wood density within individual trees are needed to determine the extent to which relying solely upon measures of trunk wood density may cause carbon stocks in tropical forests to be overestimated.
ISSN: 0002-9122

hal-01032401
https://hal.archives-ouvertes.fr/hal-01032401
DOI : 10.3732/ajb.1000034