Éditeur(s) : HAL CCSD Elsevier Masson
Résumé : International audience
Antisynthetase syndrome is an inflammatory myopathy frequently associated with pulmonary manifestations, especially interstitial lung diseases, and uncommonly pulmonary hypertension. In the context of a suggestive clinical and radiological picture, positive anti-RNA synthetase antibodies confirm the diagnosis. Anti-Jo1, anti-PL7, and anti-PL12 antibodies are the more commonly encountered. The presence of a number of extra-thoracic manifestations in association with pulmonary disease may suggest the diagnosis. These include: myalgia or muscular deficit, Raynaud's phenomenon, polyarthritis, fever, mechanics hands. Serum creatine kinase levels are usually increased. Electromyogram, muscular magnetic resonance imaging or muscle pathology are not mandatory to make the diagnosis. There is a high variability in symptoms and severity, between patients but also during the course of the disease in the same patient. The presence of an interstitial lung disease is a major prognostic factor and an indication for more intensive treatment, principally with systemic corticosteroids with or without immunosuppressive drugs. Improving respiratory physicians’ knowledge of this disease, which is often revealed by its pulmonary manifestations, should help diagnosis, therapeutic management, and possibly prognosis.
Le syndrome des antisynthétases correspond à une myopathie inflammatoire fréquemment associée à une atteinte pulmonaire, surtout parenchymateuse (pneumopathie infiltrante diffuse) et plus rarement vasculaire (hypertension pulmonaire). Devant un tableau clinique et radiologique évocateur, la présence d’anticorps anti-ARNt synthétases signe le diagnostic. Les plus fréquents de ces auto-anticorps sont les anti-Jo1, les anti-PL7 et les anti-PL12. De nombreuses manifestations cliniques extrathoraciques associées à l’atteinte pulmonaire peuvent orienter le diagnostic : myalgies ou déficit musculaire, phénomène de Raynaud, polyarthrite, fièvre et « mains de mécaniciens ». Une augmentation des créatine-phospho-kinases est quasi constante. Le recours à l’électromyogramme, à l’imagerie musculaire par résonance magnétique ou à l’histologie n’est pas systématique. Néanmoins, il existe une grande variabilité des symptômes et de leur sévérité d’un patient à l’autre ou au long de l’évolution de la maladie. L’atteinte pulmonaire est un facteur pronostic majeur et incite à traiter de manière intensive les patients, principalement par corticothérapie systémique, voire d’emblée par association corticoïdes et immunosuppresseurs. Mieux connaître cette pathologie qui est souvent de révélation pneumologique permettrait d’améliorer le diagnostic et la prise en charge thérapeutique, voire même le pronostic.
ISSN: 0761-8425

hal-01085369
https://hal-univ-rennes1.archives-ouvertes.fr/hal-01085369
https://hal-univ-rennes1.archives-ouvertes.fr/hal-01085369/document
https://hal-univ-rennes1.archives-ouvertes.fr/hal-01085369/file/Manifestations%20pulmonaires%20du%20syndrome%20des%20antisynth%C3%A9tases_accepted.pdf
DOI : 10.1016/j.rmr.2014.07.013

Éditeur(s) : HAL CCSD Éditions de la Maison des sciences de l'homme
Résumé : International audience
For over a century, the complementary nature of fishing and farming activities in the floodplain of the Rufiji River (Tanzania) has formed the basis of the economy in its villages. Starting in the 1970s, the fishery evolved from a seasonal activity practiced on foot during flooding and using traditional traps to year-round dugout canoe operated lake fisheries using nets.The paper analyses the drivers of this change. After having rejected the simplistic technocratic hypothesis that technical innovation equals progress, the paper examines the relevance of the productivity of the different fishing techniques in the light of the evolution of the role of fisheries in the village economy. During the change-over period, the national and international economy deprives the villages of their main habitual sources of cash income. In the absence of any alternative, fisheries imposed themselves as the unique source of cash income, a function they can only fulfil if practiced year-round and this can only be achieved using canoes and nets. However, the perception of the causes of these changes in the villages is differentiated. For those who initiated the change-over, and who are now of an advanced age, the main driver of the transition to nets was the empowerment of the youth versus the elders. Such an interpretation resonates well with the economic determinants. However, it requires considering the modernisation process as a contextualised multi-sided expression of market globalisation.
Depuis plus d’un siècle, l’économie des villages de la plaine inondable du Rufiji (Tanzanie) est fondée sur la complémentarité des activités agricoles et des activités halieutiques. À partir de la fin des années 1970, les pêches traditionnelles à la nasse, pratiquées à pied saisonnièrement lors de la crue annuelle, sont supplantées par les pêches embarquées (pirogues monoxyles) au filet dans les lacs, pratiquées elles toute l’année. L’article s’interroge sur les déterminants de cette substitution.Après avoir rejeté l’hypothèse techniciste vulgaire qui voudrait que l’innovation technique soit synonyme de progrès, la pertinence du critère de productivité des différentes techniques de pêche est examinée à la lumière de l’évolution de la place des activités halieutiques dans l’économie villageoise. Il apparaît qu’à partir de cette époque, l’évolution économique nationale et internationale prive les villageois de leurs principales ressources monétaires habituelles. En l’absence de toute alternative, la pêche s’impose alors comme unique source de revenu monétaire, une fonction qu’elle ne peut remplir qu’à condition d’être pratiquée tout au long de l’année, ce que seules les techniques au filet permettent. Cependant, les représentations villageoises des causes de la généralisation de la pêche au filet sont différentes. Pour ceux d’entre eux, aujourd’hui très âgés, qui furent les pionniers de cette innovation, c’est le souci d’émancipation des cadets vis-à-vis des aînés qui aurait été le moteur de l’adoption de techniques de pêche au filet. Ce point de vue entre en résonance avec les déterminations économiques à condition de considérer le processus de modernisation comme manifestation multiforme contextualisée de la mondialisation marchande.
ISSN: 0248-6016

hal-01344410
https://hal.univ-antilles.fr/hal-01344410
https://hal.univ-antilles.fr/hal-01344410/document
https://hal.univ-antilles.fr/hal-01344410/file/Pauletal2015Version_Def.pdf

Éditeur(s) : Elsevier Science Bv
Résumé : The red drum Sciaenops ocellatus is an estuarine-dependent sciaenid that has supported important recreational and commercial fisheries for many years. Since the 1980s, this species has been farmed in different regions of the world and studies have been conducted to find natural ways to improve its culture. Sciaenops ocellatus is well known for making calls but studies on this characteristic have been mainly restricted to passive acoustics. The aim of this study was to provide acoustic-related information that could be useful in rearing practices. We have studied in detail fish calling characteristics, described their sound-producing mechanism and tested the recordings in different kinds of confinements (floating cages, concrete and fibreglass tanks). Contrary to previous studies, calls were recorded mainly in the morning, between 06:00 and 09:00. Sounds are made only by males; females do not have sound-producing apparatus. The anatomy, muscle ultrastructure data and calling characteristics show conclusively that calls result from the contraction of high speed muscles which are characterized by the very small diameter (7 μm) of the muscle cells. Preliminary results suggest sound characteristics can give information on the fish physiology but further studies are needed. The effects of resonance of fibreglass tanks are experimentally highlighted, showing unequivocally that all the characteristics of fish calls are completely distorted, even in big tanks (13 m3). These findings should be taken into account when using pre-recorded sounds in the rearing of this species because it can affect communication during courtship activity.
Aquaculture (0044-8486) (Elsevier Science Bv), 2014-08 , Vol. 432 , P. 204-211

Droits : 2014 Elsevier B.V. All rights reserved
http://archimer.ifremer.fr/doc/00190/30127/28589.pdf
DOI:10.1016/j.aquaculture.2014.05.017
http://archimer.ifremer.fr/doc/00190/30127/

Éditeur(s) : HAL CCSD Elsevier
Résumé : International audience
The estrogen receptor (ER) is a transcription factor that binds under 17-b-estradiol (E2) stimulation as homodimer to a short DNA consensus sequence named estrogen response element (ERE). The ER/ERE interaction has been assessed by several research groups through different methodologies notably by surface plasmon resonance (SPR) techniques. The biochemical parameters and conditions (solvent, ER concentration, salt, time and temperature) used to prepare samples before analysis were very different from one study to another. But no studies have aimed to compare the effect of these modifications on ER/ERE interaction. Therefore the main objective of the present paper was to assess the influence of biochemical parameters onto the ER/ERE interaction with the final aim to improve the comprehension of this interaction. Our results highlighted that parameters like solvent, ER concentration, salt and surfactant concentration, temperature and time deeply modify ER/ERE interaction. Nevertheless, the dimer formation under E2 stimulation occurred with all tested conditions. Altogether, incubation parameters of ER with E2, deeply modify its binding level onto ERE. These data constitute an important key point to consider for the improvement of ER/ERE detection method depending upon the aim of the study (interaction measurement, environmental detection, development of new technologies or devices).
ISSN: 0003-9861

hal-00936061
https://hal.archives-ouvertes.fr/hal-00936061
https://hal.archives-ouvertes.fr/hal-00936061/document
https://hal.archives-ouvertes.fr/hal-00936061/file/Impactsur biochemical_design_on_estrogen_receptor_estrogen_response_element_interaction_by_surface_plasmon_resonance_technology-accepted.pdf
DOI : 10.1016/j.abb.2013.11.006

Éditeur(s) : HAL CCSD Elsevier
Résumé : International audience
The structural parameters of fluorinated nanocarbons presenting different shapes, i.e. spherical, tubular and discotic are investigated and correlated to their tribological properties. Different fluorination rates of graphitized carbon blacks (0D), carbon nanofibres (1D) and a mixture of carbon nanodiscs and nanocones (2D) were achieved under pure molecular fluorine gas flow (direct fluorination). Raman spectrometry, X-ray diffraction and 19F solid state nuclear magnetic resonance underline similar structure and nature of the C-F bonds (covalent) for equivalent fluorine contents. In spite of the similarities of physical-chemical properties at equivalent fluorine contents, the tribological properties of the fluorinated nanocarbons differ. Those properties are discussed taking into account the role of the fluorine content and location of the fluorine atoms in the decrease of the interparticle interactions and in the cleavage of the external fluorocarbon layers to form the tribofilm. Finally, the effect of the shape is discussed.
ISSN: 0022-1139

hal-00787267
https://hal.archives-ouvertes.fr/hal-00787267

Éditeur(s) : HAL CCSD Public Library of Science
Résumé : International audience
BACKGROUND: The use of pesticides and the related environmental contaminations can lead to human exposure to various molecules. In early-life, such exposures could be responsible for adverse developmental effects. However, human health risks associated with exposure to complex mixtures are currently under-explored. OBJECTIVE: THIS PROJECT AIMS AT ANSWERING THE FOLLOWING QUESTIONS: What is the influence of exposures to multiple pesticides on the metabolome? What mechanistic pathways could be involved in the metabolic changes observed? METHODS: Based on the PELAGIE cohort (Brittany, France), 83 pregnant women who provided a urine sample in early pregnancy, were classified in 3 groups according to the surface of land dedicated to agricultural cereal activities in their town of residence. Nuclear magnetic resonance-based metabolomics analyses were performed on urine samples. Partial Least Squares Regression-Discriminant Analysis (PLS-DA) and polytomous regressions were used to separate the urinary metabolic profiles from the 3 exposure groups after adjusting for potential confounders. RESULTS: The 3 groups of exposure were correctly separated with a PLS-DA model after implementing an orthogonal signal correction with pareto standardizations (R2 = 90.7% and Q2 = 0.53). After adjusting for maternal age, parity, body mass index and smoking habits, the most statistically significant changes were observed for glycine, threonine, lactate and glycerophosphocholine (upward trend), and for citrate (downward trend). CONCLUSION: This work suggests that an exposure to complex pesticide mixtures induces modifications of metabolic fingerprints. It can be hypothesized from identified discriminating metabolites that the pesticide mixtures could increase oxidative stress and disturb energy metabolism.
ISSN: 1932-6203

inserm-00863407
http://www.hal.inserm.fr/inserm-00863407
http://www.hal.inserm.fr/inserm-00863407/document
http://www.hal.inserm.fr/inserm-00863407/file/journal.pone.0064433.pdf
PUBMED : 23704985

Éditeur(s) : HAL CCSD Elsevier
Résumé : International audience
Magnetic Resonance Imaging (MRI), a reference examination for cardiac morphology and function in humans, allows to image the cardiac right ventricle (RV) with high spatial resolution. The segmentation of the RV is a difficult task due to the variable shape of the RV and its ill-defined borders in these images. The aim of this paper is to evaluate several RV segmentation algorithms on common data. More precisely, we report here the results of the Right Ventricle Segmentation Challenge (RVSC), concretized during the MICCAI'12 Conference with an on-site competition. Seven automated and semi-automated methods have been considered, along them three atlas-based methods, two prior based methods, and two prior-free, image-driven methods that make use of cardiac motion. The obtained contours were compared against a manual tracing by an expert cardiac radiologist, taken as a reference, using Dice metric and Hausdorff distance. We herein describe the cardiac data composed of 48 patients, the evaluation protocol and the results. Best results show that an average 80% Dice accuracy and a 1cm Hausdorff distance can be expected from semi-automated algorithms for this challenging task on the datasets, and that an automated algorithm can reach similar performance, at the expense of a high computational burden. Data are now publicly available and the website remains open for new submissions (http://www.litislab.eu/rvsc/).
ISSN: 1361-8415

hal-01141170
https://hal.archives-ouvertes.fr/hal-01141170
PUBMED : 25461337

Éditeur(s) : HAL CCSD
Résumé : International audience
Background/AimEpidemiological studies reported that prenatal solvent exposure was associated with adverse neurobehavioral outcomes that suggest impaired motor inhibition function. Using functional Magnetic Resonance Imaging (fMRI) among children aged 10-12 years old, we aimed at investigating the effect of prenatal exposure to solvents on the activation of cerebral regions involved in the motor inhibition function.
International Society of Environmental Epidemiology
Sydney, Australia

inserm-01578506
http://www.hal.inserm.fr/inserm-01578506

Éditeur(s) : HAL CCSD Taylor & Francis (Routledge)
Résumé : International audience
In this study, we analyzed the correlations between hip flexion power, sprint performance, lumbar lordosis (LL) and the cross-sectionalarea (CSA) of the psoas muscle (PM). Ten young adults performed isokinetic tests to determine hip flexion power and two sprinttests. Magnetic resonance imaging was used to determine LL and PM CSA. There were correlations between hip flexion power, sprintperformance, and PM CSA, but LL showed no correlation with any parameter. The impact of hip flexion power and LL on sprintstride pattern efficiency was considered. Hip flexion might not have a simple role in the passive knee replacement of the stride pattern;instead, it may be an active parameter. Other investigations are needed to determine the influence of pelvic architecture on sprintperformance.
ISSN: 0270-1367

hal-01143531
https://hal.univ-antilles.fr/hal-01143531

Éditeur(s) : Wiley-blackwell
Résumé : Aims Exopolysaccharides (EPS) are industrially valuable molecules with numerous useful properties. This study describes the techniques used for the identification of a novel Vibrio bacterium and preliminary characterization of its EPS. Methods and Results Bioprospection in marine intertidal areas of New Caledonia followed by screening for EPS producing brought to selection of the isolate NC470. Phylogenetic analysis (biochemical tests, gene sequencing and DNADNA relatedness) permitted to identify NC470 as a new member of the Vibrio genus. The EPS was produced in batch fermentation, purified using the ultrafiltration process and analysed by colorimetry, Fourier Transform Infrared spectroscopy, gas chromatography, Nuclear Magnetic Resonance and HPLC-size exclusion chromatography. This EPS exhibits a high N-acetyl-hexosamines and uronic acid content with a low amount of neutral sugar. The molecular mass was 672x103Da. These data are relevant for possible technological exploitation. Conclusions We propose the name Vibrio neocaledonicus sp. nov for this isolate NC470, producing an EPS with an unusual sugar composition. Comparison with other known polymers permitted to select applications for this polymer. Significance and Impact of the Study This study contributes to evaluate the marine biodiversity of New Caledonia. It also highlights the biotechnological potential of New Caledonia marine bacteria.
Journal Of Applied Microbiology (1364-5072) (Wiley-blackwell), 2013-06 , Vol. 114 , N. 6 , P. 1702-1712

Droits : 2013 The Society for Applied Microbiology
http://archimer.ifremer.fr/doc/00144/25501/24036.pdf
DOI:10.1111/jam.12184
http://archimer.ifremer.fr/doc/00144/25501/

Éditeur(s) : ISTPM
Résumé : The "Thalassa" undertook a cruise to measure hydrological parameters between the coasts of Algeria and the 40th parallel. A series of deep-sea samples were taken at successive depths to study zooplankton, which has been little studied in this region (Fig. A)....
La «Thalassa» a procédé en janvier 1961 à une campagne hydrologique entre la côte algérienne et le 40• parallèle. Dans son programme a été incluse une série de pêches profondes, par paliers successifs, qu'il nous a paru intéressant de faire en cette région où le zooplancton n'a été jusqu'ici que très peu étudié (fig. A). Cette opération devait être effectuée avec le filet préconisé par la Commission internationale pour l'Exploration de la Méditerranée, mais la perte de cet engin quelques jours plus tôt obligea à utiliser un filet fermant « Discovery ». De toute manière, les échantillons ainsi prélevés étant comparables entre eux, il nous était possible d'évaluer la richesse relative des différents niveaux prospectés. On ne pouvait s'attendre à trouver dans ce secteur de haute mer une variété et une densité du même ordre que celle des secteurs périphériques, sachant qu'en cette région les courants et autres échanges hydrologiques sont moindres qu'au voisinage des côtes ou dans les golfes comme ceux de Gênes ou du Lion. L'hydrologie faite par J. FURNESTIN et Ch. ALLAIN (1962) est venue renforcer cette opinion. Si les mélanges et les mouvements des eaux de divers types y sont d'un grand intérêt, ils se manifestent surtout en profondeur, au-dessous de 1 000 m et leur résonance en surface est affaiblie; quant à la couche superficielle, elle est, là comme ailleurs, influencée par les dérivations du courant atlantique qui n'y provoquent pas de contrastes aussi nets qu'à la périphérie du Bassin. C'est sans doute, compte tenu de l'imperfection qui s'attache aux pêches profondes avec l'engin de faible rendement qu'est le filet à plancton, la raison pour laquelle l'analyse quantitative du zooplancton aussi bien que celle des groupes particulièrement examinés ici (Copépodes et Chaetognathes) ne nous a apporté que des résulta ts relativement banals, Et ceux-ci seraient en somme assez décevants si, par certaines anomalies de la répartition des espèces, ils ne venaient confirmer la valeur des planctontes en tant qu'indicateurs des phénomènes hydrologiques méditerranéens (courant superficiel et mouvements profonds de convection). (OCR non contrôlé)
Revue des Travaux de l'Institut des Pêches Maritimes (0035-2276) (ISTPM), 1962-09 , Vol. 26 , N. 3 , P. 320-368

Droits : Ifremer
http://archimer.ifremer.fr/doc/1962/publication-4191.pdf
http://archimer.ifremer.fr/doc/00000/4191/

Éditeur(s) : HAL CCSD Oxford University Press (OUP)
Résumé : International audience
Sensitive instruments like strainmeters and tiltmeters are necessary for measuring slowly varying low amplitude Earth deformations. Nonetheless, laser and fibre interferometers are particularly suitable for interrogating such instruments due to their extreme precision and accuracy. In this paper, a practical design of a simple pendulum borehole tiltmeter based on laser fibre interferometric displacement sensors is presented. A prototype instrument has been constructed using welded borosilicate with a pendulum length of 0.85 m resulting in a main resonance frequency of 0.6 Hz. By implementing three coplanar extrinsic fibre Fabry-Perot in-terferometric probes and appropriate signal filtering, our instrument provides tilt measurements that are insensitive to parasitic deformations caused by temperature and pressure variations. This prototype has been installed in an underground facility (Rustrel, France) where results show accurate measurements of Earth strains derived from Earth and ocean tides, local hydro-logic effects, as well as local and remote earthquakes. The large dynamic range and the high sensitivity of this tiltmeter render it an invaluable tool for numerous geophysical applications such as transient fault motion, volcanic strain and reservoir monitoring.
ISSN: 0956-540X

hal-01265967
https://hal.archives-ouvertes.fr/hal-01265967
https://hal.archives-ouvertes.fr/hal-01265967/document
https://hal.archives-ouvertes.fr/hal-01265967/file/HCS1.pdf
DOI : 10.1093/gji/ggv358

Éditeur(s) : HAL CCSD De Gruyter
Résumé : International audience
Wallaba (Eperua falcata) is a tropical wood which is known to have naturally low swelling characteristics. Samples of wallaba heartwood were subjected to differential solvent extraction. Wood pieces that were extracted with methanol experienced significantly higher swelling after rehydration from oven dry to 96% relative humidity in comparison to non-extracted samples and samples extracted with other solvents. Methanol soluble wallaba heartwood extract was purified by high pressure liquid chromatography and the compounds present were characterised by nuclear magnetic resonance spectroscopy. Methanol extract was found to contain a very high relative proportion of polar compounds which are proposed to bind to the polymeric cell wall by multiple hydrogen bonds restricting the association of water and therefore acting as natural anti-swelling agents.
ISSN: 0018-3830

hal-00856942
https://hal.archives-ouvertes.fr/hal-00856942
https://hal.archives-ouvertes.fr/hal-00856942/document
https://hal.archives-ouvertes.fr/hal-00856942/file/Chemical_extractives_tropical_wood_Royer_al.pdf

Éditeur(s) : HAL CCSD Elsevier
Résumé : International audience
: Previously, we demonstrated that eicosapentaenoic acid enhanced ethanol-induced oxidative stress and cell death in primary rat hepatocytes via an increase in membrane fluidity and lipid raft clustering. In this context, another n-3 polyunsaturated fatty acid, docosahexaenoic acid (DHA), was tested with a special emphasis on physical and chemical alteration of lipid rafts. Pretreatment of hepatocytes with DHA reduced significantly ethanol-induced oxidative stress and cell death. DHA protection could be related to an alteration of lipid rafts. Indeed, rafts exhibited a marked increase in membrane fluidity and packing defects leading to the exclusion of a raft protein marker, flotillin. Furthermore, DHA strongly inhibited disulfide bridge formation, even in control cells, thus suggesting a disruption of protein-protein interactions inside lipid rafts. This particular spatial organization of lipid rafts due to DHA subsequently prevented the ethanol-induced lipid raft clustering. Such a prevention was then responsible for the inhibition of phospholipase C-γ translocation into rafts, and consequently of both lysosome accumulation and elevation in cellular low-molecular-weight iron content, a prooxidant factor. In total, the present study suggests that DHA supplementation could represent a new preventive approach for patients with alcoholic liver disease based upon modulation of the membrane structures.
ISSN: 0278-6915

hal-00860153
https://hal-univ-rennes1.archives-ouvertes.fr/hal-00860153
https://hal-univ-rennes1.archives-ouvertes.fr/hal-00860153/document
https://hal-univ-rennes1.archives-ouvertes.fr/hal-00860153/file/Sergent-et-al-incl-figures-accepted.pdf
DOI : 10.1016/j.fct.2013.07.061

Éditeur(s) : HAL CCSD
Résumé : International audience
Le jeu entre opinion et savoir et le moyen d'établir une connaissance objective sont au coeur des débats depuis l'Antiquité, et ont suscité, dans l'histoire des sciences, divers discours de la méthode. Ces thèmes ont également leur résonnance dans l'enseignement des sciences, et sont au coeur des travaux que nous avons menés sur la formation de l'esprit scientifique 1 qui, ne se limitant pas aux sciences, montrent par exemple que de nombreux élèves proposent de s'en remettre aux sondages ou aux opinions de proches sur des problèmes qui pourraient être tranchés par l'expérience, ou encore n'hésitent pas à affirmer leurs certitudes sans pour autant savoir. Selon le philosophe Karl Popper et le neurophysiologiste et prix Nobel John Eccles, la nature appartient au monde 1, les opinions subjectives au monde 2 et la connaissance objective au monde 3. C'est, pour Karl Popper, comme le résume Jacques Monod, une sélection darwinienne qui opère sur la diversité des idées, description évolutionniste de la recherche scientifique que l'enseignant peut adapter en classe, s'il souhaite là aussi emprunter une voie qui conduise du monde 2 au monde 3 : des opinions au savoir. Les travaux effectués avec une équipe d'enseignants de SVT engagent les élèves dans une telle voie, qui fragilise les opinions : le crédit qui est accordé à celles qui toujours surgissent, les siennes comme celles d'autrui, peut alors être interrogé.
ISSN: 2110-6460

Droits : info:eu-repo/semantics/OpenAccess
hal-01535160
https://hal.univ-antilles.fr/hal-01535160
https://hal.univ-antilles.fr/hal-01535160/document
https://hal.univ-antilles.fr/hal-01535160/file/Cariou_RDSTn%C2%B01_2010_Opinions%20vuln%C3%A9rables.pdf
DOI : 10.4000/rdst.163

Éditeur(s) : HAL CCSD Springer Verlag (Germany)
Résumé : International audience
A hybrid linear ion-trap Fourier-transform ion cyclotron resonance mass spectrometer was used for top-down characterization of the abundant human salivary Cystatins, including S, S1, S2, SA, SN, C, and D, using collisionally activated dissociation (CAD) after chromatographic purification of the native, disulfide intact proteins from saliva. Post-translational modifications and protein sequence polymorphisms arising from single nucleotide polymorphisms (SNPs) were assigned from precursor and product ion masses at a tolerance of 10 ppm allowing confident identification of individual intact mass tags. Cystatins S, S1, S2, SA and SN were cleaved of a N-terminal 20 amino-acid signal peptide, and Cystatin C a 26-residue peptide, to yield a generally conserved N-terminus. In contrast, Cystatin D isoforms with 24 and 28 amino-acid residue N-terminal truncations were found such that their N-termini were not conserved. Cystatin S1 was phosphorylated at Ser3, while S2 was phosphorylated at Ser1 and Ser3 of the mature protein, in agreement with previous work. Both Cystatin D isoforms carried the polymorphism C46R (SNP: rs1799841). The 14328 Da isoform of Cystatin SN previously assigned with polymorphism P31L due to a SNP (rs2070856) was found only in whole saliva. Parotid secretions contained no detectable Cystatins while whole saliva largely mirrored the contents of submandibular/sublingual (SMSL) secretions. Top-down high-resolution mass spectrometry is a powerful tool for the identification and characterization of potential protein biomarkers in saliva.
ISSN: 1044-0305

hal-00677483
https://hal.archives-ouvertes.fr/hal-00677483
DOI : 10.1016/j.jasms.2010.01.025

Éditeur(s) : HAL CCSD
Résumé : National audience
Nocardiosis is an infectious disease with wide range of clinical features, which can eventually lead to death. The agent responsible belongs to the genus Nocardia that includes about fifty different species. Nocardiosis occurs mainly in immunocompromised hosts. We report here three cases of disseminated nocardiosis misdiagnosed initially as cerebral metastatic lung cancer. These patients, including two immunocompetent hosts, presented with both pulmonary and cerebral lesions. In all three patients, the diagnosis was based on magnetic resonance imaging with diffusion sequence, apparent diffusion coefficient reconstruction and neurosurgical cerebral biopsies. Treatment with an appropriate antibiotic regimen was prolonged for several months. Progress was favorable with full resolution of the neurological symptoms and the radiological abnormalities. These three cases emphasize the diagnostic challenge of nocardiosis, especially in disseminated disease.
Revue Des Maladies Respiratoires

hal-01659440
https://hal-univ-rennes1.archives-ouvertes.fr/hal-01659440
DOI : 10.1016/j.rmr.2017.08.002
PUBMED : 28918971

Éditeur(s) : HAL CCSD Nature Publishing Group
Résumé : International audience
Epithelial-to-mesenchymal transition (EMT) promotes cell motility, which is important for the metastasis of malignant cells, and blocks CD95-mediated apoptotic signaling triggered by immune cells and chemotherapeutic regimens. CD95L, the cognate ligand of CD95, can be cleaved by metalloproteases and released as a soluble molecule (cl-CD95L). Unlike transmembrane CD95L, cl-CD95L does not induce apoptosis but triggers cell motility. Electron paramagnetic resonance was used to show that EMT and cl-CD95L treatment both led to augmentation of plasma membrane fluidity that was instrumental in inducing cell migration. Compaction of the plasma membrane is modulated, among other factors, by the ratio of certain lipids such as sphingolipids in the membrane. An integrative analysis of gene expression in NCI tumor cell lines revealed that expression of ceramide synthase-6 (CerS6) decreased during EMT. Furthermore, pharmacological and genetic approaches established that modulation of CerS6 expression/activity in cancer cells altered the level of C16-ceramide, which in turn influenced plasma membrane fluidity and cell motility. Therefore, this study identifies CerS6 as a novel EMT-regulated gene that has a pivotal role in the regulation of cell migration.Oncogene advance online publication, 17 March 2014; doi:10.1038/onc.2014.55.
ISSN: 0950-9232

hal-01063558
https://hal.archives-ouvertes.fr/hal-01063558
https://hal.archives-ouvertes.fr/hal-01063558/file/Down-regulation%20of%20ceramide_accepted.pdf
DOI : 10.1038/onc.2014.55
PUBMED : 24632610

Éditeur(s) : HAL CCSD Society of Exploration Geophysicists
Résumé : Read More: http://library.seg.org/doi/10.1190/geo2015-0411.1
International audience
Understanding the role of the unsaturated zone in aquifer recharge and contaminant attenuation processes is a major challenge for the protection and management of karstic water resources. We present the potential of the magnetic resonance soundings (MRS) geophysical method for characterizing the vadose zone of karst aquifers composed of epikarst and infiltration layers. To investigate the hydraulic functioning of the Durzon karst system located on the Larzac plateau (southern France), we used the MRS method at 16 sites. The MRS results have been compared with available geologic information and to core water content measurements. The remarkable spatial variability of the MRS response observed in the study area makes it possible to determine ranges of water storage properties in relation to the lithology of the investigated carbonate formations (dolomite, marly, and siliceous limestone). All soundings found either constant or increasing MRS water content with depth, which demonstrates that the infiltration zone might be the major water storage entity for permanent water storage, with important consequences for recharge quality and quantity. These results show the feasibility and potential of the MRS method for the characterization of the karst unsaturated zone and for understanding the vertical distribution of water content, which impacts the overall functioning of karst systems.Read More: http://library.seg.org/doi/10.1190/geo2015-0411.1
ISSN: 0016-8033

hal-01412654
https://hal.archives-ouvertes.fr/hal-01412654
https://hal.archives-ouvertes.fr/hal-01412654/document
https://hal.archives-ouvertes.fr/hal-01412654/file/mazilli2016.pdf
DOI : 10.1190/GEO2015-0411.1

Éditeur(s) : HAL CCSD Taylor & Francis
Résumé : International audience
Humans may be exposed via their environment to multiple chemicals as a consequence of human activities and use of synthetic products. Little knowledge is routinely generated on the hazards of these chemical mixtures. The metabolomic approach is widely used to identify metabolic pathways modified by diseases, drugs, or exposures to toxicants. This review, based on the state of the art of the current applications of metabolomics in environmental health, attempts to determine whether metabolomics might constitute an original approach to the study of associations between multiple, low-dose environmental exposures in humans. Studying the biochemical consequences of complex environmental exposures is a challenge demanding the development of careful experimental and epidemiological designs, in order to take into account possible confounders associated with the high level of interindividual variability induced by different lifestyles. The choices of populations studied, sampling and storage procedures, statistical tools used, and system biology need to be considered. Suggestions for improved experimental and epidemiological designs are described. Evidence indicates that metabolomics may be a powerful tool in environmental health in the identification of both complex exposure biomarkers directly in human populations and modified metabolic pathways, in an attempt to improve understanding the underlying environmental causes of diseases. Nevertheless, the validity of biomarkers and relevancy of animal-to-human extrapolation remain key challenges that need to be properly explored.
ISSN: 1093-7404

hal-00976116
https://hal-univ-rennes1.archives-ouvertes.fr/hal-00976116
https://hal-univ-rennes1.archives-ouvertes.fr/hal-00976116/document
https://hal-univ-rennes1.archives-ouvertes.fr/hal-00976116/file/Potential_input_from_metabolomics_accepted.pdf
DOI : 10.1080/10937404.2013.860318