Éditeur(s) : Impr. nationale Impr. nationale ( Port-au-Prince )
Résumé : (Dates or Sequential Designation) Began in 1856?
(Dates or Sequential Designation) -exercice 1948/1949.
(Numbering Peculiarities) Some years issued in parts.
At head of title, 18<89> 19 : République d'Haïti.
1891 has imprint: Paris : Marchal et Billard.
Haiti
Haïti

648674465

Éditeur(s) : HAL CCSD Public Library of Science
Résumé : International audience
Little information exists on the effects of ocean acidification (OA) on the digestive and post-digestive processes in marine fish. Here, we investigated OA impacts (Δ pH = 0.5) on the trophic transfer of select trace elements in the clownfish Amphiprion ocellaris using radiotracer techniques. Assimilation efficiencies of three essential elements (Co, Mn and Zn) as well as their other short-term and long-term kinetic parameters in juvenile clownfish were not affected by this experimental pH change. In complement, their stomach pH during digestion were not affected by the variation in seawater pH. Such observations suggest that OA impacts do not affect element assimilation in these fish. This apparent pCO2 tolerance may imply that clownfish have the ability to self-regulate pH shifts in their digestive tract, or that they can metabolically accommodate such shifts. Such results are important to accurately assess future OA impacts on diverse marine biota, as such impacts are highly species specific, complex, and may be modulated by species-specific metabolic processes.
ISSN: 1932-6203

hal-01564009
https://hal-univ-perp.archives-ouvertes.fr/hal-01564009
https://hal-univ-perp.archives-ouvertes.fr/hal-01564009/document
https://hal-univ-perp.archives-ouvertes.fr/hal-01564009/file/trophic%20transfer.pdf
DOI : 10.1371/journal.pone.0174344

Éditeur(s) : Elsevier
Résumé : Marine fish larvae undergo major morphological and cellular changes during the first month of life. The ontogeny of the gastrointestinal tract combines these two aspects of the larval development and is very interesting in that the timing of functional changes appears genetically hard-wired. The goal of this paper is to give an overview of the gastrointestinal development process in marine fish larvae, with particular attention to three species: sea bass; red drum; and sole, since the description of gut maturation in fish larvae was initiated during the last decade with these species. During the early stages, marine fish larvae exhibit particular digestive features. Concerning the exocrine pancreas, amylase expression decreases with age from the third week post-hatching in sea bass and red drum (approximately 400 degree days), whereas expression of other enzymes (trypsin, lipase, phospholipase A2...) increases until the end of the larva period. Moreover, secretory function of the exocrine pancreas progressively develops and becomes efficient after the third week of life. Concerning the intestine, enzymes of the enterocyte cytosol (in particular peptidase) have higher activity in young larvae than in older. Approximately in the fourth week of post-hatching development in sea bass, red drum and sole larvae, the cytosolic activities dramatically decline concurrently with a sharp increase in membranous enzyme activities of the brush border, such as alkaline phosphatase, aminopeptidase N, maltase... This process characterises the normal maturation of enterocytes in developing fish larvae and also in other vertebrates' species. The establishment of an efficient brush border membrane digestion represents the adult mode of digestion of enterocytes. This paper also describes the role of diet on the development of the gastrointestinal tract. Indeed, the maturational process of digestive enzyme can be enhanced, stopped, or delayed depending on the composition of the diet.
Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology (1532-0456) (Elsevier), 2001-12 , Vol. 130 , N. 4 , P. 477-487

Droits : 2001 Elsevier Science Inc. All rights reserved
http://archimer.ifremer.fr/doc/2001/publication-650.pdf
DOI:10.1016/S1532-0456(01)00274-5
http://archimer.ifremer.fr/doc/00000/650/

Éditeur(s) : Impr. nationale Impr. nationale ( Port-au-Prince )
Résumé : (Dates or Sequential Designation) Began in 1856?
(Dates or Sequential Designation) -exercice 1948/1949.
(Numbering Peculiarities) Some years issued in parts.
At head of title, 18<89> 19 : République d'Haïti.
1891 has imprint: Paris : Marchal et Billard.
Haiti
Haïti

648674465

Éditeur(s) : HAL CCSD BioMed Central
Résumé : International audience
BACKGROUND: In Guadeloupe and Martinique, two French Overseas Departments, colorectal cancer (CRC) has become an essential public health issue. However, little is known about CRC characteristics and the p53 status in these populations, particularly in Guadeloupe, whereas certification of a cancer registry has been recently validated. METHODS: This was a descriptive retrospective study of 201 patients who, between 1995 and 2000, underwent surgery for CRC in the Guadeloupe Teaching Hospital (GlpeTH; 83 patients) and in the Martinique Teaching Hospital (MqueTH; 118 patients). The clinicopathological features and the p53 expression, evaluated with immunohistochemistry, were compared at the time of diagnosis. A relationship between these parameters and the p53 expression was also studied. Data were analysed, using the SPSS computer software version 17.0. RESULTS: No statistical difference was found between the two groups of patients regarding age (p = 0.60), percentage of young patients (<=50 years; p = 0.94)), sex (p = 0.47), histological type (p = 0.073) and tumour sites (p = 0.65), although the GlpeTH patients were diagnosed with more distal colon cancers (54.2%) than the Mque TH patients (47.4%). By contrast, a significant difference was found regarding the tumour grade (p < 0.0001), the pTNM stage (p = 0.045) and the pT stage (p < 0.0001). Regarding p53 expression, solely for the MqueTH patients, nuclear expression was associated with pTNM, the percentage of p53 negative tumours increasing with the progression of the pTNM stages (p = 0.029). CONCLUSIONS: For the first time, this study reveals discrepancies in clinicopathological features and in the p53 status between the two groups of patients. The GlpeTH patients were diagnosed with more moderated CRCs but with few CRCs at pTNM IV stage. By contrast, the MqueTH patients were diagnosed with more differentiated tumours, but with many more CRCs at pTNM IV stage. This paradox may be due to differences in tumour location (distal vs proximal), multiplicity of the genetic profiles of patients, or patients getting treatment elsewhere. Although our study is limited due to its small size, it emphasizes the originality of our results.
ISSN: 1472-6890

inserm-00971313
http://www.hal.inserm.fr/inserm-00971313
http://www.hal.inserm.fr/inserm-00971313/document
http://www.hal.inserm.fr/inserm-00971313/file/1472-6890-14-12.pdf
DOI : 10.1186/1472-6890-14-12

Éditeur(s) : HAL CCSD Histochemical Society
Résumé : International audience
The galectin-4 protein is mostly expressed in the digestive tract and is associated with lipid raft stabilization, protein apical trafficking, wound healing, and inflammation. While most mammalian species, including humans, have a single Lgals4 gene, some mice have two paralogues: Lgals4 and Lgals6. So far, their significant similarities have hindered the analysis of their respective expression and function. We took advantage of two antibodies that discriminate between the galectin-4 and galectin-6 proteins to document their patterns of expression in the normal and the dextran sodium sulfate (DSS)-damaged digestive tract in the mouse. In the normal digestive tract, their pattern of expression from tongue to colon is quite similar, which suggests functional redundancy. However, the presence of galectin-4, but not galectin-6, in the lamina propria of the DSS-damaged colon, its association with luminal colonic bacteria, and differences in subcellular localization of these proteins suggest that they also have distinct roles in the normal and the damaged mouse digestive tract. Our results provide a rare example of ancestral and derived functions evolving after tandem gene duplication.
ISSN: 0022-1554

hal-01544764
https://hal.archives-ouvertes.fr/hal-01544764
DOI : 10.1369/0022155413478612

Éditeur(s) : HAL CCSD Station Biologique
Résumé : International audience
From fish samplings (rotenone: 6 February 2002 & 13 May 2003; boxtraps: January 2008 to July 2009) in amangrove lagoon, the demographic structure, reproductive period and diet of Lophogobius cyprinoides were studied.Individual fish were measured (< 1 mm), weighed (± 0.01 g), and sexed by observing the urogenital papilla, sex-ratios andtotal length (TL)-standard length and length-mass relationships were calculated. Five individuals per size class (2 mm) wereused to study sexual maturity by macroscopic observation of gonad structure with a sexual maturity scale, and diet byqualitative and quantitative examination of digestive tract contents and calculation of the following indices: levels offullness and vacuity; occurrence, number, and point frequencies of various food items, and quality of nutrition. Growth isisometric and sexual dimorphism in favor of males (78 vs. 64 mm), demographic structure (18 ≤ TL ≤ 78 mm) and conditionevolved over time, with a distinct femininization of the population. Sexual inversion appeared from 46 mm onward, andtotal masculinization at 72 mm. Protogynous hermaphrodism is advantageous in a sedentary, subcryptobenthic species thatis reproductively active almost throughout the year. The species has an omnivorous diet specialized in copepods and plants
ISSN: 0007-9723

hal-00746079
https://hal.univ-antilles.fr/hal-00746079

Éditeur(s) : Elsevier
Résumé : Until recently, it was considered impossible to feed newly hatched marine fish species with a compound diet. Substituting a compound diet for live prey was performed several weeks after hatching, depending on the species. Compound diets were well ingested at the early stage but larvae died with a gut full of food, suggesting that larvae were unable to digest the compound diet. The hypothesis was that younger larvae have insufficient digestive enzymes to thrive on compound diets, and that exogenous enzymes, provided from live prey, are necessary for early stages.The organogenesis of marine fish larvae is not completely achieved at hatching and histological studies have revealed that the anatomy of the digestive tract undergoes developmental changes over some weeks. Nevertheless, biochemical studies over 20 years have shown that most of the digestive enzymes are present in young larvae. Recent studies have provided better understanding of digestion mechanisms in larvae and have led to proposed dietary compositions meeting larvae nutritional requirements. Pancreatic digestive enzymes are detected before mouth opening. Their synthesis is not induced by diet ingestion, but secretory mechanisms in the pancreas, and so enzymatic action, become efficient chronologically after those of synthesis. Inadequate diets can delay the onset of secretion mechanisms. The ratio of secreted enzymes to total enzymes indicates the nutritional value of the diet ingested by the larvae.At the intestinal level, cytosolic enzymes, which are peptidases, exhibit high activity in the early stages, suggesting a high capacity in larvae to digest protein hydrolysate. Indeed, larvae growth and survival is improved by the incorporation of a moderate concentration of peptide or hydrolysate in the diet. Peptidase activity abruptly decreases around day 25 in sea bass, concurrent with an increase in enzymes of the brush border membranes. This corresponds to a normal maturation process of enterocytes. Compound diets can slightly delay the onset of this maturation process, and inadequate diet can prevent it, leading to near death of the larvae. A proper onset of the maturation process has been associated with high larvae survival.The early developmental stage larvae exhibit high hydrolytic capacity, related to their weight. Enzyme activity pattern is age-dependent, but can be modulated by diet composition. Thus, larvae have the ability to digest and thrive on compound diet, if this diet is well adapted. Larvae have different specificities in digestion and nutritional requirements when compared to juveniles. Taking these specificities into consideration, recent research has led to the formulation of a compound diet that was well adapted for larvae from mouth opening, and could totally replace live prey.
Aquaculture (0044-8486) (Elsevier), 2001-08 , Vol. 200 , N. 1-2 , P. 161-180

Droits : 2001 Elsevier Science B.V. All rights reserved
http://archimer.ifremer.fr/doc/2001/publication-447.pdf
DOI:10.1016/S0044-8486(01)00699-8
http://archimer.ifremer.fr/doc/00000/447/

Éditeur(s) : HAL CCSD Elsevier
Résumé : International audience
Orogenic lherzolites exhibit a specific (although reproducible) platinum-group element signature characterized by slight positive deviations of Pd/Ir, Rh/Ir and Ru/Ir ratios from the canonical chondritic model. Such a signature was alternatively considered to be a compositional feature of the primitive upper mantle or resulting from sulfide melt addition by refertilisation reactions in the continental lithosphere. To shed additional light on this conundrum, the distribution of PGE in FON B 93, an unserpentinized, fertile (3.24% Al2O3) orogenic lherzolite (French Pyrenees) used as in-house standard by our group, has been studied to different scales, from the bulk rock to trace minerals. In addition to new ICPMS analyses after separation into Nis and Te coprecipitation, its whole-rock PGE concentrations were redetermined by three different laboratories using very precise ID-ICP-MS methods after digestion of the sample in Carius tube at T=250 degrees C and 320 degrees C or in high-pressure asher (HPA-S). The four methods produce reproducible Ru, Rh and Pd contents (7.1 +/- 0.18 ppb; 1.43 +/- 0.05 ppb; 7.1 +/- 0.30 ppb) whereas the non-ID NiS-fire assay method underestimates the Os and Ir concentrations by c.a. 10 and 15% compared to ID-ICP-MS analyses (4.40 +/- 0.07 and 4.00 +/- 0.17 ppb, respectively). Platinum was the most difficult to analyse. If performed on powder aliquots smaller than 3 g, the ID-ICP-MS analyses generate strong nugget effects while the non-ID NiS-fire assay method yields statistically lower (but highly reproducible) Pt concentrations (6.92 +/- 0.26 ppb). These features reflect the strong partitioning of Pt into trace phases that SEM and laser ablation-ICP-MS analyses on thin sections identify as both high-temperature Pt-Ir-Os alloys and Pt-(Pd) tellurides of likely subsolidus origin; ten to fifteen grains ranging in maximum dimension from a few micrometres to a few hundreds of nanometers, were identified by standard polished thin section. LA-ICP-MS data on base metal sulfides (25 grains analysed), coupled with the whole-rock S concentration (277 +/- 10 ppm) and modal composition of the BMS (90% pentlandite+accessory pyrite and secondary pyrrhotite + 10% chalcopyrite) allowed the contribution of the BMS phase to the PGE budget of FON B 93 to be estimated. Except Pt that exhibits a 95% deficit in the BMS phase, the PGE concentrations measured by ID-ICP-MS can be balanced by BMS while Al-spinel is a negligible contributor, accounting for less than 0.5% of the Ru budget. The occurrence of Pt in trace phases may bias the whole-rock Pt concentrations because 1) mechanical collection of Pt-rich trace phases remains problematic with the Nis button, and 2) Pt-Ir-Os alloys may be prone to digestion problems in conventional Carius tube procedures. Since they could be stable at mantle depth, such refractory alloys that contain Os and Ir may also have enhanced the heavy PGEs/light PGEs fractionation. Our observations likely pertain to orogenic lherzolites as a whole because BMS assemblages in these mantle rocks record evolution at low sulfur fugacity, which prevents Pt-Ir-Os alloy from entering the Mss in the mantle; moreover, at subsolidus temperature, pentlandite, the major BMS, cannot accommodate 0 valence state Pt.
ISSN: 0009-2541

hal-00411486
https://hal.archives-ouvertes.fr/hal-00411486
DOI : 10.1016/j.chemgeo.2007.06.030

Résumé : This report is presented by Chantal Cahu for obtaining accreditation to supervise research. Since 1981, her research activity has addressed the study of reproducers' nutrition and the young stages of development of marine organisms belonging to two different branch lines, crustaceans and fish. This work, performed in a base-lined research body, IFREMER, is placed within a more general programme of aquaculture development. Indeed, whatever species are at issue, the development of aquaculture in France, as at the global level, is based on the production of juveniles in hatcheries. My work has therefore more precisely the goal of acquiring knowledge about the nutritional needs, mechanisms of digestion and the development of these animals in order to improve the production of prawn post-larvae and fish alevin in the hatchery. This work took place in three main periods that appear in this report: - The study of reproducers' nutrition and its influence on the quality of the Penaeid prawn's eggs and larvae. My work has focused mainly on the effect of lipids and vitamins on the development of eggs and larvae. - The study of the ontogenesis of the digestive functions and the nutritional needs of sea fish larvae, with bass as the model. The goal was to determine the specific nutritional needs of the young stages by studying the implementation and regulation of pancreatic and intestinal digestive enzymes. - The study of the regulation of certain developmental genes by means of nutrients, so as to understand how these nutrients behave during ontogenesis, particularly during development of the skeleton.
Ce mémoire est présentée par Chantal Cahu pour pour l'obtention d'une habilitation a diriger des recherches. Depuis 1981, son activité de recherche concerne l'étude de la nutrition des reproducteurs et des jeunes stades de développement d'organismes marins appartenant à deux embranchements différents, les crustacés et les poissons. Ce travail réalisé dans un organisme de recherche finalisée, l'Ifremer, se situe dans un programme plus général de développement de l'aquaculture. En effet, quelles que soient les espèces en jeu, le développement de l'aquaculture en France comme au niveau mondial repose sur la production de juvéniles en écloserie. Mon travail a donc plus précisément pour objectif l'acquisition de connaissances sur les besoins nutritionnels, les mécanismes de digestion et le développement des ces animaux afin d'améliorer la production de post-larves de crevettes et d'alevins de poissons en écloserie. Ce travail s'est déroulé en trois grandes périodes qui apparaissent dans ce mémoire : - L'étude de la nutrition des reproducteurs et son influence sur la qualité des oeufs et des larves de crevettes Pénéides. Mon travail a essentiellement porté sur l'effet des lipides et des vitamines sur le développement des oeufs et des larves. - L'étude de l'ontogenèse des fonctions digestives et les besoins nutritionnels des larves de poissons marins, avec le bar comme modèle. Le but était de déterminer les besoins nutritionnels spécifiques des jeunes stades, en étudiant la mise en place et la régulation des enzymes digestives pancréatiques et intestinales. - L'étude de la régulation de certains gènes du développement par les nutriments, de façon à comprendre comment ces nutriments agissent lors de l'ontogenèse, notamment lors du développement du squelette.

Droits : info:eu-repo/semantics/openAccess
http://archimer.ifremer.fr/doc/2006/rapport-2086.pdf
http://archimer.ifremer.fr/doc/00000/2086/

Éditeur(s) : HAL CCSD Public Library of Science
Résumé : International audience
The giant bathymodioline mussels from vents have been studied as models to understand the adaptation of organisms to deep-sea chemosynthetic environments. These mussels are closely related to minute mussels associated to organic remains decaying on the deep-sea floor. Whereas biological data accumulate for the giant mussels, the small mussels remain poorly studied. Despite this lack of data for species living on organic remains it has been hypothesized that during evolution, contrary to their relatives from vents or seeps, they did not acquire highly specialized biological features. We aim at testing this hypothesis by providing new biological data for species associated with organic falls. Within Bathymodiolinae a close phylogenetic relationship was revealed between the Bathymodiolus sensu stricto lineage (i.e. ``thermophilus'' lineage) which includes exclusively vent and seep species, and a diversified lineage of small mussels, attributed to the genus Idas, that includes mostly species from organic falls. We selected Idas iwaotakii (Habe, 1958) from this latter lineage to analyse population structure and to document biological features. Mitochondrial and nuclear markers reveal a north-south genetic structure at an oceanic scale in the Western Pacific but no structure was revealed at a regional scale or as correlated with the kind of substrate or depth. The morphology of larval shells suggests substantial dispersal abilities. Nutritional features were assessed by examining bacterial diversity coupled by a microscopic analysis of the digestive tract. Molecular data demonstrated the presence of sulphur-oxidizing bacteria resembling those identified in other Bathymodiolinae. In contrast with most Bathymodiolus s.s. species the digestive tract of I. iwaotakii is not reduced. Combining data from literature with the present data shows that most of the important biological features are shared between Bathymodiolus s.s. species and its sister-lineage. However Bathymodiolus s.s. species are ecologically more restricted and also display a lower species richness than Idas species.
ISSN: 1932-6203

hal-01545914
https://hal.archives-ouvertes.fr/hal-01545914
DOI : 10.1371/journal.pone.0069680

Éditeur(s) : Actes de colloques Ifremer, Tahiti, French Polynesia, 20 Feb - 4 Mar 1989, n°9, chap. 68, pp.751-755
Résumé : If warmwater finfish protein requirements as level in diet is low, absolute intake per day is similar to those of coldwater species. But relation of this requirement with specific growth rate differs for strictly warmwater fishes as Tilapias. Analysis of partition between protein and non-protein energy retention shows that these species with Clarias make a better use of non-protein energy provided and consequently improve their protein retention. Better carbohydrate digestibility and metabolism are propounded as part of the explanation.

Droits : info:eu-repo/semantics/openAccess
http://archimer.ifremer.fr/doc/1989/acte-1424.pdf
http://archimer.ifremer.fr/doc/00000/1424/

Éditeur(s) : Co-tutelle Al Centro de Investigaciones Biológicas del Noroeste - Université de Bretagne occidentale
Résumé : The interest in probiotics for aquaculture is increasing rapidly. In the present work we evaluated the potential of some yeast to induce the larvae gut maturation in sea bass larvae Dicentrarchus labrax. The production of polyamines putrescine, spermidine and spermine was quantified in ten yeast strains using high performance liquid chromatography (HPLC). Afterwards, fluorescently labelled yeast adhesion to the intestine of sea bass larvae and the spotted sand bass (Paralabrax maculatofasciatus) was evaluated by fluorescent microscopy. Two of them, CBS 8339 strain (Debaryomyces hansenii) and X2180 strain (Saccharomyces cerevisiae) produce the polyamines of our interest. The same strains showed the best adhering capabilities to the sea bass and spotted sand bass intestine. Both strains were selected and introduced by pulverization into the diet of larvae. The larvae fed with CBS 8339 strain, increase survival in 8.3% but not in their weight. The secretion of trypsin and amylase as well as those from the brush border membrane: alkaline phosphatase, maltase and leucine aminopeptidase, are incresed at 27 days after hatching (dah). At 42 dah difference in the enzyme activity of the different groups does not exist, suggesting a maturation of the digestive system in early stages of larval development. A second experiment was carried out to know the effect of different levels of incorporation into the diet of CBS 8339 strain: 1.1% and 5.7%. The minimum level, 1.1% of yeast produce best growth and survival rates, as well as lower deformed larvae than those obtained by 5.7% and the control one. Both level of yeast inclusion favored the trypsin secretion and lipase activity at 26 dah. Whereas mRNA levels for trypsin and amylase were influenced by both yeast concentrations at 26 and 36 dah, which suggest a development of digestive capacity of larvae as a result of the presence of polyamines secreted by the yeast.
Les probiotiques ont été définis comme des préparations microbiennes vivantes utilisées comme additif alimentaire, et qui ont une action bénéfique sur l'animal hôte en améliorant la digestion et l'hygiène intestinale. L'application de tels traitements aux productions aquacoles suscite un intérêt qui s'accroît rapidement. Dans ce travail, nous avons étudié l'effet de souches de levures sur la maturation de l'intestin des larves du bar Dicentrarchus labrax. Ces souches produisent des polyamines qui sont impliquées dans la réplication et la différentiation cellulaire, ainsi que dans la synthèse des protéines et des acides nucléiques. Dix souches de levures ont été testées pour leur production de polyamines (putrescine, spermidine et spermine), et pour leur adhérence à la paroi intestinale du bar et la cabrilla (Paralabrax maculatofasciatus). La production des polyamines a été analysée en utilisant la chromatographie liquide à haute performance (HPLC). Les levures selectionées ont été marquées avec un composé fluorescent pour tester leur adhérence a l'intestin de larves de bar et de juvéniles de cabrilla. L'adhérence a pu ainsi être evaluée par observation microscopique en épifluorescence. Les souches CBS 8339 (Debaryomyces hansenii) et X2180 (Saccharomyces cerevisiae) ont été choisies pour leur forte production de spermidine et de spermine, respectivement, et pour leur capacité d'adhérence à l'intestin du bar et de la cabrilla. Les deux souches ont été ajoutées par pulvérisation au régime des larves. Nous avons observé une augmentation de 8.3% du taux de survie avec les larves alimentées avec la souche CBS 8339, mais le poids moyen de ces larves était inférieur à celui obtenu sans ajout de levure. Une augmentation de l'activité de la trypsine et de l'amylase, aussi bien que des activités enzymatiques dans la bordure en brosse, aminopeptidase, phosphatase alkaline et maltase, ont été observées dans les larves âgées de 27 jours alimentées avec la souche CBS 8339. Au jour 42, il n'existait plus de différence d'activité enzymatique entre les larves traitées ou non avec la levure. Ces résultats suggèrent que la maturation du système digestif a été accélérée chez les larves nourries avec la levure, la maturation étant achevée chez les larves de 42 jours, qu'elles aient reçu l'un ou l'autre régime. Une deuxième expérience a été effectuée pour étudier l'effet de différents niveaux d'incorporation dans le régime contenant 1,1% et 5,7% de levure CBS 8339. L'incorporation de 1,1% a induit une augmentation du poids moyen et du taux de survie des larves, ainsi qu'une réduction du taux de malformation de la colonne vertébrale, par rapport aux résultats obtenus avec 0 ou 5,7% de levure. Les deux niveaux d'incorporation de la levure ont favorisé l'activité de la trypsine et de la lipase au jour 26. Les niveaux d'ARNm codant pour la synthèse de la trypsine et de l'amylase ont été influencés par les deux doses de levure aux jours 26 et 36. Ces résultats suggèrent qu'une faible dose de levure améliore le développement et la capacité digestive des larves, et nous formulons l'hypothèse que cette efficacité est due à la production de polyamines in situ. Le rôle des polyamines est particulièrement important pendant les phases de forte croissance comme les stades larvaires, mais elles peuvent être toxiques à dose trop élevée. L'incorporation de levure dans l'aliment destiné aux larves semble donc une excellente méthode pour délivrer les polyamines chez l'animal hôte.

Droits : info:eu-repo/semantics/openAccess
http://archimer.ifremer.fr/doc/2002/these-103.pdf
http://archimer.ifremer.fr/doc/00000/103/

Résumé : This Gambas report contains two parts. The summary report (Volume 1) gives an account of the project intervention from May 2000 to December 2003, and a digest of the project's results and deliverables. The comprehensive report (Volume 2) contains the details of the scientific research and the technical results brought about by the project. Besides, a CD/ROM containing all the technical reports, publications and maps generated in the frame of Gambas is available from the coordinator.

Droits : info:eu-repo/semantics/openAccess
http://archimer.ifremer.fr/doc/00083/19386/16980.pdf
http://archimer.ifremer.fr/doc/00083/19386/16997.pdf
http://archimer.ifremer.fr/doc/00083/19386/

Éditeur(s) : Elsevier
Résumé : A study was conducted using a bioenergetics approach to generate information on energy requirement and feed utilization of Litopenaeus stylirostris. Animals (initial mean weight 21 +/- 1 g were fed ad libitum six experimental diets, ranging from 25 to 58% crude protein (CP), for 50 days. Weight gain increased from 21 to 30 g with increasing dietary protein level. Survival rates averaged 80%. Basal metabolism (HeE) and heat increment of feeding (HiE) were monitored using respirometry. HeE was on average I U shrimp(-1) day(-1) or 47 U kg live weight(-1) day (22 kJ/kg(0.8). d(-1)), slightly more than what is observed in fish. HiE averaged 0.2 W/sbrimp(-1) day(-1) or 10 W kg live weight(-1) day(-1) (4 kJ/kg(0.8) d(-1)). It represented 3 1 % and 12% digestible energy intake (DET) for shrimp fed on 58% CP and 25% CP diet respectively. Non-fecal (UE + ZE) energy calculated on the basis of N-ammonia excretion averaged 0.2 jig N-ammonia/g dry wt./ mn or 25 J live shrimp(-1) day(-1) in fasting stage and increased to 40 J in post-prandial stage. Ammonia production increased with increasing dietary crude protein (CP). The O:N ratio indicated that protein was increasingly used as an energy substrate as CP increased. The information was used to construct an energy budget for shrimp fed a protypical 40% CP diet. Gross energy intake (IE) was estimated at 6.5 W live shrimp(-1) day(-1); digestible energy intake (DEI) at 5, urinary and branchial excretion (UE +ZE) at 1.2, total heat production (HE) at 3.2; recovered energy (RE) at 0.6 (or 11% DEI). L. stylirostris adults issued from domesticated strain appeared to be more efficiently utilizing (i.e. converting into carcass energy) protein than carbohydrates. This preliminary energy budget can be used to construct theoretical feed requirement and waste outputs model for L. stylirostris. (C) 2007 Published by Elsevier B.V.
Aquaculture (0044-8486) (Elsevier), 2007-10 , Vol. 271 , N. 1-4 , P. 439-448

Droits : 2007 Published by Elsevier B.V.
http://archimer.ifremer.fr/doc/2007/publication-6263.pdf
DOI:10.1016/j.aquaculture.2006.05.062
http://archimer.ifremer.fr/doc/00000/6263/

Éditeur(s) : HAL CCSD Public Library of Science
Résumé : International audience
BACKGROUND: Gastric cancer is one of the most common cancers in the world. The "economically developed countries" life style, including diet, constitutes a risk factor favoring this cancer. Diet modulation may lower digestive cancer incidence. Among promising food components, dairy propionibacteria were shown to trigger apoptosis of human colon cancer cells, via the release of short-chain fatty acids acetate and propionate. METHODOLOGY/PRINCIPAL FINDINGS: A fermented milk, exclusively fermented by P. freudenreichii, was recently designed. In this work, the pro-apoptotic potential of this new fermented milk was demonstrated on HGT-1 human gastric cancer cells. Fermented milk supernatant induced typical features of apoptosis including chromatin condensation, formation of apoptotic bodies, DNA laddering, cell cycle arrest and emergence of a subG1 population, phosphatidylserine exposure at the plasma membrane outer leaflet, reactive oxygen species accumulation, mitochondrial transmembrane potential disruption, caspase activation and cytochrome c release. Remarkably, this new fermented milk containing P. freudenreichii enhanced the cytotoxicity of camptothecin, a drug used in gastric cancer chemotherapy. CONCLUSIONS/SIGNIFICANCE: Such new probiotic fermented milk may thus be useful as part of a preventive diet designed to prevent gastric cancer and/or as a food supplement to potentiate cancer therapeutic treatments.
ISSN: 1932-6203

inserm-00871491
http://www.hal.inserm.fr/inserm-00871491
http://www.hal.inserm.fr/inserm-00871491/document
http://www.hal.inserm.fr/inserm-00871491/file/Milk_Fermented_by_Propionibacterium_freudenreichii.accepted.pdf
DOI : 10.1371/journal.pone.0031892

Résumé : Les systèmes d'élevage des petits ruminants au pâturage sont les plus répandus en zone tropicale humide mais l'exposition aux strongles gastro-intestinaux entraîne des pertes importantes de production. L'objectif de ce travail est d'évaluer un système de gestion mixte du pâturage associant diverses espèces animales permettant à priori d'accroître les performances via la réduction du parasitisme et!ou une meilleure alimentation. L'étude de la littérature a conduit à une méta-analyse confirmant de meilleures performances individuelles ou à l'hectare pour les ovins en mixte. Préalablement un dispositif visant à vérifier la non-transmission des larves d'Haemonchus contortus des caprinsaux génisses Créoles a été validé. Un second dispositif avec des chevrettes conduites en mixte, infestées ou pas par Haemonchus comortus (Ml et MnI) ont été comparées à des chevrettes contrôles infestées ou non (CI et CnI). Ce dispositif a été conduit pendant 2 ans avec des mesures du couvert pâturé, des mesures individuelles des quantités ingérées, de la digestibilité, du parasitisme et de la croissance. De plus fortes croissances ont étéobservées en pâturage mixte que les chevrettes soient infestées ou pas (43.25 et 31.68 glj pour MDI et MI vs. 32.44 et 17.91 glj) avec une moindre biomasse. Les mesures d'ingestion et d'ingéré digéré ont été corrélées aux croissances des chevrettes contrairement aux variables parasitaires et met en évidence le rôle de l'alimentation et de la résilience dans le bénéfice du pâturage mixte. Une autre gestion intégrée du pâturage a été étudiée basée sur l'utilisation du vermicompost et s' ant sur les mêmes leviers d'action: l'alimentation et le itisme
Systems of small ruminant grazing are most prevalent in the humid tropics but this environment exposes animals to gastro-intestinal with production losses. The objective ofthis thesis is to evaluate a system ofmixed management ofthe pasture combining various animal species, which allows a priori to increase animal performance by reducing the parasitism and/or better nutrition. The study of literature bas led to a meta-analysis confirming the better individual performances or calculated per ha for sheep reared mixed. The first experiment validated the non-transmission of Iarvae ofHaemonchus contortus from goats to heifers. In a second experiment, goats mixed with heifers, infested or not with Haemonchus contortus (Ml vs MnI) were compared with controls goats, reared alone, infested or not (CI and CNI). This deviee with a continuous driving was studied for two years with measurements on the sward, individual measures of intake, digestibility, parasitism and growth ofthe goats. The highest growth rates were measured with the mixed pasture as goats are infested or not (43.25 and 31.68 g / d for MN! and MI vs. 32.44 and 17,91 g / d) with lower biomass. The herbage intake and the digested herbage intake were well correlated to the growth rate, unIike parasitic variables, and highlight the role of diet in the benefit of mixed driving for goats. Therefore, the infested goats with Haemonchus, expressed greater resilience with improved growth performance compared with controls. Parallel to the study ofmixed pasture, another integrated management of grazing was studied, based on the use of vermicompost and relying on the same levers. namelv throuzh feedinz and parasitism

http://www.theses.fr/2012AGUY0537/document

Éditeur(s) : HAL CCSD BioMed Central
Résumé : International audience
BACKGROUND:In French Guiana, a French overseas territory in South America, 6 to 10 thousands undocumented persons work illegally in gold mining sites in the Amazonian forest. Precarious life conditions lead to poor health but few data exist on the health status of illegal gold miners in French Guiana. The objective of this article was to describe the sociodemographic and health status of this vulnerable population.METHOD:A prospective cross-sectional survey was conducted in 2015 on gold mine supply sites at the border between French Guiana and Suriname. Health status was assessed through medical examination, past medical history, haemoglobin concentration, and HIV and malaria testing. A questionnaire was used to collect data about the migration itinerary and life conditions on mining sites.RESULTS:Among the 421 adults included in the study, 93.8% (395/421) were Brazilian, mainly from Maranhão (55.7%, 220/395), the poorest Brazilian state. The sex ratio was 2.4. Overall, 48% of persons never went to school or beyond the primary level. The median time spent in gold mining was quite long (10 years), with a high turn-over. One third of the surveyed population (37.1%, 156/421) had high blood pressure, and only two had a medical follow-up. Most persons had experienced malaria (89.3%, 376/421). They declared frequent arboviroses and digestive disorders. Active leishmaniasis was observed in 8.3% of gold miners. Among women, 28.5% were anemic. Concerning HIV, 36.6% (154/421) of persons, mainly men, never got tested before and 6 were tested positive, which represented an HIV prevalence of 1.43% (95%CI =0.29-2.5).CONCLUSION:These findings support the hypothesis that mining in remote areas is linked to several specific illnesses. Theoretically, gold miners would be presumed to start their economical migration to French Guiana as a healthy group. However, their strenuous working and living conditions there lead to poor health caused by infectious and non infectious diseases. This description of their health status is precious for health policy planners in French Guiana given the importance of controlling communicable disease, and the severity and range of specific illnesses acquired by this neglected population.TRIAL REGISTRATION:Clinical trial registration PRS N° NCT02903706 . Retrospectively registered 09/13/2016.
ISSN: 1471-2458

inserm-01566314
http://www.hal.inserm.fr/inserm-01566314
http://www.hal.inserm.fr/inserm-01566314/document
http://www.hal.inserm.fr/inserm-01566314/file/2017%2C%20Douine%20-%20Illegal%20gold%20miners%20in%20French%20Guiana.pdf
DOI : 10.1016/S0140-6736(10)62169-1

Éditeur(s) : HAL CCSD Oxford University Press (OUP)
Résumé : International audience
STUDY QUESTION: Can protein biomarkers of the male genital tract be identified in human seminal plasma? SUMMARY ANSWER: We identified potential biomarkers for each of the organs participating in the secretions of the human seminal plasma. WHAT IS KNOWN ALREADY: The seminal plasma fulfills critical functions for fertility by providing spermatozoa with a protective milieu, promoting their final maturation and modulating the immune responsiveness of the female reproductive tract. It is also considered to be a promising source of biomarkers of male infertility and/or pathologies of the male genital tract. STUDY DESIGN, SIZE, DURATION: This study combines proteomic analyses of normal seminal plasma together with transcriptomic gene expression profiling of human healthy tissues. MATERIALS, SETTING, METHODS: Non-liquefied seminal plasma proteins from a healthy donor were prefractionated using two sequential Proteominer™ libraries. Eight subproteome fractions were collected, trypsin digested and subjected to three successive mass spectrometry analyses for peptide characterization. The list of identified proteins was compared with and merged with other available data sets of the human seminal plasma proteome. The expression of corresponding genes was then investigated using tissue transcriptome profiles to determine where, along the male reproductive tract, these proteins were produced. Finally, tissue specificity of a selected subset of biomarker candidates was validated on human tissues. MAIN RESULTS AND THE ROLE OF CHANCE: We first performed a proteomic analysis of the human seminal plasma and identified 699 proteins. By comparing our protein list with other previous proteomic data sets, we found that 2545 unique proteins have been described so far in the human seminal plasma. We then profiled their expression at the gene level and identified 83 testis, 42 epididymis, 7 seminal vesicle and 17 prostate candidate protein markers. For a subset of testis-specific candidates, i.e. TKTL1, LDHC and PGK2, we further validated their germ cell expression and demonstrated that such markers could distinguish between semen from fertile and infertile men. LIMITATIONS, REASONS FOR CAUTION: While some of the markers we identified are well-known tissue-specific products, further dedicated studies to validate the biomarker status of new candidates will be required. Additionally, whether or not the abundance of these proteins is indeed decreased in some specific pathological situations remains to be determined. WIDER IMPLICATIONS OF THE FINDINGS: Using an integrative genomics approach, we identified biomarker candidates for each of the organs participating in the seminal plasma production. In this study, we essentially focused on germ cell markers and their potential application for the diagnosis of male infertility. Other types of markers also deserve a focused attention given their potential predictive value for various reproductive disorders, notably for prostate cancers. STUDY FUNDING/COMPETING INTEREST(S): This work was supported by the Proteomics Core Facility at Biogenouest and was funded by Conseil Régional de Bretagne, IBiSA and Agence de la Biomédecine grants. The authors declare that there exists a competing financial interest in this work that is related to a patent application on the use of identified germ cell-specific proteins in an antibody-based assay (Fertichip™) to predict the successful testicular biopsy outcomes in human non-obstructive azoospermia.
ISSN: 0268-1161

inserm-00814146
http://www.hal.inserm.fr/inserm-00814146
DOI : 10.1093/humrep/des360
PUBMED : 23024119

Éditeur(s) : HAL CCSD American Chemical Society
Résumé : International audience
Cyanobacterial toxins and pesticides regularly impact freshwaters. Microcystin-LR is one of the most toxic and common cyanobacterial toxins whereas glyphosate is the active ingredient of a widely use herbicide. As filter feeders, freshwater mussels are particularly exposed. Like many native bivalve species, Unio pictorum suffers from a continuous decline in Europe. In order to get a deeper insight of its response to contaminants, U. pictorum was exposed to either 10 μg L(-1) of microcystin-LR or 10 μg L(-1) of glyphosate or a mixture of both. Proteins of the digestive glands were extracted and analyzed by DIGE. Gel analysis revealed 103 spots with statistical variations, and the response seems to be less toward glyphosate than to microcystin-LR. Specific spots have variations only when exposed to the mixture, showing that there is an interaction of both contaminants in the responses triggered. The proteins of 30 spots have been identified. They belong mostly to the cytoskeleton family, but proteins of the oxidative pathway, detoxification, and energetic metabolism were affected either by glyphosate or microcystin-LR or by the mixture. These results demonstrate the importance to study contaminants at low concentrations representative of those found in the field and that multicontaminations can lead to different response pathways.
ISSN: 1535-3893

hal-00877711
https://hal.archives-ouvertes.fr/hal-00877711
DOI : 10.1021/pr4006316
PUBMED : 23972258