Éditeur(s) : HAL CCSD Mineralogical Society of America
Résumé : International audience
In situ mineralization of CO2 in ultramafic rock-hosted aquifers is one of the promising solutions for decreasing CO2 concentrations in the atmosphere. Naturally altered ultramafic rocks suggest that carbonation processes are controlled by local heterogeneities in the structure of the rock and fluid transport at the water-rock interfaces. We studied the role of rock crystallographic anisotropy relative to the global fluid flow direction on the mineralization of CO2 by means of electron microscope analyses from the macro- to the micrometer scale (EBSD-FIB). The sample used for the measurements was a hot pressed olivine core percolated by water enriched in CO2 (pCO2 = 10 MPa) at 180 °C. During the percolation experiment, olivine was dissolved and two types of carbonates, dolomite, and magnesite, were precipitated on olivine surfaces. The results showed that the dissolution of olivine is controlled by its crystallographic properties as shown by the development of etch-pits only on the (010)ol planes and with elongated shapes parallel to the [010]ol axes. In contrast, the precipitation of carbonates is governed by hydrodynamic properties. Carbonates are heterogeneously distributed in the percolated rock. They are mainly located along the moderate (for dolomite) and the minor (for magnesite) flow paths, both oriented parallel to the principal fluid flow direction, which allow carbonates to be supplied with divalent cations (e.g., Ca2+, Mg2+, and Fe2+). In these flow paths, carbonate growth is systematically oriented normal to the flow that facilitates the development of chemical gradients with cationic supersaturation conditions for carbonate precipitation near the walls.In natural systems, the (010)ol planes are parallel to the Moho and the (100)ol planes are vertical; our study suggests that flow of CO2-rich fluids will induce precipitation of carbonates localized along, and preferentially clogging, vertical flow paths while favoring olivine dissolution along horizontal fluid pathways. This dual control of structure and fluid flow on carbonation mechanisms could be an important parameter allowing sustainable CO2 storage in peridotites, while limiting the risks of leakage toward the surface.
ISSN: 0003-004X

hal-01143393
https://hal.archives-ouvertes.fr/hal-01143393
DOI : 10.2138/am-2015-4913

Éditeur(s) : HAL CCSD Elsevier
Résumé : International audience
Carbonation of peridotite is a widespread process in nature, with emerging societal and environmental implications through geological storage of CO2. We studied the micro- and nano-scale structures associated with the carbonation of peridotite pervaded by a CO2-rich water and the induced changes of its hydrodynamic properties. Focused ion beam (FIB) nanotomography was coupled with scanning electron microscopy (SEM) and transmission electron microscopy (TEM) observations. An experimental sample of dunite core percolated by a CO2-rich fluid (pCO2 = 11 MPa) at 160 °C was compared with a naturally carbonated sample from the Atlantic Ridge (Exp. IODP 304). Results of the 8-days percolation experiment show continuous alteration of olivine into magnesite locally associated with a poorly crystalline serpentine-type mineral referred to as proto-serpentine. The experiment is also characterized by an overall decrease in rock porosity (from 7.7 to 6.4%) and permeability (from 8.5 × 10− 16 to 1.7 × 10− 16 m2). The primary inter-granular porosity (1 to 10 μm) is progressively filled with magnesite, leading to the overall decrease of permeability. At the same time, a secondary small-scale porosity (10 nm to 1 μm) is created at the tip of the dissolution features along the (010) cleavage planes of olivine grains. Carbonate crystals grow within these etch-pit structures behind the dissolution front. Dissolution and precipitation occur at a relative rate that maintains the secondary porosity over the timescale of the experiment (i.e. carbonate growth has to be the slowest one). This process is interpreted as an interface coupled dissolution-precipitation mechanism of olivine carbonation. Proto-serpentine is only formed in highly reduced flow areas, such as small olivine etch-pits (10 to 500 nm wide), which are more isolated from CO2 input. SEM-FIB analyses show the 3D preferential orientation of the dissolution-precipitation planes within a given olivine grain. It suggests the formation of lateral connections between the etch-pits (infra-micrometric “channels”) providing access to reactants and removing reaction products at the reaction sites. The experimental results show that olivine dissolution and magnesite precipitation converge to a quasi-steady state resulting in a constant carbonation rate controlled by reaction kinetics and not by mass transfer for this flow regime. The system self-regulates, independently of the porosity change at least during this incipient stage of olivine carbonation. Similar textures are observed in natural samples collected at 170 m-depth in the vicinity of veinlets driving late fluids down into the cooling oceanic lithosphere. This suggests that similar mechanisms are active in nature and can play an important role in the late, low temperature alteration stages of fractured peridotite. These results also highlight the critical role of nm-scale spatial arrangement of dissolution and precipitation features on mass transfers and on the sustainability of olivine carbonation through coupled dissolution-precipitation processes.
ISSN: 0009-2541

hal-01684733
https://hal.archives-ouvertes.fr/hal-01684733
DOI : 10.1016/j.chemgeo.2017.11.013

Éditeur(s) : HAL CCSD Taylor & Francis
Résumé : International audience
Abstract Indoor fungal contamination has been associated with a wide range of adverse health effects, including infectious diseases, toxic effects and allergies. The diversity of fungi contributes to the complex role that they play in indoor environments and human diseases. Molds have a major impact on public health, and can cause different consequences in hospitals, homes and workplaces. This review presents the methods used to assess fungal contamination in these various environments, and discusses advantages and disadvantages for each method in consideration with different health risks. Air, dust and surface sampling strategies are compared, as well as the limits of various methods are used to detect and quantify fungal particles and fungal compounds. In addition to conventional microscopic and culture approaches, more recent chemical, immunoassay and polymerase chain reaction (PCR)-based methods are described. This article also identifies common needs for future multidisciplinary research and development projects in this field, with specific interests on viable fungi and fungal fragment detections. The determination of fungal load and the detection of species in environmental samples greatly depend on the strategy of sampling and analysis. Quantitative PCR was found useful to identify associations between specific fungi and common diseases. The next-generation sequencing methods may afford new perspectives in this area.
ISSN: 1040-841X

hal-00874670
https://hal.archives-ouvertes.fr/hal-00874670
DOI : 10.3109/1040841X.2013.777687
PUBMED : 23586944

Éditeur(s) : HAL CCSD Springer Verlag
Résumé : International audience
Transverse drying shrinkage was measured at microscopic and mesoscopic levels in poplar wood characterised by an increasing growth strain (GS), from normal to tension wood. Results show that: (a) The drying shrinkage, measured as a relative thickness decrease, was significantly higher for G-layer (GL) than the other layers (OL), GL shrinkage was not significantly correlated with GS and OL shrinkage was negatively correlated with GS. (b) In gelatinous fibre (G-fibre), lumen size increased during drying and this increase was positively related with GS, but in normal wood fibre lumen size decreased during drying. These findings suggest that GL shrank outwards, so that its shrinkage feebly affected the total cell shrinkage and the mesoscopic shrinkage was controlled by the OL shrinkage which shrank inwards. (c) Measurements done on 7×7 mm² thin sections evidenced a negative correlation between transverse shrinkage and GS, significant in T direction but weak in R direction. These observations at both levels allow to discuss the contribution of GL to the mesoscopic shrinkage of tension wood.
ISSN: 0043-7719

hal-00194930
https://hal.archives-ouvertes.fr/hal-00194930
https://hal.archives-ouvertes.fr/hal-00194930/document
https://hal.archives-ouvertes.fr/hal-00194930/file/WST_FANG2006.pdf
DOI : 10.1007/s00226-007-0148-3

Éditeur(s) : HAL CCSD Public Library of Science
Résumé : International audience
The giant bathymodioline mussels from vents have been studied as models to understand the adaptation of organisms to deep-sea chemosynthetic environments. These mussels are closely related to minute mussels associated to organic remains decaying on the deep-sea floor. Whereas biological data accumulate for the giant mussels, the small mussels remain poorly studied. Despite this lack of data for species living on organic remains it has been hypothesized that during evolution, contrary to their relatives from vents or seeps, they did not acquire highly specialized biological features. We aim at testing this hypothesis by providing new biological data for species associated with organic falls. Within Bathymodiolinae a close phylogenetic relationship was revealed between the Bathymodiolus sensu stricto lineage (i.e. ``thermophilus'' lineage) which includes exclusively vent and seep species, and a diversified lineage of small mussels, attributed to the genus Idas, that includes mostly species from organic falls. We selected Idas iwaotakii (Habe, 1958) from this latter lineage to analyse population structure and to document biological features. Mitochondrial and nuclear markers reveal a north-south genetic structure at an oceanic scale in the Western Pacific but no structure was revealed at a regional scale or as correlated with the kind of substrate or depth. The morphology of larval shells suggests substantial dispersal abilities. Nutritional features were assessed by examining bacterial diversity coupled by a microscopic analysis of the digestive tract. Molecular data demonstrated the presence of sulphur-oxidizing bacteria resembling those identified in other Bathymodiolinae. In contrast with most Bathymodiolus s.s. species the digestive tract of I. iwaotakii is not reduced. Combining data from literature with the present data shows that most of the important biological features are shared between Bathymodiolus s.s. species and its sister-lineage. However Bathymodiolus s.s. species are ecologically more restricted and also display a lower species richness than Idas species.
ISSN: 1932-6203

hal-01545914
https://hal.archives-ouvertes.fr/hal-01545914
DOI : 10.1371/journal.pone.0069680

Éditeur(s) : HAL CCSD Geological Society
Résumé : Gem opals from Wegel Tena, Wollo Province, Ethiopia, occur in Oligocene rhyolitic ignimbrites. They display a unique geochemistry, with some samples yielding the highest Ba concentrations ever recorded. They are generally much richer in chemical impurities than opals from other localities. For example, the sum Al+Fe or the sum Na+Mg+Ca+K+Ba are often higher. These geochemical features make them easy to distinguish from other opals worldwide. We observed strong geochemical variations and some good positive correlations in our samples, such as Al+Fe vs. Na+Mg+Ca+K+Ba, Al vs Ca, or Ba vs Ca. This shows that the crystallography of opal has controlled, at least in part, the incorporation of chemical impurities, although opal is not well-crystallized. In addition, the multimodal distributions of several chemical impurities (e.g. U vs Sr, Al vs Ca, Ba vs Ca, etc.) suggest at least two origins of silica: weathering of feldspars and weathering of volcanic glass. In addition, opals from Wegel Tena contain numerous well-preserved microscopic plant fossils. Moreover, their host rock exhibits features typical of pedogenesis (abundant clays, desiccation cracks, and grain size sorting). We propose that the opals at Wegel Tena formed during the Oligocene period when volcanic emissions stopped for a time long enough to allow weathering of ingimbrites and therefore liberation of silica. This accompanied the formation of soil and development of plant life, and some plants were trapped in opal.
ISSN: 1467-7873

hal-00767555
https://hal.archives-ouvertes.fr/hal-00767555
DOI : 10.1144/1467-7873/10-MINDEP-058

Éditeur(s) : HAL CCSD Wiley
Résumé : 13 pages, 4 figures, to be published on J. Am. Cer. Soc
International audience
Since crack propagation in oxide materials at low crack velocities is partly determined by chemical corrosion, proper knowledge of the crack tip chemistry is crucial for understanding fracture in these materials. Such knowledge can be obtained only from in situ studies because the processes that occur in the highly confined environment of the crack tip are very different from those that take place at free surfaces, or that can be traced post mortem. We report the occurrence of hydrous liquid condensate between the two fracture surfaces in the vicinity of the tip of tensile cracks in silica. Observations are performed in real-time by means of atomic force microscopy (AFM) at continuously controlled crack velocities in the regime of stress corrosion. Condensate formation and changes in extent and shape are demonstrated for a wide range of macroscopic humidity at different crack speeds. Its liquid character is confirmed by the study of AFM phase-contrast data. It is believed that this evidence of a nanoscale liquid hydrous phase at the crack tip will enable novel insights in the chemistry of failure of oxide materials.
ISSN: 0002-7820

hal-00424984
https://hal.archives-ouvertes.fr/hal-00424984
ARXIV : cond-mat/0509209
DOI : 10.1111/j.1551-2916.2005.00765.x

Éditeur(s) : HAL CCSD Wiley
Résumé : International audience
OBJECTIVE: The WEGENT trial and other short-term studies suggested that azathioprine or methotrexate could effectively maintain granulomatosis with polyangiitis (GPA) or microscopic polyangiitis (MPA) remission. Whether differences in relapse or adverse event rates would appear after discontinuation of those 2 maintenance regimens and longer follow-up remains unknown. METHODS: Long-term outcomes for the patients enrolled in the WEGENT trial were analyzed according to their randomization group. Parameters at trial entry were evaluated as potential prognostic factors for death, relapse or damage in multivariate models. RESULTS: Data were returned for 88.8% of the 126 original participants. Median [95% confidence interval] followup was 11.9 [11.3-12.5] years. For the azathioprine and methotrexate arms, respectively, the 10-year overall survival rates were 75.1% [64.8-86.9] and 79.9% [70.3-90.8] (P = 0.56), and relapse-free survival rates 26.3% [17.3-40.1] and 33.5% [23.5-47.7] (P = 0.29). No between-arm differences were observed for relapse, adverse events, damage, survival rates without severe side effects and survival rates without relapse and severe side effects. Considering only the 97 GPA patients, no between-arm survival differences were observed. Relapse-free survival was shorter for GPA than MPA patients but the multivariate analysis retained anti-PR3-ANCA-positivity, and not GPA, as being independently associated with relapse. CONCLUSION: This long-term analysis confirms that azathioprine and methotrexate are comparable options for maintaining GPA or MPA remission. Despite good overall survival, relapses, adverse events and damage remain matters of concern and further studies are needed to reduce them.
ISSN: 2326-5205

hal-01217989
https://hal-univ-rennes1.archives-ouvertes.fr/hal-01217989
https://hal-univ-rennes1.archives-ouvertes.fr/hal-01217989/document
https://hal-univ-rennes1.archives-ouvertes.fr/hal-01217989/file/Long-term%20outcomes%20of%20the%20WEGENT%20trial_accepted.pdf
DOI : 10.1002/art.39450

Éditeur(s) : HAL CCSD Springer Verlag
Résumé : International audience
The New Jersey continental shelf extends 150 km off the shoreline. During IODP Expedition 313, siliciclastic deposits of late Eocene to late Pleistocene age were drilled down to 631, 669 and 755 m below seafloor at sites 27A, 28A and 29A respectively in very shallow waters (33.5 to 36 m depth). Pore water salinities display multilayered brackish-salty-brine units 10 to 170 m thick, where low-salinity water is preferentially stored in fine-grained sediments. The sharp boundaries of these buried aquifers are often marked by cemented layers a few centimetres thick. The mineralogy and scanning electron microscope observations of these layers show two phases of cementation by authigenic minerals: (1) the early carbonate cement is frequently associated with pyrite, and (2) the late silicate cement infills the residual porosity. The isotopic compositions of the carbonate cements vary widely: −2.4 < δ¹⁸O ‰ VPDB < +2.8; −15.1 < δ¹³C ‰ VPDB < +15.6. The δ¹⁸O values indicate that the carbonate cements precipitated with pore waters comprising variable mixtures of seawater and ¹⁸O-depleted fresh water originating from submarine groundwater discharge. The δ¹³C values of the carbonate cements are related to organic matter diagenesis, providing ¹³C-depleted dissolved inorganic carbon during bacterial sulphate reduction and anaerobic oxidation of methane, and ¹³C-rich dissolved inorganic carbon during methanogenesis. The diagenetic cementation processes included chemical weathering of reactive silicate minerals by the CO²-rich pore waters issued from organic matter diagenesis that released bicarbonate, cations and dissolved silica, which were further precipitated as carbonate and silicate cements. The estimated range of temperature (18±4 °C) during carbonate precipitation is consistent with carbonate cementation at moderate burial depths; however, silicate cementation occurred later during diagenesis at deeper burial depths.
ISSN: 0276-0460

hal-01559985
https://hal.archives-ouvertes.fr/hal-01559985
DOI : 10.1007/s00367-017-0506-6

Éditeur(s) : HAL CCSD Elsevier
Résumé : The slow advance of a crack in soda-silicate glasses was studied at nanometer scale by in-situ and real-time atomic force microscopy (AFM) in a well-controlled atmosphere. An enhanced diffusion of sodium ions in the stress-gradient field at the sub-micrometric vicinity of the crack tip was revealed through several effects: growth of nodules in AFM height images, changes in the AFM tip–sample energy dissipation. The nodules patterns revealed a dewetting phenomenon evidenced by ‘breath figures'. Complementary chemical micro-analyses were done. These experimental results were explained by a two-step process: (i) a fast migration (typical time: few milliseconds) of sodium ions towards the fracture surfaces as proposed by Langford et al. [J. Mat. Res. 6 (1991) 1358], (ii) a slow backwards diffusion of the cations as evidenced in these AFM experiments (typical time: few minutes). Measurements of the diffusion coefficient of that relaxing process were done at room temperature. Our results strengthen the theoretical concept of a near-surface structural relaxation due to the stress-gradient at the vicinity of the crack tip. The inhomogeneous migration of sodium ions might be a direct experimental evidence of the presence of sodium-rich channels in the silicate structure [A. Meyer et al., Phys. Rev. Let. 93 (2004) 027801].
ISSN: 0022-3093

hal-00407634
https://hal.archives-ouvertes.fr/hal-00407634
DOI : 10.1016/j.jnoncrysol.2006.09.034

Éditeur(s) : HAL CCSD Oxford University Press (OUP)
Résumé : International audience
Energy dissipation is observed on seismic data when a wave propagates through a porous medium, involving different frequency regimes depending on the nature of rock and fluid types. We focus here on the role of partial fluid saturation in unconsolidated porous media, looking in particular at P-wave phase velocity and attenuation. The study consists in running an experiment in a sand-filled tank partially saturated with water. Seismic propagation in the tank is generated in the kHz range by hitting a steel ball on a granite plate. Seismic data are recorded by buried accelerometers and injecting or extracting water controls the partial saturation. Several imbibition/drainage cycles were performed between the water and gas residual saturations. A Continuous Wavelet Transform applied on seismic records allowed us to extract the direct P-wave at each receiver.We observe an hysteresis in phase velocities and inverse quality factors between imbibition and drainage. Phase velocities and inverse quality factors are then jointly inverted to get a final poro-visco-elastic model of the partially saturated sand that satisfactorily reproduces the data. The model formulation consists in generalizing the Biot theory to effective properties of the fluid and medium (permeability and bulk modulus) in order to properly explain the phase velocity variation as a function of the saturation. The strong level of attenuation measured experimentally is further explained by an anelastic effect due to grain to grain sliding, adding to Biot's losses. This study shows that fluid distribution at microscopic scale has strong influence on the attenuation of direct P-waves at macroscopic scale and confirms that seismic prospection may be a powerful tool for the characterization of transport phenomena in porous media.
ISSN: 0956-540X

hal-00779920
https://hal.archives-ouvertes.fr/hal-00779920
https://hal.archives-ouvertes.fr/hal-00779920/document
https://hal.archives-ouvertes.fr/hal-00779920/file/Barriere_et_al_HAL.pdf
DOI : 10.1111/j.1365-246X.2012.05691.x

Éditeur(s) : HAL CCSD
Résumé : International audience
Numerous diffusion models can be found in the literature. Although these models havebeen widely used to address several problems related to diffusion phenomena at the individuallevel, they do not faithfully represent the microscopic mechanisms that take place in the realtransmission processes. Today, the apparition of new communication media allows model togo further by providing data on many real cases of diffusion. In particular, these newapproaches have shown that several dimensions involved in diffusion phenomena are notpresent in current models. In our works, we focus on two original dimensions to address thediffusion problem: the personality and the neutrality of the spreader. Our aim is to understandthe influence of these two dimensions regarding the diffusion behaviours. Our approach hasbeen used to study diffusion of messages related to several events occurred in January 2015.The results obtained provide a new and interesting lighting on the heterogeneity of thediffusion behaviors in function of personality and neutrality levels. This work provides amethodology which may be useful in marketing strategies, for instance the analysis of thefeatures of individuals who talk about their touristic trips in Caribbean. Such an approach mayalso be useful to understand diffusion and exchange behaviors in the Caribbean basin. Froman economic point of view, such an approach could be used for understanding theexpectations, targeting individuals and proposing tailored offers.
20th Biennial General Meeting of The Caribbean Academy of Sciences
Deshaies, France

hal-01514262
https://hal.archives-ouvertes.fr/hal-01514262

Éditeur(s) : HAL CCSD
Résumé : International audience
Millimeter waves (MMW) will be increasingly used for future wireless telecommunications. Previous studies on skin keratinocytes showed that MMW could impact the mRNA expression of Transient Receptor Potential cation channel subfamily Vanilloid, member 2 (TRPV2). Here, we investigated the effect of MMW exposure on this marker, as well as on other membrane receptors such as Transient Receptor Potential cation channel subfamily Vanilloid, member 1 (TRPV1) and purinergic receptor P2X, ligand-gated ion channel, 3 (P2 × 3). We exposed the Neuroscreen-1 cell line (a PC12 subclone), in order to evaluate if acute MMW exposures could impact expression of these membrane receptors at the protein level. Proteotoxic stress-related chaperone protein Heat Shock Protein 70 (HSP70) expression level was also assessed. We used an original high-content screening approach, based on fluorescence microscopy, to allow cell-by-cell analysis and to detect any cell sub-population responding to exposure. Immunocytochemistry was done after 24 h MMW exposure of cells at 60.4 GHz, with an incident power density of 10 mW/cm2. Our results showed no impact of MMW exposure on protein expressions of HSP70, TRPV1, TRPV2, and P2 × 3. Moreover, no specific cell sub-populations were found to express one of the studied markers at a different level, compared to the rest of the cell populations. However, a slight insignificant increase in HSP70 expression and an increase in protein expression variability within cell population were observed in exposed cells, but controls showed that this was related to thermal effect. Bioelectromagnetics. 37:444–454, 2016. © 2016 Wiley Periodicals, Inc. © 2016 Wiley Periodicals, Inc.
Bioelectromagnetics

hal-01371963
https://hal-univ-rennes1.archives-ouvertes.fr/hal-01371963
DOI : 10.1002/bem.21995
PUBMED : 27483046

Éditeur(s) : HAL CCSD Elsevier
Résumé : In order to address the question of the processes involved during shear zone nucleation, we present a petro-structural analysis of millimetre-scale shear zones within the Roffna rhyolite (Suretta nappe, Eastern central Alps). Field and microscopic evidences show that ductile deformation is localized along discrete fractures that represent the initial stage of shear zone nucleation. During incipient brittle deformation, a syn-kinematic metamorphic assemblage of white mica + biotite + epidote + quartz precipitated at ca. 8.5 ± 1 kbar and 480 ± 50 °C that represent the metamorphic peak conditions of the nappe stacking in the continental accretionary wedge during Tertiary Alpine subduction. The brittle to ductile transition is characterized by the formation of two types of small quartz grains. The Qtz-IIa type is produced by sub-grain rotation. The Qtz-IIb type has a distinct CPO such that the orientation of c-axis is perpendicular to the shear fracture and basal and rhombhoedric slip systems are activated. These Qtz-IIb grains can either be formed by recrystallization of Qtz-IIa or by precipitation from a fluid phase. The shear zone widening stage is characterized by a switch to diffusion creep and grain boundary sliding deformation mechanisms. During the progressive evolution from brittle nucleation to ductile widening of the shear zone, fluid–rock interactions play a critical role, through chemical mass-transfer, metasomatic reactions and switch in deformation mechanisms.
ISSN: 0191-8141

hal-01355954
https://hal.archives-ouvertes.fr/hal-01355954
DOI : 10.1016/j.jsg.2016.02.015

Éditeur(s) : HAL CCSD Paris : Muséum national d'histoire naturelle
Résumé : International audience
En prenant comme exemple le gobie à crête Lophogobius cyprinoides (Pallas, 1770), les éléments (canaux et pores et canaux sensoriels muqueux, et papilles sensorielles) composant le système latéral (SL) ont été observés selon plusieurs méthodes de préparation. Des échantillons ont été préparés soit pour observation en microscopie électronique à balayage (MEB), soit pour observation en microscopie photonique après immersion dans du liquide de fixation "Bouin Hollande" ou dans du formol à 4% en eau de mer avant coloration au KMnO4 (technique traditionnelle). Les résultats obtenus avec le MEB et le Bouin Hollande sont meilleurs qu'avec la méthode traditionnelle et présentent une plus grande reproductibilité. Bien que moins rapide et plus onéreuse, la méthode d'évaluation au MEB offre une finesse d'observation qui n'est pas obtenue au Bouin Hollande en raison de la limite du grandissement en microscopie optique. Si l'ultrastructure des papilles sensorielles ne peut être observable qu'au MEB, le SL des poissons Gobiidae peut être facilement étudié de manière approfondie avec les deux méthodologies proposées dans ce travail. Elles sont à préconiser car plus fiables que la technique traditionnelle consistant en une immersion dans une solution de permanganate de potassium et d'acide sulfurique. Celle-ci détériore partiellement les papilles sensorielles du SL rendant son analyse plus délicate.
ISSN: 0399-0974

hal-00749624
https://hal.univ-antilles.fr/hal-00749624

Éditeur(s) : HAL CCSD
Résumé : International audience
A key element of the biomechanical design of trees is their ability to generate largemechanical stresses in wood at the stem periphery. This function is necessary for the tree tocontrol the orientation of its axes, and therefore to grow in height, maintain its branches at anoptimal angle or achieve adaptive reorientations (Fournier et al., 2013). This “maturationstress” appears in wood fibres during their cellular maturation when their secondary cell wallis thickening. In hardwoods, the stress asymmetry is generated by the formation of specificfibres with a very high tensile growth stress on the upper side of the inclined axis. Theresulting tension wood has microstructural features highly different from the normal wood.Tension wood in almost all temperate species has a specific gelatinous cell wall layer, calledG-layer, that is not lignified and highly mesoporous (Chang et al., 2015). It has been shownrecently, at the macroscopic scale, that cellulose tension appears during maturation and issynchronous with the development of this specific layer (Clair et al., 2011). Tropical species,like simarouba, are able to generate lignified tension wood fibres. The mechanisms of stressgeneration in tension wood fibres are not yet well known. The aim of our study is tounderstand these mechanisms and to know the spatial and temporal kinetics of the differentcell wall layers stiffening during maturation, in parallel with tensile stress generation.Contact-Resonance Atomic Force Microscopy, CR-AFM, was used here to address thisquestion on embedded samples (Arnould and Arinero, 2015) using dual resonance frequencytracking (DRFT, Rodriguez et al., 2007). Change in the contact modulus between, and within,each layer of the cell wall has been measured on several radial lines of developing fibres atdifferent steps. In order to understand the obtained results, and to estimate the sensitivity ofthe AFM indentation-like technique to the different cell wall components stiffness, an elasticanisotropic indentation model was used (Vlassak et al., 2003; Jäger et al., 2011). Finally,these mechanical measurements have been compared to topochemical data obtained on thesame fibres.
8th Plant Biomechanics International Conference
Nagoya, Japan

hal-01436113
https://hal.archives-ouvertes.fr/hal-01436113

Éditeur(s) : HAL CCSD Wiley-Blackwell
Résumé : International audience
In marine invertebrates that acquire their symbionts from the environment, these are generally only taken up during early developmental stages. In the symbiosis between lucinid clams and their intracellular sulfur-oxidizing bacteria, it has been shown that the juveniles acquire their symbionts from an environmental stock of free-living symbiont forms, but it is not known if adult clams are still competent to take up symbiotic bacteria from the environment. In this study, we investigated symbiont acquisition in adult specimens of the lucinid clam Codakia orbiculata, using transmission electron microscopy, fluorescence in situ hybridization, immunohistochemistry and PCR. We show here that adults that had no detectable symbionts after starvation in aquaria for 6 months, rapidly reacquired symbionts within days after being returned to their natural environments in the field. Control specimens that were starved and then exposed to seawater aquaria with sulfide did not reacquire symbionts. This indicates that the reacquisition of symbionts in the starved clams returned to the field was not caused by high division rates of a small pool of remaining symbionts that we were not able to detect with the methods used here. Immunohistochemistry with an antibody against actin, a protein involved in the phagocytosis of intracellular bacteria, showed that actin was expressed at the apical ends of the gill cells that took up symbionts, providing further evidence that the symbionts were acquired from the environment. Interestingly, actin expression was also observed in symbiontcontaining cells of untreated lucinids freshly collected from the environment, indicating that symbiont acquisition from the environment occurs continuously in these clams throughout their lifetime.
ISSN: 1462-2912

hal-00743019
https://hal.univ-antilles.fr/hal-00743019
DOI : 10.1111/j.1462-2920.2012.02748.x

Éditeur(s) : HAL CCSD ACS American Chemical Society - Publications
Résumé : International audience
ABSTRACT: In this paper, the structural, optical and thermal properties of n-type (100),p-type (100) and (111) mesoporous silicon (MePSi) are reported. The mesoporous silicon wasprepared by an electrochemical process from bulk silicon wafer. Depending on the etchingdepth, analyses show that the porosity of p-type (111) increased by 32 to 40% compared to p(100) which, in turn, increased by 22 to 48% compared to n-type (100). The structuremorphology and the abundance of Si-Ox and Si-Hy also depended heavily on the type andcrystal orientation of MePSi. The thermal properties of the MePSi layers such as thermalconductivity (κ), volumetric heat capacity (ρCp) and thermal contact resistance (Rth) weredetermined using the pulsed photothermal method. The thermal conductivity of bulk silicondropped sharply after etching, decreasing by more than twenty-fold in the case of n-type (100)and by over forty-five fold for p-type (100) and (111). According to the percolation modeldepending on both porosity and phonon confinement, the drop in thermal conductivity wasmainly due to the nanostructure formation after etching. Thermal investigations showed thatthe volumetric heat capacity (ρCp) followed the barycentric model which depends mainly onthe porosity. The thermal contact resistances of MePSi layers were estimated to be in therange of 1x10-8 to 1x10-7 K⋅m2⋅W-1.
The Journal of Physical Chemistry C

hal-01495490
https://hal.archives-ouvertes.fr/hal-01495490
DOI : 10.1021/acs.jpcc.6b13101

Éditeur(s) : HAL CCSD Public Library of Science
Résumé : 51 pages; 15figures; 3 tables, accepted for publication in PloS ONE
International audience
We present a study about AFM imaging of living, moving or self-immobilized, bacteria in their genuine physiological liquid medium. No external immobilization protocol, neither chemical nor mechanical, was needed. For the first time, the native gliding movements of Gram-negative Nostoc cyanobacteria upon the surface, at speeds up to 900microns/h, were studied by AFM. This was possible thanks to an improved combination of a gentle sample preparation process and an AFM procedure based on fast and complete force-distance curves made at every pixel, drastically reducing lateral forces. No limitation in spatial resolution or imaging rate was detected. Gram-positive and non-motile R. wratislaviensis bacteria were studied as well. From the approach curves, Young modulus and turgor pressure were measured for both strains at different gliding speeds and are ranging from 20±3 to 105±5MPa and 40±5 to 310±30kPa depending on the bacterium and the gliding speed. For Nostoc, spatially limited zones with higher values of stiffness were observed. The related spatial period is much higher than the mean length of Nostoc nodules. This was explained by an inhomogeneous mechanical activation of nodules in the Nostoc. We also observed the presence of a soft extra cellular matrix (ECM) around the Nostoc. Both strains left a track of polymeric slime with variable thicknesses. For R. wratislaviensis, it is equal to few hundreds of nanometers, likely to promote its adhesion to the sample. While gliding, the Nostoc secretes a slime layer the thickness of which is in the nanometer range and increases with the gliding speed.
ISSN: 1932-6203

hal-00769765
https://hal.archives-ouvertes.fr/hal-00769765
https://hal.archives-ouvertes.fr/hal-00769765v3/document
https://hal.archives-ouvertes.fr/hal-00769765/file/REsubmission_In-situ_determination_Dhahri_Plos_One_R2_Hal.pdf

Éditeur(s) : HAL CCSD IEEE
Résumé : International audience
This paper proposes a novel automated approach for the categorization of cells in fluorescence microscopy images. Our supervised classification method aims at recognizing patterns of unlabeled cells based on an annotated dataset. First, the cell images need to be indexed by encoding them in a feature space. For this purpose, we propose tailored bio-inspired features relying on the distribution of contrast information. Then, a supervised learning algorithm is proposed for classifying the cells. We carried out experiments on cellular images related to the diagnosis of autoimmune diseases, testing our classification method on the HEp-2 Cells dataset of Foggia et al (CBMS 2010). Results show classification precision larger than 96% on average, thus confirming promising application of our approach to the challenging application of cellular image classification for computer-aided diagnosis.
ICPR 2012
Tsukuba, Japan

hal-00958856
https://hal.archives-ouvertes.fr/hal-00958856
https://hal.archives-ouvertes.fr/hal-00958856/document
https://hal.archives-ouvertes.fr/hal-00958856/file/icpr_2012.pdf