Éditeur(s) : Université des Antilles AREBio Groupe de recherche BIOSPHERES : BIOlogie, Sciences Physiques & Humaines pour les énergies Renouvelables, l
Extrait de : 1er colloque international BIOSPHERES, du 18 au 20 juin 2019. Université des Antilles
Description : The Chemical Purification, analysis and Screening core laboratories (CPAS) are located at the University of South Florida campus in Tampa. This core offers instrumentation and expertise in fractionation, purification, and various chemical analyses to support synthetic and natural products drug discovery research groups. Our main equipment are dedicated to chromatographic separation and purification (MPLC, HPLC) and mass-spectrometry for analytic detection, quantification and identification (LC-MS SQ, QqQ and QToF). The CPAS services also include Circular Dichroism spectrometry, general spectroscopic characterization (UV, FTIR), lyophilization (bench top and high capacity freeze-dryers), synthesis (microwave reactor), ADME screening (permeability and drug solubility testing), and high-throughput screening development capability (automated liquid handler and multimode plate reader). An example of three significant studies will be presented at the conference to illustrate the CPAS core facility services: 1) Identification of new bioactive compounds from mangrove endophytic fungi as potential alternatives to malaria drug resistance, 2) Myricanol isolated from Bayberry (Myrica Cerifera) and its synthetic derivatives targeting the microtubuleassociated protein tau accumulation in the Alzheimer's disease and 3) New ent-labdane diterpenes with solid tumor cell lines antiproliferative properties from the endemic plant Eupatorium obtusissmum from the island of Hispaniola. The core provides research lab training workshops in advanced technology and assistance so users can operate any instruments available in the laboratories to successfully accomplish their analyses and research projects.

Droits : CC-BY-NC-ND - Attribution - Pas d'utilisation commerciale - Pas de modification
Permalien : http://www.manioc.org/fichiers/V19063
V19063

Éditeur(s) : HAL CCSD Elsevier
Résumé : International audience
The active collision zone around Taiwan orogen receives a variety of sedimentary inputs, including terrigenous flux from Taiwan and Chinese rivers, oceanic currents and atmospheric dust. In order to determine the present-day respective contributions of these different sources, we analysed the Pb and Sr isotopic compositions of late Quaternary sediments accumulated offshore east Taiwan. Samples from the Taiwan Coastal Range (andesites, sandstones, conglomerate and Peinan River terraces and suspended loads) have also been analysed to constrain the mean Taiwan isotopic signature. Mixing lines between the different sources of material indicate that the core located in the Okinawa Trough represents a mixture of about 60% of Taiwan, 30% of Chinese Loess and 10% of Yangtze River contributions. The southernmost core, located close to Luzon Are, is influenced by Taiwan (60%) and volcanic material (40%). The Sr and Pb isotopic compositions of samples cored in the Manila Trench and in the Huatung Basin are strongly influenced by the Taiwan signature, while the Ryukyu Trench core samples point to significant but variable contributions of Chinese Loess and Yangtze River. This work also reports Zn isotopic variations in the silicate fraction of these marine core samples. The overall variation in Zn isotopic compositions (expressed as 666 Zn) is greater than 0.3 per mil (parts per thousand) for an analytical precision of +/- 0.05 parts per thousand. The Zn isotopic signal for most of the marine core samples is similar to the literature data measured on sedimentary material, except for the Manila Trench core samples and the older sample from the Huatung Basin: they present values heavier by 0.2-0.3 parts per thousand, close to those obtained on all Taiwan rocks and Peinan River particulates. These results show: 1- the particular characteristics of Taiwan Zn isotopic compositions on a large scale; and: 2- the possible use of Zn isotopes as a tracer of material sources.
ISSN: 0009-2541

hal-00412214
https://hal.archives-ouvertes.fr/hal-00412214
DOI : 10.1016/j.chemgeo.2007.10.024

Éditeur(s) : HAL CCSD PeerJ
Résumé : International audience
The adaptative bleaching hypothesis (ABH) states that, depending on the symbiotic flexibility of coral hosts (i.e., the ability of corals to ''switch'' or ''shuffle'' their algal symbionts), coral bleaching can lead to a change in the composition of their associated Symbiodinium community and, thus, contribute to the coral's overall survival. In order to determine the flexibility of corals, molecular tools are required to provide accurate species delineations and to detect low levels of coral-associated Symbiodinium. Here, we used highly sensitive quantitative (real-time) PCR (qPCR) technology to analyse five common coral species from Moorea (French Polynesia), previously screened using only traditional molecular methods, to assess the presence of low-abundance (background) Symbiodinium spp. Similar to other studies, each coral species exhibited a strong specificity to a particular clade, irrespective of the environment. In addition, however, each of the five species harboured at least one additional Symbiodinium clade, among clades A–D, at background levels. Unexpectedly, and for the first time in French Polynesia, clade B was detected as a coral symbiont. These results increase the number of known coral-Symbiodinium associations from corals found in French Polynesia, and likely indicate an underestimation of the ability of the corals in this region to associate with and/or ''shuffle'' different Symbiodinium clades. Altogether our data suggest that corals from French Polynesia may favor a trade-off between optimizing symbioses with a specific Symbiodinium clade(s), maintaining associations with particular background clades that may play a role in the ability of corals to respond to environmental change. How to cite this article Rouzé et al. (2017), An updated assessment of Symbiodinium spp. that associate with common scleractinian corals from Moorea (French Polynesia) reveals high diversity among background symbionts and a novel finding of clade B.
ISSN: 2167-8359

Droits : info:eu-repo/semantics/OpenAccess
hal-01430294
https://hal-sde.archives-ouvertes.fr/hal-01430294
https://hal-sde.archives-ouvertes.fr/hal-01430294/document
https://hal-sde.archives-ouvertes.fr/hal-01430294/file/Rouze-2017-PeerJ-Updated.pdf
DOI : 10.7717/peerj.2856

Éditeur(s) : Yale University
Résumé : Sea-surface temperature images of the coastal upwelling regions off Northwest Africa show that the core of upwelling is sometimes located far from the coast. This has been documented in three regions that share a common feature, namely a wide and shallow continental shelf. This upwelling feature plays a key role in the ecology of the Canary Current System. It creates an innerfront which provides retention for biological material, e.g. fish eggs and larvae, in the highly productive nearshore environment. An analytical model has been developed based on a two dimensional extension of Ekman's solution. The linear and steady response of a homogeneous ocean forced by an upwelling-favorable wind provides a mechanism for the upwelling separation from the coast. The merging of the surface and bottom Ekman layers induces a very weak cross-shore circulation and a "kinematic barrier" for the Ekman transport divergence. In the case of an alongshore wind, the barrier is located near the isobath h ≈ 0.4D, where D is the thickness of Ekman layers. This yields an upwelling cell which is essentially concentrated in the region 0.5D < h < 1.25D, with upwelling occurring preferentially near the isobath h ≈ 0.6D. It turns out that the cross-shore width of upwelling scales with D/S, the ratio of Ekman depth to bottom topographic slope. The application of this solution to real bathymetric profiles rationalizes, not only the offshore upwelling observations in Northwest Africa, but also the influence of topography on the cross-shelf structure of a wind-driven coastal upwelling. The model also quantifies the effect of the cross-shore wind component showing how it drives the nearshore pressure gradient adjustment and how it affects the upwelling. A linear numerical experiment reproduces the theoretical steady solution, thereby allowing investigation of the transient regime. Relaxation of the hypothesis in the numerical model validates the linear assumption of the theory and then allows investigation of the sensitivity to friction parameterizations and the influence of stratification. The latter leads to an "oscillation" of the upwelling cell with seaward migration driven by outcropping and homogeneization of the water column, and, coastal incursion driven by a "boundary layers splitting" process caused by shoreward advection of the isopycnal dome and stratification of the inner shelf.
Journal of Marine Research (Yale University), 2008-09 , Vol. 66 , N. 5 , P. 589-616

Droits : 2008 Yale University
http://archimer.ifremer.fr/doc/2008/publication-6334.pdf
DOI:10.1357/002224008787536790
http://archimer.ifremer.fr/doc/00000/6334/

Éditeur(s) : HAL CCSD PeerJ
Résumé : International audience
Understanding the role of predators in food webs can be challenging in highly diverse predator/prey systems composed of small cryptic species. DNA based dietary analysis can supplement predator removal experiments and provide high resolution for prey identification. Here we use a metabarcoding approach to provide initial insights into the diet and functional role of coral-dwelling predatory fish feeding on small invertebrates. Fish were collected in Moorea (French Polynesia) where the BIOCODE project has generated DNA barcodes for numerous coral associated invertebrate species. Pyrosequencing data revealed a total of 292 Operational Taxonomic Units (OTU) in the gut contents of the arc-eye hawkfish (Paracirrhites arcatus), the flame hawkfish (Neocirrhites armatus) and the coral croucher (Caracanthus maculatus). One hundred forty-nine (51%) of them had species-level matches in reference libraries (>98% similarity) while 76 additional OTUs (26%) could be identified to higher taxonomic levels. Decapods that have a mutualistic relationship with Pocillopora and are typically dominant among coral branches, represent a minor contribution of the predators' diets. Instead, predators mainly consumed transient species including pelagic taxa such as copepods, chaetognaths and siphonophores suggesting non random feeding behavior. We also identified prey species known to have direct negative interactions with stony corals, such as Hapalocarcinus sp, a gall crab considered a coral parasite, as well as species of vermetid snails known for their deleterious effects on coral growth. Pocillopora DNA accounted for 20.8% and 20.1% of total number of sequences in the guts of the flame hawkfish and coral croucher but it was not detected in the guts of the arc-eye hawkfish. Comparison of diets among the three fishes demonstrates remarkable partitioning with nearly 80% of prey items consumed by only one predator. Overall, the taxonomic resolution provided by the metabarcoding approach highlights a highly complex interaction web and demonstrates that levels of trophic partitioning among coral reef fishes have likely been underestimated. Therefore, we strongly encourage further empirical approaches to dietary studies prior to making assumptions of trophic equivalency in food web reconstruction. How to cite this article Leray et al. (2015), Metabarcoding dietary analysis of coral dwelling predatory fish demonstrates the minor contribution of coral mutualists to their highly partitioned, generalist diet. PeerJ 3:e1047
ISSN: 2167-8359

Droits : http://creativecommons.org/licenses/by/
hal-01174184
https://hal-univ-perp.archives-ouvertes.fr/hal-01174184
https://hal-univ-perp.archives-ouvertes.fr/hal-01174184/document
https://hal-univ-perp.archives-ouvertes.fr/hal-01174184/file/peerj-1047.pdf
DOI : 10.7717/peerj.1047

Éditeur(s) : HAL CCSD Elsevier
Résumé : International audience
The aim of this study was to establish a new QuEChERS (quick, easy, cheap, effective, rugged and safe) method coupled to gas chromatography-mass spectrometry (GC-MS) detection for the evaluation of the pesticide biodistribution in specific maternal and fetal tissues. This method was validated for the quantification of pesticides such as chlorotriazines (atrazine, simazine and propazine), their chlorinated metabolites (DIA, DEA and DACT). This new QuEChERS method was developed to facilitate extraction from small tissues such as fetal tissues (mean value: 200mg). The limits of detection, quantification, recovery, precision and accuracy were evaluated for different tissues (liver and brain) and blood. LOD and LOQ ranged between 0.34 and 3.27 ng/g and 1.04 to 9.91 ng/g, respectively. Recovery exceeded 80% for all pesticides, except DACT, with an associated RSD<15%. Precision and accuracy satisfied the criteria usually applied in the validation of bioanalytical methods. The results obtained indicate that this technique is suitable for use in studies of the biodistribution of pesticides in fetal tissues and can be used to evaluate the risk of exposure to pesticides during gestation.
ISSN: 0731-7085

hal-01122837
https://hal-univ-rennes1.archives-ouvertes.fr/hal-01122837
PUBMED : 25497890
DOI : 10.1016/j.jpba.2014.10.035

Éditeur(s) : HAL CCSD Integrated Ocean Drilling Program
Résumé : Integrated Ocean Drilling Program (IODP) Expedition 325, designed to investigate the fossil reefs on the shelf edge of the Great Barrier Reef, was the fourth expedition to utilize a mission-specific platform and was conducted by the European Consortium for Ocean Research Drilling (ECORD) Science Operator (ESO). The objectives of Expedition 325 were to establish the course of sea level change, define sea-surface temperature variations, and analyze the impact of these environmental changes on reef growth and geometry for the region over the period of 20-10 ka. To meet these objectives, a succession of fossil reef structures preserved on the shelf edge seaward of the modern barrier reef were cored from a dynamically positioned vessel in February-April 2010. A total of 34 boreholes across 17 sites were cored in depths ranging from 42.27 to 167.14 meters below sea level (lowest astronomical tide taken from corrected EM300 multibeam bathymetry data). Borehole logging operations in four boreholes provided continuous geophysical information about the drilled strata. The cores were described during the Onshore Science Party (OSP) at the IODP Bremen Core Repository (Germany) in July 2010, where minimum and some standard measurements were made. Preliminary postcruise dating of core catcher samples and initial observations of the cores made during the OSP confirm that coral reef material ranging in age from >30,000 to 9,000 calendar years before present (years before 1950 AD) was recovered during Expedition 325. Further postcruise research on samples taken during the OSP is expected to fulfill the objectives of the expedition.
ISSN: 1930-1014

hal-00855800
https://hal.archives-ouvertes.fr/hal-00855800

Éditeur(s) : HAL CCSD Geological Society
Résumé : International audience
Palaeoclimate and sea-level studies commonly rely on interpretations based on depth. The location and quantity of core recovered during a drilling expedition has direct implications for subsequent applications of core measurements. A key objective of Integrated Ocean Drilling Program (IODP) Expedition 3 10 is to establish the course of postglacial sea-level rise for Tahiti in the South Pacific, for which assigning the correct depth to recovered core is fundamental. By convention, core is placed at the top of the core barrel run from which it was collected. Unless core recovery is 100%, this results in depth errors that are inversely proportional to the recovery. The difficulties of locating core are compounded for the Tahiti boreholes, where primary cavities in the carbonate reef successions are frequently present. Only through the integration of core with continuous geophysical measurements of the borehole can accurate depth positioning be achieved. High-resolution optical and acoustic images of the borehole wall allow effective visual correlation of the core and logging datasets. The final integrated depths form the underlying framework for all subsequent scientific analyses of recovered core employing evaluations that rely on depth. Furthermore, the integration process allows true core recoveries to be accurately estimated.
ISSN: 0016-7649

hal-00411915
https://hal.archives-ouvertes.fr/hal-00411915
DOI : 10.1144/0016-76492007-041

Éditeur(s) : HAL CCSD MDPI
Résumé : International audience
Marine ecosystems cover about 70% of the planet surface and are still an underexploited source of useful metabolites. Among microbes, filamentous fungi are captivating organisms used for the production of many chemical classes of secondary metabolites bound to be used in various fields of industrial application. The present study was focused on the collection, isolation, screening and genotyping of pigmented filamentous fungi isolated from tropical marine environments around La Réunion Island, Indian Ocean. About 150 micromycetes were revived and isolated from 14 marine samples (sediments, living corals, coral rubble, sea water and hard substrates) collected in four different locations. Forty-two colored fungal isolates belonging to 16 families, 25 genera and 31 species were further studied depending on their ability to produce pigments and thus subjected to molecular identification. From gene sequence analysis, the most frequently identified colored fungi belong to the widespread Penicillium, Talaromyces and Aspergillus genera in the family Trichocomaceae (11 species), then followed by the family Hypocreaceae (three species). This study demonstrates that marine biotopes in La Réunion Island, Indian Ocean, from coral reefs to underwater slopes of this volcanic island, shelter numerous species of micromycetes, from common or uncommon genera. This unstudied biodiversity comes along with the ability for some fungal marine inhabitants, to produce a range of pigments and hues.
ISSN: 2309-608X

hal-01657071
http://hal.univ-reunion.fr/hal-01657071
http://hal.univ-reunion.fr/hal-01657071/document
http://hal.univ-reunion.fr/hal-01657071/file/14-%20Fouillaud%20et%20al.%2C%20J.%20of%20Fungi%202017.pdf
DOI : 10.3390/jof3030036

Éditeur(s) : Elsevier
Résumé : We investigated the influence of bioturbation by macrofauna on the vertical distribution of living (stained) benthic foraminifera in marine intertidal sediments. We investigated the links between macrofaunal bioturbation and foraminiferal distribution, by sampling from stations situated on a gradient of perturbation by oyster-farming, which has a major effect on benthic faunal assemblages. Sediment cores were collected on the French Atlantic coast, from three intertidal stations: an oyster farm, an area without oysters but affected by oyster biodeposits, and a control station. Axial tomodensitometry (CT-scan) was used for three-dimensional visualization and two-dimensional analysis of the cores. Biogenic structure volumes were quantified and compared between cores. We collected the macrofauna, living foraminifera, shells and gravel from the cores after scanning, to validate image analysis. We did not investigate differences in the biogenic structure volume between cores. However, biogenic structure volume is not necessarily proportional to the extent of bioturbation in a core, given that many biodiffusive activities cannot be detected on CT-scans. Biodiffusors and larger gallery-diffusors were abundant in macrofaunal assemblage at the control station. By contrast, macrofaunal assemblages consisted principally of downward-conveyors at the two stations affected by oyster farming. At the control station, the vertical distribution of biogenic structures mainly built by the biodiffusor Scorbicularia plana and the large gallery-diffusor Hediste diversicolor was significantly correlated with the vertical profiles of living foraminifera in the sediment, whereas vertical distributions of foraminifera and downward-conveyors were not correlated at the station affected by oyster farming. This relationship was probably responsible for the collection of foraminifera in deep sediment layers (> 6 cm below the sediment surface) at the control station. As previously suggested for other species, oxygen diffusion may occur via the burrows built by S. plana and H. diversicolor, potentially increasing oxygen penetration and providing a favorable microhabitat for foraminifera in terms of oxygen levels. By contrast, the absence of living foraminifera below 6 cm at the stations affected by oyster farming was probably associated with a lack of biodiffusor and large gallery-diffusor bioturbation. Our findings suggest that the effect of macrofaunal bioturbation on the vertical distribution of foraminiferal assemblages in sediments depends on the effects of the macrofauna on bioirrigation and sediment oxidation, as deduced by Eh values, rather than on the biogenic structure volume produced by macrofauna. The loss of bioturbator functional diversity due to oyster farming may thus indirectly affect infaunal communities by suppressing favorable microhabitats produced by bioturbation.
Journal of Experimental Marine Biology and Ecology (0022-0981) (Elsevier), 2009-03 , Vol. 371 , N. 1 , P. 20-33

Droits : 2009 Elsevier B.V. All rights reserved.
http://archimer.ifremer.fr/doc/2009/publication-6146.pdf
DOI:10.1016/j.jembe.2008.12.012
http://archimer.ifremer.fr/doc/00000/6146/

Éditeur(s) : HAL CCSD Public Library of Science
Résumé : International audience
Understanding how communities respond to natural disturbances is fundamental to assess the mechanisms of ecosystem resistance and resilience. However, ecosystem responses to natural disturbances are rarely monitored both through space and time, while the factors promoting ecosystem stability act at various temporal and spatial scales. Hence, assessing both the spatial and temporal variations in species composition is important to comprehensively explore the effects of natural disturbances. Here, we suggest a framework to better scrutinize the mechanisms underlying community responses to disturbances through both time and space. Our analytical approach is based on beta diversity decomposition into two components, replacement and biomass difference. We illustrate this approach using a 9-year monitoring of coral reef fish communities off Moorea Island (French Polynesia), which encompassed two severe natural disturbances: a crown-of-thorns starfish outbreak and a hurricane. These disturbances triggered a fast logistic decline in coral cover, which suffered a 90% decrease on all reefs. However, we found that the coral reef fish composition remained largely stable through time and space whereas compensatory changes in bio-mass among species were responsible for most of the temporal fluctuations, as outlined by the overall high contribution of the replacement component to total beta diversity. This suggests that, despite the severity of the two disturbances, fish communities exhibited high resistance and the ability to reorganize their compositions to maintain the same level of total community biomass as before the disturbances. We further investigated the spatial congruence of this pattern and showed that temporal dynamics involved different species across sites; yet, herbivores controlling the proliferation of algae that compete with coral communities were consistently favored. These results suggest that compensatory changes in bio-mass among species and spatial heterogeneity in species responses can provide further
ISSN: 1932-6203

hal-01212344
https://hal-univ-perp.archives-ouvertes.fr/hal-01212344
https://hal-univ-perp.archives-ouvertes.fr/hal-01212344/document
https://hal-univ-perp.archives-ouvertes.fr/hal-01212344/file/journal.pone.0138696.pdf
DOI : 10.1371/journal.pone.0138696

Éditeur(s) : HAL CCSD
Résumé : International audience
Mylonite bodies are exhumed extensions at depth of shallow structural level brittle faults. They are characterised by intense strain localisation involving large volumes of middle to lower continental crust. The analysis of shear zone geometry and microstructures is a useful tool to decipher deformation kinematics, though finite strain estimation is still relatively qualitative. Timing of the deformation has also been the focus of numerous studies using the complete spectrum of geochronometers. However, only rare examples provide geochronological data that can be unambiguously interpreted and cooling, recrystallisation and neocrystallisation are difficult to tell from each other. Thus, time-scales over which major mylonite zones develop and remain active under ductile conditions as well as their strain rate often remain poorly documented. We investigated two sections across the South-Armorican Shear Zone (SASZ), a crustal-scale pluri-km thick dextral shear zone active during a single tectonic event of late-Variscan age and associated with syntectonic granite emplacement.. Finite strain profiles on these cross-sections, quantified from average Quartz grain-size in 34 samples, show a strong deformation partitioning towards the center of the SASZ, with a decrease, for each section, from 150µm in slightly deformed zones to less than 7-5 µm in the core of the ultramylonite. Furthermore, toward the core of the SASZ, development of a penetrative shear band network progressively eliminates lenses of less deformed rocks and results in homogenisation of the Quartz grain sizes. In parallel, we performed in-situ UV-laser Ar/Ar dating on the fabric-forming white micas. White micas population can be decomposed into inherited muscovites carried by the folation and newly-formed, syn-tectonic substituted phengites located along the shear bands yielding 2 age groups for each sample. Along the profiles, the difference between these two ages evolves from 10-15 Ma in weakly deformed domains to zero toward the SASZ core. In addition, mylonite-forming phengites yielded the same age irrespectively of their position on the section or of the rock strain.. On the regional point of view, this study brings deformation-ages for the late-orogenic evolution of the South Armorican domain. Strain accumulated along the SASZ in a time-span extending to 300-298 Ma, i.e. contemporaneously with the activity of nearby major extensional shear zones. These results support a new model of crustal-scale shear zone activity, where the whole body of the shear zone is active throughout its history (defined as the largest age difference through the section), i.e. the actively deforming zone does not get progressively narrower. The strain distribution nevertheless evolves with time, with an increasingly large fraction partitioned in the ultramylonitic core.
EGU General Assembly
Vienne, Austria

insu-00839320
https://hal-insu.archives-ouvertes.fr/insu-00839320
BIBCODE : 2010EGUGA..12.9881A

Éditeur(s) : Soc Francaise D Ichtyologie
Résumé : The ichtyofauna of Geyser and Zelee coral banks (north of Mozambique canal) was investigated in 1996, this work being the first quantitative study on this area. Quantitative data were collected on 11 stations (mean depth 18 m) by visual observations along 50 m transect, widened to 2 m for small sedentary species, and to 5 m for commercial species with a large action range. The main descriptors of the fish communities (species richness, abundance per species) and the trophic structure (omnivorous, herbivorous, carnivorous, browsers of sessile invertebrates) were described and analysed.A total of 294 fish species, belonging to 43 families were recorded. The species richness varied from 45 to 83 species / station, and the abundance, from 292 to 1322 individuals / 100 m(2), the lower values being recorded on the inner slope and the higher ones on the outer slope. In terms of number of individuals, diurnal planktivores were highly represented at all sites (mean 63.7%). Pomacentridae and Caesionidae were the most abundant families, damselfishes being mainly represented by Chromis ternatensis (mean 73 ind., max, 148 ind. 100 m(2)), C. dimidiata (mean 33 ind., max. 151 ind. / 100 m(2)) and Dascyllus carneus (mean 38 ind., max. 92 ind. 100 m(2)), and fusiliers by Pterocaesio chrysozona (mean 19 ind., max. 128 ind. / 100 m(2)), P. tile (mean 12 ind., max. 38 ind. / 100 m(2)) and Caesio teres (mean 6 ind., max. 32 ind. / 100 m(2)). The high values recorded showed that small and isolated coral banks can support relatively rich fish faunas and substantial populations of individuals. This result may be explained by different factors such as the oceanographic circulation favourable to a localised enrichment of nutrients from surface water, the low human impact, a good coral cover and a high degree of self-recruitment associated with regional recruitment. Nevertheless, the main commercial species such as Lutjanus bohar and Variola louti, observed at each station, were represented at a low density (mean 0.8 ind., max. 6 ind. / 100 m(2) and mean 0.2 ind., max. 0.8 ind. / 100 m(2) respectively). This result may be due to the exploitation of these coral banks between 1990 and 1992, which was interrupted following the fast decreasing output.Such an experience shows us the fragility of these small coral banks and the necessity to establish a rational management of the resource.
Cybium (0399-0974) (Soc Francaise D Ichtyologie), 2002 , Vol. 26 , N. 1 , P. 11-26

Droits : info:eu-repo/semantics/openAccess
http://archimer.ifremer.fr/doc/00000/10132/7687.pdf
http://archimer.ifremer.fr/doc/00000/10132/

Éditeur(s) : Gauthier-Villars
Résumé : Mud fields located In the inner part of shelves in front of large estuaries are preferential environments for the investigation of the mechanisms and evolution in time of the supply of such sedimentary bodies. The study of different constituents of the particulate organic matter trapped in the sediments of the West Gironde mud patch contributes to the knowledge of the input origins. This study was carried out with different techniques, including pyrolysis-gas chromatography-mass spectrometry and high performance liquid chromatography, on samples from a core collected in the centre of the mud field. At the bottom of the core, the increase of sugars, amino-sugars and some aromatic hydrocarbons may indicate a more marine character of organic components. The same appears to be the case at the top of the core, where, despite a finer sedimentation, biochemical characteristics could show a more pronounced marine influence than in the middle part. In the middle part of the core, on the other hand, the increase in phenols could result from an increase in lignin-derived. terrestrial inputs. The increase in pyrolysis-derived cyclopentenone may indicate the same terrestrial influence, because this component has its origin in aliphatic polycarboxylic acids resulting from sail leaching. The continental character of the sedimentation is also reflected in the increase of the silt-clay fractions representative of suspended matter originating in the Gironde estuary, associated with a slight carbonate decrease. The taxonomic significance of some phenols is noteworthy: this is especially the case of the cinnamyls which, in the middle part of the core, emphasize the input of herbaceous debris and of the hydroxybenzyls which, at the bottom, provide evidence of the algal origin of part of the organic material. Thus, by means of pyrolysis results and HPLC phenol analysis, it is possible to establish the more or less continental signature of organic components trapped in the ''Ouest-Gironde'' mud patch. This study of organic matter, which confirms earlier sedimentological and micropaleontological data, constitutes a new approach, adapted to the identification of the origin of carbon sources.
Oceanolica Acta (0399-1784) (Gauthier-Villars), 1997 , Vol. 20 , N. 5 , P. 687-695

Droits : info:eu-repo/semantics/openAccess
http://archimer.ifremer.fr/doc/00093/20426/18093.pdf
http://archimer.ifremer.fr/doc/00093/20426/

Éditeur(s) : Université des Antilles Études caribéennes
Résumé : This paper aims to explore the complex relationships between international tourism and the production and exchange of 'nature' in tropical countries. It is based on data gathered through an ethnographic case study approach on the changing aesthetic, economic and symbolic values attached to the coral reef in the tropical island of La Réunion, Indian Ocean. The analysis of this data shows that, from the point where the collective imaginaries in the Western world associate a 'magic' value with corals, an international institutionalisation process takes place in order to protect and preserve coral reefs and make them touristically accessible. This raises important issues related to the challenges the new value of tropical nature represents for the communication and exchange systems of communities in tropical countries. The paper first explores the meanings of the spiritual and religious value Western societies attribute to coral reefs. It then discusses the institutionalisation of tropical nature by international organisations and the problem of parallel land and natural space ownership systems in La Réunion. After that, it deconstructs the mystification policy of the coral reef operated by local institutions in La Réunion and examines the meaning of protecting the coral reef as a socially embedded 'communicative action' in La Réunion.
L’objectif de cette étude est d’explorer les relations complexes entre d'une part le tourisme international et d'autre part la production et l'échange de 'nature' dans des pays tropicaux. L'étude est basée sur des données recueillies à travers une approche ethnographique des valeurs esthétiques, économiques et symboliques attachées au récif corallien de l'île de La Réunion, dans l'océan Indien. L'analyse de ces données montre qu'à partir du moment où les imaginaires collectifs de l'Occident associent les coraux à une valeur 'magique', un processus d'institutionnalisation internationale s’engage dans le but de protéger, préserver et rendre accessibles au tourisme les récifs coralliens. Ceci implique des problématiques importantes liées à la mise à l'épreuve des systèmes de communication et d'échange des sociétés dans les pays tropicaux. L'étude explore d'abord la signification des valeurs spirituelles et religieuses qu'attribuent les sociétés occidentales aux récifs coralliens. Ensuite, elle discute l'institutionnalisation de la nature tropicale par des organisations internationales ainsi que le problème de l'existence de différents systèmes de gestion foncière à La Réunion. Enfin, l'étude déconstruit la politique de mythification du récif corallien mise en œuvre par des institutions locales à La Réunion et examinera la signification de 'protéger l'environnement' en tant qu'action communicante définie par les systèmes de communication et d'échange réunionnais.
Réunion

Droits : info:eu-repo/semantics/openAccess
urn:doi:10.4000/etudescaribeennes.1112
http://journals.openedition.org/etudescaribeennes/1112

Éditeur(s) : HAL CCSD
Résumé : Satellite and in situ chlorophyll concentration data collected as part of VALidation HYperspectral of a BIOgeochemical model (ValHyBio), a PNTS-sponsored project dedicated to satellite ocean-colour imaging of the Southwest Pacific, are analyzed to describe chlorophyll variability in the Coral Sea, a vast oligotrophic region characterized by a deep chlorophyll maximum and blue waters of high transparency. Average chlorophyll concentrations estimated from MODIS-Aqua are very low (<0.2 mg m-3) except in the vicinity of main islands and coral reefs, where enrichment may occur due to upwelling or internal waves that bring nutrients to the euphotic zone from the deep part of the water column. During the summer season, blooms of cyanobacteria (Trichodesmium) develop in the surface waters and may form large slicks. The nitrogen fixed by these slicks is a potential source of new nitrogen later assimilated by picoplankton and the marine food chain. Coastal areas exhibit higher chlorophyll concentrations because of nutrient input from the land, as observed in 2008 around New Caledonia. In lagoon areas, the sea colour is influenced by turbidity and bathymetry, and the MODIS OC3 algorithm is inadequate, with a systematic overestimation of chlorophyll concentration in the New Caledonia lagoon. Improved bio-optical algorithms are needed for those turbid and shallow waters, to allow not only a better description of chlorophyll variability, but also evaluation of chlorophyll simulations by recently developed biogeochemical models.
of the 12th International Coral Reef Symposium, 18E
CAIRNS, Australia

hal-00746470
https://hal.archives-ouvertes.fr/hal-00746470
https://hal.archives-ouvertes.fr/hal-00746470/document

Éditeur(s) : Elsevier France-editions Scientifiques Medicales Elsevier
Résumé : The essential characteristics of the vegetation dynamics of tropical Africa remain only partially known. This study assesses the succession of vegetation-types over Central Africa during the last two glacial/interglacial cycles. Analysis of core KZai 02, which contains pollen from the Zaire River watershed (latitudes 9°N-13°S), allows the investigation of long-term patterns of plant ecosystem development and their climatic causes. Core KZai 02 (18.20 m long) was recovered from 6°24.20′S/9°54.10′E in the uppermost axial edifice of the Zaire deep sea fan. The chronology of this sedimentary archive was established using nannofossils and correlations of pollen and total organic carbon signals with the nearby core GeoB1008. The pollen record indicates that: (i) glacials (MIS 6, 4, 2) are marked by the development of afromontane (Podocarpus) forest at high altitudes when central basin lowlands were occupied by Cyperaceae marshes and savannah; (ii) during interglacials (MIS 1, 5) lowland forests were developed, marked by the successive expansion of pioneer, warm-temperate, rain forests, and mangrove indicating sea-level rise; (iii) glacial-interglacial transitions (MIS 6/5, 2/1) display similar vegetation dynamics. The strong evidence of afromontane forest and the opening of the vegetation during glacials suggest a reduced latitudinal distribution of rainfall by the strengthening of the trade wind system. West African monsoon systems were enhanced during interglacials, allowing the progressive development of lowland forests. The development of rain and pioneer forests during glacial Heinrich stadials suggests an enhancement of water availability in tropical Africa associated with these high latitude events. However, no augmentation of wind activity, described by previous studies, is evidenced by our pollen record. Similar vegetation successions during glacial/interglacial transitions suggest the diachronous and stepped intervention of CO2 (emphasizing the influence of temperature on plant ecosystems) and water availability.
Geobios (0016-6995) (Elsevier France-editions Scientifiques Medicales Elsevier), 2014-07 , Vol. 47 , N. 4 , P. 183-198

Droits : 2014 Elsevier Masson SAS. All rights reserved.
http://archimer.ifremer.fr/doc/00199/31031/29436.pdf
DOI:10.1016/j.geobios.2014.06.002
http://archimer.ifremer.fr/doc/00199/31031/

Éditeur(s) : HAL CCSD Elsevier
Résumé : International audience
The influence of redox alteration on the growth and proteomic pattern of Listeria monocytogenes was investigated. A redox shock was induced in cultures by addition of 3mM ferricyanide (FeCN) and 6mM dithiothreitol (DTT) to increase or to decrease respectively the redox potential naturally occurring at the beginning of growth. In both conditions, the reducing and oxidizing redox shock had a strong influence, decreasing the maximum growth rate by half compared to a control culture. The proteomic analysis of L. monocytogenes performed by two-dimensional difference gel electrophoresis (2D-DIGE) exhibited twenty-three proteins differentially expressed (P<0.05), among these, many were oxidoreductases, and proteins involved in cellular metabolism (glycolysis, protein synthesis), detoxification (kat) or adhesion (Lmo1634).
ISSN: 1874-3919

hal-00877138
https://hal-univ-rennes1.archives-ouvertes.fr/hal-00877138
DOI : 10.1016/j.jprot.2012.11.010
PUBMED : 23195917

Éditeur(s) : HAL CCSD Wiley
Résumé : International audience
High sea surface temperature accompanied by high levels of solar irradiance is responsible for the disruption of the symbiosis between cnidarians and their symbiotic dinoflagellates from the genus Symbiodinium. This phenomenon, known as coral bleaching, is one of the major threats affecting coral reefs around the world. Because an important molecular trigger to bleaching appears related to the production of reactive oxygen species (ROS), it is critical to understand the function of the antioxidant network of Symbiodinium species. In this study we investigated the response of two Symbiodinium species, from contrasting environments, to a chemically induced oxidative stress. ROS produced during this oxidative burst reduced photosynthesis by 30 to 50% and significantly decreased the activity of superoxide dismutase. Lipid peroxidation levels and carotenoid concentrations, especially diatoxanthin, confirm that these molecules act as antioxidants and contribute to the stabilization of membrane lipids. The comparative analysis between the two Symbiodinium species allowed us to highlight that Symbiodinium sp. clade A temperate was more tolerant to oxidative stress than the tropical S. kawagutii clade F. These differences are very likely a consequence of adaptation to their natural environment, with the temperate species experiencing conditions of temperature and irradiance much more variable and extreme.
ISSN: 0140-7791

hal-01546161
https://hal.archives-ouvertes.fr/hal-01546161
DOI : 10.1111/pce.12825

Éditeur(s) : John Wiley & Sons Ltd
Résumé : 1. The development of macroalgae to the detriment of corals is now one of the major threats to coral reefs. Herbivorous fishes are partly responsible for algal regulation on coral reefs and their overexploitation favours the shift from scleractinian coral-dominated systems towards macroalgae-dominated systems. 2. Marine protected areas (MPAs) that have been established worldwide may benefit coral reefs through the maintenance of high densities of herbivorous fishes which regulate algal growth. 3. The paper assesses whether small MPAs in the Caribbean are able to enhance herbivorous fish stock and by controlling macroalgae help to maintain reef ecosystems. A visual census using band-transects was undertaken around Guadeloupe island where marine reserves have been in place since 1979. The effects of MPAs on both benthic communities and herbivorous fishes are examined. 4. Inside MPAs, herbivorous fish biomass was almost twice as high as outside MPAs and macroalgal cover was significantly lower. Fish size class distributions revealed that large individuals occurred mainly inside MPAs and that few male individuals were found outside MPAs. Copyright (C) 2010 John Wiley & Sons, Ltd.
Aquatic Conservation-marine And Freshwater Ecosystems (1052-7613) (John Wiley & Sons Ltd), 2010-07 , Vol. 20 , N. 5 , P. 516-524

Droits : 2010 John Wiley & Sons, Ltd.
http://archimer.ifremer.fr/doc/00011/12214/9551.pdf
DOI:10.1002/aqc.1118
http://archimer.ifremer.fr/doc/00011/12214/