Éditeur(s) : Inter-Research
Résumé : On the western coast of the Atlantic, according to the literature, 2 distinct groups of Semibalanus balanoides occur with a distinct interface near the Miramichi Estuary, New Brunswick, in the Gulf of St. Lawrence. On each side of this interface, the groups are characterized by clinal variations for MPI (mannose-6-phosphate isomerase) and GPI (glucose-6-phosphate isomerase). The present study was carried out to determine whether selection occurs at this interface, to establish how early in the sessile life period it occurs and to examine the selecting forces involved. Reciprocal transplant experiments of newly settled individuals to both sides of the interface were carried out. No significant differences specifically linked to source or destination were observed in growth or fecundity for the 2 groups at the sites studied for either control or transplanted individuals. However, differences in survival were observed; individuals transplanted south of the estuary showed lower survival than individuals transplanted north. An allozyme analysis of barnacle survivors for MPI and GPI, 2 alleles whose frequencies are known to vary abruptly in this region, indicated a change of allele frequency in transplanted individuals. The transplants’ allele frequencies came to resemble those of adults from target sites, while no change occurred in transplanted individuals at control sites. Taken together with previous results, our study suggests that selection occurs very early in the newly settled individuals (spat).
Marine Ecology Progress Series (0171-8630) (Inter-Research), 2002-03 , Vol. 229 , P. 173-184

Droits : Inter-Research 2002
http://archimer.ifremer.fr/doc/00048/15970/13410.pdf
DOI:10.3354/meps229173
http://archimer.ifremer.fr/doc/00048/15970/

Éditeur(s) : Inter-research
Résumé : Environmental conditions experienced by aquatic organisms are archived in biogenic carbonates such as fish otoliths, bivalve shells and coral skeletons. These calcified structures present an accretionary growth and variations in optical properties - color or opacity - that are used to reconstruct time. Full and reliable exploitation of the information extracted from these structures is, however, often limited as the metabolic processes that control their growth and their optical properties are poorly understood. Here, we propose a new modeling framework that couples both the growth of a biogenic carbonate and its optical properties with the metabolism of the organism. The model relies on well-tested properties of Dynamic Energy Budget (DEB) theory. It is applied to otoliths of the Bay of Biscay anchovy (Engraulis encrasicolus) for which a DEB model has been previously developed. The model reproduces well-known otolith patterns and thus provides us with mechanisms for the metabolic control of otolith size and opacity at the scale of an individual lifespan. Two original contributions using this framework are demonstrated. First, the model can be used to reconstruct the temporal variations in the food assimilated by an individual fish. Reconstructing food conditions of past and present aquatic species in their natural environment is key ecological information to better understand population dynamics. Second, we show that non-seasonal checks can be discriminated from seasonal checks, which is a well-recognized problem when interpreting fish otoliths. We discuss further developments of the model and the experimental settings required to test this new promising framework.
Marine Ecology-progress Series (0171-8630) (Inter-research), 2012 , Vol. 447 , P. 151-164

Droits : 2012 Inter-Research
http://archimer.ifremer.fr/doc/00060/17082/14593.pdf
DOI:10.3354/meps09492
http://archimer.ifremer.fr/doc/00060/17082/

Éditeur(s) : HAL CCSD Nordic Ecological Society
Résumé : Niche differentiation and ecological filtering are primary ecological processes that shape community assembly, but their relative importance remains poorly understood. Analyses of the distributions of functional traits can provide insight into the community structure generated by these processes. We predicted the trait distributions expected under the ecological processes of niche differentiation and environmental filtering, then tested these predictions with a dataset of 4672 trees located in nine 1-ha plots of tropical rain forest in French Guiana. Five traits related to leaf function (foliar N concentration, chlorophyll content, toughness, tissue density and specific leaf area), and three traits related to stem function (trunk sapwood density, branch sapwood density, and trunk bark thickness), as well as laminar surface area, were measured on every individual tree. There was far more evidence for environmental filtering than for niche differentiation in these forests. Furthermore, we contrasted results from species-mean and individual-level trait values. Analyses that took within-species trait variation into account were far more sensitive indicators of niche differentiation and ecological filtering. Species-mean analyses, by contrast, may underestimate the effects of ecological processes on community assembly. Environmental filtering appeared somewhat more intense on leaf traits than on stem traits, whereas niche differentiation affected neither strongly. By accounting for within-species trait variation, we were able to more properly consider the ecological interactions among individual trees and between individual trees and their environment. In so doing, our results suggest that the ecological processes of niche differentiation and environmental filtering may be more pervasive than previously believed.
ISSN: 0030-1299

hal-01032397
https://hal.archives-ouvertes.fr/hal-01032397
DOI : 10.1111/j.1600-0706.2010.19110.x

Éditeur(s) : ISTPM
Résumé : Shell is the basis of the distinction between the different species of molluscs. But the elements defining the shape and colour of the shell can vary according to the environmental conditions. So much so that, in numerous cases, a whole range of intermediates can be found between two species. Among molluscs, oysters are considered as a particularly confused group. Their shells are indeed extremely variable. All sorts of intermediates can be found. They usually attach themselves using their bottom valve, and, depending on the shape of the substrate, salinity conditions, temperature, outside environment, they present a wide range of individual variations. I had the opportunity to examine the larval shells of some of our coastal species. I noticed that each species had a specific prodissoconch, with a constant shape and no fundamental individual variations, an understandable fact given their planktonic life. The hinge and the position of the ligament in the hinge enable the definition of their genus. During the study of the collections of oysters from some of the world's most renowned museum (1), I tried to isolate the prodissoconches of the different species. I found them attached to the adult shells, making sure that all the prodissoconches attached to the top of the adult shells showed the same morphological characteristics. (unverified OCR)
LAMARCK, en 1806, dans l'ouverture de son Cours sur les «Animaux sans Vertèbres», s'exprimait ainsi : «En attendant, souvenons-nous que rien de tout cela n'est dans la nature, qu'elle ne connaît ni Classes, ni Ordres, ni Genres, ni Espèces, malgré le fondement que paraissent leur donner les portions de la série naturelle que nous offrent nos collections et que parmi les corps organisés, il n'y a réellement que des individus et des races diverses qui se nuancent dans tous les degrés de l'organisation. » Ainsi, pour LAMARCK, les cadres de la Classification zoologique seraient artificiels, ce seraient des créations de l'esprit, ayant seulement pour but de faciliter l'étude du monde vivant. Effectivement lorsqu'on consulte, comme je l'ai fait, les «Collections Lamarck» des Musées de Paris et de Genève. on s'aperçoit que, pour LAMARCK, le moindre caractère morphologique qui différencie une coquille de Mollusque d'une autre est une entité. Il multipliait le nombre des espèces et créait des variétés. Pour CUVIER (2) : «Le travail des nomenclatures, besogne facile, qui ne demande aucune intelligence a fait négliger la science véritable.» Et dit-il, ailleurs (:1) : «Après tout je n'ai pas jeté cette esquisse de division pour servir aux commençans à trouver les noms des espèces; qu'ils emploient pour cela tel système artificiel qu'ils trouveront le plus facile; cela est juste... » Les systèmes en question ne seraient donc que jeux de l'esprit, pour CUVIER également. Notre pensée a beaucoup évolué depuis cette époque. Nous pouvons dire maintenant que la Vie est la manière d'être des protéines et qu'il existe autant de protéines que d'espèces. Dès 1912, Jacques LOEB disait (4) : «Qu'en toute certitude, chaque animal a des substances germinatives spécifiques et que les substances germinatives diffèrent chimiquement d'un animal à l'autre, Les qualités chimiques d'un oeuf de poulet font qu'il ne peut donner naissance qu'à un poulet.» La notion d'espèce chimique vivante nous entraîne, par ailleurs, vers une nouvelle conception des autres groupes de la classification. L'individu porteur de caractères particuliers par lesquels il peut seulement être défini, est aussi porteur d'un noyau protoplasmique spécifique et enfin d'autres, propres successivement à la famille, à l'ordre, à la classe et à l'embranchement dont il fait partie. Cela est très important, car toutes ces catégories de la classification zoologique ne nous apparaissent plus alors comme de simples entités, subjectives, formelles, mais comme ayant effectivement une réalité précise dans le monde extérieur. La recherche et la définition correcte des espèces apparaissent alors comme un travail de première importance. Lorsqu'on poursuit des recherches embryologiques, physiologiques, biologiques ou autres sur un organisme, il est indispensable de savoir d'une manière précise en présence de quelle espèce on se trouve. Par ailleurs, comment parler de l'évolution des espèces si leur existence objective n'est pas admise et si elles ne sont pas correctement définies? Toute l'histoire de la Zoologie est marquée par l'effort constant de préciser les caractères de l'espèce et d'en faire une réalité objective. Chez les Mollusques, la coquille a servi de base essentielle pour la distinction des espèces. Mais les éléments de la forme et de la couleur de la coquille varient avec les conditions du milieu de telle sorte qu'on trouve dans de nombreux cas, tous les intermédiaires entre deux espèces. Parmi les Mollusques, les Huîtres constituaient un groupe particulièrement confus. En effet, leurs coquilles sont extrêmement variables. On rencontre tous les intermédiaires. Elles se fixent le plus souvent sur un support par la valve inférieure et là selon la forme du support, selon les conditions de la salinité, de la température, du milieu extérieur, elles présentent une immense gamme de variations individuelles. J'ai été amené à examiner les coquilles larvaires de quelques espèces de nos côtes. J'ai constaté que chaque espèce avait une prodissoconque particulière, d'une forme constante et sans variations individuelles fondamentales ce qui se conçoit aisément, étant donné leur vie planctonique. Leur charnière et la position du ligament par rapport à cette dernière, permettent de définir les genres, En étudiant les collections d'Huîtres des grands musées du monde (1), j'ai cherché à isoler les prodissoconques des diverses espèces. Je les ai trouvées sur les coquilles adultes où elles s'étaient fixées. m'assurant chaque fois que les prodissoconques conservées au sommet des coquilles adultes présentaient les mêmes caractéristiques morphologiques. J'ai microphotographié la plupart des coquilles larvaires étudiées. Ce sont les épreuves qui sont reproduites ici. J'en ai fait faire des dessins au trait et pour quelques-unes dont les microphotographies manquent, on trouvera les dessins au trait réalisé à la chambre claire. Chaque espèce est bien définie par sa coquille larvaire. La meilleure démonstration en est fournie par les deux espèces. si voisines par leurs coquilles adultes, Ostrea edulis et Ostrea chilensis; leurs prodissoconques sont spectaculairement différentes. Je remercie Monsieur le Directeur de l'Institut scientifique et technique des Pêches maritimes qui a bien voulu accepter de publier le résultat de ces recherches dans la belle Revue des Travaux de son Institut. (OCR non contrôlé)
Revue des Travaux de l'Institut des Pêches Maritimes (0035-2276) (ISTPM), 1967-06 , Vol. 31 , N. 2 , P. 127-199

Droits : Ifremer
http://archimer.ifremer.fr/doc/1967/publication-3761.pdf
http://archimer.ifremer.fr/doc/00000/3761/

Éditeur(s) : HAL CCSD European Geosciences Union
Résumé : Xylem density is a physical property of wood that varies between individuals, species and environments. It reflects the physiological strategies of trees that lead to growth, survival and reproduction. Measurements of branch xylem density, ρx, were made for 1653 trees representing 598 species, sampled from 87 sites across the Amazon basin. Measured values ranged from 218 kg m−3 for a Cordia sagotii (Boraginaceae) from Mountagne de Tortue, French Guiana to 1130 kg m−3 for an Aiouea sp. (Lauraceae) from Caxiuana, Central Pará, Brazil. Analysis of variance showed significant differences in average ρx across regions and sampled plots as well as significant differences between families, genera and species. A partitioning of the total variance in the dataset showed that species identity (family, genera and species) accounted for 33% with environment (geographic location and plot) accounting for an additional 26%; the remaining "residual" variance accounted for 41% of the total variance. Variations in plot means, were, however, not only accountable by differences in species composition because xylem density of the most widely distributed species in our dataset varied systematically from plot to plot. Thus, as well as having a genetic component, branch xylem density is a plastic trait that, for any given species, varies according to where the tree is growing in a predictable manner. Within the analysed taxa, exceptions to this general rule seem to be pioneer species belonging for example to the Urticaceae whose branch xylem density is more constrained than most species sampled in this study. These patterns of variation of branch xylem density across Amazonia suggest a large functional diversity amongst Amazonian trees which is not well understood.
ISSN: 1726-4170

hal-01032137
https://hal.archives-ouvertes.fr/hal-01032137

Éditeur(s) : Blackwell science
Résumé : The trophic level mean and variance, and the degree of omnivory for five Celtic Sea fish predators were estimated using a database of stomach content records characterized by a high level of taxonomic resolution. The predators occupied a high position in the food web, i.e. 4.75 for Atlantic cod Gadus morhua, 444 for haddock Melanogrammus aeglefinus, 4.88 for European hake Merluccius merluccius, 5.00 for megrim Lepidorhombus whiffiagonis and 5.27 for whiting Merlangius merlangus. The level of taxonomic resolution of the prey did not greatly affect mean T-L predator values; an effect on variance was evident, low resolution masking intra-population variability in T-L. Generalized additive models (GAM) were used to explain the variability of predator T-L caused by environmental variables (international Council for the Exploration of the Sea, ICES, division and season) and predator characteristics (total length, L-T). Significant year, location season and interaction effects were found for some species and with L-T, at the scale of ICES subdivision. The species-specific variability of T-L could be due to spatio-temporal variations in prey availability and in predator selectivity following ontogenetic changes. Omnivorous fish T-L. was less affected by spatio-temporal variations. In addition, results showed that the omnivory index and T-L variability provide dissimilar information on predator feeding strategy. Combining information on T-L. variability and omnivory allowed between within individual and between-individual components contributing to trophic niche width to be separated and the type of generalization of fish predators to be identified. (c) 2008 The Authors Journal compilation (c) 2008 The Fisheries Society of the British Isles.
Journal of Fish Biology (0022-1112) (Blackwell science), 2008-09 , Vol. 73 , N. 4 , P. 763-781

Droits : 2008 Wiley Blackwell Publishing, Inc. The Fisheries Society of the British Isles
http://archimer.ifremer.fr/doc/2008/publication-4699.pdf
DOI:10.1111/j.1095-8649.2008.01938.x
http://archimer.ifremer.fr/doc/00000/4699/

Éditeur(s) : HAL CCSD Seismological Society of America
Résumé : International audience
Abstract The continuous record of large surface-rupturing earthquakes along the Dead Sea fault brings unprecedented insights for paleoseismic and archaeoseismic research. In most recent studies, paleoseismic trenching documents the late Holocene faulting activity, while tectonic geomorphology addresses the long-term behavior (>10 ka), with a tendency to smooth the effect of individual earthquake rupture events (Mw >7). Here, we combine historical, archaeological, and paleoseismic investigations to build a consolidated catalog of destructive surface-rupturing earthquakes for the last 14 ka along the left-lateral Jordan Valley fault segment. The 120- km-long fault segment limited to the north and the south by major pull-apart basins (the Hula and the Dead Sea, respectively) is mapped in detail and shows five subsegments with narrow stepovers (width < 3 km). We conducted quantitative geomorphology along the fault, measured more than 20 offset drainages, excavated four trenches at two sites, and investigated archaeological sites with seismic damage in the Jordan Valley. Our results in paleoseismic trenching with 28 radiocarbon datings and the archaeoseismology at Tell Saydiyeh, supplemented with a rich historical seismic record, document 12 surface-rupturing events along the fault segment with a mean interval of ∼1160 yr and an average 5 mm=yr slip rate for the last 25 ka. The most complete part of the catalog indicates recurrence intervals that vary from 280 yr to 1500 yr, with a median value of 790 yr, and suggests an episodic behavior for the Jordan Valley fault. Our study allows a better constraint of the seismic cycle and related short-term variations (late Holocene) versus long-term behavior (Holocene and late Pleistocene) of a major continental transform fault.
ISSN: 0037-1106

hal-00747401
https://hal.archives-ouvertes.fr/hal-00747401
DOI : 10.1785/0120100097

Éditeur(s) : HAL CCSD Elsevier
Résumé : International audience
Grey (Halichoerus grypus) and harbour seals (Phoca vitulina) are sympatric seal species, but they display distinct strategies of habitat use and connectivity between haulout sites. The distribution patterns and variations in relative abundance of both species were investigated along the French coast of the English Channel, at the southern limit of their range where seal numbers are increasing. Regular censuses conducted at all main haulout sites in mainland France showed significant seasonal variations at most sites, with more harbour seals counted during summer (breeding and moulting seasons), and more grey seals during summer only in the eastern English Channel. Trends in maximum haulout numbers at haulout sites showed a significant increase over the last five years, ranging from 9.7 to 30.9% per year for harbour seals, and from 5.8% (in the western English Channel) to 49.2% (in the eastern English Channel) per year for grey seals. These rates of increase in grey seal numbers are not linked to local pup production and most probably result from seal movements from the southwest British Isles and the North Sea, respectively. Aerial surveys conducted across the English Channel showed that most seal observations at sea were concentrated in the north-eastern English Channel. Telemetry showed that the 28 harbour seals tracked remained highly coastal, within a radius of 100 km from their haulout sites, and did not move to other known colonies. Grey seals moved much greater distances, reaching up to 1200 km from their capture site. More than half of the 45 grey seals tracked crossed the English Channel, especially during the breeding season, moving to known colonies in the southwest British Isles and the North Sea. Combining individual tracks and long-term surveys of the seal populations allowed a better understanding of the dynamics of these populations and their connectivity at a larger regional scale. The findings provide direct information for the management of grey and harbour seals within the frame of the Marine Strategy Framework Directive, and highlight focus areas where potential interactions between the two species should be monitored.
ISSN: 0967-0645

hal-01509674
https://hal.archives-ouvertes.fr/hal-01509674
DOI : 10.1016/j.dsr2.2017.04.004

Éditeur(s) : Gauthier-Villars
Résumé : Holothuria atra is the most common aspidochirotid holothurian on tropical Indo-Pacific reefs, Asexual reproduction by transverse fission, followed by regeneration, has been studied at Reunion Island (Indian Ocean) and compared with different populations of the Indo-Pacific zone, thus permitting a better identification of the most significant parameters and a better understanding of this reproductive strategy. At Reunion Island, the species is studied at two stations on the same fringing reef: 1) on the back-reef where the fission rate is high (20 % of the population), the individuals small (generally weighting less than 150 g) and the population density high (4/m(2)); and 2) on the reef front, where fission is extremely rare, the mean size of the individuals larger (up to 300 g) and the density low (0.01/m(2)), Different categories of individuals, fissioning (F), after fission, anterior and posterior parts (A and P), and regenerating (Ap and Pa) have been identified from external observations. Dissection has demonstrated the unequal allocation of organs during fission and the variability of the regenerative states, mostly in the anterior part. Concerning fission, the position of the split in an individual is in the anterior half (at 44 % of the total length). The monthly incidence of fission is higher from October to January and in June-July. Fission does not result in an increase in the density of the population. The monthly rates of regenerating individuals originating from anterior (3.7 %) and posterior (5.1 %) parts suggest that survival is higher in the latter instance. The occurrence of asexual reproduction in various populations of this species is discussed in relation to the ecology and the parameters of the populations considered. On the back-reef studied at Reunion Island, the population is subtidal and emersion cannot explain fission. Anthropogenic disturbances are possible triggers of this phenomenon.
Oceanologica Acta (0399-1784) (Gauthier-Villars), 1996 , Vol. 19 , N. 3-4 , P. 209-216

Droits : info:eu-repo/semantics/openAccess
http://archimer.ifremer.fr/doc/00094/20479/18149.pdf
http://archimer.ifremer.fr/doc/00094/20479/

Éditeur(s) : HAL CCSD Wiley/Blackwell
Résumé : International audience
Microsatellites are widely used in population genetics to uncover recent evolutionary events. They are typically genotyped using capillary sequencer, which capacity is usually limited to 9, at most 12 loci for each run, and which analysis is a tedious task that is performed by hand. With the rise of next-generation sequencing (NGS), a much larger number of loci and individuals are available from sequencing: for example, on a single run of a GS Junior, 28 loci from 96 individuals are sequenced with a 30X cover. We have developed an algorithm to automatically and efficiently genotype microsatellites from a collection of reads sorted by individual (e.g. specific PCR amplifications of a locus or a collection of reads that encompass a locus of interest). As the sequencing and the PCR amplification introduce artefactual insertions or deletions, the set of reads from a single microsatellite allele shows several length variants. The algorithm infers, without alignment, the true unknown allele(s) of each individual from the observed distributions of microsatellites length of all individuals. MicNeSs, a python implementation of the algorithm, can be used to genotype any microsatellite locus from any organism and has been tested on 454 pyrosequencing data of several loci from fruit flies (a model species) and red deers (a nonmodel species). Without any parallelization, it automatically genotypes 22 loci from 441 individuals in 11 hours on a standard computer. The comparison of MicNeSs inferences to the standard method shows an excellent agreement, with some differences illustrating the pros and cons of both methods.
ISSN: 1755-098X

hal-01544754
https://hal.archives-ouvertes.fr/hal-01544754
DOI : 10.1111/1755-0998.12467

Éditeur(s) : EDP Sciences
Résumé : The reproductive dynamics of swordfish (Xiphias gladius) was investigated from catches of the Reunion Island-based longline swordfish fishery between 19-25 degrees S and 48-54 degrees E. Thus, 1727 swordfish (size range 75-289 cm, lower jaw-to-fork length, LJFL) were sampled on board commercial fishing vessels during the period of May 1998 to January 2001. Reproductive activity was assessed using macroscopic gonad characteristics, trends of gonadal indexes for both sexes, oocyte size-frequency distributions and microscopic investigation of oocyte development stages. Size at first maturity (L-50) for female and male swordfish was estimated in Indian Ocean for the first time. L50 was estimated at 170 cm and 120 cm (LJFL) for females and males, respectively. Spawning occurred from October to April in the vicinity of Reunion Island where sex ratio of caught populations fluctuated seasonally. The seasonal changes in sex ratio and the incidence of larger individuals at the beginning of the spawning season provide some preliminary indications of synchronized movements of swordfish between spawning grounds and neighboring regions. Our results add significantly to knowledge about the reproductive biology of swordfish in the southwestern Indian Ocean. An overview of the available information on reproductive biology and dynamics of swordfish in Indian Ocean and eastern Pacific indicated that spawning activity is localized in discrete areas, and showed that there is incomplete information on spawning grounds in this extended area.
La dynamique de la reproduction est étudiée chez des espadons (Xiphias gladius) capturés dans l'océan Indien, entre 19–25 °Sud et 48–54 °Est, par les palangriers de l'île de La Réunion. Ainsi, 1727 espadons de tailles comprises entre 75 et 289 cm (de l'extrémité de la mâchoire inférieure à la fourche caudale) ont été échantillonnés à bord de navires professionnels, de mai 1998 à janvier 2001. La reproduction est évaluée en analysant : les caractéristiques macroscopiques des gonades, l'évolution des indices gonadiques des deux sexes, les distributions de fréquence de taille des ovocytes et le développement des ovocytes au niveau microscopique. La taille à première maturité sexuelle (L50) des espadons de l'océan Indien est estimée pour la première fois ; elle atteint 170 cm pour les femelles et 120 cm pour les mâles. La saison de ponte s'étend d'octobre à avril aux abords de l'île tandis que le sexe-ratio fluctue de façon saisonnière. Les variations saisonnières du sexe-ratio et l'apparition de grands individus au début de la saison de ponte suggèrent des déplacements d'espadons entre cette zone de ponte et les régions voisines. Nos résultats apportent des informations fondamentales sur la biologie de la reproduction de l'espadon dans le sud-ouest de l'océan Indien. L'analyse de la bibliographie concernant biologie et la dynamique de la reproduction de l'espadon dans l'océan Indien et dans le Pacifique Est indique que l'activité de ponte est très localisée et que la connaissance des aires de pontes est encore incomplète dans cette région étendue.
Aquatic Living Resources (0990-7440) (EDP Sciences), 2009 , Vol. 22 , N. 1 , P. 45-58

Droits : 2009 EDP Sciences
http://archimer.ifremer.fr/doc/2009/publication-6353.pdf
DOI:10.1051/alr/2009007
http://archimer.ifremer.fr/doc/00000/6353/

Éditeur(s) : HAL CCSD Inter-Research
Résumé : Precise knowledge of lifetime migrations is vital in exploited fish species, since all essential habitats must be protected to maintain sustainable stock levels. The present study used multi-element otolith fingerprints of the gilthead sea bream Sparus aurata (L.) to discriminate its main juvenile and adult habitats in the Languedoc-Roussillon region (Gulf of Lions, northwest Mediterranean) and infer the lifetime migrations of 12 individuals from the area (11 from the present day and 1 from the Roman era). This allowed for the first time the identification of key habitats for the successful completion of the species' life cycle in the Gulf of Lions, and the connectivity between them. Our results revealed that lagoon use by S. aurata is probably ancient (>2500 yr) and confirmed its current commonness. Yet, although most observed migration patterns were in accordance with the migratory behavior previously described for the species, strong inter-individual variations and new patterns in habitat use were detected. At the juvenile stage, a preference for shallow lagoons with low salinities was evidenced. Nevertheless, the first year of life can also be successfully completed in marine conditions. At the adult stage, lagoon use was shown to occur until at least age 4 yr, with periods of lagoon residency of up to 11 mo in a year, often including winter months. Because overwintering in the lagoons was previously thought to be impossible for S. aurata due to low temperatures, this finding has important implications for future stock management, especially since the species breeds in winter.
Marine Ecology Progress Series

hal-00682003
https://hal.archives-ouvertes.fr/hal-00682003
DOI : 10.3354/meps09444

Éditeur(s) : HAL CCSD BioMed Central
Résumé : International audience
Background: Color polymorphism in the nacre of pteriomorphian bivalves is of great interest for the pearl culture industry. The nacreous layer of the Polynesian black-lipped pearl oyster Pinctada margaritifera exhibits a large array of color variation among individuals including reflections of blue, green, yellow and pink in all possible gradients. Although the heritability of nacre color variation patterns has been demonstrated by experimental crossing, little isknown about the genes involved in these patterns. In this study, we identify a set of genes differentially expressed among extreme color phenotypes of P. margaritifera using a suppressive and subtractive hybridization (SSH) method comparing black phenotypes with full and half albino individuals.Results: Out of the 358 and 346 expressed sequence tags (ESTs) obtained by conducting two SSH libraries respectively, the expression patterns of 37 genes were tested with a real-time quantitative PCR (RT-qPCR) approach by pooling five individuals of each phenotype. The expression of 11 genes was subsequently estimated for each individual in order to detect inter-individual variation. Our results suggest that the color of the nacre is partiallyunder the influence of genes involved in the biomineralization of the calcitic layer. A few genes involved in the formation of the aragonite tablets of the nacre layer and in the biosynthesis chain of melanin also showed differential expression patterns. Finally, high variability in gene expression levels were observed within the black phenotypes.Conclusions: Our results revealed that three main genetic processes were involved in color polymorphisms: the biomineralization of the nacreous and calcitic layers and the synthesis of pigments such as melanin, suggesting that color polymorphism takes place at different levels in the shell structure. The high variability of gene expression found within black phenotypes suggests that the present work should serve as a basis for future studies exploringmore thoroughly the expression patterns of candidate genes within black phenotypes with different dominant iridescent colors.
ISSN: 1471-2164

hal-01260141
https://hal.archives-ouvertes.fr/hal-01260141
https://hal.archives-ouvertes.fr/hal-01260141/document
https://hal.archives-ouvertes.fr/hal-01260141/file/Lemer-2015-BMCGenom-Identification.pdf
DOI : 10.1186/s12864-015-1776-x

Éditeur(s) : Ecole Nationale Supérieure Agronomique de Rennes
Résumé : Long-line fishing has been growing on the island of La Réunion since the beginning of the 1990s. The target species is the swordfish (Xiphias gladius), fished mainly in the tropical waters surrounding the island between longitudes 45 degree E and 60 degree E, and latitudes 17 degree S and 27 degree S. From 1998 to 2000, a finalised research programme enabled the collection of precise data concerning the lines' positions and the parameters of the fishing efforts: 3,602 nettings were thus informed by the catches (in numbers of individuals caught) and 12 descriptive variables of the effort. In parallel with this collection effort, satellite maps that described the ocean's landscape of this region of the Indian Ocean exploited by La Réunion's fisheries were made available to sailors and scientists. Extractions from these environmental variables were made at the fishing lines' positions, which allowed us to propose 20 variables describing the environmental conditions of swordfish catches applied to the nettings. In an early part of this study, after a descriptive analysis and a first selection of the variables, we used several GAM (Generalised Additive Models) to model the respective effects of effort and environmental variables on the variability of swordfish catches. We thus highlighted the predominance of factors linked to fishermen (fishing effort) on oceanographic factors in the explanation of this variability. Following these results, a summary on the biology and ecology of the swordfish was proposed in order to draw up hypotheses on the characteristics of this species' population in the Indian Ocean. The aspects linked to the individual behaviour and movements of the population were particularly highlighted. In a latter part of this study and in order to complete the empirical approach by an exploratory constructivist approach, we proposed a modelling tool in order to simulate the possible movements of individuals within the oceanic landscape described by the available satellite maps. After reading in the literature about the various forms of modelling the movements of the large pelagics, we developed an original computer modelling architecture of the multi-agent type that made it possible to manipulate in a dynamic way the satellite maps (and derived products), the individual movements of "animats" whose behaviours were based on this environmental information, and agents that clarify the process of long-line fishing in a geographical way. The computer model that was implemented (MUFINS, for MUlti-Fish INdian ocean Simulator) was next used by calibrating the behaviour of the animats on the real data originally from the analysis of commercial catches proposed in the first part, using robust techniques based on fuzzy logic. The results of the simulations were compared, on the one hand, with the large-scale hypotheses of movement proposed in the first part, and, on the other hand, to swordfish catches both real and corrected for the effect of the fishing effort modelled by the GAM models. Even if the results of these simulations in this study do not allow for proposing convincing interpretations of the underlying processes, the MUFINS tool showed its ability to address questions associated with the swordfish's behaviour in the tropical oceanic landscape and to the effects of scale transfers between the individual level and the collective level. We now envisage its use in a more operational framework, as an interface for dialogue between fishermen and scientists with a goal of sustainable management of the resource.
La peche palangriere s'est developpee a l'ile de La Reunion depuis le debut des annees 1990. L'espece cible est l'espadon (Xiphias gladius), principalement exploite dans les eaux tropicales autour de l'ile entre les longitudes 45 degree E et 60 degree E, et les latitudes 17 degree S et 27 degree S. De 1998 a 2000, un programme de recherche finalisee a permis de collecter des informations precises concernant les positions des lignes et les parametres de l'effort de peche : 3602 filages sont ainsi renseignes par les captures (en nombre d'individus captures) et 12 variables descriptives de l'effort. En parallele de cet effort de collecte, des cartes satellitales ont pu etre mises a la disposition des marins et des scientifiques, decrivant le paysage oceanique de cette region de l'ocean Indien exploitee par la pecherie reunionnaise. Des extractions de ces variables environnementales ont ete effectuees aux positions des lignes de peche, nous permettant de proposer 20 variables descriptives des conditions environnementales de captures d'espadon appliquees aux filages. Dans une premiere partie de ce travail, apres une analyse descriptive et une premiere selection des variables, on a modelise par plusieurs modeles GAM (Generalized Additive Models) les effets respectifs des variables d'effort et des variables environnementales sur la variabilite des captures d'espadon. On a ainsi mis en evidence la predominance des facteurs lies aux pecheurs (effort de peche) sur les facteurs oceanographiques dans le caractere explicatif de cette variabilite. A la suite de ces resultats, une synthese sur la biologie et l'ecologie de l'espadon a ete proposee afin de degager des hypotheses sur les caracteristiques de la population de cette espece dans l'ocean Indien. Les aspects lies au comportement individuel et aux deplacements de la population ont ete particulierement mis en evidence. Dans une deuxieme partie de ce travail et afin de completer l'approche empirique par une approche constructiviste exploratoire, nous avons propose un outil de modelisation afin de simuler les deplacements possibles d'individus au sein du paysage oceanique decrit par les cartes satellitales disponibles. Apres avoir passe en revue dans la litterature les differentes formes de modelisation des deplacements des grands, pelagiques, nous avons developpe une architecture originale de modelisation informatique de type multi-agents permettant de manipuler de maniere dynamique les cartes satellitales (et les produits derives), les mouvements individuels < d'animats >, dont les comportements sont bases sur cette information environnementale, et des agents qui explicitent le processus de capture palangriere de maniere geographique. Le modele informatique ainsi implemente (MUFINS, pour MUlti-Fish INdian ocean Simulator) a ensuite ete utilise en calibrant les comportements des animats sur les donnees reelles issues de l'analyse des captures commerciales proposee en premiere partie, a partir de techniques robustes basee sur la logique floue. Les resultats des simulations ont ete confrontes d'une part aux hypotheses de deplacement a grande echelle proposees en premiere partie, et d'autre part aux captures d'espadon reelles et corrigees de l'effet de l'effort de peche modelise par les modeles GAM. Meme si les resultats de simulations presentes dans ce travail ne permettent pas de proposer d'interpretations convaincantes des processus sous-jacents, l'outil MUFINS a demontre son aptitude a aborder les questions liees au comportement de l'espadon dans le paysage oceanique tropical et aux effets des transferts d'echelle entre le niveau individuel et le niveau collectif. On envisage desormais son utilisation dans un cadre plus operationnel, comme interface de dialogue entre les pecheurs et les scientifiques a des fins de gestion durable de la ressource.

Droits : info:eu-repo/semantics/openAccess
http://archimer.ifremer.fr/doc/2005/these-2592.pdf
http://archimer.ifremer.fr/doc/00000/2592/

Éditeur(s) : HAL CCSD Elsevier
Résumé : International audience
Sedimentary rocks and detrital minerals sample large areas of the continental crust, and they are increasingly seen as a reliable archive for its global evolution. This study presents two approaches to model the growth of the continental crust through the sedimentary archive. The first builds on the variations in U-Pb, Hf and O isotopes in global databases of detrital zircons. We show that uncertainty in the Hf isotope composition of the mantle reservoir from which new crust separated, in the 176Lu/177Hf ratio of that new crust, and in the contribution in the databases of zircons that experienced ancient Pb loss(es), adds some uncertainty to the individual Hf model ages, but not to the overall shape of the calculated continental growth curves. The second approach is based on the variation of Nd isotopes in 645 worldwide fine-grained continental sedimentary rocks with different deposition ages, which requires a correction of the bias induced by preferential erosion of younger rocks through an erosion parameter referred to as K. This dimensionless parameter relates the proportions of younger to older source rocks in the sediment, to the proportions of younger to older source rocks present in the crust from which the sediment was derived. We suggest that a Hadean/Archaean value of K = 1 (i.e., no preferential erosion), and that post-Archaean values of K = 4–6, may be reasonable for the global Earth system. Models built on the detrital zircon and the fine-grained sediment records independently suggest that at least 65% of the present volume of continental crust was established by 3 Ga. The continental crust has been generated continuously, but with a marked decrease in the growth rate at ~ 3 Ga. The period from > 4 Ga to ~ 3 Ga is characterised by relatively high net rates of continental growth (2.9–3.4 km3 yr− 1 on average), which are similar to the rates at which new crust is generated (and destroyed) at the present time. Net growth rates are much lower since 3 Ga (0.6–0.9 km3 yr− 1 on average), which can be attributed to higher rates of destruction of continental crust. The change in slope in the continental growth curve at ~ 3 Ga is taken to indicate a global change in the way bulk crust was generated and preserved, and this change has been linked to the onset of subduction-driven plate tectonics. At least 100% of the present volume of the continental crust has been destroyed and recycled back into the mantle since ~ 3 Ga, and this time marks a transition in the average composition of new continental crust. Continental crust generated before 3 Ga was on average mafic, dense, relatively thin (< 20 km) and therefore different from the calc-alkaline andesitic crust that dominates the continental record today. Continental crust that formed after 3 Ga gradually became more intermediate in composition, buoyant and thicker. The increase in crustal thickness is accompanied by increasing rates of crustal reworking and increasing input of sediment to the ocean. These changes may have been accommodated by a change in lithospheric strength at around 3 Ga, as it became strong enough to support high-relief crust. This time period therefore indicates when significant volumes of continental crust started to become emergent and were available for erosion and weathering, thus impacting on the composition of the atmosphere and the oceans.
ISSN: 0037-0738

hal-01622696
https://hal.archives-ouvertes.fr/hal-01622696
DOI : 10.1016/j.sedgeo.2017.06.001

Éditeur(s) : HAL CCSD Public Library of Science
Résumé : Simple sequence repeat (SSR) markers are widely used tools for inferences about genetic diversity, phylogeography and spatial genetic structure. Their applications assume that variation among alleles is essentially caused by an expansion or contraction of the number of repeats and that, accessorily, mutations in the target sequences follow the stepwise mutation model (SMM). Generally speaking, PCR amplicon sizes are used as direct indicators of the number of SSR repeats composing an allele with the data analysis either ignoring the extent of allele size differences or assuming that there is a direct correlation between differences in amplicon size and evolutionary distance. However, without precisely knowing the kind and distribution of polymorphism within an allele (SSR and the associated flanking region (FR) sequences), it is hard to say what kind of evolutionary message is conveyed by such a synthetic descriptor of polymorphism as DNA amplicon size. In this study, we sequenced several SSR alleles in multiple populations of three divergent tree genera and disentangled the types of polymorphisms contained in each portion of the DNA amplicon containing an SSR. The patterns of diversity provided by amplicon size variation, SSR variation itself, insertions/deletions (indels), and single nucleotide polymorphisms (SNPs) observed in the FRs were compared. Amplicon size variation largely reflected SSR repeat number. The amount of variation was as large in FRs as in the SSR itself. The former contributed significantly to the phylogenetic information and sometimes was the main source of differentiation among individuals and populations contained by FR and SSR regions of SSR markers. The presence of mutations occurring at different rates within a marker's sequence offers the opportunity to analyse evolutionary events occurring on various timescales, but at the same time calls for caution in the interpretation of SSR marker data when the distribution of within-locus polymorphism is not known.
ISSN: 1932-6203

hal-01190764
https://hal.archives-ouvertes.fr/hal-01190764
PRODINRA : 214530
PUBMED : 22808236
DOI : 10.1371/journal.pone.0040699

Éditeur(s) : Université du Littoral Côte d'Opale (Dunkerque)
Résumé : In the Bay of Biscay and Celtic Sea waters, hake is considered as genetically homogeneous and form a unique stock with identical meristic characteristics. The vertebral mean vary from 50,68 (vertebrae) during the first year of life to 51,11 (vertebrae) for adults. 0-group Merluccius display a rapid growth with significant interannual variability (0.71 mm.day-1 in 2001 versus 0.74 mm.day-1 in 2002). The spawning period occurs in April and the average length on the firth January following the hatching is of 17.3 cm (first seasonal check / increment on the sagittal otolith is located about 0.143 cm from the nucleus). At the end of the first year of life the average length is about 24 cm. The longevity of the species is important and growth parameters (L = 138,24 cm ; K = 0,132) explain natural mortality coefficient of M = 0.21. No difference for growth between male and female has been observed for this stock. The ratio total weight / eviscerated weight is equal to Fc = 1,086. Both male and female have same spatial and bathymetric distribution. Juveniles are found in deep waters (< 17 cm length) and move to coastal waters where they display an average length of (33 cm length). Longer individuals are found in nursery areas where they feed and grow then move to deeper areas to spawn. Nursery areas are numerous and located in the Celtic Sea and in the bay of Biscay. In the bay of Biscay, disturbances resulted from hydrondynamics affect the settlement on the nursery grounds particularly in the southern part of the bay. Diet composition varies with age. Juveniles feed mainly on crustaceans (Euphausia kroni). At the length of 23 cm, individuals feed strictly on fishes. Sizes of preys are proportional with sizes of predators and preys are composed mainly of species that feed and reproduce close to the bottom. Cannibalism has been observed for this specie, and increases with age notably in the northern part of the bay of Biscay and in the Celtic Sea. Main preys are 0-group juveniles. This specie is assumed to be a passive predator catching its preys when they come to feed in the bottom. Although they are punctual, these results have been obtained after studies on the evolution of sizes during the first year of life (daily increments measurements) and on the spawning period parameters (back-calculating). Average length on the firth January following hatching and the position of the first seasonal check on the otolith are based on observations of the sizes on the firth January following hatching (obtained with growth rates and spawning period parameters). The results are similar to those described in previous studies, nevertheless they have to be confirmed with growth and diet composition analyses. A long term study appear essential to determine biological and ecological parameters of this species for sustainable management of the stock.
Les alertes quant à la surexploitation du stock de merlu européen sont nombreuses et récurrentes. Seulement l'estimation du niveau réel de l'état du stock a toujours posé des problèmes du fait de certaines lacunes dans la connaissance de la biologie et de l'écologie de cette espèce. Dans les eaux du golfe de Gascogne et de la mer Celtique, le merlu européen fait partie de la même population génétique et possède les mêmes caractéristiques meristiques. Sa moyenne vertébrale est de 50,68 durant sa première année de vie et évolue pour se stabiliser à 51,11 chez l'adulte. La croissance du merlu durant sa première année de vie et très rapide mais présente une variabilité interannuelle significative (0,71 mm .J-1 en 2001 et 0,74 mm .J-1 en 2002). Sa période de ponte maximale se déroule au mois d'avril et sa longueur au premier janvier suivant sa naissance est de 17,3 cm (la première marque hivernale est positionnée à 0,143 cm du nucléus de la sagittae). Au terme de sa première année de vie le merlu atteint une longueur de 24 cm. Sa longévité est assez longue (23 ans environ) et ses paramètres de croissance (L = 138,24 cm ; K = 0,132) permettent d'estimer son coefficient de mortalité naturelle à M = 0,21. Il n'a pas été établi de croissance différentielle entre sexe chez le merlu dans ces eaux. Le rapport poids total / poids éviscéré a été estimé à Fc = 1,086. Mâles ou femelles, les merlus ont une même répartition spatiale et bathymétrique. En général, ils sont très profonds à leurs stades juvéniles (< 17 cm) et ils se dirigent vers les eaux côtières pour les atteindre à 33 cm de longueur environ. Au-delà de cette taille, les merlus se concentrent au niveau des zones de nourricerie pour s'y alimenter avant de rejoindre les eaux plus profondes pour y pondre. Les zones de nourricerie du merlu sont très nombreuses et sont localisées en mer Celtique et dans le golfe de Gascogne aussi bien dans sa partie sud que dans sa partie nord. Les perturbations importantes dans l'hydrodynamisme du golfe de Gascogne (upwelling) semble influencer les niveaux de colonisation des zones de nourricerie notamment celles situées dans la partie sud du golfe. Le régime alimentaire du merlu évolue avec l'âge. Juvénile, il se nourrit principalement de crustacés (Euphausia krohni) et devient ichtyophage exclusif dès 23 cm de longueur. Le cannibalisme est une réalité chez le merlu et les merlus-proies sont les juvéniles du groupe d'âge G-0. Il s'intensifie avec l'âge notamment dans la partie nord du golfe de Gascogne et en mer Celtique ; Il est très faible dans le sud du golfe de Gascogne. La taille des proies évolue avec la taille du merlu et les poissons proies sont en général des espèces qui vont se nourrir ou se reproduire sur le fond. Ce comportement alimentaire fait que le merlu est un prédateur peu actif et qui attaque ses proies lorsqu'elles viennent sur le fond. Ces résultats, bien qu'ils ne soient que ponctuels, ont été obtenus après avoir déterminé le schéma d'évolution de la longueur du merlu durant sa première année de vie (dénombrement des accroissement journaliers) et des paramètres de sa période de ponte (rétro-calcul).La taille au premier hiver et la position de la première marque hivernale ont été déterminées en relativisant nos observations à l'estimation de la longueur du merlu au premier janvier suivant sa naissance : celle-ci étant obtenue en utilisant le taux de croissance et les paramètres de la période de ponte. Bien que l'ensemble des résultats obtenus, en ce qui concerne la répartition spatiale et les données sur la période de ponte, soient généralement conformes à ceux décrits dans la littérature, il est nécessaire de confirmer ceux concernant la croissance et le régime alimentaire. Pour cela, une étude à plus long terme semble indispensable pour parvenir à bien maîtriser l'ensemble des paramètres de biologie et d'écologie du merlu permettant ainsi une meilleure gestion de son stock.

Droits : info:eu-repo/semantics/openAccess
http://archimer.ifremer.fr/doc/2004/these-1247.pdf
http://archimer.ifremer.fr/doc/00000/1247/

Éditeur(s) : Elsevier
Résumé : Evidence has accumulated that climate variability influences the state and functioning of marine ecosystems. At the same time increasing pressure from exploitation and other human activities has been shown to impact exploited and non-exploited species and potentially modify ecosystem structure. There has been a tendency among marine scientists to pose the question as a dichotomy, i.e., whether (1) "natural" climate variability or (2) fishery exploitation bears the primary responsibility for population declines in fish populations and the associated ecosystem changes. However, effects of both climate and exploitation are probably substantially involved in most cases. More importantly, climate and exploitation interact in their effects, such that climate may cause failure in a fishery management scheme but that fishery exploitation may also disrupt the ability of a resource population to withstand, or adjust to, climate changes. Here, we review how exploitation, by altering the structure of populations and ecosystems, can modify their ability to respond to climate. The demographic effects of fishing (removal of large-old individuals) can have substantial consequences on the capacity of populations to buffer climate variability through various pathways (direct demographic effects, effects on migration, parental effects). In a similar way, selection of population sub-units within metapopulations may also lead to a reduction in the capacity of populations to withstand climate variability and change. At the ecosystem level, reduced complexity by elimination of species, such as might occur by fishing, may be destabilizing and could lead to reduced resilience to perturbations. Differential exploitation of marine resources could also promote increased turnover rates in marine ecosystems, which would exacerbate the effects of environmental changes. Overall (and despite the specificities of local situations) reduction in marine diversity at the individual, population and ecosystem levels will likely lead to a reduction in the resilience and an increase in the response of populations and ecosystems to future climate variability and change. Future management schemes will have to consider the structure and functioning of populations and ecosystems in a wider sense in order to maximise the ability of marine fauna to adapt to future climates. (C) 2009 Elsevier B.V. All rights reserved.
Journal of Marine Systems (0924-7963) (Elsevier), 2010-02 , Vol. 79 , N. 3-4 , P. 403-417

Droits : 2009 Elsevier B.V. All rights reserved.
http://archimer.ifremer.fr/doc/2010/publication-7384.pdf
DOI:10.1016/j.jmarsys.2008.12.018
http://archimer.ifremer.fr/doc/00000/7384/

Éditeur(s) : HAL CCSD Wiley
Résumé : International audience
1. Despite considerable interest in the application of plant functional traits to questions of community assembly and ecosystem structure and function, there is no consensus on the appropriateness of sampling designs to obtain plot-level estimates in diverse plant communities. 2. We measured 10 plant functional traits describing leaf and stem morphology and ecophysiology for all trees in nine 1-ha plots in terra firme lowland tropical rain forests of French Guiana (N = 4709). 3.We calculated, by simulation, the mean and variance in trait values for each plot and each trait expected under seven sampling methods and a range of sampling intensities. Simulated sampling methods included a variety of spatial designs, as well as the application of existing data base values to all individuals of a given species. 4. For each trait in each plot, we defined a performance index for each sampling design as the proportion of resampling events that resulted in observed means within 5% of the true plot mean, and observed variance within 20%of the true plot variance. 5. The relative performance of sampling designs was consistent for estimations of means and variances. Data base use had consistently poor performance for most traits across all plots, whereas sampling one individual per species per plot resulted in relatively high performance. We found few differences among different spatial sampling strategies; however, for a given strategy, increased intensity of sampling resulted in markedly improved accuracy in estimates of trait mean and variance. 6. We also calculated the financial cost of each sampling design based on data from our 'every individual per plot' strategy and estimated the sampling and botanical effort required. The relative performance of designs was strongly positively correlated with relative financial cost, suggesting that sampling investment returns are relatively constant. 7. Our results suggest that trait sampling for many objectives in species-rich plant communities may require the considerable effort of sampling at least one individual of each species in each plot, and that investment in complete sampling, though great, may be worthwhile for at least some traits.
ISSN: 0269-8463

hal-00594009
https://hal.archives-ouvertes.fr/hal-00594009

Éditeur(s) : HAL CCSD Botanical Society of America
Résumé : Premise of the study : Wood density correlates with mechanical and physiological strategies of trees and is important for estimating global carbon stocks. Nonetheless, the relationship between branch and trunk xylem density has been poorly explored in neotropical trees. Here, we examine this relationship in trees from French Guiana and its variation among different families and sites, to improve the understanding of wood density in neotropical forests. Methods : Trunk and branch xylem densities were measured for 1909 trees in seven sites across French Guiana. A major-axis fit was performed to explore their general allometric relationship and its variation among different families and sites. Key results : Trunk xylem and branch xylem densities were significantly positively correlated, and their relationship explained 47% of the total variance. Trunk xylem was on average 9% denser than branch xylem. Family-level differences and interactions between family and site accounted for more than 40% of the total variance, whereas differences among sites explained little variation. Conclusions : Variation in xylem density within individual trees can be substantial, and the relationship between branch xylem and trunk xylem densities varies considerably among families and sites. As such, whole-tree biomass estimates based on non-destructive branch sampling should correct for both taxonomic and environmental factors. Furthermore, detailed estimates of the vertical distribution of wood density within individual trees are needed to determine the extent to which relying solely upon measures of trunk wood density may cause carbon stocks in tropical forests to be overestimated.
ISSN: 0002-9122

hal-01032401
https://hal.archives-ouvertes.fr/hal-01032401
DOI : 10.3732/ajb.1000034