Éditeur(s) : HAL CCSD American Association for the Advancement of Science
Résumé : International audience
Most eukaryotic organisms are arthropods. Yet, their diversity in rich terrestrial ecosystems is still unknown. Here we produce tangible estimates of the total species richness of arthropods in a tropical rainforest. Using a comprehensive range of structured protocols, we sampled the phylogenetic breadth of arthropod taxa from the soil to the forest canopy in the San Lorenzo forest, Panama. We collected 6144 arthropod species from 0.48 hectare and extrapolated total species richness to larger areas on the basis of competing models. The whole 6000-hectare forest reserve most likely sustains 25,000 arthropod species. Notably, just 1 hectare of rainforest yields >60% of the arthropod biodiversity held in the wider landscape. Models based on plant diversity fitted the accumulated species richness of both herbivore and nonherbivore taxa exceptionally well. This lends credence to global estimates of arthropod biodiversity developed from plant models.
ISSN: 0036-8075

hal-00817285
https://hal.archives-ouvertes.fr/hal-00817285
https://hal.archives-ouvertes.fr/hal-00817285/document
https://hal.archives-ouvertes.fr/hal-00817285/file/1226727Revisedtext.pdf
DOI : 10.1126/science.1226727

Éditeur(s) : HAL CCSD Wiley
Résumé : 1. Arthropods represent most of global biodiversity, with the highest diversity found in tropical rain forests. Nevertheless, we have a very incomplete understanding of how tropical arthropod communities are assembled. 2. We conducted a comprehensive mass sampling of arthropod communities within three major habitat types of lowland Amazonian rain forest, including terra firme clay, white-sand and seasonally flooded forests in Peru and French Guiana. We examined how taxonomic and functional composition (at the family level) differed across these habitat types in the two regions. 3. The overall arthropod community composition exhibited strong turnover among habitats and between regions. In particular, seasonally flooded forest habitats of both regions comprised unique assemblages. Overall, 17.7% (26 of 147) of arthropod families showed significant preferences for a particular habitat type. 4. We present a first reproducible arthropod functional classification among the 147 taxa based on similarity among 21 functional traits describing feeding source, major mouthparts and microhabitats inhabited by each taxon. We identified seven distinct functional groups whose relative abundance contrasted strongly across the three habitats, with sap and leaf feeders showing higher abundances in terra firme clay forest. 5. Our novel arthropod functional classification provides an important complement to link these contrasting patterns of composition to differences in forest functioning across geographical and environmental gradients. This study underlines that both environment and biogeographical processes are responsible for driving arthropod taxonomic composition while environmental filtering is the main driver of the variance in functional composition.
ISSN: 0021-8790

hal-01531619
https://hal.archives-ouvertes.fr/hal-01531619
PRODINRA : 349695
PUBMED : 26346553
DOI : 10.1111/1365-2656.12445

Éditeur(s) : HAL CCSD Elsevier
Résumé : International audience
ISSN: 0006-3207

hal-01090646
https://hal-agroparistech.archives-ouvertes.fr/hal-01090646
DOI : 10.1016/j.biocon.2010.07.034

Éditeur(s) : HAL CCSD
Résumé : A database providing information on mosquito specimens (Arthropoda: Diptera: Culicidae) collected in French Guiana is presented. Field collections were initiated in 2013 under the auspices of the CEnter for the study of Biodiversity in Amazonia (CEBA: http://www.labexceba.fr/en/). This study is part of an ongoing process aiming to understand the distribution of mosquitoes, including vector species, across French Guiana. Occurrences are recorded after each collecting trip in a database managed by the laboratory Evolution et Diversité Biologique (EDB), Toulouse, France. The dataset is updated monthly and is available online. Voucher specimens and their associated DNA are stored at the laboratory Ecologie des Forêts de Guyane (Ecofog), Kourou, French Guiana. The latest version of the dataset is accessible through EDB’s Integrated Publication Toolkit at http://130.120.204.55:8080/ipt/resource.do?r=mosquitoessur french_guiana or through the Global Biodiversity Information Facility data portal at http://www.gbif.org/dataset/5a8aa2ad-261c-4f61-a98e-26dd752fe1c5 It can also be viewed through the Guyanensis platform at http://guyanensis.ups-tlse.fr
ISSN: 1313-2989

hal-01601967
https://hal.archives-ouvertes.fr/hal-01601967
DOI : 10.3897/zookeys.532.6176
PRODINRA : 397188
PUBMED : 26692809

Résumé : Les objectifs de la missions étaient les suivants: PROJET TORTUES MARINES : (i) Prospection des îles de la côte ouest malgache pour récolter des échantillons génétiques de tortues verte et imbriquée dont l’analyse pourrait apporter des éléments essentiels dans la compréhension des liens qu’il existe entre le stock nord et sud de cette de cette zone ; (ii) finaliser la campagne de prélèvement génétique sur les immatures de tortues vertes présent dans le lagon de Juan de Nova PROJET ENTOMOLOGIE : (i) Inventaire de l’entomofaune et des autres arthropodes ; (ii) l’étude de l’écologie et de la biogéographie des îles éparses, à travers l’étude des phénomènes de spéciation et des réseaux trophiques ; (iii) constituer une collection de référence au Muséum d’Histoire Naturelle, support indispensable aux études ultérieures PROJET DE FILM : Afin de valoriser les activités scientifiques menées dans les îles Eparses depuis plusieurs années maintenant, Kélonia, le Muséum d’Histoire Naturel de La Réunion et l’Ifremer ont souhaité financer un film sur l’importance de la protection des îles éparses dans le cadre de la conservation de la biodiversité, conservation qui n’est possible que grâce à leur gestion par les Terre Australes et Antarctiques Françaises (TAAF).

Droits : 2007 Ifremer, Kélonia
http://archimer.ifremer.fr/doc/00244/35518/34023.pdf
http://archimer.ifremer.fr/doc/00244/35518/

Éditeur(s) : HAL CCSD Wiley-Blackwell
Résumé : International audience
1. Our understanding of the contribution of interspecific interactions to functional diversity in nature lags behind our knowledge of spatial and temporal patterns. Although two-species mutualisms are found in all types of ecosystems, the study of their ecological influences on other community members has mostly been limited to third species, while their influence on entire communities remains largely unexplored. 2. We hypothesized that mutualistic interactions between two respective ant species and an epiphyte mediate the biological traits composition of entire invertebrate communities that use the same host plant, thereby affecting food webs and functional diversity at the community level. 3. Aechmea mertensii (Bromeliaceae) is both a phytotelm ('plant-held water') and an ant-garden epiphyte. We sampled 111 bromeliads (111 aquatic invertebrate communities) associated with either the ant Pachycondyla goeldii or Camponotus femoratus. The relationships between ants, bromeliads and invertebrate abundance data were examined using a redundancy analysis. Biological traits information for invertebrates was structured using a fuzzy-coding technique, and a co-inertia analysis between traits and abundance data was used to interpret functional differences in bromeliad ecosystems. 4. The vegetative traits of A. mertensii depended on seed dispersion by C. femoratus and P. goeldii along a gradient of local conditions. The ant partner selected sets of invertebrates with traits that were best adapted to the bromeliads' morphology, and so the composition of the biological traits of invertebrate phytotelm communities depends on the identity of the ant partner. Biological traits suggest a bottom-up control of community structure in C. fenaoratus-associated phytotelmata and a greater structuring role for predatory invertebrates in P. goeldii-associated plants. 5. This study presents new information showing that two-species mutualisms affect the functional diversity of a much wider range of organisms. Most biological systems form complex networks where nodes (e.g. species) are more or less closely linked to each other, either directly or indirectly, through intermediate nodes. Our observations provide community-level information about biological interactions and functional diversity, and perspectives for further observations intended to examine whether large-scale changes in interacting species/community structure over broad geographical and anthropogenic gradients affect ecosystem functions.
Functional Ecology

hal-01096909
https://hal-agroparistech.archives-ouvertes.fr/hal-01096909
DOI : 10.1111/j.1365-2435-2011.01863.x

Éditeur(s) : HAL CCSD Springer Verlag
Résumé : International audience
Phthalates are common atmospheric contaminantsused in the plastic industry. Ants have been shown to constitutegood bioindicators of phthalate pollution. Hence,phthalates remain trapped on ant cuticles which are mostlycoated with long-chain hydrocarbons. In this study, we artificiallycontaminated Lasius niger ants with four phthalates:dibutyl phthalate (DBP), diisobutyl phthalate (DiBP), di(2-ethylhexyl) phthalate (DEHP), and benzyl butyl phthalate(BBP). The first three have previously been found on ants innature in Touraine (France), while the fourth has not. The fourphthalates disappeared rapidly (less than 5 days) from thecuticles of live ants. In contrast, on the cuticles of dead ants,DEHP quantities remained unchanged over time. These resultsindicate that phthalates are actively absorbed by thecuticles of live ants. Cuticular absorption of phthalates isnonspecific because eicosane, a nonnatural hydrocarbon onL. niger cuticle, was similarly absorbed. Ants are importantecological engineers and may serve as bioindicators of ecosystemhealth. We also suggest that ants and more generallyterrestrial arthropods may contribute to the removal ofphthalates from the local environment
ISSN: 0944-1344

hal-01081622
https://hal.archives-ouvertes.fr/hal-01081622
https://hal.archives-ouvertes.fr/hal-01081622/document
https://hal.archives-ouvertes.fr/hal-01081622/file/Lenoir-et-al-ESPR2014-10.1007_s11356-014-3272-2.pdf
DOI : 10.1007/s11356-014-3272-2

Éditeur(s) : Amer Soc Biochemistry Molecular Biology Inc
Résumé : Scleractinian corals are the most basal eumetazoan taxon and provide the biological and physical framework for coral reefs, which are among the most diverse of all ecosystems. Over the past three decades and coincident with climate change, these phototrophic symbiotic organisms have been subject to increasingly frequent and severe diseases, which are now geographically widespread and a major threat to these ecosystems. Although coral immunity has been the subject of increasing study, the available information remains fragmentary, especially with respect to coral antimicrobial responses. In this study, we characterized damicornin from Pocillopora damicornis, the first scleractinian antimicrobial peptide (AMP) to be reported. We found that its precursor has a segmented organization comprising a signal peptide, an acidic proregion, and the C-terminal AMP. The 40-residue AMP is cationic, C-terminally amidated, and characterized by the presence of six cysteine molecules joined by three intramolecular disulfide bridges. Its cysteine array is common to another AMP and toxins from cnidarians; this suggests a common ancestor, as has been proposed for AMPs and toxins from arthropods. Damicornin was active in vitro against Gram-positive bacteria and the fungus Fusarium oxysporum. Damicornin expression was studied using a combination of immunohistochemistry, reverse phase HPLC, and quantitative RT-PCR. Our data show that damicornin is constitutively transcribed in ectodermal granular cells, where it is stored, and further released in response to nonpathogenic immune challenge. Damicornin gene expression was repressed by the coral pathogen Vibrio coralliilyticus. This is the first evidence of AMP gene repression in a host-Vibrio interaction.
Journal Of Biological Chemistry (0021-9258) (Amer Soc Biochemistry Molecular Biology Inc), 2011-06 , Vol. 286 , N. 25 , P. 22688-22698

Droits : 2011 by The American Society for Biochemistry and Molecular Biology, Inc.
http://archimer.ifremer.fr/doc/00039/14996/12433.pdf
DOI:10.1074/jbc.M110.216358
http://archimer.ifremer.fr/doc/00039/14996/

Éditeur(s) : Taylor & Francis
Résumé : This study is an attempt to produce an assessment of the impact of shrimp aquaculture in the Mekong Delta (Viet Nam) on mangrove ecosystems. For this exercise we selected two sub-areas (Ca Mau and Tra Vinh provinces) encompassing a variety of land uses and ecological conditions. Twenty stations in Tra Vinh and 15 stations in Ca Mau have been surveyed several times from September 2000 to March 2002. Field investigations included mangrove soils studies, measurements of pH and salinity of the water, analysis of mangrove flora, and density and structure of the vegetation. Four Systeme Probatoire de l'Observation de la Terre (SPOT) scenes were used for the discrimination of mangrove types and for the delineation of landscape units. For the first time, five ecologically distinct landscape classes were identified and delineated. Their possible links with the farming and yields of high valued species of shrimps, especially the giant tiger shrimp (Penaeus monodon) destined for export markets, need further studies. Since 1965, about 30% of mangrove ecosystems have been lost in Ca Mau Province and more than 30% of present mangroves are replanted monospecific stands. To the best of our knowledge, this is the first study which demonstrates that, in spite of deep and ancient man interactions in the Mekong Delta, five ecologically distinct classes of land use can be defined. Satellite surveys confirm a clear distribution of landscape units with possible links with shrimp aquaculture potentialities.
International Journal of Remote Sensing (0143-1161) (Taylor & Francis), 2004-11 , Vol. 25 , N. 21 , P. 4795-4812

Droits : 2004 Taylor & Francis
http://archimer.ifremer.fr/doc/2004/publication-2303.pdf
DOI:10.1080/01431160412331270858
http://archimer.ifremer.fr/doc/00000/2303/

Résumé : Les fourmis sont des organismes cibles appropriés pour les études environnementales. Au cours de notre étude, nous nous sommes focalisés sur les fourmis de la litière via l'application de deux méthodes de récolte complémentaires selon le protocole all (ants of leaf litter). Dans les forêts naturelles de Guyane française, l'hétérogénéité environnementale et les perturbations structurent les communautés de fourmis de la litière en influençant la richesse, la diversité, l'abondance et la densité en espèces ainsi que les compositions taxonomique et fonctionnelle. Chaque type de formation végétale possède une communauté spécifique. La fragmentation et la conversion des forêts en plantations ont entrainé une altération plus ou moins profonde des communautés; cette variabilité est fonction du type d’agriculture et des espèces d'essences cultivées. Bien que l'altération des communautés se soit révélée maximale dans la plupart des monocultures, les plantations de cacaoyers ont un potentiel de conservation réel. Enfin, dans un contexte ou il est urgent de simplifier l'intégration des arthropodes dans les études de conservation, de contrôle et de suivi de la santé des écosystèmes terrestres, la méthode des réseaux de neurones est apparue comme un outil puissant pour mettre en évidence et analyser les patrons des communautés de fourmis.
Ants are reliable and relevant target organisms for environmental surveys. In our study, we focused on litter-dwelling ants through the use of two complementary sampling methods that were implemented according to the ants of leaf litter (all) protocol. In pristine forests of french guiana, leaf-litter ant communities are structured by environmental heterogeneity and natural perturbations - which influence species richness, diversity, abundance and density, as well as taxonomic and functional composition. This results in habitat-specific communities for each vegetal formation. Forest fragmentation and conversion into monocultures have led to a more or less deep alteration of the ant communities; this variability depends on the type of agricultural system and cultivated tree species. Although the intensity of community alteration peaked in tree monocultures, cocoa plantation exhibited a real potential for native species conservation. Finally, in the current context where simplifying the integration of arthropods into conservation programs as well as into surveys designed to monitor and manage the environment is critical, the use of neural networks appears to be a powerful tool for reliably highlighting and analyzing ant communities patterns.

http://www.theses.fr/2011AGUY0475/document

Éditeur(s) : HAL CCSD
Résumé : International audience
Reports on dengue morbidity in patients with sickle cell disease (SCD) are scarce. Dengue is arthropod-borne viroses due to dengue virus (DENV) and affects populations with a high prevalence of SCD; therefore, the risk of morbidity may be increased.To determine the impact of dengue infection in SCD patients, we reviewed medical records during a vast outbreak of dengue virus serotype-2 (DENV-2) in French Guiana in 2012-2013. We identified 10 cases of laboratory-confirmed dengue infection;these were classified into A, B and C categories based on levels of severity of dengue according to World Health Organisation (WHO). The average age of SCD patients was 18.2 years. 1, 6 and 3 cases were classified as A, B and C WHO severity criteria, respectively. All patients had an important decrease in haematocrit (while one of the WHO severity criteria is the increase of haematocrit). Only one patient had decreased platelet count. Seven cases had vaso-occlusive crisis (VOC), six patients developed acute hepatitis and eight had a simultaneous bacterial infection. Six of them needed a blood transfusion. The results suggested that the WHO severity laboratory criteria of hemoconcentration and platelet counts were not helpful in themanagement of SCD patients. The morbidity was mainly caused by specific complications of SCD, especially VOC, likely facilitated by vascular endothelial damages consecutive to dengue fever, severe haemolysis, hepatitis and bacterial co-infections.
Journal of Virology and Retrovirology

Droits : info:eu-repo/semantics/OpenAccess
inserm-01419456
http://www.hal.inserm.fr/inserm-01419456
http://www.hal.inserm.fr/inserm-01419456/document
http://www.hal.inserm.fr/inserm-01419456/file/2015%2C%20Mosnier%20-%20Dengue%20infection%20in%20sickle%20cell%20patients%20in%20French%20Guiana.pdf

Résumé : Les mécanismes à l’origine du maintien de la forte diversité locale des arbres des forêts tropicales humides constituent encore une énigme pour les scientifiques. Cette thèse a pour but d’étudier et de comprendre certains facteurs biotiques et abiotiques qui influencent l’assemblage des communautés des forêts tropicales humides d’Amazonie. A l’aide d’une approche empirique conduite dans les forêts tropicales de Guyane française et du Loreto au Pérou, de nombreuses expériences de terrain ont été mises en place dans le but d’apporter des éléments de réponse sur les mécanismes impliqués dans le maintien de la diversité des communautés d’arbres et d’insectes des forêts amazoniennes. Dans un premier temps, cette thèse permet de souligner l’importance des pressions biotiques exercées sur les communautés d’arbres. En effet, les communautés d’insectes peuvent influencer la composition des communautés d’arbres le long d’un gradient environnemental conduisant à des compromis entre la défense et la croissance. Ces compromis ou tradeoff permettent de maintenir la coexistence des espèces de forêts tropicales humides en favorisant la spécialisation des arbres à leur habitat (Chapitre 1, Annexe 3). Cependant, de nombreux facteurs peuvent engendrer des variations dans les compromis d’allocation exhibés par les plantes, ce qui peut compliquer la validité de ces résultats. Dans ce sens, nous avons souligné l’influence du rôle de la cascade trophique et l’existence chez certaines espèces d’arbres de stratégies d’évitement des insectes (Chapitre 2). Dans un second temps, nous avons montré que les filtres environnementaux et les distances géographiques favorisent un fort turnover de la composition des arthropodes des forêts tropicales (chapitre 3, Annexe 2). Les résultats de cette étude ont des implications fondamentales sur les mécanismes qui expliquent la structuration des communautés d’insectes herbivores. Nous avons souligné l’importance de l’interaction des communautés des insectes herbivores et de leurs plantes associées. De plus, des prédictions sont présentées sur le degré de spécialisation des insectes à leur plante-hôte (Annexe 3), notamment sur les implications possibles dans les compromis d’allocation chez les plantes. Finalement, des perspectives de recherche sont proposées en vue de poursuivre ces travaux de recherche, et notamment des extensions de mes expériences vers d’autres régions tropicales et tempérées et l’intégration de la phylogénie pour comprendre des mécanismes de coévolution entre communautés d’arbres et insectes. Nous proposons également une intégration des résultats de cette thèse dans les stratégies locales et régionales de conservation des forêts tropicales du bassin amazonien.
The mechanisms underlying the maintenance of local diversity of trees in tropical rainforests remain under debate. This dissertation aims to study and understand some biotic and abiotic factors that may influence both tree and insect community assembly in lowland tropical forests of Amazonia. I used an empirical approach to study communities of trees and insects in tropical forests of French Guiana and Peru, to address the extent to which insect herbivores contribute to the turnover of tree species across strong environmental gradients. In Chapter 1, I studied how herbivorous insect communities can influence the composition of tree communities along an environmental gradient by reinforcing tradeoffs between defense and growth that promote habitat specialization (Chapter 1, Appendix 3). The complicated variation in the patterns of growth and defense from this study led me to pursue further observations of an alternative plant defense strategy of time-avoidance of herbivores, which I examined in detail in Chapter 2. I found evidence for coordinated leaf production in some tree species that was consistent with the satiation of herbivores, suggesting that multiple interactions between plants and their herbivores may be responsible for patterns of habitat specialization in trees. In the second part of the dissertation, I examined insect herbivore communities in detail to test for turnover in species composition across geographic and environmental gradients. In Chapter 3, I present evidence for substantial beta-diversity in arthropod communities throughout lowland Amazonian forests. In the discussion I propose research perspectives to complete this research, including the extension of observations to compare tropical and temperate regions and the integration of molecular phylogenetics information to study coevolution of plant lineages and their insect herbivores. I conclude with suggestions for the integration of the results of this thesis in local and regional strategies for the preservation and conservation of tropical forests in the Amazon basin.

http://www.theses.fr/2013AGUY0582/document

Éditeur(s) : HAL CCSD Public Library of Science
Résumé : International audience
The common endosymbiotic Wolbachia bacteria influence arthropod hosts in multiple ways. They are mostly recognized for their manipulations of host reproduction, yet, more recent studies demonstrate that Wolbachia also impact host behavior, metabolic pathways and immunity. Besides their biological and evolutionary roles Wolbachia are new potential biological control agents for pest and vector management. Importantly, Wolbachia-based control strategies require controlled symbiont transfer between host species and predictable outcomes of novel Wolbachia-host associations. Theoretically, this artificial horizontal transfer could inflict genetic changes within transferred Wolbachia populations. This could be facilitated through de novo mutations in the novel recipient host or changes of haplotype frequencies of polymorphic Wolbachia populations when transferred from donor to recipient hosts. Here we show that Wolbachia resident in the European cherry fruit fly, Rhagoletis cerasi, exhibit ancestral and cryptic sequence polymorphism in three symbiont genes, which are exposed upon microinjection into the new hosts Drosophila simulans and Ceratitis capitata. Our analyses of Wolbachia in microinjected D. simulans over 150 generations after microinjection uncovered infections with multiple Wolbachia strains in trans-infected lines that had previously been typed as single infections. This confirms the persistence of low-titer Wolbachia strains in microinjection experiments that had previously escaped standard detection techniques. Our study demonstrates that infections by multiple Wolbachia strains can shift in prevalence after artificial host transfer driven by either stochastic or selective processes. Trans-infection of Wolbachia can claim fitness costs in new hosts and we speculate that these costs may have driven the shifts of Wolbachia strains that we saw in our model system.
ISSN: 1932-6203

hal-01544765
https://hal.archives-ouvertes.fr/hal-01544765
DOI : 10.1371/journal.pone.0082402

Éditeur(s) : HAL CCSD Wiley
Résumé : International audience
Wasp nests range from simple to complex structures made of paper or mud. Here, we show that a Neotropical wasp of the genus Nitela builds its nest entirely by weaving endophytic fungal hyphae and spider silk harvested from the leaves growing in the understory of the rain forest in French Guiana.
ISSN: 0006-3606

hal-01095857
https://hal-agroparistech.archives-ouvertes.fr/hal-01095857
DOI : 10.1111/j.1744-7429.2010.00645.x

Éditeur(s) : HAL CCSD
Résumé : International audience
ISSN: 1875-2535

hal-01084493
https://hal-agroparistech.archives-ouvertes.fr/hal-01084493
DOI : 10.1163/187525409X462412

Éditeur(s) : HAL CCSD Public Library of Science
Résumé : International audience
BACKGROUND: Dengue and malaria are two major public health concerns in tropical settings. Although the pathogeneses of these two arthropod-borne diseases differ, their clinical and biological presentations are unspecific. During dengue epidemics, several hundred patients with fever and diffuse pain are weekly admitted at the emergency room. It is difficult to discriminate them from patients presenting malaria attacks. Furthermore, it may be impossible to provide a parasitological microscopic examination for all patients. This study aimed to establish a diagnostic algorithm for communities where dengue fever and malaria occur at some frequency in adults. METHODOLOGY/PRINCIPAL FINDINGS: A sub-study using the control groups of a case-control study in French Guiana - originally designed to compare dengue and malaria co-infected cases to single infected cases - was performed between 2004 and 2010. In brief, 208 patients with malaria matched to 208 patients with dengue fever were compared in the present study. A predictive score of malaria versus dengue was established using .632 bootstrap procedures. Multivariate analysis showed that male gender, age, tachycardia, anemia, thrombocytopenia, and CRP>5 mg/l were independently associated with malaria. The predictive score using those variables had an AUC of 0.86 (95%CI: 0.82-0.89), and the CRP was the preponderant predictive factor. The sensitivity and specificity of CRP>5 mg/L to discriminate malaria from dengue were of 0.995 (95%CI: 0.991-1) and 0.35 (95%CI 0.32-0.39), respectively. CONCLUSIONS/SIGNIFICANCE: The clinical and biological score performed relatively well for discriminating cases of dengue versus malaria. Moreover, using only the CRP level turned to be a useful biomarker to discriminate feverish patients at low risk of malaria in an area where both infections exist. It would avoid more than 33% of unnecessary parasitological examinations with a very low risk of missing a malaria attack.
ISSN: 1935-2727

inserm-00872353
http://www.hal.inserm.fr/inserm-00872353
http://www.hal.inserm.fr/inserm-00872353/document
http://www.hal.inserm.fr/inserm-00872353/file/journal.pntd.0002420.pdf
DOI : 10.1371/journal.pntd.0002420