Éditeur(s) : HAL CCSD Elsevier
Résumé : International audience
ISSN: 0003-4975

hal-01563338
http://hal.univ-brest.fr/hal-01563338
DOI : 10.1016/j.athoracsur.2016.03.093
PUBMED : 27262916

Éditeur(s) : HAL CCSD Elsevier
Résumé : International audience
Aims: The aims of this study were to compare short-and long-term outcomes for clinical T2N0 oesophageal cancer with analysis of (i) primary surgery (S) versus neoadjuvant therapy plus surgery (NS), (ii) squamous cell carcinoma and adenocarcinoma subsets; and (iii) neoadjuvant chemoradiotherapy versus neoadjuvant chemotherapy.Methods: Data were collected from 30 European centres from 2000 to 2010. Among 2944 included patients, 355 patients (12.1%) had cT2N0 disease; 285 (S) and 70 (NS), were compared in terms of short-and long-term outcomes. Propensity score matching analyses were used to compensate for differences in baseline characteristics.Results: No significant differences between the groups were shown in terms of in hospital morbidity and mortality. Nodal disease was observed in 50% of S-group at the time of surgery, with 20% pN2/N3. Utilisation of neoadjuvant therapy was associated with significant tumour downstaging as reflected by increases in pT0, pN0 and pTNM stage 0 disease, this effect was further enhanced with neoadjuvant chemoradiotherapy. After adjustment on propensity score and confounding factors, for all patients and subset analysis of squamous cell and adenocarcinoma, neoadjuvant therapy had no significant effect upon survival or recurrence (overall, loco-regional, distant or mixed) compared to surgery alone. There were no significant differences between neoadjuvant chemotherapy and chemoradiotherapy in short-or long-term outcomes.Conclusion: The results of this study suggest that a surgery alone treatment approach should be recommended as the primary treatment approach for cT2N0 oesophageal cancer despite 50% of patients having nodal disease at the time of surgery.
ISSN: 0959-8049

hal-01666517
https://hal.univ-lorraine.fr/hal-01666517
DOI : 10.1016/j.ejca.2015.11.024

Éditeur(s) : HAL CCSD Public Library of Science
Résumé : International audience
ISSN: 1935-2727

hal-01447691
https://hal.archives-ouvertes.fr/hal-01447691
DOI : 10.1371/journal.pntd.0005254
PUBMED : 28081120

Résumé : Ce document constitue le bilan détaillé des résultats du projet DEDUCTION (2007-2010) , action par action. Le projet DEDUCTION "DEveloppement DUrable de la Crevetticulture, Traitement de l’Information et Observatoire du système en Nouvelle-Calédonie" est un projet de recherche et de développement en soutien à la filière crevetticole néo-calédonienne. C'est un programme très horizontal et pluridisciplinaire en poursuivant et développant les ouvertures scientifiques, techniques et thématiques établies à la faveur de DESANS. Son ossature s’articule autour de 4 actions : 3 actions de recherche et une action transversale devant faciliter les interfaces entre la recherche et sa finalité, entre les chercheurs et les partenaires locaux (GFA, Provinces, Gouvernement, Haut-Commissariat), en intégrant les résultats "ferme" à l'échelle de la filière. Une tâche de « Communication et de Transfert des connaissances » a été ajoutée en facteur commun des 4 actions scientifiques afin de répondre à la question fondamentale des échanges entre acteurs.

Droits : info:eu-repo/semantics/openAccess
http://archimer.ifremer.fr/doc/00199/31006/35663.pdf
http://archimer.ifremer.fr/doc/00199/31006/

Éditeur(s) : HAL CCSD Optical Society of America
Résumé : International audience
We present a theoretical simulation of the sarcomeric SHG intensity pattern (SHG-IP) that takes into account myofibrillar misalignment that is experimentally observed in SHG images of proteolysed muscles. The model predicts that myofibrillar displacement results in the conversion from one peak (1P) to two peaks (2P) sarcomeric SHG-IP in agreement with experimental results. This study suggests that sarcomeric SHG-IP is a powerful tool for mapping spatial myofibrillar displacement and its related excitation-contraction disruption that could occur during muscle physiological adaptation and disease.
ISSN: 1094-4087

hal-00820260
https://hal.archives-ouvertes.fr/hal-00820260
https://hal.archives-ouvertes.fr/hal-00820260/document
https://hal.archives-ouvertes.fr/hal-00820260/file/Studysur the_effect-accepted.pdf
DOI : 10.1364/OE.21.011404

Éditeur(s) : HAL CCSD Editions Quae
Résumé :  
absent
De l'observation des écosystèmes forestiers à l'information sur la forêt
Paris, France

hal-01032065
https://hal.archives-ouvertes.fr/hal-01032065

Droits : info:eu-repo/semantics/openAccess
http://archimer.ifremer.fr/doc/00040/15136/12475.pdf
http://archimer.ifremer.fr/doc/00040/15136/

Éditeur(s) : Actes du 24ème congrès de la World Aquaculture Society, 17ème congrès de l'European Aquaculture Society, Torremolinos, Espagne. 24 - 28 mai 1993
Résumé : Ce document est un rapport de mission commun des 44 chercheurs du groupe IFREMER ayant participé au 2ème congrès mixte de la World Aquaculture Society et de l'European Aquaculture Society qui s'est tenu à Torremolinos, en Espagne, du 24 au 28 mai 1993.

Droits : 1993 Ifremer
http://archimer.ifremer.fr/doc/00021/13207/10259.pdf
http://archimer.ifremer.fr/doc/00021/13207/

Éditeur(s) : HAL CCSD Landes Bioscience
Résumé : International audience
In 2008 we published the first set of guidelines for standardizing research in autophagy. Since then, research on this topic has continued to accelerate, and many new scientists have entered the field. Our knowledge base and relevant new technologies have also been expanding. Accordingly, it is important to update these guidelines for monitoring autophagy in different organisms. Various reviews have described the range of assays that have been used for this purpose. Nevertheless, there continues to be confusion regarding acceptable methods to measure autophagy, especially in multicellular eukaryotes. A key point that needs to be emphasized is that there is a difference between measurements that monitor the numbers or volume of autophagic elements (e.g., autophagosomes or autolysosomes) at any stage of the autophagic process vs. those that measure flux through the autophagy pathway (i.e., the complete process); thus, a block in macroautophagy that results in autophagosome accumulation needs to be differentiated from stimuli that result in increased autophagic activity, defined as increased autophagy induction coupled with increased delivery to, and degradation within, lysosomes (in most higher eukaryotes and some protists such as Dictyostelium) or the vacuole (in plants and fungi). In other words, it is especially important that investigators new to the field understand that the appearance of more autophagosomes does not necessarily equate with more autophagy. In fact, in many cases, autophagosomes accumulate because of a block in trafficking to lysosomes without a concomitant change in autophagosome biogenesis, whereas an increase in autolysosomes may reflect a reduction in degradative activity. Here, we present a set of guidelines for the selection and interpretation of methods for use by investigators who aim to examine macroautophagy and related processes, as well as for reviewers who need to provide realistic and reasonable critiques of papers that are focused on these processes. These guidelines are not meant to be a formulaic set of rules, because the appropriate assays depend in part on the question being asked and the system being used. In addition, we emphasize that no individual assay is guaranteed to be the most appropriate one in every situation, and we strongly recommend the use of multiple assays to monitor autophagy. In these guidelines, we consider these various methods of assessing autophagy and what information can, or cannot, be obtained from them. Finally, by discussing the merits and limits of particular autophagy assays, we hope to encourage technical innovation in the field.
Autophagy

hal-00735751
https://hal.archives-ouvertes.fr/hal-00735751
PUBMED : 22966490

Éditeur(s) : HAL CCSD Cambridge University Press (CUP)
Résumé :  
absent
ISSN: 0266-4674

hal-01032158
https://hal.archives-ouvertes.fr/hal-01032158
DOI : 10.1017/S0266467408005488

Éditeur(s) : Littoral 2004, Aberdeen, Scotland, UK, September 2004
Résumé : NAMIBIE (a french acronym for Integrated Multimedia Navigator Within Coastal Environment Data Bases) is a specific tool which first concept has been established by Ifremer and has been developped by an association between research in the field of coastal environment, innovation in the field of new information and communication technologies and services in the field of coastal integrated management and impact studies. The objectives of this tool are to facilitate research, direct access and display of relevant multimedia coastal integrated data and information according to an interactive, widely opened and playfull interface. The principles are based upon the fact that sustainable decision making needs an optimized unterstanding of coastal processes and better consultation between actors. In this purpose the development of the tool has considered that the end-user should and could be free to look after the data and the information he needs for a particular site or environment of interest and for a specific ICZM question according to easy navigation interfaces. That is why NAMIBIE concept is based upon : - a coherent 3D representation of the coasts (i.e. from catchement areas to continental shelf and deep sea), which considers a coherent DEM from land to sea and textural representation of the environment using satellite imagery textural displays, - a 3D navigator which allows the user to fly or dive over and within the coastal environment of interest, - a multimedia database (text, graphs, image, maps, geographic objects, modelling results...) specific to the territory of interest, - an interactive interface which helps in finding relevant information associated to specific questions. The technologies used include VRML data format, integration of standard GIS format (shp, grid, img) within the navigator, integration and display of modelling data. The production chain is realized in C++ and Java scripts allow dialogue between web pages and 3D windows. Applications are presented on the coasts of Languedoc Roussillon region (French Mediterranean border) in the framework of ICZM problematics

Droits : info:eu-repo/semantics/openAccess
http://archimer.ifremer.fr/doc/00089/20055/17696.pdf
http://archimer.ifremer.fr/doc/00089/20055/

Éditeur(s) : HAL CCSD
Résumé : absent
Séminaire forêts hétérogènes du GIP ECOFOR
NA, France

hal-01269340
https://hal.archives-ouvertes.fr/hal-01269340
PRODINRA : 331590

Éditeur(s) : HAL CCSD Optical Society Optical Society - SOA Publishing
Résumé : International audience
We show that the canonical single frequency sarcomeric SHG intensity pattern (SHG-IP) of control muscles is converted to double frequency sarcomeric SHG-IP in preserved mdx mouse gastrocnemius muscles in the vicinity of necrotic fibers. These double frequency sarcomeric SHG-IPs are often spatially correlated to double frequency sarcomeric two-photon excitation fluorescence (TPEF) emitted from Z-line and I-bands and to one centered spot SHG angular intensity pattern (SHG-AIP) suggesting that these patterns are signature of myofibrillar misalignement. This latter is confirmed with transmission electron microscopy (TEM). Moreover, a good spatial correlation between SHG signature of myofibrillar misalignment and triad reduction is established. Theoretical simulation of sarcomeric SHG-IP is used to demonstrate the correlation between change of SHG-IP and -AIP and myofibrillar misalignment. The extreme sensitivity of SHG microscopy to reveal the submicrometric organization of A-band thick filaments is highlighted. This report is a first step toward future studies aimed at establishing live SHG signature of myofibrillar misalignment involving excitation contraction defects due to muscle damage and disease.
ISSN: 2156-7085

hal-00952823
https://hal.archives-ouvertes.fr/hal-00952823
https://hal.archives-ouvertes.fr/hal-00952823/document
https://hal.archives-ouvertes.fr/hal-00952823/file/boe-5-3-858_002.pdf
DOI : 10.1364/BOE.5.000858

Éditeur(s) : The 11th Pacific Science Inter-Congress : Pacific Countries and their Ocean: Facing Local and Global Changes. March 2 - 6, 2009 Tahiti, French Polynesia
Résumé : New Caledonian shrimp farming feasibility studies started in the mid-seventies. Today ca. 2000 tons of Litopenaeus stylirostris, introduced in 1978, are semi-intensively produced each year. Since fifteen years, production has been impacted by two seasonal vibriosis, the “Summer” syndrome caused by Vibrio nigripulchritudo in relation to pond bottom and water column quality, and the “Winter Syndrome” caused by Vibrio penaeicida in relation to drastic temperature drops . With the ambition of elucidating processes possibly leading to mortality outbreaks in ponds, Ifremer has been conducting a research program since 2003 under the DESANS project (2003-2006) and the present DEDUCTION project, equally funded by the South and North Provinces and by the Government of New Caledonia. This program has been designed in a multidisciplinary approach integrating the suspected multifactorial origin of shrimp mortalities. Environment quality in ponds (either sediment or water column) plays a major role in the shrimp ecophysiological status, influencing sensitivity to pathogens . DEDUCTION focuses on a better knowledge of phytoplankton composition and biogeochemical fluxes at the sediment-water interface in ponds. Impact of farm discharges is also investigated. In addition, a more fundamental work is conducted to understand the pathogenicity mechanisms of these two Vibrio species but also to prevent the occurrence of viral infections through a RNA interference approach. Markers of the ecophysiological status of broodstock, larvae, post-larvae and reared animals, such as enzymes testifying from an oxidative stress, are developed. Gain of performance (resistance to pathogens and growth) were obtained on hybrids of the New Caledonian and introduced Hawaiian strains. Finally the database Stylog is exploited in view of discriminating “good” from “bad” rearings on the basis of relevant indicators.
Les études de faisabilité de l'aquaculture de crevettes ont débuté en Nouvelle-Calédonie dans les années soixante dix. Aujourd'hui environ 2000 tonnes de Litopenaeus stylirostris, introduite en 1978, sont produites chaque année de façon semi-intensive. Depuis quinze ans la production a été impactée par deux vibrioses : le syndrome d'été provoqué par Vibrio nigripulchritudo en relation avec la qualité de la tranche d'eau et des fonds de bassins et le syndrome d'hiver provoqué par Vibrio penaeicida en relation avec des chutes rapides de température. Dans le but d'élucider les processus entrainant des mortalités dans les élevages, Ifremer a conduit un programme de recherche depuis 2003 dans le cadre du projet DESANS (2003-2006) puis du présent projet DEDUCTION co-financé par les provinces Nord et Sud et le Gouvernement de la Nouvelle-Calédonie. Ce programme se construit dans une démarche multidisciplinaire intégrant les origines supposées multifactorielles des mortalités de crevettes. La qualité environnementale dans les bassins (les fonds et la colonne d'eau) joue un rôle majeur dans l'état physiologique des animaux, influençant leur sensibilité aux pathogènes. DEDUCTION se focalise sur une meilleure compréhension de la composition phytoplanctonique et des flux bio-géochimiques à l'interface eau/sédiment dans les bassins.L'impact des rejets des fermes est également étudié. En outre, un travail plus fondamental est conduit pour connaître les mécanismes de pathogénicité de ces deux espèces de Vibrio mais aussi pour prévenir les occurences d'infections virales par des approches ARN Interférence. Des marqueurs de l'état physiologique des oeufs, larves, post-larves et animaux en élevage, tels que les enzymes du stress oxydant sont utilisés.Les gains de performance (résistance aux pathogènes et croissance) sont obtenus sur des hybrides issus du croisement de la souche locale avec une souche hawaienne. Finalement la base de données STYLOG est exploitée dans le but de distinguer les bonnes des mauvaises pratiques sur la base d'indicateurs fiables.

Droits : info:eu-repo/semantics/openAccess
http://archimer.ifremer.fr/doc/00143/25438/23591.pdf
http://archimer.ifremer.fr/doc/00143/25438/

Résumé : L’Ifremer opère de façon coordonnée à l’échelle de l’ensemble du littoral métropolitain plusieurs réseaux de surveillance : le réseau de contrôle microbiologique (REMI), le réseau de surveillance du phytoplancton et des phycotoxines (REPHY), le réseau d’observation de la contamination chimique (ROCCH, ex-RNO) et le réseau de surveillance benthique (REBENT). Ils sont mis en oeuvre pour répondre aux objectifs environnementaux de la Directive Cadre sur l’Eau (DCE), aux obligations des Conventions régionales marines (OSPAR et Barcelone) et aux objectifs sanitaires réglementaires concernant le suivi de la salubrité des coquillages des zones de pêche et de production conchylicoles. La surveillance de la survie et de la croissance des mollusques est assurée par le Réseau Mollusques des Ressources Aquacoles (REMORA) qui est mis en place sur les 3 façades maritimes. Les réseaux de surveillance sont mis en oeuvre par les Laboratoires Environnement Ressources (LER) de l’Ifremer. Ils mettent également en oeuvre des réseaux de surveillance régionaux sur la côte d’Opale (SRN), le littoral normand (RHLN), le bassin d’Arcachon (ARCHYD) et les lagunes méditerranéennes (RSL-RLC), pour approfondir le diagnostic local. Ainsi, le dispositif de surveillance s’enrichit sur certains secteurs littoraux de résultats sur l’hydrologie soutenant l’évaluation de la qualité du milieu.

Droits : 2010 Ifremer
http://archimer.ifremer.fr/doc/00032/14346/11630.pdf
http://archimer.ifremer.fr/doc/00032/14346/

Éditeur(s) : HAL CCSD American Geophysical Union
Résumé : International audience
This study investigates three-dimensional flow modes of orogenic plateaus by means of physical modeling. Experiments consist of shortening two contiguous lithospheres of contrasting strength, one being a weak plateau-type lithosphere and the other a strong craton-type lithosphere. The lateral boundaries are either totally confined or allow escape toward a lateral foreland on one side. Two synconvergence flow regimes are distinguished, which are governed by the balance between the gravity potential and the strength of the plateau crust and the resistance of its lateral foreland. The first regime implies transversal (orogen-normal) injection of plateau lower crust into the collision zone as a result of confinement of the plateau by an increasingly stiffer lateral boundary. As a precursor mechanism to channel flow, transversal injection responds to downward thickening of the plateau crust that is forcedly extruded into the orogenic wedge. The second regime is that of collapse-driven lateral escape of the plateau. This regime is established after a threshold is attained in the interplate coupling in the collision zone, which allows the gravity potential of the plateau to overcome the resistance of its lateral boundary. Under the collapse-driven escape regime (orogen parallel), such as that governing Tibet during the last 13 Ma, most of the convergence in the plateau and the top and rear of the collisional wedge is transformed into lateral flow and extension.
ISSN: 0148-0227

hal-01229107
https://hal-univ-rennes1.archives-ouvertes.fr/hal-01229107
https://hal-univ-rennes1.archives-ouvertes.fr/hal-01229107/document
https://hal-univ-rennes1.archives-ouvertes.fr/hal-01229107/file/2015JB012110_revised.pdf
https://hal-univ-rennes1.archives-ouvertes.fr/hal-01229107/file/SupplementaryMaterial_final.pdf

Éditeur(s) : HAL CCSD Nature Publishing Group
Résumé : International audience
Polarization dependence second harmonic generation (P-SHG) microscopy is gaining increase popularity for in situ quantification of fibrillar protein architectures. In this report, we combine P-SHG microscopy, new linear least square (LLS) fitting and modeling to determine and convert the complex second-order non-linear optical anisotropy parameter ρ of several collagen rich tissues into a simple geometric organization of collagen fibrils. Modeling integrates a priori knowledge of polyhelical organization of collagen molecule polymers forming fibrils and bundles of fibrils as well as Poisson photonic shot noise of the detection system. The results, which accurately predict the known sub-microscopic hierarchical organization of collagen fibrils in several tissues, suggest that they can be subdivided into three classes according to their microscopic and macroscopic hierarchical organization of collagen fibrils. They also show, for the first time to our knowledge, intrahepatic spatial discrimination between genuine fibrotic and non-fibrotic vessels. CCl 4-treated livers are characterized by an increase in the percentage of fibrotic vessels and their remodeling involves peri-portal compaction and alignment of collagen fibrils that should contribute to portal hypertension. This integrated P-SHG image analysis method is a powerful tool that should open new avenue for the determination of pathophysiological and chemo-mechanical cues impacting collagen fibrils organization.
ISSN: 2045-2322

hal-01592192
https://hal.archives-ouvertes.fr/hal-01592192
https://hal.archives-ouvertes.fr/hal-01592192/document
https://hal.archives-ouvertes.fr/hal-01592192/file/s41598-017-12398-0.pdf
DOI : 10.1038/s41598-017-12398-0

Éditeur(s) : HAL CCSD Optical Society of America
Résumé : International audience
A linear least square (LLS) method is proposed to process polarization dependent SHG intensity analysis at pixel-resolution level in order to provide an analytic solution of nonlinear susceptibility χ(2) coefficients and of fibril orientation. This model is applicable to fibrils with identical orientation in the excitation volume. It has been validated on type I collagen fibrils from cell-free gel, tendon and extracellular matrix of F1 biliary epithelial cells. LLS is fast (a few hundred milliseconds for a 512 × 512 pixel image) and very easy to perform for non-expert in numerical signal processing. Theoretical simulation highlights the importance of signal to noise ratio for accurate determination of nonlinear susceptibility χ(2) coefficients. The results also suggest that, in addition to the peptide group, a second molecular nonlinear optical hyperpolarizability β contributes to the SHG signal. Finally from fibril orientation analysis, results show that F1 cells remodel extracellular matrix collagen fibrils by changing fibril orientation, which might have important physiological function in cell migration and communication.
ISSN: 1094-4087

hal-01139871
https://hal.archives-ouvertes.fr/hal-01139871
DOI : 10.1364/OE.23.013309
PUBMED : 26074581

Éditeur(s) : HAL CCSD EDP Sciences
Résumé : We propose and we justify a Monte-Carlo algorithm which solves a spatially homogeneous kinetic equation of Boltzmann type that models the fusion reaction between a deuterium ion and a tritium ion, and giving an α particle and a neutron. The proposed algorithm is validated with the use of explicit solutions of the kinetic model obtained by replacing the fusion cross-section by a Maxwellian cross section.
ISSN: 1270-900X

hal-00689821
https://hal.archives-ouvertes.fr/hal-00689821
https://hal.archives-ouvertes.fr/hal-00689821/document
https://hal.archives-ouvertes.fr/hal-00689821/file/proc_versionHAL.pdf

Éditeur(s) : HAL CCSD Biophysical Society
Résumé : International audience
SHG angular intensity pattern (SHG-AIP) of healthy and proteolysed muscle tissues are simulated and imaged here for the first time to our knowledge. The role of the spatial distribution of second-order nonlinear emitters on SHG-AIP is highlighted. SHG-AIP with two symmetrical spots is found to be a signature of healthy muscle whereas SHG-AIP with one centered spot in pathological mdx muscle is found to be a signature of myofibrillar disorder. We also show that SHG-AIP provides information on the three-dimensional structural organization of myofibrils in physiological and proteolysed muscle. Our results open an avenue for future studies aimed at unraveling more complex physiological and pathological fibrillar tissues organization.
ISSN: 0006-3495

hal-00825645
https://hal.archives-ouvertes.fr/hal-00825645
https://hal.archives-ouvertes.fr/hal-00825645/document
https://hal.archives-ouvertes.fr/hal-00825645/file/Theoretical-SHG-accepted.pdf
DOI : 10.1016/j.bpj.2013.02.047