Éditeur(s) : HAL CCSD Oxford University Press
Résumé : International audience
It was recently reported that the sizes of many mRNAs change when budding yeast cells exit mitosis and enter the meiotic differentiation pathway. These differences were attributed to length variations of their untranslated regions. The function of UTRs in protein translation is well established. However, the mechanism controlling the expression of distinct transcript isoforms during mitotic growth and meiotic development is unknown. In this study, we order developmentally regulated transcript isoforms according to their expression at specific stages during meiosis and gametogenesis, as compared to vegetative growth and starvation. We employ regulatory motif prediction, in vivo protein-DNA binding assays, genetic analyses and monitoring of epigenetic amino acid modification patterns to identify a novel role for Rpd3 and Ume6, two components of a histone deacetylase complex already known to repress early meiosis-specific genes in dividing cells, in mitotic repression of meiosis-specific transcript isoforms. Our findings classify developmental stage-specific early, middle and late meiotic transcript isoforms, and they point to a novel HDAC-dependent control mechanism for flexible transcript architecture during cell growth and differentiation. Since Rpd3 is highly conserved and ubiquitously expressed in many tissues, our results are likely relevant for development and disease in higher eukaryotes.
ISSN: 0305-1048

Droits : http://creativecommons.org/licenses/by/
hal-01122128
https://hal-univ-rennes1.archives-ouvertes.fr/hal-01122128
https://hal-univ-rennes1.archives-ouvertes.fr/hal-01122128/document
https://hal-univ-rennes1.archives-ouvertes.fr/hal-01122128/file/The%20conserved%20histone%20deacetylase.pdf
DOI : 10.1093/nar/gku1185

Éditeur(s) : ISTPM
Résumé : The study of the foraminifers contained in the samples collected by the "President-Theodore-Tissier" during its 1948 cruise enabled:1° to draw up the list of the genera and species dwelling in the north-eastern Atlantic Ocean;2° to notice that some of these species are widely distributed but that others are localized in these areas. Among them are:Quinqueloculina angulataQuinqueloculina dunkerquianaQuinqueloculina lataQuinqueloculina quadrangularisGaudryina rudisNonionella turgidaBolivina pseudo-plicataBolivina laevigataDiscorbis globularis var. anglicaDiscorbis millettiiDiscorbis nitida3° to clarify a few taxonomic points for some species;4° to classify the different stations according to the nature of the sea bed and to determine the foriminifer fauna living in each category;5° to establish the important role played by the substratum, not only because of its nature, but also because of the biocenose it is linked to.
L'étude des Foraminifères contenus dans les échantillons récoltes par le « President-Theodore-Tissier » au cours de sa campagne de 1948 a permis: 1° - d'établir la liste des genres et espèces vivant dans cette partie nord-orientale de l'Océan Atlantique; 2° de constater que certaines de ces formes ont une large distribution géographique mais que d'autres sont localisées dans ces régions. Parmi ces dernières on peut citer: Quinqueloculina angulata Quinqueloculina dunkerquiana Quinqueloculina lata Quinqueloculina quadrangularis Gaudryina rudis Nonionella turgida Bolivina pseudo-plicata Bolivina laevigata Discorbis globularis var. anglica Discorbis millettii Discorbis nitida 3° de préciser pour certaines espèces quelques points d'ordre systématique; 4° de classer les différentes stations suivant la nature du fond et de déterminer la composition de la faune des Foraminifères qui pullulent dans chacune des catégories; 5° de constater le rôle important joue par le substratum, non seulement par sa nature, mais aussi par la biocénose qui lui est étroitement liée. ATTENTION : Ce texte a été extrait du document par un processus automatique, non contrôlé, de reconnaissance optique de caractères (OCR). Il est donc susceptible de comporter des erreurs. En cas de doute, consultez le fichier PDF.
Revue des Travaux de l'Institut des Pêches Maritimes (0035-2276) (ISTPM), 1958-06 , Vol. 22 , N. 2 , P. 148-211

Droits : Ifremer
http://archimer.ifremer.fr/doc/1958/publication-4597.pdf
http://archimer.ifremer.fr/doc/00000/4597/

Éditeur(s) : HAL CCSD
Résumé : Afin de prévoir l'évolution des phénomènes naturels, sans discontinuité, des milliers d'images satellites alimentent les stations météorologiques. Ce flot permanent d'informations nécessite rapidité et rigueur d'analyse. Il est donc indispensable de mettre en place des systèmes capables de travailler nuit et jour afin de dresser des bilans exploitables par les chercheurs. Nous proposons dans cet article une méthode, basée sur des techniques d'investigation en reconnaissance des formes, telles que la segmentation et la classification. Ces techniques seront utilisées pour localiser et discriminer les masses nuageuses issues d'images captées par le satellite géostationnaire GOES. Le traitement des formes nuageuses trouvées se fait en utilisant des opérations, telles que la morphologie mathématique afin d'extraire des caractéristiques discriminantes. Pour l'interprétation des caractéristiques obtenues et pour effectuer la classification des nuages, nous utilisons des systèmes de classeurs.
Journées d'Études et de Recherche des Antilles et de la Guyane
Pointe-à-Pitre, Guadeloupe

hal-00637787
https://hal.archives-ouvertes.fr/hal-00637787

Éditeur(s) : HAL CCSD Elsevier
Résumé : International audience
Our goal was to determine if the US Environmental Relative Moldiness Index (ERMI) scale might have application in France. Twenty homes in Brittany, north western region of France were classified by inspection as "Moldy" or "Non-Moldy". Dust and air samples were collected (MiTest sampler or Coriolis sampler, respectively) from each home and analyzed by quantitative polymerase chain reaction (QPCR) for the 36 fungi that make-up the ERMI. Inspection and ERMI values provided a consistent assessment for 90% of the homes. Two homes originally classified as "Non-Moldy" were found to fit better into the "Moldy" category based on the QPCR analysis and the ERMI. Dust and air samples analyzed by QPCR provided similar fungal contamination assessments. In conclusion, a metric like the ERMI describes mold burdens in homes on a continuum, as opposed to the frequently used dichotomous approach (moldy vs. non-moldy). Although a larger, random national sampling of French homes is needed, these results suggest that these same 36 fungi may be useful in creating an ERMI for France.
ISSN: 1438-4639

inserm-00817898
http://www.hal.inserm.fr/inserm-00817898
http://www.hal.inserm.fr/inserm-00817898/document
http://www.hal.inserm.fr/inserm-00817898/file/Gangneux-Possible-Application-accepted.pdf
DOI : 10.1016/j.ijheh.2012.06.004

Résumé : Le projet de recherche actuel répond donc A un besoin d'enrichissement mutuel des approches biologiques et économiques face A un double enjeu : - socio-économique : la crise subie par un secteur représentant traditionnellement l'activité principale de certaines régions, - écologique : la survie de certaines espèces menacées par une pêche excessive, les deux enjeux étant directement liés dans de nombreux. L'idée est la suivante : à. partir des comportements de pêche observés au cours d'une année sur l'ensemble des bateaux de pêche inscrits A Boulogne-sur-Mer,essayer de définir des stratégies-types et d'isoler ainsi des groupes de bateaux aux comportements voisin. Cela devrait conduire A des distinctions de flottilles plus pertinentes que les classification : pêche industrielle, pêche au large, pêche côtière, petite pêche, qui tient compte des statuts juridiques et de la durée des marées, mais ne véhicule aucune information sur la dynamique des comportements et les choix stratégiques des navires. Avant d'aborder de façon plus détaillée l'analyse effectuée pour la mise en évidence et la compréhension des comportements de pêche, quelques éléments sur IFREMER et le SEM. Ensuite on présentera les activités de pêche A Boulogne-sur- Mer et leur évolution au cours des dernières années, la démarche suivie pour traiter les données, les résultats obtenus, les limites de l'approche. On verra enfin les développements ultérieurs possibles. [OCR NON CONTRÔLE]

Droits : info:eu-repo/semantics/openAccess
http://archimer.ifremer.fr/doc/1991/rapport-4216.pdf
http://archimer.ifremer.fr/doc/00000/4216/

Éditeur(s) : HAL CCSD Taylor & Francis
Résumé : International audience
The aim of this study was to examine the influence of exercise intensity and session duration on rating of perceived exertion (RPE) in adolescent Taekwondo (TKD) athletes. Twelve male youth competitive TKD athletes, aged between 13 and 18 years took part in this study. Training data (368 individual sessions) were collected during the 12 weeks of the precompetitive TKD season. Daily training load was calculated using the heart rate and the session-RPE. Exercise intensity was also classified in five zones [i.e. 5060%, 6170%, 7180%, 8190% and 91100% of maximal heart rate (HRmax)] and the time spent in each zone was expressed as percentage of total session time. The analysis of variance showed a significantmain effect for zone (pB0.001) with most of the time (69%) spent at intensities between 61 and 90% of HRmax, and only 10% spent above 91% (mean differences compared to the other zones ranging from 6.6% versus zone 1 to 14.8% versus zone 3; pB0.0001). The individual correlation between session-RPE and HR-based methods were moderate to large. The stepwise multiple regression showed that RPE was influenced mainly by the time spent in the high intensity zone that explained 22.1% of the variance in RPE. Session duration accounted for only an additional 3.2%. The results suggest the time spent at high-intensity (i.e. between 91 and 100% HRmax) and only marginally the session duration influences theRPE referred to the whole training session. This seems to confirm that the determination of the TL multiplying the RPE andsession duration is acceptable.
ISSN: 1746-1391

hal-01163939
https://hal.univ-antilles.fr/hal-01163939
DOI : 10.1080/17461391.2012.691115

Éditeur(s) : HAL CCSD
Résumé : International audience
Half away between edge detection and object recognition this study aim to characterize singularities in order to guide this two phases. The caracterization is obtained with a generalization of the Lipschitz exponents .for complex structures. This new characterization is called " maxima chain " and integrates the notion of value and spatial repartition of the maxima. The maxima chains are obtained with a multiscale decomposition using a Diadic Discrete Wavelet Transform (DDWT). We use the maxima of the gradient image computed with the wavelet details. The local maxima detect the position of irregular structures (edges) in the image (Canny algorithm). Then we characterize each maxima using their evolution across the different scales. A classification is made using the maxima chains. We integrate the classification to eliminate unwanted maxima (from noise or other objects) to drive the search of closed contours. In fact, there is a totally different signature from one maxima to another depending on the parameters of the objects : nature, shape, size, gray scale, texture, ...
RFIA Proceedings
Toulouse, France

hal-00602265
https://hal.archives-ouvertes.fr/hal-00602265

Résumé : Lorsqu'on s'occupe de la classification des Échinodermes et qu'on cherche pour leurs divisions des caractères naturels et précis, on est frappé des difficultés qu'on éprouve à 'comparer d'un groupe à l'autre les mêmes éléments de leur organisation si compliquée et si variée, et l'on s'étonne de trouver des points essentiels qui, aujourd'hui encore et après tant de travaux, ont besoin d'être éclaircis et de nouveau considérés. ,C'est dans l'espoir de pouvoir combler quelques-unes de ces lacunes qu'ont été entreprises les recherches consignées dans ce mémoire 1). Si, parmi les faits auxquels elles ont conduit, il y en a qui rendent moins difficiles à saisir certaines distinctions nécessaires à la parfaite connaissance des différents types de cette classe, qui mérite à tant d'égards d'être l'objet d'.études persévérantes, la science en est en premier lieu redevable à la subvention libérale, accordée, sur la demande de l'Académie des Sciences, par le Gouvernement et par la Diète pour l'exécution des planches, sans lesquelles il aurait été impossible de faire connaître avec clarté des détails de structure pour la plupart minutieux et assez difficiles à bien décrire.

Droits : info:eu-repo/semantics/openAccess
http://archimer.ifremer.fr/doc/00034/14488/11789.pdf
http://archimer.ifremer.fr/doc/00034/14488/

Éditeur(s) : HAL CCSD American Society for Microbiology
Résumé : International audience
Pneumocystis jirovecii pneumonia (PCP) is a life-threatening infection in immunocompromised patients. Quantitative real-time PCR (qPCR) is more sensitive than microscopic examination for the detection of P. jirovecii but also detects colonized patients. Hence, its positive predictive value (PPV) needs evaluation. In this 4-year prospective observational study, all immunocompromised patients with acute respiratory symptoms who were investigated for PCP were included, totaling 659 patients (814 bronchoalveolar lavage fluid samples). Patients with negative microscopy but positive qPCR were classified through medical chart review as having retained PCP, possible PCP, or colonization, and their clinical outcomes were compared to those of patients with microscopically proven PCP. Overall, 119 patients were included for analysis, of whom 35, 41, and 43 were classified as having retained PCP, possible PCP, and colonization, respectively. The 35 patients with retained PCP had clinical findings similar to those with microscopically proven PCP but lower fungal loads (P extless 0.001) and were mainly non-HIV-infected patients (P extless 0.05). Although the mean amplification threshold was higher in colonized patients, it was not possible to determine a discriminant qPCR cutoff. The PPV of qPCR in patients with negative microscopy were 29.4% and 63.8% when considering retained PCP and retained plus possible PCP, respectively. Patients with possible PCP had a higher mortality rate than patients with retained PCP or colonization (63% versus 3% and 16%, respectively); patients who died had not received co-trimoxazole. In conclusion, qPCR is a useful tool to diagnose PCP in non-HIV patients, and treatment might be better targeted through a multicomponent algorithm including both clinical/radiological parameters and qPCR results.
ISSN: 0095-1137

hal-01063906
https://hal.archives-ouvertes.fr/hal-01063906
DOI : 10.1128/JCM.01480-14
PUBMED : 25009050

Éditeur(s) : HAL CCSD Western Indian Ocean Marine Science Association
Résumé : International audience
Juan de Nova is one of the scattered islands known as Iles Eparses in the Mozambique Channel (Western Indian Ocean). Historically, they have been isolated from many anthropogenic influences which makes them ideal areas to study biodiversity for comparison with areas that are heavily impacted by urbanization and fishing. The programme BioReCie (Biodiversity, Resources and Conservation of Eparses Islands) undertook inventories of several marine groups, including echinoderms, which had hitherto not been assessed in Juan de Nova. A multidisciplinary team surveyed the reef slopes of the island using SCUBA to a depth of 25 m as well as the reef flats at low tide, collecting specimens and taking photos for identification. Sixty echinoderm species were found, with 51 occurring on the reef flats and in the lagoon and 22 on the reef slopes, comprising 21 species of Holothuroidea, 16 Ophiuroidea, 10 Echinoidea, 7 Asteroidea, and 6 Crinoidea. Commercial species of Holothuroidea, some of which are classified as endangered in the IUCN red list, i.e. Thelenota ananas and Holothuria nobilis, were present on the reef flats, reef slopes and in the lagoon of the island, indicating the value that protection has on biodiversity.
ISSN: 0856-860X

hal-01306709
http://hal.univ-reunion.fr/hal-01306709
http://hal.univ-reunion.fr/hal-01306709/document
http://hal.univ-reunion.fr/hal-01306709/file/A%20First%20Inventory%20of%20Echinodermata%20at%20Juan%20de%20Nova_hal.pdf

Éditeur(s) : Inter-Research
Résumé : The green turtle Chelonia mydas is classified as endangered because of global declines over the past few centuries due to human exploitation and habitat destruction, particularly the loss of nesting areas. We used the number of tracks as an indicator of breeding female abundance at their nesting sites to study the seasonality and trends of turtles breeding at 3 islands in the SW Indian Ocean: Europa, Tromelin and Grande Glorieuse, over 20 yr. On Tromelin, tracks were counted along the entire nesting beach, but on Europa and Grande Glorieuse counts were limited to a proportion of the island. Europa and Tromelin exhibited similar seasonal patterns, with a well-defined peak during the wet season (November–February), compared to a dry season peak for Grande Glorieuse (March–June). The main season was significantly longer on Grande Glorieuse (288 ± 43 d) than on Europa (218 ± 60 d), with Tromelin intermediate (252 ± 43 d). There was greater variation in the start of a season compared to the median and end at all sites throughout the study. Approximately 7178 ± 3053 (n = 19) tracks were recorded annually on the entire nesting beach on Tromelin, compared with 1480 ± 666 (n = 19) on 16% of nesting beaches on Grande Glorieuse and 1361 ± 903 (n = 23) on 26% of beaches on Europa. The number of tracks has increased significantly on Europa (3% yr–1) and Grande Glorieuse (6% yr–1). The increasing number of nesting turtles illustrates the effectiveness of conservation measures on sites formerly exploited by humans.
Endangered Species Research (1613-4796) (Inter-Research), 2007-08-15 , Vol. 3 , P. 217-227

Droits : 2007 Inter-Research
http://archimer.ifremer.fr/doc/00018/12917/9874.pdf
DOI:10.3354/esr003217
http://archimer.ifremer.fr/doc/00018/12917/

Éditeur(s) : HAL CCSD Frontiers Media
Résumé : International audience
To date 142 species have been described in the Vibrionaceae family of bacteria, classified into seven genera; Aliivibrio, Echinimonas, Enterovibrio, Grimontia, Photobacterium, Salinivibrio and Vibrio. As vibrios are widespread in marine environments and show versatile metabolisms and ecologies, these bacteria are recognized as one of the most diverse and important marine heterotrophic bacterial groups for elucidating the correlation between genome evolution and ecological adaptation. However, on the basis of 16S rRNA gene phylogeny, we could not find any robust monophyletic lineages in any of the known genera. We needed further attempts to reconstruct their evolutionary history based on multilocus sequence analysis (MLSA) and/or genome wide taxonomy of all the recognized species groups. In our previous report in 2007, we conducted the first broad multilocus sequence analysis (MLSA) to infer the evolutionary history of vibrios using nine housekeeping genes (the 16S rRNA gene, gapA, gyrB, ftsZ, mreB, pyrH, recA, rpoA, and topA), and we proposed 14 distinct clades in 58 species of Vibrionaceae. Due to the difficulty of designing universal primers that can amplify the genes for MLSA in every Vibrionaceae species, some clades had yet to be defined. In this study, we present a better picture of an updated molecular phylogeny for 86 described vibrio species and 10 genome sequenced Vibrionaceae strains, using 8 housekeeping gene sequences. This new study places special emphasis on (1) eight newly identified clades (Damselae, Mediterranei, Pectenicida, Phosphoreum, Profundum, Porteresiae, Rosenbergii, and Rumoiensis); (2) clades amended since the 2007 proposal with recently described new species; (3) orphan clades of genomospecies F6 and F10; (4) phylogenetic positions defined in 3 genome-sequenced strains (N418, EX25, and EJY3); and (5) description of V. tritonius sp. nov., which is a member of the “Porteresiae” clade.
ISSN: 1664-302X

hal-01546980
http://hal.upmc.fr/hal-01546980
DOI : 10.3389/fmicb.2013.00414

Éditeur(s) : Université de Paris
Résumé : Par les produits qu'elle fournit et le nombre d'hommes qu'elle occupe, la pêche de la morue tient, parmi les travaux auxquels s'adonnent les populations maritimes, une place prépondérante. Malgré cet intérêt et l'ancienneté de la découverte de Terre Neuve, les moeurs de la morue sont restées jusqu'à ces dernières années à peu près inconnues et les circonstances qui accompagnent sa migration demeurent encore obscures. La fabrication de l'huile de foie de morue est également mal établie et tout ce qui concerne la classification des huiles, leur différenciation et surtout l'identification des huiles natives n'a pas été jusqu'ici suffisamment étudié.

Droits : info:eu-repo/semantics/openAccess
http://archimer.ifremer.fr/doc/00032/14302/11581.pdf
http://archimer.ifremer.fr/doc/00032/14302/

Éditeur(s) : Elsevier
Résumé : Sea cruises were conducted for 57 days over 16 months to characterise pelagic fish aggregations around 2 moored fish aggregating devices (FADs) in Martinique (Lesser Antilles). Echosounder surveys run in a star pattern were used in conjunction with obliquely beamed sonar observations. An echo-integration-by-shoal algorithm was implemented to isolate pelagic fish shoals from sound scattering layers and to compute mean morphometric, positional and density parameters. Tree regressions were used to select and classify pelagic fish Target Strengths (TS), with reference to their spatial and temporal characteristics. The main type of pelagic fish aggregation was a large sub-surface aggregation. It was observed during all daytime periods within a radius of 400 m of the FAD. A smaller type of aggregation was observed closer to the surface and to the FAD in 65% of daytime periods. Large scattered fish were observed in 16% of daytime periods. At night, a medium-sized aggregation was detected in the sub-surface in 75% of night-time periods. The sizes of the fish inside the aggregations (determined from TS values) were lower in the small near-surface aggregation than in the large sub-surface aggregation. Mean packing densities of sub-surface medium fish and near-surface small fish aggregations (determined from TS and shoal acoustic density) were respectively 0.2 fish per m3 and 1.3 fish per m3. The acoustic methodology and results are discussed with reference to the characteristics and performance of the echosounder and to the spatial structure of pelagic fish aggregations around moored FADs in Martinique.
Fisheries Research (0165-7836) (Elsevier), 2006-06 , Vol. 82 , N. 1-3 , P. 162-175

Droits : Elsevier 2006
http://archimer.ifremer.fr/doc/2006/publication-1734.pdf
DOI:10.1016/j.fishres.2006.06.025
http://archimer.ifremer.fr/doc/00000/1734/

Éditeur(s) : HAL CCSD Elsevier
Résumé : International audience
During the treatment process of drinking water, disinfectants (chlorine, ozone, chlorine dioxide) react on water containing organic matter and bromide to produce disinfection by-products at trace levels. Among them, five of the nine existing halo-acetic acids (HAAs) are commonly found in drinking water (monochloroacetic acid (MCAA), dichloroacetic acid (DCAA), monobromoacetic acid (MBAA), dibromoacetic acid (DBAA), and Trichloroacetic acid (TCAA)), including four classified in the 2B IARC group of potential carcinogens (BCAA, DBAA, DCAA, TCAA). With respect to drinking water quality, guidelines are proposed by WHO (2006) and water quality standards are imposed in many countries such as less than 100 μg/L for the sum of the five HAAs by US EPA (1998) and Canadian Health Department (2008). For this purpose, two analytical methods are commonly used, GC/MS with derivatization and LC/MS, UV or conductivity. A new method, based on two-dimensional ion chromatography (IC 2D) with suppressed conductivity is proposed. This method presents the main advantage of offering a quick implementation compared to GC or LC methods: direct injection, slight maintenance, lower cost of investment, by leading to good performances (specificity and sensitivity). The use of two different selectivity columns, and the fractionation on the first dimension canceling interferences, improves the specificity. The sensitivity is enabled by interfacing a preconcentration column between the two different internal diameter columns. The analytical conditions are optimized for the analysis of nine HAAs. The performances of the method are evaluated. The optimized method applied to natural water samples demonstrates its ability to quantify HAAs at trace levels in drinking water.
Microchemical Journal

hal-00877151
https://hal-univ-rennes1.archives-ouvertes.fr/hal-00877151
https://hal-univ-rennes1.archives-ouvertes.fr/hal-00877151/document
DOI : 10.1016/j.microc.2013.07.012

Éditeur(s) : HAL CCSD Public Library of Science
Résumé : International audience
BACKGROUND: The use of pesticides and the related environmental contaminations can lead to human exposure to various molecules. In early-life, such exposures could be responsible for adverse developmental effects. However, human health risks associated with exposure to complex mixtures are currently under-explored. OBJECTIVE: THIS PROJECT AIMS AT ANSWERING THE FOLLOWING QUESTIONS: What is the influence of exposures to multiple pesticides on the metabolome? What mechanistic pathways could be involved in the metabolic changes observed? METHODS: Based on the PELAGIE cohort (Brittany, France), 83 pregnant women who provided a urine sample in early pregnancy, were classified in 3 groups according to the surface of land dedicated to agricultural cereal activities in their town of residence. Nuclear magnetic resonance-based metabolomics analyses were performed on urine samples. Partial Least Squares Regression-Discriminant Analysis (PLS-DA) and polytomous regressions were used to separate the urinary metabolic profiles from the 3 exposure groups after adjusting for potential confounders. RESULTS: The 3 groups of exposure were correctly separated with a PLS-DA model after implementing an orthogonal signal correction with pareto standardizations (R2 = 90.7% and Q2 = 0.53). After adjusting for maternal age, parity, body mass index and smoking habits, the most statistically significant changes were observed for glycine, threonine, lactate and glycerophosphocholine (upward trend), and for citrate (downward trend). CONCLUSION: This work suggests that an exposure to complex pesticide mixtures induces modifications of metabolic fingerprints. It can be hypothesized from identified discriminating metabolites that the pesticide mixtures could increase oxidative stress and disturb energy metabolism.
ISSN: 1932-6203

inserm-00863407
http://www.hal.inserm.fr/inserm-00863407
http://www.hal.inserm.fr/inserm-00863407/document
http://www.hal.inserm.fr/inserm-00863407/file/journal.pone.0064433.pdf
PUBMED : 23704985

Éditeur(s) : HAL CCSD Elsevier
Résumé : International audience
Glioblastoma (GB), the most frequent primary tumor of the central nervous system, remains one of the most lethal human cancers despite intensive researches. Current paradigm in the study of GB has been focused on inter-patient variability and on trying to isolate new classification elements or prognostic factors. Here, using ICPL, a technique for protein relative quantification by mass spectrometry, we investigated protein expression between the four regions of GB on clinically relevant biopsies from 5 patients. We identified 584 non-redundant proteins and 31 proteins were found to be up-regulated in the tumor region compared to the peri-tumoral brain tissue, among which, 24 proteins belong to an interaction network linked to 4 biological processes. The core of this network is mainly constituted of interactions between beta-actin (ACTB) with heat shock proteins (HSP90AA1, HSPA8) and 14-3-3 proteins (YWHAZ, YWHAG, YWHAB). A cluster of three isoforms of the sodium pump α-subunit (ATP1A1, ATP1A2, ATP1A3) was also identified outside this network. The differential expression observed for ACTB and 14-3-3γ was further validated by western blot and/or immunohistochemistry. Our study confirms the identity of previously proposed molecular targets, highlights several functional processes altered in GB such as energy metabolism and synaptic transmission and could thus provide added value to new therapeutic trails.
ISSN: 1874-3919

hal-00877756
https://hal.archives-ouvertes.fr/hal-00877756
DOI : 10.1016/j.jprot.2012.04.034
PUBMED : 22575386

Éditeur(s) : HAL CCSD Society for the Study of Reproduction
Résumé : International audience
Matrix-assisted laser desorption/ionization (MALDI) tissue imaging mass spectrometry is particularly promising among the numerous applications of mass spectrometry. It is used for probing and analyzing the spatial arrangement of a wide range of molecules, including proteins, peptides, lipids, drugs, and metabolites, directly in thin slices of tissue. In the field of proteomics, the technology avoids tedious and time-consuming extraction and fractionation steps classically required for sample analysis. MALDI imaging mass spectrometry is increasingly recognized as a powerful method for clinical proteomics, particularly in cancer research. The technology has particular potential for the discovery of new tissue biomarker candidates, classification of tumors, early diagnosis or prognosis, elucidating pathogenesis pathways, and therapy monitoring. Over recent years, MALDI imaging mass spectrometry has been used for molecular profiling and imaging directly in male and female reproductive tissues. This review will consider some of the recent publications in the field, addressing a range of issues covering embryo development, gene expression product profiling during gametogenesis, and seeking and identifying biomarkers of reproductive cancers. The wealth of advances in mass spectrometry imaging will inevitably attract biologists and clinicians as the advantages and power of this technology become more widely known. This review will also discuss bottlenecks and the many technical issues that remain to be resolved before laboratories in the field can adopt the technology. We foresee that MALDI imaging mass spectrometry will have a major impact in reproductive research by opening new avenues to the understanding of various molecular mechanisms and the diagnosis of reproductive pathologies.
ISSN: 0006-3363

hal-00877851
https://hal.archives-ouvertes.fr/hal-00877851
DOI : 10.1095/biolreprod.111.094896
PUBMED : 22156474

Éditeur(s) : Wiley-blackwell Publishing, Inc
Résumé : We present the results from processing and interpreting five lines from the 1992 Erable multichannel seismic reflection experiment extending from the southeastern margin of Flemish Cap into the northern Newfoundland Basin. These profiles reveal significant along strike variations in the rifting styles experienced by Flemish Cap. In the southwest, a 100-km-wide transition zone is identified between thinned continental crust and thin oceanic crust. Similar to the conjugate Galicia Bank and Iberian margins, this transition zone contains a section of deep basement adjacent to a series of shallower ridges and is interpreted as exhumed serpentinized mantle. Along strike towards the northeast, this transition zone pinches out completely within 100 km and is replaced by thin oceanic crust directly adjacent to thinned continental crust. By interpreting nearby seismic profiles and profiles on the conjugate margins using the same classification criteria, we construct regional maps of the distribution of crustal domains on both sides of the North Atlantic. These maps reveal significant variations in rifting style on the conjugate margins and along strike of each margin and also highlight the role of ancient transfer zones in compartmentalizing these rifting variations into four distinct regions. We propose that the limited localization of shallow topographically high serpentinized peridotite ridges on the Newfoundland-Iberia and Flemish Cap-Galicia Bank conjugate margins, was directly related to an increase in the rate of extension following the separation of Flemish Cap and Galicia Bank which exhumed deeper, less serpentinized mantle.
Geophysical Journal International (0956-540X) (Wiley-blackwell Publishing, Inc), 2010-03 , Vol. 180 , N. 3 , P. 976-U4

Droits : 2010 RAS
http://archimer.ifremer.fr/doc/00001/11202/7876.pdf
DOI:10.1111/j.1365-246X.2009.04477.x
http://archimer.ifremer.fr/doc/00001/11202/

Éditeur(s) : HAL CCSD Association de Géographes Français
Résumé : International audience
The international symposium on Urban Growth and Natural Hazards, in particular in Developing Countries was held in the Université Blaise-Pascal, Clermont-Ferrand (France) on December 2 and 3, 1994. This meeting brought together about 70 researchers, Ph. D. students, and professionals from France and 12 other countries, representing universities, research agencies, as well as government and private companies. The symposium aimed to gather specialists who are engaged and acting in programmes on hazard appraisal, risk prevention and urban management in the city environment. These people compared experiences acquired in two different fields: on the one hand urban growth and its consequences, and on the other hand, natural hazards and their effects, in particular in developing countries.From the 41 contributions given at the symposium, 4 papers were selected by the editorial board of this Bulletin de l'Association de Géographes Français for this special issue, which includes three chapters corresponding to the three sessions of the symposium: (1) Natural hazards and environmental factors : inventory, diagnostic and classification, (2) Vulnerability of urban society and environment : concepts, approaches, and classification, (3) Tools for urban risk prevention and risk management in case of crisis and catastrophes.
Ce numéro du Bulletin de l'Association de Géographes Français résume les résultats des tables rondes et de 41 contributions de chercheurs français et étrangers au colloque international «Croissance urbaine et risques naturels dans les pays en développement» qui s'est déroulé à l'Université Blaise-Pascal, Clermont-Ferrand, les 2 et 3 décembre 1994. L'objectif général du colloque était de rassembler les diverses compétences existant en France et à l'étranger dans le domaine de l'évaluation et de la gestion des risques naturels et de confronter les expériences acquises à l'interface entre les villes en croissance et le champ d'action des phénomènes naturels créateurs de risques. Le colloque a donc réuni des chercheurs, des doctorants et des praticiens de 12 pays, chargés de prévention, de gestion des risques naturels et de gestion urbaine, principalement dans les grandes villes des pays en développement (P.E.D.).Les contributions ont été regroupées, exposées et discutées lors du colloque sous forme de 3 thèmes : (1) Les phénomènes naturels créateurs de dommages (= menaces) : diagnostic, inventaire et typologie, (2) Les vulnérabilités des sociétés et des espaces urbanisés : concepts, typologie et modes d'analyse, (3) Les outils d'aide à la prévention et à la gestion du risque en milieu urbain. Certaines d'entre elles ont fait l'objet des quatre articles de ce Bulletin de l'Association de Géographes Français.
Colloque international : "Croissance urbaine et risques naturels dans les pays en développement"
Clermont-Ferrand, Université Blaise Pascal, France

ISBN : 0004-5322
hal-01165290
https://hal.archives-ouvertes.fr/hal-01165290