Éditeur(s) : Gauthier-Villars
Résumé : Simple thermodynamics calculations are applied to processes of a rising plume within an environment of an upward directed gradient of viscosity in the uppermost mantle. Four stages of the development of passive margins are described. Following an initial regional uplift strong lateral movements are induced at asthenospheric levels, stretching the overlying lithosphere. In the second stage plume material also enters the low viscosity lower crust, replacing continental material and forming a new, higher crust-mantle boundary by gravitational differentiation. The inital phase lag between the laterally intruding plume material and the rest of the lithosphere disappears once the continent is rifted and sea floor spreading is established (= stage 3). Finally, at stage 4, quiet subsidence, controlled by contraction, sedimentation, and possible phase changes in the lower crust, is established. The sequence described is in accordance with many observations of geology and geophysics. Rising plumes and sinking slabs are considered equivalent expressions of the heat engine earth which determines global tectonics.
Oceanologica Acta, Special issue (0399-1784) (Gauthier-Villars), 1981

Droits : info:eu-repo/semantics/openAccess
http://archimer.ifremer.fr/doc/00245/35658/34167.pdf
http://archimer.ifremer.fr/doc/00245/35658/

Éditeur(s) : Nature
Résumé : Mud volcanism is an important natural source of the greenhouse gas methane to the hydrosphere and atmosphere(1,2). Recent investigations show that the number of active submarine mud volcanoes might be much higher than anticipated ( for example, see refs 3 - 5), and that gas emitted from deep-sea seeps might reach the upper mixed ocean(6-8). Unfortunately, global methane emission from active submarine mud volcanoes cannot be quantified because their number and gas release are unknown(9). It is also unclear how efficiently methane-oxidizing microorganisms remove methane. Here we investigate the methane-emitting Haakon Mosby Mud Volcano (HMMV, Barents Sea, 72 degrees N, 14 degrees 44' E; 1,250 m water depth) to provide quantitative estimates of the in situ composition, distribution and activity of methanotrophs in relation to gas emission. The HMMV hosts three key communities: aerobic methanotrophic bacteria (Methylococcales), anaerobic methanotrophic archaea (ANME-2) thriving below siboglinid tubeworms, and a previously undescribed clade of archaea (ANME-3) associated with bacterial mats. We found that the upward flow of sulphate- and oxygen-free mud volcano fluids restricts the availability of these electron acceptors for methane oxidation, and hence the habitat range of methanotrophs. This mechanism limits the capacity of the microbial methane filter at active marine mud volcanoes to <40% of the total flux.
Nature (depuis 1997) (0028-0836) (Nature), 2006-10 , Vol. 443 , N. 7113 , P. 854-858

Droits : 2006 Nature Publishing Group
http://archimer.ifremer.fr/doc/2006/publication-4514.pdf
DOI:10.1038/nature05227
http://archimer.ifremer.fr/doc/00000/4514/

Éditeur(s) : Blackwell science
Résumé : This paper provides an exploratory analysis aiming to seek whether the colour of environmental noise theory could help in understanding the intriguing reproductive strategy of Atlantic bluefin tuna (BFT). A frequency-based approach based on spectral exponents, f(beta) with beta < 0, is chosen and applied on 10 biogeographical provinces covering the North Atlantic. The major BFT spawning area, i.e. the Mediterranean Sea, was the only one to display a pink power spectrum, whereas open ocean regions displayed more reddened fluctuations, i.e. greater variance at low frequencies. Environmental noise in the Mediterranean could, thus, offer more favourable characteristics on the long-term than the open ocean. The implications of these findings are discussed in regards to medium and long (possibly evolutionary) time scales.
Fisheries Oceanography (1054-6006) (Blackwell science), 2007-03 , Vol. 16 , N. 2 , P. 202-206

Droits : 2007 Blackwell science
http://archimer.ifremer.fr/doc/2007/publication-2437.pdf
DOI:10.1111/j.1365-2419.2006.00424.x
http://archimer.ifremer.fr/doc/00000/2437/

Résumé : In the beginning, the coastal fleet of the Bigouden region fished by trawling and was completely focused on exploiting lobster and hake. It is a traditional business that expanded between the last war and the 70s, reaching its peak between 1975 and 1980. The best image to give an idea of this fleet is that of a "bucket waterwheel" endlessly discharging its catch in the four ports of the Guilvinec district. Where there is a seafaring business in Guilvinec, Loctudy and Saint-Guénolé, there is a complementarity that is expressed in two ways: - coastal fishing constitutes a very noticeable and regular supplement to production, since sales happen daily and only bad winter weather causes a relative decrease in the supplement. - coastal fishing gives a flattering image of the port based on the display of high-quality leading market products: daily live lobster, hake and angler. In this study, we have above all tried to evaluate the situation of the Bigouden coastal system as an entity, while including in the same analysis the species, fleets and the exploitation of the resource. For the main species, a balance sheet is proposed. It covers the lobster, hake and angler separately. Lobster, because of its small amount of movement, can be considered as being an isolated stock, while fish belong to much more wide-ranging groups. The lobster has gained from various increases in meshing that have been imposed since 1971, even if these have not always been strictly applied. One of the results of this management by means of meshing is a "depreciation" of the variations of daily yields around an average value of 100kg. For the hake, the dominant trait is an increase in yields in the last few years in accordance with an improvement of the situation in the Gulf of Gascony due to a generalised decrease of effort and a modification of the exploitation profile since the appearance of boulters and straight nets. Whereas the situation for lobster fisheries, as regards the hake's marketable size, did not improve, since the demand for small hake still exists, we have nonetheless been witnessing for about ten years a slow diversification of the fleets' business activities, which is much more in line with sequential hake fisheries' logical outline of exploitation. When lobster yields decrease, one part of the fleet focuses on the fish at the edges of the lobster fisheries. For the angler, we see rather similar phenomena and a significant increase in unloading of the two species by lobster fishermen due also to a widening of their fishing areas. On the lobster mudflats themselves, there is no notable change in exploitation in the historical records. All studies of coastal fishing always come up against the problem of acquiring statistical data and the Bigouden region is no exception to the rule. To improve this situation, in 1987 and 1988 two items were chosen: - a better awareness of the activity and of the areas frequented, by survey during unloading in Guilvinec and in Loctudy. - a detailed description of the haul by species, by sampling, in Loctudy, on two-thirds of coastal sales, between December 1987 and November 1988. For this reason, analysis of fleets by type is still cursory and covers only one year. The appearance of new trends towards fish can only be described qualitatively, since there is no historical record. The classification of boats was done by analysing data furnished by the auctions. This first assessment is not necessarily reliable, above all because of the auctions' inaccurate description of the hauls, a deficiency that we have tried to overcome by surveying in Loctudy. In conclusion, we are witnessing in the Bigouden region, as in other entities, a rebalancing between traditional business activities and modern trends. Lobster fishing, almost exclusive before the 1980s, is tending to decrease for two main reasons: - a generalised decrease in activity in the lobster fisheries due to non-renewal of coastal fish fleets, - The transfer of part of the lobster fishermen's activities to fish, since the lobster and its accompanying catch no longer seemed able to insure the economic viability of new ships. Other maritime districts resolved their lobster problem in a different way. The Lorient fishermen improved their yields by using more capable trawlers, the fishermen of Sable by exploiting the hake fish run to the fullest and limiting their lobster fishing to the best season. The Bigouden region is slowly turning to the second solution, as it renews its fleet.
A ses origines, la flottille côtière du pays bigouden péchant au chalut, est tout entière orientée vers l'exploitation de la langoustine et du merlu. Il s'agit d'une activité traditionnelle qui s' amplifie entre la dernière guerre et les années 70 pour atteindre son apogée entre 1975 et 1980. La meilleure image que l'on puisse donner de cette flottille est celle d'une "noria" débarquant inlassablement sa capture dans les quatre ports du quartier du Guilvinec. Là où existe une activité hauturière à Guilvinec, Loctudy et Saint-Guénolé, il y a une complémentarité qui s'exprime de deux manières : - la pêche côtière constitue un appoint très appréciable et régulier de production, puisque la vente est quotidienne et que seules les intempéries hivernales entraînent une baisse relative des apports. - la pêche côtière donne du port une image flatteuse basée sur la présentation de produits d'appel de grande qualité : langoustine vivante, merlu et baudroies quotidiens. Dans cette étude, nous avons surtout tenté de faire le point sur le système côtier bigouden en tant qu'entité, en englobant dans la même analyse des espèces, les flottilles et l'exploitation de la ressource. Pour les espèces principales, un bilan est proposé. Il couvre séparément la langoustine, le merlu et les baudroies. La langoustine, en raison de ses faibles déplacements, peut être considérée comme constituant un stock isolé, alors que les poissons appartiennent à des ensembles beaucoup plus vastes. La langoustine a beaucoup gagné des diverses augmentations de maillage qui ont été imposées depuis 1971, même si elles n'ont pas toujours été appliquées avec rigueur. Un des résultats de cette gestion par les maillages est un "amortissement" des variations des rendements journaliers autour d'une valeur moyenne de 100 kg. Pour le merlu, le trait dominant est une augmentation des rendements dans les dernières années en accord avec une amélioration de la situation dans le Golfe de Gascogne due à une baisse généralisée de l'effort et à une modification du profil d'exploitation depuis l'apparition des palangriers et des filets droits. Si la situation sur les pêcheries de langoustine, au regard de la taille marchande du merlu, ne s'est pas améliorée puisque la demande de petits merluchons existe toujours, on assiste par contre depuis une dizaine d'années à une diversification lente des activités des flottilles entrant beaucoup mieux dans le schéma logique d'exploitation des pêcheries séquentielles de merlu. Quand les rendements en langoustine baissent, une partie de la flottille s'oriente vers les poisson sur les marges des pêcheries de langoustine. Pour les baudroies on observe des phénomènes assez analogues et une augmentation importante des débarquements des deux espèces par les langoustiniers due également à un élargissement de leurs zones de pêche. Sur les vasières à langoustine elles-mêmes, il n'y a pas de modification notable de l'exploitation dans la série historique. Toute étude des pêches côtières se heurte toujours au problème d'acquisition des données statistiques et le pays bigouden n'échappe pas à la règle. Pour améliorer cette situation, en 1987 et 1988, deux points ont été retenus : - une meilleure connaissance de l'effort et des zones fréquentées, par enquête au moment des débarquements à Guilvinec et à Loctudy, - une description détaillée des apports par espèce, par échantillonnage, à Loctudy, sur les 2/3 des ventes côtières, entre décembre 1987 et novembre 1988. Pour cette raison, l'analyse de flottilles en types est encore sommaire et n'a porté que sur une année. L'apparition des tendances nouvelles vers le poisson ne peut être décrite que qualitativement puisque la série historique n'existe pas. La classification des bateaux a été faite par l'analyse des données fournies par les criées. Ce premier diagnostic ne présente pas toutes les garanties en raison surtout d'une description peu fidèle des apports par les criées, déficience que l'on a essayé de pallier en enquêtant à Loctudy. En conclusion, on assiste dans le pays bigouden comme dans d'autres entités à un rééquilibrage entre les activités traditionnelles et des orientations modernes. La pêche de langoustine, quasi exclusive avant les années 1980, tend à décroître pour deux raisons principales : - une baisse généralisée de l'effort sur les pêcheries de langoustine due au nonrenouvellement des flottilles de pêche côtière, - le report d'une partie de l'effort des langoustiniers vers les pêcheries de poissons, la langoustine et sa capture accessoire ne semblant plus pouvoir assurer la rentabilité économique des navires neufs. D'autres quartiers maritimes ont résolu de façon différente leur problème langoustine. Les Lorientais ont amélioré leurs rendements en adoptant des chaluts plus performants, les Sablais en exploitant à fond la séquence des pêcheries de merlu et en limitant leur intervention sur la langoustine à la saison la plus favorable. Le pays bigouden s'engage lentement dans la seconde solution, au rythme du renouvellement de sa flottille.

Droits : info:eu-repo/semantics/openAccess
http://archimer.ifremer.fr/doc/1989/rapport-2202.pdf
http://archimer.ifremer.fr/doc/00000/2202/

Éditeur(s) : Elsevier
Résumé : Dissolved and particulate Hg fluxes in the Lot-Garonne-Gironde fluvial-estuarine system were obtained from observation of daily discharge and suspended particulate matter (SPM) concentrations. In addition to the measurements of the total dissolved (< 0.45 gm) and particulate Hg (> 0.45 gm), called HgTD and HgTp respectively, the dissolved inorganic Hg species (HgRD) were determined monthly. Geochemical background values for HgTp in sediments and SPM were similar to crustal values and to typical concentrations in SPM of non-contaminated river systems, respectively. The Riou Mort watershed already known as the origin of important historical polymetallic (e.g., Cd, Zn) pollution was identified as an important Hg point source. In the downstream Lot River, Hg concentrations were clearly higher than those in other moderately contaminated systems. The mean relative contribution of HgRD to HgTD in the Lot River and in the Garonne River was close to 25% and 50%, respectively, and showed no correlation with water discharge or SPM concentration. Depending on the origin and nature of SPM, HgTp concentrations were correlated or not with particulate organic C (POC). Maximum HgTp concentrations were measured in samples containing low POC concentrations and were attributed to sediment resuspension. In contrast, high POC concentrations (6-17%) during algal blooms were associated with low/moderate HgTp concentrations (< 0.5 mg kg(-1)) at different sites, suggesting that Hg concentrations in fluvial phytoplankton may be limited by bioavailability of dissolved Hg and/or physiologically controlled Hg accumulation. Mercury was mostly (up to 98%) transported in the particulate phase with estimated annual Hg fluxes at the outlet of the Lot River system ranging from 35 to 530 kg a(-1) for the past decade. The minimum anthropogenic component (58-84% of total Hg fluxes) could not be explained by present Riou Mort point source contributions, suggesting important Hg release from contaminated sediment as a major source and from downstream point sources (e.g., coal-fired power plants and/or metal processing industries). HgTp concentrations and fluxes were strongly related to hydrologic variations and were clearly increased by riverbed dredging during lock construction. Therefore, the estimated Hg stocks in the Lot River sediment (5-13 tons) represent an important potential Hg source for the downstream fluvial-estuarine system.
Applied Geochemistry (0883-2927) (Elsevier), 2006-03 , Vol. 21 , N. 3 , P. 515-527

Droits : 2006 Elsevier Ltd All rights reserved
http://archimer.ifremer.fr/doc/2006/publication-1343.pdf
DOI:10.1016/j.apgeochem.2005.12.004
http://archimer.ifremer.fr/doc/00000/1343/

Éditeur(s) : Elsevier Science Bv
Résumé : The advantages of FLOC over clear water (CW) in rearing juvenile L. vannamei and its effects on Vibrio communities were evaluated. Survival rate in FLOC and clear water were recorded and a probiotic was tested under both conditions. Daily growth rate (DGR) was higher in FLOC (p < 0.05) than in CW. Survival in each system increased significantly when a probiotic was included in the diet (p < 0.05). The Vibrionaceae community from the hepatopancreas (HP) and the culture medium did not differ between the two culture media. Nevertheless, a novel group of Vibrio strains was found to be unique to FLOC. No high level of lesions was observed in shrimp tissues from the FLOC + probiotic treatment; it suggests that the probiotic contributed to homeostasis and prevented outbreak of opportunistic pathogenic species.
Aquaculture (0044-8486) (Elsevier Science Bv), 2014-03 , Vol. 424 , P. 215-219

Droits : 2014 Elsevier B.V. All rights reserved.
http://archimer.ifremer.fr/doc/00173/28379/26694.pdf
DOI:10.1016/j.aquaculture.2014.01.008
http://archimer.ifremer.fr/doc/00173/28379/

Éditeur(s) : Elsevier
Résumé : We studied the effects of a lactic acid bacterium, Pediococcus acidilactici (strain MA 18/5M, CNCM), as a dietary probiotic on growth performance and some nutritional and microbiological aspects of the shrimp Litopenaeus stylirostris. Experimental shrimp culture was carried out over 10 weeks, using floating cages of 14 m(2) each set in earthen ponds, in a New Caledonia farm affected by "Summer syndrome", a septicaemic vibriosis caused by Vibrio nigripulchritudo. The experiment design testing probiotic-coated pellets against control was replicated in two earthen ponds. High mortalities, characteristic of Summer syndrome, were observed during the first 2 weeks of the trial. The probiotic improved production in the treated cages from both ponds with increases in the survival rate (7% and 15% respectively) and final biomass (8% and 12% respectively). No differences were recorded among treatments on growth performances, but significant lower food conversion ratios were obtained with probiotic treatment. After 5 weeks of rearing, the Hepatosomatic Index and the adjusted dry weight of the digestive gland were significantly increased by 10% and by 9% respectively in shrimps treated with probiotic. In the meantime, the specific activities of alpha amylase and trypsin in the digestive gland showed a significant effect of the probiotic treatment with respective increases by 35% and 55%. The rise in total trypsin activity following morning feeding was also enhanced by the probiotic treatment (P < 0.001). The highest concentration of probiotic (between 10(4)-10(5) CFU g(-1) of fresh gut) in the shrimp gut was obtained 2 h after feeding. The concentration remained high for 4 h after feeding before decreasing until the next meal. Total bacterial counts on Marine agar and TCBS in the gut were significantly lowered by the probiotic treatment over the 10 weeks of the experiment. Additionally, during the first 2 weeks, prevalence and load of V nigripulchritudo strains in haemolymph was lower in animals fed with the probiotic diet. This study demonstrated, under pond conditions, that feeding shrimp with live terrestrial lactic acid bacteria can be an effective treatment for improving shrimp culture affected by vibriosis. (c) 2008 Elsevier B.V. All rights reserved.
Aquaculture (0044-8486) (Elsevier), 2008-03 , Vol. 275 , N. 1-4 , P. 182-193

Droits : 2008 Elsevier B.V. All rights reserved.
http://archimer.ifremer.fr/doc/2008/publication-4079.pdf
DOI:10.1016/j.aquaculture.2008.01.011
http://archimer.ifremer.fr/doc/00000/4079/

Éditeur(s) : Springer
Résumé : Batch culture experiments using viral enrichment were conducted to test the response of a coastal bacterial community to autochthonous (i.e., co-existing) or allochthonous riverine viruses. The effects of viral infections on bacterial dynamics and activity were assessed by epifluorescence microscopy and thymidine incorporation, respectively, whereas the effect of viral infection on bacterial community composition was examined by polymerase chain reaction-single strand conformation polymorphism 16S ribosomal RNA fingerprinting. The percentages of high nucleic acid-containing cells, evaluated by flow cytometry, were significantly correlated (r (2) = 0.91, n = 12, p < 0.0001) to bacterial production, making this value a good predictor of active cell dynamics along the study. While confinement and temperature were the two principal experimental factors affecting bacterial community composition and dynamics, respectively, additions of freshwater viruses had significant effects on coastal bacterial communities. Thus, foreign viruses significantly reduced net bacterial population increase as compared to the enrichment treated with inactivated virus. Moreover, freshwater viruses recurrently and specifically affected bacterial community composition, as compared to addition of autochthonous viruses. In most cases, the combined treatment viruses and freshwater dissolved organic matter helped to maintain or even enhance species richness in coastal bacterial communities in agreement to the 'killing the winner' hypothesis. Thus, riverine virus input could potentially influence bacterial community composition of the coastal bay albeit with modest modification of bulk bacterial growth.
Microbial Ecology (0095-3628) (Springer), 2009-02 , Vol. 57 , N. 2 , P. 295-306

Droits : 2009 Springer
http://archimer.ifremer.fr/doc/2009/publication-6636.pdf
DOI:10.1007/s00248-008-9428-1
http://archimer.ifremer.fr/doc/00000/6636/

Éditeur(s) : Amer Geophysical Union
Résumé : Large rivers are key hydrologic components in oceanography, particularly regarding air-sea and land-sea exchanges and biogeochemistry. We enter now in a new era of Sea Surface Salinity (SSS) observing system from Space with the recent launches of the ESA Soil Moisture and Ocean Salinity (SMOS) and the NASA Aquarius/Sac-D missions. With these new sensors, we are now in an excellent position to revisit SSS and ocean color investigations in the tropical northwest Atlantic using multi-year remote sensing time series and concurrent in situ observations. The Amazon is the world's largest river in terms of discharge. In its plume, SSS and upper water column optical properties such as the absorption coefficient of colored detrital matter (acdm) are strongly negatively correlated (<-0.7). Local quasi-linear relationships between SSS and acdm are derived for these plume waters over the period of 2010-2013 using new spaceborne SSS and ocean color measurements. Results allow unprecedented spatial and temporal resolution of this coupling. These relationships are then used to estimate SSS in the Amazon plume based on ocean color satellite data. This new product is validated against SMOS and in situ data and compared with previously developed SSS retrieval models. We demonstrate the potential to estimate tropical Atlantic SSS for the extended period from 1998 to 2010, prior to spaceborne SSS data collection.
Journal Of Geophysical Research-oceans (0148-0227) (Amer Geophysical Union), 2015-05 , Vol. 120 , N. 5 , P. 3177-3192

Droits : 2015. American Geophysical Union. All Rights Reserved.
http://archimer.ifremer.fr/doc/00255/36610/35742.pdf
DOI:10.1002/2014JC010109
http://archimer.ifremer.fr/doc/00255/36610/

Résumé : Ocean Thermal Energy Conversion, OTEC, is a concentrated renewable source of energy available in the one third of the world's ocean lying in the tropics. An OTEC plant is a heat engine fed with warm surface water and cold water pumped up from the deep. The difference of temperature, 20°C, is small, it can be used to produce electricity as well as fresh water. Plants can be installed on land or at sea. The main problem is to build and deploy a large diameter pipe to pump the cold water from 600 to 1 000 m in depth. France, who discovered the idea realised some major demonstrations fifty years ago. More recently US and Japanese firms have spent more than dollars 200 millions to develop prototypes and research in this field. Several major OTEC pilot plants are presently discussed mainly hy US, Japanese and French teams. The main challenge is to prove the potential competitivness of OTEC electricity in the 10 to 100 MWe range, with diesel electricity in the 1990's. Production of OTEC desalination water and marine proteins are also under consideration. The present book give a unique overview of both technical and economical aspects of this promising source of energy. [NOT CONTROLLED OCR]
L'Océan présente un potentiel considérable d'énergie renouvelable. Dans ce domaine, l'énergie thermique des mers (ETM) apparaît très riche de promesses. C'est une idée française: elle est due à d'Arsonval (1881). Les travaux de G. Claude et P. Boucherat (1928-1934), les projets d'Abidjan (1940-1956) et de la Guadeloupe (1958) essaieront de la matérialiser. Depuis les années 60, ce sont surtout les Américains et les Japonais qui développent les recherches. La France a repris ses projets à partir de 1978, de même que certains pays d'Europe, Cet ouvrage expose les caractéristiques de l'ETM et fixe les ordres de grandeur qui permettent de dimensionner les composants d'une centrale. Il rend compte des recherches menées dans différents pays pour maîtriser la technologie ETM :aux États-Unis, au Japon, en Grande-Bretagne, en Suède, en France et, plus récemment, en Inde ou à Taïwan. Il examine les potentialités réelles dans 98 pays et territoires qui ont accès au gisement ETM et dont les 2/3 sont dans la mouvance européenne. L'étude traite enfin du coût de cette énergie dans la perspective d'une compétitivité du KWG/ETM avec le KWG/fioul. L'examen porte ainsi sur d'autres actions possibles: production eau douce, aquaculture, froid. [OCR NON CONTRÔLE]

Droits : info:eu-repo/semantics/openAccess
http://archimer.ifremer.fr/doc/1986/rapport-4310.pdf
http://archimer.ifremer.fr/doc/00000/4310/

Éditeur(s) : Wiley-blackwell Publishing, Inc
Résumé : Vibrio splendidus, strain LGP32, is an oyster pathogen associated with the summer mortalities affecting the production of Crassostrea gigas oysters worldwide. Vibrio splendidus LGP32 was shown to resist to up to 10 mu M Cg-Def defensin and Cg-BPI bactericidal permeability increasing protein, two antimicrobial peptides/proteins (AMPs) involved in C. gigas immunity. The resistance to both oyster Cg-Def and Cg-BPI and standard AMPs (polymyxin B, protegrin, human BPI) was dependent on the ompU gene. Indeed, upon ompU inactivation, minimal bactericidal concentrations decreased by up to fourfold. AMP resistance was restored upon ectopic expression of ompU. The susceptibility of bacterial membranes to AMP-induced damages was independent of the ompU-mediated AMP resistance. Besides its role in AMP resistance, ompU proved to be essential for the adherence of V. splendidus LGP32 to fibronectin. Interestingly, in vivo, ompU was identified as a major determinant of V. spiendidus pathogenicity in oyster experimental infections. Indeed, the V. splendidus-induced oyster mortalities dropped from 56% to 11% upon ompU mutation (Kaplan Meier survival curves, P < 0.01). Moreover, in co-infection assays, the ompU mutant was out competed by the wild-type strain with competitive indexes in the range of 0.1-0.2. From this study, ompU is required for virulence of V. splendidus. Contributing to AMP resistance, conferring adhesive properties to V. splendidus, and being essential for in vivo fitness, the OmpU porin appears as an essential effector of the C. gigas/V. splendidus interaction.
Environmental Microbiology (1462-2912) (Wiley-blackwell Publishing, Inc), 2010-04 , Vol. 12 , N. 4 , P. 951-963

Droits : 2010 Society for Applied Microbiology and Blackwell Publishing Ltd
http://archimer.ifremer.fr/doc/00003/11421/8044.pdf
DOI:10.1111/j.1462-2920.2009.02138.x
http://archimer.ifremer.fr/doc/00003/11421/

Éditeur(s) : Elsevier
Résumé : Phosphorus forms with respect to sediment characteristics, such as grain-size, and major chemical elements, were studied in French coastal marine areas (the Bay of Seine and the Loire and Gironde Estuaries).In the three areas, Fe/Al-bound phosphate (Fe/Al-P) and exchangeable phosphate (exch-P) were significantly related to the proportion of fine fraction (< 63 mum). The special association of these forms with fine particles, as well as the relationships between exch-P, Fe/Al-P and Fe showed that adsorption processes on Fe oxides had taken place. However, for similar Fe concentrations, Bay of Seine sediments showed Fe/Al-P concentrations which were four to five times higher than those found in the Loire and Gironde Estuaries. This was attributed to differences in sediment nature and processes in these two types of areas. Indeed, the Loire and Gironde Estuaries frequently show hypoxia.Calcium-bound phosphate (Ca-P) was found in all grain size classes with comparable concentrations in the Bay of Seine where there was no correlation between Ca-P and Ca. This corresponded to the calcium's marine origin (shells) in the Bay of Seine. In contrast, the correlation of Ca-P with Ca and the fine fraction of the sediment in the Loire and Gironde Estuaries was characteristic of the predominant metamorphic origin of Ca-P in these areas.Useful information for interpretation of P-forms can be obtained from major sediment characteristics. Thus, for areas where specific relationships have previously been established good estimates of P-forms could be predicted from sediment properties.
Estuarine, Coastal and Shelf Science (0272-7714) (Elsevier), 2001-05 , Vol. 52 , N. 5 , P. 617-629

Droits : 2001 Academic Press. All rights reserved
http://archimer.ifremer.fr/doc/2001/publication-469.pdf
DOI:10.1006/ecss.2001.0766
http://archimer.ifremer.fr/doc/00000/469/

Résumé : Depuis maintenant plus de trois ans, l'équipe IFREMER de Martinique travaille à la mise au point technique de l'élevage de l'ombrine subtropicale (Sciaenosp ocellata), dans des conditions intensives. En matière de reproduction, l'équipe IFREMER a engagé dès 1987, une opération de transfert de la technologie Américaine de maturation et ponte grâce au contrôle environnemental de la température et de la phtopériode. Près de 50 millions d'oeufs ont été produits en 1989, à partir de 5 femelles de 5 à 6 kg. En terme d'alevinage, les "résidus d'expérimentation" ont été de 12 000, 18 000, 9 000 et 89 000 alevins sevrés (1 à 2g), respectivement pour les années 1987, 88, 89 et 1990. En 1990, l'objectif principal de démonstration de la faisabilité de la technique d'alevinage en intensif a été atteint avec succès à travers trois élevages "pilotes" successifs aux taux de survie moyen, à 2 grammes, de 14% , 15% et 24%. En expérimentation larvaire, un effort conséquent a été réalisé sur l'approche des besoins nutritionnels de l'espèce. La valeur limite en acides gras essentiels se situerait pour l'ombrine, entre 0,15% et 0,25% de 20:5w3 (en % de poids sec de nauplii) ou entre 0,22% et 0,33% des 2 AGLOPI essentiels: le 20:5w3 et le 22:6w3. En phase de grossissement, les élevages ont permis d'obtenir des poissons de 400 g de poids moyen en 6 à 7 mois en cages flottantes. Le poids initial des alevins est d'environ 2 g pour 2 mois d'âge. L'aliment utilisé est un granulé de type "loup", non spécifique, à 52% de protéines et près de 9% de lipides. Avec cet aliment, les taux de nutrition évoluent de 5, 0-5, 5% en début d'élevage, pour finir à 1,0-1,5% en fin d'élevage (350-500g). Les taux de conversion expérimentaux chez les aquaculteurs, sont compris entre 1,2-2,3. Les charges moyennes des élevages sont de 10 à 15 kg/m3, et la charge maximale enregistrée, de 20-22 kg/m3. La survie moyenne est de 60-70%.

Droits : info:eu-repo/semantics/openAccess
http://archimer.ifremer.fr/doc/00130/24140/22146.pdf
http://archimer.ifremer.fr/doc/00130/24140/

Éditeur(s) : Gauthier-Villars
Résumé : The variability of ''yellow substance'' in waters of the Skagerrak and the Kattegat was studied during the international Skagex programme in 1990 and 1991. Data on attenuation of light and fluorescence of dissolved organic matter (DOM) in sea water were collected with a spectral transmissiometer and a submersible fluorometer within a depth range of 0-250 m at several stations. More than fifty-fold variations of fluorescence intensity over space and time were due to the combined action of different sources of DOM in the straits. The surface maximum of fluorescing DOM, related to river outflow, was spreading from Oslo fjord to the central Skagerrak in late spring. This maximum may be ecologically important because it diminishes shortwave PAR. In the autumn the brightest DOM fluorescence was found in the Kattegat and along the Norwegian coast in accordance with earlier studies. The local minima of DOM fluorescence are associated with the Jutland current. Both the instability of the current and a ten-fold difference in DOM content between deep layers of the Skagerrak and Kattegat resulted in strong synoptic variability of fluorescence in the transitional area between the straits. Seasonal and synoptic variabilities of ''yellow substance'' were minimal in the southern Kattegat. The vertical gradients of DOM fluorescence were mostly negative at the depth of the thermocline of the open Skagerrak in spring, reaching values of 40-50 % m-1. Fine structure and complex shape were inherent to fluorescence profiles from areas of intensive water mixing. DOM fluorescence closely covaried with attenuation of UV light: the correlation coefficient was + 0.9 for wavelength w <= 350 nm regardless of season. The inverse dependence between wavelength derivative and magnitude of UV attenuation in the straits did not change with season. DOM fluorescence and water salinity were negatively correlated at depths of 25-50 m but they varied independently in the upper 10 m. No significant correlation between fluorescence and nitrates has been found. Estimates of correlation with phosphates ranged from 0.7 to 0.8. In general, the changes of fluorescence were due to variations of DOM content in sea water but not to variability of DOM composition or state. The properties of DOM fluorescence in the Skagerrak-Kattegat area make it a useful natural tracer of water movements suitable for active remote sensing with airborne lidar.
Oceanologica Acta (0399-1784) (Gauthier-Villars), 1993 , Vol. 16 , N. 2 , P. 115-125

Droits : info:eu-repo/semantics/openAccess
http://archimer.ifremer.fr/doc/00099/21041/18667.pdf
http://archimer.ifremer.fr/doc/00099/21041/

Éditeur(s) : Elsevier Science Bv
Résumé : A 30-day trial was performed to evaluate the reproductive performance of wild and 2nd generation domesticated Farfantepenaeus duorarum broodstock. Changes in biochemical composition and fatty acids (FA) profile in the 1st and 4th spawn order females were used as indicators of nutritional condition. Wild population of F. duorarum presented significantly better reproductive outcomes as compared to domesticated ones. Wild spawners achieved significantly higher number of eggs per spawn, number of egg per spawn per g of spawner's body weight and number of nauplii per spawn (P<0.05). Latency period was significantly shorter (P<0.05) for wild than for domesticated females (11 vs 17 days). Wild spawners also presented less mortality (6 vs 12%), higher number of total spawns (51 vs 33), spawns per ablated and per spawning females as well as higher total eggs and nauplii production. On the other hand, domesticated females presented higher number of females that spawned at least once (89 vs 76%). The higher spawning activity was reflected in lower levels of acylglycerides (AG) and cholesterol content in wild hepatopancreas (HP) and ovary (OV) (P<0.05). FA profile, mainly represented by ARA, EPA and sum of n-3 were higher in hepatopancreas and ovary of wild females, however lower levels were detected in eggs as a result of a high spawning activity. Multivariate analysis performed to detected simultaneous variation in biochemical variables indicated AG as variation pattern's responsive. (c) 2012 Elsevier B.V. All rights reserved.
Aquaculture (0044-8486) (Elsevier Science Bv), 2012-05 , Vol. 344 , P. 194-204

Droits : 2012 Elsevier B.V. All rights reserved.
http://archimer.ifremer.fr/doc/00079/19042/17214.pdf
DOI:10.1016/j.aquaculture.2012.03.014
http://archimer.ifremer.fr/doc/00079/19042/

Éditeur(s) : Gauthier-Villars
Résumé : An experimental techniques to estimate the influence of dissolved organic matter (DOM) on remote sensing of chlorophyll in water has been developed, based on the relationship between fluorescence and absorption of light by a substance. The profiles of fluorescence, chlorophyll content in sea water and estimates of light attenuation in water samples were used in the paper. These data have permitted to calculate the regressions linking fluorescence and light absorption at 450 nm by chlorophyll (ABC450) and by DOM (ABD450). The remote sensing needs no correction for DOM if M450 = 100* (ABC450/ABD450) much greater than 100. This technique has been applied to data collected during the international Skagex field experiment in the straits of Skagerrak and Kattegat in spring and autumn of 1990. It was found that M450 <= 100 everywhere in the subsurface layer. Unfavorable conditions for remote sensing of chlorophyll prevailed in the straits because of the uptake of DOM-rich water from the continent and the accumulation of phytoplankton in deeper layers. These and another processes can increase relative content of colored DOM even in waters of open ocean.
Oceanologica Acta (0399-1784) (Gauthier-Villars), 1992 , Vol. 15 , N. 3 , P. 255-259

Droits : info:eu-repo/semantics/openAccess
http://archimer.ifremer.fr/doc/00100/21159/18778.pdf
http://archimer.ifremer.fr/doc/00100/21159/

Éditeur(s) : Inter-Research
Résumé : The pathogenicity of Vibrio penaeicida Strains KH-1 and AM101, their culture-free supernatant (CFS), and their protein fraction obtained by 40% of ammonium sulfate precipitation (PFs40) were assessed in experimental challenges against juvenile Litopenaeus vannamei. Live Vibrio cells, CFS, and PFs40 from the AM101 strain produced a significantly higher mortality (p < 0.05) compared to the KH-1 strain. Toxicity and median lethal doses (LD50) of Fast Protein Liquid Chromatography (FPLC) products were evaluated on L. vannamei. The first FPLC fraction sample (A) from PFs40 of the AM101 strain displayed LD50 values of 1.68 and 5.61 mu g protein ind.(-1), respectively. The second FPLC process from Fraction A showed a peak (A1) also with toxic effects to shrimp. PFs40, Fraction A, and Peak At showed a 38.5 kDa molecular band (SDS-PAGE), with activity on a gelatin protease zymogram. The lethal effect of PFs40 and Fraction A was inhibited by Proteinase K, CuCl2, E-64, and heat (60 and 100 degrees C) treatments, but was not inhibited by EDTA-Na-2, aprotinin, and soy trypsin treatments. These results and the zymogram inhibition test suggest the presence of a cysteine protease-like proteinaceous exotoxin as a dominant protease, secreted by V. penaeicida Strain AM101.
Diseases of aquatic organisms (0177-5103) (Inter-Research), 2005-11 , Vol. 67 , N. 3 , P. 201-207

Droits : Inter-Research 2005
http://archimer.ifremer.fr/doc/2005/publication-3618.pdf
DOI:10.3354/dao067201
http://archimer.ifremer.fr/doc/00000/3618/

Éditeur(s) : Elsevier
Résumé : Red drum larvae were fed from day 11 to day 24 post-hatch, with Artemia (LP group) or two isoproteinic-formulated diets with varying lipid levels, 15% (L15 group) and 30% (L30 group). The lipid fraction was composed of a mixture of cod liver oil and soy lecithin. Larvae fed Artemia exhibited the greatest length, L30 diet induced a larvae length significantly greater than that obtained with L15 diet (P < 0,05). No difference was observed in larvae survival among the three dietary groups. The increase in dietary lipid concentration led to an increase in amylase and trypsin secretion levels in 24-day-old larvae. The pancreatic secretion level measured in L30 group was close to that of LP group, suggesting that the maturation of the pancreatic functions was normally developed. Red drum larvae fed the diet containing the higher lipid content (L30) exhibited a more developed brush border membrane digestion in the intestine than larvae fed L15 diet (P < 0.05). Phospholipase A2 was stimulated by the increase of phospholipids in the diet, but no difference was observed for lipase between the two compound diet-fed groups; the LP group exhibited the lowest lipase activity. A plateau in lipase activity may have occurred in red drum larvae from 15% fat level in the diet, suggesting that the maximal lipase activity was reached with 12% neutral lipid in the diet. The positive effects observed with high dietary fat may be due to the phospholipid fraction of the lipid mixture used.
Aquaculture (0044-8486) (Elsevier), 2000-04 , Vol. 184 , N. 3-4 , P. 339-347

Droits : 2000 Elsevier
http://archimer.ifremer.fr/doc/2000/publication-503.pdf
DOI:10.1016/S0044-8486(99)00325-7
http://archimer.ifremer.fr/doc/00000/503/

Éditeur(s) : Springer
Résumé : The distribution of summer phytoplankton across the Straits of Magellan (SOM) was studied with the aims of tracing differences among the distinct subregions of the area and contributing to the knowledge of its biodiversity. Samples collected at 25 stations were observed and counted in light microscopy. Selected samples were observed with transmission electron microscopy. The main unifying feature of the phytoplankton in the SOM was the high abundance and numerical dominance of small-sized (<10 μm) eukaryotic species, among which coccoid cells of <3 μm size were predominant (56.2 ± 30.6 of the total phytoplankton abundance). They mostly belonged to the prasinophyte Pycnococcus provasolii, which was abundant (0.8–6,834 cells × 103 ml−1) at all stations with the exception of those in proximity to the Atlantic entrances, where it was not recorded. Small-sized (<3 and 3–5 μm) diatoms (Minidiscus trioculatus, Lennoxia faveolata and other undetermined centric species) attained high densities (<3,757 cells 103 ml−1) especially at stations of the Patagonian sectors, whereas microplanktonic diatoms were only found at the two entrances of the Straits. Dinoflagellates were constituted mainly by >10 μm forms in the Andean subregion and <10 μm naked species in the Patagonian subregion, contributing up to 75.9 and 41.8% of the total carbon in these two areas, respectively. In the Patagonian subregion, flagellates mainly constituted by <5 μm forms and by cryptomonads <10 μm comprised up to 53.9% of the total biomass. Several species identified in this study have never been reported in other investigations in the SOM, while others, including Pycnococcus provasolii and Lennoxia faveolata, have rarely been recorded elsewhere. Overall, the summer phytoplankton of the Straits does not resemble that of any other region of the world’s seas. Although some of the predominant species might have been overlooked elsewhere, their abundance and relative importance apparently constitute a distinctive feature of the SOM.
Polar Biology (0722-4060) (Springer), 2011 , Vol. 34 , N. 9 , P. 1269-1284

Droits : The Author(s) 2010. This article is published with open access at Springerlink.com
http://archimer.ifremer.fr/doc/00026/13722/10828.pdf
DOI:10.1007/s00300-010-0937-2
http://archimer.ifremer.fr/doc/00026/13722/

Éditeur(s) : Wiley-blackwell
Résumé : The reef fauna connectivity of the West Indian Ocean (WIO) is one of the least studied globally. Here we use genetic analyses of the grouper Epinephelus merra (Bloch 1793) to determine patterns of connectivity and to identify barriers to dispersal in this WIO marine area. Phylogeographic and population-level analyses were conducted on cytochrome b sequences and microsatellites (13 loci) from 557 individuals sampled in 15 localities distributed across the West Indian Ocean. Additional samples from the Pacific Ocean were used to benchmark the WIO population structure. The high level of divergence revealed between Indian and Pacific localities (of about 4.5% in sequences) might be the signature of the major tectonic and climatic changes operating at the Plio-Pleistocene transition, congruently with numerous examples of Indo-Pacific speciation. In comparison, the E. merra sequences from the Indian Ocean constitute a monophyletic clade with a low average genetic distance (d < 0.5%). However both genetic markers indicated some structure within this ocean. The main structure revealed was the isolation of the Maldives from the WIO localities (a different group signature identified by clustering analysis, great values of differentiation). Both marker types reveal further significant structure within the WIO, mainly the isolation of the Mascarene Islands (significant AMOVA and isolation-by-distance patterns) and some patchy structure between the northernmost localities and within the Mozambique Channel. The WIO genetic structure of E. merra appeared congruent with main biogeographic boundaries and oceanographic currents.
Marine Ecology-an Evolutionary Perspective (0173-9565) (Wiley-blackwell), 2015-09 , Vol. 36 , N. 3 , P. 447-461

Droits : 2014 Blackwell Verlag GmbH
http://archimer.ifremer.fr/doc/00189/30046/28566.pdf
DOI:10.1111/maec.12153
http://archimer.ifremer.fr/doc/00189/30046/