Éditeur(s) : HAL CCSD Public Library of Science
Résumé : International audience
BACKGROUND: The use of pesticides and the related environmental contaminations can lead to human exposure to various molecules. In early-life, such exposures could be responsible for adverse developmental effects. However, human health risks associated with exposure to complex mixtures are currently under-explored. OBJECTIVE: THIS PROJECT AIMS AT ANSWERING THE FOLLOWING QUESTIONS: What is the influence of exposures to multiple pesticides on the metabolome? What mechanistic pathways could be involved in the metabolic changes observed? METHODS: Based on the PELAGIE cohort (Brittany, France), 83 pregnant women who provided a urine sample in early pregnancy, were classified in 3 groups according to the surface of land dedicated to agricultural cereal activities in their town of residence. Nuclear magnetic resonance-based metabolomics analyses were performed on urine samples. Partial Least Squares Regression-Discriminant Analysis (PLS-DA) and polytomous regressions were used to separate the urinary metabolic profiles from the 3 exposure groups after adjusting for potential confounders. RESULTS: The 3 groups of exposure were correctly separated with a PLS-DA model after implementing an orthogonal signal correction with pareto standardizations (R2 = 90.7% and Q2 = 0.53). After adjusting for maternal age, parity, body mass index and smoking habits, the most statistically significant changes were observed for glycine, threonine, lactate and glycerophosphocholine (upward trend), and for citrate (downward trend). CONCLUSION: This work suggests that an exposure to complex pesticide mixtures induces modifications of metabolic fingerprints. It can be hypothesized from identified discriminating metabolites that the pesticide mixtures could increase oxidative stress and disturb energy metabolism.
ISSN: 1932-6203

inserm-00863407
http://www.hal.inserm.fr/inserm-00863407
http://www.hal.inserm.fr/inserm-00863407/document
http://www.hal.inserm.fr/inserm-00863407/file/journal.pone.0064433.pdf
PUBMED : 23704985

Éditeur(s) : HAL CCSD Nature Publishing Group
Résumé : International audience
Polarization dependence second harmonic generation (P-SHG) microscopy is gaining increase popularity for in situ quantification of fibrillar protein architectures. In this report, we combine P-SHG microscopy, new linear least square (LLS) fitting and modeling to determine and convert the complex second-order non-linear optical anisotropy parameter ρ of several collagen rich tissues into a simple geometric organization of collagen fibrils. Modeling integrates a priori knowledge of polyhelical organization of collagen molecule polymers forming fibrils and bundles of fibrils as well as Poisson photonic shot noise of the detection system. The results, which accurately predict the known sub-microscopic hierarchical organization of collagen fibrils in several tissues, suggest that they can be subdivided into three classes according to their microscopic and macroscopic hierarchical organization of collagen fibrils. They also show, for the first time to our knowledge, intrahepatic spatial discrimination between genuine fibrotic and non-fibrotic vessels. CCl 4-treated livers are characterized by an increase in the percentage of fibrotic vessels and their remodeling involves peri-portal compaction and alignment of collagen fibrils that should contribute to portal hypertension. This integrated P-SHG image analysis method is a powerful tool that should open new avenue for the determination of pathophysiological and chemo-mechanical cues impacting collagen fibrils organization.
ISSN: 2045-2322

hal-01592192
https://hal.archives-ouvertes.fr/hal-01592192
https://hal.archives-ouvertes.fr/hal-01592192/document
https://hal.archives-ouvertes.fr/hal-01592192/file/s41598-017-12398-0.pdf
DOI : 10.1038/s41598-017-12398-0

Éditeur(s) : HAL CCSD Elsevier
Résumé : International audience
Deep crustal and mantle rocks are exhumed in core complex mode of extension in three types of structures: metamorphic core complexes, oceanic core complexes and magma poor passive margins. Using available analogue and numerical models and their comparison with natural examples, the present paper reviews the mechanical processes involved in these different types of extensional setting. Three main aspects are considered: i) the primary role of lithosphere rheology, ii) the lithosphere-scale patterns of progressive deformation that lead to the exhumation of deep metamorphic or mantle rocks and iii) the initiation and development of detachment zones. Crustal core complexes develop in continental lithospheres whose Moho temperature is higher than 750 °C with “upper crust-dominated” strength profiles. Contrary to what is commonly believed, it is argued from analogue and numerical models that detachments that accommodate exhumation of core complexes do not initiate at the onset of extension but in the course of progressive extension when the exhuming ductile crust reaches the surface. In models, convex upward detachments result from a rolling hinge process. Mantle core complexes develop in either the oceanic lithosphere, at slow and ultra-slow spreading ridges, or in continental lithospheres, whose initial Moho temperature is lower than 750 °C, with “sub-Moho mantle-dominated” strength profiles. It is argued that the mechanism of mantle exhumation at passive margins is a nearly symmetrical necking process at lithosphere scale without major and permanent detachment, except if strong strain localization could occur in the lithosphere mantle. Distributed crustal extension, by upper crust faulting above a décollement along the ductile crust increases toward the rift axis up to crustal breakup. Mantle rocks exhume in the zone of crustal breakup accommodated by conjugate mantle shear zones that migrate with the rift axis, during increasing extension.
ISSN: 0040-1951

insu-01610186
https://hal-insu.archives-ouvertes.fr/insu-01610186
https://hal-insu.archives-ouvertes.fr/insu-01610186/document
https://hal-insu.archives-ouvertes.fr/insu-01610186/file/brun-tect-2017.pdf
DOI : 10.1016/j.tecto.2017.09.017

Éditeur(s) : HAL CCSD American Geophysical Union
Résumé : International audience
We propose in this paper an original method for quantifying the material losses at subduction zones mainly based on vertical movements of the active margins. We first test the method, using isostatic and elastic models, applying it to 15 different well-constrained convergent margins. The main conclusion is that most of them are presently close to the isostatic equilibrium. Consequently, we consider as a first hypothesis that such was also the case in the past and that erosion or accretion are natural mechanisms restoring the equilibrium in response to a perturbation. Then, we apply this model to compute the geometry of the palcoslab knowing the palcotopography of the margin. The comparison between present and past ocean margin lithospheric wedge geometries provides estimates of the removed material that constituted the initial wedge. Two cases were studied for palcoreconstructions: Japan and Peru. The results show that a considerable amount of material was removed during the Neogene along both margins. A detailed interpretation of the Neogene history of the Japan margin is proposed on the basis of our results.
ISSN: 0148-0227

hal-01261647
https://hal.archives-ouvertes.fr/hal-01261647
https://hal.archives-ouvertes.fr/hal-01261647/document
https://hal.archives-ouvertes.fr/hal-01261647/file/lallemanJGR1992.pdf
DOI : 10.1029/91JB02342

Éditeur(s) : HAL CCSD
Résumé : International audience
The Aegean domain is a key area for understanding the processes of back-arc extension. Observed deformation pattern and present day kinematics result from the interaction between the southward retreat of the Hellenic trench and the westward escape of Anatolia. Using laboratory experiments designed to study lithosphere-scale deformation, we show that the overall pattern of Aegean extension requires not only the combination of trench retreat and Anatolia escape since 15 Ma but also the presence of an inherited lithosphere-scale mechanical discontinuity: the Vardar Suture Zone (VSZ). The reactivation in dextral shear of the eastern branch of the VSZ accommodates both the trench retreat (NS stretching) and the westward escape of Anatolia (EW shortening) in the Cyclades area since 15 Ma. Additionally, our model shows that the North Anatolian Fault (NAF) is a late structure in the evolution of the Aegean, initiated 10 Ma after the onset of Anatolia escape. The model displays displacement sub-domains, which result from strain partitioning instead of being “rigid microplates”, directly comparable to the present-day displacement field (GPS) of the Aegean and western Anatolia. Our modelling provides a simple way to look at the dynamics of Aegean extension in two main stages. From middle Eocene to middle Miocene, extension was only driven by the southward retreat of the Hellenic trench at a rate lower than 1 cm.y-1. Since middle Miocene, the combination of slab rollback with Anatolia westward escape, resulted in a southwest direction of trench retreat, with an accelerating rate of up to 3 cm.y-1.
AGU Fall Meeting 2015
San Francisco, United States

insu-01242045
https://hal-insu.archives-ouvertes.fr/insu-01242045

Éditeur(s) : HAL CCSD Oxford University Press (OUP)
Résumé : International audience
Anisotropy of low-field magnetic susceptibility (AMS) of basaltic lava flows can give some clues about post-emplacement tilting occurring in volcanic sequences. Such a study has been carried out on a sequence of 19 successive lava flows from Kerguelen Archipelago. Surprisingly, two different patterns were observed. The first one - herein called normal fabric - is consistent with the lava-flow direction inferred from geological observations, whereas the second one observed for about 70 per cent of the analysed samples - herein called abnormal fabric - appears to be unrelated to the shear history of the lava flows during their emplacements. These abnormal fabrics are not strictly inverse fabric but seem to have a more complex origin. The unusual nature of these abnormal fabrics has been confirmed by lattice preferred orientation of plagioclases obtained from high-resolution electron backscattered diffraction measurements. The aim of this study is to propose a physical interpretation of these fabrics. A first step in our understanding was to clearly identify the nature and the magnetic properties of the Fe-Ti oxides assemblage. Thus, we present a comprehensive rock magnetic analysis relying on low- and high-temperature thermomagnetic curves (K-T), crossover diagrams, first-order reversal curves, ore-microscopy, Mössbauer measurements and electron backscattered images. We found that in the present case the abnormal fabrics are probably linked to a mixture of prevailing single domain (SD) strongly interacting grains population and a subsidiary multidomain grains population. SD grains are identified as small magnetite crystallized within the interstitial glass in globular aggregates along the silicate framework. Then a question arises: to what extent such SD interacting grains in globular aggregates can affect the magnetic susceptibility and its anisotropy? To answer this question, we developed a physical model in which the total energy density of an SD grain with an uniaxial anisotropy is minimized. In this model, the distribution function of the direction of the magnetic interaction field is comprised between two boundary states. It is either isotropic, which formally corresponds to the thermal demagnetized state, or it is ordered, which formally corresponds to the Alternative Field demagnetized state. We show that in both cases the degree of anisotropy decreases as the interaction field increases. Thus, we conclude that the abnormal fabric encountered in this study can be simply explained by strong magnetostatic interactions that would explain the large scattering of AMS often observed in basaltic lava flows.
ISSN: 0956-540X

hal-00716449
https://hal.archives-ouvertes.fr/hal-00716449
https://hal.archives-ouvertes.fr/hal-00716449/document
https://hal.archives-ouvertes.fr/hal-00716449/file/gji-S-12-HAL.pdf
DOI : 10.1111/J.1365-246X.2012.05421.X

Éditeur(s) : HAL CCSD Institute for Scientific Computing and Information
Résumé : International audience
The production of oil in soft highly compacted reservoirs produces a significant reduction in the pore space which in turn causes the surface to subside. The authors couple two simulators to model the mechanical deformations in the presence of a Darcy flow, both one- and two-phase. The simulators solve for flow in a porous medium using a finite volume method, and solve for the mechanical deformation of the porous media using a finite element method. The flow equations and the deformation equations are coupled using Biot's law. The authors use two different methods for the coupling: the first uses a staggered algorithm; the second is based on a preconditioned conjugate method. The authors use a multiscale method in both approaches. The authors show that the inf-sup condition is not satisfied, because of the mixed discretization which is done by using two separate simulators. The authors give convergence comparisons for the two methods for several examples. Two of the examples are for the linear problem (one-phase) where they know the exact solution and one is for the nonlinear problem (two-phase). In all cases, the preconditioned conjugate algorithm is faster and more robust, in particular when the inf-sup condition is not satisfied. In the nonlinear problem the authors state that the results agree with physical predictions but do not give actual empirical data. Finally, they show the effects of grid orientation on the nonlinear problem.
ISSN: 1705-5105

hal-00730673
https://hal.archives-ouvertes.fr/hal-00730673

Éditeur(s) : HAL CCSD AGU and the Geochemical Society
Résumé : International audience
The 2004 Aceh and 2005 Nias events are the two greatest earthquakes of the past 40 years with a total rupture of 1700 km long and a coseismic slip reaching up to 25 m. These two earthquakes have caused large stress perturbations which significantly altered seismic activity in the Sumatra-Andaman region. Using both detailed mapping of failure planes and various slip distributions, we calculate this stress change along the Sumatra-Andaman-Sagaing fault system from central Sumatra to southern Myanmar. The static Coulomb stress change Delta CFF and the observed seismic activity are in very good agreement with a Coulomb index similar to 20% greater than the one obtained for random events. Compared to previous studies, this high Coulomb Index confirms two important issues on the use of static stress change criterion: unsuited to study near-field aftershocks and only relevant for aftershocks analysis on large and mature faults at a time scale of several months. The calculated Delta CFF distribution suggests that the 2004 and 2005 earthquakes inhibit failure on the North Andaman rift and on the Sagaing fault, while failure is encouraged along the transform Andaman zone, the central Andaman rift, the West Andaman fault, the Sumatra fault system, and the offshore thrust faults west of Sumatra Island. The maximum value of similar to 15-20 bar (1.5-2 MPa) for Delta CFF is reached in the northern part of the Sumatra fault system. This high value together with the lack of major earthquake in the last 170 years result in a high seismic hazard for this region. Our results are also consistent with temporal evolution of both earthquakes' location and focal mechanism prior to and after the events. In particular, we explain the occurrence and the mechanism of seismic swarms observed in the central Andaman rift and along the west Andaman fault. Finally, our calculations reveal that the seismicity in the Andaman rift zone can only be explained if mu' > 0.5. This result leads to two end-member models: one with a constant and high fault friction and one with spatial variations, for which friction may depend on either the nature of the lithosphere (oceanic versus continental) or the fault type.
ISSN: 1525-2027

hal-00456027
https://hal.archives-ouvertes.fr/hal-00456027
DOI : 10.1029/2008GC002167

Éditeur(s) : HAL CCSD De Gruyter
Résumé : International audience
A comparative study of 9H-pyrido[3,4-b]indole (norharmane) and 1-methyl-9H-pyrido[3,4-b]indole (harmane) as inhibitors for C38 steel corrosion in 1 M HCl solution at 25 °C was carried out. Potentiodynamic polarization and electrochemical impedance spectroscopy (EIS) techniques were applied to study the metal corrosion behavior in the absence and presence of different concentrations of these inhibitors. The OCP as a function of time were also established. Cathodic and anodic polarization curves show that norharmane and harmane are a mixed-type inhibitors. Adsorption of indole derivatives on the C38 steel surface, in 1 M HCl solution, follows the Langmuir adsorption isotherm model. The ΔGads values were calculated and discussed. The potential of zero charge (PZC) of the C38 steel in inhibited solution was studied by the EIS method, and a mechanism for the adsorption process was proposed. Raman spectroscopy confirmed that indole molecules strongly adsorbed onto the steel surface. The electronic properties of indole derivates, obtained using the AM1 semi-empirical quantum chemical approach, were correlated with their experimental efficiencies using the linear resistance model (LR).
ISSN: 0018-3830

hal-01093227
https://hal-agroparistech.archives-ouvertes.fr/hal-01093227
DOI : 10.1515/HF.2010.034

Éditeur(s) : HAL CCSD Public Library of Science
Résumé : International audience
The extinct Odontopterygiformes are the sole birds known to possess strong and sharp bony pseudoteeth, the shape and location of which are closely mimetic of real teeth. The structure of the pseudoteeth is investigated here in a late Pliocene/early Pleistocene species, Pelagornis mauretanicus, using X-ray microtomography and thin sections. The results are interpreted with regard to the pseudotooth mode of growth, and have implications concerning aspects of Pelagornis ecology. The larger pseudoteeth are hollow and approximately cone-shaped, and the smaller ones are rostro-caudally constricted. The walls of pseudoteeth are composed of bone tissue of the fibro-lamellar type, which is intensively remodeled by Haversian substitution. The jaw bones display the same structure as the pseudoteeth, but their vascular canals are oriented parallel to the long axis of the bones, whereas they are perpendicular to this direction in the pseudoteeth. There is no hiatus or evidence of a fusion between the pseudoteeth and the jaw bones. Two possible models for pseudotooth growth are derived from the histological data. The most plausible model is that pseudotooth growth began after the completion of jaw bone growth, as a simple local protraction of periosteal osteogenic activity. Pseudotooth development thus occurred relatively late during ontogeny. The highly vascularized structure and the relative abundance of parallel-fibered bone tissue in the pseudoteeth suggest poor mechanical capabilities. The pseudoteeth were most likely covered and protected by the hardened, keratinized rhamphotheca in the adult during life. The late development of the pseudoteeth would involve a similarly late and/or partial hardening of the rhamphotheca, as displayed by extant Anseriformes, Apterygiformes and some Charadriiformes. This would add support to the hypothesis of a close phylogenetic relationship between Odontopterygiformes and Anseriformes. The late maturation of the Pelagornis feeding apparatus, and hence the delayed capability for efficient prey catching, suggests that Pelagornis was altricial.
ISSN: 1932-6203

hal-01545486
https://hal.archives-ouvertes.fr/hal-01545486
DOI : 10.1371/journal.pone.0080372

Éditeur(s) : HAL CCSD Public Library of Science
Résumé : International audience
Brain-derived neurotrophic factor (BDNF), a member of the neurotrophin family, has emerged as an active mediator in many essential functions in the central nervous system of mammals. BDNF plays significant roles in neurogenesis, neuronal maturation and/or synaptic plasticity and is involved in cognitive functions such as learning and memory. Despite the vast literature present in mammals, studies devoted to BDNF in the brain of other animal models are scarse. Zebrafish is a teleost fish widely known for developmental genetic studies and is emerging as model for translational neuroscience research. In addition, its brain shows many sites of adult neurogenesis allowing higher regenerative properties after traumatic injuries. To add further knowledge on neurotrophic factors in vertebrate brain models, we decided to determine the distribution of bdnf mRNAs in the larval and adult zebrafish brain and to characterize the phenotype of cells expressing bdnf mRNAs by means of double staining studies. Our results showed that bdnf mRNAs were widely expressed in the brain of 7 days old larvae and throughout the whole brain of mature female and male zebrafish. In adults, bdnf mRNAs were mainly observed in the dorsal telencephalon, preoptic area, dorsal thalamus, posterior tuberculum, hypothalamus, synencephalon, optic tectum and medulla oblongata. By combining immunohistochemistry with in situ hybridization, we showed that bdnf mRNAs were never expressed by radial glial cells or proliferating cells. By contrast, bdnf transcripts were expressed in cells with neuronal phenotype in all brain regions investigated. Our results provide the first demonstration that the brain of zebrafish expresses bdnf mRNAs in neurons and open new fields of research on the role of the BDNF factor in brain mechanisms in normal and brain repairs situations.
ISSN: 1932-6203

hal-01359562
https://hal-univ-rennes1.archives-ouvertes.fr/hal-01359562
DOI : 10.1371/journal.pone.0158057
PUBMED : 27336917

Éditeur(s) : HAL CCSD Wiley
Résumé : Sodium, an element which is needed by animals but often toxic in high concentrations to plants, may be deficient and limit animal abundance in inland continental regions, but may be overabundant and limit plant productivity in coastal regions. Here we present data from 50 independent plots (including leaf data from more than 2480 individual trees) showing that leaves in the Amazon basin uptake high amounts of sodium (Na) in a manner more similar to the essential cation potassium (K) than to the toxic cation aluminium (Al). Leaf Na increases linearly with soil Na concentrations, and there is no apparent mechanism for selective exclusion of Na in comparison to K, a key attribute of halophytes. This indicates that the Amazon basin is broadly non-halophytic and increased sodium concentrations in non-halophyte plants often decrease plant productivity. Total Na concentrations are approximate to 10 times higher in coastal regions than inland regions. Such concentration gradients in nutrients may have been reduced in the past because large animals that were abundant in the Pleistocene have been hypothesized to play a large role in reducing nutrient concentration gradients at continental scales. We use a diffusion model and a Na loss rate based on empirical data to estimate that large animals may have moved significant quantities of Na inland away from coastal regions in the Amazon Basin. Therefore, our simple model suggests that large animals may play an important, yet diminishing, role in maintaining the sodium balance of the planet.
ISSN: 0906-7590

hal-01531615
https://hal.archives-ouvertes.fr/hal-01531615
PRODINRA : 349612
DOI : 10.1111/ecog.01589

Éditeur(s) : Elsevier Science Bv
Résumé : Shrimp aquaculture in New Caledonia is subject to seasonal mortalities during grow-out due to highly virulent Vibrio nigripulchritudo (Vn). To understand the mechanisms affecting shrimp resistance and leading to significant mortality, a shrimp ecophysiology and immunology survey was conducted on two farms, the first considered as a "control" farm (HC), the second affected by the disease (DF). Mortality observed during the survey at DF started 50 days after stocking and was typical of this disease. The main observations regarding shrimp were: (a) growth was not affected by the disease and was faster in the DF than in the HC pond; (b) disease did not affect one sex more than the other, or a specific part of the population in terms of weight; (c) the physical condition of shrimp did not specifically allow us to foresee disease outbreak; (d) shrimp at late premolt stage D-2 and early postmolt stage A appeared to be at some points of the mortalities - but not continuously - the most sensitive to disease; (e) physiological, immunological and nutritional parameters of uninfected shrimp in the DF pond were altered, suggesting that environmental stress occurred just before the first mortalities; (f) data suggest that Vn-infected shrimp are more stressed than the presumed healthy shrimp. Combined with pathological and environmental knowledge gained in parallel during this survey, a conceptual model is proposed. Results suggest that an unstable environment induced conditions (i) stressful for the shrimp, increasing their susceptibility to bacterial infections and (ii) favoring the proliferation of the pathogen in the pond. The combination of these two processes could lead to significant mortality.
Aquaculture (0044-8486) (Elsevier Science Bv), 2013-04 , Vol. 388 , P. 105-114

Droits : 2013 Elsevier B.V. All rights reserved.
http://archimer.ifremer.fr/doc/00176/28764/27567.pdf
DOI:10.1016/j.aquaculture.2013.01.010
http://archimer.ifremer.fr/doc/00176/28764/

Éditeur(s) : HAL CCSD Elsevier
Résumé : International audience
Tectonically controlled landforms develop morphologic features that provide useful markers to investigate crustal deformation and relief growth dynamics. In this paper, we present results of morphotectonic experiments obtained with an innovative approach combining tectonic and surface processes (erosion, transport, and sedimentation), coupled with accurate model monitoring techniques. This approach allows for a qualitative and quantitative analysis of landscape evolution in response to active deformation in the three end-member geological settings: compression, extension, and strike–slip.Experimental results outline first that experimental morphologies evolve significantly at a short time scale. Numerous morphologic markers form continuously, but their lifetime is generally short because erosion and sedimentation processes tend to destroy or bury them. For the compressional setting, the formation of terraces above an active thrust appears mainly controlled by narrowing and incision of the main channel through the uplifting hanging-wall and by avulsion of deposits on fan-like bodies. Terrace formation is irregular even under steady tectonic rates and erosional conditions. Terrace deformation analysis allows retrieving the growth history of the structure and the fault slip rate evolution. For the extensional setting, the dynamics of hanging-wall sedimentary filling appears to control the position of the base level, which in turn controls footwall erosion. Two phases of relief evolution can be evidenced: the first is a phase of relief growth, and the second is a phase of upstream propagation of topographic equilibrium that is reached first in the sedimentary basin. During the phase of relief growth, the formation of triangular facets occurs by degradation of the fault scarp, and their geometry (height) becomes stationary during the phase of upstream propagation of the topographic equilibrium. For the strike–slip setting, the complex morphology of the wrench zone, composed of several interacting fault segments, enhances the interactions with the drainage network. Because of the widening of the main fault zone toward the surface, a significant amount of distributed deformation is observed along the wrench zone. Locally, where two terminations of fault segments interact, less than a quarter of the far field displacement can remain measurable using fault offsets, leading to a systematic underestimation of the real fault slip rate.These different experimental examples illustrate the great potential of the approach coupling deformation mechanisms and erosion–transport–sedimentation processes to investigate qualitatively and quantitatively the morphotectonic evolution of tectonically controlled landscapes.
ISSN: 0169-555X

hal-01243587
https://hal.archives-ouvertes.fr/hal-01243587
DOI : 10.1016/j.geomorph.2015.02.011

Éditeur(s) : HAL CCSD Wiley-VCH Verlag
Résumé : International audience
Proteomic profiling plays a decisive role in the elucidation of molecular signatures representative of a specific clinical context. MuStem cell-based therapy represents a promising approach for clinical applications to cure Duchenne Muscular Dystrophy (DMD). To expand our previous studies collected in the clinically relevant DMD animal model, we decided to investigate the skeletal muscle proteome four months after systemic delivery of allogenic MuStem cells. Quantitative proteomics with isotope-coded protein labelling (ICPL) was used to compile quantitative changes in the protein expression profiles of muscle in transplanted Golden Retriever Muscular Dystrophy (GRMD) dogs as compared to GRMD dogs. A total of 492 proteins were quantified, including 25 that were overrepresented and 46 that were underrepresented after MuStem cell transplantation. Interestingly, this study demonstrates that somatic stem cell therapy impacts on the structural integrity of the muscle fascicle by acting on fibres and its connections with the extracellular matrix. We also show that cell infusion promotes protective mechanisms against oxidative stress and favours the initial phase of muscle repair. This study allows us to identify putative candidates for tissue markers that might be of great value in objectively exploring the clinical benefits resulting from our cell-based therapy for DMD.
ISSN: 1615-9853

Droits : info:eu-repo/semantics/OpenAccess
hal-01332030
https://hal.archives-ouvertes.fr/hal-01332030
https://hal.archives-ouvertes.fr/hal-01332030/document
https://hal.archives-ouvertes.fr/hal-01332030/file/Quantitative%20proteome%20profiling%20of%20dystrophic%20dog%20skeletal%20muscle_accepted.pdf
DOI : 10.1002/pmic.201600002

Éditeur(s) : HAL CCSD IODP
Résumé : Integrated Ocean Drilling Program (IODP) Expedition 335 (14 April–4 June 2011) will be the fourth ocean cruise of the "Superfast" campaign to drill a deep hole into intact oceanic basement and will return to Ocean Drilling Program (ODP) Hole 1256D to deepen this scientific reference penetration a significant distance into cumulate gabbros. Cores and data recovered during the Superfast 4 expedition will provide hitherto unavailable observations that will test models of the accretion and evolution of the oceanic crust. Site 1256 was specifically located on oceanic crust that formed at a superfast spreading rate (>200 mm/y) to exploit the observed relationship between spreading rate and depth to axial low velocity zones, thought to be magma chambers, seismically imaged at active mid-ocean ridges. This was a deliberate strategy to reduce the drilling distance to gabbroic rocks because thick sequences of lavas and dikes have proved difficult to penetrate in past. ODP Leg 206 (2002) initiated operations at Site 1256, including the installation in Hole 1256D of a reentry cone with 16 inch casing inserted through the 250 m thick sedimentary cover and cemented into basement to facilitate deep drilling. The hole was then cored ~500 m into basement. IODP Expeditions 309 and 312 (2005) successfully completed the first sampling of an intact section of upper oceanic crust from lavas, through the sheeted dikes, and into the upper gabbros. Hole 1256D now penetrates >1500 meters below seafloor (mbsf) and >1250 m subbasement and currently resides in the dike–gabbro transition zone. The first gabbroic rocks were encountered at 1407 mbsf. Below this lies a ~100 m complex zone of fractionated gabbros intruded into contact metamorphosed dikes. Although previous cruises achieved the benchmark objective of reaching gabbro in intact ocean crust, critical scientific questions remain. These include the following: 1. Does the lower crust form by the recrystallization and subsidence of a high-level magma chamber (gabbro glacier), crustal accretion by intrusion of sills throughout the lower crust, or some other mechanism? 2. Is the plutonic crust cooled by conduction or hydrothermal circulation? 3. What is the geological nature of Layer 3 and the Layer 2/3 boundary at Site 1256? 4. What is the magnetic contribution of the lower crust to marine magnetic anomalies? Hole 1256D is poised at a depth where samples that should conclusively address these questions can be obtained, possibly with only a few hundred meters of drilling. Importantly, as of the end of Expedition 312, the hole was clear of debris and open to its full depth. Increased rates of penetration (1.2 m/h) and enhanced core recovery (>35%) in the gabbros indicate that this return to Hole 1256D could deepen the hole >300 m into plutonic rocks, past the transition from dikes to gabbro, and into a region of solely cumulate gabbroic rocks.
https://hal.archives-ouvertes.fr/hal-00564953

hal-00564953
https://hal.archives-ouvertes.fr/hal-00564953
DOI : 10.2204/iodp.sp.335.2010

Éditeur(s) : HAL CCSD
Résumé : International audience
This article aims to explore the bilingual practices (English and French) used by students from St Martin in a collaborative situation, in order to enrich the debate on languages and knowledge. This research is based on two corpora consisting of interactions between high school pupils during their educational support sessions, and which are studied using conversational analysis; the study uses this combination of collaborative methods and bilingual education to model the management of linguistic resources with regard to the factual knowledge and practical skills brought into play during the interaction. The challenge of this study is therefore to establish what is involved, and under which conditions and through which mechanisms the interactions between two languages (English and French) may contribute to the construction of cognitive learning skills.
Francisola : : revue indonésienne de la langue et de la littérature françaises

hal-01532983
https://hal.archives-ouvertes.fr/hal-01532983

Éditeur(s) : HAL CCSD Nature Publishing Group
Résumé : International audience
Reactivation of structures inherited from previous collisional or rifting events, especially lithospheric-scale faults, is a major feature of plate tectonics. Its expression ranges from continental break-up along ancient collisional belts(1,2) to linear arrays of intraplate magmatism and seismicity(3,4). Here we use multiscale numerical models to show that this reactivation can result from an anisotropic mechanical behaviour of the lithospheric mantle due to an inherited preferred orientation of olivine crystals. We explicitly consider an evolving anisotropic viscosity controlled by the orientation of olivine crystals in the mantle. We find that strain is localized in domains where shear stresses on the inherited mantle fabric are high, and that this leads to shearing parallel to the inherited fabric. During rifting, structural reactivation induced by anisotropy results in oblique extension, followed by either normal extension or failure. Our results suggest that anisotropic viscosity in the lithospheric mantle controls the location and orientation of intraplate deformation zones that may evolve into new plate boundaries, and causes long-lived lithospheric-scale wrench faults, contributing to the toroidal component of plate motions on Earth.
ISSN: 1752-0894

hal-00413033
https://hal.archives-ouvertes.fr/hal-00413033
DOI : 10.1038/ngeo528

Éditeur(s) : HAL CCSD Cambridge University Press (CUP)
Résumé : International audience
Leishmania parasites can escape the immune response by invading cell types lacking leishmanicidal mechanisms. Silent persistence of Leishmania parasites in the host organism is responsible for asymptomatic carriage and relapses after cured leishmaniasis. Here, we studied the interaction between Hepatic Stellate Cells (HSC) and Leishmania. An original model of human HSC in primary culture infected with L. donovani was developed. The presence of intracellular parasites was studied and quantified using optical and confocal microscopy. HSC characteristics were studied using microscopy, methylene blue assay, long-term cultures and qPCR. We showed for the first time that human HSC are permissive to L. donovani infection, with no modification of HSC survival, growth rate and proinflammatory and fibrogenic characteristics. Intracellular parasites did not replicate but HSC had no effect on their survival. Indeed, after a 40-day culture, infected HSC cultures transferred on NNN medium yielded new promastigotes that were able to proliferate and efficiently infect new cells. HSC are permissive to L. donovani, with neither parasite killing nor apparent cell damage. Thus, HSC could act as potent sanctuary cells for Leishmania in the liver, which could partially explain parasite reactivation after an asymptomatic carriage or a cured visceral leishmaniasis.
ISSN: 0031-1820

inserm-00825803
http://www.hal.inserm.fr/inserm-00825803
DOI : 10.1017/S0031182012001965
PUBMED : 23253952

Éditeur(s) : HAL CCSD Oxford University Press
Résumé : International audience
Type IB DNA topoisomerases can eliminate torsional stresses produced during replication and transcription. These enzymes are found in all eukaryotes and a short version is present in some bacteria and viruses. Among prokaryotes, the long eukaryotic version is only observed in archaea of the phylum Thau-marchaeota. However, the activities and the roles of these topoisomerases have remained an open question. Here, we demonstrate that all available thaumar-chaeal genomes contain a topoisomerase IB gene that defines a monophyletic group closely related to the eukaryotic enzymes. We show that the topIB gene is expressed in the model thaumarchaeon Ni-trososphaera viennensis and we purified the recom-binant enzyme from the uncultivated thaumarchaeon Candidatus Caldiarchaeum subterraneum. This enzyme is active in vitro at high temperature, making it the first thermophilic topoisomerase IB characterized so far. We have compared this archaeal type IB enzyme to its human mitochondrial and nuclear counterparts. The archaeal enzyme relaxes both negatively and positively supercoiled DNA like the eukaryotic enzymes. However, its pattern of DNA cleavage specificity is different and it is resistant to camptothecins (CPTs) and non-CPT Top1 inhibitors, LMP744 and lamellarin D. This newly described ther-mostable topoisomerases IB should be a promising new model for evolutionary, mechanistic and structural studies.
ISSN: 0305-1048

Droits : http://creativecommons.org/licenses/by/
hal-01327354
http://hal.upmc.fr/hal-01327354
http://hal.upmc.fr/hal-01327354/document
http://hal.upmc.fr/hal-01327354/file/Nucl.%20Acids%20Res.-2016-Dahmane-2795-805.pdf
DOI : 10.1093/nar/gkw097