Éditeur(s) : Saint Lucia. Ministry of Social Transformation, Culture & Local Government Saint Lucia. Ministry of Social Transformation, Culture & Local Government
Résumé : (Funding) Digitized with funding from the Digital Library of the Caribbean grant awarded by TICFIA.

Droits : All rights reserved by the source institution.
CULTURAL POLICY

Éditeur(s) : CARDI : Caribbean Agricultural Rechearch and Development Institute Université des Antilles. Service commun de la documentation
Extrait de : 52e congrès annuel de la Société caribéenne des plantes alimentaires / 52nd annual meeting of the Caribbean food crops society (CFCS), du 10 au 16 juillet 2016. INRA, CFCS
Description : Banana and plantain (Musa spp) are important foreign exchange earners in Guyana and the Windward Islands of the Caribbean. Black Sigatoka Disease (BSD) also referred to as black leaf streak disease is one of the most destructive diseases of banana and plantain and is present within the region. In Guyana within two years following its introduction in 2008, the industry was totally decimated, recording a 100% decline, turning the country to a net banana importer. Also, from 2009 ? 2012 the disease spread in the Windward Islands causing as much as 100% decrease in the export of banana. The disease is caused by the ascomycete fungus, Mycosphaerella fijiensis (anamorph Pseudocercospora fijiensis) and is aggressive, challenging to control and results in enormous crop damage if left uncontrolled. In an effort to develop an integrated management approach to the disease the Caribbean Agricultural Research and Development Institute (CARDI) with financial assistance from the Caribbean Development Bank (CDB) is evaluating five FHIA banana and plantain accessions and eight IITA plantain accessions for tolerance to BSD. The evaluation is presently conducted in four Caribbean countries, Dominica, Guyana, St Lucia and St Vincent and the Grenadines. In each country, four distinct agro- ecological zones were selected for the trial evaluation blocks and using a randomized block design, four blocks were established in each country. Five FHIA accessions: FHIA-01, FHIA-03, FHIA-18, FHIA-21 and FHIA-23 are being evaluated in Dominica, St Lucia and St Vincent and the Grenadines whereas, plantain accessions: PITA-17, PITA-21, PITA-22, PITA-23, PITA-24 and PITA-26 are to be evaluated in Guyana for tolerance to the disease. Cavendish are used as regional controls and as borders rows to increase disease pressure. The disease parameters evaluated are youngest leaf spotted (YLS), disease development time (DDT), leaf emission rate (LER), disease severity at 6 months, bunch emergence and harvest, and index of standing leaves. Agronomic data; days from planting to flowering, bunch weight, number of hands in bunch, average weight of hands and average number of fingers per hand and bunch, as well as organoleptic characteristics of both cooked and ripe fruits are also evaluated. The early data collection, of the FHIA only accessions, show trends in the disease tolerance and agronomic performance as well as their adaptability in the different agro-ecological zone. The trends indicate better disease tolerance of FHIA varieties with higher leaf counts before, at bunching and at harvest. The organoleptic test shows a preference to FHIA 03 and FHIA 21. La banane et le plantain (espèces de Musa) sont les acquéreurs de devises étrangères importants en Guyane et en Îles du Vent des Caraïbe. La maladie noire de Sigatoka (schéma) également désignée sous le nom de la maladie noire de filet de feuille est l'une des maladies les plus destructives de la banane et du plantain et est présente dans la région. En Guyane dans un délai de deux ans après son introduction en 2008, l'industrie a été totalement décimée, enregistrant une baisse de 100%, tournant le pays à un importateur net de banane. En outre, à partir de 2009-2012 la propagation de maladie en Îles du Vent causant pas moins de la diminution de 100% de l'exportation de la banane. La maladie est provoquée par le champignon d'ascomycète, fijiensis de Mycosphaerella (fijiensis anamorph de Pseudocercospora) et est agressive, contestant pour commander et les résultats dans l'énorme culture endommagent si laissé incontrôlé. Dans un effort de développer une approche intégrée de gestion à la maladie l'institut agricole des Caraïbes de recherche et développement (CARDI) avec l'aide financière de la banque de développement des Caraïbes (BDC) évalue des adhésions de banane et de plantain de cinq FHIA et huit adhésions de plantain d'IITA pour la tolérance au schéma. L'évaluation est actuellement effectuée quatre pays des Caraïbes, Dominique, Guyane, St Lucia et Saint-Vincent-et-les Grenadines. Dans chaque pays, quatre agro zones écologiques distinctes ont été sélectionnées pour les blocs d'essai d'évaluation et employant une conception de bloc randomisé, quatre blocs ont été établis dans chaque pays. Cinq adhésions de FHIA : FHIA-01, FHIA-03, FHIA-18, FHIA-21 et FHIA-23 sont évalués en Dominique, au St Lucia et au Saint-Vincent-et-les Grenadines tandis que, des adhésions de plantain : PITA-17, PITA-21, PITA-22, PITA-23, PITA-24 et PITA-26 doivent être évalués en Guyane pour la tolérance à la maladie. Cavendish sont employés en tant que contrôles régionaux et comme rangées de frontières pour augmenter la pression de la maladie. Les paramètres de la maladie évalués sont la plus jeune feuille repérée (YLS), le temps d'élaboration de la maladie (DDT), le taux d'émission de feuille (LER), la sévérité de la maladie à 6 mois, émergences et récoltes de groupe, et index des feuilles debout. Données agronomiques ; des jours de la plantation à la floraison, du poids de groupe, nombre de mains dans le poids de groupe et moyen des mains et du nombre moyen de doigts par main et groupe, aussi bien que les caractéristiques organoleptiques des fruits cuits et mûrs sont également évalués. La collecte de données tôt, des adhésions de FHIA seulement, exposition tend dans la tolérance de maladie et la représentation agronomique aussi bien que leur adaptabilité dans la zone agro-écologique différente. Les tendances indiquent qu'une meilleure tolérance de maladie des variétés de FHIA avec une plus haute feuille compte avant, à se rassembler et à la récolte. L'essai organoleptique montre une préférence à FHIA 03 et à FHIA 21.
Siècle(s) traité(s) : 21
Droits : CC-BY-NC-ND - Attribution - Pas d'utilisation commerciale - Pas de modification
Permalien : http://www.manioc.org/fichiers/V16266
V16266

Éditeur(s) : HAL CCSD
Résumé : International audience
STUDY QUESTION: Do freezing and in vitro culture procedures enhance the expression of proteins involved in apoptotic or autophagic pathways in murine pre-pubertal testicular tissue? SUMMARY ANSWER: IVM strongly modified apoptosis- and autophagy-related relative protein levels in mice testicular tissue whereas the impact of cryopreservation procedures was minimal at the end of the culture. WHAT IS KNOWN ALREADY: In vitro spermatogenesis remains a challenging technical issue as it imposes to find a very close balance between survival and death of germ cell natural precursors (i.e. gonocytes and spermatogonia), which will eventually undergo a complete spermatogenesis close to in vivo conditions. The establishment of efficient culture conditions coupled with suitable cryopreservation procedures (e.g. controlled slow freezing [CSF] and solid surface vitrification [SSV]) of pre-pubertal testicular tissue is a crucial step in the fields of fertility preservation and restoration to improve the spermatic yield obtained in vitro. STUDY DESIGN, SIZE, DURATION: Here, we study cryopreservation procedures (i.e. CSF or SSV) and the impact of culture media compositions. A first set of 66 mouse pre-pubertal testes were directly cultured during 30, 36, 38 and 60 days (D) from 2.5 to 6.5-day-old CD-1 mice to evaluate the impact of time-aspect of culture and to endorse the reverse phase protein microarrays (RPPM) technique as an adapted experimental tool for the field of in vitro spermatogenesis. Ninety others fresh, slow-frozen and vitrified pre-pubertal testes were cultured during 30 days for the principal study to evaluate the impact of cryopreservation procedures before and after culture. Thirty-four testes dissected from 2.5, 6.5, 36.5, 40.5, 42.5 and 62.5 days postpartum (dpp) mice, corresponding to the time frames of spermatogenesis orchestrated in vitro, were used as in vivo controls. PARTICIPANTS/MATERIALS, SETTING, METHODS: After in vitro culture, testicular tissue samples originated from 2.5 or 6.5-day-old CD-1 male mice were analyzed using RPPM. This targeted proteomic technique allowed us to assess the expression level of 29 apoptosis- and autophagy-related factors by normalizing blank-corrected signal values. In addition, morphological analyses (e.g. HES, PAS, TRA98 and CREM) and DNA fragmentation in intra-tubular cells (i.e. terminal deoxynucleotidyl transferase dUTP nick end labeling; TUNEL) were assessed for the distinct experimental conditions tested as well as for in vivo control mouse testes. MAIN RESULTS AND THE ROLE OF CHANCE: A validation of the RPPM procedure in the field of in vitro spermatogenesis was completed with assay and array robustness before a principal study concerning the evaluation of the impact of in vitro culture and cryopreservation procedures. The proportion of elongated spermatids and the total cell number per seminiferous tubule tended to be very different between the in vivo and in vitro conditions (P < 0.05), suggesting the presence of a beneficial regulation on the first spermatogenesis wave by intrinsic apoptosis (Caspase₉) and autophagy (Atg5) factors (P < 0.0003 and r2 = 0.74). Concerning the impact of culture media compositions, a basic medium (BM) composed of αMEM plus 10% KnockOut™ serum replacement and gentamicin supplemented with retinol (Rol) and vitamin E (Vit. E) was selected as the best culture medium for fresh 6.5 dpp tissue cultured during 30D with 27.7 ± 8.10% of seminiferous tubules containing elongated spermatids. Concerning the impact of cryopreservation procedures, SSV did not have any impact on the morphological parameters evaluated after culture in comparison to fresh tissue (FT) controls. The proportion of tubules with elongated spermatids on testicular explants cultured with BMRol+Vit. E was not different between SSV (6.6 ± 1.6%) and CSF (5.3 ± 1.9%); however, round spermatids were observed more frequently for SSV (19 ± 6.2%) than CSF (3.3 ± 1.9%, P = 0.0317). Even if the proportion of TUNEL-positive cells for BMRol+Vit. E was higher at D30 after SSV (4.12 ± 0.26%) than CSF (1.86 ± 0.12%, P = 0.0022) and FT (2.69 ± 0.33%, P = 0.0108), the DNA damages observed at the end of the culture (i.e. D30) were similar to respective 6.5 dpp controls. In addition, the relative protein level expression ratio of an apoptotic factor, the phosphorylated FADD on Fas, was reduced by 64-fold in vitrified testes cultured with BMRol+Vit. E. Furthermore, we found in this study that the StemPro®-34 SFM culture medium supplemented with growth factors (e.g. EGF, bFGF, GDNF and LIF) prevented the differentiation of spermatogonial stem cells in favor of a significant proliferation with a better architectural pattern than in vivo 6.5 dpp controls with an increase of seminiferous tubules area for FT (P = 0.0357) and CSF (P = 0.0317). LIMITATIONS REASONS FOR CAUTION: Despite our promising results, the evaluation of apoptotic- and autophagic-related proteins was studied for a limited amount of proteins and on global testicular tissue. WIDER IMPLICATIONS OF THE FINDINGS: The data presented herein will help to improve apoptotic and autophagic understanding during the first spermatogenic wave. Moreover, our findings illustrate for the first time that, using finely-tuned experimental conditions, a testicular in vitro culture combined with proteomic technologies may significantly facilitate the study of cryopreservation procedures and in vitro culture evaluations. This study may also contribute to improve work on testicular tissues from pre-pubertal and adolescent cancer survivors. STUDY FUNDING/COMPETING INTEREST(S): This study was supported by a Ph.D. grant from the Rouen Normandie Université and a financial support from 'la Ligue nationale contre le cancer' (both awarded to L.D.), funding from Institute for Research and Innovation in Biomedicine (IRIB), Agence de la Biomédecine, and co-supported by European Union and Région Normandie. Europe gets involved in Normandie with European Regional Development Fund (ERDF). The authors declare that there is no conflict of interest.
Molecular Human Reproduction

hal-01647081
https://hal-univ-rennes1.archives-ouvertes.fr/hal-01647081
DOI : 10.1093/molehr/gax054
PUBMED : 29040674

Éditeur(s) : Latin American and Caribbean Center at Florida International University Latin American and Caribbean Center at Florida International University ( Miami, Florida )
Résumé : Olsen Jean Julien, Owner and Lead Architect, Phenixience Architecture and Engineering Firm, Haiti, Former Manager, Smithsonian Cultural Recovery Center, Haiti & Former Minister of Culture and Communication, Haiti This commissioned presentation examines Haitian architecture, and in particular, the development, evolution and value of traditional architecture of the 19th century. Additionally, Jean Julien examines issues related to the vulnerability of Haiti's architecture and the importance of preserving it as part of Haiti's cultural patrimony . Part of LACC's 15th Annual Haitian Summer Institute Lecture Series. Part of LACC's Haitian Art Digital Archive.
Due to technical difficulties, this presentation is available in progress from 12 minutes and 54 seconds.
Part of the Latin American and Caribbean Center in the School of International and Public Affairs at Florida International University's Foreign Languages Across the Curriculum Lecture Series.
Haiti

Droits : All rights reserved by the source institution.
http://ufdc.ufl.edu/AA00013163/00001

Éditeur(s) : AIHP-GEODE : Archéologie Industrielle, Histoire, Patrimoine/Géographie- Développement Environnement de la Caraïbe Association des Amis de l
Extrait de : "Chemins croisés de l'Inde" : conférence, le 3 mai 2016. Université des Antilles
Description : Augustin Brutus Jaykumar (Directeur et fondateur de l'Intercultural Network for Development and Peace -INDP) présente une réflexion sur son itinéraire, la construction de sa double culture (Indien et Français) et propose son regard sur sa culture, la reconnaissance de sa propre culture lorsqu'on est à l'extérieur et l'ouverture sur d'autres cultures. Il s'insiste sur le métissage et les valeurs universelles, propose de s'enraciner intelligemment en reconnaissant son passé en le mettant au service de l'endroit où l'on vit.

Droits : CC-BY-NC-ND - Attribution - Pas d'utilisation commerciale - Pas de modification
Permalien : http://www.manioc.org/fichiers/V16115
V16115

Éditeur(s) : Dominica. Minstry of Community Development, Gender Affairs and Information Dominica. Minstry of Community Development, Gender Affairs and Information
Résumé : (Funding) Support for the development of the technical infrastructure and partner training provided by the United States Department of Education TICFIA program.

Droits : All rights reserved by the source institution.
CULTURAL POLICY

Éditeur(s) : INRA : Institut National de la Recherche Agronomique Université des Antilles. Service commun de la documentation
Extrait de : 52e congrès annuel de la Société caribéenne des plantes alimentaires / 52nd annual meeting of the Caribbean food crops society (CFCS), du 10 au 16 juillet 2016. INRA, CFCS
Description : Plant genetic resources are instrumental in the adaptation of agriculture to social and environmental change. They are the backbone of research and breeding programs aimed at the development and transfer of new crop varieties best suited to consumers? needs and tastes and to new farming systems. To this aim, plant germplasm collections have been constituted worldwide. Securing such collections requires substantial human and financial investments that can prove difficult to maintain on the long run for small countries and territories such as most Caribbean countries. In Guadeloupe and Martinique, INRA and CIRAD have constituted large plant germplasm collections of tropical crops over several decades. They joined forces in 2010 to create the Tropical Plant Biological Resource Center of the French West Indies (CRB-PT), which is affiliated to both institutions. In this paper, we describe CRB-PT?s collections, services provided to end users and research programs as well as scientific and technical networking strategy. Les ressources génétiques d'usine sont instrumentales dans l'adaptation de l'agriculture au changement social et environnemental. Elles sont l'épine dorsale de la recherche et les programmes d'élevage ont visé le développement et le transfert de nouvelles variétés de culture adaptées aux besoins et aux goûts des consommateurs et à de nouveaux systèmes d'exploitation agricole. À ce but, des collections de matériel génétique d'usine ont été constituées dans le monde entier. La fixation de telles collections exige les investissements humains et substantiels qui peuvent être difficile à maintenir sur le long terme pour de petits pays et territoires tels que la plupart des pays des Caraïbes. En Guadeloupe et Martinique, l'AICN et les CIRAD ont constitué de grandes collections de matériel génétique d'usine de cultures tropicales au-delà de plusieurs décennies. Ils ont joint des forces en 2010 pour créer le centre de ressources biologique de plante tropicale des Antilles françaises (CRB-PT), qui sont affiliées aux deux établissements. En ce document, nous décrivons les collections de CRB-PT, services fournis aux utilisateurs et les programmes de recherche aussi bien que la stratégie scientifique et technique de mise en réseau.
Siècle(s) traité(s) : 21
Droits : CC-BY-NC-ND - Attribution - Pas d'utilisation commerciale - Pas de modification
Permalien : http://www.manioc.org/fichiers/V16258
V16258

Éditeur(s) : CIRAD : Centre de Coopération Internationale en Recherche Agronomique pour le Développement
Extrait de : 52e congrès annuel de la Société caribéenne des plantes alimentaires / 52nd annual meeting of the Caribbean food crops society (CFCS), du 10 au 16 juillet 2016. INRA, CFCS
Description : Breeding new interspecific banana hybrid varieties with pests and disease resistance characters is instrumental for the development of pesticide-free agroecological banana farming systems. Such breeding relies on the combined use of Musa acuminata and M. balbisiana parents. Unfortunately, infectious alleles of endogenous Banana streak virus (eBSV) sequences are present in the genome of M. balbisiana genitors. Upon activation by biotic and abiotic stresses, these infectious eBSVs lead to spontaneous infections by several species of Banana streak virus (BSV) in interspecific hybrids harbouring both M. acuminata and M. balbisiana genomes. In this work, we show that M. balbisiana diploid genitors available for breeding host at least one infectious eBSV. We also show how we segregated infectious and non-infectious eBSV alleles in seedy M. balbisiana diploids through self- pollination or chromosome doubling of haploid lines. We report on the successful breeding of M. balbisiana diploid genitors devoid of all infectious eBSV alleles following self-pollination and on the potential of breeding additional M. balbisiana diploid genitors free of infectious eBSVs by crossing parents displaying complementary eBSV patterns. This work paves the way to the safe use of M. balbisiana genitors for breeding banana interspecific hybrid varieties with no risk of activation of infectious eBSVs.
Siècle(s) traité(s) : 21
Droits : CC-BY-NC-ND - Attribution - Pas d'utilisation commerciale - Pas de modification
Permalien : http://www.manioc.org/fichiers/V16267
V16267

Éditeur(s) : Latin American and Caribbean Center at Florida International University Latin American and Caribbean Center at Florida International University ( Miami, Florida )
Résumé : Olsen Jean Julien, Owner and Lead Architect, Phenixience Architecture and Engineering Firm, Haiti, Former Manager, Smithsonian Cultural Recovery Center, Haiti & Former Minister of Culture and Communication, Haiti This commissioned presentation examines Haitian architecture, and in particular, the development, evolution and value of traditional architecture of the 19th century. Additionally, Jean Julien examines issues related to the vulnerability of Haiti's architecture and the importance of preserving it as part of Haiti's cultural patrimony . Part of LACC's 15th Annual Haitian Summer Institute Lecture Series. Part of LACC's Haitian Art Digital Archive.
Due to technical difficulties, this presentation is available in progress from 12 minutes and 54 seconds.
Part of the Latin American and Caribbean Center in the School of International and Public Affairs at Florida International University's Foreign Languages Across the Curriculum Lecture Series.
Haiti

Droits : All rights reserved by the source institution.
http://ufdc.ufl.edu/AA00013163/00001

Éditeur(s) : University of the West Indies Cave Hill/University of Reading University of the West Indies Cave Hill/University of Reading ( Barbados )
Résumé : (Funding) Support for the development of the technical infrastructure and partner training provided by the United States Department of Education TICFIA program.
Outline from the website at http://www.reading.ac.uk/minorities/Projects/min-project-breaking-sexual-silences.aspx In recent years, some of the most urgent and highly-charged public and political debates in the Caribbean have centred on sexual citizenship and gay rights. To date, popular cultural forms and the acute homophobia of the dancehall have dominated national and international attention. The very public exchanges between Caribbean musicians and western-based gay rights campaigners have given the region a reputation for homophobia, intolerance and hate crimes. This project seeks to shift the axes of these debates by drawing on a recent body of Caribbean creative writing that addresses issues of sexual self-determination and sexual diversity in a more positive and progressive way. It will make visible the possibilities for understanding sexual differences and the modes of reconciliation to be found in a literary archive. The project will also engage a group of Caribbean scholars across other disciplines to develop a new language for articulating sexual difference. It will stage a panel debate for a Caribbean public, as well as producing academic publications and supporting graduate work.

Droits : All rights reserved by the source institution.

Éditeur(s) : Breaking Sexual Silences Project - University of Reading/ University of West Indies Breaking Sexual Silences Project - University of Reading/ University of West Indies ( Barbados )
Résumé : (Funding) Support for the development of the technical infrastructure and partner training provided by the United States Department of Education TICFIA program.
In recent years, some of the most urgent and highly-charged public and political debates in the Caribbean have centred on sexual citizenship and gay rights. To date, popular cultural forms and the acute homophobia of the dancehall have dominated national and international attention. The very public exchanges between Caribbean musicians and western-based gay rights campaigners have given the region a reputation for homophobia, intolerance and hate crimes. This project seeks to shift the axes of these debates by drawing on a recent body of Caribbean creative writing that addresses issues of sexual self-determination and sexual diversity in a more positive and progressive way. It will make visible the possibilities for understanding sexual differences and the modes of reconciliation to be found in a literary archive. The project will also engage a group of Caribbean scholars across other disciplines to develop a new language for articulating sexual difference. It will stage a panel debate for a Caribbean public, as well as producing academic publications and supporting graduate work.

Droits : All rights reserved by the source institution.

Éditeur(s) : Fundación Global Democracia y Desarrollo (FUNGLODE) Fundación Global Democracia y Desarrollo (FUNGLODE)
Résumé : (Funding) Support for the development of the technical infrastructure and partner training provided by the United States Department of Education TICFIA program.
South America -- Dominican Republic

Droits : All rights reserved by the source institution.
99934-0-355-5

Éditeur(s) : Fundación Global Democracia y Desarrollo Fundación Global Democracia y Desarrollo
Résumé : (Funding) Support for the development of the technical infrastructure and partner training provided by the United States Department of Education TICFIA program.
Europe
South America
North America

Droits : All rights reserved by the source institution.
99934-0-359-8

Éditeur(s) : University of the West Indies Cave Hill/University of Reading University of the West Indies Cave Hill/University of Reading ( Barbados )
Résumé : (Funding) Support for the development of the technical infrastructure and partner training provided by the United States Department of Education TICFIA program.
Outline In recent years, some of the most urgent and highly-charged public and political debates in the Caribbean have centred on sexual citizenship and gay rights. To date, popular cultural forms and the acute homophobia of the dancehall have dominated national and international attention. The very public exchanges between Caribbean musicians and western-based gay rights campaigners have given the region a reputation for homophobia, intolerance and hate crimes. This project seeks to shift the axes of these debates by drawing on a recent body of Caribbean creative writing that addresses issues of sexual self-determination and sexual diversity in a more positive and progressive way. It will make visible the possibilities for understanding sexual differences and the modes of reconciliation to be found in a literary archive. The project will also engage a group of Caribbean scholars across other disciplines to develop a new language for articulating sexual difference. It will stage a panel debate for a Caribbean public, as well as producing academic publications and supporting graduate work.

Droits : All rights reserved by the source institution.

Éditeur(s) : HAL CCSD American Physiological Society
Résumé : Full text: http://jn.physiology.org/content/88/3/1475
International audience
The contribution of voltage-gated calcium channels (VGCC) to the development of cultured embryonic cockroach brain neurons was assessed using pharmacological agents. VGCC currents were recorded using the patch-clamp technique and were found to be blocked dose-dependently by micromolar concentrations of mibefradil. The activation and inactivation properties of the calcium channels enable a sizeable calcium current to flow at rest (about -30 and -20 mV in high-potassium culture media). As expected, the cytoplasmic-free calcium concentration was found to rise when the extracellular potassium concentration was raised from 3 to 15 and 30 mM. The effects of VGCC blockers and calcium chelators were different in fresh and in mature cultures in which the neurons were connected to each other to form a defined network. In fresh cultures, the two non-selective VGCC blockers (verapamil and mibefradil) induced a dose-dependent cell death that was proportional to their blocking effect on I(Ba). This effect could not be prevented by addition of fetal calf serum to the culture medium. A similar effect was obtained using intra- or extracellular calcium chelating agents (10 microM BAPTA-AM or 10 mM EGTA). Quite unexpectedly, blockade of the P/Q-like (omega-Aga WA-sensitive) component of the calcium current by 500 nM of omega-AgaTx IVA had no lethal effect, suggesting that the corresponding channels are not involved in the survival mechanism. As expected from their lack of effect on I(Ba), isradipine, nifedipine, and omega-CgTx GVIA did not induce cell death. When the neurons started growing neurites, their sensitivity to calcium channel blockade by mibefradil decreased, indicating a correlation between neurite outgrowth and resistance to calcium depletion. In mature cultures, the neurons became resistant to mibefradil, verapamil, and BAPTA-AM. However, these agents, as well as omega-AgaTx IVA, had a significant inhibitory effect on the increase in diameter of the connectives that linked adjacent clusters of neurons. This effect has been shown to result, in the case of mibefradil, from an inhibition of neurite outgrowth characterized by a significant reduction of the number of primary neurites and secondary branchings but not to a significant modification of the diameter of individual neurites. These results support the view that, as in vertebrates, calcium influx through VGCC plays an important role in survival and neurite outgrowth of cultured embryonic insect neurons. The differential contribution of the P/Q-like and R-like (omega-Aga WA-sensitive) calcium channels in these processes is discussed.
ISSN: 0022-3077

hal-00982287
https://hal.archives-ouvertes.fr/hal-00982287
DOI : 10.1152/jn.00749.2001
PUBMED : 12205168

Éditeur(s) : Breaking Sexual Silences Project - University of Reading/ University of West Indies Breaking Sexual Silences Project - University of Reading/ University of West Indies ( Barbados )
Résumé : (Funding) Support for the development of the technical infrastructure and partner training provided by the United States Department of Education TICFIA program.
In recent years, some of the most urgent and highly-charged public and political debates in the Caribbean have centred on sexual citizenship and gay rights. To date, popular cultural forms and the acute homophobia of the dancehall have dominated national and international attention. The very public exchanges between Caribbean musicians and western-based gay rights campaigners have given the region a reputation for homophobia, intolerance and hate crimes. This project seeks to shift the axes of these debates by drawing on a recent body of Caribbean creative writing that addresses issues of sexual self-determination and sexual diversity in a more positive and progressive way. It will make visible the possibilities for understanding sexual differences and the modes of reconciliation to be found in a literary archive. The project will also engage a group of Caribbean scholars across other disciplines to develop a new language for articulating sexual difference. It will stage a panel debate for a Caribbean public, as well as producing academic publications and supporting graduate work.

Droits : All rights reserved by the source institution.

Éditeur(s) : UNDP Guyana UNDP Guyana ( Guyana )
Résumé : (Funding) Support for the development of the technical infrastructure and partner training provided by the United States Department of Education TICFIA program.
While the global response to HIV and AIDS has realized several successes in achieving universal access to prevention, care and treatment to date, challenges persist in ensuring equal access to these services for most at risk populations. The epidemiology of HIV reflects a stark disparity in access to prevention and treatment services for neglected most-at risk populations, such as men who have sex with men (MSM). World-wide, MSM often experience higher rates of HIV relative to the total population. The reasons for this dynamic are multi-faceted and include high risk behaviors, and cultural as well as structural barriers. For MSM in developing countries, for example, basic services for prevention and treatment of HIV infection have yet to reach the large majority of men. Homophobia and discrimination limit access of MSM to prevention services and markedly increase vulnerability, as do criminalization of same-sex behavior. Decriminalization of same-sex behavior is a structural intervention for prevention of HIV infection and has recently been embraced by a nonbinding statement from the United Nations (Beyrer C,Clin Infect Dis. 2010 May 15;50 Suppl 3:S108-13) In the Caribbean, all of the above mentioned barriers exist. In fact, recent evidence demonstrates that there is a correlation between the decriminalization of homosexuality and lower rates of HIV (UNAIDS). This correlation is attributed to improved access to services. Therefore, The United Nations Development Program (UNDP) has recently launched an initiative to address the current situation regarding men who have sex with men in Guyana where the HIV prevalence in this population is 19.4% in contrast to 1.8% in the total population. This rapid assessment has been commissioned to supplement two previous studies that were conducted in Guyana in informing the design of upcoming activities to support local capacity building to enhance access to services and respect for the human rights protections for MSM. Unfortunately, in Guyana, like much of the Caribbean, MSM tend to be a hard to reach population, perhaps due to elevated levels of stigma and discrimination and difficulty in accessing men who might participate in high risk same sex behaviors yet do not self-identify as an MSM. As a result, there is a relative paucity of data concerning the knowledge, attitudes and behaviors of this total population in Guyana. (i.e. those who self –identify and those who do not) The baseline report is organized as follows: the methodology is explained followed by the study limitations, the review (literature and desk). The report then proceeds with describing the reality of work and working with sexual and gender minorities in Guyana, the status quo and entry points. The main findings of the capacity assessment and focus groups are then discussed. Issues relating to the uniformed forces and health services providers are then presented followed by discussions and conclusions.

Droits : All rights reserved by the source institution.

Éditeur(s) : HAL CCSD
Résumé : International audience
How do we learn to communicate: how do we come to language or how does language come to us? Would it be a question of adaptation to the world by the act of speaking as suggested by J. Bernicot 1 (1992); would man benefit first and foremost from a genetic patrimony that enabled him to make use of language, as N. Chomsky (1985) points out by asking the existence of a competence related to generative mechanisms? J. Bruner (1996) proposes the idea of an education likely to "adapt a culture to the needs of its members and to adapt its members and their ways of learning to the needs of culture". 2. A / Motivations and objects An approach to mother-child relationships (master's thesis in developmental psychology 3, Miehakanda, 1998) has given me the opportunity to highlight the mediation of play in the structuring of relationships between the child with academic difficulties and his parents. The specific framework of this research-action was that of the educational and therapeutic care of children attending primary school (CE1-CLIS-CLAD) 4. These seven children (three girls / four boys) who were reported for various personality and behavioral disorders all had a lack of self-esteem and self-esteem affecting their relationships with those around them (peers, teachers ...).
Comment apprenons-nous à communiquer : comment venons-nous au langage ou comment le langage vient-il à nous ? Serait-ce une question d'adaptation au monde par l'action de parler comme le suggère J. Bernicot 1 (1992) ; l'homme bénéficierait-il prioritairement d'un patrimoine génétique le disposant à faire usage du langage, comme l'indique N. Chomsky (1985) en posant l'existence d'une compétence reliée à des mécanismes génératifs ? J. Bruner (1996) propose l'idée d'une éducation susceptible «d'adapter une culture aux besoins de ses membres et d'adapter ses membres et leurs manières d'apprendre aux besoins de la culture» 2. A/ Motivations et objets Une approche des relations mères-enfants (mémoire de maîtrise de psychologie du développement 3 ; Miehakanda, 1998) m'a donné l'occasion de mettre en évidence la médiation du jeu dans la structuration des rapports entre l'enfant rencontrant des difficultés scolaires et ses parents. Le cadre spécifique de cette recherche-action était celui des prises en charge éducatives et thérapeutiques d'enfants scolarisés à l'école primaire (CE1-CLIS-CLAD) 4. Ces sept enfants (trois filles / quatre garçons) qui étaient signalés pour diverses formes de troubles de la personnalité et du comportement éprouvaient tous un manque de confiance en soi et d'estime de soi affectant leurs rapports avec leur entourage (pairs ; enseignants…).
https://hal.univ-antilles.fr/tel-01658934

ISBN : Livre broché : ISBN-10: 3841633412 ISBN-13: 978-3841633415. 2015, pp 316
tel-01658934
https://hal.univ-antilles.fr/tel-01658934

Éditeur(s) : HAL CCSD
Résumé : International audience
A prospective study on randomized patients was conducted to determine how morphokinetic parameters are altered in embryos grown in sequential versus global culture media. Eleven morphokinetic parameters of 160 single embryos transferred were analyzed by time lapse imaging involving two University-affiliated in vitro fertilization (IVF) centers. We found that the fading of the two pronuclei occurred earlier in global (22.56±2.15 hpi) versus sequential media (23.63±2.71 hpi; p=0.0297). Likewise, the first cleavage started earlier at 24.52±2.33 hpi vs 25.76±2.95 hpi (p=0.0158). Also, the first cytokinesis was shorter in global medium, lasting 18±10.2 minutes in global versus 36±37.8 minutes in sequential culture medium (p <0.0001). We also observed a significant shortening in the duration of the 2-cell stage in sequential medium: 10.64 h±2.75 versus 11.66 h±1.11 in global medium (p=0.0225) which suggested a faster progression of the embryos through their first mitotic cell cycle. In conclusion, morphokinetic analysis of human embryos by Time lapse imaging reveals significant differences in five kinetic variables according to culture medium. Our study highlights the need to adapt morphokinetic analysis accordingly to the type of media used to best support human early embryo development.
Annales de Biologie Clinique

hal-01579406
https://hal-univ-rennes1.archives-ouvertes.fr/hal-01579406
DOI : 10.1684/abc.2017.1259
PUBMED : 28751285

Éditeur(s) : HAL CCSD Elsevier
Résumé : International audience
Evidence of a changing environment in the catchment area of a small pond (Lansquenet, Lorraine, France) during the last millennium was provided by coupling palynology data and organic ratios [terrestrial to aquatic ratio: TAR(HC); the C29 vs. C27 sterols, C29/C27(ST); average chain length, ACL and aquatic/macrophytes ratio, Paq]. The depositional and alteration conditions of organic matter (OM) were provided by the 5α(H)-stanols/Δ5-sterols ratio. This study shows that quite simple organic ratios clearly allow identification of the different evolution steps of a watershed, from a swamp to the progressive settlement of a pond. During the swampy period, the C/N(at) (ca. 11.1), TAR(HC) (ca. 36.8), C29/C27(ST) (ca. 8.7) and Paq (<0.3) show a high contribution of allochthonous OM associated with the presence of particular aquatic and mesohygrophilous species. A woody level dated to the XIIIth century reflects the period of the settlement of the pond in a more oxic environment [5α(H)-stanol/Δ5sterol ratio <0.2]. Then, the progressive increase in aquatic input and the development of submerged and floating macrophytes are evidenced by low C/N(at) (<7.6), TAR(HC) (ca. 2.7), C29/C27(ST) (ca. 2) and high Paq (ca. 0.5), suggesting an increase in the water level and eutrophication of the pond. A second oxic event [5α(H)-stanols/Δ5sterols ratio averaging 0.16], associated with extensive OM degradation, corresponds to the most recent dredging in 2002 and reflects an important disturbance of sediments due to pond management.
ISSN: 0146-6380

hal-00758066
https://hal.archives-ouvertes.fr/hal-00758066
DOI : 10.1016/j.orggeochem.2012.06.004