Éditeur(s) : HAL CCSD Landes Bioscience
Résumé : International audience
In 2008 we published the first set of guidelines for standardizing research in autophagy. Since then, research on this topic has continued to accelerate, and many new scientists have entered the field. Our knowledge base and relevant new technologies have also been expanding. Accordingly, it is important to update these guidelines for monitoring autophagy in different organisms. Various reviews have described the range of assays that have been used for this purpose. Nevertheless, there continues to be confusion regarding acceptable methods to measure autophagy, especially in multicellular eukaryotes. A key point that needs to be emphasized is that there is a difference between measurements that monitor the numbers or volume of autophagic elements (e.g., autophagosomes or autolysosomes) at any stage of the autophagic process vs. those that measure flux through the autophagy pathway (i.e., the complete process); thus, a block in macroautophagy that results in autophagosome accumulation needs to be differentiated from stimuli that result in increased autophagic activity, defined as increased autophagy induction coupled with increased delivery to, and degradation within, lysosomes (in most higher eukaryotes and some protists such as Dictyostelium) or the vacuole (in plants and fungi). In other words, it is especially important that investigators new to the field understand that the appearance of more autophagosomes does not necessarily equate with more autophagy. In fact, in many cases, autophagosomes accumulate because of a block in trafficking to lysosomes without a concomitant change in autophagosome biogenesis, whereas an increase in autolysosomes may reflect a reduction in degradative activity. Here, we present a set of guidelines for the selection and interpretation of methods for use by investigators who aim to examine macroautophagy and related processes, as well as for reviewers who need to provide realistic and reasonable critiques of papers that are focused on these processes. These guidelines are not meant to be a formulaic set of rules, because the appropriate assays depend in part on the question being asked and the system being used. In addition, we emphasize that no individual assay is guaranteed to be the most appropriate one in every situation, and we strongly recommend the use of multiple assays to monitor autophagy. In these guidelines, we consider these various methods of assessing autophagy and what information can, or cannot, be obtained from them. Finally, by discussing the merits and limits of particular autophagy assays, we hope to encourage technical innovation in the field.
Autophagy

hal-00735751
https://hal.archives-ouvertes.fr/hal-00735751
PUBMED : 22966490

Résumé : Ce document présente l'état des principaux stocks exploités par les flottes françaises en termes de mortalité par pêche et de biomasse de reproducteurs. La situation fin 2010 (début 2011) par rapport aux points de référence (de précaution et Fmsy) est indiqué lorsque les indicateurs sont estimés et que les références sont définies. Les grandes tendances en matière d'évolution des mortalités et biomasses sont également indiquées.

Droits : 2011 Ifremer
http://archimer.ifremer.fr/doc/00035/14656/11956.pdf
http://archimer.ifremer.fr/doc/00035/14656/

Éditeur(s) : Amer Geophysical Union
Résumé : After the January 12, 2010, Haiti earthquake, we deployed a mainly offshore temporary network of seismologic stations around the damaged area. The distribution of the recorded aftershocks, together with morphotectonic observations and mainshock analysis, allow us to constrain a complex fault pattern in the area. Almost all of the aftershocks have a N-S compressive mechanism, and not the expected left-lateral strike-slip mechanism. A first-order slip model of the mainshock shows a N264 degrees E north-dipping plane, with a major left-lateral component and a strong reverse component. As the aftershock distribution is sub-parallel and close to the Enriquillo fault, we assume that although the cause of the catastrophe was not a rupture along the Enriquillo fault, this fault had an important role as a mechanical boundary. The azimuth of the focal planes of the aftershocks are parallel to the north-dipping faults of the Transhaitian Belt, which suggests a triggering of failure on these discontinuities. In the western part, the aftershock distribution reflects the triggering of slip on similar faults, and/or, alternatively, of the south-dipping faults, such the Trois-Baies submarine fault. These observations are in agreement with a model of an oblique collision of an indenter of the oceanic crust of the Southern Peninsula and the sedimentary wedge of the Transhaitian Belt: the rupture occurred on a wrench fault at the rheologic boundary on top of the under-thrusting rigid oceanic block, whereas the aftershocks were the result of the relaxation on the hanging wall along pre-existing discontinuities in the frontal part of the Transhaitian Belt. Citation: Mercier de Lepinay, B., et al. (2011), The 2010 Haiti earthquake: A complex fault pattern constrained by seismologic and tectonic observations, Geophys. Res. Lett., 38, L22305, doi:10.1029/2011GL049799.
Geophysical Research Letters (0094-8276) (Amer Geophysical Union), 2011-11 , Vol. 38 , N. L22305 , P. 7 p.

Droits : 2011 by the American Geophysical Union
http://archimer.ifremer.fr/doc/00054/16557/14071.pdf
DOI:10.1029/2011GL049799
http://archimer.ifremer.fr/doc/00054/16557/

Éditeur(s) : HAL CCSD BioMed Central
Résumé : International audience
Background: HCV treatment uptake has drastically increased in HIV-HCV coinfected patients in France since direct-acting antiviral (DAA) treatment approval, resulting in HCV cure in 63% of all HIV-HCV patients by the end of 2015. We investigated the impact of scaling-up DAA on HCV prevalence in the whole HIV population and in various risk groups over the next 10 years in France using a transmission dynamic compartmental model.Methods: The model was based on epidemiological data from the French Dat’AIDS cohort. Eight risk groups wereconsidered, including high-risk (HR) and low-risk (LR) men who have sex with men (MSM) and male/female heterosexuals,intra-venous drug users, or patients from other risk groups. The model was calibrated on prevalence and incidence dataobserved in the cohort between 2012 and 2015.Results: On January 1, 2016, 156,811 patients were registered as infected with HIV in France (24,900 undiagnosedpatients) of whom 7938 (5.1%) had detectable HCV-RNA (722 undiagnosed patients). Assuming a treatmentcoverage (TC) rate of 30%/year (i.e., the observed rate in 2015), model projections showed that HCV prevalenceamong HIV patients is expected to drop to 0.81% in 2026. Sub-analyses showed a similar decrease of HIV-HCVprevalence in most risk groups, including LR MSM. Due to higher infection and reinfection rates, predicted prevalencein HR MSM remained stable from 6.96% in 2016 to 6.34% in 2026. Increasing annual TC rate in HR MSM to 50/70%would decrease HCV prevalence in this group to 2.35/1.25% in 2026. With a 30% TC rate, undiagnosed patients wouldaccount for 34% of HCV infections in 2026.Conclusions: Our model suggests that DAA could nearly eliminate coinfection in France within 10 years for most riskgroups, including LR MSM. Elimination in HR MSM will require increased TC.
ISSN: 1741-7015

Droits : info:eu-repo/semantics/OpenAccess
inserm-01685739
http://www.hal.inserm.fr/inserm-01685739
http://www.hal.inserm.fr/inserm-01685739/document
http://www.hal.inserm.fr/inserm-01685739/file/2017%2C%20Virlogeux%20-%20Modeling%20HIV-HCV%20coinfection.pdf
DOI : 10.1186/s12916-017-0979-1

Éditeur(s) : Amer Geophysical Union
Résumé : The origin of the Algerian margin remains one of the key questions still discussed in the Western Mediterranean sea, due to the imprecise nature and kinematics of the associated basin during the Neogene. For the first time, the deep structure of the Maghrebian margin was explored during the SPIRAL seismic survey. In this work, we present a N-S transect off Tipaza (west of Algiers), a place where the margin broadens due to a topographic high (Khayr-al-Din Bank). New deep penetration seismic profiles allow us to image the sedimentary sequence in the Algerian basin and the crustal structure at the continent-ocean boundary. Modeling of the wide-angle data shows thinning of the basement, from more than 15km in the continental upper margin to only 5–6km of oceanic-type basement in the Algerian basin, and reveals a very narrow or absent transitional zone. Analysis of the deep structure of the margin indicates features inherited from its complex evolution: (1) an oceanic-type crust in the deep basin, (2) similarities with margins formed in a transform-type setting, (3) a progressive deepening of the whole sedimentary cover, and the thickening of the Plio-Quaternary sediments at the margin foot, coeval with (4) a downward flexure of the basement in the basin. These features argue for a multiphased evolution of the margin, including (1) an early stage of rifting and/or spreading, (2) a late transcurrent episode related to the westward migration of the Alboran domain, and (3) a diffuse Plio-Quaternary compressional reactivation of the margin.
Journal Of Geophysical Research-solid Earth (0148-0027) (Amer Geophysical Union), 2013-08 , Vol. 118 , N. 8 , P. 3899-3916

Droits : 2013. American Geophysical Union. All Rights Reserved.
http://archimer.ifremer.fr/doc/00152/26333/24412.pdf
DOI:10.1002/jgrb.50318
http://archimer.ifremer.fr/doc/00152/26333/

Éditeur(s) : HAL CCSD Nature Publishing Group
Résumé : International audience
Treacher Collins/Franceschetti syndrome (TCS; OMIM 154500) is a disorder of craniofacial development belonging to the heterogeneous group of mandibulofacial dysostoses. TCS is classically characterized by bilateral mandibular and malar hypoplasia, downward-slanting palpebral fissures, and microtia. To date, three genes have been identified in TCS:,TCOF1, POLR1D, and POLR1C. We report a clinical and extensive molecular study, including TCOF1, POLR1D, POLR1C, and EFTUD2 genes, in a series of 146 patients with TCS. Phenotype-genotype correlations were investigated for 19 clinical features, between TCOF1 and POLR1D, and the type of mutation or its localization in the TCOF1 gene. We identified 92/146 patients (63%) with a molecular anomaly within TCOF1, 9/146 (6%) within POLR1D, and none within POLR1C. Among the atypical negative patients (with intellectual disability and/or microcephaly), we identified four patients carrying a mutation in EFTUD2 and two patients with 5q32 deletion encompassing TCOF1 and CAMK2A in particular. Congenital cardiac defects occurred more frequently among patients with TCOF1 mutation (7/92, 8%) than reported in the literature. Even though TCOF1 and POLR1D were associated with extreme clinical variability, we found no phenotype-genotype correlation. In cases with a typical phenotype of TCS, 6/146 (4%) remained with an unidentified molecular defect.
ISSN: 1098-3600

hal-01134364
https://hal-univ-rennes1.archives-ouvertes.fr/hal-01134364
DOI : 10.1038/gim.2015.29
PUBMED : 25790162

Éditeur(s) : Elsevier
Résumé : We describe for the first time a set of large active thrusts and folds near the foot of the easternmost Algerian margin, Western Mediterranean, from swath bathymetry and high-resolution seismic data acquired in 2005 during the Maradja2/Samra cruise. This active system resumes a previous passive margin and creates growth strata deposition on the limbs of large folds, resulting in the development of perched basins at the foot of the margin since less than similar to 1 Ma. They form a set of overlapping fault segments verging toward the Algerian basin, in a way similar to what has been observed off eastern Algiers on the rupture zone of the 2003 Mw 6.8 Boumerdes earthquake. The horizontal shortening rate across large folds is estimated to be of the order of 1 mm/yr. Although no historical earthquakes are reported here, these fault segments could have been responsible for large (M similar to 7.5) events in the past. This young tectonic system further supports the hypothesis of subduction inception of the Neogene oceanic lithosphere in the context of the Africa-Eurasia convergence.
Marine Geology (0025-3227) (Elsevier), 2009-06 , Vol. 261 , N. 1-4 , P. 17-32

Droits : 2009 Published by Elsevier Inc.
http://archimer.ifremer.fr/doc/2009/publication-6850.pdf
DOI:10.1016/j.margeo.2008.05.016
http://archimer.ifremer.fr/doc/00000/6850/

Éditeur(s) : Pergamon-elsevier Science Ltd
Résumé : The EUNIS (European Union Nature Information System) habitat classification system aims to provide a common European reference set of habitat types within a hierarchical classification, and to cover all terrestrial, freshwater and marine habitats of Europe. The classification facilitates reporting of habitat data in a comparable manner, for use in nature conservation (e.g. inventories, monitoring and assessments), habitat mapping and environmental management. For the marine environment the importance of a univocal habitat classification system is confirmed by the fact that many European initiatives, aimed at marine mapping, assessment and reporting, are increasingly using EUNIS habitat categories and respective codes. For this reason substantial efforts have been made to include information on marine benthic habitats from different regions, aiming to provide a comprehensive geographical coverage of European seas. However, there still remain many concerns on its applicability as only a small fraction of Europe's seas are fully mapped and increasing knowledge and application raise further issues to be resolved. This paper presents an overview of the main discussion and conclusions of a workshop, organised by the MeshAtlantic project, focusing upon the experience in using the EUNIS habitats classification across different countries and seas, together with case studies. The aims of the meeting were to: (i) bring together scientists with exper_ience_in the use of the EUNIS marine classification and representatives from the European Environment Agency (EEA); (ii) agree on enhancements to EUNIS that ensure an improved representation of the European marine habitats; and (iii) establish practices that make marine habitat maps produced by scientists more consistent with the needs of managers and decision-makers. During the workshop challenges for the future development of EUNIS were identified, which have been classified into five categories: (1) structure and hierarchy; (2) biology; (3) terminology; (4) mapping; and (5) future development. The workshop ended with a declaration from the attendees, with recommendations to the EEA and European Topic Centre on Biological Diversity, to take into account the outputs of the workshop, which identify weaknesses in the current classification and include proposals for its modification, and to devise a process to further develop the marine component of the EUNIS habitat classification. (C) 2012 Elsevier Ltd. All rights reserved.
Marine Pollution Bulletin (0025-326X) (Pergamon-elsevier Science Ltd), 2012-12 , Vol. 64 , N. 12 , P. 2630-2638

Droits : 2012 Elsevier Ltd. All rights reserved.
http://archimer.ifremer.fr/doc/00125/23663/22686.pdf
DOI:10.1016/j.marpolbul.2012.10.010
http://archimer.ifremer.fr/doc/00125/23663/

Éditeur(s) : HAL CCSD Royal Society
Résumé : International audience
The 2 465 177 bp genome of Sulfolobus islandicus LAL14/1, host of the model rudivirus SIRV2, was sequenced. Exhaustive comparative genomic analysis of S. islandicus LAL14/1 and the nine other completely sequenced S. islandicus strains isolated from Iceland, Russia and USA revealed a highly syntenic common core genome of approximately 2 Mb and a long hyperplastic region containing most of the strain-specific genes. In LAL14/1, the latter region is enriched in insertion sequences, CRISPR (clustered regularly interspaced short palindromic repeats), glycosyl transferase genes, toxin-antitoxin genes and MITE (miniature inverted-repeat transposable elements). The tRNA genes of LAL14/1 are preferential targets for the integration of mobile elements but clusters of atypical genes (CAG) are also integrated elsewhere in the genome. LAL14/1 carries five CRISPR loci with 10 per cent of spacers matching perfectly or imperfectly the genomes of archaeal viruses and plasmids found in the Icelandic hot springs. Strikingly, the CRISPR_2 region of LAL14/1 carries an unusually long 1.9 kb spacer interspersed between two repeat regions and displays a high similarity to pING1-like conjugative plasmids. Finally, we have developed a genetic system for S. islandicus LAL14/1 and created ΔpyrEF and ΔCRISPR_1 mutants using double cross-over and pop-in/pop-out approaches, respectively. Thus, LAL14/1 is a promising model to study virus-host interactions and the CRISPR/Cas defence mechanism in Archaea.
ISSN: 2046-6390

hal-00818894
https://hal.archives-ouvertes.fr/hal-00818894
DOI : 10.1098/rsob.130010
PUBMED : 23594878

Éditeur(s) : HAL CCSD American Association for the Advancement of Science
Résumé : International audience
Most eukaryotic organisms are arthropods. Yet, their diversity in rich terrestrial ecosystems is still unknown. Here we produce tangible estimates of the total species richness of arthropods in a tropical rainforest. Using a comprehensive range of structured protocols, we sampled the phylogenetic breadth of arthropod taxa from the soil to the forest canopy in the San Lorenzo forest, Panama. We collected 6144 arthropod species from 0.48 hectare and extrapolated total species richness to larger areas on the basis of competing models. The whole 6000-hectare forest reserve most likely sustains 25,000 arthropod species. Notably, just 1 hectare of rainforest yields >60% of the arthropod biodiversity held in the wider landscape. Models based on plant diversity fitted the accumulated species richness of both herbivore and nonherbivore taxa exceptionally well. This lends credence to global estimates of arthropod biodiversity developed from plant models.
ISSN: 0036-8075

hal-00817285
https://hal.archives-ouvertes.fr/hal-00817285
https://hal.archives-ouvertes.fr/hal-00817285/document
https://hal.archives-ouvertes.fr/hal-00817285/file/1226727Revisedtext.pdf
DOI : 10.1126/science.1226727

Éditeur(s) : HAL CCSD Springer Verlag
Résumé : International audience
Bone Morphogenetic Protein 9 (BMP9) has been recently found to be the physiological ligand for the activin receptor-like kinase 1 (ALK1), and to be a major circulating vascular quiescence factor. Moreover, a soluble chimeric ALK1 protein (ALK1-Fc) has recently been developed and showed powerful anti-tumor growth and anti-angiogenic effects. However, not much is known concerning BMP9. This prompted us to investigate the human endogenous sources of this cytokine and to further characterize its circulating form(s) and its function. Analysis of BMP9 expression reveals that BMP9 is produced by hepatocytes and intrahepatic biliary epithelial cells. Gel filtration analysis combined with ELISA and biological assays demonstrate that BMP9 circulates in plasma (1) as an unprocessed inactive form that can be further activated by furin a serine endoprotease, and (2) as a mature and fully active form (composed of the mature form associated with its prodomain). Analysis of BMP9 circulating levels during mouse development demonstrates that BMP9 peaks during the first 3 weeks after birth and then decreases to 2 ng/mL in adulthood. We also show that circulating BMP9 physiologically induces a constitutive Smad1/5/8 phosphorylation in endothelial cells. Taken together, our results argue for the role of BMP9 as a hepatocyte-derived factor, circulating in inactive (40%) and active (60%) forms, the latter constantly activating endothelial cells to maintain them in a resting state.
ISSN: 1420-682X

hal-00681969
https://hal.archives-ouvertes.fr/hal-00681969
DOI : 10.1007/s00018-011-0751-1
PUBMED : 21710321

Éditeur(s) : HAL CCSD Elsevier
Résumé : International audience
Liposomal amphotericin B (LAmb) may fail to heal Leishmania infantum visceral leishmaniasis (VL) in the immunodeficient host. There are currently no guidelines on how to treat such patients and efficacy of miltefosine monotherapy seems limited in this indication. We present 2 cases of patients with VL and AIDS for which LAmb had to be interrupted (one because of toxicity, one because of treatment failure) and who were treated effectively with pentamidine followed by miltefosine.
ISSN: 1383-5769

hal-01509592
https://hal.archives-ouvertes.fr/hal-01509592
PUBMED : 27353022

Éditeur(s) : HAL CCSD American Geophysical Union
Résumé : International audience
After the January 12, 2010, Haiti earthquake, we deployed a mainly offshore temporary network of seismologic stations around the damaged area. The distribution of the recorded aftershocks, together with morphotectonic observations and mainshock analysis, allow us to constrain a complex fault pattern in the area. Almost all of the aftershocks have a N‐S compressive mechanism, and not the expected left‐lateral strike‐slip mechanism. A first‐order slip model of the mainshock shows a N264°E north‐dipping plane, with a major left‐lateral component and a strong reverse component. As the aftershock distribution is sub‐parallel and close to the Enriquillo fault, we assume that although the cause of the catastrophe was not a rupture along the Enriquillo fault, this fault had an important role as a mechanical boundary. The azimuth of the focal planes of the aftershocks are parallel to the north‐dipping faults of the Transhaitian Belt, which suggests a triggering of failure on these discontinuities. In the western part, the aftershock distribution reflects the triggering of slip on similar faults, and/or, alternatively, of the south‐dipping faults, such the Trois‐Baies submarine fault. These observations are in agreement with a model of an oblique collision of an indenter of the oceanic crust of the Southern Peninsula and the sedimentary wedge of the Transhaitian Belt: the rupture occurred on a wrench fault at the rheologic boundary on top of the under‐thrusting rigid oceanic block, whereas the aftershocks were the result of the relaxation on the hanging wall along pre‐existing discontinuities in the frontal part of the Transhaitian Belt.
ISSN: 0094-8276

insu-00643489
https://hal-insu.archives-ouvertes.fr/insu-00643489
https://hal-insu.archives-ouvertes.fr/insu-00643489/document
https://hal-insu.archives-ouvertes.fr/insu-00643489/file/GRL-haiti.2011.pdf
DOI : 10.1029/2011GL049799

Éditeur(s) : Oxford Univ Press
Résumé : The impact of the fishing effort exerted by a vessel on a population depends on catchability, which depends on population accessibility and fishing power. The work investigated whether the variation in fishing power could be the result of the technical characteristics of a vessel and/or its gear or whether it is a reflection of inter-vessel differences not accounted for by the technical attributes. These inter-vessel differences could be indicative of a skipper/crew experience effect. To improve understanding of the relationships, landings per unit effort (lpue) from logbooks and technical information on vessels and gears (collected during interviews) were used to identify variables that explained variations in fishing power. The analysis was undertaken by applying a combination of generalized additive models and generalized linear models to data from several European fleets. The study highlights the fact that taking into account information that is not routinely collected, e. g. length of headline, weight of otter boards, or type of groundrope, will significantly improve the modelled relationships between lpue and the variables that measure relative fishing power. The magnitude of the skipper/crew experience effect was weaker than the technical effect of the vessel and/or its gear.
Ices Journal Of Marine Science (1054-3139) (Oxford Univ Press), 2011-11 , Vol. 68 , N. 10 , P. 2252-2263

Droits : 2011 International Council for the Exploration of the Sea. Published by Oxford University Press. All rights reserved.
http://archimer.ifremer.fr/doc/00049/16043/15172.pdf
DOI:10.1093/icesjms/fsr150
http://archimer.ifremer.fr/doc/00049/16043/

Éditeur(s) : HAL CCSD Oxford University Press (OUP)
Résumé : International audience
BACKGROUND: Human immunodeficiency virus (HIV)-infected patients have decreased immune response to vaccines. Few data are available about pandemic flu vaccination in this population. METHODS: We conducted a multicenter, patient-blinded, randomized trial in a cohort of HIV-infected adults. Patients received 2 injections 21 days apart of a AS03(A)-adjuvanted H1N1v vaccine containing 3.75 μg hemagglutinin (HA) or a nonadjuvanted H1N1v vaccine containing 15 μg HA to assess hemagglutination inhibition (HI) response and safety. RESULTS: A total of 309 patients were randomized, and 306 were vaccinated. After the first vaccine dose, HI titers ≥1:40 were observed in 93.4% of the patients in the adjuvanted group (A group) (n = 155) and in 75.5% in the nonadjuvanted group (B group) (n = 151) (P < .001); seroconversion rates were 88.8% and 71.2%, and factor increases in geometric mean titers (GMT) of 21.9 and 15.1, respectively. After 2 injections, 98.6% of patients of the A group and 92.1% of the B group demonstrated HI titers ≥1:40 (P = .018); seroconversion rates were 96.5% and 87.1%, respectively, and factor increases in GMT were 45.5 and 21.2, respectively. The majority of adverse events were mild to moderate in severity; no impact on CD4+ cell count or viral load has been detected. CONCLUSIONS: In HIV-1-infected adults, the AS03(A)-adjuvanted H1N1v vaccine yielded a higher immune response than did the nonadjuvanted one, with no impact on HIV infection.
ISSN: 0022-1899

hal-00875299
https://hal.archives-ouvertes.fr/hal-00875299
DOI : 10.1093/infdis/jir211
PUBMED : 21628666

Éditeur(s) : HAL CCSD
Résumé : International audience
This work presents a synthesis of the present-day knowledge on both emerged and submerged carbonate platforms of the Guadeloupe archipelago. Onshore and offshore data acquired during the KaShallow project are presented. Since the early Zanclean, some isolated platforms developed onto a Jurassic to Tortonian basement which displayed reliefs inherited from a major extensional tectonic episode dated from −8 to −5 Ma. Marie-Galante, Grande Terre and the southern shelf of Grande-Terre show strong similarities with Zanclean to Gelasian red-algal facies upward changing into early Calabrian coral facies. A major erosional unconformity (SB2) was recognized and allows to distinguish two early Calabrian formations (Calcaires à Agaricia Formation and Calcaires à Acropora Formation). The final emersion of these platforms is late Calabrian. The La Désirade platform displays Zanclean to early Piacenzian red-algal facies upward changing into coral facies. This platform emerged as soon as in the late Piacenzian. The island subsided again in relation with later extensional tectonic episode and early Calabrian reef platform then unconformably deposited. The Colombie bank deposits comprise Pliocene basinal deposits overlain by late Calabrian coral reefs. The Flandre bank might have emerged synchronously with La Désirade but was drowned only in the late Pleistocene-Holocene interval. Four extensional tectonic episodes have been identified in the late Miocene-Recent interval. Their effects depend on the location of the platforms within the fore-arc.
Bulletin Société Géologique de France

insu-00816463
https://hal-insu.archives-ouvertes.fr/insu-00816463
DOI : 10.2113/gssgfbull.184.1-2.99

Résumé : Nous avons étudié les phénomènes de polyploïdisation chez l'igname Dioscorea alata qui comprend trois cytotypes diploïde (2n=40), triploïde (2n=60) et tétraploïde (2n=80) en vue d'optimiser les stratégies de production d'hybrides polyploïdes. Chez ce complexe polyploïde l'augmentation de la ploïdie est corrélée avec une augmentation de la vigueur et des rendements plus élevés et plus stables. Dans un premier temps nous avons démontré l'autotétraploïdie des variétés tétraploïdes à l'aide de trois approches différentes: des analyses d'hérédité de marqueurs microsatellites, l'observation de phénomènes de double réduction et l'étude des méioses des cellules mères de pollen. Nous avons ensuite déterminé quels sont les mécanismes les plus probables à l'origine des polyploïdes naturels via l'étude de la transmission de l'hétérozygotie parentale à l'aide de microsatellites et par l'étude des incompatibilités au niveau de l'albumen lors de différents croisements intracytotypes et intercytotypes en utilisant la cytométrie en flux. Les résultats obtenus montrent que les polyploïdes de D. alata seraient apparus via la formation de gamètes non réduits de clones diploïdes. Le pool triploïde se serait édifié et diversifié uniquement par voie femelle, du fait de la non viabilité des croisements intercytotypes et de la stérilité des femelles et mâles triploïdes. Le pool tétraploïde serait apparu par union de deux gamètes non réduits de clones diploïdes (polyploidisation sexuelle bilatérale). Par la suite ce pool aurait été diversifié via des croisements intercy
We studied polyploidisation phenomena in the yam Dioscorea alata that includes three cytotypes -diploid (2n=40), triploid (2n=60) and tetraploid (2n=80) -in order to optimise polyploid hybrid production strategies. In this complex polyploid, the increase in ploidy is correlated with an increase in vigour and higher and more stable yields. We first showed the autotetraploidy of tetraploid varieties using three different approaches: heredity analyses of microsatellite markers, the observation of double reduction phenomena, and the study of meiosis of pollen mother cells. We then determined the mechanisms most likely to be at the origin of natural polyploids through the study of the transmission of parental heterozygoty using microsatellites and the study of incompatibilities at the endosperm level at the time of different intracytotypic and intercytotypic crosses using flow cytometry. The results obtained reveal that the polyploids of D. alata probably appeared through the formation of non-reduced gametes of diploid clones. The triploid pool would then have been constituted and diversified through the female pathway as a result of the non-viability of intercytotypic crosses and the sterility of female and male triploids. The tetraploid pool would have appeared as a result of the union of two non-reduced gametes of diploid clones (bilateral sexual polyploidisation). This pool would then have diversified through intercytotypic crosses with the formation of 2n gamètes through both the female and the male pathway, as well as by intracytotypic crosses within the 4X pool.

http://www.theses.fr/2012AGUY0519/document

Éditeur(s) : HAL CCSD European Respiratory Society
Résumé : International audience
There are conflicting data regarding the magnitude and determinants of chronic obstructive pulmonary disease (COPD) risk in farmers.In a cross-sectional study of 917 nonfarming working controls and 3787 farmers aged 40-75 years, we assessed respiratory symptoms, tobacco exposure, job history (without direct exposure measurement) and lung function. COPD was defined by the Global Initiative for Chronic Obstructive Lung Disease (GOLD) criterion (post-bronchodilator forced expiratory volume in 1 s (FEV1)/forced vital capacity (FVC) extless0.70) and by the Quanjer reference equation (post-bronchodilator FEV1/FVC extlesslower limit of normal (LLN)).The prevalence (95% CI) of COPD according to the GOLD criterion was 5.1% (4.4-5.8%) and 2.9% (1.8-4.0%) in farmers and controls, respectively (p=0.005), and 3.1% (2.5-3.6%) and 1.5% (0.7-2.3%), respectively, for the LLN criterion (p extless0.01). For both COPD criteria after adjustment for age, sex and smoking status, COPD prevalence was similar in controls and crop farmers. Compared to controls, four job categories had a higher prevalence of COPD according to the GOLD criterion, namely, cattle breeders, swine breeders, poultry breeders and breeders of two or more livestock types. Among cattle breeders, only those from Franche-Comté had higher prevalence of COPD according to both GOLD and LLN criteria.The prevalence of COPD in farmers is higher than in nonfarming working controls, and depends on the farming activity, the region and the criterion used to define COPD
ISSN: 0903-1936

hal-01216204
https://hal-univ-rennes1.archives-ouvertes.fr/hal-01216204
DOI : 10.1183/13993003.00153-2015
PUBMED : 26453630