Éditeur(s) : Charles J. Skeet Charles J. Skeet ( London )

Droits : See Google statement.
18601501
http://ufdc.ufl.edu/AA00014504/00001

Éditeur(s) : Charles J. Skeet Charles J. Skeet ( London )

Droits : See Google statement.
18601501
http://ufdc.ufl.edu/AA00014504/00001

Éditeur(s) : HAL CCSD Landes Bioscience
Résumé : International audience
In 2008 we published the first set of guidelines for standardizing research in autophagy. Since then, research on this topic has continued to accelerate, and many new scientists have entered the field. Our knowledge base and relevant new technologies have also been expanding. Accordingly, it is important to update these guidelines for monitoring autophagy in different organisms. Various reviews have described the range of assays that have been used for this purpose. Nevertheless, there continues to be confusion regarding acceptable methods to measure autophagy, especially in multicellular eukaryotes. A key point that needs to be emphasized is that there is a difference between measurements that monitor the numbers or volume of autophagic elements (e.g., autophagosomes or autolysosomes) at any stage of the autophagic process vs. those that measure flux through the autophagy pathway (i.e., the complete process); thus, a block in macroautophagy that results in autophagosome accumulation needs to be differentiated from stimuli that result in increased autophagic activity, defined as increased autophagy induction coupled with increased delivery to, and degradation within, lysosomes (in most higher eukaryotes and some protists such as Dictyostelium) or the vacuole (in plants and fungi). In other words, it is especially important that investigators new to the field understand that the appearance of more autophagosomes does not necessarily equate with more autophagy. In fact, in many cases, autophagosomes accumulate because of a block in trafficking to lysosomes without a concomitant change in autophagosome biogenesis, whereas an increase in autolysosomes may reflect a reduction in degradative activity. Here, we present a set of guidelines for the selection and interpretation of methods for use by investigators who aim to examine macroautophagy and related processes, as well as for reviewers who need to provide realistic and reasonable critiques of papers that are focused on these processes. These guidelines are not meant to be a formulaic set of rules, because the appropriate assays depend in part on the question being asked and the system being used. In addition, we emphasize that no individual assay is guaranteed to be the most appropriate one in every situation, and we strongly recommend the use of multiple assays to monitor autophagy. In these guidelines, we consider these various methods of assessing autophagy and what information can, or cannot, be obtained from them. Finally, by discussing the merits and limits of particular autophagy assays, we hope to encourage technical innovation in the field.
Autophagy

hal-00735751
https://hal.archives-ouvertes.fr/hal-00735751
PUBMED : 22966490

Éditeur(s) : Tahiti Aquaculture 2010, 6-11 décembre 2010

Droits : Tahiti Aquaculture 2010
http://archimer.ifremer.fr/doc/00063/17413/14920.pdf
http://archimer.ifremer.fr/doc/00063/17413/

Éditeur(s) : HAL CCSD Elsevier
Résumé : International audience
ISSN: 0043-1648

hal-01679411
https://hal.univ-antilles.fr/hal-01679411
DOI : 10.1016/j.wear.2004.11.019

Résumé : This report includes a qualitative and descriptive approach of the oyster and mussel industries in the European Community. This report compares the structure of production, the marketsn the professional organisation, competition for the resource, legislation and support systemat the community levell. Technical and statistical datas are summarized to représent the structure of the industry, the trade flows and the main evolutions of the two industries in the EC. A summary of the county reports gives an overview of the present state of the industry in each country and suggest a first evaluation of constraints and prospects. [NOT CONTROLLDED OCR]
Ce rapport comprend une approche descriptive et qualitative du secteur conchylicole traditionnel (moules et huîtres) dans la Communauté Européenne. Il aborde les aspects production, marché, organisation, réglementation, système d'aide dans les différents pays par une analyse comparative des secteurs, complétée par une synthèse chiffrée des données techniques et statistiques. Une vision d'ensemble des flux et de l'évolution des deux filières dans la Communauté est ainsi présentée. Un résumé des rapports nationaux évalue l'organisation, les contraintes et les perspectives du secteur dans chacun des pays. [OCR NON CONTRÔLE]

Droits : 1993 Ifremer
http://archimer.ifremer.fr/doc/1993/rapport-4176.pdf
http://archimer.ifremer.fr/doc/1993/sup-4176.pdf
http://archimer.ifremer.fr/doc/00000/4176/

Éditeur(s) : HAL CCSD Oxford University Press (OUP)
Résumé : International audience
AIM: To describe the main characteristics and the treatment of cryptococcosis in patients with sarcoidosis. DESIGN: Multicenter study including all patients notified at the French National Reference Center for Invasive Mycoses and Antifungals. METHODS: Retrospective chart review. Each case was compared with two controls without opportunistic infections. RESULTS: Eighteen cases of cryptococcosis complicating sarcoidosis were analyzed (13 men and 5 women). With 2749 cases of cryptococcosis registered in France during the inclusion period of this study, sarcoidosis accounted for 0.6% of all the cryptococcosis patients and for 2.9% of the cryptococcosis HIV-seronegative patients. Cryptococcosis and sarcoidosis were diagnosed concomitantly in four cases; while sarcoidosis was previously known in 14/18 patients, including 12 patients (67%) treated with steroids. The median rate of CD4 T cells was 145 per mm(3) (range: 55-1300) and not related to steroid treatment. Thirteen patients had cryptococcal meningitis (72%), three osteoarticular (17%) and four disseminated infections (22%). Sixteen patients (89%) presented a complete response to antifungal therapy. After a mean follow-up of 6 years, no death was attributable to cryptococcosis. Extra-thoracic sarcoidosis and steroids were independent risk factors of cryptococcosis in a logistic regression model adjusted with the sex of the patients. CONCLUSIONS: Cryptococcosis is a significant opportunistic infection during extra-thoracic sarcoidosis, which occurs in one-third of the cases in patients without any treatment; it is not associated to severe CD4 lymphocytopenia and has a good prognosis.
ISSN: 1460-2725

hal-00873410
https://hal.archives-ouvertes.fr/hal-00873410
DOI : 10.1093/qjmed/hct052
PUBMED : 23515400

Éditeur(s) : HAL CCSD Springer Verlag (Germany) Springer Verlag
Résumé : Direct numerical simulations of flow through two millimeter-scale rock samples of limestone and sandstone are performed using three diverse fluid dynamic simulators. The resulting steady-state velocity fields are compared in terms of the associated empirical probability density functions (PDFs) and key statistics of the velocity fields. The pore space geometry of each sample is imaged at 5.06−μm voxel size resolution using X-ray microtomography. The samples offer contrasting characteristics in terms of total connected porosity (about 0.31 for the limestone and 0.07 for the sandstone) and are typical of several applications in hydrogeology and petroleum engineering. The three-dimensional fluid velocity fields within the explicit pore spaces are simulated using ANSYS® FLUENT® ANSYS Inc. (2009), EULAG Prusa et al. (Comput. Fluids 37, 1193–1207 2008), and SSTOKES Sarkar et al. (2002). These computational approaches are highly disperse in terms of algorithmic complexity, differ in terms of their governing equations, the adopted numerical methodologies, the enforcement of internal no-slip boundary conditions at the fluid-solid interface, and the computational mesh structure. As metrics of comparison to probe in a statistical sense the internal similarities/differences across sample populations of velocities obtained through the computational systems, we consider (i) integral quantities, such as the Darcy flux and (ii) main statistical moments of local velocity distributions including local correlations between velocity fields. Comparison of simulation results indicates that mutually consistent estimates of the state of flow are obtained in the analyzed samples of natural pore spaces despite the considerable differences associated with the three computational approaches. We note that in the higher porosity limestone sample, the structures of the velocity fields obtained using ANSYS FLUENT and EULAG are more alike than either compared against the results obtained using SSTOKES. In the low-porosity sample, the structures of the velocity fields obtained by EULAG and SSTOKES are more similar than either is to the fields obtained using ANSYS FLUENT. With respect to macroscopic quantities, ANSYS FLUENT and SSTOKES provide similar results in terms of the average vertical velocity for both of the complex microscale geometries considered, while EULAG tends to render the largest velocity values. The influence of the pore space structure on fluid velocity field characteristics is also discussed.
ISSN: 1420-0597

hal-01174152
https://hal.archives-ouvertes.fr/hal-01174152
DOI : 10.1007/s10596-015-9486-7

Éditeur(s) : HAL CCSD Oxford University Press (OUP)
Résumé : Approximately 4 yr of campaign and continuous Global Positioning System (GPS) measurements across the Dead Sea fault system (DSFS) in Lebanon provide direct measurements of interseismic strain accumulation along a 200-km-long restraining bend in this continental transform fault. Late Cenozoic transpression within this restraining bend has maintained more than 3000 m of topography in the Mount Lebanon and Anti-Lebanon ranges. The GPS velocity field indicates 4-5 mm yr(-1) of relative plate motion is transferred through the restraining bend to the northern continuation of the DSFS in northwestern Syria. Near-field GPS velocities are generally parallel to the major, left-lateral strike-slip faults, suggesting that much of the expected convergence across the restraining bend is likely accommodated by different structures beyond the aperture of the GPS network (e.g. offshore Lebanon and, possibly, the Palmyride fold belt in SW Syria). Hence, these geodetic results suggest a partitioning of crustal deformation involving strike-slip displacements in the interior of the restraining bend, and crustal shortening in the outer part of the restraining bend. Within the uncertainties, the GPS-based rates of fault slip compare well with Holocene-averaged estimates of slip along the two principal strike-slip faults: the Yammouneh and Serghaya faults. Of these two faults, more slip occurs on the Yammouneh fault, which constitutes the primary plate boundary structure between the Arabia and Sinai plates. Hence, the Yammouneh fault is the structural linkage that transfers slip to the northern part of the transform in northwestern Syria. From the perspective of the regional earthquake hazard, the Yammouneh fault is presently locked and accumulating interseismic strain.
ISSN: 0956-540X

hal-00406620
https://hal.archives-ouvertes.fr/hal-00406620
DOI : 10.1111/j.1365-246X.2006.03328.x

Éditeur(s) : HAL CCSD Nature Publishing Group
Résumé : International audience
Collisional mountain belts grow as a consequence of continental plate convergence and eventuallydisappear under the combined effects of gravitational collapse and erosion. Using a decade ofGPS data, we show that the western Alps are currently characterized by zero horizontal velocityboundary conditions, offering the opportunity to investigate orogen evolution at the time ofcessation of plate convergence. We find no significant horizontal motion within the belt, but GPS andlevelling measurements independently show a regional pattern of uplift reaching ~2.5 mm/yr in thenorthwestern Alps. Unless a low viscosity crustal root under the northwestern Alps locally enhancesthe vertical response to surface unloading, the summed effects of isostatic responses to erosion andglaciation explain at most 60% of the observed uplift rates. Rock-uplift rates corrected from transientglacial isostatic adjustment contributions likely exceed erosion rates in the northwestern Alps. In theabsence of active convergence, the observed surface uplift must result from deep-seated processes.
ISSN: 2045-2322

hal-01357450
https://hal.archives-ouvertes.fr/hal-01357450
https://hal.archives-ouvertes.fr/hal-01357450/document
https://hal.archives-ouvertes.fr/hal-01357450/file/noquet2016.pdf
DOI : 10.1038/srep28404

Éditeur(s) : HAL CCSD Elsevier
Résumé : This paper is the first presentation of a project called GHYRAF (Gravity and Hydrology in Africa) devoted to the detailed comparison between models and multidisciplinary observations (ground and satellite gravity, geodesy, hydrology, meteorology) of the variations of water storage in Africa from the Sahara and part to the monsoon equatorial part. We describe the various actions planned in this project. We first detail the actions planned in gravimetry which consist in two main surface gravity experiments: on the one hand the periodic repetition of absolute gravity measurements along a north-south monsoonal gradient of rainfall in West Africa, going from Tamanrasset (20 mm/year) in southern Algeria to Djougou (1200 mm/year) in central Benin; on the other hand the continuous measurements at Djougou (Benin) with a superconducting gravimeter to monitor with a higher sampling rate the gravity changes related to an extreme hydrological cycle. Another section describes the actions planned in GPS which will maintain and develop the present-day existing network in West Africa. The third type of actions deals with hydrology and we review the three sites that will be investigated in this joint hydrogeophysics project namely Wankama (near Niamey) and Bagara (near Diffa) in the Niger Sahelian zone and Nalohou (near Djougou) in the Benin monsoon zone. We also address the question of the ground truth of satellite-derived missions: in this context the GHYRAF project will lead to test the hydrology models by comparison both with in situ and satellite data such as GRACE, as well as to an important increase of our knowledge of the seasonal water cycle in Africa. We finally present preliminary results in GPS based on the analysis of the vertical motion of the Djougou site. The resulting absolute gravity changes related to the 2008 monsoon are finally given.
ISSN: 0264-3707

hal-00475660
https://hal.archives-ouvertes.fr/hal-00475660
DOI : 10.1016/j.jog.2009.09.014

Éditeur(s) : HAL CCSD
Résumé : International audience
EGU General Assembly 2014
Vienne, Austria

insu-01113701
https://hal-insu.archives-ouvertes.fr/insu-01113701

Éditeur(s) : HAL CCSD Elsevier
Résumé : International audience
This study analyzes the active tectonics within the northwestern and southeastern extensions of the Pambak-Sevan-Syunik fault (PSSF), a major right-lateral strike-slip fault cutting through Armenia. Quantifying the deformations in terms of geometry, kinematics, slip rates and earthquake activity, using cosmogenic 3He, OSL/IRSL and radiocarbon dating techniques, reveal different behaviors between the two regions. Within the northwestern extension, in the region of Amasia, the PSSF bends to the west and splits into two main WNW–ESE trending reverse faults defining a compressional pop-up structure. We estimate an uplift rate and a shortening rate of 0.5 ± 0.1 mm/y and 1.4 ± 0.6 mm/y, respectively. This suggests that most of the ∼2 mm/y right lateral movement of the PSSF seems to be absorbed within the Amasia pop-structure. Within the southeastern extension, the PSSF shows signs of dying out within the Tsghuk Volcano region at the southernmost tip of the Syunik graben. There, the tectonic activity is characterized by a very slow NS trending normal faulting associated with a slight right-lateral movement. Slip rates analyses (i.e. vertical slip rate, EW stretching rate at 90° to the fault, and right-lateral slip rate of ∼0.2 mm/y, ∼0.1 mm/y and ∼0.05 mm/y, respectively) lead to the conclusion that the right lateral movement observed further north along the PSSF is mainly transferred within other active faults further west within the Karabagh (Hagari fault or other structures further northwestwards). Comparing our slip rates with those estimated from GPS data suggests that most of the deformation is localized and seismic, at least within the Tsghuk region. The geometrical and kinematic pattern observed within the two terminations of the PSSF suggests that the fault and its surrounding crustal blocks are presently rotating anticlockwise, as also observed within the GPS velocity field. This is consistent with the recent kinematic models proposed for the Caucasus-Kura-South Caspian region and brings a new insight into the present geodynamics of Armenia.
ISSN: 1040-6182

hal-01307342
https://hal.archives-ouvertes.fr/hal-01307342
DOI : 10.1016/j.quaint.2015.05.021

Éditeur(s) : HAL CCSD Springer Verlag
Résumé : GPS velocities and seismicity across the Betic-Rif Arc structural domains (Morocco and Iberia) provide a basis to evaluate present-day seismotectonic processes between different deformation belts. The results show asymmetric movements in the complex Alboran system accommodating the convergence between the African (Nubian) and Eurasian plates. While the Betic Mountains are attached to Iberia, moving toward the southeast with respect to Africa, the Rif is divided into three blocks with distinct displacements relative to Nubia: (1) the Tangier block moving southeastward, (2) the Central Rif block moving SSW, and (3) the Oriental Rif block undergoing clockwise rotation. GPS-derived motions decrease in rate from the Rif nappes complex to the foreland.
ISSN: 0010-7999

hal-00411144
https://hal.archives-ouvertes.fr/hal-00411144
DOI : 10.1016/j.crte.2008.02.003

Éditeur(s) : HAL CCSD Ferrata Storti Foundation
Résumé : International audience
Lionnet et al. recently reported a high prevalence of retinopathy (RET) and otologic disorders (OTD) in patients with sickle cell-hemoglobin C disease (SC), while a significant number of patients had renal diseases (mainly glomerulopathy; GLO) and osteonecrosis (OST). The pathophysiological processes of these complications in SC are not well defined, although blood hyperviscosity has been suspected, but never tested to the best of our knowledge, as responsible for several chronic complications in SC disease1,2. The aim of this study was to analyze the associations between hematological and hemorheological parameters and chronic complications in adult SC patients.
ISSN: 0390-6078

inserm-01081709
http://www.hal.inserm.fr/inserm-01081709
http://www.hal.inserm.fr/inserm-01081709/document
http://www.hal.inserm.fr/inserm-01081709/file/2014%2C%20Lemonne%20-%20Impaired%20blood%20rheology%20plays%20a%20role%20in%20the%20chronic%20disorders%20associated%20with%20sickle%20cell-hemoglobin%20C%20disease.pdf
PUBMED : 24633868

Éditeur(s) : HAL CCSD
2012 AGU Fall Meeting

hal-00771546
https://hal-mines-paristech.archives-ouvertes.fr/hal-00771546

Éditeur(s) : HAL CCSD Elsevier
Résumé : International audience
We use velocities from 65 continuous stations and 31 survey-mode GPS sites as well as kinematic modeling to investigate present day deformation along the Africa-Iberia plate boundary zone in the western Mediterranean region. The GPS velocity field shows southwestward motion of the central part of the Rif Mountains in northern Morocco with respect to Africa varying between 3.5 and 4.0 mm/yr, consistent with prior published results. Stations in the southwestern part of the Betic Mountains of southern Spain move west-southwest with respect to Eurasia (similar to 2-3 mm/yr). The western component of Betics motion is consistent with partial transfer of Nubia-Eurasia plate motion into the southern Betics. The southward component of Betics motion with respect to Iberia is kinematically consistent with south to southwest motion of the Rif Mountains with respect to Africa. We use block modeling, constrained by mapped surface faults and seismicity to estimate the geometry and rates of strain accumulation on plate boundary structures. Our preferred plate boundary geometry includes one block between Iberia and Africa including the SW Betics, Alboran Sea, and central Rif. This geometry provides a good fit to the observed motions, suggesting a wide transpressive boundary in the westernmost Mediterranean, with deformation mainly accommodated by the Gloria-Azores fault system to the West and the Rif-Tell lineament to the East. Block boundaries encompass aspects of earlier interpretations suggesting three main deformation styles: (i) extension along the NE-SW trending Trans-Alboran shear zone, (ii) dextral strike-slip in the Betics corresponding to a well defined E-W seismic lineament, and (iii) right lateral strike-slip motion extending West to the Azores and right-lateral motion with compression extending East along the Algerian Tell. We interpret differential motion in the Rif-Alboran-Betic system to be driven both by surface processes related the Africa-Eurasia oblique convergence and sub-crustal dynamic processes associated with the long history of subduction of the Neotethys ocean lithosphere. The dextral slip identified in the Betic Mountains in Southern Spain may be related to the offshore fault that produced the Great 1755 Lisbon Earthquake, and as such may represent a significant seismic hazard for the West Mediterranean region.
ISSN: 0012-821X

hal-00618337
https://hal.archives-ouvertes.fr/hal-00618337
DOI : 10.1016/j.epsl.2011.05.048

Éditeur(s) : HAL CCSD Elsevier Masson
Résumé : International audience
Cancer is the main complication of transplantation surgery. The literature concerning renal transplant recipients among the Afro-Caribbean population is scant. The aim of this study was to determine the incidence of cancer in these patients, with the secondary objective being to identify predisposing factors for cancer. This was an epidemiological and retrospective study that included all Guadeloupians of phototype V-VI undergoing renal transplantation from 01/01/2004 to 31/12/2011. Skin cancer screening was performed before transplantation and during an annual dermatological consultation following transplantation. Screening for non-cutaneous cancers was guided by clinical symptoms or by the results of the screening examinations recommended in the current guidelines. At the study time-point (31/12/2011), all patients were examined by a dermatologist. One hundred and two patients were included : 42 women and 60 men (mean age: 52.1±11.6 years at transplantation). Eight cancers were diagnosed. The cumulative incidence of cancer was 7.8% at 3 years. Three factors were associated with more rapid onset of cancer: personal history or familial history of cancer, and genital lesion induced by HPV. Our results suggest a low incidence of cancer in Afro-Caribbean renal transplant patients. Personal or family history of cancer and HPV-induced genital lesions would appear to accelerate the onset of cancer in this population.
ISSN: 0151-9638

hal-01260468
https://hal.archives-ouvertes.fr/hal-01260468
https://hal.archives-ouvertes.fr/hal-01260468/document
https://hal.archives-ouvertes.fr/hal-01260468/file/2015%2C%20Ameline.pdf
https://hal.archives-ouvertes.fr/hal-01260468/file/14%20148%20Fig%201%20ap.pdf

Éditeur(s) : HAL CCSD Public Library of Science
Résumé : International audience
A decreased global autonomic nervous system (ANS) activity and increased sympathetic activation in patients with sickle cell anemia (SCA) seem to worsen the clinical severity and could play a role in the pathophysiology of the disease, notably by triggering vaso-occlusive crises. Because exercise challenges the ANS activity in the general population, we sought to determine whether a short (,15 min) and progressive moderate exercise session conducted until the first ventilatory threshold had an effect on the ANS activity of a group of SCA patients and a group of healthy individuals (CONT group). Temporal and spectral analyses of the nocturnal heart rate variability were performed before and on the 3 nights following the exercise session. Standard deviation of all normal RR intervals (SDNN), total power, low frequencies (LF) and high frequencies powers (HF) were lower but LF/HF was higher in SCA patients than in the CONT group. Moderate exercise did not modify ANS activity in both groups. In addition, no adverse clinical events occurred during the entire protocol. These results imply that this kind of short and moderate exercise is not detrimental for SCA patients.
ISSN: 1932-6203

hal-01136725
https://hal.univ-antilles.fr/hal-01136725
https://hal.univ-antilles.fr/hal-01136725/document
https://hal.univ-antilles.fr/hal-01136725/file/Hedreville%20et%20al.%20-%202014%20-%20Acute%20moderate%20exercise%20does%20not%20further%20alter%20the%20autonomic%20nervous%20system%20activity%20in%20patients%20with%20sick%282%29.pdf
DOI : 10.1371/journal.pone.0095563

Éditeur(s) : HAL CCSD Wiley
Résumé : International audience
MR and MDHD contributed equally to this work. Summary Painful vaso-occlusive crisis, a hallmark of sickle cell anaemia, results from complex, incompletely understood mechanisms. Red blood cell (RBC) damage caused by continuous endogenous and exogenous oxidative stress may precipitate the occurrence of vaso-occlusive crises. In order to gain insight into the relevance of oxidative stress in vaso-occlusive crisis occurrence , we prospectively compared the expression levels of various oxidative markers in 32 adults with sickle cell anaemia during vaso-occlusive crisis and steady-state conditions. Compared to steady-state condition, plasma levels of free haem, advanced oxidation protein products and myeloperoxi-dase, RBC caspase-3 activity, as well as the concentrations of total, neutrophil-and RBC-derived microparticles were increased during vaso-occlusive crises, whereas the reduced glutathione content was decreased in RBCs. In addition, natural anti-band 3 autoantibodies levels decreased during crisis and were negatively correlated with the rise in plasma advanced oxidation protein products and RBC caspase-3 activity. These data showed an exacerbation of the oxidative stress during vaso-occlusive crises in sickle cell anaemia patients and strongly suggest that the higher concentration of harmful circulating RBC-derived microparticles and the reduced anti-band 3 autoantibodies levels may be both related to the recruitment of oxidized band 3 into membrane aggregates.
ISSN: 0007-1048

inserm-01476067
http://www.hal.inserm.fr/inserm-01476067
http://www.hal.inserm.fr/inserm-01476067/document
http://www.hal.inserm.fr/inserm-01476067/file/2016%2C%20Hierso%20-%20Exacerbation%20of%20oxidative%20stress%20during%20sickle%20vaso-occlusive%20crisis.pdf
DOI : 10.1111/bjh.14476