Éditeur(s) : HAL CCSD Editions Paralia CFL
Résumé : SUBDUNE v0.6 is a very preliminary version of an exploratory model designed tocompute the sea level to the shoreline, combining the effects of various physics, from run-up to tides. It aims at providing an easy-to-use and quick way to estimate water elevation to the beach, assuming morphology is known. Combined with accurate beachdata, such as LIDAR, this engineering tool may provide with end-users an efficient way to quantify submersion.
ISSN: 1760-8716

hal-00854170
https://hal.archives-ouvertes.fr/hal-00854170
DOI : 10.5150/jngcgc.2012.022-B

Éditeur(s) : HAL CCSD Elsevier
Résumé : International audience
The estrogen receptor (ER) is a transcription factor that binds under 17-b-estradiol (E2) stimulation as homodimer to a short DNA consensus sequence named estrogen response element (ERE). The ER/ERE interaction has been assessed by several research groups through different methodologies notably by surface plasmon resonance (SPR) techniques. The biochemical parameters and conditions (solvent, ER concentration, salt, time and temperature) used to prepare samples before analysis were very different from one study to another. But no studies have aimed to compare the effect of these modifications on ER/ERE interaction. Therefore the main objective of the present paper was to assess the influence of biochemical parameters onto the ER/ERE interaction with the final aim to improve the comprehension of this interaction. Our results highlighted that parameters like solvent, ER concentration, salt and surfactant concentration, temperature and time deeply modify ER/ERE interaction. Nevertheless, the dimer formation under E2 stimulation occurred with all tested conditions. Altogether, incubation parameters of ER with E2, deeply modify its binding level onto ERE. These data constitute an important key point to consider for the improvement of ER/ERE detection method depending upon the aim of the study (interaction measurement, environmental detection, development of new technologies or devices).
ISSN: 0003-9861

hal-00936061
https://hal.archives-ouvertes.fr/hal-00936061
https://hal.archives-ouvertes.fr/hal-00936061/document
https://hal.archives-ouvertes.fr/hal-00936061/file/Impactsur biochemical_design_on_estrogen_receptor_estrogen_response_element_interaction_by_surface_plasmon_resonance_technology-accepted.pdf
DOI : 10.1016/j.abb.2013.11.006

Éditeur(s) : HAL CCSD American Physical Society
Résumé : International audience
It is getting increasingly evident that physical properties such as elastoviscoplastic properties of living materials are quite important for the process of tissue development, including regulation of genetic pathways. Measuring such properties in vivo is a complicated and challenging task. In this paper, we present an instrument, a scanning air puff tonometer, which is able to map point by point the viscoelastic properties of flat or gently curved soft materials. This instrument is an improved version of the air puff tonometer used by optometrists, with important modifications. The instrument allows one to obtain a direct insight into gradients of material properties in vivo. The instrument capabilities are demonstrated on substances with known elastoviscoplastic properties and several biological objects. On the basis of the results obtained, the role of the gradients of elastoviscoplastic properties is outlined for the process of angiogenesis, limb development, bacterial colonies expansion, etc. which is important for bridging the gaps in the theory of the tissue development and highlighting new possibilities for tissue engineering, based on a clarification of the role of physical features in developing biological material.
ISSN: 1539-3755

hal-00666187
https://hal.archives-ouvertes.fr/hal-00666187
https://hal.archives-ouvertes.fr/hal-00666187/document
https://hal.archives-ouvertes.fr/hal-00666187/file/FLEURY_PRE_2010.pdf
DOI : 10.1103/PhysRevE.81.021920

Éditeur(s) : HAL CCSD Wiley
Résumé : International audience
Well studied in mammals, amelogenesis is less known at the molecular level in reptiles and amphibians. In the course of extensive studies of enamel matrix protein (EMP) evolution in tetrapods, we look for correlation between changes in protein sequences and temporospatial protein gene expression during amelogenesis, using an evo-devo approach. Our target is the major EMP, amelogenin (AMEL) that plays a crucial role in enamel structure. We focused here our attention to an amphibian, the salamander Pleurodeles waltl. RNAs were extracted from the lower jaws of a juvenile P. waltl and the complete AMEL sequence was obtained using PCR and RACE PCR. The alignment of P. waltl AMEL with other tetrapodan (frogs, reptiles and mammals) sequences revealed residue conservation in the N- and C-terminal regions, and a highly variable central region. Using sense and anti-sense probes synthetized from the P. waltl AMEL sequence, we performed in situ hybridization on sections during amelogenesis in larvae, juveniles, and adults. We demonstrated that (i) AMEL expression was always found to be restricted to ameloblasts, (ii) the expression pattern was conserved through ontogeny, even in larvae where enameloid is present in addition to enamel, and (iii) the processes are similar to those described in lizards and mammals. These findings indicate that high variations in the central region of AMEL have not modified its temporospatial expression during amelogenesis for 360 million years of tetrapod evolution. J. Exp. Zool. (Mol. Dev. Evol.) 320B:200209, 2013.
ISSN: 1552-5007

hal-01545483
https://hal.archives-ouvertes.fr/hal-01545483
DOI : 10.1002/jez.b.22494

Éditeur(s) : HAL CCSD Elsevier
Résumé : International audience
Dietary exposure to pesticide residues may present a risk to public health, especially for sensitive populations such as pregnant women. To characterize this risk, this study assessed chronic dietary exposure to pesticide residues based on the French ELFE cohort. A self-administered food frequency questionnaire (FFQ) about the last three months of pregnancy filled in by pregnant women in 2011 was used in combination with occurrence data from French Total Diet Studies completed by the results of national monitoring programs on pesticide residues in food. The dietary intake of pesticides (μg/kg of body weight/day) was estimated for 14,099 pregnant women with a complete FFQ, for 317 substances under two occurrence scenarios to handle left-censored data: a lower-bound scenario (LB), where undetected results were set to zero, and an upper-bound scenario (UB), where undetected results were set to the detection limit if the substance was expected to be found in food and zero if it was not. The risk was assessed for 284 substances with a toxicological reference value (TRV) and a good coverage level of the diet potentially contributing to pesticide intake. The cumulative risk was also assessed for seven effects on nervous and thyroid systems using the hazard index and the Cumulative Assessment Groups defined by EFSA. Substances with the highest exposure levels under the LB scenario were, in decreasing order, imazalil, piperonyl butoxide, chlorpropham, thiabendazole, iprodione and propargite. Under the LB scenario, only for lindane did women have a statistically significant probability of exceeding the TRV (2.4%). Under the UB scenario, risk could not be excluded for nine other substances. A better management of left-censored data and more sensitive analyses of the main food contributors might help to refine the UB exposure and risk assessments. A statistically significant cumulative risk was found for neurochemical effects related to high intake levels of three organophosphate insecticides (chlorpyrifos, pirimiphos-methyl and dimethoate) mainly detected in fruits and cereals
ISSN: 0160-4120

hal-01319892
https://hal-univ-rennes1.archives-ouvertes.fr/hal-01319892
DOI : 10.1016/j.envint.2016.04.007
PUBMED : 27187793

Éditeur(s) : HAL CCSD
Résumé : A standard approach for large scale image classification involves high dimensional features and Stochastic Gradient Descent algorithm (SGD) for the minimization of classical Hinge Loss in the primal space. Although complexity of Stochastic Gradient Descent is linear with the number of samples these method suffers from slow convergence. In order to cope with this issue, we propose here a Stochastic Low-Rank Newton Descent SLND for minimization of any calibrated loss in the primal space. SLND approximates the inverse Hessian by the best low-rank approximation according to squared Frobenius norm. We provide core optimization for fast convergence. Theoretically speaking, we show explicit convergence rates of the algorithm using these calibrated losses, which in addition provide working sets of parameters for experiments. Experiments are provided on the SUN, Caltech256 and ImageNet databases, with simple, uniform and efficient ways to tune remaining SLND parameters. On each of these databases, SLND challenges the accuracy of SGD with a speed of convergence faster by order of magnitude.
https://hal.archives-ouvertes.fr/hal-00825414

hal-00825414
https://hal.archives-ouvertes.fr/hal-00825414
https://hal.archives-ouvertes.fr/hal-00825414/document
https://hal.archives-ouvertes.fr/hal-00825414/file/TechnicalReport.pdf

Éditeur(s) : HAL CCSD
Résumé : International audience
Understand the dynamics of the fresh-salt water interface in aquifers is a key issue to comprehend mixing processand to quantity the discharge of nutrients in to coastal areas. In order to go beyond the current knowledge inthis issue an experimental site has been set up at the alluvial aquifer Riera Argentona (Barcelona – Spain). Thesite comprises 16 shallow piezometers installed between 30 and 90 m from the seashore, with depths rangingbetween 15 and 25 meters. The seawater interface is being monitored using several techniques, the combinationof which will help us to understand the spatial and temporal behaviour of the mixing zone and the geochemicalprocesses occurring there. Specially the deepest piezometers are equipped with electrodes in order to performcross-hole electrical resistivity tomography (CHERT). In addition, all piezometers are also equipped with FiberOptic cable to perform distributed temperature measurements. Two single steel armoured fibre optic cable lines ofaround 600m length were installed in all boreholes. The objective is to use the cable both as passive and activetemperature sensor. The first is being done for the continuous monitoring of temperature whereas; the secondprovides a higher temperature resolution used to monitor field experiments.Periodic CHERT measurements are carried out between the piezometer equipped with electrodes, resultingin parallel and perpendicular vertical cross sections of the site resistivity. The position of the fresh-salt waterinterface can be identified due to the resistivity contrast between the saline and fresh water. Preliminary resultsof periodic distributed temperature measurements will be also be used to monitor the position of the mixing zonethanks to the contrast and seasonal temperature changes. Periodic down-hole EC profiles will be used to validatethe method.AcknowledgementsThis work was funded by the projects CGL2013-48869-C2-1 y CGL2013-48869-C2-2-R of the Spanish Government.We would like to thank SIMMAR (Serveis Integrals de Manteniment del Maresme) and the ConsellComarcal del Maresme in the construction of the research site
European Geosciences Union General Assembly 2016
Vienne, Austria

insu-01308757
https://hal-insu.archives-ouvertes.fr/insu-01308757

Éditeur(s) : HAL CCSD American Association for the Advancement of Science
Résumé : International audience
Sediments cored along the southwestern Iberian margin during Integrated Ocean Drilling Program Expedition 339 provide constraints on Mediterranean Outflow Water (MOW) circulation patterns from the Pliocene epoch to the present day. After the Strait of Gibraltar opened (5.33 million years ago), a limited volume of MOW entered the Atlantic. Depositional hiatuses indicate erosion by bottom currents related to higher volumes of MOW circulating into the North Atlantic, beginning in the late Pliocene. The hiatuses coincide with regional tectonic events and changes in global thermohaline circulation (THC). This suggests that MOW influenced Atlantic Meridional Overturning Circulation (AMOC), THC, and climatic shifts by contributing a component of warm, saline water to northern latitudes while in turn being influenced by plate tectonics.
ISSN: 0036-8075

hal-01054316
https://hal.archives-ouvertes.fr/hal-01054316
DOI : 10.1126/science.1251306

Éditeur(s) : HAL CCSD Geological Society of London
Résumé : The Zagros Simply Folded Belt (ZSFB) is an active fold-and-thrust belt resulting from the still continuing continental collision between the Arabian plate and the Iranian plate, which probably started in the Oligocene. The present-day shortening (N25°) is well documented by focal mechanisms of earthquakes and global positioning system (GPS) surveys. We propose in this study a comparison of published palaeostress markers, including magnetic fabric, brittle deformation and calcite twinning data. In addition, we describe the magnetic fabric from Palaeocene carbonates (10 sites) and Mio-Pliocene clastic deposits (15 sites). The magnetic fabrics are intermediate, with magnetic foliation parallel to the bedding, and a magnetic lineation mostly at right angles to the shortening direction. This suggests that the magnetic fabric retains the record of an early layer-parallel shortening (LPS) that occurred prior to folding. The record of LPS allows the identification of originally oblique folds such as the Mand Fold, which have developed in front of the Kazerun Fault. The shape parameter of the magnetic fabric indicates a weak strain compatible with the development of detachment folds in the ZSFB. The palaeostress datasets, covering the Palaeocene to Pleistocene time interval, support several folding episodes accompanied by a counter-clockwise rotation of the stress field direction. The Palaeocene carbonates in the ZSFB record a N47 LPS during early to middle Miocene detachment folding in the High Zagros Belt (HZB). The Mio-Pliocene clastic deposits recorded a N38 LPS prior to and during detachment folding within the ZSFB at the end of the Miocene-Pliocene. Similarly, fault slip and calcite twin data from the ZSFB also support a counter-clockwise rotation from NE to N20 between the pre-folding stage and the late rejuvenation of folds. This counter-clockwise trend of palaeostress data agrees with fault slip data from the HZB. During the late stage of folding in the ZSFB, the Plio-Quaternary palaeostress trends are consistently parallel to the present-day shortening direction.
Tectonic and stratigraphic evolution of Zagros and Makran during the Mesozoic-Cenozoic

hal-00545036
https://hal.archives-ouvertes.fr/hal-00545036
DOI : 10.1144/SP330.6

Éditeur(s) : HAL CCSD Wiley
Résumé : The evolutionary potential of long-lived species, such as forest trees, is fundamental for their local persistence under climate change (CC). Genome–environment association (GEA) analyses reveal if species in heterogeneous environments at the regional scale are under differential selection resulting in populations with potential preadaptation to CC within this area. In 79 natural [i]Fagus sylvatica[/i] populations, neutral genetic patterns were characterized using 12 simple sequence repeat (SSR) markers, and genomic variation (144 single nucleotide polymorphisms (SNPs) out of 52 candidate genes) was related to 87 environmental predictors in the latent factor mixed model, logistic regressions and isolation by distance/environmental (IBD/IBE) tests. SSR diversity revealed relatedness at up to 150m intertree distance but an absence of large-scale spatial genetic structure and IBE. In the GEA analyses, 16 SNPs in 10 genes responded to one or several environmental predictors and IBE, corrected for IBD, was confirmed. The GEA often reflected the proposed gene functions, including indications for adaptation to water availability and temperature. Genomic divergence and the lack of large-scale neutral genetic patterns suggest that gene flow allows the spread of advantageous alleles in adaptive genes. Thereby, adaptation processes are likely to take place in species occurring in heterogeneous environments, which might reduce their regional extinction risk under CC
ISSN: 0028-646X

hal-01309135
https://hal.archives-ouvertes.fr/hal-01309135
PRODINRA : 343710
DOI : 10.1111/nph.13809

Éditeur(s) : HAL CCSD Geological Society of America
Résumé : International audience
In this study, a novel application of a statistical approach is utilized for analysis of downhole logging data from Miocene-aged siliciclastic shelf sediments on the New Jersey Margin (eastern USA). A multivariate iterative nonhierarchical cluster analysis (INCA) of spectral gamma-ray logs from Integrated Ocean Drilling Program (IODP) Expedition 313 enables lithology within this siliciclastic succession to be inferred and, through comparison with the 1311 m of recovered core, a continuous assessment of depositional sequences is constructed. Signifi cant changes in INCA clusters corroborate most key stratigraphic surfaces interpreted from the core, and this result has particular value for surface recognition in intervals of poor core recovery. This analysis contributes to the evaluation of sequence stratigraphic models of large-scale clinoform complexes that predict depositional environments, sediment composition, and stratal geometries in response to sea-level changes. The novel approach of combining statistical analysis with detailed lithostratigraphic and seismic refl ection data sets will be of interest to any scientists working with downhole logs, especially spectral gamma-ray data, and also provides a reference for the strengths and weaknesses of multi component analysis applied to continental margin lithofacies. The method presented here is appropriate for evaluating successions elsewhere and also has value for hydrocarbon exploration where sequence stratigraphy is a fundamental tool.
EISSN: 1553-040X

insu-00858238
https://hal-insu.archives-ouvertes.fr/insu-00858238
DOI : 10.1130/GES00913.1

Éditeur(s) : HAL CCSD
Résumé : National audience
Un ensemble de données sur l’environnement géologique despéridotites de la Zone Nord-Pyrénéenne a été obtenu dans lecadre des programmes ANR Pyramid et RGF-BRGM consacrésà l’évolution géodynamique des Pyrénées. Ces données représententdes contraintes indispensables pour l’étude des processus del’exhumation du manteau, de l’amincissement de la croûte continentaleet de sa disparition complète lors de l’ouverture desbassins transtensifs albo-cénomaniens. On y trouve : (1) de nouveauxlevés géologiques de détail autour des lherzolites de Lherz-Freychinède-Vicdessos-Bestiac-Prades et d’Urdach-Les Pernes et(2) des analyses multi-méthodes des métasédiments de la couvertureprérift et du socle paléozoïque adhérent au manteau :composition des assemblages du métamorphisme HT, températuresmaximales par spectrométrie RAMAN sur la m.o., fabriquede la calcite par EBSD, inclusions fluides et isotopes stables dansles marbres et veines associées, datations U-Th-Pb in situ. Cesdonnées permettent d’estimer les conditions thermiques lors de laremontée du manteau et de sa mise à l’affleurement. Nous montronsune variabilité remarquable de l’Ouest vers l’Est.A l’Ouest, le manteau est exhumé à l’Albien sup. - Cénomanieninf. en perçant une couverture mésozoïque prérift qui n’a pas étéchauffée à plus de 250C. La croûte continentale supérieure y estréduite en lames ductiles extrêmement amincies puis cataclaséeset remaniées sur le fond ultrabasique avec des fragments de manteauet de la couverture prérift, durant le dépôt du flysch noir. Latempérature augmente au cours du Cénomanien (480C) dans leszones où le manteau n’a pas été mis à l’affleurement. Le Triassupérieur se transforme profondément (talc-chlorites) et perd sacomposante évaporitique. La couverture prérift décollée joue unrôle majeur comme couvercle isotherme, suivi en cela par les flyschsalbiens puis cénomaniens.A l’Est, le manteau perce à travers une couverture chauffée localementà plus de 600C. Le Keuper y est réduit à quelquesdécimètres de grès et brèches riches en saphirine. La croûtesupérieure a été presque totalement éliminée tectoniquement, maisdes granulites sont remaniées à côté des péridotites et des marbresdans les brèches recouvrant le manteau exhumé.Ces données de terrain représentent les contraintes de base pourconstruire les modèles d’amincissement crustal. La variabilitémise en évidence (gradient thermique et présence ou absence decroûte profonde remaniée) ne permet pas de proposer un modèled’amincissement simple et unique. Cependant, toutes ces donnéesindiquent que la croûte moyenne à supérieure se comporteductilement sous les écrans thermiques de la couverture prériftet synrift, mobiles sur le Keuper. Un modèle de marge passivedistale faisant appel à des blocs continentaux rigides de type ”extensional allochthons ” ne peut s’appliquer ici. Sur la base deces données géologiques, nous mettons donc en avant un nouveaumodèle de type marge distale chaude plus ou moins transtensive.
RST 2016, livre des résumés
Caen, France

insu-01388463
https://hal-insu.archives-ouvertes.fr/insu-01388463

Éditeur(s) : HAL CCSD Paris : L'Harmattan
Résumé : International audience
En s'appuyant sur des observations ethnographiques longitudinales et des entretiens compréhensifs, cet article propose une description du fonctionnement socioculturel et une analyse des pratiques éducatives familiales des deux groupes considérés, ainsi qu'un regard sur l'évolution de ces facteurs dans le contexte mouvant du monde actuel. Une connaissance des pratiques éducatives de ces populations pourrait fournir aux enseignants et spécialistes des pistes de réflexion pour une meilleure prise en compte des besoins éducatifs des populations amérindiennes vivant sur les sites isolés des fleuves amazoniens et une adaptation de l'enseignement scolaire aux spécificités culturelles.
ISSN: 1967-5798

hal-01058307
https://hal.archives-ouvertes.fr/hal-01058307
https://hal.archives-ouvertes.fr/hal-01058307/document
https://hal.archives-ouvertes.fr/hal-01058307/file/Ailincai_R._Jund_S._et_Ali_M._2012_._Comparaison_des_A_cosystA_mes_A_ducatifs_chez_deux_groupes_d_AmA_rindiens_WayA_pi_et_Wayana.pdf

Éditeur(s) : HAL CCSD Integrated Ocean Drilling Program
Résumé : This contribution is a compilation of electron microprobe analyses of primary minerals in mafic and ultramafic oceanic crustal rocks from Atlantis Massif. The samples were recovered during Integrated Ocean Drilling Program Expedition 304/305, which was designed to investigate the formation and evolution of oceanic core complexes. The analyses were performed at five different institutions, and while common standard reference materials were not utilized at all institutions, internal standards and routine operational protocols were undertaken to assure analysis quality. More than 5000 analyses of plagioclase, pyroxene, olivine, and spinel are compiled here as a collaborative effort to provide mineral analyses to the expedition shipboard science party and other interested researchers.
ISSN: 1930-1014

hal-00464225
https://hal.archives-ouvertes.fr/hal-00464225
DOI : 10.2204/iodp.proc.304305.202.2009

Éditeur(s) : HAL CCSD Nature Publishing Group
Résumé : International audience
Temperature and fluid pressure conditions control rock deformation and mineralization on geological faults, and hence the distribution of earthquakes1. Typical intraplate continental crust has hydrostatic fluid pressure and a near-surface thermal gradient of 31 ± 15 degrees Celsius per kilometre2, 3. At temperatures above 300–450 degrees Celsius, usually found at depths greater than 10–15 kilometres, the intra-crystalline plasticity of quartz and feldspar relieves stress by aseismic creep and earthquakes are infrequent. Hydrothermal conditions control the stability of mineral phases and hence frictional–mechanical processes associated with earthquake rupture cycles, but there are few temperature and fluid pressure data from active plate-bounding faults. Here we report results from a borehole drilled into the upper part of the Alpine Fault, which is late in its cycle of stress accumulation and expected to rupture in a magnitude 8 earthquake in the coming decades4, 5. The borehole (depth 893 metres) revealed a pore fluid pressure gradient exceeding 9 ± 1 per cent above hydrostatic levels and an average geothermal gradient of 125 ± 55 degrees Celsius per kilometre within the hanging wall of the fault. These extreme hydrothermal conditions result from rapid fault movement, which transports rock and heat from depth, and topographically driven fluid movement that concentrates heat into valleys. Shear heating may occur within the fault but is not required to explain our observations. Our data and models show that highly anomalous fluid pressure and temperature gradients in the upper part of the seismogenic zone can be created by positive feedbacks between processes of fault slip, rock fracturing and alteration, and landscape development at plate-bounding faults.
ISSN: 0028-0836

hal-01685757
https://hal.archives-ouvertes.fr/hal-01685757
DOI : 10.1038/nature22355

Éditeur(s) : HAL CCSD Lippincott, Williams & Wilkins
Résumé : International audience
ISSN: 1044-3983

hal-00875980
https://hal.archives-ouvertes.fr/hal-00875980
DOI : 10.1097/EDE.0b013e318246073e
PUBMED : 22317818

Éditeur(s) : HAL CCSD Elsevier
Résumé : International audience
One of the main difficulties encountered when characterizing the hydrodynamic properties of a fractured aquifer is to identify the preferential flow paths within it. Different methods may be applied to determine the variability of the permeability at the borehole scale and to image the structure of the main flow zones between boreholes. In this paper, we compare the information obtained from different measurement techniques performed in a set of three 100 m depth wells (well-to-well spacing: 5­10 m) in a fractured crystalline rock setting. Geophysical logging and borehole-wall imaging are used to identify open and closed fractures intersecting the boreholes and their orientation. The comparison with flowmeter and single packer tests shows that few of the fractures interpreted as open from geophysical logs are significantly transmissive. Cross-borehole connectivity is first investigated from single packer tests with pressure monitoring in adjacent boreholes. To determine fracture zone connectivity, we propose a methodology simply based on the variation with packer depth of the ratio of the drawdown in the observation well and the drawdown in the pumping well. The results are compared to the analysis of cross-borehole flowmeter tests. We show that both methods provide consistent results with a similar level of information on connectivity.
ISSN: 0022-1694

insu-00221380
https://hal-insu.archives-ouvertes.fr/insu-00221380
DOI : 10.1016/j.jhydrol.2007.07.007

Éditeur(s) : HAL CCSD
Résumé : International audience
This paper introduces the information criteria in feature selection framework. Information criteria are integrated in feature selection scheme to select subset candidates. The accuracy of the proposed approach is based on the quality of the probability density approximations of these features. They are obtained using histograms optimized thanks to the adaptive arithmetic coding principles. Tests on simulated data and references are made. Multiple classifiers are used. The correct classification rate shows, the importance of this tools and its ability to select a best subsets. Generally, the subsets produce a good characterization of classes which the data belong.
TAIMA Proceedings

hal-00634754
https://hal.archives-ouvertes.fr/hal-00634754

Éditeur(s) : HAL CCSD Frontiers Media
Résumé : International audience
The yeast Candida glabrata has become the second cause of systemic candidemia in humans. However, relatively few genome-wide studies have been conducted in this organism and our knowledge of its transcriptional regulatory network is quite limited. In the present work, we combined genome-wide chromatin immunoprecipitation (ChIP-seq), transcriptome analyses, and DNA binding motif predictions to describe the regulatory interactions of the seven Yap (Yeast AP1) transcription factors of C. glabrata. We described a transcriptional network containing 255 regulatory interactions and 309 potential target genes. We predicted with high confidence the preferred DNA binding sites for 5 of the 7 CgYaps and showed a strong conservation of the Yap DNA binding properties between S. cerevisiae and C. glabrata. We provided reliable functional annotation for 3 of the 7 Yaps and identified for Yap1 and Yap5 a core regulon which is conserved in S. cerevisiae, C. glabrata, and C. albicans. We uncovered new roles for CgYap7 in the regulation of iron-sulfur cluster biogenesis, for CgYap1 in the regulation of heme biosynthesis and for CgYap5 in the repression of GRX4 in response to iron starvation. These transcription factors define an interconnected transcriptional network at the crossroads between redox homeostasis, oxygen consumption, and iron metabolism.
ISSN: 1664-302X

Droits : http://creativecommons.org/licenses/by/
hal-01323791
http://hal.upmc.fr/hal-01323791
http://hal.upmc.fr/hal-01323791/document
http://hal.upmc.fr/hal-01323791/file/fmicb-07-00645.pdf
DOI : 10.3389/fmicb.2016.00645

Éditeur(s) : HAL CCSD
Résumé : National audience
Le littoral, caractérisé par une grande diversitéd’environnements (plages sableuses ou rocheuses, cordonsdunaires, falaises, lagunes, estuaires...) est une zone très mobile etévolutive du fait de forçages hydrodynamiques et météorologiquessouvent intenses. Cet environnement est fragile, car soumis àl’impact d’activités humaines qui ne cessent d’augmenter (urbanisation,aménagements...), mais aussi aux effets du changementclimatique (élévation du niveau de la mer, modification durégime de houles, inondations). Comprendre le fonctionnementhydromorpho-sédimentaire des environnements littoraux est crucialpour prédire leur évolution et celle des écosystèmes associés.Le Service National d’Observation labellisé par le CNRS, (SNO)DYNALIT, - Dynamique du Littoral et du Trait de Côte a pourbut de constituer une base de données hydromorphologiques sur lelong terme de 29 sites ateliers représentatifs des environnementslittoraux français (côtes sableuses, falaises et embouchures) afin :- De mesurer la cinématique de ces environnements et du trait decôte associé ;- De mieux comprendre les interactions entre les processushydrodynamiques-transport sédimentaires-réponse morphologiqueassociés à la dynamique du littoral ;- Utiliser les observations pour améliorer notre connaissance desréponses de la morphologie des littoraux dans différents contextesde forçages météo-marins ;- D’utiliser ces observations pour analyser l’importance relativedes facteurs anthropiques et naturels sur la dynamique littorale ;- De se positionner en interlocuteur pour les responsables de ladéfinition et la mise en oeuvre des politiques de gestion des risqueslittoraux.
RST 2016, livre des résumés
Caen, France

insu-01387873
https://hal-insu.archives-ouvertes.fr/insu-01387873