Éditeur(s) : HAL CCSD Wiley
Résumé : 13 pages, 4 figures, to be published on J. Am. Cer. Soc
International audience
Since crack propagation in oxide materials at low crack velocities is partly determined by chemical corrosion, proper knowledge of the crack tip chemistry is crucial for understanding fracture in these materials. Such knowledge can be obtained only from in situ studies because the processes that occur in the highly confined environment of the crack tip are very different from those that take place at free surfaces, or that can be traced post mortem. We report the occurrence of hydrous liquid condensate between the two fracture surfaces in the vicinity of the tip of tensile cracks in silica. Observations are performed in real-time by means of atomic force microscopy (AFM) at continuously controlled crack velocities in the regime of stress corrosion. Condensate formation and changes in extent and shape are demonstrated for a wide range of macroscopic humidity at different crack speeds. Its liquid character is confirmed by the study of AFM phase-contrast data. It is believed that this evidence of a nanoscale liquid hydrous phase at the crack tip will enable novel insights in the chemistry of failure of oxide materials.
ISSN: 0002-7820

hal-00424984
https://hal.archives-ouvertes.fr/hal-00424984
ARXIV : cond-mat/0509209
DOI : 10.1111/j.1551-2916.2005.00765.x

Éditeur(s) : HAL CCSD American Geophysical Union
Résumé : International audience
The dynamics of mantle plumes and the origin of their associated swells remain some of the most controversial topics in geodynamics. Here we construct a numerical model of the mantle flow beneath the French Polynesia region. Our study is based on a new regional seismic tomography model, which high resolution allows obtaining information at the scale of plumes. We find excellent correlations between the observed and the modeled dynamic swells, between the modeled flow pattern and the active volcanism and between the buoyancy fluxes obtained from our numerical model and the ones deduced from the swells morphology. These outstanding fits reveal for the first time that a direct link exists between the surface observations and mantle flows.
ISSN: 0094-8276

hal-01236157
http://hal.univ-reunion.fr/hal-01236157
http://hal.univ-reunion.fr/hal-01236157/document
http://hal.univ-reunion.fr/hal-01236157/file/Adam_South_Pacific_Superswell_GRL2010.pdf
DOI : 10.1029/2010GL042534

Éditeur(s) : HAL CCSD American Society for Microbiology
Résumé : International audience
: We investigated the in vitro effects of four alkyl-galactofuranoside derivatives, i.e. octyl-β-D-galactofuranoside (1), 6-amino-β-D-galactofuranoside (2), 6-N-acetamido-β-D-galactofuranoside (3), and 6-azido-β-D-galactofuranoside (4) on Leishmania donovani. Their mechanism of action was explored using electron paramagnetic resonance spectroscopy (EPR) and nuclear magnetic resonance (NMR), and ultrastructural alterations by transmission electron microscopy (TEM). Compound 1 showed the most promising effects by inhibiting promastigote growth at IC50 8.96 ± 2.5 μM. All compounds exhibit low toxicity towards human macrophages. Compound 1 had a higher selectivity index than the comparative molecule used, i.e. miltefosine (159.7 versus 37.9, respectively). EPR showed that compound 1 significantly reduced membrane fluidity, compared to control promastigotes and to compound 3. The furanose ring was shown to support this effect since the isomer galactopyranose had no effect on parasite membrane fluidity or growth. NMR showed a direct interaction of all compounds (1>2>3>4) with the promastigote membrane, as well as with octyl-galactopyranose and octanol, providing evidence that the n-octyl chain was primarily involved in the anchoring with the parasite membrane, followed by the putative crucial role of furanose ring in the anti-leishmanial activity. A morphologic analysis of compound 1-treated promastigotes by TEM revealed profound alterations on parasite membrane and organelles, but not with compound 3. Quantification of annexinV binding by flow cytometry confirmed that compound 1 induced apoptosis in >90% promastigotes. The effect of compound 1 was also assessed on intra-macrophagic amastigotes, and showed a reduction in amastigote growth associated with an increase of ROS production, thus validating its promising effect.
ISSN: 0066-4804

hal-00941772
https://hal.archives-ouvertes.fr/hal-00941772
PUBMED : 24468785
DOI : 10.1128/AAC.01350-13

Éditeur(s) : HAL CCSD Elsevier
Résumé : International audience
Herbicides are generally the most extensively used of the pesticides applied to agricultural crops. However, the literature contains little evidence useful in assessing the potential sources of the general population's exposure to herbicides, including by residential proximity to crops. The objective of this study was to take advantage of data from the PELAGIE mother-child cohort to identify the main determinants of the body burden of exposure to the chloroacetanilide and triazine herbicides commonly used on corn crops in Brittany, France, before 2006. Urine samples from a randomly selected subcohort of women in the first trimester of pregnancy (n=579) were assayed for herbicide metabolites. The residential exposure resulting from proximity to corn crops was assessed with satellite-image-based scores combined with meteorological data. Data on diet, drinking tap water (from the public water supply), occupations, and household herbicide use were collected by questionnaires. Herbicides were quantified in 5.3% to 39.7% of urine samples. Alachlor and acetochlor were found most frequently in the urine of women living in rural areas. The presence of dealkylated triazine metabolites in urine samples was positively associated with residential proximity to corn crops (OR=1.38, 95% CI: 1.05-1.80). Urinary metabolites of both atrazine and dealkylated triazine were correlated with tap water consumption (OR=2.94, 1.09-7.90, and OR=1.82, 1.10-3.03, respectively); hydroxylated triazine metabolites were correlated with fish intake (OR=1.48, 1.09-1.99). This study reinforces previous results that suggest that environmental contamination resulting from agricultural activities may contribute to the general population's exposure to herbicides.
Environment International

hal-00966842
https://hal.archives-ouvertes.fr/hal-00966842
https://hal.archives-ouvertes.fr/hal-00966842/document
DOI : 10.1016/j.envint.2013.10.010
PUBMED : 24246238

Éditeur(s) : HAL CCSD
Résumé : International audience
Background: Everyone is subject to environmental exposures from various sources, with negative health impacts (air, water and soil contamination, noise...) or with positive effects (e.g. green space). Studies considering such complex environmental settings in a global manner are rare.Aims: We propose to use data mining techniques to create a composite exposure index with a data-driven approach, in view to assess the environmental burden experienced by populations. We illustrate this approach on a large French metropolitan area.Methods: The study was carried out in the Great Lyon area (France, 1,260,348 inhabitants in 2007, 527 km²) at the census block group (BG) scale. Indicators on NO2 annual concentrations, noise levels, proximity to green spaces and to industrial plants, and road traffic were synthetized using Multiple Factor Analysis (MFA), which allows to explore the relations between exposures and BGs without a priori knowledge, and to synthetize indicators of different types. Hierarchical clustering was then used to create BG classes. Results: The four first components of the MFA explained respectively 23, 14, 13 and 12% of the total variance. Clustering in 3 classes group: 1) BGs far from industries, greener and with less noise and air pollution than the average; 2) BGs close to industries with less green spaces and air pollution than the average; and 3)BGs far from industries but with higher levels of noise, air pollution and traffic. Greater numbers of classes were tested in order to assess a variety of clustering.Conclusions: We present a data driven approach using data mining techniques, which seem overlooked for cumulative exposure assessment in complex environmental settings. Although it cannot be applied directly for risk or health effect assessment, the resulting index can help to identify hot spots of cumulative exposure, to prioritize urban policies or to compare the environmental burden across study areas in an epidemiological framework.
Environment and Health – Bridging South, North, East and West. Conference of ISEE, ISES and ISIAQ.
Basel, Switzerland

hal-01432884
https://hal.archives-ouvertes.fr/hal-01432884
https://hal.archives-ouvertes.fr/hal-01432884/document
https://hal.archives-ouvertes.fr/hal-01432884/file/Poster_2013_ISEE_20130711.pdf

Éditeur(s) : HAL CCSD Centers for Disease Control and Prevention
Résumé : International audience
To assess the prevalence of malaria among illegal gold miners in the French Guiana rainforest, we screened 205 miners during May-June 2014. Malaria prevalence was 48.3%; 48.5% of cases were asymptomatic. Patients reported self-medication with artemisinin-based combination therapy. Risk for emergence and spread of artemisinin resistance among gold miners in the rainforest is high.
ISSN: 1080-6040

Droits : http://creativecommons.org/licenses/by/
pasteur-01305106
https://hal-pasteur.archives-ouvertes.fr/pasteur-01305106
https://hal-pasteur.archives-ouvertes.fr/pasteur-01305106/document
https://hal-pasteur.archives-ouvertes.fr/pasteur-01305106/file/15-1957.pdf
DOI : 10.3201/eid2205.151957.

Éditeur(s) : HAL CCSD Elsevier
Résumé : A Ar-40/Ar-39 geochronological study was performed on amphibole and biotite from some representative units of distinct tectonic domains of the southeastern Guiana Shield, north of the Amazonian Craton, the Amapa Block and the Carecuru Domain. In the AmapA Block, an Archean continental block involved in the Transamazonian orogenesis (2.26-1.95 Ga), the investigated minerals, from rocks of the Archean high-grade basement assemblage, give only Paleoproterozoic ages, indicating their complete resetting during the Transamazonian orogenic event. Amphibole ages vary from 2087 +/- 3 to 2047 +/- 20 Ma, and biotite ages spread mainly between 2079 +/- 18 and 2033 +/- 13 Ma. In the Carecuru Domain, in which the geodynamic evolution is related to Paleoproterozoic magmatic arc setting during the Transamazonian event, calc-alkaline granitoids yield amphibole age of 2074 +/- 17 Ma, and biotite ages of 1928 +/- 19 Ma and 1833 +/- 13 Ma. These data reinforce the importance of the Transamazonian orogenic cycle in the investigated area, and indicate that the rocks were not significantly affected by post-Transamazonian events. When coupled with available U-Th-Pb monazite and Pb-Pb zircon geochronological records and petro-structural observations, the new Ar-40/Ar-39 data delineate contrasting cooling and exhumation histories for the tectonic domains. In the AmapA Block, the data suggest nearly vertical T-t paths that reflect fast cooling rates, which indicate tectonically controlled exhumation, related to collisional stages of the Transamazonian event, between 2.10 and 2.08 Ga. Conversely, in the Carecuru Domain, low cooling rates suggest that the arc-related granitoids underwent slow and monotonous cooling since their emplacement until reaching the biotite isotopic closure temperature.
ISSN: 0895-9811

hal-00420075
https://hal.archives-ouvertes.fr/hal-00420075
DOI : 10.1016/j.jsames.2008.08.010

Éditeur(s) : HAL CCSD
Résumé : International audience
o abstract
Coastal Engineering Proceedings

hal-01426586
https://hal.archives-ouvertes.fr/hal-01426586

Éditeur(s) : HAL CCSD Elsevier
Résumé : International audience
This study investigates if acclimatization to residual pesticide contamination in agricultural soils is reflected in detoxification, antioxidant enzyme activities and energy budget of earthworms. Five fields within a joint agricultural area exhibited different chemical and farming histories from conventional cultivation to organic pasture. Soil multiresidual pesticide analysis revealed up to 9 molecules including atrazine up to 2.4 ng g(-1) dry soil. Exposure history of endogeic Aporrectodea caliginosa and Allolobophora chlorotica modified their responses to pesticides. In the field, activities of soluble glutathione-S-transferases (sGST) and catalase increased with soil pesticide contamination in A. caliginosa. Pesticide stress was reflected in depletion of energy reserves in A. chlorotica. Acute exposure of pre-adapted and naïve A. caliginosa to pesticides (fungicide Opus(®), 0.1 μg active ingredient epoxiconazole g(-1) dry soil, RoundUp Flash(®), 2.5 μg active ingredient glyphosate g(-1) dry soil, and their mixture), revealed that environmental pre-exposure accelerated activation of the detoxification enzyme sGST towards epoxiconazole.
ISSN: 0269-7491

hal-01063923
https://hal.archives-ouvertes.fr/hal-01063923
DOI : 10.1016/j.envpol.2014.05.001
PUBMED : 24874794

Éditeur(s) : HAL CCSD BioMed Central
Résumé : International audience
Background: End-Stage renal disease (ESRD) causes considerable morbidity and mortality, and significantly alterspatients’ quality of life. There are very few published data on this problem in the French Overseas territories. Thedevelopment of a registry on end stage renal disease in French Guiana in 2011 allowed to describe the magnitudeof this problem in the region for the first time.Methods: Using data from the French Renal Epidemiology and Information Network registry (R.E.I.N). Descriptivestatistics on quantitative and qualitative variables in the registry were performed on prevalent cases and incidentcases in 2011, 2012 and 2013.Results: French Guiana has one of the highest ESRD prevalence and incidence in France. The two main causes ofESRD were hypertensive and diabetic nephropathies. The French Guianese population had a different demographicprofile (younger, more women, more migrants) than in mainland France. Most patients had at least one comorbidity,predominantly (95.3%) hypertension. In French Guiana dialysis was initiated in emergency for 71.3% of patients versus33% in France (p < 0.001).Conclusion: These first results give important public health information: i) End stage renal disease has a very highprevalence relative to mainland France ii) Patients have a different demographic profile and enter care late in thecourse of their renal disease. These data are closer to what is observed in the Caribbean or in Latin America than inMainland France.
ISSN: 1471-2369

inserm-01567222
http://www.hal.inserm.fr/inserm-01567222
http://www.hal.inserm.fr/inserm-01567222/document
http://www.hal.inserm.fr/inserm-01567222/file/2017%2C%20Rochemont%20-%20End%20stage%20renal%20disease.pdf
DOI : 10.1186/s12882-017-0614-6

Éditeur(s) : HAL CCSD American Society of Hematology
Résumé : International audience
ISSN: 0006-4971

inserm-00919490
http://www.hal.inserm.fr/inserm-00919490
http://www.hal.inserm.fr/inserm-00919490/document
http://www.hal.inserm.fr/inserm-00919490/file/2013_Lemonne_-_Does_increased_red_blood_cell_deformability_raise_the_risk_for_osteonecrosis_in_sickle_cell_anemia.pdf
http://www.hal.inserm.fr/inserm-00919490/file/inserm-00919490_edited.pdf

Éditeur(s) : HAL CCSD Springer Verlag
Résumé : International audience
Investigation of an eclogite xenolith, discovered in a Cretaceous granite from the Central Domain of the Dabieshan massif in eastern China, yields new petrological insights into the high to ultrahigh-pressure metamorphism, experienced by the Qinling-Dabie orogen. Prior to inclusion as a xenolith in the granite during the Early Cretaceous, this eclogite xenolith had recorded a complex metamorphic evolution that complies with subduction and exhumation processes experienced by the continental crust of the South China Block. Well-preserved mineral parageneses substantiate the prograde and retrograde stages revealed by inclusions in porphyroblastic garnet and zoned minerals such as garnet, comphacite and amphibole in the matrix. The relatively low P/T re-equilibration during a latemetamorphic stage was textually inferred by the presence of aluminous and calcic-subcalcic amphiboles such as katophorite, edenite, taramite and pargasite as main matrix phases. According to our U/Pb, Rb/Sr and new 40Ar/39Ar geochronological results, namely 109 ± 1 and 112 ± 2 Ma plateau ages for muscovite and amphiboles, respectively, two successive but distinct cooling stages account for the thermal history of the granite–migmatite gneiss dome that forms the Central Dabieshan Domain. We argue that prior to the Cretaceous doming, the Central Dabieshan Domain experienced a tectono-metamorphic evolution similar to that observed in the high-pressure to ultra high-pressure units developed in the Southern Dabieshan Domain and Hong'an massif.
ISSN: 1437-3254

hal-00104224
https://hal-insu.archives-ouvertes.fr/hal-00104224
https://hal-insu.archives-ouvertes.fr/hal-00104224/document
https://hal-insu.archives-ouvertes.fr/hal-00104224/file/Lin-IntJEarthSciences-2007.pdf
DOI : 10.1007/s00531-006-0129-z

Éditeur(s) : HAL CCSD De Gruyter
Résumé : International audience
The data available in the literature concerning wood cutting forces permit to build models or to simulate the main wood machining processes (milling, sawing, peeling etc.). This approach contributes to a better understanding of formation of wood surfaces and chips and the data may be helpful to optimize cutting geometry, reduce tool wear, improve tool material, and to size tool-machines. The models may also be useful for industrial application in two ways: (1) providing data to optimise the settings for a given operation (batch approach) and (2) building predictive models that could be the basis of an online control systems for the machining processes (interactive approach). A prerequisite for this is that numerous machining tests on different wood materials are performed based on experiences with different kind of tools and experimental devices. With potential industrial applications in focus, the emphasis of this review was on the wood peeling process, which is a very demanding special case of wood cutting. Though not so many industrial machines are equipped with expensive force sensors, there is a lot of high quality information available about cutting forces which may be useful to improve the scientific or technologic knowledge in wood machining. Alternative parameters, such as vibration or sound measurements, appear to be promising substitutes in the praxis, particularly to feed online control systems of any wood cutting process.
ISSN: 0018-3830

hal-00876003
https://hal.archives-ouvertes.fr/hal-00876003
https://hal.archives-ouvertes.fr/hal-00876003/document
https://hal.archives-ouvertes.fr/hal-00876003/file/LABOMAP_Holzforschung_2009_denaud.pdf
ENSAM : http://hdl.handle.net/10985/7419

Éditeur(s) : HAL CCSD Wiley Open Access
Résumé : International audience
Estetrol (E4) is a natural estrogen with a long half-life produced only by the human fetal liver during pregnancy. The crystal structures of the estrogen receptor α (ERα) ligand-binding domain bound to 17β-estradiol (E2) and E4 are very similar, as well as their capacity to activate the two activation functions AF-1 and AF-2 and to recruit the coactivator SRC3. In vivo administration of high doses of E4 stimulated uterine gene expression, epithelial proliferation, and prevented atheroma, three recognized nuclear ERα actions. However, E4 failed to promote endothelial NO synthase activation and acceleration of endothelial healing, two processes clearly dependent on membrane-initiated steroid signaling (MISS). Furthermore, E4 antagonized E2 MISS-dependent effects in endothelium but also in MCF-7 breast cancer cell line. This profile of ERα activation by E4, uncoupling nuclear and membrane activation, characterizes E4 as a selective ER modulator which could have medical applications that should now be considered further.
ISSN: 1757-4676

hal-01118028
https://hal-univ-rennes1.archives-ouvertes.fr/hal-01118028
PUBMED : 25214462
PUBMEDCENTRAL : PMC4287935
DOI : 10.15252/emmm.201404112

Éditeur(s) : HAL CCSD International Medical Press
Résumé : International audience
Background: Although the role of clinical/biological factors associated with mortality has already been explored in HIV-infected patients on antiretroviral therapy (ART), to date little attention has been given to the potential role of social vulnerability. This study aimed to construct an appropriate measure of social vulnerability and to evaluate whether this measure is predictive of increased mortality risk in ART-treated patients followed up in the ANRS CO8 APROCO-COPILOTE cohort.Methods: The cohort enrolled 1,281 patients initiating a protease inhibitor-based regimen in 1997–1999. Clinical/laboratory data were collected every 4 months. Self-administered questionnaires collected psycho-social/behavioural characteristics at enrolment (month [M] 0), M4 and every 8–12 months thereafter. A multiple correspondence analysis using education, employment and housing indicators helped construct a composite indicator measuring social vulnerability. The outcome studied was all-cause deaths occurring after M4. The relationship between social vulnerability and mortality, after adjustment for other predictors, was studied using a shared-frailty Cox model, taking into account informative study dropout.Results: Over a median (IQR) follow-up of 7.9 (3.0–11.2) years, 121 deaths occurred among 1,057 eligible patients, corresponding to a mortality rate (95% CI) of 1.64 (1.37, 1.96)/100 person-years. Leading causes of death were non-AIDS defining cancers (n=26), AIDS (n=23) and cardiovascular diseases (n=12). Social vulnerability (HR [95% CI] =1.2 [1.0, 1.5]) was associated with increased mortality risk, after adjustment for other known behavioural and bio-medical predictors.Conclusions: Social vulnerability remains a major mortality predictor in ART-treated patients. A real need exists for innovative interventions targeting individuals cumulating several sources of social vulnerability, to ensure that social inequalities do not continue to lead to higher mortality.
ISSN: 1359-6535

hal-01299300
https://hal-univ-rennes1.archives-ouvertes.fr/hal-01299300
https://hal-univ-rennes1.archives-ouvertes.fr/hal-01299300/document
https://hal-univ-rennes1.archives-ouvertes.fr/hal-01299300/file/Twelve-year%20mortality%20in%20HIV-infected%20patients_accepted.pdf
DOI : 10.3851/IMP2960

Éditeur(s) : HAL CCSD Public Library of Science
Résumé : International audience
A thorough phytochemical study of Stereocaulon evolutum was conducted, for the isolation of structurally related atranorin derivatives. Indeed, pilot experiments suggested that atra-norin (1), the main metabolite of this lichen, would interfere with the lifecycle of hepatitis C virus (HCV). Eight compounds, including one reported for the first time (2), were isolated and characterized. Two analogs (5, 6) were also synthesized, to enlarge the panel of atra-norin-related structures. Most of these compounds were active against HCV, with a half-maximal inhibitory concentration of about 10 to 70 µM, with depsides more potent than monoaromatic phenols. The most effective inhibitors (1, 5 and 6) were then added at different steps of the HCV lifecycle. Interestingly, atranorin (1), bearing an aldehyde function at C-3, inhibited only viral entry, whereas the synthetic compounds 5 and 6, bearing a hydroxy-methyl and a methyl function, respectively, at C-3 interfered with viral replication.
ISSN: 1932-6203

hal-01134343
https://hal-univ-rennes1.archives-ouvertes.fr/hal-01134343
https://hal-univ-rennes1.archives-ouvertes.fr/hal-01134343/document
https://hal-univ-rennes1.archives-ouvertes.fr/hal-01134343/file/Depsides-PloS.pdf
DOI : 10.1371/journal.pone.0120405

Éditeur(s) : HAL CCSD Elsevier
Résumé : International audience
Because earthworms sustain soil functioning and fertility, there is a need to advance the knowledge of their adaptation potential to chemicals in anthropogenic landscapes. Our hypothesis is that there is acclimation to organic chemicals (pesticides) in earthworms that durably persist under conventional farming in anthropogenic landscapes. The adaptation capability of two populations of earthworms (Aporectodea caliginosa) having a different chemical exposure history, - one originating from 20 years of organic farming (naïve population) and another from 20 years of conventional farming (pre-exposed population) - to cope with soil organic pollutant (Opus®, epoxiconazole a worldwide used fungicide) were investigated. Several complementary metabolic and energetic endpoints were followed, and cast production was assessed as a behavioural biomarker related to earthworms ecological role for the soil. Basal metabolism reflected by respiration rate was increased in both fungicide-exposed worms compared to controls. Glycogen resources were decreased in the same proportion in the two populations but more rapidly for the naïve (7 days) than for the pre-exposed population (28 days). Soluble protein and most amino-acids contents increased in the pre-exposed population only, suggesting a detoxification mechanism. Metabolomic profiles showed a cut-off between fungicide-exposed and control groups in the pre-exposed earthworms only, with an increase in most of the metabolites. Exposure to a low dose of epoxiconazole increased cast production of pre-exposed earthworms, and this resulted in an increase in pesticide disappearance. As far as we know, this is the first study which evidenced there is an acclimation to an agricultural chemical in earthworms derived from conventional farming that also relates to a change in their burrowing behaviour, and for which larger consequences for the soil ecosystem need to be addressed. This original finding is of major interest in the frame of ecosystem resilience to global changes. Whether this physiological adaptation is a general pattern of response against fungicides or other pesticides would need to be confirmed with other molecules and agricultural contexts.
Soil Biology and Biochemistry

hal-01005821
https://hal-univ-rennes1.archives-ouvertes.fr/hal-01005821
https://hal-univ-rennes1.archives-ouvertes.fr/hal-01005821/document
DOI : 10.1016/j.soilbio.2014.01.032

Éditeur(s) : HAL CCSD Wiley/Blackwell
Résumé : International audience
This article documents the addition of 512 microsatellite marker loci and nine pairs of Single Nucleotide Polymorphism (SNP) sequencing primers to the Molecular Ecology Resources Database. Loci were developed for the following species: Alcippe morrisonia morrisonia, Bashania fangiana, Bashania fargesii, Chaetodon vagabundus, Colletes floralis, Coluber constrictor flaviventris, Coptotermes gestroi, Crotophaga major, Cyprinella lutrensis, Danaus plexippus, Fagus grandifolia, Falco tinnunculus, Fletcherimyia fletcheri, Hydrilla verticillata, Laterallus jamaicensis coturniculus, Leavenworthia alabamica, Marmosops incanus, Miichthys miiuy, Nasua nasua, Noturus exilis, Odontesthes bonariensis, Quadrula fragosa, Pinctada maxima, Pseudaletia separata, Pseudoperonospora cubensis, Podocarpus elatus, Portunus trituberculatus, Rhagoletis cerasi, Rhinella schneideri, Sarracenia alata, Skeletonema marinoi, Sminthurus viridis, Syngnathus abaster, Uroteuthis (Photololigo) chinensis, Verticillium dahliae, Wasmannia auropunctata, and Zygochlamys patagonica. These loci were cross-tested on the following species: Chaetodon baronessa, Falco columbarius, Falco eleonorae, Falco naumanni, Falco peregrinus, Falco subbuteo, Didelphis aurita, Gracilinanus microtarsus, Marmosops paulensis, Monodelphis Americana, Odontesthes hatcheri, Podocarpus grayi, Podocarpus lawrencei, Podocarpus smithii, Portunus pelagicus, Syngnathus acus, Syngnathus typhle,Uroteuthis (Photololigo) edulis, Uroteuthis (Photololigo) duvauceli and Verticillium albo-atrum. This article also documents the addition of nine sequencing primer pairs and sixteen allele specific primers or probes for Oncorhynchus mykiss and Oncorhynchus tshawytscha; these primers and assays were cross-tested in both species.
ISSN: 1755-098X

hal-01094222
https://hal-agroparistech.archives-ouvertes.fr/hal-01094222
DOI : 10.1111/j.1755-0998.2010.02916.x

Éditeur(s) : HAL CCSD BioMed Central
Résumé : International audience
BACKGROUND: Environmental exposures, including dietary contaminants, may influence the developing immune system. This study assesses the association between maternal pre-parturition consumption of seafood and wheeze, eczema, and food allergy in preschool children. Fish and shellfish were studied separately as they differ according to their levels of omega-3 polyunsaturated fatty acids (which have anti-allergic properties) and their levels of contaminants. METHODS: The PELAGIE cohort included 3421 women recruited at the beginning of pregnancy. Maternal fish and shellfish intake was measured at inclusion by a food frequency questionnaire. Wheeze, eczema, and food allergy were evaluated by a questionnaire completed by the mother when the child was 2 years old (n = 1500). Examination of the associations between seafood intake and outcomes took major confounders into account. Complementary sensitivity analyses with multiple imputation enabled us to handle missing data, due mostly to attrition. RESULTS: Moderate maternal pre-parturition fish intake (1 to 4 times a month) was, at borderline significance, associated with a lower risk of wheeze (adjusted OR = 0.69 (0.45-1.05)) before age 2, compared with low intake (< once/month). This result was not, however, consistent: after multiple imputation, the adjusted OR was 0.86 (0.63-1.17). Shellfish intake at least once a month was associated with a higher risk of food allergy before age 2 (adjusted OR = 1.62 (1.11-2.37)) compared to low or no intake (< once/month). Multiple imputation confirmed this association (adjusted OR = 1.52 (1.05-2.21)). CONCLUSIONS: This study suggests that maternal pre-parturition shellfish consumption may increase the risk of food allergy. Further large-scale epidemiologic studies are needed to corroborate these results, identify the contaminants or components of shellfish responsible for the effects observed, determine the persistence of the associations seen at age 2, and investigate potential associations with health effects observable at later ages, such as allergic asthma.
ISSN: 1476-069X

Droits : info:eu-repo/semantics/OpenAccess
inserm-00926282
http://www.hal.inserm.fr/inserm-00926282
http://www.hal.inserm.fr/inserm-00926282/document
http://www.hal.inserm.fr/inserm-00926282/file/1476-069X-12-102.pdf
DOI : 10.1186/1476-069X-12-102

Éditeur(s) : HAL CCSD Elsevier - International Brain Research Organization
Résumé : International audience
Women are more likely to develop depression during childbearing years with up to 20% of women suffering from depression during pregnancy and in the postpartum period. Increased prevalence of depression during the perinatal period has resulted in frequent selective serotonin reuptake inhibitor (SSRI) antidepressant treatment; however the effects of such medications on the maternal brain remain limited. Therefore, the aim of the present study is to investigate the effects of the SSRI medication, fluoxetine, on neurobiological differences in the maternal brain. To model aspects of maternal depression, gestational stress was used. Sprague-Dawley rat dams were exposed to either gestational stress and/or fluoxetine (5mg/kg/day) to form the following four groups: 1. Control+Vehicle, 2. Stress+Vehicle, 3. Control+Fluoxetine 4. Stress+Fluoxetine. At weaning maternal brains were collected. Main findings show that gestational stress alone increased synaptophysin and serotonin metabolism in the CG2 region of the cortex while fluoxetine treatment after stress normalized these effects. In the hippocampus, fluoxetine treatment, regardless of gestational stress exposure, decreased both global measures of methylation in the dentate gyrus, as measured by Dnmt3a immunoreactivity, as well as serotonin metabolism. No further changes in synaptophysin, PSD-95, or Dnmt3a immunoreactivity were seen in the cortical or hippocampal areas investigated. These findings show that gestational stress and SSRI medication affect the neurobiology of the maternal brain in a region-specific manner. This work adds to a much needed area of research aimed at understanding neurobiological changes associated with maternal depression and the role of SSRI treatment in altering these changes in the female brain
ISSN: 0306-4522

hal-01301509
https://hal-univ-rennes1.archives-ouvertes.fr/hal-01301509
https://hal-univ-rennes1.archives-ouvertes.fr/hal-01301509/document
https://hal-univ-rennes1.archives-ouvertes.fr/hal-01301509/file/Gestational%20stress%20and%20fluoxetine%20treatment%20%281%29.pdf
PUBMED : 27060483