Éditeur(s) : HAL CCSD Oxford University Press (OUP)
Résumé : International audience
The organogenesis, histogenesis and growth of larvae of the fighting conch Strombus pugilis (Linne, 1758) were studied over a period of 30 d after hatching in laboratory culture. Early development of S. pugilis was examined by light and scanning electron microscopy. Rearing was conducted at 27 +/- 1 degrees C. Veligers were reared at 200 larvae l(-1) in 4-l containers. Larvae were fed with the microalgae Isochrysis galbana and Nanochloropsis oculata at a concentration of 1,000 cell l(-1). The protoconch at hatching measured 212 +/- 12.14 mu m in length and the shell reached 1,100 +/- 29.11 mu m 29 d after hatching. Development characteristics are described from hatching to settlement. Newly hatched veligers possess two velar lobes, a larval shell consisting of 1.5 whorls, eyespots and a single right tentacle. Late veligers (5-d old larvae) have four velar lobes and two shell whorls and the left tentacle appears. Pediveligers show a functional adult heart at 11 d. Crawling behaviour and settlement were observed from 27 to 31 d. Plantigrades were observed after 29 d, when a functioning proboscis is observed and the velar lobes are lost. This study will facilitate the identification of gastropod larval shells in the plankton and of juveniles in the meiobenthos and will aid aquaculture of Strombus species.
ISSN: 0260-1230

hal-01545910
https://hal.archives-ouvertes.fr/hal-01545910
DOI : 10.1093/mollus/eyv011

Éditeur(s) : HAL CCSD
Résumé : Point processes are often used as spatial or spatio-temporal distribution models of occurrences. In this PhD dissertation, we focus first on Cox processes driven by a hidden process associated with a Dirichlet process. This model corresponds to hidden occurrences influencing the stochastic intensity of observed occurrences. We generalize the notion of "Shot noise Cox process" introduced by M{o}ller and develop its bayesian analysis by a Gibbs sampler combined with a Metropolis-Hastings algorithm. We show that our MCMC method is a reversible jump one. The model takes into account a random number of hidden contributions producing effects on the observed point process intensity. Therefore the parameter space has a variable dimension. We focus the statistical inference on the estimation of the hidden contribution expected value, the hidden contribution expected number, the spatial influence and correlation parameters. The contribution equality test and contribution independence test are proposed. Applications in epidemiology and ecology are shown from extit{Rubus fruticosa} data, extit{Ibicella lutea} data and death number data in counties of Georgia, USA. Two situations are considered with respect to available data~: firstly, the spatial positions of occurrences are observed between several pairs of consecutive dates; secondly, counts are carried out over a fixed time interval in several spatial sampling units. Secondly, we focus on point processes with memory introduced by Kagan, Ogata and Vere-Jones, pioneers in statistical seismology. In fact, spatio-temporal point processes play an important role in the studies of earthquake catalogs since they consist of seismic events with their dates and spatial locations. We studied an ETAS (Epidemic Type Aftershock Sequence) model with time independent background intensity and several triggering functions taking into account previous events. We illustrate our approach with a seismicity study of the Lesser Antilles arc. A comparaison study of Gamma, Weibull, Log-Normal and modified Omori law triggering function models is also carried out. We show that the modified Omori law does not fit the Lesser Antilles seismic data and the best adjusted triggering function is the Weibull model. Consequently, the waiting time between aftershocks is weaker in the Lesser Antilles zone compared to the one in regions with seismicity described by the modified Omori law. In other words, aftershock aggregativity is higher in the Lesser Antilles region. The stochastic background intensity following a Dirichlet process centered on a spatial log-normal process is discussed.
Les processus ponctuels sont souvent utilisés comme modèles de répartitions spatiales ou spatio-temporelles d'occurrences. Dans cette thèse, nous nous intéressons tout d'abord à des processus de Cox dirigés par un processus caché associé à un processus de Dirichlet. Ce modèle correspond à des occurrences cachées influençant l'intensité stochastique des occurrences observées. Nous généralisons la notion de " Shot noise Cox process " introduite par Moller et développons le traitement bayésien par un échantillonneur de Gibbs combiné à un algorithme de Metropolis-Hastings. Nous montrons que cette méthode MCMC est à sauts réversibles. Le modèle prend en compte, en effet, un nombre aléatoire de contributions cachées influençant l'intensité du processus ponctuel observé donc a un espace paramétrique de dimension variable. Nous focalisons l'inférence statistique sur l'estimation de la valeur espérée de chaque contribution cachée, le nombre espéré de contributions cachées, le degré d'influence spatiale de ces contributions et leur degré de corrélation. Le test d'égalité des contributions et celui de leur indépendance sont ainsi développés. L'utilité en épidémiologie et en écologie est alors démontrée à partir de données de Rubus fruticosa, Ibicella lutea et de mortalité dans les cantons de Georgia, USA. En termes de données observées, deux situations sont considérées: premièrement, les positions spatiales des occurrences sont observées entre plusieurs paires de dates consécutives; deuxièmement, des comptages sont effectués, au cours d'une période fixée, dans des unités d'échantillonnage spatiales. D'autre part, nous nous intéressons aux processus ponctuels à mémoire introduits par Kagan, Ogata et Vere-Jones, précurseurs de la statistique sismologique. En effet, les processus ponctuels spatio-temporels ont une place importante dans l'étude des catalogues sismiques puisque ces derniers sont généralement constitués d'événements sismiques datés et géo-référencés. Nous avons étudié un modèle ETAS (Epidemic Type Aftershock Sequence) avec une intensité d'arrière-plan indépendante du temps et plusieurs fonctions déclenchantes permettant d'intégrer les événements antérieurs récents. Cette approche est utilisée pour étudier la sismicité de l'arc des Petites Antilles. Une étude comparative des modèles Gamma, Weibull, Log-Normal et loi d'Omori modifiée pour les fonctions déclenchantes est menée. Nous montrons que la loi d'Omori modifiée ne s'ajuste pas aux données sismiques des Petites Antilles et la fonction déclenchante la plus adaptée est le modèle de Weibull. Cela implique que le temps d'attente entre répliques dans la zone des Petites Antilles est plus faible que celui des régions à sismicité décrite par la loi d'Omori modifiée. Autrement dit, l'agrégation des répliques après un événement majeur est plus prononcée dans la zone des Petites Antilles. La possibilité d'inclure une intensité d'arrière-plan suivant un processus de Dirichlet centré sur un processus spatial log-gaussien est discutée.
https://tel.archives-ouvertes.fr/tel-00841146

Droits : info:eu-repo/semantics/OpenAccess
NNT : 2012AGUY0555
tel-00841146
https://tel.archives-ouvertes.fr/tel-00841146
https://tel.archives-ouvertes.fr/tel-00841146/document
https://tel.archives-ouvertes.fr/tel-00841146/file/these_VALMY.pdf

Éditeur(s) : HAL CCSD American Chemical Society
Résumé : International audience
Childhood absence epilepsy is a prototypic form of generalized nonconvulsive epilepsy characterized by short impairments of consciousness concomitant with synchronous and bilateral spike-and-wave discharges in the electroencephalogram. For scientists in this field, the BS/Orl and BR/Orl mouse lines, derived from a genetic selection, constitute an original mouse model "in mirror" of absence epilepsy. The potential of MALDI imaging mass spectrometry (IMS) for the discovery of potential biomarkers is increasingly recognized. Interestingly, statistical analysis tools specifically adapted to IMS data sets and methods for the identification of detected proteins play an essential role. In this study, a new cross-classification comparative design using a combined discrete wavelet transformation-support vector machine classification was developed to discriminate spectra of brain sections of BS/Orl and BR/Orl mice. Nineteen m/z ratios were thus highlighted as potential markers with very high recognition rates (87-99%). Seven of these potential markers were identified using a top-down approach, in particular a fragment of Synapsin-I. This protein is yet suspected to be involved in epilepsy. Immunohistochemistry and Western Blot experiments confirmed the differential expression of Synapsin-I observed by IMS, thus tending to validate our approach. Functional assays are being performed to confirm the involvement of Synapsin-I in the mechanisms underlying childhood absence epilepsy.
ISSN: 1535-3893

hal-00877728
https://hal.archives-ouvertes.fr/hal-00877728
DOI : 10.1021/pr3006974
PUBMED : 22994238

Éditeur(s) : HAL CCSD Ecological Society of America
Résumé : We investigated the relationship between habitat association and physiological performance in four congeneric species pairs exhibiting contrasting distributions between seasonally flooded and terra firme habitats in lowland tropical rain forests of French Guiana, including Virola and Iryanthera (Myristicaceae), Symphonia (Clusiaceae), and Eperua (Caesalpiniaceae). We analyzed 10-year data sets of mapped and measured saplings (stems 150 cm in height and <10 cm diameter at breast height [dbh]) and trees (stems 10 cm dbh) across 37.5 ha of permanent plots covering a 300-ha zone, within which seasonally flooded areas (where the water table never descends below 1 m) have been mapped. Additionally, we tested the response of growth, survival, and leaf functional traits of these species to drought and flood stress in a controlled experiment. We tested for habitat preference using a modification of the torus translation method. Strong contrasting associations of the species pairs of Iryanthera, Virola, and Symphonia were observed at the sapling stage, and these associations strengthened for the tree stage. Neither species of Eperua was significantly associated with flooded habitats at the sapling stage, but E. falcata was significantly and positively associated with flooded forests at the tree stage, and trees of E. grandiflora were found almost exclusively in nonflooded habitats. Differential performance provided limited explanatory support for the observed habitat associations, with only congeners of Iryanthera exhibiting divergent sapling survival and tree growth. Seedlings of species associated with flooded forest tended to have higher photosynthetic capacity than their congeners at field capacity. In addition, they tended to have the largest reductions in leaf gas exchange and growth rate in response to experimental drought stress and the least reductions in response to experimental inundation. The corroboration of habitat association with differences in functional traits and, to a lesser extent, measures of performance provides an explanation for the regional coexistence of these species pairs. We suggest that specialization to seasonally flooded habitats may explain patterns of adaptive radiation in many tropical tree genera and thereby provide a substantial contribution to regional tree diversity.
ISSN: 0012-9658

hal-01031746
https://hal.archives-ouvertes.fr/hal-01031746

Éditeur(s) : HAL CCSD Springer Verlag
Résumé : International audience
Nanotribological properties of NbSe2 are studied using an atomic friction force microscope. The friction force is measured as a function of normal load and scan speeds ranging from 10 nm s−1 to 40 μm s−1 under two atmospheres (air and argon). At low speed, no effect of atmosphere is noticed and a linear relationship between the friction and normal forces is observed leading to a friction coefficient close to 0.02 for both atmospheres. At high speed, the tip/surface contact obeys the JKR theory and the tribological properties are atmosphere dependent: the shear stress measured in air environment is three times lower than the one measured under argon atmosphere. A special attention is paid to interpret these results through numerical data obtained from a simple athermal model based on Tomlinson approach.
ISSN: 1023-8883

hal-00780496
https://hal.archives-ouvertes.fr/hal-00780496
https://hal.archives-ouvertes.fr/hal-00780496/document
https://hal.archives-ouvertes.fr/hal-00780496/file/Delbe_6345.pdf
DOI : 10.1007/s11249-008-9400-8

Éditeur(s) : HAL CCSD Oxford University Press (OUP)
Résumé : International audience
STUDY QUESTION: Do mild analgesics affect the endocrine system of the human adult testis? SUMMARY ANSWER: Mild analgesics induce multiple endocrine disturbances in the human adult testis in vitro. WHAT IS KNOWN ALREADY: Mild analgesics have recently been incriminated as potential endocrine disruptors. Studies of the effects of these widely used molecules on the androgenic status of men are limited and somewhat contradictory. This prompted us to investigate whether these compounds could alter the adult human testicular function. We therefore assessed in parallel the effects of paracetamol, aspirin and indomethacin on organo-cultured adult human testis and on the NCI-H295R steroid-producing human cell line. STUDY DESIGN, SIZE, DURATION: Adult human testis explants or NCI-H295R adrenocortical human cells were cultured with 10(-4) or 10(-5) M paracetamol, aspirin or indomethacin for 24-48 h. The effect of 10(-5) M ketoconazole, used as an anti-androgenic reference molecule, was also assessed. PARTICIPANTS/MATERIALS, SETTING, METHODS: Testes were obtained from prostate cancer patients, who had not received any hormone therapy. The protocol was approved by the local ethics committee of Rennes, France and informed consent was given by the donors. Only testes displaying spermatogenesis, as assessed by transillumination, were used in this study. Hormone levels in the culture media were determined by radioimmunoassay (testosterone, insulin-like factor 3), Enzyme-Linked Immunosorbent Assay (inhibin B) or Enzyme Immunosorbent Assay [prostaglandin (PG) D2, and PGE2]. Tissues were observed and cells counted using classical immunohistochemical methods. MAIN RESULTS AND THE ROLE OF CHANCE: The three mild analgesics caused multiple endocrine disturbances in the adult human testis. This was particularly apparent in the interstitial compartment. Effective doses were in the same range as those measured in blood plasma following standard analgesic treatment. The production of testosterone and insulin-like factor 3 by Leydig cells was altered by exposure to all these drugs. Inhibin B production by Sertoli cells was marginally affected by aspirin only. Our experiments also revealed that mild analgesics display direct anti-PG activity, which varied depending on the drug used, the dose and the duration of exposure. Nevertheless, associations between the alteration of the PG and testosterone profiles were not systematically observed, suggesting that a combination of mechanisms of endocrine disruption is at play. LIMITATIONS, REASONS FOR CAUTION: Our studies were performed in vitro. WIDER IMPLICATIONS OF THE FINDINGS: We provide the first evidence that direct exposure to mild analgesics can result in multiple endocrine disturbances in the human adult testis. Caution, concerning the consumption of mild analgesics by men, should be strengthened, particularly in high-risk population subgroups such as elite athletes.
ISSN: 0268-1161

inserm-00866016
http://www.hal.inserm.fr/inserm-00866016
PUBMED : 23670170
DOI : 10.1093/humrep/det112

Éditeur(s) : HAL CCSD European Geosciences Union
Résumé : We designed a thermo-mechanical numerical model for fast-spreading mid-ocean ridge with variable viscosity, hydrothermal cooling, latent heat release, sheeted dyke layer, and variable melt intrusion possibilities. The model allows for modulating several accretion possibilities such as the "gabbro glacier" (G), the "sheeted sills" (S) or the "mixed shallow and MTZ lenses" (M). These three crustal accretion modes have been explored assuming viscosity contrasts of 2 to 3 orders of magnitude between strong and weak phases and various hydrothermal cooling conditions depending on the cracking temperatures value. Mass conservation (stream-function), momentum (vorticity) and temperature equations are solved in 2-D cartesian geometry using 2-D, alternate direction, implicit and semi-implicit finite-difference scheme. In a first step, an Eulerian approach is used solving iteratively the motion and temperature equations until reaching steady states. With this procedure, the temperature patterns and motions that are obtained for the various crustal intrusion modes and hydrothermal cooling hypotheses display significant differences near the mid-ocean ridge axis. In a second step, a Lagrangian approach is used, recording the thermal histories and cooling rates of tracers travelling from the ridge axis to their final emplacements in the crust far from the mid-ocean ridge axis. The results show that the tracer's thermal histories are depending on the temperature patterns and the crustal accretion modes near the mid-ocean ridge axis. The instantaneous cooling rates obtained from these thermal histories betray these discrepancies and might therefore be used to characterize the crustal accretion mode at the ridge axis. These deciphering effects are even more pronounced if we consider the average cooling rates occurring over a prescribed temperature range. Two situations were tested at 1275-1125 °C and 1050-850 °C. The first temperature range covers mainly the crystallization range that is characteristic of the high temperature areas in the model (i.e. the near-mid-oceanic-ridge axis). The second temperature range corresponds to areas in the model where the motion is mainly laminar and the vertical temperature profiles are closer to conductive. Thus, this situation results in less discriminating efficiency among the crustal accretion modes since the thermal and dynamic properties that are described are common to all the crustal accretion modes far from the ridge axis. The results show that numerical modeling of thermo-mechanical properties of the lower crusts may bring useful information to characterize the ridge accretion structure, hydrothermal cooling and thermal state at the fast-spreading ridges and may open discussions with petrological cooling rate results.
ISSN: 1991-959X

hal-00907012
https://hal.archives-ouvertes.fr/hal-00907012
DOI : 10.5194/gmd-6-1659-2013

Éditeur(s) : HAL CCSD
Résumé : International audience
The corrosion rates in the presence of some indole derivates, namely, 9H-pyrido[3,4-b]indole (norharmane) and 1-methyl-9H-pyrido[3,4-b]indole (harmane), as inhibitors of C38 steel corrosion inhibitor in 1 M HCl solution, were measured by potentiodynamic polarization and electrochemical impedance spectroscopy (EIS) techniques, in the range of temperatures from 25 to 55∘C. Results obtained revealed that the organic compounds investigated have inhibiting properties for all temperatures. The inhibition was assumed to occur via adsorption of the indole molecules on the metal surface. Adsorption of indole derivates was found to follow the Langmuir isotherm. The apparent activation energies, enthalpies, and entropies of the dissolution process and the free energies and enthalpies for the adsorption process were determined by potentiodynamic polarization and electrochemical impedance. The fundamental thermodynamic functions were used to collect important information about indole inhibitory behaviour.
international Journal of Corrosion

hal-01683120
https://hal.univ-antilles.fr/hal-01683120
DOI : 10.1155/2013/139798

Éditeur(s) : HAL CCSD Elsevier
Résumé : International audience
Mesoporous cobalt oxide silica matrices and membranes were successfully prepared using a dual acid-base catalysed sol-gel and interlayer-free approach for desalination applications. The effects of cobalt addition (0-35 mol%) on the structure-property relationship of the silica materials and membrane performance were systematically studied as a function of feed salt concentration (0.3-7.5 wt.% NaCl) and temperature (22-60 °C). As cobalt concentration increases beyond 5 mol%, the concentration of tricobalt tetroxide and proportion of silica mesopores were found to increase within the matrices. Silica membranes with high amount of tricobalt tetroxide (35 mol%) were found to be highly mesoporous with water fluxes of 20, 11.3 and 7.7 kg m-2 h-1 for the 0.3, 3.5 and 7.5 wt.% solutions at 60 °C, respectively, with >99.7% salt rejection at all times. This is attributed to the high pore volume (mesopores formation) and structural robustness of the silica network provided by the presence of tricobalt tetroxide. Even higher performance was achieved for low tricobalt tetroxide (mol%) silica membranes with water fluxes of 47.6 (0.3 wt.%) and 31.5 (3.5 wt.%) kg m-2 h-1 at 60 °C, respectively, though with reduced salt rejections of 90%. © 2015 Elsevier B.V.
ISSN: 0011-9164

hal-01683209
https://hal.univ-antilles.fr/hal-01683209
DOI : 10.1016/j.desal.2015.02.034

Éditeur(s) : HAL CCSD De Gruyter
Résumé : International audience
A comparative study of 9H-pyrido[3,4-b]indole (norharmane) and 1-methyl-9H-pyrido[3,4-b]indole (harmane) as inhibitors for C38 steel corrosion in 1 M HCl solution at 25 °C was carried out. Potentiodynamic polarization and electrochemical impedance spectroscopy (EIS) techniques were applied to study the metal corrosion behavior in the absence and presence of different concentrations of these inhibitors. The OCP as a function of time were also established. Cathodic and anodic polarization curves show that norharmane and harmane are a mixed-type inhibitors. Adsorption of indole derivatives on the C38 steel surface, in 1 M HCl solution, follows the Langmuir adsorption isotherm model. The ΔGads values were calculated and discussed. The potential of zero charge (PZC) of the C38 steel in inhibited solution was studied by the EIS method, and a mechanism for the adsorption process was proposed. Raman spectroscopy confirmed that indole molecules strongly adsorbed onto the steel surface. The electronic properties of indole derivates, obtained using the AM1 semi-empirical quantum chemical approach, were correlated with their experimental efficiencies using the linear resistance model (LR).
ISSN: 0018-3830

hal-01093227
https://hal-agroparistech.archives-ouvertes.fr/hal-01093227
DOI : 10.1515/HF.2010.034

Éditeur(s) : HAL CCSD American Geophysical Union
Résumé : International audience
Seismic anisotropy has been widely studied this last decade, particularly by measuring splitting of vertically propagating core shear waves. The main interest in this technique is to characterize upper mantle flow beneath seismic stations. On the other hand, the major restriction in this method is that a single station gives a single anisotropy measurement. Alternative methods have been developed in order to avoid this restriction. An accurate determination of upper mantle seismic anisotropy beneath a seismic station may allow one, by doing anisotropy correction, to characterize remote or deeper anisotropy. The Geoscope network is ideal for this purpose because it is composed of a large set (about 26) of high-quality, broadband seismometers globally distributed and because some of these stations have run for more than 10 years and most of them for more than 5 years. We selected about 100 events at each site, generally of magnitude (m b) > 6.0, and we performed systematic measurements of the splitting parameters (fast polarization direction φ and delay time δt) on SKS, SKKS, and PKS phases. Splitting on oceanic islands has been difficult to observe owing to the low quality of the signal but also perhaps owing to complex upper mantle structures beneath the stations. Station KIP (Kipapa, Hawaii) in the Pacific is the only oceanic Geoscope station with a clear anisotropy. We determined well-constrained splitting parameters for 10 of the 17 continental stations that may be explained by a single anisotropic layer. The poor correlation between fast polarization directions and the absolute plate motion together with the apparent incoherence between the plate velocities and the observed delay times suggest that a simple drag-induced asthenospheric flow alone fails to explain most of the observations. For some stations located on or near major lithospheric structures (TAM, Tamanrasset, Algeria, for instance), we observe a good correlation between fast polarization directions and regional structures. At station SCZ (Santa Cruz, California), we found clear variations of the splitting parameters as a function of the event backazimuth, compatible with two layers of anisotropy. Three stations (CAN (Canberra), HYB (Hyderabad, India) and SSB (Saint Sauveur Badole, France)) seem to be devoid of detectable anisotropy.
ISSN: 2169-9313

hal-01388830
http://hal.univ-reunion.fr/hal-01388830
http://hal.univ-reunion.fr/hal-01388830/document
http://hal.univ-reunion.fr/hal-01388830/file/Barruol_anisoGeoscope_JGR1999.pdf
DOI : 10.1029/1999JB900033

Éditeur(s) : HAL CCSD Elsevier
Résumé : International audience
This work shows the preparation and separation performance assessment of carbon membranes derived from phenolic resin by a vacuum-assisted method and carbonisation in an inert atmosphere. The vacuum time played an important role in tailoring the structure of the membranes. For instance, pore volumes and surface areas increased from 0.81 and 834 to 2.2 cm3g−1and 1910 m2g−1, respectively, as the vacuum time exposure increased from 0 to 1200 s. The significant structural changes correlated very well with water permeation, as fluxes increased by 91% as the vacuum time increased from 0 to 1200 s reaching up to 169 L m−2h−1at 5 bar. Molecular weight cut-off tests showed no rejection for the smaller glucose and sucrose molecules, though this increased to ~ 80% and full rejection for 36 kDa and 400 kDa polyvinyl pyrrolidine. Interestingly, FTIR spectra showed that the peaks of C–H stretching vibration (2800–3200 cm−1) and C–O stretching (1030 cm−1) became more pronounced as a function of increasing vacuum time, strongly suggesting that the use of vacuum further assisted in the polycondensation of phenolic oligomers. Based on these outcomes, a cluster to cluster model is proposed, whereby vacuum application promoted crosslinking reactions of the phenolic resin, creating microporous regions within the clusters, and mesoporous regions between the clusters. © 2016 Elsevier B.V
ISSN: 0376-7388

hal-01683220
https://hal.univ-antilles.fr/hal-01683220
DOI : 10.1016/j.memsci.2016.11.002

Éditeur(s) : HAL CCSD Geological Society of London
Résumé : International audience
This paper presents the background for the calculation of various numbers that can be used to characterize crystal-preferred orientation (CPO), also known as texture in materials science, for large datasets using the combined scripting possibilities of MTEX and MatLab®. The paper is focused on three aspects in particular: the strength of CPO represented by orientation and misorientation distribution functions (ODFs, MDFs) or pole figures (PFs); symmetry of PFs and components of ODFs; and elastic tensors. The traditional measurements of texture strength of ODFs, MDFs and PFs are integral measurements of the distribution squared. The M-index is a partial measure of the MDF as the difference between uniform and measured misorientation angles. In addition there other parameters based on eigen analysis, but there are restrictions on their use. Eigen analysis does provide some shape factors for the distributions. The maxima of an ODF provides information on the modes. MTEX provides an estimate of the lower bound uniform fraction of an ODF. Finally, we illustrate the decomposition of arbitrary elastic tensor into symmetry components as an example of components in anisotropic physical properties. Ten examples scripts and their output are provided in the appendix.
Geological Society Special Publication

hal-01277689
https://hal.archives-ouvertes.fr/hal-01277689
DOI : 10.1144/SP409.8

Éditeur(s) : HAL CCSD Endocrine Society
Résumé : International audience
: Context:Masculinization depends on the fetal testis. Exposure of the human fetus during pregnancy to paracetamol or/and to other mild-analgesics is associated with an increased risk of cryptorchidism.Objective:We aimed at determining whether mild analgesics disrupted the morphology and endocrine function of the human testis.Design:We used an in vitro system based on the culture of human fetal testes exposed or not to paracetamol, its metabolite AM404, aspirin, indomethacin, ketoconazole at 10(-4) M to 10(-7) M.Setting:The study was conducted at the University of Rennes I.Patients/Participants:Human fetal testes were from pregnant women following induced abortion, between 7 and 12 weeks of gestation (GW).Main Outcome Measures:Testosterone (radioimmunoassay: RIA), Anti-Müllerian Hormone (AMH; ELISA), insulin-like factor 3 (INSL3; RIA), prostaglandin D2 and E2 (PGD2 and PGE2, respectively; ELISA), were assayed in the media. Testicular cells were counted using histology and image analysis. The possible nuclear receptor-mediated activities of the analgesics were investigated using reporter cell lines expressing estrogen (ERs), androgen (AR) and peroxysome proliferator-activated γ ( PPARγ) receptors.Results:Indomethacin and aspirin stimulated testosterone production, particularly by the younger testes (8-9 GW vs 10-12 GW). Paracetamol, AM404 and ketoconazole decreased INSL3 levels. Aspirin stimulated, whereas ketoconazole inhibited AMH production. PGE2 levels were inhibited by paracetamol, and aspirin in the 7-12 GW testes, and by indomethacin but only in 7-9.86 GW old testes. The inhibitory trends seen for PGD2 were not statistically significant.Conclusions:Analgesics at concentrations relevant to human exposure cause endocrine disturbances in the fetal testis. We suggest that the fetal human testis displays slight critical age windows for sensitivity to direct exposure to aspirin, indomethacin and paracetamol. The analgesic-induced inhibition of INSL3 may be the mechanism by which analgesics increase the risk of cryptorchidism. Greater caution is required concerning consumption of analgesics during pregnancy.
ISSN: 0021-972X

inserm-00876125
http://www.hal.inserm.fr/inserm-00876125
DOI : 10.1210/jc.2013-2531
PUBMED : 24030937

Éditeur(s) : HAL CCSD Oxford University Press (OUP)
Résumé : International audience
The Ronda peridotite massif in Southern Spain shows a well-defined 'recrystallization' front that separates a large-scale partial melting domain formed at the expense of the continental lithospheric mantle from a 'preserved' lithospheric domain. To investigate the processes allowing a transient lithosphere-asthenosphere boundary to propagate in the lithospheric mantle, we performed a joint petrostructural and geochemical study of an similar to 2.5 km(2) zone extending from the melting front to the mylonites that mark the western limit of the massif. This study emphasizes a feedback between heat transfer, melt percolation, and deformation in the lithospheric mantle. Petrographical observations and geochemical data show that heterogeneous reactive percolation of melts produced in the underlying partial melting domain led to refertilization of lithospheric peridotites up to 1.5 km ahead from the melting front, producing metre-scale layering of fertile and refractory mantle rocks. Within 800 m from the front, pre-existing garnet pyroxenite layers were partially molten and the resulting melts probably contributed to the refertilization process. Detailed structural mapping and analysis of the microstructures and crystal preferred orientations highlight the relations between reactive melt transport and deformation, and the control of the temperature gradient on both processes. Parallelism between the recrystallization front, compositional boundaries, and deformation structures, as well as variations in the deformation intensity of pyroxenes and spinels, suggest syn- to late-tectonic melt transport controlled by both the deformation and the thermal gradient. Variations in the strength of olivine crystal preferred orientations as a function of the modal and chemical composition of the spinel tectonites point to a higher contribution of diffusion to deformation in the most fertile rocks, corroborating the hypothesis that deformation occurred in presence of melt. Finally, the systematic dispersion of olivine [100] and orthopyroxene [001] axes in the foliation plane suggests a dominantly transpressive deformation regime.
ISSN: 0022-3530

hal-00420904
https://hal.archives-ouvertes.fr/hal-00420904
DOI : 10.1093/petrology/egp032

Éditeur(s) : HAL CCSD
Résumé : In spatial statistics, Ripley's K function (Ripley, 1977) is a classical tool to analyse spatial point patterns. Yet, it faces two major limits: it is only pertinent for homogeneous point processes and it does not allow the weighting of points.
We generalize it to get a new function, M, which oversteps these limits and detects spatial structures of inhomogeneous populations of weighted points.
https://halshs.archives-ouvertes.fr/halshs-00372631

halshs-00372631
https://halshs.archives-ouvertes.fr/halshs-00372631
https://halshs.archives-ouvertes.fr/halshs-00372631/document
https://halshs.archives-ouvertes.fr/halshs-00372631/file/HAL_Marcon_Puech_-_GeneralizingRipleysKFunctiontoInhomogeneousPopulations.pdf

Éditeur(s) : HAL CCSD
Résumé :  
In tropical rainforests, the origin of the interspecific variability in trunk CO2 efflux (Et) and of seasonal variations in Et have not yet been explained. We conducted a study to test which biotic and abiotic determinants would contribute to these variations. Et and a set of wood anatomical and functional characteristics were measured on trees in two habitats (Terra Firme forest vs. seasonal flooded forest) in a Neotropical rainforest over a 18-months' period including a range of climatic and soil water conditions. The large variability in Et (0.33 to 3.15 μmol CO2 m-2 s-1 in Terra Firme forest) was associated with tree diameter and growth respiration (Eg), although maintenance respiration (Em) was the main component of Et. Neither wood properties nor sapflow density were correlated with trunk CO2 efflux. The large seasonal variations in Et were correlated with diameter growth and surface soil water content (SWC). These data underlined the absence of clearly identified biotic determinants, beside growth, to explain the interspecific variability in Et. We emphasized the fast response of growth and maintenance respiration processes to seasonal variations in SWC that potentially has consequences on the carbon cycle in the ecosystem under upcoming climatic changes.
23. New Phytologist Symposium. Carbon cycling in tropical ecosystems
Guangzhou, China

hal-01032199
https://hal.archives-ouvertes.fr/hal-01032199

Éditeur(s) : HAL CCSD American Society for Microbiology
Résumé : International audience
We investigated the characteristics of the sulfur-oxidizing symbiont hosted in the gills of Codakia orbicularis, a bivalve living in shallow marine tropical environments. Special attention was paid to describing the heterogeneity of the population by using single-cell approaches including flow cytometry (FCM) and different microscopic techniques and by analyzing a cell size fractionation experiment. Up to seven different subpopulations were distinguished by FCM based on nucleic acid content and light side scattering of the cells. The cell size analysis of symbionts showed that the symbiotic population was very heterogeneous in size, i.e., ranging from 0.5 to 5 _m in length, with variable amounts of intracellular sulfur. The side-scatter signal analyzed by FCM, which is often taken as a proxy of cell size, was greatly influenced by the sulfur content of the symbionts. FCM revealed an important heterogeneity in the relative nucleic acid content among the subclasses. The larger cells contained exceptionally high levels of nucleic acids, suggesting that these cells contained multiple copies of their genome, i.e., ranging from one copy for the smaller cells to more than four copies for the larger cells. The proportion of respiring symbionts (5-cyano-2,3-ditolyl-terazolium chloride positive) in the bacteriocytes of Codakia revealed that around 80% of the symbionts hosted by Codakia maintain respiratory activity throughout the year. These data allowed us to gain insight into the functioning of the symbionts within the host and to propose some hypotheses on how the growth of the symbionts is controlled by the host.
ISSN: 0099-2240

hal-00716079
https://hal.univ-antilles.fr/hal-00716079
DOI : 10.1128/AEM.01683-06

Éditeur(s) : HAL CCSD Sociedade Entomológica do Brasil
Résumé : Variations in diel activity among hyperdiverse tropical communities of moths, despite representing a key component of niche partitioning between species, have barely been studied so far. Using light trapping from dawn to sunset over a 1-year period in French Guiana, we investigated these variations within and between two families of moths (Sphingidae and Saturniidae). Our results revealed contrasting patterns in flight activity at night between Sphingidae and Saturniidae. Sphingidae reached their peak in species richness and abundance between 7:00 and 8:00 p.m., followed by a decrease around 11:00 p.m. to midnight, whereas Saturniidae were continuously present throughout the night, with a peak around midnight. In addition, we found changes in diel activity among some of the most common genera in each family, highlighting distinct behavioral, physiological, and functional traits among taxa. Given differences in flight activity at different taxonomic levels, it is strongly recommended to monitor by light trapping throughout the night to effectively sample saturniid and sphingid assemblages, even though the activity of Sphingidae sharply declines after midnight. These results improve the general natural history information of tropical moths and reinforce the need of further research on the ecological and taxonomic consequences of differences in diel activity.
ISSN: 1519-566X

hal-01536447
https://hal.archives-ouvertes.fr/hal-01536447
DOI : 10.1007/s13744-014-0264-3
PRODINRA : 320714

Éditeur(s) : HAL CCSD American Physiological Society
Résumé : International audience
Interleukin (IL)-33 is crucially involved in liver pathology and drives hepatoprotective functions. However, the regulation of IL-33 by cytokines of the IL-6 family, including oncostatin M (OSM) and IL-6, is not well studied. The aim of the present study was to determine whether OSM mediates regulation of IL-33 expression in liver cells. Intramuscular administration in mice of an adenovirus encoding OSM (AdOSM) leads to increase in expression of OSM in muscles, liver, and serum of AdOSM-infected mice compared with control mice. The increase of circulating OSM markedly regulated mRNA of genes associated with blood vessel biology, chemotaxis, cellular death, induction of cell adhesion molecules, and the alarmin cytokine IL-33 in liver. Steady-state IL-33 mRNA was upregulated by OSM at an early phase (8 h) following AdOSM infection. At the protein level, the expression of IL-33 was significantly induced in liver endothelial cells [liver sinusoidal endothelial cells (LSEC) and vascular endothelial cells] with a peak at 8 days post-AdOSM infection in mice. In addition, we found OSM-stimulated human microvascular endothelial HMEC-1 cells and human LSEC/TRP3 cells showed a significant increase in expression of IL-33 mRNA in a dose-dependent manner in cell culture. The OSM-mediated overexpression of IL-33 was associated with the activation/enrichment of CD4(+)ST2(+) cells in liver of AdOSM-infected mice compared with adenovirus encoding green fluorescent protein-treated control mice. In summary, these data suggest that the cytokine OSM regulates the IL-33 expression in liver endothelial cells in vivo and in HMEC-1/TRP3 cells in vitro and may specifically expand the target CD4(+)ST2(+) cells in liver
ISSN: 0193-1857

hal-01220632
https://hal-univ-rennes1.archives-ouvertes.fr/hal-01220632
DOI : 10.1152/ajpgi.00398.2014
PUBMED : 26251474