Éditeur(s) : HAL CCSD Oxford University Press (OUP)
Résumé : International audience
To improve earthquake location, we create a 3-D a priori P-wave velocity model (3-DVM) that approximates the large velocity variations of the Ecuadorian subduction system. The 3-DVM is constructed from the integration of geophysical and geological data that depend on the structural geometry and velocity properties of the crust and the upper mantle. In addition, specific station selection is carried out to compensate for the high station density on the Andean Chain. 3-D synthetic experiments are then designed to evaluate the network capacity to recover the event position using only P arrivals and the MAXI technique. Three synthetic earthquake location experiments are proposed: (1) noise-free and (2) noisy arrivals used in the 3-DVM, and (3) noise-free arrivals used in a 1-DVM. Synthetic results indicate that, under the best conditions (exact arrival data set and 3-DVM), the spatiotemporal configuration of the Ecuadorian network can accurately locate 70 per cent of events in the frontal part of the subduction zone (average azimuthal gap is 289° ± 44°). Noisy P arrivals (up to ± 0.3 s) can accurately located 50 per cent of earthquakes. Processing earthquake location within a 1-DVM almost never allows accurate hypocentre position for offshore earthquakes (15 per cent), which highlights the role of using a 3-DVM in subduction zone. For the application to real data, the seismicity distribution from the 3-D-MAXI catalogue is also compared to the determinations obtained in a 1-D-layered VM. In addition to good-quality location uncertainties, the clustering and the depth distribution confirm the 3-D-MAXI catalogue reliability. The pattern of the seismicity distribution (a 13 yr record during the inter-seismic period of the seismic cycle) is compared to the pattern of rupture zone and asperity of the Mw = 7.9 1942 and the Mw = 7.7 1958 events (the Mw = 8.8 1906 asperity patch is not defined). We observe that the nucleation of 1942, 1958 and 1906 events coincides with areas of positive Simple Bouguer anomalies and areas where marine terraces are still preserved on the coastal morphology. From north to south: (1) the 1958 rupture zone is almost aseismic and is attributed to a zone of high coupling; (2) south of the Galera alignment (perpendicular to the trench), the 1942 rupture zone presents moderate seismicity, deeper on the seismogenic interplate zone, and abutting on the Jama cluster (to the south). This cluster is facing the Cabo Pasado cap and positive Bouguer anomalies on the overriding margin. We suspect that this cluster reflects a zone of local asperity (partial coupling). South of the Jama cluster, the spherical aseismic zone in the Bahia area is interpreted as having a low seismic coupling (steady creep motion or slow slip events). We suspect that the site that generated the three M > 7 events (1896, 1956 and 1998) correspond to a small patch of strong coupling. To the south, in the Manta-Puerto Lopez zone, the seismicity is mainly organized in earthquake swarms (1998, 2002, 2005). Although slow slip events have been observed in the area (Vallée et al. submitted), we infer from the coastline shape, the marine terraces and the high positive Bouguer anomalies that the seismicity here might reveal a significant amount of seismic coupling.
ISSN: 0956-540X

hal-00873478
https://hal.archives-ouvertes.fr/hal-00873478
DOI : 10.1093/gji/ggs083

Éditeur(s) : HAL CCSD Elsevier
Résumé : International audience
Volumetrically minor microsyenites, alkali microgranite and related trachytic dykes intrude early Pliocene OIB-like alkali basaltic and basanitic flows of the Meseta del Lago Buenos Aires in Central Patagonia (47 degrees S-71 degrees 30'W), and occur together with scarce trachytic lava flows. Whole-rock K-Ar ages between 3.98 and 3.08 Ma indicate that the emplacement of these felsic rocks occurred more or less synchronously with that of the post-plateau basaltic sequence that they intrude, during a bimodal mafic-felsic magmatic episode devoid of intermediate compositions. Chemically, these rocks have A(1)-type granitoid affinities and are characterized by high silica and alkali contents (60-68 wt.% SiO2; 8.7-10.8 wt.% Na2O+K2O), major and trace elements patterns evidencing evolution by low-pressure fractional crystallization, and Sr and Nd isotopic signatures similar to those of coeval basalts ((Sr-87/Sr-86)o=0.70488-0.70571; (Nd-143/Nd-144)(o)=0.512603-0.512645). Nevertheless, some of them have the most radiogenic Sr values ever reported for a magmatic rock in the Meseta and even in the whole Neogene Patagonian Plateau Lavas province ((Sr-17/Sr-86)(o)= 0.70556-0.70571; (Nd-143/Nd-144)(o)=0.512603-0.512608). In addition, very high contents of strongly incompatible elements in the most evolved rocks, together with Sr isotopic ratios higher than those of coeval basalts, suggest the occurrence of open-system magmatic processes. Continuous fractional crystallization from a primitive basaltic source, similar to post-plateau coeval basalts, towards alkali granites combined with small rates of assimilation of host Jurassic tuffs (AFC) in a shallow magmatic reservoir, best explains the geochemical and petrographic features of the felsic rocks. Therefore, A(1)-type magmatic rocks can be generated by open-system crystallization of deep asthenospheric melts in back-are tectonic settings. In Central Patagonia, these similar to 3-4 Ma old alkaline intrusions occur aligned along a similar to N160-170 trending lineament, the Zeballos Fault Zone, stacking the morphotectonic front of one segment of the Patagonian Cordillera. Intrusion along this fault zone
ISSN: 0024-4937

hal-00412030
https://hal.archives-ouvertes.fr/hal-00412030
DOI : 10.1016/j.lithos.2007.07.002

Éditeur(s) : HAL CCSD Elsevier
Résumé : International audience
In this contribution we present a reconstruction of the overall lithotectonic architecture, from inner zones to external ones, of the Paleoproterozoic Trans North China Orogen, within the North China Craton. Moreover, forward thermobarometrical modeling on a kyanite-bearing gneiss yields a reliable prograde P-T-t-D path. In addition, 40Ar/39Ar dating on rocks from distinct litho-tectonic units helps us to distinguish several tectono-metamorphic events during the orogenic development. Considering these results, we propose a geodynamic model involving three cratonic blocks, namely the Western, Fuping and Eastern Blocks, separated by two oceans, the Lüliang and Taihang Oceans. The opening of oceanic basins occurred around 2.2-2.3 Ga. After the westward subductions of oceanic lithosphere, the Trans-North-China Orogen was built up through a polyphase tectonic evolution within the period 1900-1800 Ma. The first event (D1) corresponded to the emplacement of lower and upper nappes herein called the Orthogneiss-and-Volcanites Unit (OVU) and the Low-Grade-and-Mafic Unit (LGMU), respectively. The syn-metamorphic D1 deformation (1880 ± 10 Ma) is characterized by a NW-SE stretching and mineral lineation with a top-to-the SE sense of shear. During ongoing compression of the thickening orogenic crust, a second deformation event D2 (1850 ± 10 Ma) was responsible for (1) syn-anatectic lateral flow and exhumation of the orogenic root and (2) folding of the middle and upper parts of the orogenic wedge that consequently acquired a fan-type geometry. The late D3 (1830 ± 10 Ma) and D4 (1810 ± 10 Ma) events are related to late-orogenic normal and strike-slip shearing, respectively. In our present state of knowledge, the Paleoproterozoic Trans-North China Orogen might be regarded as the assemblage of two continent-continent collisional belts, both of which are characterized by nappe stacking accommodated by top-to-the E/SE ductile shearing. Continental subduction, crustal thickening, partial melting of overthickened crust, exhumation of HP rocks and deposition of syn-orogenic detrital basins are typical features of modern collisional-type orogens.
ISSN: 0301-9268

Droits : info:eu-repo/semantics/OpenAccess
insu-00628119
https://hal-insu.archives-ouvertes.fr/insu-00628119
https://hal-insu.archives-ouvertes.fr/insu-00628119/document
https://hal-insu.archives-ouvertes.fr/insu-00628119/file/Trap-PrecambrianResearch-2011.pdf
DOI : 10.1016/j.precamres.2011.09.008

Éditeur(s) : HAL CCSD
Résumé : International audience
The Aegean domain is a key area for understanding the processes of back-arc extension. Observed deformation pattern and present day kinematics result from the interaction between the southward retreat of the Hellenic trench and the westward escape of Anatolia. Using laboratory experiments designed to study lithosphere-scale deformation, we show that the overall pattern of Aegean extension requires not only the combination of trench retreat and Anatolia escape since 15 Ma but also the presence of an inherited lithosphere-scale mechanical discontinuity: the Vardar Suture Zone (VSZ). The reactivation in dextral shear of the eastern branch of the VSZ accommodates both the trench retreat (NS stretching) and the westward escape of Anatolia (EW shortening) in the Cyclades area since 15 Ma. Additionally, our model shows that the North Anatolian Fault (NAF) is a late structure in the evolution of the Aegean, initiated 10 Ma after the onset of Anatolia escape. The model displays displacement sub-domains, which result from strain partitioning instead of being “rigid microplates”, directly comparable to the present-day displacement field (GPS) of the Aegean and western Anatolia. Our modelling provides a simple way to look at the dynamics of Aegean extension in two main stages. From middle Eocene to middle Miocene, extension was only driven by the southward retreat of the Hellenic trench at a rate lower than 1 cm.y-1. Since middle Miocene, the combination of slab rollback with Anatolia westward escape, resulted in a southwest direction of trench retreat, with an accelerating rate of up to 3 cm.y-1.
AGU Fall Meeting 2015
San Francisco, United States

insu-01242045
https://hal-insu.archives-ouvertes.fr/insu-01242045

Éditeur(s) : HAL CCSD Public Library of Science
Résumé : International audience
Brain-derived neurotrophic factor (BDNF), a member of the neurotrophin family, has emerged as an active mediator in many essential functions in the central nervous system of mammals. BDNF plays significant roles in neurogenesis, neuronal maturation and/or synaptic plasticity and is involved in cognitive functions such as learning and memory. Despite the vast literature present in mammals, studies devoted to BDNF in the brain of other animal models are scarse. Zebrafish is a teleost fish widely known for developmental genetic studies and is emerging as model for translational neuroscience research. In addition, its brain shows many sites of adult neurogenesis allowing higher regenerative properties after traumatic injuries. To add further knowledge on neurotrophic factors in vertebrate brain models, we decided to determine the distribution of bdnf mRNAs in the larval and adult zebrafish brain and to characterize the phenotype of cells expressing bdnf mRNAs by means of double staining studies. Our results showed that bdnf mRNAs were widely expressed in the brain of 7 days old larvae and throughout the whole brain of mature female and male zebrafish. In adults, bdnf mRNAs were mainly observed in the dorsal telencephalon, preoptic area, dorsal thalamus, posterior tuberculum, hypothalamus, synencephalon, optic tectum and medulla oblongata. By combining immunohistochemistry with in situ hybridization, we showed that bdnf mRNAs were never expressed by radial glial cells or proliferating cells. By contrast, bdnf transcripts were expressed in cells with neuronal phenotype in all brain regions investigated. Our results provide the first demonstration that the brain of zebrafish expresses bdnf mRNAs in neurons and open new fields of research on the role of the BDNF factor in brain mechanisms in normal and brain repairs situations.
ISSN: 1932-6203

hal-01359562
https://hal-univ-rennes1.archives-ouvertes.fr/hal-01359562
DOI : 10.1371/journal.pone.0158057
PUBMED : 27336917

Éditeur(s) : HAL CCSD
Résumé : Climate change is one of the biggest public health threats in the 21th century. An increase in temperatures will lead to an increase in mortality attributable to temperature. In addition, some populations and territories are particularly vulnerable to the impact of increases in heat. It is thus necessary to identify these populations and territories as well as examine future heat-Related health impacts in order to recommend equity-Oriented policies today and in the future. The general objective of this thesis is to document current and future heat-Related vulnerability factors in the context of climate change. In order to address this general objective, the thesis involved four components: a) to conduct a systematic review and a meta-Analysis to assess the heterogeneity in the heat-Mortality associations with respect to individual and contextual population characteristics; b) to identify whether and how the magnitude of mean temperature effects on all-Cause mortality were modified by chronic air pollution exposure, social deprivation, and a combination of these two dimensions; c) to develop a method to quantify the climate change impacts on heat-Related mortality using climate modeling; d) to assess historical and future social disparities in years of life lost caused by ambient temperature in Montreal and Paris, and compare these estimates as well as the impact of climate change on social disparities between the two cities. This thesis highlights which populations are more vulnerable to heat and shows that several differences exist with regard to guidelines from international public health institutions for the identification of vulnerable populations. This thesis also identified chronic air pollution exposure as a new vulnerability factor in heat-Related mortality and that it has a double interaction with social deprivation. Furthermore, in this thesis a novel method to quantify future heat-Related mortality was developed which emphasized the strong evidence of an increase in heat-Related mortality under climate change. This method was then applied to estimate the increase in daily years of life lost social disparities in both Montreal and Paris under climate change which showed that this increase would be greater in Montreal compared to Paris in the future. Thus, this thesis which used a variety of epidemiologic methods has clarified which populations are particularly vulnerable to heat impacts and challenges guidelines for the identification of vulnerable populations from international public health institutions. It has also highlighted the climate change impacts on health inequalities and aims to reorient equity-Focused policies.
Les changements climatiques constituent l’un des enjeux les plus importants en santé publique du XXIème siècle. En effet, une hausse des températures provoquera une hausse de la mortalité attribuable à la chaleur. En outre, certaines populations et territoires sont particulièrement vulnérables aux effets de la chaleur. Il est donc nécessaire de les identifier clairement ainsi que les impacts futurs pour orienter avec équité les politiques publiques aujourd’hui et à l’avenir. L’objectif de cette thèse est de documenter les facteurs de vulnérabilité à la chaleur dans le présent et les éléments permettant leur prise en compte pour l’avenir dans le contexte des changements climatiques. Quatre étapes ont été menées pour répondre à cet objectif : a) Mener une revue systématique et une méta-Analyse des facteurs de vulnérabilité face aux risques de mortalité en lien avec la chaleur ; b) Analyser si l’exposition chronique à la pollution atmosphérique modifie la relation entre la chaleur et la mortalité dans un contexte urbain (Paris) et explorer la double interaction avec la défaveur sociale ; c) Développer une méthode de quantification des impacts liés à la chaleur en lien avec les changements climatiques en intégrant une grande diversité de simulations climatiques ; d) Estimer les inégalités d’années de vies perdues attribuables à la chaleur et leurs projections futures dans deux contextes distincts (Montréal et Paris) et comparer ces inégalités. Cette thèse a permis de mettre en évidence sur la base de la littérature épidémiologique les groupes de population qui sont les plus vulnérables face aux effets de la chaleur et de montrer qu’il y a plusieurs divergences par rapport aux recommandations émises par les institutions de santé publique vis-À-Vis de l’identification de populations vulnérables. Cette thèse a permis également d’identifier l’exposition chronique à la pollution atmosphérique comme nouveau facteur de vulnérabilité à la chaleur et que cette vulnérabilité était encore plus prononcée lorsqu’il s’agissait de populations défavorisées socialement. Puis, une méthode permettant de quantifier l’impact des changements climatiques sur les décès attribuables à la chaleur et ses sources d’incertitudes a été développée, et a permis de mettre en évidence que la probabilité que les changements climatiques conduisent à une augmentation de la mortalité en lien avec la chaleur est très forte. Cette méthode a ensuite été utilisée pour estimer que l’augmentation de la température conduira à une augmentation des inégalités sociales d’années de vie perdues à la fois à Montréal et à Paris, l’effet des changements climatiques sur l’accroissement de ces inégalités étant plus fort à Montréal qu’à Paris. Cette thèse, en se basant sur diverses méthodes épidémiologiques, a permis dans l’ensemble de clarifier quelles populations étaient particulièrement à risque face aux effets de la chaleur et de questionner les recommandations émises par les organismes tels que l’OMS. Elle a également permis de montrer l’effet des changements climatiques sur l’évolution de vulnérabilités face à la chaleur pour inciter dès aujourd’hui la mise en place de politiques publiques équitables et limiter l’impact des changements climatiques sur l’accroissement des inégalités de santé.
https://tel.archives-ouvertes.fr/tel-01456772

Droits : info:eu-repo/semantics/OpenAccess
NNT : 2015PA11T003
tel-01456772
https://tel.archives-ouvertes.fr/tel-01456772
https://tel.archives-ouvertes.fr/tel-01456772/document
https://tel.archives-ouvertes.fr/tel-01456772/file/VA_BENMARHNIA_TARIK_05022015.pdf

Éditeur(s) : HAL CCSD
Résumé : Biogenic volatile organic compound (BVOCs) emissions play an important role in regional air quality and global atmospheric chemistry. In addition, these natural VOC emissions serve important biological functions including attracting and repelling pollinators and herbivores. Some biological organisms use ambient air as a communication medium and the oxidation of these compounds brings about the concentration gradients sensed by insects and other organisms. Isoprene is the predominant BVOC emitted by vegetation and tropical forests are the dominant global source. This compound is very reactive in the atmosphere and contributes to the reactions that control tropospheric oxidant concentrations and thus the concentrations and lifetimes of longer-lived species. This paper presents a study on the seasonal variations in isoprene and some other significant BVOCs such as µ-pinene, µ-pinene, limonene, e-µ ocimene and longifolene, measured at the Guyaflux Tower located in a wet tropical forest in Paracou French Guiana (5o16´54´´N, 52o54´44´´W), during the year of 2011, using the Relaxed Eddy Accumulation technique at approximately 20 meters high above the canopy. The results show a lower concentration of isoprene during the month of February and March which correspond to the wet season with an average of 0,545 µg/m3 and 0,341 µg/m3, respectively followed by a slight increase in middle April (still wet season) and a higher concentration later in mid-June. The same behavior was observed for µ-pinene with higher concentrations for the same periods as isoprene however with a smaller increase. All the other compounds had concentrations below 1 µg/m3during the whole year: The monoterpene, e-µ ocimene, was observed and is known as a stress compound but the vegetation at the site did not face any known severe stress condition such as excessive drought or flooding. Concerning the fluxes, the results showed that just a small amount of BVOCs were deposited by wet or dry process and the majority of them were released in the atmosphere. Isoprene was by far the biogenic volatile organic compound with the highest concentration and flux, followed by alpha-pinene. The lowest concentration and flux rate for all the studied compounds was observed during the months of March and late July and beginning of August indicating a lower production of those BVOCs by vegetation during those periods. Previous limited studies in Amazonia and the Congo suggested that a higher concentration and flux rate of isoprene and µ-pinene should be expected during the dry season with lower emissions during the wet season, which is in relative agreement with what was observed at this tropical forest site in French Guiana. The exceptions were observed in April and June which correspond to a long wet period in which the concentration of isoprene and µ-pinene increased more than it was expected for this time of the year. The observations will be compared to output from the global chemistry transport model CAM-chem, which includes the MEGAN biogenic emissions model.
10th EGU General Assembly
vienne, Austria

hal-01204214
https://hal.archives-ouvertes.fr/hal-01204214
PRODINRA : 276525

Éditeur(s) : HAL CCSD Elsevier
Résumé : International audience
The thermal evolution of sediments in the shallowest part of sedimentary basins is challenging to investigate as most of the geothermometers are at their application limits. This is typically the case of the Callovian-Oxfordian claystones at the eastern border of the Paris Basin. A recent 2000 m deep well penetrated the entire Jurassic and Triassic sedimentary series and permitted coring of sediments never reached by previous drilling in this part of the basin. Thanks to the use of several independent geothermometers (apatite fission track thermochronology, fluid inclusion microthermometry, clay mineralogy and sedimentary organic matter evolution) the burial history of the Mesozoic series has been reconstructed, modeled and calibrated using kinetic algorithms relevant to the various paleothermometers used. In addition to these conventional methods, an innovative approach was developed using the temperature-dependence of the magnetic mineral assemblage within clay-rich sediments ("MagEval"). Thermal modeling indicates that the Callovian-Oxfordian and the Lower Triassic experienced maximum burial temperatures of 50 +/- 5 C-circle and 90 +/- 10 C-circle respectively, i.e., about 25 C higher than present-day. This temperature offset implies the erosion of several hundred meters of sediments which, depending on the thermal flux and conductivity considered, most likely constituted Upper Cretaceous chalk deposits. Falling sea level resulted in the exposure and subsequent dismantling of the chalk cover at the beginning of Cenozoic times. The eastern border of the basin was then subjected to slow erosion and discrete tectonic deformation.
ISSN: 0264-8172

hal-01313142
https://hal.archives-ouvertes.fr/hal-01313142
DOI : 10.1016/j.marpetgeo.2013.08.019

Éditeur(s) : HAL CCSD Elsevier
Résumé : International audience
Human hepatocytes cultured in a monolayer configuration represent a well-established in vitro model in liver toxicology, notably used in drug transporter studies. Polarized status of drug transporters, i.e., their coordinated location at sinusoidal or canalicular membranes, remains however incompletely documented in these cultured hepatocytes. The present study was therefore designed to analyze transporter expression and location in such cells. Most of drug transporters were first shown to be present at notable mRNA levels in monolayer-cultured human hepatocytes. Cultured human hepatocytes, which morphologically exhibited bile canaliculi-like structures, were next demonstrated, through immunofluorescence staining, to express the influx transporters organic anion transporting polypeptide (OATP) 1B1, OATP2B1 and organic cation transporter (OCT) 1 and the efflux transporter multidrug resistance-associated protein (MRP) 3 at their sinusoidal pole. In addition, the efflux transporters P-glycoprotein and MRP2 were detected at the canalicular pole of monolayer-cultured human hepatocytes. Moreover, canalicular secretion of reference substrates for the efflux transporters bile salt export pump, MRP2 and P-glycoprotein as well as sinusoidal drug transporter activities were observed. This polarized and functional expression of drug transporters in monolayer-cultured human hepatocytes highlights the interest of using this human in vitro cell model in xenobiotic transport studies.
ISSN: 0887-2333

hal-01141725
https://hal-univ-rennes1.archives-ouvertes.fr/hal-01141725
https://hal-univ-rennes1.archives-ouvertes.fr/hal-01141725/document
https://hal-univ-rennes1.archives-ouvertes.fr/hal-01141725/file/Polarized%20location%20of%20SLC.pdf
DOI : 10.1016/j.tiv.2015.03.019

Éditeur(s) : HAL CCSD Public Library of Science
Résumé : International audience
Among persisting questions on bone calcification, a major one is the link between protein expression and mineral deposition. A cell culture system is here proposed opening new integrative studies on biomineralization, improving our knowledge on the role played by non-collagenous proteins in bone. This experimental in vitro model consisted in human primary osteoblasts cultured for 60 days at the surface of a 3D collagen scaffold mimicking an osteoid matrix. Various techniques were used to analyze the results at the cellular and molecular level (adhesion and viability tests, histology and electron microscopy, RT-and qPCR) and to characterize the mineral phase (histological staining, EDX, ATG, SAED and RMN). On long term cultures human bone cells seeded on the osteoid-like matrix displayed a clear osteoblast phenotype as revealed by the osteoblast-like morphology, expression of specific protein such as alkaline phosphatase and expression of eight genes classically considered as osteoblast markers, including BGLAP, COL1A1, and BMP2. Von Kossa and alizarine red allowed us to identify divalent calcium ions at the surface of the matrix, EDX revealed the correct Ca/P ratio, and SAED showed the apatite crystal diffraction pattern. In addition RMN led to the conclusion that contaminant phases were absent and that the hydration state of the mineral was similar to fresh bone. A temporal correlation was established between quantified gene expression of DMP1 and IBSP, and the presence of hydroxyapatite, confirming the contribution of these proteins to the mineralization process. In parallel a difference was observed in the expression pattern of SPP1 and BGLAP, which questioned their attributed role in the literature. The present model opens new experimental possibilities to study spatio-temporal relations between bone cells, dense collagen scaffolds, NCPs and hydroxyapatite mineral deposition. It also emphasizes the importance of high collagen density environment in bone cell physiology.
ISSN: 1932-6203

hal-01142641
http://hal.upmc.fr/hal-01142641
http://hal.upmc.fr/hal-01142641/document
http://hal.upmc.fr/hal-01142641/file/Silvent_2013_Collagen.pdf
DOI : 10.1371/journal.pone.0057344

Éditeur(s) : HAL CCSD Wiley-Blackwell
Résumé : International audience
UNLABELLED: Interleukin (IL)-33, a member of the IL-1 cytokine family, positively correlates with acute hepatitis and chronic liver failure in mice and humans. IL-33 is expressed in hepatocytes and is regulated by natural killer T (NKT) cells during concanavalin A (ConA)-induced acute liver injury. Here, we investigated the molecular mechanisms underlying the expression of IL-33 during acute hepatitis. The expression of IL-33 and its regulation by death receptor pathways was investigated after the induction of ConA-acute hepatitis in wildtype (WT), perforin(-/-) , tumor necrosis factor related apoptosis inducing ligand (TRAIL)(-/-) , and NKT cell-deficient (CD1d(-/-) ) mice. In addition, we used a model of acute liver injury by administering Jo2/Fas-antibody or D-galactosamine-tumor necrosis factor alpha (TNFα) in WT mice. Finally, the effect of TRAIL on IL-33 expression was assessed in primary cultured murine hepatocytes. We show that IL-33 expression in hepatocytes is partially controlled by perforin during acute liver injury, but not by TNFα or Fas ligand (FasL). Interestingly, the expression of IL-33 in hepatocytes is blocked during ConA-acute hepatitis in TRAIL-deficient mice compared to WT mice. In contrast, administration of recombinant murine TRAIL associated with ConA-priming in CD1d-deficient mice or in vitro stimulation of murine hepatocytes by TRAIL but not by TNFα or Jo2 induced IL-33 expression in hepatocytes. The IL-33-deficient mice exhibited more severe ConA liver injury than WT controls, suggesting a protective effect of IL-33 in ConA-hepatitis. CONCLUSION: The expression of IL-33 during acute hepatitis is dependent on TRAIL, but not on FasL or TNFα.
ISSN: 0270-9139

hal-00874194
https://hal.archives-ouvertes.fr/hal-00874194
PUBMED : 22961755
DOI : 10.1002/hep.25893

Éditeur(s) : HAL CCSD Springer Verlag
Résumé : International audience
In ophiolites and in present-day oceanic crust formed at fast spreading ridges, oceanic plagiogranites are commonly observed at, or close to the base of the sheeted dike complex. They can be produced either by differentiation of mafic melts, or by hydrous partial melting of the hydrothermally altered sheeted dikes. In addition, the hydrothermally altered base of the sheeted dike complex, which is often infiltrated by plagiogranitic veins, is usually recrystallized into granoblastic dikes that are commonly interpreted as a result of prograde granulitic metamorphism. To test the anatectic origin of oceanic plagiogranites, we performed melting experiments on a natural hydrothermally altered dike, under conditions that match those prevailing at the base of the sheeted dike complex. All generated melts are water saturated, transitional between tholeiitic and calc-alkaline, and match the compositions of oceanic plagiogranites observed close to the base of the sheeted dike complex. Newly crystallized clinopyroxene and plagioclase have compositions that are characteristic of the same minerals in granoblastic dikes. Published silicic melt compositions obtained in classical MORB fractionation experiments also broadly match the compositions of oceanic plagiogranites; however, the compositions of the coexisting experimental minerals significantly deviate from those of the granoblastic dikes. Our results demonstrate that hydrous partial melting is a likely common process in the root zone of the sheeted dike complex, starting at temperatures exceeding 850A degrees C. The newly formed melt can either crystallize to form oceanic plagiogranites or may be recycled within the melt lens resulting in hybridized and contaminated MORB melts. It represents the main MORB crustal contamination process. The residue after the partial melting event is represented by the granoblastic dikes. Our results support a model with a dynamic melt lens that has the potential to trigger hydrous partial melting reactions in the previously hydrothermally altered sheeted dikes. A new thermometer using the Al content of clinopyroxene is also elaborated.
ISSN: 0010-7999

hal-00534058
https://hal.archives-ouvertes.fr/hal-00534058
DOI : 10.1007/s00410-010-0502-6

Éditeur(s) : HAL CCSD Elsevier
Résumé : We have studied the phase transformation of forsterite to wadsleyite under shear stress at the Earth's transition zone pressure and temperature conditions. Two-step experiments were performed using a multi-anvil press. First, we hot pressed iron-free forsterite at 6 or 11 GPa and 1100 degrees C. Then we deformed a slab of this starting material using a direct simple shear assembly at 16 GPa and 1400 degrees C for 1, 15, 35, 40, or 60 min. Both the starting material and the deformed samples were characterized using optical and scanning electron microscopy including measurements of crystal preferred orientations (CPO) by electron back scattered diffraction (EBSD), transmission electron microscopy (TEM), and Fourier transform infrared spectroscopy (FTIR). The phase transformation occurs very rapidly, in less than 1 min, and metastable forsterite relics are not observed after deformation. The grain size of wadsleyite is slightly smaller than the forsterite starting material. The water contents obtained from FTIR analyses in forsterite and wadsleyite are 65-124 wt ppm H2O and 114-736 wt ppm H2O, respectively, which are well below water solubility at similar conditions in the presence of free water. Wadsleyite aggregates display weak CPO patterns with [1 0 0] axes concentrated at low angle to the shear direction, [0 1 0] axes perpendicular to the shear plane and nearly random [0 0 1] axes. Only a few dislocations were observed in wadsleyite with TEM. This observation is consistent with the assumption that most dislocations formed during the initial high-stress stages of these stress-relaxation experiments, were consumed in the phase transformation, probably enhancing the transformation rate. CPO patterns vary as a function of the water content: with increasing water content the density of [1 0 0] axes parallel to the shear direction decreases, and the density of [0 0 1] axes increases. Viscoplastic self-consistent modeling of CPO evolution using previously reported glide systems for wadsleyite, i.e., [1 0 0]{0 k 1} and 1/2 (1 1 1){1 0 1}, cannot reproduce the measured CPO, unless the [0 0 1](0 1 0) system, for which dislocations have not been observed by TEM, is also activated. In addition, wadsleyite grain growth suggests the participation of diffusion-assisted processes in deformation. Calculated anisotropies for P and S-waves using measured CPO are always below 1%. This very low anisotropy is due to both the low finite strain achieved in the experiments, which leads to weak wadsleyite CPO, and to the diluting effect of added majorite. The present experiments emphasize the importance of stress, grain size evolution and water content in the forsterite to wadsleyite phase transformation and subsequent deformation in the transition zone.
ISSN: 0031-9201

hal-00617430
https://hal.archives-ouvertes.fr/hal-00617430
DOI : 10.1016/j.pepi.2010.11.001

Éditeur(s) : HAL CCSD
Résumé : International audience
Increasingly, health risk assessment is addressing multiple pathway exposures to multiple contaminants. We reviewed aggregated exposure and cumulative risk approaches for contemporary and ubiquitous semivolatile organic compounds (SVOC). We identified 22 studies aggregating exposure pathways, and 31 cumulating risk. Exposure aggregation is based on the addition of pathway-specific doses, using kinetic modeling where it exists, and classic external dose equations otherwise. In most cases, exposure is dominated by a single route or source of exposure - mainly the oral pathway - via dietary or non-dietary exposure. Preferential routes and sources of exposure are influenced by SVOC physical-chemical properties such as vapor pressure. The cumulative risk approach for contaminants is based on dose addition. Simple sum of hazard quotient (Hazard Index: HI) is the most commonly used cumulative risk assessment approach, while Relative Potency Factor (RPF) appeared to the best suited - although this calls for a level of toxicological information that limits the number of compounds that can be studied simultaneously. Where both were performed, moving from HI to more refined approach produced similar results. In conclusion, both approaches - exposure aggregation and cumulative risk - rely on simple assumptions. Nevertheless, they allow uncertainty to be reduced, in comparison with source-by-source or chemical-by-chemical approaches.
Environmental Research

hal-01579608
https://hal-univ-rennes1.archives-ouvertes.fr/hal-01579608
DOI : 10.1016/j.envres.2017.06.022
PUBMED : 28732321

Éditeur(s) : HAL CCSD Elsevier
Résumé : International audience
Trivalent inorganic arsenic [As(III)] is an efficient anticancer agent used to treat patients suffering from acute promyelocytic leukemia. Recently, experimental studies have clearly demonstrated that this metalloid can also cure lymphoproliferative and/or pro-inflammatory syndromes in different murine models of chronic immune-mediated diseases. T helper (Th) 1 and Th17 lymphocytes play a central role in development of these diseases, in mice and humans, especially by secreting the potent pro-inflammatory cytokine interferon-γ and IL-17A, respectively. As(III) impairs basic functions of human T cells but its ability to modulate secretion of pro-inflammatory cytokines by differentiated Th lymphocytes is unknown. In the present study, we demonstrate that As(III), used at concentrations clinically achievable in plasma of patients, has no effect on the secretion of interferon-γ from Th1 cells but almost totally blocks the expression and the release of IL-17A from human Th17 lymphocytes co-stimulated for five days with anti-CD3 and anti-CD28 antibodies, in the presence of differentiating cytokines. In addition, As(III) specifically reduces mRNA levels of the retinoic-related orphan receptor (ROR)C gene which encodes RORγt, a key transcription factor controlling optimal IL-17 expression in fully differentiated Th17 cells. The metalloid also blocks initial expression of IL-17 gene induced by the co-stimulation, probably in part by impairing activation of the JNK/c-Jun pathway. In conclusion, our results demonstrate that As(III) represses expression of the major pro-inflammatory cytokine IL-17A produced by human Th17 lymphocytes, thus strengthening the idea that As(III) may be useful to treat inflammatory immune-mediated diseases in humans.
ISSN: 0041-008X

inserm-00872871
http://www.hal.inserm.fr/inserm-00872871
DOI : 10.1016/j.taap.2012.05.004
PUBMED : 22617429

Éditeur(s) : HAL CCSD Elsevier
Résumé : We investigate experimentally the alteration of fractured class-G cement flowed by CO2-rich brine. The experiment mimics a mechanically damaged rough-walled fractured cement annulus at temperature 60 °C and pressure 10 MPa. The experiment consists of flowing a reservoir-equilibrated brine mixed with CO2 (partial pressure of 2.3 MPa) through the fracture of average aperture 14 μm at constant flow rate (100 μL min−1). This flow rate corresponds to pressure gradient representative of an average in situ hydrodynamic condition. Results indicate an intense alteration of the cement with a large removal of mass at the scale of the sample. However, the fracture alteration patterns are triggered by the initial heterogeneity of the fracture aperture; the aperture of the low aperture zones tends to decrease due to calcite precipitation whereas preferential paths develop in the zones of higher aperture associated. Nevertheless, the expected large permeability increase triggered by the mass removal is mitigated by the precipitation of a low density Si-rich amorphous material. The alteration rate will decrease with time because of the increasing distance of diffusion between the fracture where the reactants are actively renewed by advection and the portlandite and C-S-H dissolution fronts. The different zones of reaction can be adequately modeled by a simple 1D diffusion-reaction model using published kinetics coefficients and extrapolation to larger times than the experiment time can be drawn. Altogether, and in addition to the previous studies of the alteration of fractured well cement annulus, this study shows that the leakage potential is strongly controlled by the initial distribution of the aperture along the fracture: low aperture zones will tend to self-heal while localized flow in connected high aperture paths will be perennial.
ISSN: 1750-5836

hal-01346006
https://hal.archives-ouvertes.fr/hal-01346006
DOI : 10.1016/j.ijggc.2016.01.032

Éditeur(s) : HAL CCSD European Geosciences Union
Résumé : International audience
Natural disturbances are the dominant form of forest regeneration and dynamics in unmanaged tropical forests. Monitoring the size distribution of treefall gaps is important to better understand and predict the carbon budget in response to land use and other global changes. In this study, we model the size frequency distribution of natural canopy gaps with a discrete power law distribution. We use a Bayesian framework to introduce and test, using Monte Carlo Markov chain and Kuo–Mallick algorithms, the effect of local physical environment on gap size distribution. We apply our methodological framework to an original light detecting and ranging dataset in which natural forest gaps were delineated over 30 000 ha of unmanaged forest. We highlight strong links between gap size distribution and environment, primarily hydrological conditions and topography, with large gaps being more frequent on floodplains and in wind-exposed areas. In the future, we plan to apply our methodological framework on a larger scale using satellite data. Additionally , although gap size distribution variation is clearly under environmental control, variation in gap size distribution in time should be tested against climate variability.
ISSN: 1726-4170

hal-01590729
https://hal-univ-guyane.archives-ouvertes.fr/hal-01590729
https://hal-univ-guyane.archives-ouvertes.fr/hal-01590729/document
https://hal-univ-guyane.archives-ouvertes.fr/hal-01590729/file/bg-14-353-2017.pdf
DOI : 10.5194/bg-14-353-2017

Éditeur(s) : HAL CCSD Société géologique de France
Résumé : International audience
The present study proposes to estimate the influence of climate, eustatism and local tectonics on the sedimentation of a basin margin at the syn-rift to post-rift transition. For that, paleomagnetic measurements were performed on a marine marly-calcareous sedimentary succession ranging from Upper Oligocene to Lower Miocene and located on the northern margin of the Liguro-Provençal basin. The magnetostratigraphic record is correlated to the reference geomagnetic polarity scale [ATNTS04, Lourens et al. 2004] with the help of biostratigraphy based on calcareous nannofossils and planctonic foraminifers [Oudet et al., 2010]. The resulting age model shows that the 100 m-thick sedimentary succession covers a time span of 5 m.y. from the Late Chattian to the Early Burdigalian. Despite several exposure surfaces and a change in the sedimentation rate, no significant hiatus of sedimentation is documented. In addition, we also estimate the paleoenvironmental evolution through the sedimentary succession. Comparing the dated paleoenvironmental reconstruction with global {delta}18O and sea level curves [Miller et al., 2005], we show that the Carry-le-Rouet succession is an excellent paleoclimatic archive. Indeed, coral reefs developed at the glacial-interglacial stage transition marking the end of the Oligocene. In addition, the most diversified coral reefs occurred during the warmest period of the Aquitanian. During rifting, bathymetric variations recorded in the studied succession are related to local synsedimentary tectonics whereas, during oceanic crust accretion, global sea level changes influence the sedimentation. This result allows to characterise and to accurately date the break-up unconformity at 20.35 Ma.
EISSN: 1777-5817

hal-00619040
https://hal.archives-ouvertes.fr/hal-00619040
DOI : 10.2113/gssgfbull.182.1.37

Éditeur(s) : HAL CCSD Public Library of Science
Résumé : International audience
Cytomegalovirus (CMV) infection is common among HIV-infected patients but its repercussion on the course of CD4+ and CD8+ T cells after cART initiation remains elusive. The French Dat'AIDS cohort enrolled 5,688 patients on first-line cART, from which we selected patients who achieved HIV suppression for at least 12 months without modification of cART, and for whom CMV serostatus was available. Five hundred and three patients fulfilled the selection criteria (74% male, median age 43 yrs, 15.5% CDC stage C), of whom 444 (88.3%) were seropositive for CMV (CMV+). Multivariate analyses using mixed-linear models adjusted for the time from HIV suppression, sex, age, transmission risk group, duration of HIV follow-up, the interaction between time from HIV suppression and CMV+ serol-ogy, and the nadir CD4 count revealed a negative correlation between CMV+ and CD4: CD8 ratio (coeff. =-0.16; p = 0.001). This correlation was also observed among patients displaying optimal CD4 recovery (!500 cells/mm3 at M12; coeff. =-0.24; p = 0.002). Hence, CMV+ serostatus antagonizes normalization of the CD4:CD8 ratio, although further analyses of the impact of co-morbidities that associate with CMV serostatus, like HCV infection, are needed to elucidate this antagonism formally. However, this might reflect a premature T cell senescence, thus advocating for a close monitoring of T cells in CMV co-infected patients. In addition, our results raise the question of the benefit of treatment for asymptomatic CMV co-infection in HIV-infected patients.
ISSN: 1932-6203

inserm-01418700
http://www.hal.inserm.fr/inserm-01418700
http://www.hal.inserm.fr/inserm-01418700/document
http://www.hal.inserm.fr/inserm-01418700/file/journal.pone.0165774.PDF
DOI : 10.1371/journal.pone.0165774

Éditeur(s) : HAL CCSD Springer Verlag
Résumé : International audience
A hallmark of living systems is the management and the storage of information through genetic and epigenetic mechanisms. Although the notion of epigenetics was originally given to any regulation beyond DNA sequence, it has often been restricted to chromatin modifications, supposed to behave as cis-markers, specifying the sets of genes to be expressed or repressed. This definition does not take into account the initial view of epigenetics, based on nonlinear interaction networks whose "attractors" can remain stable without need for any chromatin mark. In addition, most chromatin modifications are the steady state resultants of highly dynamic modification and de-modification activities and, as such, seem poorly appropriate to work as long-term memory keepers. Instead, the basic support of epigenetic memory could remain the attractors, to which chromatin modifications belong as do many other components. The influence of chromatin modifications in memory is highly questionable when envisioned as static structural marks, but can be recovered under the dynamic circuitry perspective, thanks to their self-templating properties. Beside their standard repressive or permissive functions, chromatin modifications can also influence transcription in multiple ways such as: (1) by randomizing or inversely stabilizing gene expression, (2) by mediating cooperativity between pioneer and secondary transcription factors, and (3) in the hysteresis and the ultrasensitivity of gene expression switches, allowing the cells to take unambiguous transcriptional decisions.
ISSN: 1420-682X

hal-01063617
https://hal.archives-ouvertes.fr/hal-01063617
DOI : 10.1007/s00018-012-0923-7
PUBMED : 22331281