Éditeur(s) : HAL CCSD Elsevier
Résumé : International audience
Hepatic drug transporters are now recognized as major actors of hepatobiliary elimination of drugs. Characterization of their regulatory pathways is therefore an important issue. In this context, the present study was designed to analyze the potential regulation of human hepatic transporter expression by protein kinase C (PKC) activation. Treatment by the reference PKC activator phorbol 12-myristate 13-acetate (PMA) for 48 h was shown to decrease mRNA expression of various sinusoidal transporters, including OATP1B1, OATP2B1, NTCP, OCT1 and MRP3, but to increase that of OATP1B3, whereas mRNA expression of canalicular transporters was transiently enhanced (MDR1), decreased (BSEP and MRP2) or unchanged (BCRP) in human hepatoma HepaRG cells. The profile of hepatic transporter mRNA expression changes in PMA-treated HepaRG cells was correlated to that found in PMA-exposed primary human hepatocytes and was similarly observed in response to the PKC-activating marketed drug ingenol mebutate. It was associated with concomitant repression of OATP1B1 and OATP2B1 protein expression and reduction of OATP, OCT1, NTCP and MRP2 activity. The use of chemical PKC inhibitors further suggested a contribution of novel PKCs isoforms to PMA-mediated regulations of transporter mRNA expression. PMA was finally shown to cause epithelial-mesenchymal transition (EMT) in HepaRG cells and exposure to various additional EMT inducers, i.e., hepatocyte growth factor, tumor growth factor-β1 or the HNF4α inhibitor ≡I6015, led to transporter expression alterations highly correlated to those triggered by PMA. Taken together, these data highlight PKC-dependent regulation of human hepatic drug transporter expression, which may be closely linked to EMT triggered by PKC activation
ISSN: 0006-2952

hal-01216205
https://hal-univ-rennes1.archives-ouvertes.fr/hal-01216205
https://hal-univ-rennes1.archives-ouvertes.fr/hal-01216205/document
https://hal-univ-rennes1.archives-ouvertes.fr/hal-01216205/file/Protein%20kinase%20C-dependent%20regulation.pdf
DOI : 10.1016/j.bcp.2015.10.007

Éditeur(s) : HAL CCSD Elsevier
Résumé : International audience
Glioblastoma (GB), the most frequent primary tumor of the central nervous system, remains one of the most lethal human cancers despite intensive researches. Current paradigm in the study of GB has been focused on inter-patient variability and on trying to isolate new classification elements or prognostic factors. Here, using ICPL, a technique for protein relative quantification by mass spectrometry, we investigated protein expression between the four regions of GB on clinically relevant biopsies from 5 patients. We identified 584 non-redundant proteins and 31 proteins were found to be up-regulated in the tumor region compared to the peri-tumoral brain tissue, among which, 24 proteins belong to an interaction network linked to 4 biological processes. The core of this network is mainly constituted of interactions between beta-actin (ACTB) with heat shock proteins (HSP90AA1, HSPA8) and 14-3-3 proteins (YWHAZ, YWHAG, YWHAB). A cluster of three isoforms of the sodium pump α-subunit (ATP1A1, ATP1A2, ATP1A3) was also identified outside this network. The differential expression observed for ACTB and 14-3-3γ was further validated by western blot and/or immunohistochemistry. Our study confirms the identity of previously proposed molecular targets, highlights several functional processes altered in GB such as energy metabolism and synaptic transmission and could thus provide added value to new therapeutic trails.
ISSN: 1874-3919

hal-00877756
https://hal.archives-ouvertes.fr/hal-00877756
DOI : 10.1016/j.jprot.2012.04.034
PUBMED : 22575386

Éditeur(s) : HAL CCSD Ecole nationale du génie rural
Résumé :  
Les sols forestiers de Guyane septentrionale permettent d'illustrer un grand nombre de processus pédogénétiques typiques des milieux tropicaux humides. En profondeur, la roche est transformée en saprolite par la nappe, qui sature toute la porosité. Plus haut, les alternances d'humectation et de dessiccation redistribuent le fer et conduisent à la formation d'un ensemble tacheté aux faciès multiples. Dans les horizons supérieurs, un régime hydrique encore plus contrasté, l'apport de matière organique et l'activité biologique poursuivent l'altération des constituants et remanient considérablement leur organisation ; un mécanisme majeur est l'appauvrissement en fer plus ou moins marqué selon la topographie, allant jusqu'à son élimination totale par podzolisation.
ISSN: 0035-2829

hal-01032210
https://hal.archives-ouvertes.fr/hal-01032210

Éditeur(s) : HAL CCSD Mineralogical Society of America
Résumé : International audience
In situ mineralization of CO2 in ultramafic rock-hosted aquifers is one of the promising solutions for decreasing CO2 concentrations in the atmosphere. Naturally altered ultramafic rocks suggest that carbonation processes are controlled by local heterogeneities in the structure of the rock and fluid transport at the water-rock interfaces. We studied the role of rock crystallographic anisotropy relative to the global fluid flow direction on the mineralization of CO2 by means of electron microscope analyses from the macro- to the micrometer scale (EBSD-FIB). The sample used for the measurements was a hot pressed olivine core percolated by water enriched in CO2 (pCO2 = 10 MPa) at 180 °C. During the percolation experiment, olivine was dissolved and two types of carbonates, dolomite, and magnesite, were precipitated on olivine surfaces. The results showed that the dissolution of olivine is controlled by its crystallographic properties as shown by the development of etch-pits only on the (010)ol planes and with elongated shapes parallel to the [010]ol axes. In contrast, the precipitation of carbonates is governed by hydrodynamic properties. Carbonates are heterogeneously distributed in the percolated rock. They are mainly located along the moderate (for dolomite) and the minor (for magnesite) flow paths, both oriented parallel to the principal fluid flow direction, which allow carbonates to be supplied with divalent cations (e.g., Ca2+, Mg2+, and Fe2+). In these flow paths, carbonate growth is systematically oriented normal to the flow that facilitates the development of chemical gradients with cationic supersaturation conditions for carbonate precipitation near the walls.In natural systems, the (010)ol planes are parallel to the Moho and the (100)ol planes are vertical; our study suggests that flow of CO2-rich fluids will induce precipitation of carbonates localized along, and preferentially clogging, vertical flow paths while favoring olivine dissolution along horizontal fluid pathways. This dual control of structure and fluid flow on carbonation mechanisms could be an important parameter allowing sustainable CO2 storage in peridotites, while limiting the risks of leakage toward the surface.
ISSN: 0003-004X

hal-01143393
https://hal.archives-ouvertes.fr/hal-01143393
DOI : 10.2138/am-2015-4913

Éditeur(s) : HAL CCSD Elsevier Masson
Résumé : National audience
The immune system contributes to maintaining the integrity of the organism. It can be the target of many xenobiotics, and immunotoxicology attempts to analyze the immunological alterations caused by exposure to these xenobiotics. Several environmental pollutants are strongly suspected of acting on the immune system to cause a decrease in the organism protective functions. Thus, polycyclic aromatic hydrocarbons, well known for their carcinogenic effects, have also been shown to induce immunosuppressive effects, pro-inflammatory actions and some hypersensitivity reactions, involved in the development of various pathologies. These different immunotoxic effects have mainly been identified by experimental studies on animals and our knowledge of the effects on humans remains incomplete. To better understand the immunotoxic effects of exposure to environmental pollutants and their consequences for human health, it is appropriate to strengthen immunotoxicology studies on humans.
Le système immunitaire contribue à la surveillance et au maintien de l’intégrité de l’organisme. Il peut être la cible de nombreux xénobiotiques, et l’immunotoxicologie s’attache à étudier les différentes atteintes immunologiques induites par l’exposition à ces xénobiotiques. De nombreux polluants environnementaux sont fortement suspectés d’agir sur le système immunitaire pour entraîner une diminution des fonctions de protection de l’organisme. Ainsi, les hydrocarbures aromatiques polycycliques, bien connus pour leurs effets cancérogènes, se sont révélés également capables d’induire des effets immunosuppresseurs, d’entraîner des actions pro-inflammatoires et de majorer certaines réactions d’hypersensibilité, impliqués dans le développement de diverses pathologies. Ces différents effets immunotoxiques ont surtout été identifiés par des études expérimentales chez l’animal et la transposition de ces effets à l’homme reste incomplète. Pour mieux appréhender les effets immunotoxiques de l’exposition aux polluants environnementaux et leurs conséquences sur la santé humaine, il convient donc de renforcer les études en immunotoxicologie chez l’homme.
ISSN: 1775-8785

hal-01467562
https://hal-univ-rennes1.archives-ouvertes.fr/hal-01467562
https://hal-univ-rennes1.archives-ouvertes.fr/hal-01467562/document
https://hal-univ-rennes1.archives-ouvertes.fr/hal-01467562/file/Effets%20immunotoxiques%20des%20polluants%20environnementaux_accepted.pdf
DOI : 10.1016/j.admp.2016.09.003

Éditeur(s) : HAL CCSD
Résumé : The northern Mirdita ophiolite massifs in Albanian Dinarides formed at a slow-spreading ridge, active during the Jurassic (160–165 Ma). They share a common horizontal Jurassic–Lower Cretaceous sedimentary cover showing that they were not deeply and intrinsically affected by later Alpine thrusting. The western massifs of Mirdita, first oceanic core complex (OCC) and detachment shear zone described in ophiolites, compare with OCCs in slow-spreading ridges and provide continuous exposure of the deep internal structure of this system, revealing its kinematics, thanks to detailed structural mapping in peridotites and gabbros. The Mirdita detachments root in the Moho transition zone (MTZ), a weak zone at the top of asthenospheric mantle, where basaltic melts impregnate dunites. The OCC domes are plagioclase-amphibole–bearing mylonitic peridotites, ∼400 m thick, grading downward within 200 m to harzburgitic mantle. The mylonitic detachments crossed Moho beneath a NNE-SSW–trending ridge. On the western side of OCC domes, the hanging wall of the ridge, crustal gabbros, and basalts are still preserved, despite being deeply affected by hydrothermal alteration. From there, the partially molten MTZ was detached as a shear zone, mixing with lower gabbros. The OCC emerged, migrating upsection and eastward over 5 km. Finally, the OCC front is observed in hornblende-rich syntectonic mylonites derived from upper gabbros and from the overlying former lid. Serpentinization is static within these mylonites. A low-temperature detachment fault is expressed as a sheared antigoritic mélange at the margin of the mylonitic shear zone. Asthenospheric flow in the harzburgitic mantle beneath the ridge of origin has been preserved below the OCC rooting. The dominant asthenospheric flow direction trends parallel to the ridge axis. This mantle flow rotates over 200 m into the low-temperature mylonitic detachments, where OCC motion turns transversal to the ridge. Crystal preferred orientation measurements on six samples point to brown hornblende crystal growth during mylonitic flow and illustrate the change of olivine intra-crystalline slip system in mylonites compared to porphyroclastic harzburgite.
ISSN: 1553-040X

hal-01504440
https://hal.archives-ouvertes.fr/hal-01504440
DOI : 10.1130/GES01383.1

Éditeur(s) : HAL CCSD
Résumé : International audience
Abstract View references (38)This work presents a facile, scalable method for the fabrication of graphene oxide-iron oxide (GO-Fe3O4) nanocomposites produced by co-precipitation of iron ions onto the GO surfaces in basic aqueous media. FTIR and XRD characterisation suggests that Fe3O4 was chemically anchored to the GO sheets, possibly via the carboxyl and hydroxyl groups. Small GO loadings of 0.5 and 1 wt% were not sufficient to alter the Fe3O4 structures. However, increasing the GO loading to 5 and 10 wt% resulted in significant loss in pore volume, thus suggesting the lamellar GO sheets decorated with Fe3O4 were assembling into GO-Fe3O4 nanocomposite stacks. It was found that the GO-Fe3O4 nanocomposites had an enhanced catalytic activity in the heterogeneous Fenton-like oxidation of Acid Orange 7 (AO7) compared to pure GO flat sheets and Fe3O4 nanoparticles. Interestingly, the GO-Fe3O4 nanocomposite with 5 wt% loading ratio exhibited the best catalytic activity with 76% degradation of AO7 dye observed within 90 min of reaction. This was attributed to the synergistic effect of GO by enriching the adsorbed AO7 molecules onto the nanocomposite to be further oxidised in the vicinities of active sites. This finding suggests the promising employment of GO-Fe3O4 nanocomposites in removing organic dyes from polluted water by heterogeneous Fenton-like reaction. © 2014 by American Scientific Publishers.
SCIENCE OF ADVANCED MATERIALS

hal-01683207
https://hal.univ-antilles.fr/hal-01683207
DOI : 10.1166/sam.2014.1812

Éditeur(s) : HAL CCSD Public Library of Science
Résumé : International audience
Accumulating evidence suggests that developmental exposure to environmental chemicals may modify the course of brain development, ultimately leading to neuropsychiatric / neurodegenerative disorders later in life. In the present study, we assessed the impact of one of the most frequently used pesticides in both residential and agricultural applications - the synthetic pyrethroid cypermethrin (CYP) - on developmental neurotoxicity (DNT). Female mice were perinatally exposed to low doses of CYP (5 and 20 mg/kg body weight) from gestation to postnatal day 15. Behavioral analyses were performed during the offspring's early life and during adulthood. Postnatal analyses revealed that perinatal exposure to CYP disturbed motor development without modifying sensory and communicative skills. We found that later in life, CYP-exposed offspring expressed maladaptive behaviors in response to highly challenging tasks and abnormal sociability. Transcriptomic analyses performed in the offspring's brain at the end of the exposure, highlighted mitochondrial dysfunction as a relevant pathomechanism underlying CYP-induced DNT. Interestingly, several genes involved in proteostasis maintenance were also shown to be dysregulated suggesting that alterations in biogenesis, folding, trafficking and degradation of proteins may significantly contribute to CYP-related DNT. From a regulatory perspective, this study highlights that behavioral and transcriptomic analyses are complementary tools providing useful direction for better DNT characterization, and as such, should be used together more systematically.
ISSN: 1932-6203

hal-01616489
https://hal-univ-rennes1.archives-ouvertes.fr/hal-01616489
DOI : 10.1371/journal.pone.0184475
PUBMED : 29020013

Éditeur(s) : HAL CCSD Elsevier
Résumé : International audience
Several epidemiologic studies have shown an interactive effect of heavy smoking and heavy alcohol drinking on the development of hepatocellular carcinoma. It has also been recently described that chronic hepatocyte death can trigger excessive compensatory proliferation resulting later in the formation of tumors in mouse liver. As we previously demonstrated that both benzo[a]pyrene (B[a]P), an environmental agent found in cigarette smoke, and ethanol possess similar targets, especially oxidative stress, to trigger death of liver cells, we decided to study here the cellular and molecular mechanisms of the effects of B[a]P/ethanol coexposure on cell death. After an 18-h incubation with 100nM B[a]P, primary rat hepatocytes were supplemented with 50mM ethanol for 5 or 8h. B[a]P/ethanol coexposure led to a greater apoptotic cell death that could be linked to an increase in lipid peroxidation. Plasma membrane remodeling, as depicted by membrane fluidity elevation and physicochemical alterations in lipid rafts, appeared to play a key role, because both toxicants acted with specific complementary effects. Membrane remodeling was shown to induce an accumulation of lysosomes leading to an important increase in low-molecular-weight iron cellular content. Finally, ethanol metabolism, but not that of B[a]P, by providing reactive oxygen species, induced the ultimate toxic process. Indeed, in lysosomes, ethanol promoted the Fenton reaction, lipid peroxidation, and membrane permeabilization, thereby triggering cell death. To conclude, B[a]P exposure, by depleting hepatocyte membrane cholesterol content, would constitute a favorable ground for a later toxic insult such as ethanol intoxication. Membrane stabilization of both plasma membrane and lysosomes might be a potential target for further investigation considering cytoprotective strategies.
ISSN: 0891-5849

hal-01018147
https://hal-univ-rennes1.archives-ouvertes.fr/hal-01018147
https://hal-univ-rennes1.archives-ouvertes.fr/hal-01018147/document
https://hal-univ-rennes1.archives-ouvertes.fr/hal-01018147/file/Cooperative-interaction_final.pdf
PUBMED : 24681337

Éditeur(s) : HAL CCSD Frontiers
Résumé : International audience
Estrogens can induce rapid, short-lived physiological and behavioral responses, in addition to their slow, but long-term, effects at the transcriptional level. To be functionally relevant, these effects should be associated with rapid modulations of estrogens concentrations. 17β-estradiol is synthesized by the enzyme aromatase, using testosterone as a substrate, but can also be degraded into catechol-estrogens via hydroxylation by the same enzyme, leading to an increase or decrease in estrogens concentration, respectively. The first evidence that aromatase activity (AA) can be rapidly modulated came from experiments performed in Japanese quail hypothalamus homogenates. This rapid modulation is triggered by calcium-dependent phosphorylations and was confirmed in other tissues and species. The mechanisms controlling the phosphorylation status, the targeted amino acid residues and the reversibility seem to vary depending of the tissues and is discussed in this review. We currently do not know whether the phosphorylation of the same amino acid affects both aromatase and/or hydroxylase activities or whether these residues are different. These processes provide a new general mechanism by which local estrogen concentration can be rapidly altered in the brain and other tissues.
ISSN: 1662-4548

Droits : http://creativecommons.org/licenses/by/
hal-01142062
https://hal-univ-rennes1.archives-ouvertes.fr/hal-01142062
https://hal-univ-rennes1.archives-ouvertes.fr/hal-01142062/document
https://hal-univ-rennes1.archives-ouvertes.fr/hal-01142062/file/fnins-09-00083.pdf
PUBMED : 25852459

Éditeur(s) : HAL CCSD Elsevier
Résumé : International audience
We analyzed twelve fault gouge samples from the Bogd fault in south-western Mongolia to understand the origin and behavior of clay minerals. The investigation relies on x-ray powder diffraction (XRD), scanning electron microscopy (SEM), transmission electron microscopy (TEM) and high energy synchrotron x-ray diffraction methods to investigate microstructure and preferred orientation. Smectite (montmorillonite), illite-smectite mixed layers, illite-mica and kaolinite are the major clay components, in addition to quartz and feldspars, which are present in all samples. The observations suggest that the protoliths and the fault rocks were highly altered by fluids. The fluid-rock interactions allow clay minerals to form, as well as alter feldspars to precipitate kaolinite and montmorillonite. Thus, newly formed clay minerals are heterogeneously distributed in the fault zone. The decrease of montmorillonite component of some of the highly deformed samples also suggests that dehydration processes during deformation were leading to illite precipitation. Based on synchrotron x-ray diffraction data, the degree of preferred orientation of constituent clay minerals is weak, with maxima for (001) ranging from 1.3 to 2.6 multiples of a random distribution (m.r.d). Co-existing quartz and feldspars have random orientation distributions. Microstructure and texture observations of the gouges from the foliated microscopic zone, alternating with micrometric isotropic clay-rich area, also indicate that the Bogd fault experienced brittle and ductile deformation episodes. The clay minerals may contribute to a slip weakening behavior of the fault.
ISSN: 0191-8141

insu-00667194
https://hal-insu.archives-ouvertes.fr/insu-00667194
DOI : 10.1016/j.jsg.2011.10.006

Éditeur(s) : HAL CCSD Elsevier
Résumé : International audience
Mitochondrial DNA integrity is ensured by several nuclear-encoded proteins in vertebrates, and a number of mtDNA alterations in human diseases, including deletions and duplications, have been suspected to result from errors in the mitochondrial recombination pathway. However, the presence of the latter system is still a matter of controversy as RecA proteins display various functions in vitro. In Escherichia coli, RecA plays a central role in homologous recombination by pairing and transferring a single strand to a homologous duplex DNA. To address indirectly the issue of a mitochondrial recombination pathway in vivo, we have constructed a chimeric gene containing an N terminal mitochondrial targeting sequence and the E. coli RecA gene. Cells were transfected by the recombinant plasmid, then tested for their mtDNA repair upon bleomycin treatment. We found an increased repair rate of the mitochondrial DNA in cells expressing RecA as compared to control cells. These results indicate that the transfected cells display an improved mtDNA repair replication pathway due to the exogenous RecA, likely in synergy with an endogenous rate-limiting mitochondrial recombination pathway.
ISSN: 0027-5107

hal-01680533
https://hal.univ-antilles.fr/hal-01680533
PUBMED : 11356332

Éditeur(s) : HAL CCSD Elsevier
Résumé : International audience
Carbonation of peridotite is a widespread process in nature, with emerging societal and environmental implications through geological storage of CO2. We studied the micro- and nano-scale structures associated with the carbonation of peridotite pervaded by a CO2-rich water and the induced changes of its hydrodynamic properties. Focused ion beam (FIB) nanotomography was coupled with scanning electron microscopy (SEM) and transmission electron microscopy (TEM) observations. An experimental sample of dunite core percolated by a CO2-rich fluid (pCO2 = 11 MPa) at 160 °C was compared with a naturally carbonated sample from the Atlantic Ridge (Exp. IODP 304). Results of the 8-days percolation experiment show continuous alteration of olivine into magnesite locally associated with a poorly crystalline serpentine-type mineral referred to as proto-serpentine. The experiment is also characterized by an overall decrease in rock porosity (from 7.7 to 6.4%) and permeability (from 8.5 × 10− 16 to 1.7 × 10− 16 m2). The primary inter-granular porosity (1 to 10 μm) is progressively filled with magnesite, leading to the overall decrease of permeability. At the same time, a secondary small-scale porosity (10 nm to 1 μm) is created at the tip of the dissolution features along the (010) cleavage planes of olivine grains. Carbonate crystals grow within these etch-pit structures behind the dissolution front. Dissolution and precipitation occur at a relative rate that maintains the secondary porosity over the timescale of the experiment (i.e. carbonate growth has to be the slowest one). This process is interpreted as an interface coupled dissolution-precipitation mechanism of olivine carbonation. Proto-serpentine is only formed in highly reduced flow areas, such as small olivine etch-pits (10 to 500 nm wide), which are more isolated from CO2 input. SEM-FIB analyses show the 3D preferential orientation of the dissolution-precipitation planes within a given olivine grain. It suggests the formation of lateral connections between the etch-pits (infra-micrometric “channels”) providing access to reactants and removing reaction products at the reaction sites. The experimental results show that olivine dissolution and magnesite precipitation converge to a quasi-steady state resulting in a constant carbonation rate controlled by reaction kinetics and not by mass transfer for this flow regime. The system self-regulates, independently of the porosity change at least during this incipient stage of olivine carbonation. Similar textures are observed in natural samples collected at 170 m-depth in the vicinity of veinlets driving late fluids down into the cooling oceanic lithosphere. This suggests that similar mechanisms are active in nature and can play an important role in the late, low temperature alteration stages of fractured peridotite. These results also highlight the critical role of nm-scale spatial arrangement of dissolution and precipitation features on mass transfers and on the sustainability of olivine carbonation through coupled dissolution-precipitation processes.
ISSN: 0009-2541

hal-01684733
https://hal.archives-ouvertes.fr/hal-01684733
DOI : 10.1016/j.chemgeo.2017.11.013

Éditeur(s) : HAL CCSD Elsevier Elsevier Masson
Résumé : International audience
La maladie alcoolique du foie se caractérise par une stéatose précoce, qui, pour seulement 20 % des buveurs excessifs, peut évoluer vers la stéatohépatite ou la cirrhose. À l'heure actuelle, la description des facteurs de risque et des mécanismes cellulaires et moléculaires impliqués dans la progression de la maladie restent toujours incomplète. Ces dernières années, les radeaux lipidiques, microstructures de la membrane plasmique, ont été décrits comme une nouvelle cible de l'exposition à l'éthanol. Or les acides gras polyinsaturés oméga 3, présents naturellement dans certains aliments comme les poissons gras, se sont révélés être capables de modifier le microenvironnement physicochimique de ces microstructures avec des effets bénéfiques pour certaines pathologies comme l'inflammation ou le cancer. L'objectif de cette revue est ainsi d'établir un lien entre les altérations membranaires induites par les oméga 3 et leurs effets sur les mécanismes moléculaires impliqués dans la progression de la maladie alcoolique du foie.
ISSN: 0985-0562

hal-00966797
https://hal.archives-ouvertes.fr/hal-00966797
https://hal.archives-ouvertes.fr/hal-00966797/document
https://hal.archives-ouvertes.fr/hal-00966797/file/Acides_gras_polyinsaturA_s_omA_ga_3_et_toxicitA_hA_patique_de_la_A_thanol_rA_le_du_remodelage_membranaire.pdf
DOI : 10.1016/j.nupar.2013.10.003

Éditeur(s) : Université des Sciences et Techniques du Languedoc, Montpellier
Résumé : On étudie les parasitoses à Marteilia refringens et à Haplosporidium armoricanum de l’huître plate européenne Ostrea edulis. L’étude de M. refringens précise certains aspects de la biologie de l’agent infectieux. Elle montre que les modalités du cycle de développement du parasite et l‘ultrastructure des stades les plus évolués de la sporogenèse peuvent être modifiées selon les conditions hydrobiologiques du milieu dans lequel est cultivé l’hôte. Elle établi que l’action du parasite conduit à la destruction des épithéliums digestifs de l’huître et s’exerce en interférant avec le métabolisme protéique d’O. edulis. Elle précise l‘ultrastructure des cellules impliquées dans des proliférations hémocytaires atypiques associées à la parasitose et établit la nature néoplasique du phénomène. L’étiologie de l’hémocytose n’est toutefois pas déterminée. Enfin elle met en évidence un cas d‘hyperparasitisme de M. refringens par une Microsporidie. L’étude ultrastructurale de la sporogenèse et des effets pathogènes du Protozoaire vis à vis de l’hôte nous conduit à identifier l’hyperparasite comme Nosema ormieresi et montre que certains stade de M. refringens peuvent être fortement altérés par l’infestation microsporidienne. L’étude d’une Haplosporidie observée chez O. edulis du bassin d’Arcachon (France) nous amène à comparer la sporagenèse du parasite avec celle d’autres Haplosporidies d’invertébrés marins. Les caractéristiques de la spore nous incitent à inclure le parasite dans le genre Haplosporidium et le rapprochement du Protozoaire avec Minchinia armoricana conduit à l’identifier comme Haplosporidium armoricanum. L’étude de la pathogenèse des infections à M. refringens et à H. armoricanum permet de préciser les relations entre les parasites étudiés et leur hôte commun l’huître plate et les interactions hôte-parasite-milieu pouvant intervenir dans le caractère des deux maladies.

Droits : The Author, Univ. Montpellier, ISTPM
http://archimer.ifremer.fr/doc/00083/19411/17022.pdf
http://archimer.ifremer.fr/doc/00083/19411/

Éditeur(s) : CM. 1982/K : 8
Résumé : Since 1978, the habits of the french fishermen working with bottom trawl in the Irish Sea and in the Celtic Sea were altered (CHARUAU et M0RIZUR, 198l) under the pressure of the new mesh regulation. Two facts are pointed out: - the shifting, stabilized during 1980 and 198l, of a part of the effort of the vessels fishing for gadoids, from the area VIIa to the areaVIIf - a tendancy inside the area VIIg of a part of the effort of the Nephrops trawlers to be transferred on benthic fishes when the yield of Nephrops is low.
Cette communication décrit l'évolution des habitudes des pêcheurs français travaillant au chalut de fond en Mer d'Irlande (division CIEM VIIfa) et sur le Plateau Celtique (divisions CIEM VIIf et g) sous la pression des nouvelles réglementations de irai liage. Elle fait suite à la communication de CHARUAU et M0RIZUR(198l) sur le même sujet. Deux phénomènes simultanés se sont produits depuis 1978 : - un repli qui semble s'être stabilisé entre 1980 et 198l, d'une partie de la flottille des bateaux spécialisés dans la pêche du poisson, de la division VIIa vers la division VIIf, - un report, à l'intérieur de la division VIIg, d'une partie de l'effort des langoustiniers vers le poisson. Ce changement qui s'explique surtout par des raisons socio-économiques n'est pas analysé. Seules les tendances par rapport a 1980 sont constatées.

Droits : info:eu-repo/semantics/openAccess
http://archimer.ifremer.fr/doc/1982/acte-2342.pdf
http://archimer.ifremer.fr/doc/00000/2342/

Éditeur(s) : HAL CCSD
Résumé : International audience
The Achmmach tin mineralization (NE of theMoroccan Central Massif) is associated with tourmaline-richalteration halos, veins, and faults hosted in sandstones andmetapelites of the Upper Visean-Namurian. These depositsare reported to be late Hercynian in age and related to theemplacement of late-orogenic granite not outcropping in thestudied area. Structural and paragenetic studies of theAchmmach tin deposit were conducted in order to establisha general model of the mineralization. From field constraints,the late Hercynian phase is marked by a transition fromtranspression to extension with deformation conditions evolvingfrom ductile to brittle environments. The transpression(horizontal shortening direction roughly trending E-W) is coevalwith the emplacement of the first tourmaline halos alongseveral conjugated trends (N070, N020, and N120).Thereafter, a tourmaline-rich breccia formed in response tothe fracturing of early tourmaline-altered rocks.Subsequently, during the extensional phase, these structureswere reactivated as normal faults and breccias, allowing theformation of the main tin mineralization (cassiterite) associatedwith a wide variety of sulfides (arsenopyrite, chalcopyrite,sphalerite, galena, pyrrhotite, bismuthinite, pyrite, and stannite). This evolution ends with fluorite and carbonate deposition.The hydrothermal fluid flow velocity, calculated byapplying statistical measures on the tourmaline growth bands,varies with the lithology. Values are lower in metapelites andhigher in breccia. In the general evolution model proposedhere, tourmaline alteration makes the rock more competent,allowing for brittle fracturing and generation of open spacewhere the main Sn mineralization was precipitated.
Mineralium Deposita

insu-01240481
https://hal-insu.archives-ouvertes.fr/insu-01240481
DOI : 10.1007/s00126-015-0613-0

Éditeur(s) : HAL CCSD Elsevier
Résumé : International audience
Aryl hydrocarbon receptor-dependent genomic effects of environmental polycyclic aromatic hydrocarbons (PAHs) have been shown to be modulated by non-genomic protein kinase C (PKC)-related pathways. The present study was designed to determine whether PKC activation may also impair estrogenic genomic response triggered by PAHs. Treatment by the PKC activator phorbol 12-myristate 13-acetate (PMA) was found to markedly and differentially impair the up-regulation of estrogenic markers triggered by the estrogenic PAH benzanthracene (BZA) in cultured human mammary cells; BZA-mediated mRNA up-regulation of pS2 and amphiregulin was thus increased, whereas that of progesterone receptor and CXCL12 was repressed. BZA/PMA cotreatment however failed to alter BZA-mediated increase of activity of a luciferase gene reporter construct driven by an estrogen response element, thus discarding any global effect of PMA toward BZA-triggered estrogen receptor activation. Various chemicals inhibiting PKCs or extracellular signal-regulated kinase (ERK) as well as the knock-down of PKCδ expression counteracted the PMA-mediated increase of pS2 mRNA up-regulation triggered by BZA, demonstrating that it was dependent on PKCs, including PKCδ isoform, and ERKs. This non-genomic modulation of estrogenic effects of PAHs by PKC activation may have to be considered when considering the deleterious effects of these environmental contaminants towards the endocrine system.
ISSN: 0887-2333

inserm-00872862
http://www.hal.inserm.fr/inserm-00872862
DOI : 10.1016/j.tiv.2012.05.006
PUBMED : 22643241

Éditeur(s) : HAL CCSD Elsevier
Résumé : Rh (Rhesus) is a major blood group system in man, which is clinically significant in transfusion medicine. Rh antigens are carried by an oligomer of two major erythroid specific polypeptides, the Rh (D and CcEe) proteins and the RhAG glycoprotein, that shared a common predicted structure with 12 transmembrane a-helices (M0 to M11). Non erythroid homologues of these proteins have been identified (RhBG and RhCG), notably in diverse organs specialized in ammonia production and excretion, such as kidney, liver and intestine. Phylogenetic studies and experimental evidence have shown that these proteins belong to the Amt/Mep/Rh protein superfamily of ammonium/methylammonium permease, but another view suggests that Rh proteins might function as CO2 gas channels. Until recently no information on the structure of these proteins were available. However, in the last two years, new insight has been gained into the structural features of Rh proteins (through the determination of the crystal structures of bacterial AmtB and archeaebacterial Amt-1. Here, models of the subunit and oligomeric architecture of human Rh proteins are proposed, based on a refined alignment with and crystal structure of the bacterial ammonia transporter AmtB, a member of the Amt/ Mep/Rh superfamily. This alignment was performed considering invariant structural features, which were revealed through Hydrophobic Cluster Analysis, and led to propose alternative predictions for the less conserved regions, particularly in the N-terminal sequences. The Rh models, on which an additional Rh-specific, N-terminal helix M0 was tentatively positioned, were further assessed through the consideration of biochemical and immunochemical data, as well as of stereochemical and topological constraints. These models highlighted some Rh specific features that have not yet been reported. Among these, are the prediction of some critical residues, which may play a role in the channel function, but also in the stability of the subunit structure and oligomeric assembly. These results provide a basis to further understand the structure/function relationships of Rh proteins, and the alterations occurring in variant phenotypes.
ISSN: 1246-7820

hal-00068842
https://hal.archives-ouvertes.fr/hal-00068842

Éditeur(s) : HAL CCSD Springer Verlag
Résumé : International audience
Chromatin modification enzymes are important regulators of gene expression and some are evolutionarily conserved from yeast to human. Saccharomyces cerevisiae is a major model organism for genome-wide studies that aim at the identification of target genes under the control of conserved epigenetic regulators. Ume6 interacts with the upstream repressor site 1 (URS1) and represses transcription by recruiting both the conserved histone deacetylase Rpd3 (through the co-repressor Sin3) and the chromatin-remodeling factor Isw2. Cells lacking Ume6 are defective in growth, stress response, and meiotic development. RNA profiling studies and in vivo protein-DNA binding assays identified mRNAs or transcript isoforms that are directly repressed by Ume6 in mitosis. However, a comprehensive understanding of the transcriptional alterations, which underlie the complex ume6Δ mutant phenotype during fermentation, respiration, or sporulation, is lacking. We report the protein-coding transcriptome of a diploid MAT a/α wild-type and ume6/ume6 mutant strains cultured in rich media with glucose or acetate as a carbon source, or sporulation-inducing medium. We distinguished direct from indirect effects on mRNA levels by combining GeneChip data with URS1 motif predictions and published high-throughput in vivo Ume6-DNA binding data. To gain insight into the molecular interactions between successive waves of Ume6-dependent meiotic genes, we integrated expression data with information on protein networks. Our work identifies novel Ume6 repressed genes during growth and development and reveals a strong effect of the carbon source on the derepression pattern of transcripts in growing and developmentally arrested ume6/ume6 mutant cells. Since yeast is a useful model organism for chromatin-mediated effects on gene expression, our results provide a rich source for further genetic and molecular biological work on the regulation of cell growth and cell differentiation in eukaryotes.
ISSN: 1617-4615

hal-01151619
https://hal-univ-rennes1.archives-ouvertes.fr/hal-01151619
DOI : 10.1007/s00438-015-1051-5
PUBMED : 25957495