Éditeur(s) : HAL CCSD Mineralogical Society of America
Résumé : International audience
Hydrogen distribution between nominally anhydrous minerals (NAMs) of a garnet-lherzolite under subsolidus conditions has been investigated. Separated NAMs from a garnet-peridotite from Patagonia (Chile) are annealed together (olivine, orthopyroxene, clinopyroxene, and garnet) using a piston-cylinder at 3 GPa and 1100 °C using talc-pyrex cell assembly for 10, 25, and 100 h. The talc-pyrex assembly provides enough hydrogen in the system to re-equilibrate the hydrogen concentrations at high pressure. The three coexisting nominally anhydrous minerals (NAMs, i.e., olivine, orthopyroxene, and clinopyroxene) were successfully analyzed using FTIR. The resulting hydrogen concentrations exceed significantly the initial hydrogen concentration by a factor of 13 for olivine and a factor of 3 for both pyroxenes. Once mineral-specific infrared calibrations are applied, the average concentrations in NAMs are 115 ± 12 ppm wt H2O for olivine, 635 ± 75 ppm wt H2O for orthopyroxene, and 1214 ± 137 ppm wt H2O for clinopyroxene, garnet grains are dry. Since local equilibrium seems achieved over time (for 100 h), the calculated concentration ratios are interpreted as mineral-to-mineral hydrogen partition coefficients (i.e., Nernst’s law) for a garnet-peridotite assemblage. It yields, based on mineral-specific infrared calibrations, DOpx/Ol = 5 ± 1, DCpx/Ol = 10 ± 2, and DCpx/Opx = 1.9 ± 0.4. While DCpx/Opx is in agreement (within error) with previous results from experimental studies and concentration ratios observed in mantle-derived peridotites, the DPx/Ol from this study are significantly lower than the values reported from mantle-derived xenoliths and also at odd with several previous experimental studies where melt and/or hydrous minerals co-exists with NAMs. The results confirm the sensitivity of hydrogen incorporation in olivine regarding the amount of water-derived species (H) in the system and/or the amount of water in the coexisting silicate melt. The results are in agreement with an important but incomplete dehydration of mantle-derived olivine occurring at depth, during transport by the host magma or during slow lava flow cooling at the surface. The rapid concentration modification in mantle pyroxenes also points out that pyroxenes might not be a hydrogen recorder as reliable as previously thought.
ISSN: 0003-004X

hal-01622132
https://hal.archives-ouvertes.fr/hal-01622132
DOI : 10.2138/am-2017-6089

Éditeur(s) : HAL CCSD Wiley
Résumé : International audience
Pathogenic agents can be very hard to detect, and usually they do not cause illness for several hours or days. To improve the speed and the accuracy of detection tests and satisfy the needs of early diagnosis, molecular biology methods such as PCR are now used. However, selecting a proper target gene and designing good primers is often not easy. We present a dedicated website, http://patho-genes.org, where we provide every sequence, functional annotation, published primer and relevant article for every annotated gene of major pathogenic bacterial species listed as key agents to be used for a bioterrorism attack. Each published primer was analysed to determine its melting temperature, its specificity and its coverage (i.e. its sensitivity against every allele of its target gene). Data generated have been organized in the form of data sheet for each gene, which are available through multiple browser panels and query systems.
ISSN: 1751-7915

hal-01546181
https://hal.archives-ouvertes.fr/hal-01546181
DOI : 10.1111/j.1751-7915.2012.00353.x

Éditeur(s) : HAL CCSD
Résumé : International audience
The challenge is to implement research that can estimate the consequences ofclimate changes in terms of impact on terrestrial environments and resources.Emphasis should be placed on regions dependent on natural resources and forwhich demographic pressure is strong. Simulations obtained from climate modelprojections (using different Representative Concentration Pathways (RCPs))predict that the Mediterranean basin and its southern periphery are particularlyvulnerable to water resources and environmental impact (IPCC, AR5, 2013).An annual rainfall decrease by 30% is found for the projection period 2070-2099(IPCC, AR5, 2013) associated with a decrease in water resources by 30 to 50%(Milano, 2012). In addition, several studies using regional atmospheric modelsindicate an increase in the precipitation inter-annual variability with extremeevents and a spatial heterogeneous signature, superimposed on a decrease in thetotal precipitation amount (Giorgi and Lionello, 2008; Raible et al. 2010).Currently, regional climate projections are highly sensitive to the climate modelused. In particular, spatial resolution as well as local climate conditions seemto impact significantly on the simulations (Jacob et al. 2014).The Mediterranean region, at the interface between arid and temperate climateswith several mountainous areas, is a complex climate system affected by theinteractions between mid-latitude and sub-tropical processes. In this context,Morocco, located at the transition between a temperate climate to the North anda tropical climate to the south constitutes a key area for an impact and sensitivitystudy to global climate changes. The climate is influenced by the Atlantic Ocean,the Mediterranean Sea and the Sahara, together with a very steep orography inthe Atlas region. The precipitation distribution is therefore characterised by greatspatial variability, and exhibits a marked seasonality, a strong inter-annualvariability (Ouda et al. 2005) and in general a pronounced gradient from northto south and west to east. At a broader scale, Morocco is located on the subtropicalsubsidence path and between the Acores High and the Saharan Low (Agoussine,2003). Several studies have also identified strong links with inter-annualprecipitation variability and NAO index (Knippertz, 2003) as well as remoteclimate modes (Esper et al. 2007).Continental climate variability at a local/regional scale, if it is to be integratedin climate predictions, needs to be supported by long-term observation.Meteorological stations in Morocco provide climatic data mainly for the last40 years with only a few stations located in the mountainous region (Tramblayet al. 2012; 2013; Driouech et al. 2010). This climate database is also supportedby the IAEA network providing stations for which isotope tracers have beenapplied to daily/monthly rain and water vapour samples over 2 to 3 years between2000 and 2004. Besides the poor coverage of instrumented areas, lacustrinesystems can provide a climatic data set that offers access to short and long-termtime series of climate parameters when knowledge of modern lake water balanceis combined with lacustrine sedimentary-climate records. Lake sediment recordsideally provide high resolution climate/environmental information of the last10,000 years (Magny et al. 2013). This time interval (corresponding to theHolocene) is a key period to investigate short and long-term climate variabilityand to improve prediction in a warming climate.In this study we present an integrated approach focusing on a mountainous lake(Aguelmam Azigza). The modern lake system study is based on site monitoring(2012-2016) and available regional hydro-climatic data. These data show thatlake level changes during the instrumented period were mainly driven byprecipitation following the high inter-annual variability. These data are thencompared with accurately dated short sediment cores retrieved in the same lake.Micro-scale geochemical and sedimentological analyses of these sequencesenable us to identify various sedimentary facies that can be linked with periodsof high (low) lake levels over the past decades.
The Mediterranean Region under Climate ChangeLa méditerranée face au changement climatique

insu-01394690
https://hal-insu.archives-ouvertes.fr/insu-01394690
https://hal-insu.archives-ouvertes.fr/insu-01394690/document
https://hal-insu.archives-ouvertes.fr/insu-01394690/file/Vidaletal.2016-COP22-Chap1.pdf

Éditeur(s) : HAL CCSD Public Library of Science
Résumé : International audience
Millimeter Waves (MMW) will be used in the next-generation of high-speed wireless technologies, especially in future Ultra-Broadband small cells in 5G cellular networks. Therefore, their biocompatibilities must be evaluated prior to their massive deployment. Using a microarray- based approach, we analyzed modifications to the whole genome of a human keratinocyte model that was exposed at 60.4 GHz-MMW at an incident power density (IPD) of 20 mW/cm2 for 3 hours in athermic conditions. No keratinocyte transcriptome modifications were observed. We tested the effects of MMWs on cell metabolism by co-treating MMW-exposed cells with a glycolysis inhibitor, 2-deoxyglucose (2dG, 20 mM for 3 hours), and whole genome expression was evaluated along with the ATP content.We found that the 2dG treatment decreased the cellular ATP content and induced a high modification in the transcriptome (632 coding genes). The affected genes were associated with transcriptional repression, cellular communication and endoplasmic reticulum homeostasis. The MMW/ 2dG co-treatment did not alter the keratinocyte ATP content, but it did slightly alter the transcriptome, which reflected the capacity of MMWto interfere with the bioenergetic stress response. The RT-PCR-based validation confirmed 6 MMW-sensitive genes (SOCS3, SPRY2, TRIB1, FAM46A, CSRNP1 and PPP1R15A) during the 2dG treatment. These 6 genes encoded transcription factors or inhibitors of cytokine pathways, which raised questions regarding the potential impact of long-term or chronic MMW exposure on metabolically stressed cells. © 2016 Soubere Mahamoud et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
ISSN: 1932-6203

hal-01367149
https://hal-univ-rennes1.archives-ouvertes.fr/hal-01367149
DOI : 10.1371/journal.pone.0160810
PUBMED : 27529420

Éditeur(s) : HAL CCSD Springer/Terra Scientific Publishing Company
Résumé : We analyse the results of Thellier palaeointensity experiments modified so that the measured magnetisation at any stage may be decomposed into components of natural remanent magnetisation (NRM) and laboratory thermal remanent magnetisation (TRM). We demonstrate that the very long high temperature tails of pTRM acquired by multidomain (MD) grains may be detected in Thellier experiments without recourse to explicit pTRM tail check measurements. This can save time in the experimental process and provides a more sensitive indication of how MD behaviour may affect that particular experiment than either pTRM tail checks or the observation of zigzagging in an IZZI experiment. We observe that the action of imparting a pTRM tail also involves some removal of NRM. However, the pTRM tails are not the analogues of classical overprints as may be intuitively expected but instead appear to favour remagnetisation over demagnetisation. This and other observed behaviour is not consistent with any phenomenological model of MD TRM but is consistent with predictions made by kinematic theory.
ISSN: 1343-8832

hal-00407946
https://hal.archives-ouvertes.fr/hal-00407946

Éditeur(s) : HAL CCSD Springer Verlag
Résumé : International audience
: Human exposure to xenobiotics is usually estimated by indirect methods. Biological monitoring has emerged during the last decade to improve assessment of exposure. However, biomonitoring is still an analytical challenge, because the amounts of sample available are often very small yet analysis must be as thorough and sensitive as possible. The purpose of this work was to develop an untargeted "exposomics" approach by using ultra-high-performance liquid chromatography coupled to high-resolution mass spectrometry (UHPLC-HRMS), which was applied to the characterization of pesticide metabolites in urine from pregnant women from a French epidemiological cohort. An upgradable list of pesticides commonly used on different crops, with their metabolites (more than 400 substances) was produced. Raw MS data were then processed to extract signals from these substances. Metabolites were identified by tandem mass spectrometry; putative identifications were validated by comparison with standards and metabolites generated by experiments on animals. Finally, signals of identified compounds were statistically analyzed by use of multivariate methods. This enabled discrimination of exposure groups, defined by indirect methods, on the basis of four metabolites from two fungicides (azoxystrobin, fenpropimorph) used in cereal production. This original approach applied to pesticide exposure can be extended to a variety of contaminant families for upstream evaluation of exposure from food and the environment.
ISSN: 1618-2642

hal-00875889
https://hal.archives-ouvertes.fr/hal-00875889
DOI : 10.1007/s00216-013-7136-2
PUBMED : 23892877

Éditeur(s) : HAL CCSD Oxford University Press (OUP)
Résumé : International audience
Hepatocellular carcinoma treatment by arterial infusion of cis-diamminedichloroplatinum-II (cisplatin) exhibits certain therapeutic efficacy. However, optimizations are required and the mechanisms underlying cisplatin proapoptotic effect remain unclear. The mitogen-activated protein kinase (MAPK) pathway plays a key role in cell response to cisplatin and the functional specificity of the isoform MAPK/ERK kinase 1 and 2 (MEK1/2) and ERK1/2 could influence this response. The individual contribution of each kinase on cisplatin-induced death was thus analyzed after a transient or stable specific inhibition by RNA interference in the human hepatocellular carcinoma cells Huh-7 or in knockout mice. We demonstrated here that ERK1 played a predominant role over ERK2 in cisplatin-induced death, whereas MEK1 and MEK2 acted in a redundant manner. Indeed, at clinically relevant concentrations of cisplatin, ERK1 silencing alone was sufficient to protect cells from cisplatin-induced death both in vitro, in Huh-7 cells and ERK1(-/-) hepatocytes, and in vivo, in ERK1-deficient mice. Moreover, we showed that ERK1 activity correlated with the induction level of the proapoptotic BH3-only protein Noxa, a critical mediator of cisplatin toxicity. On the contrary, ERK2 inhibition upregulated ERK1 activity, favored Noxa induction and sensitized hepatocarcinoma cells to cisplatin. Our results point to a crucial role of ERK1 in cisplatin-induced proapoptotic signal and lead us to propose that ERK2-specific targeting could improve the efficacy of cisplatin therapy by increasing ERK1 prodeath functions.
ISSN: 0143-3334

inserm-00814096
http://www.hal.inserm.fr/inserm-00814096
DOI : 10.1093/carcin/bgs317
PUBMED : 23042098

Éditeur(s) : Springer
Résumé : Series of sensitivity tests were performed with a z-coordinate, global eddy-permitting (1/4 degrees) ocean/sea-ice model (the ORCA-R025 model configuration developed for the DRAKKAR project) to carefully evaluate the impact of recent state-of-the-art numerical schemes on model solutions. The combination of an energy-enstrophy conserving (EEN) scheme for momentum advection with a partial step (PS) representation of the bottom topography yields significant improvements in the mean circulation. Well known biases in the representation of western boundary currents, such as in the Atlantic the detachment of the Gulf Stream, the path of the North Atlantic Current, the location of the Confluence, and the strength of the Zapiola Eddy in the south Atlantic, are partly corrected. Similar improvements are found in the Pacific, Indian, and Southern Oceans, and characteristics of the mean flow are generally much closer to observations. Comparisons with other state-of-the-art models show that the ORCA-R025 configuration generally performs better at similar resolution. In addition, the model solution is often comparable to solutions obtained at 1/6 or 1/10 degrees resolution in some aspects concerning mean flow patterns and distribution of eddy kinetic energy. Although the reasons for these improvements are not analyzed in detail in this paper, evidence is shown that the combination of EEN with PS reduces numerical noise near the bottom, which is likely to affect current-topography interactions in a systematic way. We conclude that significant corrections of the mean biases presently seen in general circulation model solutions at eddy-permitting resolution can still be expected from the development of numerical methods, which represent an alternative to increasing resolution.
Ocean Dynamics (1616-7341) (Springer), 2006-12 , Vol. 56 , N. 5-6 , P. 543-567

Droits : © Springer 2006. Part of Springer Science+Business Media
http://archimer.ifremer.fr/doc/2006/publication-3514.pdf
DOI:10.1007/s10236-006-0082-1
http://archimer.ifremer.fr/doc/00000/3514/

Éditeur(s) : HAL CCSD Endocrine Society
Résumé : International audience
: Context:Masculinization depends on the fetal testis. Exposure of the human fetus during pregnancy to paracetamol or/and to other mild-analgesics is associated with an increased risk of cryptorchidism.Objective:We aimed at determining whether mild analgesics disrupted the morphology and endocrine function of the human testis.Design:We used an in vitro system based on the culture of human fetal testes exposed or not to paracetamol, its metabolite AM404, aspirin, indomethacin, ketoconazole at 10(-4) M to 10(-7) M.Setting:The study was conducted at the University of Rennes I.Patients/Participants:Human fetal testes were from pregnant women following induced abortion, between 7 and 12 weeks of gestation (GW).Main Outcome Measures:Testosterone (radioimmunoassay: RIA), Anti-Müllerian Hormone (AMH; ELISA), insulin-like factor 3 (INSL3; RIA), prostaglandin D2 and E2 (PGD2 and PGE2, respectively; ELISA), were assayed in the media. Testicular cells were counted using histology and image analysis. The possible nuclear receptor-mediated activities of the analgesics were investigated using reporter cell lines expressing estrogen (ERs), androgen (AR) and peroxysome proliferator-activated γ ( PPARγ) receptors.Results:Indomethacin and aspirin stimulated testosterone production, particularly by the younger testes (8-9 GW vs 10-12 GW). Paracetamol, AM404 and ketoconazole decreased INSL3 levels. Aspirin stimulated, whereas ketoconazole inhibited AMH production. PGE2 levels were inhibited by paracetamol, and aspirin in the 7-12 GW testes, and by indomethacin but only in 7-9.86 GW old testes. The inhibitory trends seen for PGD2 were not statistically significant.Conclusions:Analgesics at concentrations relevant to human exposure cause endocrine disturbances in the fetal testis. We suggest that the fetal human testis displays slight critical age windows for sensitivity to direct exposure to aspirin, indomethacin and paracetamol. The analgesic-induced inhibition of INSL3 may be the mechanism by which analgesics increase the risk of cryptorchidism. Greater caution is required concerning consumption of analgesics during pregnancy.
ISSN: 0021-972X

inserm-00876125
http://www.hal.inserm.fr/inserm-00876125
DOI : 10.1210/jc.2013-2531
PUBMED : 24030937

Éditeur(s) : HAL CCSD Springer-Verlag
Résumé : International audience
High-resolution borehole strainmeters are usually installed in tectonically active regions in order to detect slow-slip events, and to estimate slow transients related to earthquake swarms. However, they are also sensitive to other numerous influences, internal or external. Furthermore, the quality of their coupling to the rock through cementation, and the mechanical properties of the rock mass around them, have a critical influence on their records. Many of the existing strainmeters present such problems, and the correction for these effects often remains a challenge. In this paper, we present the analysis of the records of a high-resolution borehole dilatometer (Sacks–Evertson), located in the seismically active rift of Corinth (Greece) (station TRZ in the Trizonia island). We show that the instrument suffers from an only partial-solid coupling, and that the nearby sea tides have a direct (through elastic response) and indirect (through pore-pressure diffusion) effect on the dilatation signal, which adds up to the solid tidal strain source. We propose a methodology that allows, in a first step, to better separate the internal (solid tide) from the external (air pressure, sea level) influences, by calculating a frequency-dependent transfer function outside the range of the tidal periods. We then extrapolate this function, in particular at the tidal periods. In a second step, the resulting variation with frequency of the coupling coefficients with sea level led us to estimate the proportion of instrument not solidly cemented to rock (thus in contact with water pore pressure), which is about 90 % of the total height. Despite the small proportion of solid coupling, the sensor resolution remains very good up to a few tens of hours of a time period, thanks to the confining effects of the rocks on the local pore pressure. These results allow us to correct for the external effects, and reduce the associated variance by 80–90 % (in the period range of minutes to days). The empirical correction of the sea level effect could be explained using a simple Boussinesq’s approximation and 1D pore-pressure diffusion model, which contributed to better constraint of some of the poro-elastic parameters in the vicinity of the instrument. After correction, the solid tidal signal at the 24-h period is almost anti-correlated with those of the theoretical solid tide. This surprising result is consistent with a similar anti-correlation observed for the longest period surface waves (200 s) comparing the TRZ dilatometer signals to the strain measured by a nearby borehole strainmeter (MOK, 15 km). This could be related to the presence of a shallow fault close to the instrument, which would creep in response to seismic wave-related stress.
ISSN: 0033-4533

hal-01122853
https://hal.archives-ouvertes.fr/hal-01122853
DOI : 10.1007/s00024-013-0742-2

Éditeur(s) : Elsevier
Résumé : Choice of sampling method and survey period can have an important impact on the perception of the structure and dynamics of an ecological community. For the Celtic Sea fish assemblage we compared data obtained by three different trawl surveys: an autumn groundfish survey with a GOV trawl, and a spring and an autumn groundfish survey, both carried out with a Portuguese high-headline trawl. Time-series of abundance estimates were not consistent among surveys for all species and were generally very noisy. An analysis of variance components showed that the sampling method contributed more to the variance in abundance estimates compared to survey period, interannual variability, or even sampling variance. Overall community assessments based on indicators such as proportions of non-commercial and piscivorous species, and the proportion of benthic species showed similar trends for all data series. The shape of the size spectrum based on abundances per length class summed over all fish species, although stable over time, was highly sensitive to the sampling method. With the exception of size spectra, community indicators for marine fish assemblages monitored by surveys seem to be robust to survey period and trawling gear, but species abundance trends are method dependent.
ICES Journal of Marine Science (1054-3139) (Elsevier), 2004-05 , Vol. 61 , N. 3 , P. 351-362

Droits : 2004 International Council for the Exploration of the Sea. Published by Elsevier Science Ltd
http://archimer.ifremer.fr/doc/2004/publication-899.pdf
DOI:10.1016/j.icesjms.2004.01.004
http://archimer.ifremer.fr/doc/00000/899/

Résumé : Pour beaucoup de cultures, un sol non travaillé est une alternative de plus en plus crédible au travail intensif du sol, en particulier pour des raisons environnementales. Mais l’ananas (ananas comosus) présente un enracinement fragile très sensible à la structure, ce qui motive souvent un travail important du sol avant plantation. L’alternative d’une plantation sans travail du sol ne va donc pas de soi. L’objet de cette thèse est de répondre à la question de la faisabilité d’une culture d’ananas sans travail du sol, en remplaçant ce dernier par une plante de service « décompactante » dont les traits racinaires seraient favorables à la (re)structuration d’un sol compact. Le travail de cette thèse a donc porté sur l’évaluation de plusieurs espèces candidates, puis sur l’étude, au champ, des effets sur le sol de la plus prometteuse (Stylosanthes guianensis ). Enfin, dans le cadre d’un essai au champ comparant un système de culture innovant ananas sans travail du sol, on a étudié l’effet de la plante de service sur le fonctionnement de la culture de l’ananas. Dans un premier essai, c’est le Stylosanthes guianensis qui, comparé à huit autres espèces (Arachis pintoï, Brachiaria decumbens, Cajanus cajan, Crotalaria juncea, Cynodon dactylon, Eleusine coracana, Pueraria phaseoloides, et le maïs), a montré les traits racinaires les plus favorables à la structuration d’un sol compact. Les valeurs supérieures du diamètre racinaire moyen et de la densité de longueur racinaire (DRL) caractérisent les principaux traits impliqués. Dans un deuxième essai, les mesures de conductivité hydraulique, d’indice des vides du sol et d’analyse d’images de blocs de sol imprégné sur la répartition surfacique des différents types de porosité ont montré que la culture du Stylosanthes guianensis avait augmenté l’indice des vides du sol et provoqué l’apparition d’une porosité fissurale de grande taille, contribuant ainsi à l’amélioration de la structure du sol. Enfin, un troisième essai mettant en comparaison (i) un système de culture innovant où la culture d’ananas est implantée sans travail du sol après une culture de S. guianensis restructurante et (ii) un système conventionnel comportant un travail profond du sol (et pas de plante de service) a montré que le rendement en fruit est similaire dans les deux systèmes. Cet essai a permis de vérifier que l’enracinement de l’ananas en condition de sol non travaillé bénéficiait du précédent S. guianensis.
For many crops, direct drilling is a well-tried alternative facing the damaging effects of intensive tillage, mainly for environmental causes. But, pineapple (ananas comosus), presents a fragile rooting system which is very sensitive to soil structure. This leads frequently to intensive soil tillage before planting. Direct drilling is not so evident. The aim of this thesis is to give an answer to the feasibility of a no till system for pineapple cultivation, by using a plant with favorable roots traits for compacted soil (re)structuration. This work consisted in evaluating several candidate species, followed by the study, on the field, of the effects the most promising on soil (Stylosanthes guianensis). Finally, through a field experiment, comparing an innovating no till pineapple cultivation system we studied the use effects of Stylosanthes guianensis on the pineapple crop functioning. In a first experiment, Stylosanthes guianensis compared with eight other species (Arachis pintoï, Brachiaria decumbens, Cajanus cajan, Crotalaria juncea, Cynodon dactylon, Eleusine coracana, Pueraria phaseoloides and corn) showed better roots traits for structuring a compacted soil. Measures of average root diameter and root length density are the main implicated roots traits. In a second experiment, the measures of hydraulic conductivity, of the soil void ratio and the analysis of blocks of resin-impregnated soil on the surface distribution of the different type of porosity, all of this showed that Stylosanthes guianensis had increased the soil void ratio and had caused the creation of large-sized cracked porosity, thus contributing to the improvement of the soil structure. Finally, a third experiment involving a comparison between (i) an innovating cultivation system where pineapple is growing in a no till soil after a structuring crop of S. guianensis and (ii) a conventional system with deep tillage (without structuring crop), showed similar fruit yield. This experiment showed evidence that the rooting of pineapple in no till soil benefited from the previous Stylosanthes.

http://www.theses.fr/2014AGUY0725/document

Éditeur(s) : HAL CCSD Oxford University Press (OUP)
Résumé : International audience
Adverse pregnancy outcomes entail a large health burden for the mother and offspring; a part of it might be avoided by better understanding the role of environmental factors in their etiology. Our aims were to review the assessment tools to characterize fecundity troubles and pregnancy-related outcomes in human populations and their sensitivity to environmental factors. For each outcome, we reviewed the possible study designs, main sources of bias, and their suggested cures. In terms of study design, for most pregnancy outcomes, cohorts with recruitment early during or even before pregnancy allow efficient characterization of pregnancy-related events, time-varying confounders, and in utero exposures that may impact birth outcomes and child health. Studies on congenital anomalies require specific designs, assessment of anomalies in medical pregnancy terminations, and, for congenital anomalies diagnosed postnatally, follow-up during several months after birth. Statistical analyses should take into account environmental exposures during the relevant time windows; survival models are an appropriate approach for fecundity, fetal loss, and gestational duration/preterm delivery. Analysis of gestational duration could distinguish pregnancies according to delivery induction (and possibly pregnancy-related conditions). In conclusion, careful design and analysis are required to better characterize environmental effects on human reproduction.
ISSN: 0193-936X

hal-01002724
https://hal-univ-rennes1.archives-ouvertes.fr/hal-01002724
DOI : 10.1093/epirev/mxt011
PUBMED : 24363355

Éditeur(s) : HAL CCSD Elsevier
Résumé : International audience
Fault affecting silicoclastic sediments are commonly enriched in clay minerals. Clays are sensitive to fluid–rock interactions and deformation mechanisms; in this paper, they are used as proxy for fault activity and behavior. The present study focuses on clay mineral assemblages from the Point Vert normal fault zone located in the Annot sandstones, a Priabonian-Rupelian turbidite succession of the Alpine foredeep in SE France. In this area, the Annot sandstones were buried around 6–8 km below the front of Alpine nappes soon after their deposition and exhumed during the middle-late Miocene. The fault affects arkosic sandstone beds alternating with pelitic layers, and displays throw of about thirty meters. The fault core zone comprises intensely foliated sandstones bounding a corridor of gouge about 20 cm thick. The foliated sandstones display clay concentration along S–C structures characterized by dissolution of K-feldspar and their replacement by mica, associated with quartz pressure solution, intense microfracturation and quartz vein precipitation. The gouge is formed by a clayey matrix containing fragments of foliated sandstones and pelites. However, a detailed petrographical investigation suggests complex polyphase deformation processes. Optical and SEM observations show that the clay minerals fraction of all studied rocks (pelites and sandstones from the damage and core zones of the fault) is dominated by white micas and chlorite. These minerals have two different origins: detrital and newly-formed. Detrital micas are identified by their larger shape and their chemical composition with a lower Fe–Mg content than the newly-formed white micas. In the foliated sandstones, newly-formed white micas are concentrated along S–C structures or replace K-feldspar. Both types of newly formed micas display the same chemical composition confirmed microstructural observations suggesting that they formed in the same conditions. They have the following structural formulas: Na0.05 K0.86 (Al 1.77 Fe0.08 Mg0.15) (Si3.22 Al0.78) O10 (OH)2. They are enriched in Fe and Mg compared to the detrital micas. Newly-formed chlorites are associated with micas along the shear planes. According to microprobe analyses, they present the following structural formula: (Al1,48 Fe2,50 Mg1,84) (Si2,82 Al1,18) O10 (OH)8. All these data suggest that these clay minerals are synkinematic and registered the fault activity. In the gouge samples, illite and chlorite are the major clay minerals; smectite is locally present in some samples.In the foliated sandstones, Kubler Index (KI) ((001) XRD peak width at half height) data and thermodynamic calculations from synkinematic chlorite chemistry suggest that the main fault deformation occurred under temperatures around 220 °C (diagenesis to anchizone boundary). KI measured on pelites and sandstones from the hanging and footwall, display similar values coherent with the maximal burial temperature of the Annot sandstones in this area. The gouge samples have a higher KI index, which could be explained by a reactivation of the fault at lower temperatures during the exhumation of the Annot sandstones formation
ISSN: 0191-8141

hal-01172423
https://hal.archives-ouvertes.fr/hal-01172423
DOI : 10.1016/j.jsg.2015.03.012

Éditeur(s) : HAL CCSD American Society for Microbiology
Résumé : International audience
Pneumocystis jirovecii pneumonia (PCP) is a life-threatening infection in immunocompromised patients. Quantitative real-time PCR (qPCR) is more sensitive than microscopic examination for the detection of P. jirovecii but also detects colonized patients. Hence, its positive predictive value (PPV) needs evaluation. In this 4-year prospective observational study, all immunocompromised patients with acute respiratory symptoms who were investigated for PCP were included, totaling 659 patients (814 bronchoalveolar lavage fluid samples). Patients with negative microscopy but positive qPCR were classified through medical chart review as having retained PCP, possible PCP, or colonization, and their clinical outcomes were compared to those of patients with microscopically proven PCP. Overall, 119 patients were included for analysis, of whom 35, 41, and 43 were classified as having retained PCP, possible PCP, and colonization, respectively. The 35 patients with retained PCP had clinical findings similar to those with microscopically proven PCP but lower fungal loads (P extless 0.001) and were mainly non-HIV-infected patients (P extless 0.05). Although the mean amplification threshold was higher in colonized patients, it was not possible to determine a discriminant qPCR cutoff. The PPV of qPCR in patients with negative microscopy were 29.4% and 63.8% when considering retained PCP and retained plus possible PCP, respectively. Patients with possible PCP had a higher mortality rate than patients with retained PCP or colonization (63% versus 3% and 16%, respectively); patients who died had not received co-trimoxazole. In conclusion, qPCR is a useful tool to diagnose PCP in non-HIV patients, and treatment might be better targeted through a multicomponent algorithm including both clinical/radiological parameters and qPCR results.
ISSN: 0095-1137

hal-01063906
https://hal.archives-ouvertes.fr/hal-01063906
DOI : 10.1128/JCM.01480-14
PUBMED : 25009050

Éditeur(s) : HAL CCSD World Scientific
Résumé : The present paper presents a laboratory experiment of rip current circulations over a moveable bed. The rip current characteristics over four distinct beach morphologies, exhibiting more or less developed nature-like bar-rip morphology,were investigated. For each video run, the same off shore shore-normal waves were generated by the wave maker with the same mean water level in order to study the sensitivity of the rip current characteristics as a function of the beach morphology only. In each case, a 1-hour video run was used to track a large number (~30) of drifters released within the surf zone. Image coordinates were then rectifie d to still water level Cartesian coordinates to compute drifter velocities, mean characteristics and surf zone rete ntion rates. Results show the presence of classic rip current patterns with counter-rotating cells and a relatively narrowoffshore-directed jet with, for three of the situations, a reasonably symmetric shape. Non-surprisingly, it was found that rip current intensity increases with increasing relative depth of the rip channel. The wave-driven circulations were strongly unstable. Computed standard deviation in fl ow intensity and direction provides high resolution information on the spatial variability of the rip current insta bilities with, for instance, highly-pulsating and weakly directionally variable offshore-directed flow in the rip channel. Conversely to what was previously hypothesized in the literature, there was hardly trace of vortices being shed offs hore and drifters exiting the surf zone compartment were not systematically caught by a pulsating jet. The cause for drifter exiting the semi-enclosed surf zone compartment remains, however, elusive and deserves further investigations. The computed surf zone retention rates (~90%) were of the order of those previously observed in the field, with no clear relationship with the mean rip current velocity or relative depth of the rip channel. Further video-runs will have to be analyzed to explore potential explanations
Proceedings of Coastal Dynamics 2009 Impacts of Human Activities on Dynamic Coastal Processes

ISBN : 978-981-4282-46
hal-00854534
https://hal.archives-ouvertes.fr/hal-00854534

Éditeur(s) : HAL CCSD Elsevier Masson
Résumé : National audience
OBJECTIVES: Medical tools to determine the imminence of unplanned out-of-hospital birth have not been validated. The main goal of this study was to analyze assessments of the imminence of these deliveries, by methods such as the Manilas score and the need to push. PATIENTS AND METHODS: All calls made to the Emergency Medical Aid Service in Guadeloupe concerning risks of unplanned delivery between January 1st and June 30th 2011 were reviewed retrospectively, and 69 of the 85 cases cared for by the hospital mobile emergency unit were included. RESULTS: Risks of unplanned out-of-hospital deliveries represented 1.9% of the calls during the study period. The Malinas score was used for dispatching in only 13% and was not used for intervention in any case. It was estimated that the need-to-push feeling was used for 58% (dispatching) and 54.5% (intervention) of patients. Its sensitivity, specificity and predictive values were higher than those of the Malinas score in dispatching and substantially higher in intervention. CONCLUSION: These results argue for the development of tools for estimating the imminence of unplanned delivery. Such tools should be applicable in extra-hospital emergency situations to ensure their use in practice.
ISSN: 0368-2315

hal-01063667
https://hal.archives-ouvertes.fr/hal-01063667
DOI : 10.1016/j.jgyn.2013.01.009
PUBMED : 23490410

Éditeur(s) : HAL CCSD
Résumé : International audience
Extreme rainfall events can trigger numerous landslides in mountainous areas and a prolonged increase of river sediment load. The resulting mass transfer at the Earth surface in turn induces stress changes at depth, which could be sufficient to trigger shallow earthquakes. The 2009 Morakot typhoon represents a good case study as it delivered 3 m of precipitation in 3 days and caused some of the most intense erosion ever recorded. Analysis of seismicity time-series before and after the Morakot typhoon reveals a systematic increase of shallow (i.e. 0-15 km of depth) earthquake frequency in the vicinity of the areas displaying a high spatial density of landslides. This step-like increase in frequency lasts for at least 2-3 years and does not follow an Omori-type aftershock sequence. Rather, it is associated to a step change of the Gutenberg-Richter b-value of the earthquake catalog. Both changes occurred in mountainous areas of southwest Taiwan, where typhoon Morakot caused extensive landsliding. These spatial and temporal correlations strongly suggest a causal relationship between the Morakot-triggered landslides and the increase of earthquake frequency and their associated b-value. We propose that the progressive removal of landslide materials from the steep mountain landscape by river sediment transport acts as an approximately constant increase of the stress rate with respect to pre-typhoon conditions, and that this in turn causes a step-wise increase in earthquake frequency. To test this hypothesis, we investigate the response of a rate-and-state fault to stress changes using a 2-D continuum elasto-dynamic model. Consistent with the results of Ader et al. (2013), our preliminary results show a step-like increase of earthquake frequency in response to a step-like decrease of the fault normal stress. We also investigate the sensitivity of the amplitude and time-scale of the earthquake frequency increase to the amplitude of the normal stress change and to rheological parameters. Our study offers new insights on the potential influence of extreme erosional events on the short-time scale dynamics of faults and earthquakes.
American Geophysical Union Fall Meeting 2016
San Francisco, United States

insu-01416987
https://hal-insu.archives-ouvertes.fr/insu-01416987

Éditeur(s) : HAL CCSD National Institute of Environmental Health Sciences
Résumé : International audience
BACKGROUND: Glycol ethers are present in a wide range of occupational and domestic products. Animal studies have suggested that some of them may affect ovarian function. OBJECTIVE: To study the relation between women's exposure to glycol ethers and time to pregnancy. METHODS: Urine from randomly selected women in the PELAGIE mother-child cohort who had samples collected before 19 weeks of gestation was tested to measure eight glycol ether metabolites by chromatography coupled to mass spectrometry. Time to pregnancy was collected at the beginning of the pregnancy by asking women how many months they took to conceive. Associations between metabolite levels and time to pregnancy were estimated in 519 women with complete data using discrete-time Cox proportional hazards models to adjust for potential confounders. RESULTS: Glycol ether metabolites were detected in 6% (for ethoxyacetic acid) to 93% (for phenoxyacetic and butoxyacetic acids) of urine samples. Phenoxyacetic acid was the only metabolite with a statistically significant association with longer time to pregnancy (fecundability OR 0.82; 95% CI: 0.63, 1.06 for the second and third quartile combined; fecundability OR 0.70; 95% CI: 0.52, 0.95 for a fourth-quartile (≥1.38 mg/L) vs. first-quartile concentration (<0.14 mg/L)). This association remained stable after multiple sensitivity analyses. CONCLUSION: Phenoxyacetic acid, which was present in most of the urine samples tested in our study, was associated with increased time to pregnancy. This metabolite and its main parent compound, 2-phenoxyethanol, are plausible causes of decreased fecundability, but may also be surrogates for potential co-exposures frequently present in cosmetics.
ISSN: 0091-6765

hal-00866023
https://hal-univ-rennes1.archives-ouvertes.fr/hal-00866023
https://hal-univ-rennes1.archives-ouvertes.fr/hal-00866023/document
https://hal-univ-rennes1.archives-ouvertes.fr/hal-00866023/file/Urinary_Glycol_Ether-accepted.pdf
DOI : 10.1289/ehp.1206103

Éditeur(s) : HAL CCSD Elsevier
Résumé : International audience
To evaluate the performance of downhole and surface geophysical monitoring methods, a series of shallow gas injection-monitoring experiments has been performed in a coastal saline aquifer at Maguelone, France. The recorded data include pressure measurements with a Westbay multilevel completion and CO2 saturation at an observation well derived from electrical resistivity with a modified Waxman-Smits (MWS) model. In this work, the aim is to develop a simulation model capturing the gas transport behavior and consistent with field data. For this purpose, the simulation of the CO2 injection experiment is carried out with two conceptual models, a homogeneous model and a heterogeneous model treated with multiple realization Monte Carlo simulations. Numerical simulator TOUGH2 with the equation of state module EOS7C is used for the simulations. Comparison of the model results with field data suggests that the pressure responses are captured with relatively good accuracy. Similarly, the model also provides an overall reasonable agreement and correct order of magnitude for predicted gas saturation values. However, as the heterogeneity pattern in the field data remains largely unknown, the model predictions can only be used to capture the mean behavior as well as to provide insights into how heterogeneity can influence the system behavior, by means of sensitivity analyses of the influence of heterogeneities on individual realizations.
ISSN: 1750-5836

hal-01467240
https://hal.archives-ouvertes.fr/hal-01467240
DOI : 10.1016/j.ijggc.2016.09.006