Éditeur(s) : HAL CCSD Wiley
Résumé : International audience
Among the environmental threats to coral reef health, temperature and ultraviolet increases have been proposed as major agents, although the relative contribution of each in the cnidarian/zooxanthellae symbiosis breakdown has been poorly addressed. We have investigated the transcriptomic response to thermal stress, with and without ultraviolet radiation (UVR), in the symbiotic sea anemone Anemonia viridis. Using the Oligo2K A.similar to viridis microarray, dedicated to genes potentially involved in the symbiosis interaction, we monitored the gene expression profiles after 1, 2 and 5 days of stresses that further lead to massive losses of zooxanthellae. Each stress showed a specific gene expression profile with very little overlap. We showed that the major response to thermal stress is immediate (24 h) but returns to the baseline gene expression profile after 2 days. UVR alone has little effect but potentiates thermal stress, as a second response at 5 days was observed when the two stresses were coupled. Several pathways were highlighted, such as mesoglea loosening, cell death and calcium homeostasis and described in more details. Finally, we showed that the dermatopontin gene family, potentially involved in collagen fibrillogenesis, issued from actinarian-specific duplication events, with one member preferentially expressed in the gastroderm and specifically responding to stress.
ISSN: 0962-1083

hal-01546174
https://hal.archives-ouvertes.fr/hal-01546174
DOI : 10.1111/j.1365-294X.2012.05458.x

Éditeur(s) : HAL CCSD Elsevier
Résumé : International audience
B-type olivine fabrics are pervasive within highly depleted dunites of the small-sized Imono peridotite body located within the subduction-type Sanbagawa metamorphic belt of the southwest Japan arc. The dunites contain various microstructures, ranging from porphyroclastic to fine-grained intensely sheared textures. The Mg/(Mg+Fe) atomic ratios (Fo number) of olivine within these dunites are consistently around 0.9, as are the Cr/(Cr+Al) atomic ratios (Cr number) of chromian spinel, suggesting their evolution from a highly depleted magma (boninite). These data provide strong thermal constraints on the formation of the highly depleted dunites,as their formation requires hot, hydrous, shallow mantle (> 1250 degrees C at < 30 km depth) in the mantle wedge. Because the Sanbagawa metamorphic belt finally entrained these peridotites during progressive retrogression, B-type olivine fabrics probably developed in the fore-arc side of the subduction zone, above or along the subducting slab, possibly in association with dehydration fluids derived from the slab. The previously documented small magnitude of S-wave splitting can be explained by the seismic properties of B-type peridotites within an anisotropic layer of approximately several kilometers in thickness, oriented by flow parallel to the subducting slab, under maximum temperatures of 880-1030 degrees C depending on the flow stress. These findings indicate that such a B-type layer could constitute a dominant source of seismic S- and P-wave anisotropy in mantle wedge regions. (c) 2008 Elsevier B.V. All rights reserved.
ISSN: 0012-821X

hal-00412594
https://hal.archives-ouvertes.fr/hal-00412594
DOI : 10.1016/j.epsl.2008.06.014

Éditeur(s) : HAL CCSD Springer
Résumé : International audience
Thermoelastic behavior of different marble types was analyzed using computational modeling and experimental measurements. Eight marble samples with different composition, grain size, grain boundary geometry, and texture were investigated. Calcitic and dolomitic marbles were considered. The average grain size varies from 75 μm to 1.75 mm; grain boundary geometry differs from nearly equigranular straight grain boundaries to inequigranular-interlobate grain boundaries. Four typical marble texture types were observed by EBSD measurements: weak texture; strong texture; girdle texture and high-temperature texture. These crystallographic orientations were used in conjunction with microstructure-based finite element analysis to compute the thermoelastic responses of marble upon heating. Microstructural response maps highlight regions and conditions in the marble fabric that are susceptible to degradation phenomena. This behavior was compared to the measured thermal expansion behavior, which shows increasing residual strains upon repetitive heating-cooling cycles. The thermal expansion behavior as a function of temperature changes can be classified into four categories: (a) isotropic thermal expansion with small or no residual strain; (b) anisotropic thermal expansion with small or no residual strain; (c) isotropic thermal expansion with a residual strain; and (d) anisotropic thermal expansion with residual strain. Thermal expansion coefficients were calculated for both simulated and experimental data and also modeled from the texture using the MTEX software. Fabric parameters control the amount and directional dependence of the thermal expansion. Marbles with strong texture show higher directional dependence of the thermal expansion coefficients and have smaller microstructural values of the maximum principal stress and strain energy density, the main precursors of microcracking throughout the marble fabric. In contrast, marbles with weak texture show isotropic thermal expansion behavior, have a higher propensity to microcracking, and exhibit higher values of maximum principal stress and strain energy density. Good agreement between the experimental and computational results is observed, demonstrating that microstructure-based finite-element simulations are an excellent tool for elucidating influences of rock fabric on thermoelastic behavior.
ISSN: 1866-6280

hal-00858095
https://hal.archives-ouvertes.fr/hal-00858095
DOI : 10.1007/s12665-013-2406-z

Éditeur(s) : HAL CCSD Elsevier
Résumé : International audience
The Tropical climate imposes a high level of physiological stress, which could modify the target heart rate in training load prescription, as the recommendations are often determined by maximal oxygen uptake testing in temperature-neutral laboratories. To test this hypothesis, 7 highlevel cyclists performed two randomised maximal tests in neutral (19.2±0.9 ◦C; 51.7±1.3% RH) and Tropical environment (25.8±1.1 ◦C; 63.7±2.3% RH). Neither maximal oxygen uptake nor ventilatory threshold was influenced by the environmental conditions. However, ventilation (p < 0.005) and the respiratory equivalent in O2 (p < 0.05) were significantly higher in the Tropical environment, whereas maximal power output and the time to attain maximal oxygen uptake were significantly lower (p < 0.05 for both). Moreover, the ventilatory cost of cycling (expressed in LW−1) was significantly greater in the Tropical condition (0.40±0.03 LW−1 vs. 0.32±0.05 LW−1, in Tropical vs. Neutral; condition effect: p < 0.005; condition×time: p < 0.001). Rectal temperature was influenced by neither the environmental conditions nor exercise (36.7±0.1 and 37.0±0.1 ◦Cvs. 36.8±0.1 and 37.1±0.2 ◦C, in Tropical vs. Neutral, before and after exercise) but was influenced by condition×time (p < 0.05). The heart rate (HR) values usually used for training prescription were not significantly different (154±5 bpm vs. 156±4 bpm and 172±4 bpm vs. 167±4 bpm in Tropical vs. Neutral climate, for the first and second thresholds, respectively). We concluded that the usual parameters measured during maximal exercise to establish training programs are not impaired in moderate Tropical environment. Nevertheless, the thermal stress attested by the increased ventilatory cost of cycling could have prevented the cyclists from performing a true maximal test in Tropical conditions.
ISSN: 1440-2440

hal-00703461
https://hal.univ-antilles.fr/hal-00703461
DOI : 10.1016/j.jsams.2009.11.004

Éditeur(s) : HAL CCSD
Résumé : The ecological success of cnidarian-dinoflagellate symbiosis mainly relies on nutrient recycling. Environmental changes, such as global warming or pollution, often result to symbiosis breakdown, also called cnidarian bleaching. The understanding of mechanisms regulating the symbiosis establishment, maintenance and breakdown is essential to prevent massive bleaching phenomena. In this respect, my PhD project focused on the characterization of chemical mediators expressed in the sea anemone Anemonia viridis, their localization and their modulation by stress conditions. A comparative study was first conducted to characterize the chemical mediators and analyze their distribution within the symbiotic sea anemone. We described a great abundance and diversity of lipids in A. viridis tissues. From these results, we proposed possible transfers of FAs between the symbiotic partners. A thermal stress and a chemical stress have also been applied in laboratory-controlled conditions in order to induce symbiosis breakdown and bleaching of the sea anemones, in order to correlate A. viridis metabolome to its symbiotic status. A mapping of these metabolites has been performed by MALDI-MSI of tentacle cross-sections, as well as their evolution following stress. Some betaine lipids have thus been proposed as short-term indicators of stress. A. viridis stress response has also been evaluated with a lipidomic approach, and allowed to identify 3 lipid indicators of early stress response based on membrane fluidity markers. Overall, this study provides insight on key chemical mediators that may regulate the symbiosis maintenance, and may contribute to the symbiosis breakdown.
Le succès évolutif des Cnidaires symbiotiques réside en grande partie dans leurs échanges trophiques établis avec les Dinoflagellés du genre Symbiodinium. Cependant, le réchauffement climatique global ainsi que les pollutions ont un impact fort sur les écosystèmes coralliens, notamment en conduisant à la rupture de la symbiose, phénomène appelé blanchissement. La compréhension des mécanismes qui régissent l’établissement, le maintien et la rupture de la symbiose est essentielle à la prévention des épisodes de blanchissement massif. Dans ce contexte, les objectifs de mon projet de thèse sont de caractériser les médiateurs chimiques de l’anémone de mer Anemonia viridis, de les localiser, et d’analyser leur réponse face à un stress. Parmi les composés caractérisés, les lipides et les bétaïnes sont les plus abondants et présentent une grande diversité. Certains sont transférés des symbiotes vers l’hôte. Des anémones de mer ont ensuite été traitées en laboratoire afin de provoquer la rupture de la symbiose et le blanchissement des individus. Une étude cinétique a été menée par une approche globale comparative identique à celle réalisée sur l’anémone symbiotique. Par ailleurs, une cartographie de l’évolution de composés clé a été réalisée par MALDI-MSI. La réponse au stress a été évaluée et a permis d’identifier des lipides de bétaïne et trois indicateurs lipidiques comme marqueurs de réponse précoce au stress. L’ensemble de ces résultats apporte de nouveaux éléments de réponse concernant le rôle des médiateurs chimiques clés dans le maintien de la symbiose, ainsi que leur influence sur sa rupture.
https://tel.archives-ouvertes.fr/tel-01310668

NNT : 2015NICE4108
tel-01310668
https://tel.archives-ouvertes.fr/tel-01310668
https://tel.archives-ouvertes.fr/tel-01310668/document
https://tel.archives-ouvertes.fr/tel-01310668/file/2015NICE4108.pdf

Éditeur(s) : HAL CCSD Elsevier
Résumé : International audience
Cnidarians living in symbiosis with photosynthetic dinoflagellates (commonly named zooxanthellae) are exposed to high concentrations of reactive oxygen species (ROS) upon illumination. To quench ROS production, both the cnidarian host and zooxanthellae express a full suite of antioxidant enzymes. Studying antioxidative balance is therefore crucial to understanding how symbiotic cnidarians cope with ROS production. We characterized glutathione peroxidases (GPx) in the symbiotic cnidarian Anemonia viridis by analysis of their isoform diversity, their activity distribution in the three cellular compartments (ectoderm, endoderm and zooxanthellae) and their involvement in the response to thermal stress. We identified a GPx repertoire through a phylogenetic analysis showing 7 GPx transcripts belonging to the A. viridis host and 4 GPx transcripts strongly related to Symbiodinium sp. The biochemical approach, used for the first time with a cnidarian species, allowed the identification of GPx activity in the three cellular compartments and in the animal mitochondrial fraction, and revealed a high GPx electrophoretic diversity. The symbiotic lifestyle of zooxanthellae requires more GPx activity and diversity than that of free-living species. Heat stress induced no modification of GPx activities. We highlight a high GPx diversity in A. viridis tissues by genomic and biochemical approaches. GPx activities represent an overall constitutive enzymatic pattern inherent to symbiotic lifestyle adaptation. This work allows the characterization of the GPx family in a symbiotic cnidarian and establishes a foundation for future studies of GPx in symbiotic cnidarians.
ISSN: 0300-9084

hal-01404424
http://hal.upmc.fr/hal-01404424
http://hal.upmc.fr/hal-01404424/document
http://hal.upmc.fr/hal-01404424/file/Pey_Characterization_of.pdf
DOI : 10.1016/j.biochi.2016.10.016

Éditeur(s) : HAL CCSD Springer Verlag (Germany)
Résumé : International audience
Mass bleaching events resulting in coral mortality are among the greatest threats to coral reefs, and are projected to increase in frequency and intensity with global warming. Achieving a better understanding of the consistency of the response of coral assemblages to thermal stress, both spatially and temporally, is essential to determine which reefs are more able to tolerate climate change. We compared variations in spatial and taxonomic patterns between two bleaching events at the scale of an island (Moorea Island, French Polynesia). Despite similar thermal stress and light conditions, bleaching intensity was significantly lower in 2007 (approximately 37 % of colonies showed signs of bleaching) than in 2002, when 55 % of the colonies bleached. Variations in the spatial patterns of bleaching intensity were consistent between the two events. Among nine sampling stations at three locations and three depths, the stations at which the bleaching response was lowest in 2002 were those that showed the lowest levels of bleaching in 2007. The taxonomic patterns of susceptibility to bleaching were also consistent between the two events. These findings have important implications for conservation because they indicate that corals are capable of acclimatization and/or adaptation and that, even at small spatial scales, some areas are consistently more susceptible to bleaching than others.
ISSN: 0167-6369

hal-00905944
https://hal.archives-ouvertes.fr/hal-00905944
DOI : 10.1007/s10661-012-2923-3

Éditeur(s) : HAL CCSD IOS Press
Résumé : The main objectives of the present work were to test the effects of heat stress on blood rheology and to determine whether the responses can change according to the pig breeds. Thirty-six pigs from three pig's lines (n = 12 for each line) with assumed different tolerance to heat stress were compared: Large White (LW, little tolerance), Creole (CR, good tolerance) and LW × CR pigs (produced from a cross between LW and CR lines). In a first period, all pigs were exposed to a 9-d period of thermo-neutral environment (24°C; d-9 to d-1; P0). At the end of P0, six pigs from each line were slaughtered (n = 18). Then in a second period, the remaining pigs (6/breed; n = 18) were exposed to a 5-d period of heat stress (32°C; d + 1 -d + 5; P1) and thereafter slaughtered at d + 5. Rectal and skin temperatures, as well as respiratory rate, were recorded on d-1 and d + 5. At slaughter, blood was sampled for hematological and hemorheological measurements. Heat stress caused a rise of the skin temperature and respiratory rate without any changes in the rectal temperature or on the hematological and hemorheological parameters when all pigs' lines were considered. We observed a pig line effect on blood viscosity at high shear rate (375 s-1) and red blood cell deformability at 30 Pa with CR pigs having lower blood viscosity and higher red blood cell deformability than LW pigs. While the changes of blood viscosity under heat stress did not reach statistical significance in LW and CR lines, blood viscosity (at 375 s-1) increased above the temperate values in the LW × CR line. Red blood cell deformability at 30 Pa was higher in CR pigs exposed to heat stress compared to LW pigs in the same condition. In conclusion, thermal loading caused physiological stress but did not widely change the hematological and hemorheological profiles. Although some blood rheological parameters seem to vary with the pig breeds, the responses to heat stress are very similar.
ISSN: 1386-0291

hal-01210926
https://hal.archives-ouvertes.fr/hal-01210926
DOI : 10.3233/CH-131722
PRODINRA : 286489
PUBMED : 23603327

Éditeur(s) : HAL CCSD Elsevier
Résumé : International audience
We explore the use of seismic noise produced by rivers to monitor the bed load transport in the case of a low-discharge braided river in the French Alps: the "torrent de St Pierre". For this purpose, we deployed two dedicated seismic networks during summers 2007 and 2008, for which the characteristics of the recorded continuous signal are similar despite changes in the sensor locations. For dry weather conditions, only melting of nearby glaciers controls the supply of water to the stream. In these conditions, the river hydrology and the seismic energy in the 2-80 Hz frequency band both follow a diurnal fluctuation similar to the thermal amplitude. In contrast during rainfall episodes, the temperature variation fails to explain the hydrodynamic changes. Dense cloud covers reduce glacier melting and the recorded seismic energy denotes bursts of high-frequency seismic noise well correlated with water level data. Comparisons between the recorded seismic signals and the collected hydrological and sediment load data indicate that a frequency band of 3-9 Hz best explains the water level changes and thus the seismic waves coming from the flow turbulence. These analyses also reveal the presence of a seismic noise threshold that might be linked to the water shear stress exerted by the flowing water. Using the seismic energy in this frequency band as a proxy of the fluvial shear stress, the seismic-hydrologic relationship may be sensitive to variations in bed load transport. The spectral content of the seismic energy shows patterns consistent with the mobilization of sediment particles. From the interpretations of the seismic wave attenuation of river sources, we finally propose that stations at a distance from the stream less than 50 m are able to record most sediment particles. Farther stations are still useful during extreme events when largest grain sizes are mobilized. More generally this study demonstrates the feasibility of using the river seismic signal to survey bed load transport in various river types from small braided mountain rivers like the "torrent de St Pierre" to the large entrenched Himalayan rivers.
ISSN: 0022-1694

hal-00644789
https://hal.archives-ouvertes.fr/hal-00644789
DOI : 10.1016/j.jhydrol.2011.07.014

Éditeur(s) : HAL CCSD
Résumé : The Deep Basin of the Gulf of Mexico (DBGM) extends over eastern Mexico, the southeastern part of the United States, west of the Atlantic Ocean. This study, based on the interpretation and integration of seismic profiles, exploration wells and outcrop studies, focus on the deep part of the Gulf of Mexico, where bathymetry varies from 200 to 3750 m, thus comprising both the continental slope and the abyssal plain. The first part of this thesis focus on the description of the sedimentary infill of the western part of the Gulf of Mexico, in the Veracruz State, and to its geodynamic controls. The geodynamic evolution of the Deep Basin of the Gulf of Mexico (DBGM) begins during the Triassic-Jurassic with the break-up and the opening of a continental rift, in the southern part of the North American lithospheric plate. This opening induced a relative movement of the Yucatan Block towards the southeast. This intra-continental rifting episode was followed by a stage of post-rift thermal subsidence in the basins of the continental margin in the west, coeval with oceanic accretion in the DBGM. The thermal subsidence of the margin was subsequently modified by the Laramian orogeny, which impacted strongly the overall architecture of the margin as well as its litho-stratigraphic evolution, inducing the deposition of siliciclastic deposits in various morphotectonic provinces: i.e., near the tectonic front of the Sierra Madre Oriental (SMO), within the adjacent Chicontepec foreland basin, over the Tuxpan Platform (Golden Lane), across the continental slope and up to the deep abyssal plain, these two last morphotectonic provinces belonging to the DBGM. During the Early Paleogene, the effects of the thermal subsidence of the passive margin were stressed by the tectonic load of the Laramian orogen (i.e., the Sierra Madre Oriental, SMO), thus allowing the development of a foreland basin flexural. In this geodynamic framework, the main sedimentary transfers developed from the tectonic front "SMO" in the west, towards the DBGM in the east, the main source for clastic sediments being linked to the erosion of the "SMO" mountains. During the Paleocene and the Early Eocene, the architecture of the silici-clastic syn-tectonic sediments deposited in submarine fans was characterized by sliding, turbidites with A and B Bouma facies, as well as levees and channels. After the stop of the flexural subsidence, the thermal subsidence of the passive margin resumed during the Late Eocene, the Oligocene and the Neogene, allowing the development of a new sedimentary prism, prograding eastwards toward the DBGM. This sedimentary infill was again made up of levees-channels, sand bars and delta systems. During the Neogene, an extensional system with listric faults and roll-over features developed across the slope of the DBGM, due to an active detachment developing within overpressured Eocene-Oligocene clays. This gravitational gliding of Neogene series accounted also for the development of compressional features at the toe of the slope. Approximately 60% of the Miocene siliciclastic sediments have been trapped in growth strata and slope basins associated with this complex gravitational system, ranging from river-delta features towards gravity slides associated with slump facies. The second part of this thesis aims at a quantification of these various processes, including the construction of balanced cross sections, forward Thrustpack kinematic modelling coupling the development of a basal detachment, lithospheric flexure, erosion and sedimentation, as well as subsequent stratigraphic modelling with the Dionisos software, the later aiming at predicting the sand versus clay ratios in Neogene siliciclastic deposits of the DBGM and its surroundings.
Le bassin profond du Golfe du Mexique (BPMG) est localisé à l'est du Mexique, au sud-est des États-Unis et à l'ouest de l'Océan Atlantique. Cette étude de la partie profonde du Golfe du Mexique est basée sur l'intégration de données de sismique, de forages pétroliers et d'études de terrain; elle comprend toute la pente continentale et la plaine abyssale, avec une bathymétrie qui varie de 200 à 3750 m. La première partie de cette thèse est consacrée à la description du remplissage sédimentaire de la bordure occidentale du Golfe du Mexique, dans le secteur de Veracruz, en liaison avec son évolution géodynamique. L'évolution géodynamique du BPMG commence au Trias-Jurassique avec la rupture et la propagation d'un rift continental, dans le secteur sud de la plaque nord américaine. Cette ouverture et le déplacement relatif vers le sud-est du bloc crustal du Yucatan sont à l'origine du BPGM. Cette géodynamique de rift continental est suivie d'une étape post-rift accompagnée de l'océanisation du bassin. Les bassins de la marge passive ont poursuivi leur évolution sous l'effet de la subsidence thermique à l'ouest du Golfe du Mexique, tandis que de la croûte océanique se formait dans le BPGM. Cette subsidence thermique de la marge a ensuite été perturbée par l'orogénèse Laramienne, qui a remodelé l'architecture stratigraphique silico-clastique des dépôts du Tertiaire entre les éléments morphotectoniques suivants: lefront tectonique de la Sierra Madre Orientale (SMO), le bassin d'avant-pays Chicontepec, la Plateforme de Tuxpan-Faja de Oro, la pente continentale et la plaine abyssale, ces deux dernières provinces morphotectoniques appartenant au BPGM. Pendant le Paléogèneinférieur, les effets de la subsidence thermique de la marge passive ont été accentués par la charge tectonique de l'orogénèse laramienne (SMO), permettant ainsi le développement d'un bassin flexural d'avant-pays. Au cours de cette étape, les principaux transferts sédimentaires se sont effectués du front tectonique "SMO" vers le BPGM. La source principale de sédiments clastiques est liée à l'érosion de la chaîne de montagnes "SMO". Pendant le Paléocène et l'Éocène inférieur, l'architecture des premiers sédiments silico-clastiques syn-tectoniques déposés dans des éventails sous-marins sont caractérisés par des figures de glissement, des faciès turbiditiques A et B de Bouma, des chenaux-levées. Après l'arrêt de la subsidence flexurale, la subsidence thermique de la marge passive s'est poursuivie pendant l'Éocène supérieur, l'Oligocène et le Néogène, permettant le développement d'un nouveau prisme sédimentaire progradant. Les remplissages sédimentaires sont encore constitués de chenaux et de levées, avec des barres de sable associées à des systèmes deltaïques sur la plateforme. Pendant le Néogène, un système de failles listriques s'est développé sur la pente du BPGM, au-dessus d'une surface de décollement située, dans la région d'étude, dans les argiles de l'Éocène-Oligocène. Ce système de failles de croissance a piégé plus de 60% des sédiments silico-clastiques du Miocène. Ce remplissage sédimentaire évolue latéralement de faciès fluviaux deltaïques vers des faciès de pente affectés de glissements gravitaires et associés à des turbidites. La deuxième partie de cette thèse est consacrée à une approche quantitative basée sur des modélisations structurales (coupes équilibrées et modélisations cinématiques directes avec Thrustpack, couplant décollement gravitaire, flexure lithosphérique, érosion et sédimentation), puis sédimentaires (prise en compte des transferts de matériel clastique depuis la partie émergée de la chaîne jusqu'au bassin profond, à l'aide du logiciel Dionisos, afin de mieux comprendre les processus de piégeage des sédiments grossiers dans les structures de croissance et les bassins perchés de la marge.
https://tel.archives-ouvertes.fr/tel-00435120

tel-00435120
https://tel.archives-ouvertes.fr/tel-00435120
https://tel.archives-ouvertes.fr/tel-00435120/document
https://tel.archives-ouvertes.fr/tel-00435120/file/thesealzaga.pdf

Éditeur(s) : HAL CCSD Elsevier
Résumé : International audience
Rapid cold hardening (RCH) is a form of thermal acclimation that allows ectotherms to fine-tune their physiological state to match rapid changes in thermal environment. Despite progress in recent years, there is still a considerable uncertainty regarding the physiological basis of RCH in insects. Here we investigated the physiological response of adult Drosophila melanogaster to a gradual reduction of temperature from 25 to 0°C followed by 1h at 0°C. As expected, this RCH treatment promoted cold tolerance, and so we hypothesized that this change could be detected at the proteomic level. Using 2D-DIGE, we found that only a few proteins significantly changed in abundance, and of these, we identified a set of four proteins of particular interest. These were identified as two different variants of glycogen phosphorylase (GlyP) of which three spots were up-regulated and another was down regulated. In subsequent experiments, we quantified upstream events by measuring the GlyP mRNA amount, but we found no marked effect of RCH. We also examined downstream events by measuring GlyP activity and the level of free sugars. We found no effect of RCH on GlyP activity. On the other hand, screening of whole animal sugar contents revealed a small increase in glucose levels following RCH while trehalose content was unaltered. This study highlights a complex regulation of GlyP in relation to RCH where we found associations between the cold tolerance, the protein abundance and the metabolite concentrations but no changes in mRNA expression and enzyme activity. These data stress the necessity of combining the hypothesis-generating power of an 'Omics' approach with subsequent targeted validations across several levels of the biological organization. We discuss reasons why different biological linked levels do not necessarily change stoichiometrically.
ISSN: 0022-1910

hal-00991032
https://hal-univ-rennes1.archives-ouvertes.fr/hal-00991032
DOI : 10.1016/j.jinsphys.2014.01.009
PUBMED : 24508557

Éditeur(s) : Public Library Science
Résumé : Global change and its associated temperature increase has directly or indirectly changed the distributions of hosts and pathogens, and has affected host immunity, pathogen virulence and growth rates. This has resulted in increased disease in natural plant and animal populations worldwide, including scleractinian corals. While the effects of temperature increase on immunity and pathogen virulence have been clearly identified, their interaction, synergy and relative weight during pathogenesis remain poorly documented. We investigated these phenomena in the interaction between the coral Pocillopora damicornis and the bacterium Vibrio coralliilyticus, for which the infection process is temperature-dependent. We developed an experimental model that enabled unraveling the effects of thermal stress, and virulence vs. non-virulence of the bacterium. The physiological impacts of various treatments were quantified at the transcriptome level using a combination of RNA sequencing and targeted approaches. The results showed that thermal stress triggered a general weakening of the coral, making it more prone to infection, non-virulent bacterium induced an 'efficient' immune response, whereas virulent bacterium caused immuno-suppression in its host.
Plos One (1932-6203) (Public Library Science), 2014-09 , Vol. 9 , N. 9 , P. 1-15

Droits : This is an open-access article, free of all copyright, and may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose. The work is made available under the Creative Commons CC0 public domain dedication.
http://archimer.ifremer.fr/doc/00221/33265/31738.pdf
DOI:10.1371/journal.pone.0107672
http://archimer.ifremer.fr/doc/00221/33265/

Éditeur(s) : HAL CCSD Elsevier
Résumé : International audience
Structural controls are critical during magmatic-to-hydrothermal transition in the formation of intrusion-related gold deposits. They may explain why only some parts of intrusions are mineralized and why only very few intrusions host large deposits. Moreover, most of these gold deposits postdate peak regional metamorphism and were formed in zones of relatively low strain. Indeed, the efficiency of structural gold traps is highest along favourably oriented intrusion/host-rock contacts where mechanical instability maintains high permeability in the cracked thermal aureole. This may be reinforced by melt injections from an underlying root zone. We present a structural analysis of the deformation features of a granite-pluton roof; large-scale dykes and a network of gold veins are intensively developed in this roof, recording a succession of mechanical instabilities. Our gravity survey underlines the presence of a pluton feeder zone located just beneath the mineralized network. It is argued that interferences between regional stress and melt injection in the feeder zone favoured the development of the network by strain located close to the granite roof. This stresses the role of mechanical instabilities triggered by the combined effects of regional stress and melt dynamics in determining the location and size of this type of gold deposit.
ISSN: 0191-8141

insu-00914780
https://hal-insu.archives-ouvertes.fr/insu-00914780
https://hal-insu.archives-ouvertes.fr/insu-00914780/document
https://hal-insu.archives-ouvertes.fr/insu-00914780/file/manuscript.pdf
DOI : 10.1016/j.jsg.2013.11.006

Éditeur(s) : HAL CCSD Public Library of Science
Résumé : Heat stress is one of the major limiting factors of production efficiency in the swine industry. The aims of the present study were 1) to observe if hemorheological and hematological parameters could be associated to physiological acclimation during the first days of heat stress exposure and 2) to determine if water restriction could modulate the effect of thermal heat stress on physiological, hematological and hemorheological parameters. Twelve Large White male pigs were divided into an ad libitum and a water restricted group. All pigs were submitted to one week at 24 °C (D-7 to D-1). Then, at D0, temperature was progressively increased until 32 °C and maintained during one week (D1 to D7). We performed daily measurements of water and feed intake. Physiological (i.e., skin temperature, rectal temperature, respiratory rate), hematological and hemorheological parameters were measured on D-6, D-5, D0, D1, D2 and D7. Water restriction had no effect on physiological, hematological and hemorheological parameters. The first days of heat stress caused an increase in the three physiological parameters followed by a reduction of these parameters suggesting a successful acclimation of pigs to heat stress. We showed an increase in hematocrit, red blood cell aggregation and red blood cell aggregation strength during heat stress. Further, we observed an important release of reticulocytes, an increase of red blood cell deformability and a reduction of feed intake and blood viscosity under heat stress. This study suggests that physiological acute adaptation to heat stress is accompanied by large hematological and hemorheological changes.
ISSN: 1932-6203

hal-01210648
https://hal.archives-ouvertes.fr/hal-01210648
DOI : 10.1371/journal.pone.0102537
PRODINRA : 269557
PUBMED : 25013960

Éditeur(s) : HAL CCSD Public Library of Science
Résumé : International audience
Purpose: This study compares the effects of neutral temperature, cold and ice-slush beverages, with and without 0.5% menthol on cycling performance, core temperature (T co) and stress responses in a tropical climate (hot and humid conditions). Methods: Twelve trained male cyclists/triathletes completed six 20-km exercise trials against the clock in 30.7uC60.8uC and 78%60.03% relative humidity. Before and after warm-up, and before exercise and every 5 km during exercise, athletes drank 190 mL of either aromatized (i.e., with 0.5 mL of menthol (5 gr/L)) or a non-aromatized beverage (neutral temperature: 23uC60.1uC, cold: 3uC60.1uC, or ice-slush: 21uC60.7uC). During the trials, heart rate (HR) was continuously monitored, whereas core temperature (T co), thermal comfort (TC), thermal sensation (TS) and rate of perceived exertion (RPE) were measured before and after warm-up, every 5 km of exercise, and at the end of exercise and after recovery. Results: Both the beverage aroma (P,0.02) and beverage temperature (P,0.02) had significant and positive effects on performance, which was considerably better with ice-slush than with a neutral temperature beverage, whatever the aroma (P,0.002), and with menthol vs non-menthol (P,0.02). The best performances were obtained with ice-slush/menthol and cold/menthol, as opposed to neutral/menthol. No differences were noted in HR and T co between trials. Conclusion: Cold water or ice-slush with menthol aroma seems to be the most effective beverage for endurance exercise in a tropical climate. Further studies are needed to explore its effects in field competition.
ISSN: 1932-6203

hal-01135125
https://hal.univ-antilles.fr/hal-01135125
https://hal.univ-antilles.fr/hal-01135125/document
https://hal.univ-antilles.fr/hal-01135125/file/Riera%20et%20al.%20-%202014%20-%20Physical%20and%20perceptual%20cooling%20with%20beverages%20to%20increase%20cycle%20performance%20in%20a%20tropical%20climate%284%29.pdf
DOI : 10.1371/journal.pone.0103718

Éditeur(s) : HAL CCSD Elsevier Masson
Résumé : International audience
The seismological study of recent seismic crises near Oleron Island confirms the coexistence of an extensional deformation and a transtensive regime in the Atlantic margin of France, which is different from the general western European stress field corresponding to a strike-slip regime. We argue that the switch of the principal stress axes σ1/σ2 in a NW-SE vertical plane is linked with the existence of crustal heterogeneities. Events of magnitude larger than 5 sometimes occur along the Atlantic margin of France, such as the 7 September 1972 (ML = 5.2) earthquake near Oleron island and the 30 September 2002 (ML = 5.7) Hennebont event in Brittany. To test the mechanism of local strain localization, we model the deformation of the hypocentral area of the Hennebont earthquake using a 3D thermo-mechanical finite element code. We conclude that the occurrence of moderate earthquakes located in limited parts of the Hercynian shear zones (as the often reactivated swarms near Oleron) could be due to local reactivation of pre-existing faults. These sporadic seismic ruptures are favoured by stress concentration due to rheological heterogeneities.
ISSN: 1631-0713

hal-00936220
https://hal.archives-ouvertes.fr/hal-00936220
DOI : 10.1016/j.crte.2013.07.004

Éditeur(s) : HAL CCSD Royal Society
Résumé : Coral bleaching events are predicted to occur more frequently in the coming decades with global warming. The susceptibility of corals to bleaching during thermal stress episodes depends on many factors, including the magnitude of thermal stress and irradiance. The interactions among these two factors, and in particular with ultra-violet radiation (UVR), the most harmful component of light, are more complex than assumed, and are not yet well understood. This paper explores the individual and combined effects of temperature and UVR on the metabolism of Acropora muricata, one of the most abundant coral species worldwide. Particulate and dissolved organic matter (POM/DOM) fluxes and organic matter (OM) degradation by the mucus-associated bacteria were also monitored in all conditions. The results show that UVR exposure exacerbated the temperature-induced bleaching, but did not affect OM fluxes, which were only altered by seawater warming. Temperature increase induced a shift from POM release and DOM uptake in healthy corals to POM uptake and DOM release in stressed ones. POM uptake was linked to a significant grazing of pico- and nanoplankton particles during the incubation, to fulfil the energetic requirements of A. muricata in the absence of autotrophy. Finally, OM degradation by mucus-associated bacterial activity was unaffected by UVR exposure, but significantly increased under high temperature. Altogether, our results demonstrate that seawater warming and UVR not only affect coral physiology, but also the way corals interact with the surrounding seawater, with potential consequences for coral reef biogeochemical cycles and food webs.
ISSN: 2046-6390

hal-01606454
https://hal.archives-ouvertes.fr/hal-01606454
DOI : 10.1242/bio.026757
PRODINRA : 406580
PUBMED : 28811302

Éditeur(s) : HAL CCSD
Résumé : Several major earthquakes have struck the 2500 km long Himalayan range during the past century. However, both the maximum size of such earthquakes and the probability of occurrence of a magnitude 9 megaquake are still a mater of debate. In this framework it is of key importance to investigate the lateral segmentation of the Himalayan arc in order to deepen our understanding of the mechanisms that control such events as well as their extents. Besides the remarkably uniform tectonic set-up and the fact that most studies on the Himalayas have focused on Central Nepal, several indications of along strike changes can be gathered.This thesis brings new constraints on lateral variations of crustal structure and of deformation between western Nepal (~80°E) and eastern Bhutan (~92°E).The first part of this thesis focuses on the lateral variations of crustal structure. In the past three years we have carried out four land gravity campaigns. We have established 366 new gravity measurement points to fill the data gaps in central and western Nepal as well as in Bhutan. This new dataset, together with available seismological data, is used to constrain thermo-mechanical models of the bending of the India plate underneath the Himalayas. While the inferred crustal geometry does not exhibit major along-strike variations over central and eastern Nepal, the shorter wavelength flexure of the lithosphere in Bhutan is associated with a weaker upper mantle rheology.The second part focuses on the lateral variations of recent deformation in the Himalayas. Fifteen years of paleoseismological investigations have extended the catalogue of major Himalayan events over the last millennium. Combining these information with secular loading, we assess the spatial and temporal stress changes on the Main Himalayan Thrust along the orogen over the last nine centuries. Our calculations indicate that inter-seismic loading has now nearly overcome the Coulomb stress decrease caused by the great ~1100 medieval earthquake. Our results also point out that the 1897 Shillong plateau earthquake does not have a major influence on the stress accumulated on the Main Himalayan Thrust since then. In order to better characterize active tectonics in the Bhutan Himalayas where no studies were done so far, we carried out a morphotectonic analysis in the south-central part of the kingdom. We show that the same amount of Holocene deformation is accommodated on the frontal thrust in Bhutan as on the neighbouring portions of the Himalayan arc. We also find evidences for two major (M>8) earthquakes on this thrust in the Bhutan Himalayas during the last millennium. Our results therefore show that Bhutan cannot be considered as a seismic gap. They also challenge the interpretation of one single ~1100 medieval mega-event that would have ruptured the Main Frontal Thrust from central Nepal to eastern Bhutan.
Au cours du dernier siècle, plusieurs séismes majeurs ont affecté l'Himalaya. Cependant, la taille maximale de ces événements et la probabilité d'occurrence de méga-séismes avec des magnitudes proches de 9 sont toujours matière à débat. L'étude de la segmentation de l'arc Himalayen est donc primordiale afin de comprendre les mécanismes qui contrôlent ces séismes ainsi que leur extension spatiale. La compréhension du cycle sismique en Himalaya est aujourd'hui essentiellement basée sur des études menées au Népal central, ce qui limite notre connaissance de son fonctionnement tri-dimensionnel. Ce travail de thèse permet d'apporter de nouvelles contraintes sur les variations latérales de la déformation crustale dans la zone comprise entre l'ouest Népal (~80°E) et l'est Bhoutan (~92°E). La première partie de cette thèse est consacrée à l'étude des variations latérales de la structure lithosphérique. Quatre campagnes gravimétriques ont été réalisées entre 2010 et 2012. Elles ont permis d'étendre le réseau gravimétrique Népalais jusqu'à l'ouest Népal et d'établir le premier réseau gravimétrique Bhoutanais. Ce nouveau jeu de données, combiné avec les données sismologiques disponibles, permet de contraindre des modèles thermo-mécaniques de la flexure de la plaque Indienne sous l'Himalaya. Les résultats suggèrent qu'il n'existe pas de variations latérales majeures du comportement mécanique de la lithosphère Indienne entre le centre et l'est Népal contrairement au Bhoutan où une rigidité flexurale plus faible est nécessaire pour expliquer les données.La deuxième partie de cette thèse est consacrée à l'étude des variations latérales des déformations récentes dans le prisme Himalayen. Les études paléosismologiques menées depuis 15 ans en Himalaya ont permis d'étendre le catalogue des événements majeurs sur le dernier millénaire. En intégrant à la fois les contraintes disponibles sur ces séismes majeurs et le chargement séculaire, nous étudions les variations spatiales et temporelles de l'état de contrainte du chevauchemlent Himalayen principal le long de l'arc. Nos calculs montrent que même dans la cas où un méga-séisme se serait produit en ~1100 en Himalaya, le chargement séculaire a quasiment compensé la chute de contrainte associée. Les modèles montrent aussi que le séisme du Shillong ne joue pas un rôle majeur sur les contraintes accumulées sur le chevauchement Himalayen depuis 1897 au niveau du Bhoutan. Enfin, nous présentons les premières contraintes sur la tectonique active au Bhoutan. L'étude morphotectonique réalisée au centre du Sud-Bhoutan a permis de montrer que la majeure partie de la déformation Holocène est accommodée au niveau du front Himalayen, comme au Népal. Nous montrons aussi des évidences de ruptures de surface avec des décalages verticaux de plusieurs mètres associés à deux séismes majeurs sur le dernier millénaire. Ces évidences remettent en cause l'interprétation d'un méga-séisme en ~1100.
https://tel.archives-ouvertes.fr/tel-01002381

NNT : 2013MON20074
tel-01002381
https://tel.archives-ouvertes.fr/tel-01002381
https://tel.archives-ouvertes.fr/tel-01002381/document
https://tel.archives-ouvertes.fr/tel-01002381/file/35970_BERTHET_2013_archivage_cor.pdf

Éditeur(s) : HAL CCSD Wiley
Résumé : International audience
Oxidative stress and haemolysis-associated nitric oxide (NO) depletion plays a crucial role in the development of vasculopathy in sickle cell anaemia (SS). However it remains unknown whether oxidative stress and haemolysis levels influence vascular function in patients with sickle haemoglobin C disease (SC). Microvascular response to heat (using Laser Doppler flowmetry on finger), oxidative stress biomarkers, NO metabolites, endothelin-1 and haematological parameters were compared between patients with SS and SC. Vascular function, oxidative and nitrosative markers were also measured in healthy (AA) children. SS and SC had increased plasma advanced oxidation protein products (AOPP), malondialdehyde, plasma antioxidant activities and NO end products, compared to AA. SC had lower catalase activity compared to AA and SS. Haemolytic rate, glutathione peroxidase and nitrotyrosine concentrations were significantly increased in children with SS compared to SC and AA. SS and SC had impaired microvascular reactivity compared to AA. In SS, the plateau phase of the response to local thermal heating was negatively associated with nitrotyrosine and AOPP. No association between vascular function parameters and oxidative stress markers was observed in SC. Mild haemolysis in SC, compared to SS, may limit oxidative and nitrosative stress and could explain the better preserved microvascular function in this group.
ISSN: 0007-1048

hal-01668314
https://hal.univ-antilles.fr/hal-01668314
DOI : 10.1111/bjh.14693

Éditeur(s) : HAL CCSD
Résumé : International audience
As subducting plates reach the base of the upper mantle, some appear to flatten and stagnate, while others seemingly go through unimpeded. This variable resistance to slab sinking has been proposed to affect long-term thermal and chemical mantle circulation. A review of observational constraints and dynamic models highlights that neither the increase in viscosity between upper and lower mantle (likely by a factor 20–50) nor the coincident endothermic phase transition in the main mantle silicates (with a likely Clapeyron slope of –1 to –2 MPa/K) suffice to stagnate slabs. However, together the two provide enough resistance to temporarily stagnate subducting plates, if they subduct accompanied by significant trench retreat. Older, stronger plates are more capable of inducing trench retreat, explaining why backarc spreading and flat slabs tend to be associated with old-plate subduction. Slab viscosities that are ∼2 orders of magnitude higher than background mantle (effective yield stresses of 100–300 MPa) lead to similar styles of deformation as those revealed by seismic tomography and slab earthquakes. None of the current transition-zone slabs seem to have stagnated there more than 60 m.y. Since modeled slab destabilization takes more than 100 m.y., lower-mantle entry is apparently usually triggered (e.g., by changes in plate buoyancy). Many of the complex morphologies of lower-mantle slabs can be the result of sinking and subsequent deformation of originally stagnated slabs, which can retain flat morphologies in the top of the lower mantle, fold as they sink deeper, and eventually form bulky shapes in the deep mantle.
ISSN: 1553-040X

hal-01571965
https://hal.archives-ouvertes.fr/hal-01571965
DOI : 10.1130/GES01476.1