Éditeur(s) : HAL CCSD
Résumé : International audience
STUDY QUESTION: Do freezing and in vitro culture procedures enhance the expression of proteins involved in apoptotic or autophagic pathways in murine pre-pubertal testicular tissue? SUMMARY ANSWER: IVM strongly modified apoptosis- and autophagy-related relative protein levels in mice testicular tissue whereas the impact of cryopreservation procedures was minimal at the end of the culture. WHAT IS KNOWN ALREADY: In vitro spermatogenesis remains a challenging technical issue as it imposes to find a very close balance between survival and death of germ cell natural precursors (i.e. gonocytes and spermatogonia), which will eventually undergo a complete spermatogenesis close to in vivo conditions. The establishment of efficient culture conditions coupled with suitable cryopreservation procedures (e.g. controlled slow freezing [CSF] and solid surface vitrification [SSV]) of pre-pubertal testicular tissue is a crucial step in the fields of fertility preservation and restoration to improve the spermatic yield obtained in vitro. STUDY DESIGN, SIZE, DURATION: Here, we study cryopreservation procedures (i.e. CSF or SSV) and the impact of culture media compositions. A first set of 66 mouse pre-pubertal testes were directly cultured during 30, 36, 38 and 60 days (D) from 2.5 to 6.5-day-old CD-1 mice to evaluate the impact of time-aspect of culture and to endorse the reverse phase protein microarrays (RPPM) technique as an adapted experimental tool for the field of in vitro spermatogenesis. Ninety others fresh, slow-frozen and vitrified pre-pubertal testes were cultured during 30 days for the principal study to evaluate the impact of cryopreservation procedures before and after culture. Thirty-four testes dissected from 2.5, 6.5, 36.5, 40.5, 42.5 and 62.5 days postpartum (dpp) mice, corresponding to the time frames of spermatogenesis orchestrated in vitro, were used as in vivo controls. PARTICIPANTS/MATERIALS, SETTING, METHODS: After in vitro culture, testicular tissue samples originated from 2.5 or 6.5-day-old CD-1 male mice were analyzed using RPPM. This targeted proteomic technique allowed us to assess the expression level of 29 apoptosis- and autophagy-related factors by normalizing blank-corrected signal values. In addition, morphological analyses (e.g. HES, PAS, TRA98 and CREM) and DNA fragmentation in intra-tubular cells (i.e. terminal deoxynucleotidyl transferase dUTP nick end labeling; TUNEL) were assessed for the distinct experimental conditions tested as well as for in vivo control mouse testes. MAIN RESULTS AND THE ROLE OF CHANCE: A validation of the RPPM procedure in the field of in vitro spermatogenesis was completed with assay and array robustness before a principal study concerning the evaluation of the impact of in vitro culture and cryopreservation procedures. The proportion of elongated spermatids and the total cell number per seminiferous tubule tended to be very different between the in vivo and in vitro conditions (P < 0.05), suggesting the presence of a beneficial regulation on the first spermatogenesis wave by intrinsic apoptosis (Caspase₉) and autophagy (Atg5) factors (P < 0.0003 and r2 = 0.74). Concerning the impact of culture media compositions, a basic medium (BM) composed of αMEM plus 10% KnockOut™ serum replacement and gentamicin supplemented with retinol (Rol) and vitamin E (Vit. E) was selected as the best culture medium for fresh 6.5 dpp tissue cultured during 30D with 27.7 ± 8.10% of seminiferous tubules containing elongated spermatids. Concerning the impact of cryopreservation procedures, SSV did not have any impact on the morphological parameters evaluated after culture in comparison to fresh tissue (FT) controls. The proportion of tubules with elongated spermatids on testicular explants cultured with BMRol+Vit. E was not different between SSV (6.6 ± 1.6%) and CSF (5.3 ± 1.9%); however, round spermatids were observed more frequently for SSV (19 ± 6.2%) than CSF (3.3 ± 1.9%, P = 0.0317). Even if the proportion of TUNEL-positive cells for BMRol+Vit. E was higher at D30 after SSV (4.12 ± 0.26%) than CSF (1.86 ± 0.12%, P = 0.0022) and FT (2.69 ± 0.33%, P = 0.0108), the DNA damages observed at the end of the culture (i.e. D30) were similar to respective 6.5 dpp controls. In addition, the relative protein level expression ratio of an apoptotic factor, the phosphorylated FADD on Fas, was reduced by 64-fold in vitrified testes cultured with BMRol+Vit. E. Furthermore, we found in this study that the StemPro®-34 SFM culture medium supplemented with growth factors (e.g. EGF, bFGF, GDNF and LIF) prevented the differentiation of spermatogonial stem cells in favor of a significant proliferation with a better architectural pattern than in vivo 6.5 dpp controls with an increase of seminiferous tubules area for FT (P = 0.0357) and CSF (P = 0.0317). LIMITATIONS REASONS FOR CAUTION: Despite our promising results, the evaluation of apoptotic- and autophagic-related proteins was studied for a limited amount of proteins and on global testicular tissue. WIDER IMPLICATIONS OF THE FINDINGS: The data presented herein will help to improve apoptotic and autophagic understanding during the first spermatogenic wave. Moreover, our findings illustrate for the first time that, using finely-tuned experimental conditions, a testicular in vitro culture combined with proteomic technologies may significantly facilitate the study of cryopreservation procedures and in vitro culture evaluations. This study may also contribute to improve work on testicular tissues from pre-pubertal and adolescent cancer survivors. STUDY FUNDING/COMPETING INTEREST(S): This study was supported by a Ph.D. grant from the Rouen Normandie Université and a financial support from 'la Ligue nationale contre le cancer' (both awarded to L.D.), funding from Institute for Research and Innovation in Biomedicine (IRIB), Agence de la Biomédecine, and co-supported by European Union and Région Normandie. Europe gets involved in Normandie with European Regional Development Fund (ERDF). The authors declare that there is no conflict of interest.
Molecular Human Reproduction

hal-01647081
https://hal-univ-rennes1.archives-ouvertes.fr/hal-01647081
DOI : 10.1093/molehr/gax054
PUBMED : 29040674

Éditeur(s) : Université de Bretagne Occidentale
Résumé : Study of mineral suspended particulate matter (SPM) is essential to understand ecosystem dynamics. SPM influences light penetration which is closely related to primary production and carries pollutants and nutrients within the water masses. This work aims to study near-surface mineral SPM dynamics in the English Channel using two complementary methods: remote-sensing by satellites and numerical modelling. Firstly, MODIS and MERIS satellite images processed by the IFREMER semi-analytical algorithm and describing non-algal SPM, are analysed to build three statistical models which are able to estimate near-surface SPM using simple variables, e.g., tidal coefficient, significant wave height and mean chlorophyll-a concentration. Secondly, the three-dimensional hydrosedimentary model ROMS (Regional Ocean Modeling System) is applied in the English Channel. It considers tidal forcing along open boundaries, wind stress at the sea-surface, wave-current interactions in the bottom boundary layer and realistic heterogeneous bottom sediments. Results of the more sophisticated statistical model is also used to impose SPM concentrations at open boundaries. Sensitivity tests on SPM boundary conditions show the importance of prescribing realistic sediment concentrations to improve the model predictions. Biological influences on mineral SPM are also tested using settling velocity and critical shear stress for erosion which vary with seasons. Both statistical and numerical models highlight and locate respective influences of tide, waves and biology on mineral SPM. Numerical predictions indicate that near-surface SPM is mainly composed of silts (>70%). Near-surface suspended silts come largely from seabed resuspension alongshore in the Eastern Channel and from the open boundaries of our limited-area coastal model at the West of Cotentin Peninsula and offshore. Near-surface SPM variations are investigated at semi-diurnal, neap-spring tidal cycle time scales and during a wave event. Finally, dynamics of sediments in the turbidity maximum zone around the Isle of Wight is examined depending on hydrodynamical conditions in order to distinguish the role played by advection and local erosion/settling processes in this area. Keywords: Suspended particulate matter, satellite, remote-sensing, modelling, English Channel, sediments, turbidity, tide, wave
L'étude des matières en suspension (MES) minérales est essentielle pour comprendre le fonctionnement des écosystèmes en Manche : les MES influencent la pénétration de la lumière dans la colonne d'eau, paramètre clé pour la production biologique, et sont susceptibles de transporter des polluants et nutriments. Lors de ce travail, la dynamique des MES minérales de surface en Manche est étudiée à l'aide de deux méthodes complémentaires : l'observation par satellite et la modélisation numérique tridimensionnelle. Dans un premier temps, les images satellite MODIS et MERIS, traitées par l'algorithme semi-analytique développé par l'IFREMER et représentant les MES non-algales de surface, sont analysées afin d'élaborer trois modèles statistiques permettant d'estimer la MES de surface en fonction de variables basiques telles que le coefficient de marée, la hauteur significative des vagues et la concentration moyenne en chlorophylle-a. Dans un deuxième temps, le modèle hydrosédimentaire multiclasse tridimensionnel ROMS (Regional Ocean Modeling System) est implémenté en Manche. Il prend en considération le forçage par la marée aux frontières ouvertes, la contrainte exercée par le vent à la surface de la mer, l'interaction houle-courant en couche limite de fond et l’hétérogénéité spatiale réaliste du lit sédimentaire. Les résultats du modèle statistique le plus sophistiqué sont également utilisés pour forcer la concentration en MES aux frontières ouvertes du domaine de calcul. Des tests de sensibilité sur les conditions limites montrent l'importance de considérer de manière réaliste les sédiments entrant en Manche par les frontières ouvertes lors de la modélisation numérique. L'influence de la biologie sur les MES minérales est testée en utilisant une formulation de la vitesse de chute et de la contrainte critique d'érosion variant saisonnièrement. Grâce à ces deux outils associés, les influences respectives de la marée, de la houle et de la biologie sur les MES minérales sont mises en avant et localisées en Manche. Le modèle numérique prédit que les MES de surface sont composées principalement de silts (>70%). Les silts en suspension en surface proviennent majoritairement du lit sédimentaire près des côtes en Manche orientale et des frontières ouvertes du domaine de calcul à l'ouest de la presqu'île du Cotentin et au large en Manche centrale et orientale. Les variations des MES de surface sont ensuite étudiées aux échelles des cycles de marée semi-diurnes, vive-eau/morte-eau et autour d'un épisode de houles. Enfin, l'évolution de la concentration de sédiments dans la zone de forte turbidité autour de l'île de Wight peut être analysée suivant les conditions hydrodynamiques afin d'évaluer les rôles respectifs des processus locaux d'érosion/dépot et d'advection dans la génération de ces turbidités remarquables. Mots-clés : Matière en suspension, télédétection, satellite, modélisation, Manche, sédiments, turbidité, marée, houle

Droits : 2013 UBO
http://archimer.ifremer.fr/doc/00157/26783/24884.pdf
http://archimer.ifremer.fr/doc/00157/26783/

Éditeur(s) : HAL CCSD European Geosciences Union
Résumé : International audience
We calculated the seismic properties of 47 mantle xenoliths from 9 kimberlitic pipes in the Kaapvaal craton based on their modal composition, the crystal-preferred orientations (CPO) of olivine, ortho- and clinopyroxene, and garnet, the Fe content of olivine, and the pressures and temperatures at which the rocks were equilibrated. These data allow constraining the variation of seismic anisotropy and velocities within the cratonic mantle. The fastest P and S2 wave propagation directions and the polarization of fast split shear waves (S1) are always subparallel to olivine [100] axes of maximum concentration, which marks the lineation (fossil flow direction). Seismic anisotropy is higher for high olivine contents and stronger CPO. Maximum P wave azimuthal anisotropy (AVp) ranges between 2.5 and 10.2% and the maximum S wave polarization anisotropy (AVs), between 2.7 and 8%. Changes in olivine CPO symmetry result in minor variations in the seismic anisotropy patterns, mainly in the apparent isotropy directions for shear wave splitting. Seismic properties averaged over 20 km-thick depth sections are, therefore, very homogeneous. Based on these data, we predict the anisotropy that would be measured by SKS, Rayleigh (SV) and Love (SH) waves for five endmember orientations of the foliation and lineation. Comparison to seismic anisotropy data from the Kaapvaal shows that the coherent fast directions, but low delay times imaged by SKS studies, and the low azimuthal anisotropy with with the horizontally polarized S waves (SH) faster than the vertically polarized S wave (SV) measured using surface waves are best explained by homogeneously dipping (45°) foliations and lineations in the cratonic mantle lithosphere. Laterally or vertically varying foliation and lineation orientations with a dominantly NW-SE trend might also explain the low measured anisotropies, but this model should also result in backazimuthal variability of the SKS splitting data, not reported in the seismological data. The strong compositional heterogeneity of the Kaapvaal peridotite xenoliths results in up to 3% variation in density and in up to 2.3% variation of Vp, Vs, and Vp / Vs ratio. Fe depletion by melt extraction increases Vp and Vs, but decreases the Vp / Vs ratio and density. Orthopyroxene enrichment due to metasomatism decreases the density and Vp, strongly reducing the Vp / Vs ratio. Garnet enrichment, which was also attributed to metasomatism, increases the density, and in a lesser extent Vp and the Vp / Vs ratio. Comparison of density and seismic velocity profiles calculated using the xenoliths' compositions and equilibration conditions to seismological data in the Kaapvaal highlights that (i) the thickness of the craton is underestimated in some seismic studies and reaches at least 180 km, (ii) the deep sheared peridotites represent very local modifications caused and oversampled by kimberlites, and (iii) seismological models probably underestimate the compositional heterogeneity in the Kaapvaal mantle root, which occurs at a scale much smaller than the one that may be sampled seismologically.
ISSN: 1869-9510

hal-01053516
https://hal.archives-ouvertes.fr/hal-01053516
DOI : 10.5194/se-5-45-2014

Éditeur(s) : HAL CCSD Elsevier
Résumé : International audience
Seismic tomography and high surface heat flow models indicate an anomalously thin and hot lithospheric mantle beneath the Canadian Cordillera. To understand the thermal and deformational history of the lithospheric mantle under these conditions, we performed a study of the microstructures, crystal preferred orientations (CPO), and seismic anisotropy of mantle xenoliths collected in the Summit Lake volcano in the southern Canadian Cordillera. The xenoliths vary in composition between spinel lherzolites, cpx-rich lherzolites, and wehrlites. All samples are coarse-grained and show clear evidence for annealing, but deformation microstructures are well-preserved in the spinel lherzolites. Olivine CPO is characterized by a strong point-maximum of [100] and by a girdle distribution of [010[ and [001] axes normal to it; CPO strength is variable, spinel-lherzolites have the strongest CPO. Some samples show a weak orthopyroxene CPO consistent with the olivine one. However most pyroxenes have random CPO, interstitial shapes and undeformed appearance, which suggest a secondary origin by reactive melt percolation under static conditions, leading to crystallization of cpx + sp +/- opx at the expenses of olivine, followed by crystallization of cpx + sp +/- ol at the expenses of opx to produce the wehrlites. The studied xenoliths have typical upper mantle seismic properties patterns with fastest propagation of P-waves and polarization of S-waves parallel to the olivine [100] maxima. Maximum P- and S-waves anisotropy for lherzolites are 8 and 5% respectively. Dispersion of the olivine CPO due to neocrystallization of olivine results in weaker anisotropies (6.5 and 3.5%) in wehrlites. Seismic velocities calculated using a geotherm consistent with the equilibrium temperatures of the Summit Lake xenoliths are 3-4% lower than those expected in a normal, 100 km-thick lithosphere, consistent with seismic tomography models for the region. This high geothermal gradient may be partially explained by considering the anisotropy of thermal conductivity of olivine and subhorizontal foliations in the lithospheric mantle, which result in a decrease by up to 15% of the thermal conductivity in the vertical direction.
ISSN: 0040-1951

hal-00618332
https://hal.archives-ouvertes.fr/hal-00618332
DOI : 10.1016/j.tecto.2011.04.014

Éditeur(s) : HAL CCSD AGU and the Geochemical Society
Résumé : International audience
Peridotite xenoliths brought up to the surface by the volcanism of the Kerguelen Islands represent a mantle that has been affected by a high degree of partial melting followed by intense melt percolation above the Kerguelen plume. These xenoliths are therefore particularly suitable to investigate effects of melt-rock interaction on crystallographic fabrics (lattice-preferred orientation (LPO)) of peridotite minerals and on the LPO-induced seismic properties of peridotites above a mantle plume. We have studied a suite of 16 ultramafic samples representative of different degrees of partial melting and magma-rock interaction among which the protogranular harzburgites are the least metasomatised xenoliths and dunites are the ultimate stage of metasomatism. Olivine LPO is characterized by high concentration of [010] axes perpendicular to the foliation and [100] axes close to the lineation or distributed in the foliation plane in harzburgites, whereas the high concentration of [100] axes is parallel to the lineation and [010] axes is perpendicular to the assumed foliation in dunites. Olivine LPO in harzburgites is interpreted as being due to a deformation regime in axial compression or transpression. The fabric strength of olivine decreases progressively from protogranular to poikilitic harzburgites and finally to dunites, for which it remains nevertheless significant (J index 3.8). Seismic properties calculated from LPO of minerals indicate that metasomatism at higher melt/rock ratio lowers the P wave velocities. The most significant difference between harzburgites and dunites corresponds to the distribution of S wave anisotropy. Harzburgites display the maximum of anisotropy within the foliation plane and the minimum of anisotropy perpendicular to the foliation plane, whereas the lowest anisotropy is parallel to the lineation for dunites. These modifications of seismic properties as a result of metasomatic processes may induce seismic heterogeneities in the mantle above the Kerguelen plume. In addition, assuming a lithospheric mantle primarily harzburgitic and structured with a horizontal foliation, the seismic properties calculated for the Kerguelen xenoliths reconcile the rather high anisotropy evidenced by the horizontally propagating surface waves with the apparent isotropy revealed by the absence of splitting of vertically propagating teleseismic SKS waves recorded by the GEOSCOPE Kerguelen station.
ISSN: 1525-2027

hal-00366222
https://hal.archives-ouvertes.fr/hal-00366222
https://hal.archives-ouvertes.fr/hal-00366222/document
https://hal.archives-ouvertes.fr/hal-00366222/file/bascou_Kerguelen_2007GC001879_hal.pdf
DOI : 10.1029/2007GC001879

Éditeur(s) : Université Paul Sabatier, Toulouse III
Résumé : The marine phytoplankton in the ocean represents only less than 1% of global biomass. Phytoplankton performs half of all photosynthesis. This autotrophic biomass in ocean is then an essential element in the climate regulation through processes as carbon dioxide absorption during the photosynthesis. Therefore, we need to estimate precisely this biomass as well as the processes which affect it. Using remotely sensed data (altimetry and ocean colour) and a coupled physical/biogeochemical model (MERCATOR-OPA/NPZDDON), Rossby waves and their influence on phytoplankton biomass are specifically studied in the North Atlantic Ocean. Their features and their influences on surface chlorophyll concentrations were analysed. Through the different mechanisms identified, we estimated that these waves can induce local increases from 60% to 150% of the estimated primary production.
Même si l'océan ne représente que moins de 1% de la biomasse liée aux plantes sur la terre, il est responsable de près de la moitié de la photosynthèse nette de la biosphère. Cette biomasse est par conséquent un élément essentiel dans la régulation du climat à travers par exemple le cycle océanique du carbone. Il est donc nécessaire d'estimer correctement cette biomasse ainsi que les processus qui l'affectent. A l'aide de données satellites altimétriques et couleur de l'eau et d'un modèle couplé physique/biogéochimie (MERCATOR-OPA / NPZDDON), les ondes de Rossby et leur influence sur cette biomasse sont spécifiquement étudiées dans l'océan Atlantique Nord. Leurs propriétés et leur influence sur les concentrations en chlorophylle de surface sont analysées. Au travers des mécanismes mis en jeu identifiés, nous estimons que ces ondes peuvent entraîner une augmentation locale comprise entre 60% et 150% de la production primaire estimée.

Droits : info:eu-repo/semantics/openAccess
http://archimer.ifremer.fr/doc/2005/these-2454.pdf
http://archimer.ifremer.fr/doc/00000/2454/

Éditeur(s) : HAL CCSD Elsevier Masson
Résumé : While oceanic circulation in the Gulf of Lion (GoL) has often been studied in calm weather or with northerly winds (Tramontane or Mistral) through observations and numerical circulation models, few studies have focused on southeasterly storm events. Yet, correct representation of the circulation during storms is crucial if the suspension of sediments is to be correctly modelled throughout the Gulf. The purpose of this paper is to describe the hydrodynamics in the Gulf of Aigues-Mortes (NW of the GoL) during the storm of 18 February 2007 by using a set of data from an ADCP station placed at a depth of 65 m on the sea bed off the coast at Sète, supplemented by the ocean circulation model SYMPHONIE. This storm was characterized by a moderate south-easterly wind (15 m . s−1) and waves of up to 5 m of significant height at its apex. At the ADCP, strong currents of up to 0.8 m . s−1 near the surface and 0.5 m . s−1 near the bottom were recorded, parallel to the coast, flowing towards the south-west. The simulated currents were widely underestimated, even taking the effect of waves into account in the model. It was suspected that the representation of the wind in the atmospheric model was an underestimation. A new simulation was therefore run with an arbitrarily chosen stronger wind and its results were in much better agreement with the measurements. A simplified theoretical analysis successfully isolated the wind-induced processes, responsible for the strong currents measured during the apex and the strong vertical shear that occurred at the beginning of the storm. These processes were: 1/ the barotropic geostrophic current induced by a wind parallel to the coast and 2/ the Ekman spiral. The duration of the storm (about 36 h at the apex) explains the continuous increase of the current as predicted by the theory. The frictionally induced Ekman transport explains the current shear in the surface layer in the rising stage of the storm, and the addition of high waves and strong wind at the apex is more in favour of strong vertical mixing in the surface layer.
ISSN: 1631-0713

hal-00908200
https://hal.archives-ouvertes.fr/hal-00908200
DOI : 10.1016/j.crte.2013.07.001

Éditeur(s) : HAL CCSD Wiley
Résumé : International audience
Climatic changes result in an increased in mean temperature and in a higher incidence of extreme weather events such as heat and cold waves. For ectotherms, such as insect parasitoids, the ability to remain active under extreme climatic conditions is a significant key to fitness. The body size of individuals, and in particular their surface to volume ratio, may play a role in their resistance to thermal conditions. The thermal tolerances are investigated of two closely-related sympatric parasitoid species [Aphidius avenae Haliday and Aphidius rhopalosiphi De Stefani-Perez (Hymenoptera: Aphidiinae)] that have a similar ecology but differ in body size and phenologies. The critical thermal limits of individuals are assessed in both sexes of each parasitoid species and the influence of surface-volume ratios on their thermal tolerances. Aphidius avenae is less resistant to low temperatures and more resistant to high temperatures than A. rhopalosiphi. The lower surface to volume ratio of A. avenae individuals may help them to remain active in summer when experiencing heat waves. However, body size is not the sole factor that plays a role in differences of thermal tolerance between species and body size may not be an adaptation to extreme temperatures but rather a by-product of developmental regulation. Closely related sympatric species from the same ecological guild can have different thermal tolerances that may allow them to occur within the same habitat. The present study also highlights the importance of clearly defining how to measure critical thermal limits to determine the thermal tolerance of a species.
ISSN: 0307-6962

hal-00589094
https://hal.archives-ouvertes.fr/hal-00589094
DOI : 10.1111/j.1365-3032.2010.00758.x

Éditeur(s) : Pergamon-elsevier Science Ltd
Résumé : The south-west lagoon of New Caledonia is a wide semi-open coral reef lagoon bounded by an intertidal barrier reef and bisected by numerous deep inlets. This paper synthesizes findings from the 2000-2008 French National Program EC2CO-PNEC relative to the circulation and the transport of suspended particles in this lagoon. Numerical model development (hydrodynamic, fine suspended sediment transport, wind-wave, small-scale atmospheric circulation) allowed the determination of circulation patterns in the lagoon and the charting of residence time, the later of which has been recently used in a series of ecological studies. Topical studies based on field measurements permitted the parameterisation of wave set-up induced by the swell breaking on the reef barrier and the validation of a wind-wave model in a fetch-limited environment. The analysis of spatial and temporal variability of suspended matter concentration over short and long time-scales, the measurement of grain size distribution and the density of suspended matter (1.27 kg 1(-1)), and the estimation of erodibility of heterogeneous (sand/mud, terrigenous/biogenic) soft bottoms was also conducted. Aggregates were shown to be more abundant near or around reefs and a possible biological influence on this aggregation is discussed. Optical measurements enabled the quantification of suspended matter either in situ (monochromatic measurements) or remotely (surface spectral measurements and satellite observations) and provided indirect calibration and validation of a suspended sediment transport model. The processes that warrant further investigation in order to improve our knowledge of circulation and suspended sediment transport in the New Caledonia lagoon as well as in other coral reef areas are discussed, as are the relevance and reliability of the numerical models for this endeavour.
Marine Pollution Bulletin (0025-326X) (Pergamon-elsevier Science Ltd), 2010 , Vol. 61 , N. 7-12 , P. 269-296

Droits : 2010 Elsevier Ltd All rights reserved.
http://archimer.ifremer.fr/doc/00014/12526/9401.pdf
DOI:10.1016/j.marpolbul.2010.06.023
http://archimer.ifremer.fr/doc/00014/12526/

Éditeur(s) : HAL CCSD Wiley
Résumé : International audience
This study demonstrates that 20-100 GHz range can be used for spatially-accurate focusing of heating inside the skin achieved by varying frequency and exposure beam size, as well as by enforcing air convection. The latter is also used to reduce overheating of skin surface. Heating at different skin depths depending on these parameters is investigated in detail using the hybrid bio-heat equation. In particular, it is shown that decreasing frequency and/or increasing exposure beam size at forced airflow result in elevation of heating of deeper layers of tissue and decrease of skin surface temperature. Changes of water content within 15%, which exceed those due to aging and presence of tumors, only slightly affect heating. Exposure intensity necessary to reach a target temperature significantly increases in different areas of body with elevated blood flow. Dependence on exposure intensity and hyperthermia treatment duration is also investigated and discussed. Results of this study suggest that the lower part of the millimeter-wave range is an attractive alternative for non-invasive thermal treatment of skin cancer with a high spatial resolution. Bioelectromagnetics. 36:464-475, 2015. © 2015 Wiley Periodicals, Inc
ISSN: 0197-8462

hal-01187403
https://hal-univ-rennes1.archives-ouvertes.fr/hal-01187403
DOI : 10.1002/bem.21929
PUBMED : 26179286

Éditeur(s) : HAL CCSD Elsevier
Résumé : International audience
We compare a global compilation of shear-wave splitting measurements with azimuthal seismic anisotropy parameters inferred from surface-wave tomography. The currently available splitting dataset is taken from a novel comprehensive collection of available publications that is updated interactively online. The comparison between the two types of data is made by calculating predicted splitting parameters from the anisotropic tomography model. Comparing these predicted splitting parameters with the observed ones, we find a considerable correlation between the two datasets at global scale. This result is noteworthy, since such correlation did not seem to exist in previous studies. The spatial resolution associated with the two types of methods is rather different. While surface waves have good vertical resolution and poor lateral resolution of several hundreds of kilometers, SKS splitting measurements have good lateral, but poor vertical resolution. The correlation can be understood in light of recent propositions that anisotropy seen by SKS splitting constrains mostly the upper mantle, and therefore a similar depth region as surface waves. The correlation also confirms the generally good quality of the shear-wave measurements, as well as that of the anisotropic tomography model.
ISSN: 0031-9201

hal-00429396
https://hal.archives-ouvertes.fr/hal-00429396
https://hal.archives-ouvertes.fr/hal-00429396/document
https://hal.archives-ouvertes.fr/hal-00429396/file/Wuestefeld_SKS_SW_correlation_PEPI2009-hal.pdf
DOI : 10.1016/j.pepi.2009.05.006

Éditeur(s) : HAL CCSD Elsevier
Résumé : International audience
Constraining mantle deformation beneath plate boundaries where plates interact with each other, such as beneath active or halted mountain belts, is a particularly important objective of "mantle tectonics" that may bring a depth extent to the Earth's surface observation. Such mantle deformation can be mapped at scale lengths of several tens of kilometers through the analysis of seismological data and particularly by mapping seismic anisotropy from the splitting analysis of vertically-propagating SKS waves that largely reflect the strain-induced crystal preferred orientations of the rock-forming minerals within the upper mantle. In the present study, we analyse data from approximately 50 broadband seismic stations covering the Western Alps and we provide a coherent picture of upper mantle anisotropy beneath the belt. The large-scale anisotropy pattern is characterized by fast split directions that closely follow the trend of the belt. Moreover, the maximum anisotropy magnitude is not located beneath the internal zones of the belt but instead beneath external units. All suggests that the anisotropy is likely dominated by sublithospheric mantle deformation. We propose that the observed anisotropy pattern can be explained by recent or active mantle flow around the Eurasian slab presently plunging beneath the inner parts of the Alps.
ISSN: 0012-821X

hal-00617828
https://hal.archives-ouvertes.fr/hal-00617828
https://hal.archives-ouvertes.fr/hal-00617828/document
https://hal.archives-ouvertes.fr/hal-00617828/file/Barruol_SKS_Alpes_EPSL2011_hal.pdf
DOI : 10.1016/j.epsl.2010.12.040

Éditeur(s) : HAL CCSD Elsevier
Résumé : International audience
The subcontinental lithospheric mantle evolves through time due to tectonic events and processes as static recrystallization and melt percolation. To constrain the extent of these processes in the South Patagonian subcontinental mantle lithosphere we performed a microstructural and Electron Backscattered Diffraction (EBSD) study of a suite of 35 peridotite xenoliths brought to the surface by Plio-Pleistocene alkaline volcanic rocks from Gobernador Gregores and Pali Aike. All samples show a well-developed olivine and pyroxene crystallographic preferred orientation (CPO), consistent with deformation by dislocation creep with dominant activation of [100]{0kl} in olivine. The coarse granular or tabular textures and the low density of intracrystalline deformation features indicate that deformation was followed by annealing under static conditions. The xenoliths also show microstructural evidence of multiple episodes of reactive melt percolation. Neither annealing nor melt percolation erased the olivine CPO, which has [010]-fiber, [100]-fiber and orthorhombic patterns in Pali Aike xenoliths and essentially [010]-fiber and orthorhombic patterns in Gobernador Gregores xenoliths. Seismic properties calculated based on the CPO and modal compositions are, however, rather homogeneous, with fast S-wave polarization and P-wave propagation parallel to the [100] olivine axis. The variation in the olivine CPO solely changes the minimum S-wave birefringence direction, which is normal to the foliation for axial-[010] olivine CPO. Average samples for the two localities, obtained by adding up the individual samples CPO data in a common reference frame, show, however, a 'normal' upper mantle anisotropy with a maximum S-wave birrenfringence of ca. 5% at high angle to the both the maximum [010] and [100] axes concentrations, that is in the foliation, but normal to the lineation, and a minimum birefringence at low angle to the [100] maximum, that is parallel to the lineation.
ISSN: 0040-1951

hal-01053500
https://hal.archives-ouvertes.fr/hal-01053500
DOI : 10.1016/j.tecto.2014.02.017

Éditeur(s) : HAL CCSD Institute of Electrical and Electronics Engineers
Résumé : International audience
An effective solution for increasing the exposure uniformity and efficiency of biological samples in in vitro bioelectromagnetic experiments at 60 GHz is proposed by introducing a novel choke ring antenna (CRA). The CRA is optimized to provide a uniform exposure of samples, whose dimensions are equivalent to those of a standard 35-mm Petri dish, positioned close to the antenna aperture, i.e., 10-20 wavelengths. The antenna prototype is fabricated in metallized foam. The realized exposure efficiency of the sample exceeds 55% (if estimated for an exposure uniformity better than - 0.5 dB). To validate the numerical results, the field intensity profiles on the surface of a high-water-content phantom have been experimentally obtained using a high-resolution infrared camera. Compared to the standard open-ended waveguide and horn antennas, typically used for millimeter-wave dosimetry, a twofold advantage of the proposed CRA is demonstrated, namely, the improvement of the exposure efficiency by a factor of 1.5 to 2 with a simultaneous reduction of the exposure distance by a factor of 8-2, respectively, depending on the type of the reference antenna. These advantages make the proposed CRA an excellent candidate for 60-GHz short-range exposure systems for in vitro bioelectromagnetic studies.
ISSN: 0018-9480

hal-00874349
https://hal.archives-ouvertes.fr/hal-00874349
DOI : 10.1109/TMTT.2013.2255619

Éditeur(s) : HAL CCSD Oxford University Press (OUP)
Résumé : International audience
The Ryukyu subduction is known to generate very few earthquakes in its central segment contrarily to its two extremities. We focus in this study on the southernmost part of the Ryukyu subduction zone offshore east Taiwan. Our first task was to build a homogeneous earthquake catalogue for the period 1900–2007. The new catalogue provides homogenized M′W magnitudes and shows that several M′W≥ 7.0 earthquakes occurred offshore Hualien and Suao cities. We then focused on the 1920 June 5 earthquake (reported surface wave magnitude 8.1) previously located beneath the accretionary prism. The revised moment magnitude has been estimated in our catalogue at 7.7 ± 0.2. It is the biggest earthquake ever recorded in the Taiwan area but the fault that has produced this earthquake has not yet been identified with confidence. We relocated this event using regional phases (seismological bulletins archived at the Central Weather Bureau of Taiwan) about 50 km NNE and shallower of its former location, that is, within the Ryukyu Arc basement. According to earthquake bulletin information, revised magnitude, new hypocentral determination and known regional faults, we propose four potential active faults as candidates for the slip associated to this event: (1) the interplate seismogenic zone (ISZ), (2) an out-of-sequence thrust cutting through the forearc and branching on the ISZ, (3) a NS strike-slip fault cutting through the Ryukyu arc and (4) a N–S, westward dipping thrust fault, affecting the Philippine Sea Plate east of the Luzon Arc. The best compromise is to consider a rupture along the ISZ with a shallow nucleation possibly along a splay-fault followed by a downward and lateral propagation of the rupture that would explain the lack of significant seafloor motion and subsequent tsunami. We also estimate the maximum seismic coupling of the ISZ in the region east of Taiwan to about 0.4. In parallel, the evidences of aseismic slip occurring along the ISZ allow us to conclude that this region should only be affected by M < 8 earthquakes.
ISSN: 0956-540X

hal-00545459
https://hal.archives-ouvertes.fr/hal-00545459
https://hal.archives-ouvertes.fr/hal-00545459/document
https://hal.archives-ouvertes.fr/hal-00545459/file/theunisseGJI2010.pdf
DOI : 10.1111/j.1365-246X.2010.04813.x

Éditeur(s) : HAL CCSD AGU and the Geochemical Society
Résumé : International audience
How reliable are shear wave splitting measurements as a means of determining mantle flow direction? This remains a topic of debate, especially in the context of subduction. The answer hinges on whether our current understanding of mineral physics provides enough to accurately translate between seismic observations and mantle deformation. Here, we present an integrated model to simulate strain-history-dependent texture development and estimate resulting shear wave splitting in subduction environments. We do this for a mantle flow model that, in its geometry, approximates the double-sided Molucca Sea subduction system in Eastern Indonesia. We test a single-sided and a double-sided subduction case. Results are compared to recent splitting measurements of this region by Di Leo et al. (2012a). The setting lends itself as a case study, because it is fairly young and, therefore, early textures from the slab's descent from the near surface to the bottom of the mantle transition zone--which we simulate in our models--have not yet been overprinted by subsequent continuous steady state flow. Second, it allows us to test the significance of the double-sided geometry, i.e., the need for a rear barrier to achieve trench-parallel subslab mantle flow. We demonstrate that although a barrier amplifies trench-parallel subslab anisotropy due to mantle flow, it is not necessary to produce trench-parallel fast directions per se. In a simple model of A-type olivine lattice-preferred orientation and one-sided subduction, trench-parallel fast directions are produced by a combination of simple shear and extension through compression and pure shear in the subslab mantle.
ISSN: 1525-2027

hal-01053506
https://hal.archives-ouvertes.fr/hal-01053506
DOI : 10.1002/2013GC005032

Éditeur(s) : HAL CCSD
Résumé : This study provides additional constraints on the relations between deformation, hydration and percolation of fluids and melts in the subcontinental lithospheric mantle beneath a craton and a rift, as well as their implication on its geodynamical behaviour. I have analysed the microstructures, the CPOs, and the hydrogen content of mantle xenoliths from the Kaapvaal craton, and two sets of xenoliths from different localities along the East African Rift (North Tanzanian Divergence and SE Ethiopia).The coarse-granular microstructures and the well-defined CPOs in Kaapvaal peridotites suggest a deformation followed by a long quiescence time. Orthorhombic olivine CPOs predominates, but axial-[100] and axial-[010] are also measured. Cratonic peridotites record multiple metasomatic episodes, leading to a significant compositional heterogeneity, which cannot be imaged by seismic studies. Olivine hydrogen contents are variable, but tend to increase until 150 km depth, reaching up to 50 ppm wt. H2O. The deeper samples are almost dry. Piston-cylinder experiments on hydrogen diffusion between a volatile-rich kimberlitic melt and forsterite suggest that the presence of CO2 in the system could significantly decrease water fugacity and thus forsterite hydration. These experimental results indicate that the hydrogen contents measured in olivine were acquired during a metasomatic event rather than during xenolith extraction by kimberlites. However, this metasomatism was not followed by remobilization of the cratonic root.In the North Tanzanian Divergence, localities within the rift axis and the volcanic transverse belt (Lashaine and Olmani) show significant differences in microstructures and olivine CPO patterns. In Lashaine, coarse-granular microstructures and orthorhombic to axial-[100] CPO patterns in olivine can be explained by transpressional deformation during the formation of the Mozambique belt, or by the occurrence of a remnant of a cratonic domain embedded within the Mozambique belt. Within the rift axis, porphyroclastic to mylonitic microstructures suggest a recent rift-related deformation accompanied by syn-kinematic melt-rock reactions, and followed by variable annealing. The strong heterogeneity in microstructures and olivine CPO suggests that this deformation was acquired during multiple tectonic events probably linked to episodic magma percolation, separated by quiescence episodes. The axial-[100] patterns in olivine and the oblique fast directions reported by SKS studies are coherent with transtensional deformation within the lithospheric mantle beneath the rift.The peridotites from SE Ethiopia are less recrystallized than the rift-axis Tanzanian peridotites, displaying coarse-porphyroclastic microstructures. Microstructures and orthorhombic CPOs in olivine suggest syn- to post-metasomatic deformation. S-waves polarization anisotropies calculated for these samples cannot explain alone the delay times reported by SKS studies in this part of the East-African Rift.
Les travaux réalisés durant cette thèse apportent de nouvelles contraintes sur les relations entre déformation, hydratation et percolation de fluides et/ou de magmas dans le manteau subcontinental sous un craton et sous un rift, et leurs implications sur son comportement rhéologique. Il repose sur l'analyse des microstructures, des OPRs et des teneurs en hydrogène de xénolites mantelliques du craton du Kaapvaal, et sur deux séries de xénolites provenant de différentes localités le long du rift Est-Africain (Divergence Nord Tanzanienne et SE de l'Ethiopie). Les microstructures granulaires à gros grains et les OPRs bien définies des péridotites du craton du Kaapvaal sont cohérentes avec un épisode de déformation suivi d'une longue période de quiescence. Les OPRs de l'olivine sont majoritairement à symétrie orthorhombique, mais des symétries axiale-[100] et axiale-[010] sont aussi mesurées. Les péridotites cratoniques enregistrent de multiples épisodes métasomatiques, ayant entraîné une hétérogénéité de compositions à petite échelle ne pouvant être détectée par les études sismiques. Les teneurs en hydrogène mesurées dans l'olivine sont variables, mais ont tendance à augmenter jusqu'à 150 km de profondeur, atteignant alors jusqu'à 50 ppm wt. H2O. En dessous de cette profondeur, les échantillons montrent des teneurs en hydrogène très faibles. Les expériences réalisées en piston-cylindre sur la diffusion de l'hydrogène issue d'un liquide kimberlitique vers de la forstérite suggèrent que la fugacité en eau pourrait fortement être diminuée par la présence de CO2, empêchant l'hydratation de l'olivine durant extraction des xénolites par les kimberlites. Ces résultats expérimentaux suggèrent que les teneurs en hydrogène dans l'olivine des péridotites du craton du Kaapvaal ont été acquises durant un épisode métasomatique en profondeur et non pendant leur extraction par les kimberlites. Ces teneurs n'ont toutefois pas à ce jour entraîné de remobilisation de la racine cratonique. Enfin, le calcul des propriétés sismiques des péridotites cratoniques révèle que les anisotropies générées par les OPRs de ces échantillons sont suffisantes pour expliquer les anisotropies mesurées par les ondes SKS et les ondes de surface.Les xénolites de la Divergence Nord-Tanzanienne, montrent des variations significatives de microstructures et d'OPR de l'olivine entre les péridotites des localités dans l'axe du rift et celles de la chaîne volcanique transverse (Lashaine et Olmani). A Lashaine, les microstructures granulaires à gros grains et les OPRs de type orthorhombique et axial-[010] peuvent être expliquée par une déformation en transpression liée à la formation de la chaîne Mozambique ou par la présence d'une relique d'un domaine cratonique à l'intérieur de la chaîne Mozambique. Dans l'axe du rift, les microstructures porphyroclastiques à mylonitiques suggèrent une déformation plus récente, accompagnée de réactions magma-roche sous des conditions proches du solidus, suivie d'un recuit variable. L'hétérogénéité des microstructures enregistrées par les échantillons du rift suggère de multiples épisodes de déformation localisée, probablement liés à l'injection percolation épisodique de magmas, espacés de périodes d'accalmie. Les OPRs de l'olivine de type axial-[100] et l'orientation des directions de polarisation des ondes SKS suggère que le rift s'est formé en régime de transtension Les péridotites du Sud-Est de l’Éthiopie présentent des microstructures porphyroclastiques à gros grains moins recristallisées qu'en Tanzanie. Les microstructures et les OPRs principalement de type orthorhombique suggèrent une déformation syn- à post-métasomatisme. Les anisotropies de polarisation des ondes S calculées pour ces échantillons sont insuffisantes pour expliquer à elles seules les déphasages des ondes SKS dans cette partie du rift.
https://tel.archives-ouvertes.fr/tel-01684759

Droits : info:eu-repo/semantics/OpenAccess
NNT : 2014MON20139
tel-01684759
https://tel.archives-ouvertes.fr/tel-01684759
https://tel.archives-ouvertes.fr/tel-01684759/document
https://tel.archives-ouvertes.fr/tel-01684759/file/43443_BAPTISTE_2014_archivage_cor.pdf

Éditeur(s) : HAL CCSD
Résumé : Report
The Canadian coastline is the longest of any country in the world, and is at risk from tsunamis generated in three oceans. The current state of knowledge precludes a complete probabilistic tsunami hazard assessment, which would require quantification of a wide range of possible scenarios for each tsunami source, coupled with modelling that incorporates fine-resolution bathymetry and onland topography to adequately assess potential runup at the coast. This preliminary assessment presents a first attempt to quantify the tsunami hazard on the Canadian Pacific, Atlantic and Arctic coastlines from local and far-field, earthquake and large landslide sources. For each source considered, we calculate the probability that tsunami runup at the coast will exceed 1.5 m (threshold for potential damage) and 3 m (significant damage potential), in a 50-year period. For each coastal region, we then combine the relative hazard from each source to calculate the overall probability that the coastline in question will experience tsunami runup exceeding 1.5 m (and 3 m) within a 50-year period, from any geological source. We also consider the maximum runup levels expected to occur within time periods of 100, 500, 1000, and 2500 years.
https://hal.archives-ouvertes.fr/hal-00856677

hal-00856677
https://hal.archives-ouvertes.fr/hal-00856677
DOI : 10.4095/292067

Éditeur(s) : HAL CCSD Elsevier
Résumé : International audience
The interpretation of high-resolution 2D marine seismic profiles together with the analysis of sea-bottom cores allowed a stratigraphic and structural framework of the Provence continental shelf to be proposed. The integration of onshore and offshore stratigraphy, structure and geomorphology provided new insights into Messinian paleotopography and paleohydrography. A geological map of the offshore Provence continental shelf, isobath map of the base Plio-Quaternary surface are presented for the first time in this area. The base Plio-Quaternary surface is a polyphased unconformity that is composed of deep canyons developed by fluvial erosion during the Messinian event, and wave-cut surfaces formed during post-Messinian transgressions. The study evidenced a deep, E-W-trending canyon (Bandol canyon) connected to the head of the Cassidaigne canyon, and filled with up to 600 m-thick Plio-Quaternary deposits. The development of canyons on the Provence margin during the Messinian event was dominantly controlled by the lithology and structure of pre-Messinian formations. A map of the Messinian paleo-drainage network is proposed to explain the presence of deep canyons in the Eastern area and the lack of incision in the Western area. An underground karst drainage scheme is proposed, linked with the current submarine Port-Miou spring.
ISSN: 0264-8172

insu-01009592
https://hal-insu.archives-ouvertes.fr/insu-01009592
https://hal-insu.archives-ouvertes.fr/insu-01009592/document
https://hal-insu.archives-ouvertes.fr/insu-01009592/file/Mar-Pet-Tassy.pdf
DOI : 10.1016/j.marpetgeo.2014.05.001

Éditeur(s) : Gauthier-Villars
Résumé : The wind, temperature and current data collected during the joined French-USA FOCAL/SEQUAL programme carried in the equatorial Atlantic band in 1983 and 1984, allowed for the first time a simultaneous study of both the equatorial and coastal (5-degrees-N) upwellings along 4-degrees-W. At the equator, the decreases of both the surface temperature and the depth of the thermocline in boreal summer are correlated with the zonal component of the local windstress in the period band 1-2 months. This corresponds to the time required by the thermocline to lift up at 0-4-degrees-W in the presence of an easterly wind. The local wind cannot however explain the entire vertical variability of the thermal structure at 0-4-degrees-W. The correlation function shows in addition that the temperature there is highly correlated to the wind recorded at St Peter and St Paul Rocks (SPP) in the period band 0-2 months. This time scale is the one required by the Kelvin and Rossby waves to propagate along the equator through equatorial wave guide dynamics. The influence of these waves seems particularly important when considering the upward displacements of the thermocline both in February-March when the winds abruptly relax and in November-December when the wind at 0-4-degrees-W is southwesterly with no secondary negative maximum in the wind record at 1-degrees-N-29-degrees-W (SPP), contrary to the climatological wind field. The computation of the different terms of the heat equation applied to an homogeneous surface layer shows that the cooling is entirely due to vertical advection and eddy diffusion. The cooling would be more effective without the atmospheric heat flux and the horizontal advection of heat transported both by the South Equatorial Current at the surface and the southward current at the bottom of the mixed layer. At the coast, the amplitude and duration of the upwellings are not constant all along the coast: in boreal summer, they are maximum east of the two capes (Palmas and Three Pointes) while in winter the strength of the cooling is maximum in the east of Cape Palmas and then decreases eastward. The meridional slope of the thermocline off the coast concerns a distance which is much larger than the internal Rossby radius of deformation and is coherent with the latitudinal extension and intensity of the Guinea current both in summer and winter. In addition to this geostrophic adjustment, the upward tilt of the thermocline in summer is enhanced by the intensification of the component of the wind parallel to the coast. The application of a simple linear model forced with an eastward wind at that time explains the surface maximum cooling at the coast and the current distribution in both vertical and meridional directions. The two maxima of the zonal component of the Guinea current, in summer (main one) and in winter are in phase with the intensity of the vertical component of the windcurl. The application of the Sverdrup equations shows that the current speeds computed, for both seasons, are of the same order of magnitude as the speeds observed.
Oceanologica Acta (0399-1784) (Gauthier-Villars), 1991 , Vol. 14 , N. 3 , P. 223-240

Droits : info:eu-repo/semantics/openAccess
http://archimer.ifremer.fr/doc/00101/21252/18865.pdf
http://archimer.ifremer.fr/doc/00101/21252/