Éditeur(s) : HAL CCSD
Résumé : International audience
This article describes a previously unreported mutation at position 210 (C210T) of the mitochondrial large subunit ribosomal RNA (mtLSUrRNA) gene of Pneumocystis jirovecii, which led to a false-negative result of a real-time polymerase chain reaction (PCR) assay. Since the aforementioned real-time PCR assay is widely used in France, a French multicenter study was conducted to estimate the mutation frequency and its potential impact on the routine diagnosis of Pneumocystis pneumonia (PCP). Through analysis of data obtained from eight centers, the mutation frequency was estimated at 0.28%. This low frequency should not call into question the routine use of this PCR assay. Nonetheless, the occurrence of the false-negative PCR result provides arguments for maintaining microscopic techniques combined to PCR assays to achieve PCP diagnosis.
Medical Mycology

hal-01477200
https://hal-univ-rennes1.archives-ouvertes.fr/hal-01477200
DOI : 10.1093/mmy/myw051
PUBMED : 27489302

Éditeur(s) : HAL CCSD Cambridge University Press (CUP)
Résumé : International audience
Leishmania parasites can escape the immune response by invading cell types lacking leishmanicidal mechanisms. Silent persistence of Leishmania parasites in the host organism is responsible for asymptomatic carriage and relapses after cured leishmaniasis. Here, we studied the interaction between Hepatic Stellate Cells (HSC) and Leishmania. An original model of human HSC in primary culture infected with L. donovani was developed. The presence of intracellular parasites was studied and quantified using optical and confocal microscopy. HSC characteristics were studied using microscopy, methylene blue assay, long-term cultures and qPCR. We showed for the first time that human HSC are permissive to L. donovani infection, with no modification of HSC survival, growth rate and proinflammatory and fibrogenic characteristics. Intracellular parasites did not replicate but HSC had no effect on their survival. Indeed, after a 40-day culture, infected HSC cultures transferred on NNN medium yielded new promastigotes that were able to proliferate and efficiently infect new cells. HSC are permissive to L. donovani, with neither parasite killing nor apparent cell damage. Thus, HSC could act as potent sanctuary cells for Leishmania in the liver, which could partially explain parasite reactivation after an asymptomatic carriage or a cured visceral leishmaniasis.
ISSN: 0031-1820

inserm-00825803
http://www.hal.inserm.fr/inserm-00825803
DOI : 10.1017/S0031182012001965
PUBMED : 23253952

Éditeur(s) : HAL CCSD Springer Verlag
Résumé : International audience
The actin cytoskeleton is a dynamic structure that provides an interactive platform for organelles and cellular components. It also serves as track for membranes and vesicles that move via myosin. The actin cytoskeleton of Symbiodinium is a well-organized reticular structure suggestive of multiple membrane interactions, very likely including those of the chloroplast. The Symbiodinium chloroplast membrane network is, in turn, a highly organized structure, suggestive of being under the control of an organizing network. We visualized the chloroplast membranes of cultured Symbiodinium sp. under various light conditions and observed changes dependent on illumination intensity. Since we suspected interaction between these two organelles, and we knew that the Symbiodinium actin cytoskeleton collapses upon treatment with either latrunculin B, an actin microfilament-disrupting agent, or butanedione monoxime, a myosin function inhibitor, we tested the Symbiodinium sp. oxygen evolution in their presence. Upon latrunculin B addition, the oxygen production decreased compared to non-treated cells; however, this was not observed after a 24 h latrunculin treatment. On the contrary, butanedione monoxime treatment caused a non-recoverable dysfunction of the chloroplast causing a severe loss in oxygen production even after long-term exposure. Using electron microscopy, we observed an alteration of the Symbiodinium sp. chloroplast distribution after latrunculin B treatment, with respect to untreated cells. Furthermore, a thorough disorganization of the chloroplast grana was observed after butanedione monoxime treatment. These data suggest that an actomyosin system would be important for chloroplast organization and distribution, and critical for normal photosynthetic function of Symbiodinium sp.
ISSN: 0166-8595

hal-01546164
https://hal.archives-ouvertes.fr/hal-01546164
DOI : 10.1007/s11120-015-0142-9

Éditeur(s) : HAL CCSD Springer Verlag
Résumé : International audience
Glioblastoma (GB) is the most frequent and aggressive type of primary brain tumor. Recurrences are mostly located at the margin of the resection cavity in the peritumoral brain zone (PBZ). Although it is widely believed that infiltrative tumor cells in this zone are responsible for GB recurrence, few studies have examined this zone. In this study, we analyzed PBZ left after surgery with a variety of techniques including radiology, histopathology, flow cytometry, genomic, transcriptomic, proteomic, and primary cell cultures. The resulting PBZ profiles were compared with those of the GB tumor zone and normal brain samples to identify characteristics specific to the PBZ. We found that tumor cell infiltration detected by standard histological analysis was present in almost one third of PBZ taken from an area that was considered normal both on standard MRI and by the neurosurgeon under an operating microscope. The panel of techniques used in this study show that the PBZ, similar to the tumor zone itself, is characterized by substantial inter-patient heterogeneity, which makes it difficult to identify representative markers. Nevertheless, we identified specific alterations in the PBZ such as the presence of selected tumor clones and stromal cells with tumorigenic and angiogenic properties. The study of GB-PBZ is a growing field of interest and this region needs to be characterized further. This will facilitate the development of new, targeted therapies for patients with GB and the development of approaches to refine the per-operative evaluation of the PBZ to optimize the surgical resection of the tumor.
ISSN: 0167-594X

hal-01117083
https://hal-univ-rennes1.archives-ouvertes.fr/hal-01117083
https://hal-univ-rennes1.archives-ouvertes.fr/hal-01117083/document
https://hal-univ-rennes1.archives-ouvertes.fr/hal-01117083/file/Characterizing%20the%20Peritumoral%20Brain%20Zone_accepted.pdf
DOI : 10.1007/s11060-014-1695-8

Éditeur(s) : HAL CCSD Elsevier
Résumé : We have studied the phase transformation of forsterite to wadsleyite under shear stress at the Earth's transition zone pressure and temperature conditions. Two-step experiments were performed using a multi-anvil press. First, we hot pressed iron-free forsterite at 6 or 11 GPa and 1100 degrees C. Then we deformed a slab of this starting material using a direct simple shear assembly at 16 GPa and 1400 degrees C for 1, 15, 35, 40, or 60 min. Both the starting material and the deformed samples were characterized using optical and scanning electron microscopy including measurements of crystal preferred orientations (CPO) by electron back scattered diffraction (EBSD), transmission electron microscopy (TEM), and Fourier transform infrared spectroscopy (FTIR). The phase transformation occurs very rapidly, in less than 1 min, and metastable forsterite relics are not observed after deformation. The grain size of wadsleyite is slightly smaller than the forsterite starting material. The water contents obtained from FTIR analyses in forsterite and wadsleyite are 65-124 wt ppm H2O and 114-736 wt ppm H2O, respectively, which are well below water solubility at similar conditions in the presence of free water. Wadsleyite aggregates display weak CPO patterns with [1 0 0] axes concentrated at low angle to the shear direction, [0 1 0] axes perpendicular to the shear plane and nearly random [0 0 1] axes. Only a few dislocations were observed in wadsleyite with TEM. This observation is consistent with the assumption that most dislocations formed during the initial high-stress stages of these stress-relaxation experiments, were consumed in the phase transformation, probably enhancing the transformation rate. CPO patterns vary as a function of the water content: with increasing water content the density of [1 0 0] axes parallel to the shear direction decreases, and the density of [0 0 1] axes increases. Viscoplastic self-consistent modeling of CPO evolution using previously reported glide systems for wadsleyite, i.e., [1 0 0]{0 k 1} and 1/2 (1 1 1){1 0 1}, cannot reproduce the measured CPO, unless the [0 0 1](0 1 0) system, for which dislocations have not been observed by TEM, is also activated. In addition, wadsleyite grain growth suggests the participation of diffusion-assisted processes in deformation. Calculated anisotropies for P and S-waves using measured CPO are always below 1%. This very low anisotropy is due to both the low finite strain achieved in the experiments, which leads to weak wadsleyite CPO, and to the diluting effect of added majorite. The present experiments emphasize the importance of stress, grain size evolution and water content in the forsterite to wadsleyite phase transformation and subsequent deformation in the transition zone.
ISSN: 0031-9201

hal-00617430
https://hal.archives-ouvertes.fr/hal-00617430
DOI : 10.1016/j.pepi.2010.11.001

Éditeur(s) : HAL CCSD Optical Society Optical Society - SOA Publishing
Résumé : International audience
We show that the canonical single frequency sarcomeric SHG intensity pattern (SHG-IP) of control muscles is converted to double frequency sarcomeric SHG-IP in preserved mdx mouse gastrocnemius muscles in the vicinity of necrotic fibers. These double frequency sarcomeric SHG-IPs are often spatially correlated to double frequency sarcomeric two-photon excitation fluorescence (TPEF) emitted from Z-line and I-bands and to one centered spot SHG angular intensity pattern (SHG-AIP) suggesting that these patterns are signature of myofibrillar misalignement. This latter is confirmed with transmission electron microscopy (TEM). Moreover, a good spatial correlation between SHG signature of myofibrillar misalignment and triad reduction is established. Theoretical simulation of sarcomeric SHG-IP is used to demonstrate the correlation between change of SHG-IP and -AIP and myofibrillar misalignment. The extreme sensitivity of SHG microscopy to reveal the submicrometric organization of A-band thick filaments is highlighted. This report is a first step toward future studies aimed at establishing live SHG signature of myofibrillar misalignment involving excitation contraction defects due to muscle damage and disease.
ISSN: 2156-7085

hal-00952823
https://hal.archives-ouvertes.fr/hal-00952823
https://hal.archives-ouvertes.fr/hal-00952823/document
https://hal.archives-ouvertes.fr/hal-00952823/file/boe-5-3-858_002.pdf
DOI : 10.1364/BOE.5.000858

Éditeur(s) : HAL CCSD Wiley
Résumé : International audience
Digestive tubules of Strombidae are composed by three cell types: digestive cells, vacuolated cells, and crypt cells. The last one is characterized by the presence of intracellular granules identified as spherocrystals. Such structures are known to occur in basophilic cells of gastropod digestive gland, where they are supposed to be involved in the regulation of some minerals and in detoxification. In this study, energy-dispersive X-ray analysis (EDX) and energy filtered transmission electron microscopy (EFTEM) were used to determine the elemental content of spherocrystals in two Strombidae, Strombus gigas and Strombus pugilis. In freshly collected individuals of both species, the following elements were detected: Ca, Fe, Mg, P, and Zn. Aluminum and Mn were also detected in S. gigas. Their presence in spherocrystals indicates that, in Strombidae, spherocrystals are involved in the regulation of minerals and essential trace metals. In order to answer the question ''are spherocrystals involved in nonessential trace metals scavenging?,'' artificial cadmium and lead exposure by both waterborne and dietary pathways was applied to S. pugilis. No evidence of cadmium (Cd(NO3)2) or lead (Pb(NO3)2) provided by food was found in spherocrystals. Cadmium provided in water (Cd(NO3)2 and CdCl2) causes structural modifications of the digestive gland; however, this element was not trapped in spherocrystals. These results suggest that spherocrystals are not involved in detoxification of such nonessential trace metals.
ISSN: 1059-910X

hal-00743762
https://hal.univ-antilles.fr/hal-00743762
DOI : 10.1002/jemt.21073

Éditeur(s) : HAL CCSD wiley
Résumé : International audience
The slow advance of a crack in sodo-silicate glasses was studied at nanometer scale by in-situ atomic force microscopy (AFM) in a well-controlled atmosphere (N2 and H2O). An enhanced diffusion of sodium ions in the stress-gradient field at the sub-micrometric vicinity of the crack tip was revealed through several effects: growth of nodules in height images, changes in the AFM tip–sample energy dissipation as detected in phase images. Ex-situ chemical micro-analyses completed the AFM measurements. The nodules patterns revealed a dewetting phenomenon evidenced by “breath figures”, i.e. analog to the fogging that occurs when a vapour condenses onto a 'cold' surface [D. Beysens et al., Phys. Rev. Let. 57, 1433 (1986)]. These experimental results were explained by a two-step process: i) a fast migration of sodium ions towards the fracture surfaces as proposed by Langford et al. [J. Mat. Res. 6, 1358 (1991)], ii) a slow backwards diffusion of the cations as evidenced in these AFM experiments (typical time: few minutes). Measurements of the diffusion coefficient of that relaxing process were done at room temperature. Our results strengthen the theoretical concept of a near-surface structural relaxation due to the stress-gradient at the vicinity of the crack tip. Raman and SIMS studies revealed that nodules – for samples studied after exposition to common air - are preferentially covered by an organic overlayer of a carboxylate salt with a long aliphatic chain. The catalytic role of sodium ions in that chemical process is suspected.
Fractography of glasses an ceramics V
Rochester, United States

hal-00408605
https://hal.archives-ouvertes.fr/hal-00408605

Éditeur(s) : HAL CCSD
Résumé : Staphylococcus aureus can cause a wide range of infections in humans and warm-blooded animals. The epithelial surface is the principal site of staphylococcal infection. The goal of this work was to investigate the cytopathic effect of S. aureus and its ability to affect host cell cycle. Cultures of human cervix cancer HeLa and mammary gland bovine MAC-T epithelial cells were used to study in vitro the effects of S. aureus strains from human or ruminant origins. Using microscopic examination, we found that S. aureus induced an enlargement of the host cells and we observed that S. aureus slowed down cell proliferation. Similar effect was observed on both human and bovine cells using the human or the ruminant strains. Microscopic analysis of DAPI-stained cells revealed huge decrease of mitotic indexes in infected HeLa and Mac-T cells, synchronized by Double Thymidine Block. Flow cytometry analysis showed that S. aureus induces G2/M phase delay in synchronous HeLa cells. Western blot analysis revealed that the G2/M phase delay was associated with an accumulation of inactive phosphorylated cyclin-dependent kinase Cdk1/cdc2, a key inducer of mitosis entry, and dephosphorylation of core histone H3, an important component of the epigenetic landscape. Altogether, these results suggest that the delay in G2/M phase transition of the infected epithelial cells may be one of the mechanisms employed by S. aureus to survive and to propagate inside the host. Further investigations are ongoing to fully understand the mechanisms associated to this type of S. aureushost cell interactions.
journées des Microbiologistes de l' INRA 2012
L'Isle sur la Sorgue, France

hal-01209365
https://hal.archives-ouvertes.fr/hal-01209365
PRODINRA : 174569

Éditeur(s) : HAL CCSD
Résumé : Staphylococcus aureus can cause a wide range of infections in humans and warm-blooded animals. The epithelial surface is the principal site of staphylococcal infection. The goal of this work was to investigate the cytopathic effect of S. aureus and its ability to affect host cell cycle. Cultures of human cervix cancer HeLa and mammary gland bovine MAC-T epithelial cells were used to study in vitro the effects of S. aureus strains from human or ruminant origins. Using microscopic examination, we found that S. aureus induced an enlargement of the host cells and we observed that S. aureus slowed down cell proliferation. Similar effect was observed on both human and bovine cells using the human or the ruminant strains. Microscopic analysis of DAPI-stained cells revealed huge decrease of mitotic indexes in infected HeLa and Mac-T cells, synchronized by Double Thymidine Block. Flow cytometry analysis showed that S. aureus induces G2/M phase delay in synchronous HeLa cells. Western blot analysis revealed that the G2/M phase delay was associated with an accumulation of inactive phosphorylated cyclin-dependent kinase Cdk1/cdc2, a key inducer of mitosis entry, and dephosphorylation of core histone H3, an important component of the epigenetic landscape. Altogether, these results suggest that the delay in G2/M phase transition of the infected epithelial cells may be one of the mechanisms employed by S. aureus to survive and to propagate inside the host. Further investigations are ongoing to fully understand the mechanisms associated to this type of S. aureus-host cell interactions.
International Symposium on Staphylococci and Staphylococcal Infections -ISSSI-2012
Lyon, France

hal-01209356
https://hal.archives-ouvertes.fr/hal-01209356
PRODINRA : 173265

Éditeur(s) : HAL CCSD SAGE Publications (UK and US)
Résumé : International audience
In this article, we focus on hypomaturation autosomal-recessive-type amelogenesis imperfecta (type IIA2) and describe 2 new causal Matrix metalloproteinase 20 (MMP20) mutations validated in two unrelated families: a missense mutation p.T130I at the expected homozygous state, and a compound heterozygous mutation having the same mutation combined with a nucleotide deletion, leading to a premature stop codon (p.N120fz*2). We characterized the enamel structure of the latter case using scanning electron microscopy analysis and microanalysis (Energy-dispersive X-ray Spectroscopy, EDX) and confirmed the hypomaturation-type amelogenesis imperfecta as identified in the clinical diagnosis. The mineralized content was slightly decreased, with magnesium substituting for calcium in the crystal structure. The anomalies affected enamel with minimal inter-rod enamel present and apatite crystals perpendicular to the enamel prisms, suggesting a possible new role for MMP20 in enamel formation.
ISSN: 0022-0345

hal-01545484
https://hal.archives-ouvertes.fr/hal-01545484
DOI : 10.1177/0022034513488393

Éditeur(s) : Gauthier-Villars
Résumé : Calcareous nannofossil assemblages of the family Noelaerhabdaceae have been studied in sediments of seventeen Quaternary cores raised from various climatic areas of the Atlantic and Pacific oceans. These sections display a regular, hiatus-free sedimentation for the last 500000 years. They have been dated using oxygen-isotopic measurements, variations of calcium carbonate content, variations of total nannofossil abundance, thorium measurements and volcanic ash horizons. Coccoliths of the genus Gephyrocapsa have been placed in three distinct taxonomic groups, based on morphological criteria inferred from light-microscope observations. Their acmes, as well as the acme of Emiliania huxleyi, are considered as stratigraphic markers. These markers characterize most of the last thirteen glacial-interglacial climatic stages, as well as some climatic events of lower magnitude (8.5 and 6.5). Most of these markers have global stratigraphic significance: acme of ''closed'' Gephyrocapsa during stages 13,11 and 9, and during event 8.5; acme of ''small'' Gephyrocapsa during stage 7 and event 6.5; acme of ''open'' Gephyrocapsa at top of event 6.5, and during stages 5 and 3; acme of E. huxleyi during stages 3 and 1. Others are only valid south of the southernmost latitude reached by the North-Atlantic polar front during the Quaternary: acme of ''closed'' Gephyrocapsa during stage 12; acme of ''small'' Gepkyrocapsa in early and middle stage 5; acme of E. huxleyi during stage 2. Others are only valid south of 20-degrees-S: acme of ''small'' Gephyrocapsa at the 10/9 stage boundary; acme of ''open'' Gephyrocapsa during stage 6.
Oceanologica Acta (0399-1784) (Gauthier-Villars), 1993 , Vol. 16 , N. 4 , P. 349-362

Droits : info:eu-repo/semantics/openAccess
http://archimer.ifremer.fr/doc/00099/21061/18687.pdf
http://archimer.ifremer.fr/doc/00099/21061/

Éditeur(s) : Pergamon-elsevier Science Ltd
Résumé : Pearl culture industry represents one of the dominant business sector of French Polynesia. However, it still entirely relies on unpredictable spat collection success. Our aim was to assess the influence of natural plankton concentration fluctuations on maturation and spawning of the black lip pearl oyster Pinctada margaritifera, during a 4 months survey conducted in Ahe atoll lagoon. Plankton concentration was assessed by chlorophyll a extraction and by microscope counts while gonadic index, gonado-visceral dry weights and histology were used to measure pearl oysters reproduction activity. We found that (i) plankton concentration fluctuations were mainly related to wind regime, (ii) gametogenesis rate was mainly related to plankton concentration, (iii) spawning occurred when maximal gonad storage was reached. (iv) plankton concentration was the main spawning synchronizing factor. These results contribute explaining P. margaritifera spat collection variability in French Polynesian atoll lagoon. (C) 2012 Elsevier Ltd. All rights reserved.
Marine Pollution Bulletin (0025-326X) (Pergamon-elsevier Science Ltd), 2012 , Vol. 65 , N. 10-12 , P. 463-470

Droits : 2012 Elsevier Ltd. All rights reserved
http://archimer.ifremer.fr/doc/00101/21201/19697.pdf
DOI:10.1016/j.marpolbul.2012.03.027
http://archimer.ifremer.fr/doc/00101/21201/

Éditeur(s) : HAL CCSD Magnolia Press
Résumé : International audience
Several diatoms in the family Achnanthaceae (Bacillariophyta), mainly from marine environments, have species with strongly apiculate, lemon-shaped valves. Some of them originally described under the genus Cocconeis (i.e., C. trachyderma or C. citronella), while others were first described as Stauroneis species [i.e. Stauroneis apiculata or S.(?) obesa]. Afterwards, Cocconeis citronella has been recombined within Achnanthes by Hustedt. The type material of C. citronella from Albert Mann’s collection, housed in the Smithsonian Institution (US), has been examined with light microscope; some ambiguities are pointed out and new details added to the original description. The intricate history of the latter taxon is redrawn and comparison with allied taxa are tentatively addressed. Cocconeis trachyderma is lectotypified and recombined as Achnanthes trachyderma comb. nov. Stauroneis apiculata and S.(?) obesa are recombined as Achnanthes apiculata comb. nov. and Achnanthes obesa comb. nov. respectively. The examination with light and scanning electron microscope of several marine samples from the Society Archipelago details the unique morphology of Achnanthes trachyderma which, until recently, has been often misidentified as Achnanthes citronella due to certain similarities between both taxa.
ISSN: 1179-3155

hal-01212892
https://hal-univ-perp.archives-ouvertes.fr/hal-01212892
DOI : 10.11646/phytotaxa.227.2.1

Éditeur(s) : HAL CCSD Taylor & Francis: STM Behavioural Science and Public Health Titles Taylor & Francis: STM, Behavioural Science and Public Health Titles
Résumé : We have investigated by electron tomography, in a transmission electronic microscope, the interactions between dislocations in olivine single crystals and polycrystals deformed in axial compression at T < 1000 °C (T < 0.5Tm). Dislocations are mostly of the [0 0 1] type, except in the polycrystal where [1 0 0] and [0 0 1] dislocations have been activated. A few 〈1 0 1〉 junctions have been found and characterized. Many collinear interactions have been identified either involving direct interactions between crossing dislocations of opposite Burgers vectors or indirect interactions between dislocations gliding in parallel planes and sessile dislocation loops. We suggest that collinear interaction, already identified as the primary source of strain hardening in FCC metals, is the main dislocation interaction mechanism in olivine deformed at temperatures below 1000 °C.
ISSN: 1478-6443

hal-01174166
https://hal.archives-ouvertes.fr/hal-01174166
DOI : 10.1080/14786435.2014.1000996

Éditeur(s) : HAL CCSD Elsevier
Résumé : International audience
We analyzed twelve fault gouge samples from the Bogd fault in south-western Mongolia to understand the origin and behavior of clay minerals. The investigation relies on x-ray powder diffraction (XRD), scanning electron microscopy (SEM), transmission electron microscopy (TEM) and high energy synchrotron x-ray diffraction methods to investigate microstructure and preferred orientation. Smectite (montmorillonite), illite-smectite mixed layers, illite-mica and kaolinite are the major clay components, in addition to quartz and feldspars, which are present in all samples. The observations suggest that the protoliths and the fault rocks were highly altered by fluids. The fluid-rock interactions allow clay minerals to form, as well as alter feldspars to precipitate kaolinite and montmorillonite. Thus, newly formed clay minerals are heterogeneously distributed in the fault zone. The decrease of montmorillonite component of some of the highly deformed samples also suggests that dehydration processes during deformation were leading to illite precipitation. Based on synchrotron x-ray diffraction data, the degree of preferred orientation of constituent clay minerals is weak, with maxima for (001) ranging from 1.3 to 2.6 multiples of a random distribution (m.r.d). Co-existing quartz and feldspars have random orientation distributions. Microstructure and texture observations of the gouges from the foliated microscopic zone, alternating with micrometric isotropic clay-rich area, also indicate that the Bogd fault experienced brittle and ductile deformation episodes. The clay minerals may contribute to a slip weakening behavior of the fault.
ISSN: 0191-8141

insu-00667194
https://hal-insu.archives-ouvertes.fr/insu-00667194
DOI : 10.1016/j.jsg.2011.10.006

Éditeur(s) : HAL CCSD
Résumé : International audience
In most rock mechanical testing devices, the texture developed during deformation is only observed after the failure, and the detailed mapping of cracks and pore structure evolution, just before or after rupture, remains speculative. Computerized X-ray tomography (CT X-ray) offers a way to observe 'in vivo' some characteristics of a tested sample in the course of its deformation. Therefore we developed an X-ray transparent tri-axial cell that was included in a medical purpose X-ray scanner with a volumetric resolution of the order of 1 mm3. This technique is described and applied to a porous calcarenite, which is regularly scanned for porosity evolution during mechanical testing. The experiment is performed at low confining pressure (2-10 MPa). During testing, an axial load is applied until failure and is then progressively decreased. In these experiments, the peak stress is preceded by phase of diffuse dilatancy. After failure localization, the axial stress is unloaded until the stress reaches isotropy. During this unloading phase, the previous diffuse dilatancy is lost due to an elastic relaxation. Then, the stress is completely relaxed. At this moment the dilatancy increases again and the residual dilatancy is larger than that reached at the peak stress. When observed "post mortem" at microscopic scale, the dilatant structures are comprised of cataclastic deformation bands and cracks in some larger crystals.
International Journal of Rock Mechanics and Mining Science

hal-00767563
https://hal.archives-ouvertes.fr/hal-00767563
DOI : 10.1016/j.ijrmms.2012.07.020

Éditeur(s) : HAL CCSD Czech Phycological Society
Résumé : International audience
As first observed on a group of taxa related to Cocconeis peltoides Hustedt, several other Cocconeis eHRenbeRG (Bacillariophyta) taxa possess a row of minute marginal pores on the external side of their sternum valve (SV) connected to small and simple internal portules, or processes. These processes are often located in continuity with the striae, but can also be slightly shifted or distant from the distal areola of the striae. The row of pores is often, but not always, externally separated from the stria by a crista marginalis. Within Cocconeis, and possibly all raphid diatoms, these processes seem unique and restricted to a few taxa. A list is presented of the taxa pertaining (or close) to Cocconeis that are actually known to share these micro–structures. In addition, the morphological variability of the processes is described in detail and a morphological comparison of the taxa is provided. The characteristic features of taxa showing a row of SV processes permit the tentative definition of several morphological groups. It is difficult to prove the taxonomic affiliation of these taxa and demonstrate or refute that these processes are ancestral characters. Some of these taxa are rare and restricted to low latitudes (e.g., tropical taxa) while others are frequent and ubiquitous. Abbreviations: (LM) light microscope, (SEM) scanning electron microscope, (RV) raphe valve, (SV) sternum valve, (RVVC) raphe valve valvocopula, (SVVC) sternum valve valvocopula
ISSN: 1802-5439

hal-01212883
https://hal-univ-perp.archives-ouvertes.fr/hal-01212883
https://hal-univ-perp.archives-ouvertes.fr/hal-01212883/document
https://hal-univ-perp.archives-ouvertes.fr/hal-01212883/file/fottea_fot-201502-0003.pdf
DOI : 10.5507/fot.2015.015

Éditeur(s) : HAL CCSD Elsevier
Résumé : International audience
The interplay between fault zone cataclasis and cementation is important since both processes can drastically reduce the permeability of faults in porous sandstones. Yet the prediction of fault cementation in high-porosity sandstone reservoirs remains elusive. Nevertheless, this process has rarely been investigated in shallowly buried faults (<2 km; T°<80 °C) where its sealing capacity could be acquired early in the geological history of a reservoir. In this paper, the macro- and microscopic analysis of a fault zone in the porous Cenomanian quartz arenite sands of Provence (France) shows that silica diagenesis occurs in the most intensely-deformed cataclastic parts of the fault zone. This fault zone shows 19–48% of its total thickness occupied by low-porosity quartz-cemented cataclastic shear bands whose porosities range from 0 – ca. 5%. The analysis of the weathering profile around the fault zone reveals the presence of groundwater silcretes in the form of tabular, tightly silicified concretions cross-cut by the fault. Detailed transmitted light, cold-cathodoluminescence and scanning electron microscopy analyses of the silica cements (from the fault and the silcrete) reveal that all the silica cements originate from groundwater diagenetic processes. This study therefore shows that silica cementation can occur specifically in fault zones and as groundwater silcrete in the shallow context of a groundwater system, generated at the vicinity of an erosional unconformity.
ISSN: 0191-8141

hal-01278286
https://hal.archives-ouvertes.fr/hal-01278286
DOI : 10.1016/j.jsg.2015.10.001

Éditeur(s) : HAL CCSD Linnean Society of London
Résumé : International audience
The circumscription of Salicaceae has recently been enlarged to include a majority of the species formerly placed in the polyphyletic tropical family Flacourtiaceae. Several studies have reported a peculiar and infrequently formed multilayered structure of tension wood in four of the tropical genera. Tension wood is a tissue produced by trees to restore their vertical orientation, and most studies have focused on trees developing tension wood by means of cellulose-rich, gelatinous fibres, as is known in Populus L. and Salix L. (Salicaceae s.s.). This study aims to determine if the multilayered structure of tension wood is an anatomical characteristic common in other Salicaceae, and if so, how its distribution correlates to phylogenetic relationships. Therefore, we studied the tension wood of 14 genera of Salicaceae as well as two genera of Achariaceae, one genus of Goupiaceae, and one genus of Lacistemataceae, families closely related to Salicaceae or formerly placed in Flacourtiaceae. Opposite wood and tension wood were compared with light microscopy and 3D Laser Scanning Confocal Microscopy. The results indicate that a multilayered structure of tension wood is common in the family except in Salix, Populus, and one of their closest relatives, Idesia polycarpa Maxim. We suggest that tension wood may be a useful anatomical character in understanding phylogenetic relationships in Salicaceae. Further investigation is still needed on the tension wood of several other putatively close relatives of Salix and Populus, in particular Bennettiodendron Merr., Macrohasseltia L.O.Williams and Itoa Hemsl.
ISSN: 0024-4074

hal-01392845
https://hal.archives-ouvertes.fr/hal-01392845
https://hal.archives-ouvertes.fr/hal-01392845/document
https://hal.archives-ouvertes.fr/hal-01392845/file/GhislainEtAl2016_MultylayerTWSalicaceae_Bot_Jsur the_Lin_Soc_HALssfig.pdf
DOI : 10.1111/boj.12471